MD12G1095100.v1.1

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002064
Physical Location & Seq
Forward (+)
12916524 .. 12919513
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1095100.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGATCAGTTTCACAGACCTGTACCACGTCCTCACTGCCGTTGTGCCGCTGTACGTGGCCATGATCTTAGCCTACGGCTCCGTGAAATGGTGGAAAATCTTCAGCCCCGACCAATGCTCGGGCATCAACAGGTTCGTCGCTCTGTTCGCAGTTCCTCTGCTCTCCTTCCACTTCATCTCCACCAACAACCCCTACGCCATGAACTACAGGTTCATAGCGGCTGACACCCTTCAGAAAATCATCGTCCTGGCGGTCCTGGCTGTTTGGTCGAGAACAAGCGCCAGAGGCTCTCTCGAATGGTCCATCACTCTCTTCTCCCTCTCCACTCTGCCCAACACTCTGGTCATGGGGATACCACTTTTGAAGGGCATGTACGGCGACGCCTCCGGCAGCCTCATGGTCCAAATCGTCGTCCTCCAGTGCATCATTTGGTACACTCTCATGCTCTTCCTTTTCGAGTATAGAGGTGCCCGCCTCCTCATTGGTGAACAGTTCCCGGACACCGCCGGCTCCATCATTTCCTTCACGGTCGATTCCGACATCATATCCTTGGACGGGAAAGAGCCGTTGCAGACAGAGGCCGAGGTAGGAGAAGACGGGAAGCTCCATGTCACTGTCAGAAAATCGACGAGCACTCGCTCCGAAATCCACTCCAGAAGATCCCACGGACCCAACTCCGGCGTGTCCTTAACCCCAAGGCCTTCTAATCTCACCAATGCGGAGATTTATAGCCTTCAGTCTTCGAGAAACCCGACTCCCAGAGGCTCCAGCTTTAACCACACTGATTTTTACTCCATGGTGAACGGGAAAAACATGAGCAATGCCAGTCCGAGACAATCGCACTACGGCAATGTGGGGTCTGATGAGGAGGCCGGAGGAGTTGGGGGGGCCAGAGGACGAGCAAATGGGGGGAATTCGGGGTATCCGGCGCCTCCCAGTGCCGGAATTTTCTCTCCGGTGACCGGACCCTCTGCCGGAGCCAAGAAGAAACCCATTGGGGCTGATGGTGGCAAAGACCTTCACATGTTTGTTTGGAGCTCAAACAATTCGCCTGCGTCGGAAGGAGGCATTCATGTTTTCAGAGGAGGAGAATATGGCAATGAACTTGCTGGGGTTGCTCCCCACTACAAAGATTATGATGAGTTTGGCCGAAATGAGTTCAGCTTTGACAACAGGCCGGCTTCAAACGAGGCAGATCGGGAAGGTCCGGGGCTTTCCAAGTTGGGTTCGAGCTCCAGCACTGAACTCCACCCCAAGACAGCTAATGGTGGTGAATCAAAGACCGCAGCCATGCCGCCTGCTAGCGTTATGACCCGGCTTATTCTAATTATGGTGTGGCGAAAACTGATTAGGAACCCAAACACTTACTCAAGCCTCATTGGCCTCACCTGGTCTTTGGTCTCCTACAAGTGGCACATTGTTATGCCTGCGATTGTAGCCCGCTCGATTGCCATTCTGTCTGATGCAGGTCTTGGAATGGCCATGTTTAGTCTTGGCTTGTTCATGGCTTTACAACCAAGGATGATTGCTTGCGGAAATACAATTGCTTCCTTCGCCATGGCAGTTCGATTCCTTACAGGGCCGGCTGTCATGGCGGCGGCCTCAATTGCCGTTGGACTGCGAGGTGTTCTGTTGCACATTGCCATTGTACAGGCAGCTCTTCCACAAGGGATTGTTCCGTTTGTCTTTTCTAAGGAATACAATGTTCATCCAAACATACTAAGCACCGGGGTTATCTTTGGAATGCTAATCGCTCTTCCGATCACATTGGTTTACTACATCTTGCTAGGGCTTTGA

Protein Analysis

599

Amino Acids

64.36

Weight (kDa)

9.1

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 9 - 593 5.5e-124 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1457
AccB1I GGYRCC 2 cut(s) 467, 928
AccBSI CCGCTC 1 cut(s) 1443
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 3 cut(s) 57, 1147, 1479
AcsI RAATTY 2 cut(s) 913, 945
AcuI CTGAAG 3 cut(s) 85, 215, 719
AcyI GRCGYC 2 cut(s) 381, 929
