Rh2DG342200

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002064
Physical Location & Seq
Reverse (-)
44565659 .. 44568847
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG342200.1

Sequence Viewer

Length: 1470 bp
ATGATCAGCTTGGGAGACCTGTACCACGTTCTGACAGCTGTTGTGCCACTCTATGTGGCCATGATTTTGGCCTACGGGTCAGTGAAGTGGTGGAAGATCTTCAGCCCCGATCAATGTTCTGGCATCAACCGCTTTGTGGCCCTGTTCGCCGTTCCTCTGCTCTCCTTCCACTTCATCTCCACCAACAATCCATACGCCATGAACTACAGGTTCATAGCCGCAGACACTCTTCAAAAAGTCATAGTCTTGGCGGTCCTAGCCATATGGTCAAGAACCAGCTCAAGAGGCTCTCTGGAGTGGTCCATCACTCTCTTTTCCCTTTCTACTCTTCCCAACACTCTGGTGATGGGCATACCCTTGCTAAAGGGCATGTATGGAGACTCCTCAGGGACCTTAATGGTCCAAATAGTTGTCCTCCAGTGCATTGTTTGGTATACTCTGATGCTCTTCTTGTTCGAGTACAGAGGAGCTAGGCTCTTGATTGGTGAGCAGTTCCCGGACACTGCTGCCTCCATCGTTTCCTTTCGCGTTGATTCCGATATCGTTTCTTTAGATGGGAAAGAGCCATTACAGACTGAAGCCCAGCTCGGAGAAGATGGGAAACTCCATGTCACTGTTAGAAAATCCACCAGCTCTCGCTCTGAAATCTTCTCCAAACCATCCCTTGGACCCAACACTGGTGTTTCCTGTACCCCAAGGCTTTCCAACTTGACTAATGCTGAAATCTACAGCCTTCAGTCTTCTAGAAACCCAACTCCCAGGGGTTCCAATTTTAGTCACACTGATTTCTATGCAATGGTGAATGGGAAGAACATGAGTAATGTGAGTCCAAGGCTGTCGAATTACGGCTATGATGAAGAGGCCACCGGTGTCGGCATGAGACCACCAAGTTCCATGCAGGGGTATCCTACGCCTCCCAGCACCGGAATATTCTCTCCGGTGGCTGGACCCAATGCAACTGCCAAGATAAAGCCCAATGGGACTGATGCCACTGGTAAGGATCTTCACATGTTTGTTTGGAGCTCGAGTGCTTCTCCAGTCTCAGAAGGAGGCATTCATGTTTTCAGAGGTGGAGTTGCTCACCAGAAAGATCACGATGAGTTTGGGCGAGATGAGTTCAGCTTCGATAACAGACCAGTTTCTAATGGGATTGATCGAGAGGGTCCGGTGCTTTCCAAGTTGGGTTCCAGCTCCTCCACCACTGAACTCCACCCAAAGACTTGTCATGGTGAATCAAAGGCCACAGCAATGCCACCAGCTAGCGTTATGACCCGTCTCATTCTCATTATGGTCTGGAGGAAACTCATTAGAAACCCAAACACGTACTCCAGCCTTATTGGCCTTACCTGGTCTTTAGTCTCATATAAGTGGAACATTGTTATGCCTGCAATCATAGCCCGTTCAATAGCCATTCTTTCTGATGCCGGTCTTGGAATGGCCATGTTTAGCCTTGGTATGTTAGGATTGTAG

Protein Analysis

489

Amino Acids

53.29

Weight (kDa)

