Rh7CG016000

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002064
Physical Location & Seq
Reverse (-)
1188593 .. 1190875
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG016000.1

Sequence Viewer

Length: 711 bp
ATGGCCGCCGGCCGGAACTCTAATTTCGGGTCCTCCGACGTTTTCGGAGGGCCGTTGACGGCGTCCAGAGGCCCGACGCCGCGGCCGTCGAATTATGAGGACGACGGGGGCGCCGCCGTGGGTGCTGCTGCAGCTGGCAGCAACAAGCCGAGGTATCATTACCAAGGAGGAGGGAACAATACAACAGCGCATTACCCGGCCCCGAATCCGGGAATGTTCTCGCCTACCACGGCGGCCTCTAAGAACGTTGTCGCTCCCAAGAGGCCTAATGGAAATGGGCCTAATGGGCCGGCGGCTCAGGACGGCGGTAGGGATCTTCATATGTGGCATCTAGAAATGCCAGCAATCATAGCAAAGTCGATAGCCATTCTATCAGACGCAGGGCTTGGGATGGCCATGTTCAGTCTTGGTCTGTTTATGGCTTTACAACCACGGATCATAGCATGTGGAAATTCCATTGCAGCTTTTACTATGGCTGTGAGATTCCTTACAGGTCCAGCTGTCATGGCAGCTGCTTCCATTGCTGTTGGCCTTAGGGGCACTCTCTTACATGTTGCCATTGTCCAGGCAGCTCTACCCCAAGGAATTGTCCCCTTTGTCTTTGCCAAGGAATACAACGTACACCCTGATATTCTCAGCACAGGAGTTATATTCGGCATGTTGATCGCGTTGCCCATTACGCTTGTCTACTACATAATGCTGGGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009505 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009965 GO:0009966 GO:0009987 GO:0010016 GO:0010033 GO:0010051 GO:0010154 GO:0010229 GO:0010252 GO:0010315 GO:0010329 GO:0010338 GO:0010358 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016021 GO:0016323 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0023052 GO:0030054 GO:0030312 GO:0031224 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045177 GO:0045178 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048532 GO:0048583 GO:0048598 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048830 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055044 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0080161 GO:0090567 GO:0090698 GO:0098590 GO:0098796 GO:0098797 GO:0099402 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

236

Amino Acids

24.27

Weight (kDa)