AfaI GTAC 5 cut(s) 23, 53, 374, 434, 1650
AfiI CCNNNNNNNGG 8 cut(s) 87, 118, 555, 677, 941, 962, 974, 1410
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 2 cut(s) 364, 1185
AjiI CACGTC 1 cut(s) 28
AjnI CCWGG 3 cut(s) 246, 255, 1388
AluBI AGCT 7 cut(s) 604, 771, 1038, 1164, 1233, 1262, 1658
AluI AGCT 7 cut(s) 604, 771, 1038, 1164, 1233, 1262, 1658
Alw21I GWGCWC 3 cut(s) 635, 1040, 1235
Alw26I GTCTC 2 cut(s) 826, 1405
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 118
ApeKI GCWGC 3 cut(s) 390, 1286, 1655
ApoI RAATTY 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 2 cut(s) 281, 931
AspS9I GGNCC 8 cut(s) 253, 300, 400, 668, 888, 965, 1205, 1580
AsuC2I CCSGG 4 cut(s) 497, 1209, 1315, 1729
AsuHPI GGTGA 6 cut(s) 497, 703, 811, 970, 1283, 1378
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 2 cut(s) 59, 1481
BanI GGYRCC 2 cut(s) 467, 928
BanII GRGCYC 2 cut(s) 1040, 1235
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BbsI GAAGAC 2 cut(s) 600, 732
Bbv12I GWGCWC 3 cut(s) 635, 1040, 1235
BbvI GCAGC 3 cut(s) 402, 1298, 1667
BccI CCATC 3 cut(s) 311, 521, 998
BceAI ACGGC 6 cut(s) 23, 91, 391, 550, 862, 1595
BciT130I CCWGG 3 cut(s) 248, 257, 1390
BciVI GTATCC 2 cut(s) 345, 933
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 497, 1209, 1315, 1729
BcoDI GTCTC 2 cut(s) 826, 1405
BfaI CTAG 2 cut(s) 1302, 1787
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 2 cut(s) 282, 932
BfuAI ACCTGC 1 cut(s) 1457
BfuI GTATCC 2 cut(s) 345, 933
BglI GCCNNNNNGGC 1 cut(s) 1380
BisI GCNGC 8 cut(s) 47, 219, 391, 1287, 1295, 1596, 1599, 1656
BlsI GCNGC 8 cut(s) 48, 220, 392, 1288, 1296, 1597, 1600, 1657
Bme1390I CCNGG 7 cut(s) 248, 257, 497, 1209, 1315, 1390, 1729
Bme18I GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
BmeT110I CYCGRG 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 8 cut(s) 253, 300, 400, 668, 888, 965, 1205, 1580
BmrFI CCNGG 7 cut(s) 248, 257, 497, 1209, 1315, 1390, 1729
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 3 cut(s) 132, 432, 1453
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 1 cut(s) 930
BpiI GAAGAC 2 cut(s) 600, 732
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 4 cut(s) 401, 637, 751, 1219
BpuEI CTTGAG 1 cut(s) 1354
BpuMI CCSGG 4 cut(s) 497, 1209, 1315, 1729
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 2 cut(s) 381, 929
BsaI GGTCTC 1 cut(s) 1405
BsaJI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 1208, 1517, 1557, 1728
BsaWI WCCGGW 2 cut(s) 955, 962