8.87

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 9 - 486 7.6e-90 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 665
AccI GTMKAC 1 cut(s) 434
AccII CGCG 1 cut(s) 528
AciI CCGC 3 cut(s) 130, 219, 251
AclWI GGATC 1 cut(s) 1008
AcoI YGGCCR 2 cut(s) 57, 1437
AcuI CTGAAG 3 cut(s) 85, 597, 719
AfaI GTAC 4 cut(s) 23, 461, 691, 1325
AfiI CCNNNNNNNGG 6 cut(s) 136, 665, 677, 900, 923, 944
AflIII ACRYGT 2 cut(s) 1008, 1320
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 2 cut(s) 233, 1404
AjnI CCWGG 2 cut(s) 758, 1346
AleI CACNNNNGTG 1 cut(s) 341
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 6 cut(s) 9, 372, 874, 1045, 1280, 1363
AlwI GGATC 1 cut(s) 1008
Ama87I CYCGRG 1 cut(s) 1024
AoxI GGCC 7 cut(s) 57, 69, 138, 861, 1239, 1339, 1437
ApeKI GCWGC 1 cut(s) 506
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 8 cut(s) 139, 253, 300, 390, 400, 668, 947, 1163
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 5 cut(s) 355, 497, 811, 1073, 1241
AsuNHI GCTAGC 1 cut(s) 1259
AvaI CYCGRG 1 cut(s) 1024
AvaII GGWCC 7 cut(s) 253, 300, 390, 400, 668, 947, 1163
AxyI CCTNAGG 1 cut(s) 385
BalI TGGCCA 2 cut(s) 59, 1439
BanII GRGCYC 1 cut(s) 1025
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 1 cut(s) 493
BccI CCATC 6 cut(s) 311, 340, 521, 548, 590, 667
BceAI ACGGC 2 cut(s) 134, 862
BciT130I CCWGG 2 cut(s) 760, 1348
BciVI GTATCC 1 cut(s) 915
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 6 cut(s) 9, 372, 874, 1045, 1280, 1363
BfaI CTAG 4 cut(s) 257, 471, 744, 1260
BfmI CTRYAG 2 cut(s) 205, 727
BfuI GTATCC 1 cut(s) 915
BglI GCCNNNNNGGC 1 cut(s) 1338
BglII AGATCT 1 cut(s) 96
BisI GCNGC 2 cut(s) 219, 507
BlsI GCNGC 2 cut(s) 220, 508
Bme1390I CCNGG 3 cut(s) 497, 760, 1348
Bme18I GGWCC 7 cut(s) 253, 300, 390, 400, 668, 947, 1163
BmeT110I CYCGRG 1 cut(s) 1024
BmgT120I GGNCC 8 cut(s) 139, 253, 300, 390, 400, 668, 947, 1163
BmiI GGNNCC 6 cut(s) 391, 670, 766, 949, 1164, 1186
BmrFI CCNGG 3 cut(s) 497, 760, 1348
BmsI GCATC 4 cut(s) 132, 432, 976, 1411
BmtI GCTAGC 1 cut(s) 1263
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 4 cut(s) 459, 491, 1018, 1050
BpmI CTGGAG 5 cut(s) 314, 401, 1020, 1312, 1315
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 1323
BsaI GGTCTC 2 cut(s) 9, 874
BsaJI CCNNGG 6 cut(s) 664, 695, 758, 759, 830, 1450
BsaWI WCCGGW 4 cut(s) 866, 923, 937, 1165
BsaXI ACNNNNNCTCC 6 cut(s) 161, 191, 1012, 1042, 1310, 1340
Bsc4I CCNNNNNNNGG 6 cut(s) 136, 665, 677, 900, 923, 944
Bse118I RCCGGY 2 cut(s) 866, 1424
Bse1I ACTGG 5 cut(s) 418, 682, 997, 1037, 1136
Bse21I CCTNAGG 1 cut(s) 385
Bse3DI GCAATG 2 cut(s) 801, 1254
BseBI CCWGG 2 cut(s) 760, 1348
BseDI CCNNGG 6 cut(s) 664, 695, 758, 759, 830, 1450