9.37

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 109 - 231 3.1e-40 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 687
AccII CGCG 2 cut(s) 82, 668
AciI CCGC 7 cut(s) 6, 80, 82, 114, 233, 293, 306
AclI AACGTT 1 cut(s) 246
AclWI GGATC 2 cut(s) 321, 443
AcoI YGGCCR 4 cut(s) 3, 10, 83, 393
AcsI RAATTY 1 cut(s) 451
AcyI GRCGYC 3 cut(s) 62, 77, 111
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 3 cut(s) 12, 208, 209
AflIII ACRYGT 1 cut(s) 550
AjnI CCWGG 1 cut(s) 564
AluBI AGCT 5 cut(s) 134, 464, 500, 512, 572
AluI AGCT 5 cut(s) 134, 464, 500, 512, 572
AlwI GGATC 2 cut(s) 321, 443
ApeKI GCWGC 8 cut(s) 125, 128, 131, 138, 461, 509, 512, 569
ApoI RAATTY 1 cut(s) 451
AspLEI GCGC 2 cut(s) 113, 190
AspS9I GGNCC 7 cut(s) 30, 50, 71, 199, 278, 287, 494
AsuC2I CCSGG 2 cut(s) 197, 210
AvaII GGWCC 2 cut(s) 30, 494
AxyI CCTNAGG 1 cut(s) 533
BaeGI GKGCMC 1 cut(s) 542
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 110
BbvI GCAGC 8 cut(s) 112, 115, 143, 150, 473, 499, 521, 581
BccI CCATC 1 cut(s) 385
BceAI ACGGC 6 cut(s) 37, 70, 75, 101, 246, 319
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BciT130I CCWGG 1 cut(s) 566
BcnI CCSGG 2 cut(s) 197, 210
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 114
BglI GCCNNNNNGGC 2 cut(s) 286, 537
Bme1390I CCNGG 3 cut(s) 197, 210, 566
Bme18I GGWCC 2 cut(s) 30, 494
BmgT120I GGNCC 7 cut(s) 30, 50, 71, 199, 278, 287, 494
BmiI GGNNCC 3 cut(s) 31, 112, 201
BmrFI CCNGG 3 cut(s) 197, 210, 566
BmsI GCATC 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 197, 210
BsaHI GRCGYC 3 cut(s) 62, 77, 111
BsaJI CCNNGG 8 cut(s) 80, 117, 149, 163, 228, 431, 580, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 208, 209
Bse118I RCCGGY 2 cut(s) 8, 289
Bse21I CCTNAGG 1 cut(s) 533
Bse3DI GCAATG 2 cut(s) 456, 519
BseBI CCWGG 1 cut(s) 566
BseDI CCNNGG 8 cut(s) 80, 117, 149, 163, 228, 431, 580, 606
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 12, 208, 209
BseMI GCAATG 2 cut(s) 456, 519
BseMII CTCAG 2 cut(s) 311, 649
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 542
BseX3I CGGCCG 2 cut(s) 10, 83
BseXI GCAGC 8 cut(s) 112, 115, 143, 150, 473, 499, 521, 581
BseYI CCCAGC 1 cut(s) 700
Bsh1236I CGCG 2 cut(s) 82, 668
Bsh1285I CGRYCG 2 cut(s) 13, 86
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 2 cut(s) 13, 86
BsiSI CCGG 5 cut(s) 9, 13, 197, 209, 290
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 3 cut(s) 12, 208, 209
BsmFI GGGAC 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 542
Bsp143I GATC 3 cut(s) 313, 435, 663
BspACI CCGC 7 cut(s) 6, 80, 82, 114, 233, 293, 306
BspCNI CTCAG 2 cut(s) 310, 648
BspFNI CGCG 2 cut(s) 82, 668
BspLI GGNNCC 3 cut(s) 31, 112, 201
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 2 cut(s) 321, 443
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 456, 519
BsrFI RCCGGY 2 cut(s) 8, 289
BssAI RCCGGY 2 cut(s) 8, 289
BssECI CCNNGG 8 cut(s) 80, 117, 149, 163, 228, 431, 580, 606
BssMI GATC 3 cut(s) 313, 435, 663
BssNI GRCGYC 3 cut(s) 62, 77, 111
BssT1I CCWWGG 3 cut(s) 163, 580, 606
Bst2UI CCWGG 1 cut(s) 566
BstACI GRCGYC 3 cut(s) 62, 77, 111
BstC8I GCNNGC 4 cut(s) 10, 136, 291, 342
BstDEI CTNAG 4 cut(s) 240, 297, 533, 635
BstDSI CCRYGG 4 cut(s) 80, 117, 228, 431
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 2 cut(s) 82, 668
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 113, 190
BstKTI GATC 3 cut(s) 316, 438, 666
BstMBI GATC 3 cut(s) 313, 435, 663
BstMCI CGRYCG 2 cut(s) 13, 86
BstMWI GCNNNNNNNGC 8 cut(s) 122, 131, 286, 350, 506, 521, 537, 679
BstNI CCWGG 1 cut(s) 566
BstNSI RCATGY 3 cut(s) 447, 554, 661
BstSCI CCNGG 3 cut(s) 195, 208, 564
BstSFI CTRYAG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 542
BstUI CGCG 2 cut(s) 82, 668
BstV1I GCAGC 8 cut(s) 112, 115, 143, 150, 473, 499, 521, 581
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZI CGGCCG 2 cut(s) 10, 83
Bsu36I CCTNAGG 1 cut(s) 533
BtgI CCRYGG 4 cut(s) 80, 117, 228, 431
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 4 cut(s) 10, 136, 291, 342
CfoI GCGC 2 cut(s) 113, 190
Cfr10I RCCGGY 2 cut(s) 8, 289
Cfr13I GGNCC 7 cut(s) 30, 50, 71, 199, 278, 287, 494
Cfr42I CCGCGG 1 cut(s) 83
CseI GACGC 3 cut(s) 51, 85, 386
Csp6I GTAC 1 cut(s) 620
CviAII CATG 5 cut(s) 397, 444, 505, 551, 658
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 4 cut(s) 240, 297, 533, 635
DinI GGCGCC 1 cut(s) 112
DpnI GATC 3 cut(s) 315, 437, 665
DpnII GATC 3 cut(s) 313, 435, 663
EaeI YGGCCR 4 cut(s) 3, 10, 83, 393
EagI CGGCCG 2 cut(s) 10, 83
EclXI CGGCCG 2 cut(s) 10, 83
Eco130I CCWWGG 3 cut(s) 163, 580, 606