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 8 cut(s) 87, 118, 555, 677, 941, 962, 974, 1410
Bse118I RCCGGY 3 cut(s) 506, 1177, 1582
Bse1I ACTGG 3 cut(s) 418, 825, 936
Bse3DI GCAATG 4 cut(s) 826, 856, 1105, 1638
BseBI CCWGG 3 cut(s) 248, 257, 1390
BseDI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 1208, 1517, 1557, 1728
BseGI GGATG 2 cut(s) 1527, 1708
BseLI CCNNNNNNNGG 8 cut(s) 87, 118, 555, 677, 941, 962, 974, 1410
BseMI GCAATG 4 cut(s) 826, 856, 1105, 1638
BseNI ACTGG 3 cut(s) 418, 825, 936
BseRI GAGGAG 5 cut(s) 467, 881, 891, 1098, 1101
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 3 cut(s) 402, 1298, 1667
BseYI CCCAGC 1 cut(s) 1109
Bsh1285I CGRYCG 1 cut(s) 531
BshNI GGYRCC 2 cut(s) 467, 928
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 3 cut(s) 635, 1040, 1235
BsiHKCI CYCGRG 1 cut(s) 118
BslI CCNNNNNNNGG 8 cut(s) 87, 118, 555, 677, 941, 962, 974, 1410
BsmAI GTCTC 2 cut(s) 826, 1405
BsmI GAATGC 2 cut(s) 1068, 1749
Bso31I GGTCTC 1 cut(s) 1405
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 4 cut(s) 472, 635, 1040, 1235
Bsp1407I TGTACA 1 cut(s) 1648
Bsp143I GATC 5 cut(s) 3, 63, 659, 1195, 1761
Bsp19I CCATGG 2 cut(s) 795, 1557
BspMI ACCTGC 1 cut(s) 1457
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 1 cut(s) 654
BspQI GCTCTTC 3 cut(s) 452, 1665, 1761
BspT107I GGYRCC 2 cut(s) 467, 928
BspTNI GGTCTC 1 cut(s) 1405
BsrBI CCGCTC 1 cut(s) 1443
BsrDI GCAATG 4 cut(s) 826, 856, 1105, 1638
BsrFI RCCGGY 3 cut(s) 506, 1177, 1582
BsrGI TGTACA 1 cut(s) 1648
BsrI ACTGG 3 cut(s) 418, 825, 936
BssAI RCCGGY 3 cut(s) 506, 1177, 1582
BssECI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 1208, 1517, 1557, 1728
BssMI GATC 5 cut(s) 3, 63, 659, 1195, 1761
BssNI GRCGYC 2 cut(s) 381, 929
BssT1I CCWWGG 5 cut(s) 549, 695, 795, 1517, 1557
Bst2UI CCWGG 3 cut(s) 248, 257, 1390
Bst4CI ACNGT 3 cut(s) 492, 529, 616
Bst6I CTCTTC 4 cut(s) 317, 452, 1665, 1761
BstACI GRCGYC 2 cut(s) 381, 929
BstAUI TGTACA 1 cut(s) 1648
BstBAI YACGTR 1 cut(s) 55
BstDEI CTNAG 3 cut(s) 67, 1692, 1721
BstDSI CCRYGG 3 cut(s) 664, 795, 1557
BstEII GGTNACC 1 cut(s) 958
BstF5I GGATG 2 cut(s) 1527, 1708
BstH2I RGCGCY 2 cut(s) 282, 932
BstHHI GCGC 2 cut(s) 281, 931
BstKTI GATC 5 cut(s) 6, 66, 662, 1198, 1764
BstMAI GTCTC 2 cut(s) 826, 1405
BstMBI GATC 5 cut(s) 3, 63, 659, 1195, 1761
BstMCI CGRYCG 1 cut(s) 531
BstNI CCWGG 3 cut(s) 248, 257, 1390
BstNSI RCATGY 2 cut(s) 373, 1027
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 7 cut(s) 246, 255, 495, 1207, 1313, 1388, 1727