BseGI GGATG 1 cut(s) 659
BseLI CCNNNNNNNGG 6 cut(s) 136, 665, 677, 900, 923, 944
BseMI GCAATG 2 cut(s) 801, 1254
BseMII CTCAG 2 cut(s) 399, 1056
BseNI ACTGG 5 cut(s) 418, 682, 997, 1037, 1136
BseRI GAGGAG 3 cut(s) 373, 480, 1183
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 2 cut(s) 582, 917
Bsh1236I CGCG 1 cut(s) 528
BshFI GGCC 7 cut(s) 59, 71, 140, 863, 1241, 1341, 1439
BshTI ACCGGT 1 cut(s) 866
BsiHKAI GWGCWC 1 cut(s) 1025
BsiHKCI CYCGRG 1 cut(s) 1024
BsiSI CCGG 6 cut(s) 497, 867, 924, 938, 1166, 1425
BslFI GGGAC 2 cut(s) 403, 994
BslI CCNNNNNNNGG 6 cut(s) 136, 665, 677, 900, 923, 944
BsmAI GTCTC 6 cut(s) 9, 372, 874, 1045, 1280, 1363
BsmBI CGTCTC 1 cut(s) 1280
BsmFI GGGAC 2 cut(s) 403, 994
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 7 cut(s) 59, 71, 140, 863, 1241, 1341, 1439
Bso31I GGTCTC 2 cut(s) 9, 874
BsoBI CYCGRG 1 cut(s) 1024
Bsp1286I GDGCHC 1 cut(s) 1025
Bsp143I GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
BspACI CCGC 3 cut(s) 130, 219, 251
BspANI GGCC 7 cut(s) 59, 71, 140, 863, 1241, 1341, 1439
BspCNI CTCAG 2 cut(s) 398, 1055
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 6 cut(s) 391, 670, 766, 949, 1164, 1186
BspOI GCTAGC 1 cut(s) 1263
BspPI GGATC 1 cut(s) 1008
BspQI GCTCTTC 1 cut(s) 452
BspTNI GGTCTC 2 cut(s) 9, 874
BsrDI GCAATG 2 cut(s) 801, 1254
BsrFI RCCGGY 2 cut(s) 866, 1424
BsrI ACTGG 5 cut(s) 418, 682, 997, 1037, 1136
BssAI RCCGGY 2 cut(s) 866, 1424
BssECI CCNNGG 6 cut(s) 664, 695, 758, 759, 830, 1450
BssMI GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
BssNAI GTATAC 1 cut(s) 435
BssT1I CCWWGG 4 cut(s) 664, 695, 830, 1450
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 760, 1348
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 4 cut(s) 234, 333, 452, 852
BstBAI YACGTR 1 cut(s) 1323
BstC8I GCNNGC 2 cut(s) 1261, 1386
BstDEI CTNAG 2 cut(s) 385, 1042
BstF5I GGATG 1 cut(s) 659
BstFNI CGCG 1 cut(s) 528
BstKTI GATC 6 cut(s) 6, 99, 112, 1003, 1093, 1156
BstMAI GTCTC 6 cut(s) 9, 372, 874, 1045, 1280, 1363
BstMBI GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
BstMWI GCNNNNNNNGC 6 cut(s) 129, 146, 257, 285, 1338, 1394
BstNI CCWGG 2 cut(s) 760, 1348
BstNSI RCATGY 2 cut(s) 373, 1012
BstSCI CCNGG 3 cut(s) 495, 758, 1346
BstSFI CTRYAG 2 cut(s) 205, 727
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 2 cut(s) 96, 1000
BstXI CCANNNNNNTGG 2 cut(s) 67, 340
BstYI RGATCY 2 cut(s) 96, 1000
BstZ17I GTATAC 1 cut(s) 435
Bsu36I CCTNAGG 1 cut(s) 385
BsuI GTATCC 1 cut(s) 915
BsuRI GGCC 7 cut(s) 59, 71, 140, 863, 1241, 1341, 1439
BtsCI GGATG 1 cut(s) 659
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 8 cut(s) 87, 425, 501, 612, 675, 780, 990, 1200