Eco147I AGGCCT 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 30, 494
Eco52I CGGCCG 2 cut(s) 10, 83
Eco81I CCTNAGG 1 cut(s) 533
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 3 cut(s) 163, 580, 606
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 163, 580, 606
FaeI CATG 5 cut(s) 400, 447, 508, 554, 661
FaqI GGGAC 1 cut(s) 575
FatI CATG 5 cut(s) 396, 443, 504, 550, 657
FauNDI CATATG 1 cut(s) 321
FblI GTMKAC 1 cut(s) 687
FokI GGATG 1 cut(s) 403
FspBI CTAG 1 cut(s) 332
GlaI GCGC 2 cut(s) 112, 189
GsaI CCCAGC 1 cut(s) 704
HaeII RGCGCY 1 cut(s) 114
HapII CCGG 5 cut(s) 9, 13, 197, 209, 290
HgaI GACGC 3 cut(s) 51, 85, 386
HhaI GCGC 2 cut(s) 113, 190
Hin1I GRCGYC 3 cut(s) 62, 77, 111
Hin1II CATG 5 cut(s) 400, 447, 508, 554, 661
Hin6I GCGC 2 cut(s) 111, 188
HinP1I GCGC 2 cut(s) 111, 188
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 2 cut(s) 205, 483
HpaII CCGG 5 cut(s) 9, 13, 197, 209, 290
Hpy166II GTNNAC 3 cut(s) 57, 622, 688
Hpy188I TCNGA 3 cut(s) 37, 47, 376
Hpy188III TCNNGA 3 cut(s) 66, 299, 332
Hpy8I GTNNAC 3 cut(s) 57, 622, 688
Hpy99I CGWCG 4 cut(s) 41, 79, 91, 107
HpyCH4IV ACGT 3 cut(s) 39, 246, 618
HpyCH4V TGCA 2 cut(s) 131, 461
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 131, 286, 350, 506, 521, 537, 679
HpyF3I CTNAG 4 cut(s) 240, 297, 533, 635
HpySE526I ACGT 3 cut(s) 39, 246, 618
Hsp92I GRCGYC 3 cut(s) 62, 77, 111
Hsp92II CATG 5 cut(s) 400, 447, 508, 554, 661
HspAI GCGC 2 cut(s) 111, 188
KasI GGCGCC 1 cut(s) 110
KroI GCCGGC 2 cut(s) 8, 289
KroNI GCCGGC 2 cut(s) 10, 291
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 3 cut(s) 313, 435, 663
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 8 cut(s) 112, 115, 143, 150, 473, 499, 521, 581
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 332
MaeII ACGT 3 cut(s) 39, 246, 618
MalI GATC 3 cut(s) 315, 437, 665
MboI GATC 3 cut(s) 313, 435, 663
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 542
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 4 cut(s) 22, 91, 451, 585
MluNI TGGCCA 1 cut(s) 395
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 9 cut(s) 41, 43, 62, 91, 144, 161, 164, 247, 255
Mox20I TGGCCA 1 cut(s) 395
MroNI GCCGGC 2 cut(s) 8, 289
MscI TGGCCA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 4 cut(s) 82, 134, 500, 512
MspI CCGG 5 cut(s) 9, 13, 197, 209, 290
MspR9I CCNGG 3 cut(s) 197, 210, 566
MvaI CCWGG 1 cut(s) 566
MvnI CGCG 2 cut(s) 82, 668
MwoI GCNNNNNNNGC 8 cut(s) 122, 131, 286, 350, 506, 521, 537, 679
NaeI GCCGGC 2 cut(s) 10, 291
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 2 cut(s) 197, 210
NdeI CATATG 1 cut(s) 321
NdeII GATC 3 cut(s) 313, 435, 663
NgoMIV GCCGGC 2 cut(s) 8, 289
NlaIII CATG 5 cut(s) 400, 447, 508, 554, 661
NlaIV GGNNCC 3 cut(s) 31, 112, 201
NmeAIII GCCGAG 1 cut(s) 174
NspI RCATGY 3 cut(s) 447, 554, 661
PceI AGGCCT 1 cut(s) 265
PciI ACATGT 1 cut(s) 550
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 83
PdiI GCCGGC 2 cut(s) 10, 291
PfeI GAWTC 2 cut(s) 205, 483
PflFI GACNNNGTC 1 cut(s) 61
PfoI TCCNGGA 1 cut(s) 208
PluTI GGCGCC 1 cut(s) 114
PpuMI RGGWCCY 1 cut(s) 30
PscI ACATGT 1 cut(s) 550
Psp1406I AACGTT 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 3 cut(s) 31, 112, 201
PspPI GGNCC 7 cut(s) 30, 50, 71, 199, 278, 287, 494
PspPPI RGGWCCY 1 cut(s) 30
PstI CTGCAG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 61
PvuII CAGCTG 3 cut(s) 134, 500, 512
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SacII CCGCGG 1 cut(s) 83
Sau3AI GATC 3 cut(s) 313, 435, 663
Sau96I GGNCC 7 cut(s) 30, 50, 71, 199, 278, 287, 494
ScrFI CCNGG 3 cut(s) 197, 210, 566
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 129
SfiI GGCCNNNNNGGCC 1 cut(s) 286
SfoI GGCGCC 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 2 cut(s) 30, 494
Sse9I AATT 4 cut(s) 22, 91, 451, 585
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 7 cut(s) 6, 80, 82, 114, 233, 293, 306
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 332
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 3 cut(s) 195, 208, 564
StyI CCWWGG 3 cut(s) 163, 580, 606
TaiI ACGT 3 cut(s) 42, 249, 621
TaqI TCGA 2 cut(s) 89, 359
TasI AATT 4 cut(s) 22, 91, 451, 585
TauI GCSGC 6 cut(s) 8, 82, 85, 116, 236, 296
TfiI GAWTC 2 cut(s) 205, 483
TseI GCWGC 8 cut(s) 125, 128, 131, 138, 461, 509, 512, 569
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 30, 494
XapI RAATTY 1 cut(s) 451
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 3 cut(s) 447, 554, 661
XmiI GTMKAC 1 cut(s) 687
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.