BstSFI CTRYAG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 3 cut(s) 402, 1298, 1667
BstV2I GAAGAC 2 cut(s) 600, 732
BstX2I RGATCY 1 cut(s) 659
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 659
BsuI GTATCC 2 cut(s) 345, 933
BtgI CCRYGG 3 cut(s) 664, 795, 1557
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 2 cut(s) 1527, 1708
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 6 cut(s) 33, 425, 612, 780, 943, 1239
BveI ACCTGC 1 cut(s) 1457
CfoI GCGC 2 cut(s) 281, 931
Cfr10I RCCGGY 3 cut(s) 506, 1177, 1582
Cfr13I GGNCC 8 cut(s) 253, 300, 400, 668, 888, 965, 1205, 1580
CseI GACGC 2 cut(s) 389, 1044
CsiI ACCWGGT 1 cut(s) 1388
Csp6I GTAC 5 cut(s) 22, 52, 373, 433, 1649
CviQI GTAC 5 cut(s) 22, 52, 373, 433, 1649
DdeI CTNAG 3 cut(s) 67, 1692, 1721
DinI GGCGCC 1 cut(s) 930
DpnI GATC 5 cut(s) 5, 65, 661, 1197, 1763
DpnII GATC 5 cut(s) 3, 63, 659, 1195, 1761
EaeI YGGCCR 3 cut(s) 57, 1147, 1479
Eam1104I CTCTTC 4 cut(s) 317, 452, 1665, 1761
EarI CTCTTC 4 cut(s) 317, 452, 1665, 1761
Ecl136II GAGCTC 2 cut(s) 1038, 1233
Eco130I CCWWGG 5 cut(s) 549, 695, 795, 1517, 1557
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 2 cut(s) 1040, 1235
Eco31I GGTCTC 1 cut(s) 1405
Eco47I GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
Eco53kI GAGCTC 2 cut(s) 1038, 1233
Eco57I CTGAAG 3 cut(s) 85, 215, 719
Eco88I CYCGRG 1 cut(s) 118
Eco91I GGTNACC 1 cut(s) 958
EcoICRI GAGCTC 2 cut(s) 1038, 1233
EcoO65I GGTNACC 1 cut(s) 958
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 3 cut(s) 246, 255, 1388
EcoT14I CCWWGG 5 cut(s) 549, 695, 795, 1517, 1557
EcoT38I GRGCYC 2 cut(s) 1040, 1235
EgeI GGCGCC 1 cut(s) 930
EheI GGCGCC 1 cut(s) 930
ErhI CCWWGG 5 cut(s) 549, 695, 795, 1517, 1557
FauI CCCGC 2 cut(s) 479, 1448
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 8 cut(s) 47, 219, 391, 1287, 1295, 1596, 1599, 1656
FokI GGATG 2 cut(s) 1534, 1695
FriOI GRGCYC 2 cut(s) 1040, 1235
Fsp4HI GCNGC 8 cut(s) 47, 219, 391, 1287, 1295, 1596, 1599, 1656
FspBI CTAG 2 cut(s) 1302, 1787
GlaI GCGC 2 cut(s) 280, 930
GluI GCNGC 8 cut(s) 47, 219, 391, 1287, 1295, 1596, 1599, 1656
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 4 cut(s) 401, 637, 751, 1219
HaeII RGCGCY 2 cut(s) 282, 932
HgaI GACGC 2 cut(s) 389, 1044
HhaI GCGC 2 cut(s) 281, 931
Hin1I GRCGYC 2 cut(s) 381, 929
Hin6I GCGC 2 cut(s) 279, 929
HinP1I GCGC 2 cut(s) 279, 929
HinfI GANTC 4 cut(s) 533, 754, 1274, 1569
HphI GGTGA 6 cut(s) 497, 703, 811, 970, 1283, 1378