Cac8I GCNNGC 2 cut(s) 1261, 1386
Cfr10I RCCGGY 2 cut(s) 866, 1424
Cfr13I GGNCC 8 cut(s) 139, 253, 300, 390, 400, 668, 947, 1163
CsiI ACCWGGT 1 cut(s) 1346
Csp6I GTAC 4 cut(s) 22, 460, 690, 1324
CspAI ACCGGT 1 cut(s) 866
CviQI GTAC 4 cut(s) 22, 460, 690, 1324
DdeI CTNAG 2 cut(s) 385, 1042
DpnI GATC 6 cut(s) 5, 98, 111, 1002, 1092, 1155
DpnII GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
EaeI YGGCCR 2 cut(s) 57, 1437
Eam1104I CTCTTC 4 cut(s) 234, 333, 452, 852
EarI CTCTTC 4 cut(s) 234, 333, 452, 852
Ecl136II GAGCTC 1 cut(s) 1023
Eco130I CCWWGG 4 cut(s) 664, 695, 830, 1450
Eco24I GRGCYC 1 cut(s) 1025
Eco31I GGTCTC 2 cut(s) 9, 874
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 7 cut(s) 253, 300, 390, 400, 668, 947, 1163
Eco53kI GAGCTC 1 cut(s) 1023
Eco57I CTGAAG 3 cut(s) 85, 597, 719
Eco81I CCTNAGG 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 1024
EcoICRI GAGCTC 1 cut(s) 1023
EcoO109I RGGNCCY 1 cut(s) 390
EcoRII CCWGG 2 cut(s) 758, 1346
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 4 cut(s) 664, 695, 830, 1450
EcoT38I GRGCYC 1 cut(s) 1025
ErhI CCWWGG 4 cut(s) 664, 695, 830, 1450
Esp3I CGTCTC 1 cut(s) 1280
FaqI GGGAC 2 cut(s) 403, 994
FauNDI CATATG 1 cut(s) 263
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 2 cut(s) 219, 507
FokI GGATG 1 cut(s) 646
FriOI GRGCYC 1 cut(s) 1025
Fsp4HI GCNGC 2 cut(s) 219, 507
FspBI CTAG 4 cut(s) 257, 471, 744, 1260
GluI GCNGC 2 cut(s) 219, 507
GsaI CCCAGC 2 cut(s) 586, 921
GsuI CTGGAG 5 cut(s) 314, 401, 1020, 1312, 1315
HaeIII GGCC 7 cut(s) 59, 71, 140, 863, 1241, 1341, 1439
HapII CCGG 6 cut(s) 497, 867, 924, 938, 1166, 1425
HinfI GANTC 4 cut(s) 380, 533, 826, 1232
HpaII CCGG 6 cut(s) 497, 867, 924, 938, 1166, 1425
HphI GGTGA 5 cut(s) 355, 497, 811, 1073, 1241
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 8 cut(s) 33, 441, 538, 590, 643, 1045, 1067, 1420
Hpy188III TCNNGA 8 cut(s) 270, 282, 293, 478, 744, 1094, 1157, 1294
Hpy8I GTNNAC 1 cut(s) 435
HpyAV CCTTC 3 cut(s) 175, 743, 1040
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 2 cut(s) 27, 1322
HpyCH4V TGCA 5 cut(s) 423, 794, 898, 956, 1388
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 146, 257, 285, 1338, 1394
HpyF3I CTNAG 2 cut(s) 385, 1042
HpySE526I ACGT 2 cut(s) 27, 1322
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
LguI GCTCTTC 1 cut(s) 452
LmnI GCTCC 3 cut(s) 467, 1020, 1196
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 4 cut(s) 132, 432, 976, 1411
MabI ACCWGGT 1 cut(s) 1346
MaeI CTAG 4 cut(s) 257, 471, 744, 1260
MaeII ACGT 2 cut(s) 27, 1322
MaeIII GTNAC 2 cut(s) 610, 776
MalI GATC 6 cut(s) 5, 98, 111, 1002, 1092, 1155