Hpy166II GTNNAC 4 cut(s) 435, 488, 802, 1774
Hpy188III TCNNGA 5 cut(s) 270, 293, 654, 744, 1199
Hpy8I GTNNAC 4 cut(s) 435, 488, 802, 1774
Hpy99I CGWCG 5 cut(s) 140, 383, 413, 631, 1060
HpyCH4III ACNGT 3 cut(s) 492, 529, 616
HpyCH4IV ACGT 2 cut(s) 27, 54
HpyCH4V TGCA 4 cut(s) 423, 571, 1466, 1636
HpyF3I CTNAG 3 cut(s) 67, 1692, 1721
HpySE526I ACGT 2 cut(s) 27, 54
Hsp92I GRCGYC 2 cut(s) 381, 929
HspAI GCGC 2 cut(s) 279, 929
KasI GGCGCC 1 cut(s) 928
KroI GCCGGC 3 cut(s) 506, 1177, 1582
KroNI GCCGGC 3 cut(s) 508, 1179, 1584
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 63, 659, 1195, 1761
LguI GCTCTTC 3 cut(s) 452, 1665, 1761
LmnI GCTCC 9 cut(s) 83, 515, 609, 644, 770, 977, 1035, 1123, 1238
Lsp1109I GCAGC 3 cut(s) 402, 1298, 1667
LweI GCATC 3 cut(s) 132, 432, 1453
MabI ACCWGGT 1 cut(s) 1388
MaeI CTAG 2 cut(s) 1302, 1787
MaeII ACGT 2 cut(s) 27, 54
MaeIII GTNAC 2 cut(s) 610, 958
MalI GATC 5 cut(s) 5, 65, 661, 1197, 1763
MbiI CCGCTC 1 cut(s) 1443
MboI GATC 5 cut(s) 3, 63, 659, 1195, 1761
MboII GAAGA 9 cut(s) 91, 304, 439, 605, 669, 732, 997, 1652, 1748
MfeI CAATTG 2 cut(s) 1542, 1605
MflI RGATCY 1 cut(s) 659
MhlI GDGCHC 4 cut(s) 472, 635, 1040, 1235
MlsI TGGCCA 2 cut(s) 59, 1481
MluCI AATT 6 cut(s) 913, 945, 1045, 1326, 1542, 1605
MluNI TGGCCA 2 cut(s) 59, 1481
Mly113I GGCGCC 1 cut(s) 929
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 3 cut(s) 561, 1038, 1594
Mox20I TGGCCA 2 cut(s) 59, 1481
MroNI GCCGGC 3 cut(s) 506, 1177, 1582
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 2 cut(s) 59, 1481
MseI TTAA 2 cut(s) 689, 774
MslI CAYNNNNRTG 2 cut(s) 440, 1421
Msp20I TGGCCA 2 cut(s) 59, 1481
MspA1I CMGCKG 1 cut(s) 49
MspR9I CCNGG 7 cut(s) 248, 257, 497, 1209, 1315, 1390, 1729
MunI CAATTG 2 cut(s) 1542, 1605
Mva1269I GAATGC 2 cut(s) 1068, 1749
MvaI CCWGG 3 cut(s) 248, 257, 1390
NaeI GCCGGC 3 cut(s) 508, 1179, 1584
NarI GGCGCC 1 cut(s) 929
NciI CCSGG 4 cut(s) 497, 1209, 1315, 1729
NcoI CCATGG 2 cut(s) 795, 1557
NdeII GATC 5 cut(s) 3, 63, 659, 1195, 1761
NgoMIV GCCGGC 3 cut(s) 506, 1177, 1582
NheI GCTAGC 1 cut(s) 1301
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 2 cut(s) 610, 958
NspI RCATGY 2 cut(s) 373, 1027
PceI AGGCCT 1 cut(s) 700
PciI ACATGT 1 cut(s) 1023
PciSI GCTCTTC 3 cut(s) 452, 1665, 1761
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PctI GAATGC 2 cut(s) 1068, 1749
PdiI GCCGGC 3 cut(s) 508, 1179, 1584
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 533, 1274, 1569
PfoI TCCNGGA 1 cut(s) 495
PkrI GCNGC 8 cut(s) 48, 220, 392, 1288, 1296, 1597, 1600, 1657
PleI GAGTC 1 cut(s) 748
PluTI GGCGCC 1 cut(s) 932
PpsI GAGTC 1 cut(s) 748
Ppu21I YACGTR 1 cut(s) 55
PscI ACATGT 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 1040, 1235
Psp6I CCWGG 3 cut(s) 246, 255, 1388
PspEI GGTNACC 1 cut(s) 958
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 3 cut(s) 246, 255, 1388
PspPI GGNCC 8 cut(s) 253, 300, 400, 668, 888, 965, 1205, 1580
PsuI RGATCY 1 cut(s) 659
RsaI GTAC 5 cut(s) 23, 53, 374, 434, 1650
RsaNI GTAC 5 cut(s) 22, 52, 373, 433, 1649
RseI CAYNNNNRTG 2 cut(s) 440, 1421
SacI GAGCTC 2 cut(s) 1040, 1235
SapI GCTCTTC 3 cut(s) 452, 1665, 1761
SaqAI TTAA 2 cut(s) 689, 774
SatI GCNGC 8 cut(s) 47, 219, 391, 1287, 1295, 1596, 1599, 1656
Sau3AI GATC 5 cut(s) 3, 63, 659, 1195, 1761
Sau96I GGNCC 8 cut(s) 253, 300, 400, 668, 888, 965, 1205, 1580
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 7 cut(s) 248, 257, 497, 1209, 1315, 1390, 1729
SduI GDGCHC 4 cut(s) 472, 635, 1040, 1235
SexAI ACCWGGT 1 cut(s) 1388
SfaNI GCATC 3 cut(s) 132, 432, 1453
SfcI CTRYAG 1 cut(s) 205
SfoI GGCGCC 1 cut(s) 930
SinI GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
SmiMI CAYNNNNRTG 2 cut(s) 440, 1421
SmlI CTYRAG 1 cut(s) 1369
SmoI CTYRAG 1 cut(s) 1369
Sse9I AATT 6 cut(s) 913, 945, 1045, 1326, 1542, 1605
SseBI AGGCCT 1 cut(s) 700
SspDI GGCGCC 1 cut(s) 928
SspMI CTAG 2 cut(s) 1302, 1787
SstI GAGCTC 2 cut(s) 1040, 1235
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 7 cut(s) 246, 255, 495, 1207, 1313, 1388, 1727
StyI CCWWGG 5 cut(s) 549, 695, 795, 1517, 1557
TaaI ACNGT 3 cut(s) 492, 529, 616
TaiI ACGT 2 cut(s) 30, 57
TaqI TCGA 9 cut(s) 269, 294, 456, 531, 626, 743, 1229, 1445, 1567
TasI AATT 6 cut(s) 913, 945, 1045, 1326, 1542, 1605
TatI WGTACW 1 cut(s) 1648
TauI GCSGC 5 cut(s) 49, 221, 1297, 1598, 1601
TfiI GAWTC 3 cut(s) 533, 1274, 1569
Tru1I TTAA 2 cut(s) 689, 774
Tru9I TTAA 2 cut(s) 689, 774
TscAI CASTG 6 cut(s) 40, 425, 619, 787, 943, 1246
TseFI GTSAC 2 cut(s) 610, 958
TseI GCWGC 3 cut(s) 390, 1286, 1655
Tsp45I GTSAC 2 cut(s) 610, 958
TspDTI ATGAA 7 cut(s) 163, 202, 215, 1061, 1116, 1492, 1697
TspGWI ACGGA 3 cut(s) 70, 681, 1668
TspRI CASTG 6 cut(s) 40, 425, 619, 787, 943, 1246
VpaK11BI GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
XapI RAATTY 2 cut(s) 913, 945
XceI RCATGY 2 cut(s) 373, 1027
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 1302, 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.