MboI GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
MflI RGATCY 2 cut(s) 96, 1000
MhlI GDGCHC 1 cut(s) 1025
MlsI TGGCCA 2 cut(s) 59, 1439
MluCI AATT 2 cut(s) 769, 841
MluNI TGGCCA 2 cut(s) 59, 1439
MlyI GAGTC 2 cut(s) 374, 835
MmeI TCCRAC 1 cut(s) 729
Mox20I TGGCCA 2 cut(s) 59, 1439
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 2 cut(s) 59, 1439
MseI TTAA 1 cut(s) 395
MslI CAYNNNNRTG 4 cut(s) 341, 1247, 1366, 1379
Msp20I TGGCCA 2 cut(s) 59, 1439
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 6 cut(s) 497, 867, 924, 938, 1166, 1425
MspR9I CCNGG 3 cut(s) 497, 760, 1348
Mva1269I GAATGC 1 cut(s) 1053
MvaI CCWGG 2 cut(s) 760, 1348
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 6 cut(s) 129, 146, 257, 285, 1338, 1394
NciI CCSGG 1 cut(s) 497
NdeI CATATG 1 cut(s) 263
NdeII GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
NheI GCTAGC 1 cut(s) 1259
NlaIV GGNNCC 6 cut(s) 391, 670, 766, 949, 1164, 1186
NmuCI GTSAC 2 cut(s) 610, 776
NspI RCATGY 2 cut(s) 373, 1012
OliI CACNNNNGTG 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 1024
PasI CCCWGGG 1 cut(s) 759
PciI ACATGT 1 cut(s) 1008
PciSI GCTCTTC 1 cut(s) 452
PctI GAATGC 1 cut(s) 1053
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 533, 1232
PflMI CCANNNNNTGG 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 495
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 2 cut(s) 220, 508
PleI GAGTC 2 cut(s) 374, 834
PpsI GAGTC 2 cut(s) 374, 834
Ppu21I YACGTR 1 cut(s) 1323
PpuMI RGGWCCY 1 cut(s) 390
PscI ACATGT 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 758, 1346
PspFI CCCAGC 2 cut(s) 582, 917
PspGI CCWGG 2 cut(s) 758, 1346
PspN4I GGNNCC 6 cut(s) 391, 670, 766, 949, 1164, 1186
PspPI GGNCC 8 cut(s) 139, 253, 300, 390, 400, 668, 947, 1163
PspPPI RGGWCCY 1 cut(s) 390
PspXI VCTCGAGB 1 cut(s) 1024
PsuI RGATCY 2 cut(s) 96, 1000
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 4 cut(s) 23, 461, 691, 1325
RsaNI GTAC 4 cut(s) 22, 460, 690, 1324
RseI CAYNNNNRTG 4 cut(s) 341, 1247, 1366, 1379
SacI GAGCTC 1 cut(s) 1025
SapI GCTCTTC 1 cut(s) 452
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 2 cut(s) 219, 507
Sau3AI GATC 6 cut(s) 3, 96, 109, 1000, 1090, 1153
Sau96I GGNCC 8 cut(s) 139, 253, 300, 390, 400, 668, 947, 1163
SchI GAGTC 2 cut(s) 374, 835
ScrFI CCNGG 3 cut(s) 497, 760, 1348
SduI GDGCHC 1 cut(s) 1025
SexAI ACCWGGT 1 cut(s) 1346
SfaNI GCATC 4 cut(s) 132, 432, 976, 1411
SfcI CTRYAG 2 cut(s) 205, 727
Sfr274I CTCGAG 1 cut(s) 1024
SgrAI CRCCGGYG 1 cut(s) 866
SinI GGWCC 7 cut(s) 253, 300, 390, 400, 668, 947, 1163
SlaI CTCGAG 1 cut(s) 1024
SmiMI CAYNNNNRTG 4 cut(s) 341, 1247, 1366, 1379
SmlI CTYRAG 2 cut(s) 280, 1024
SmoI CTYRAG 2 cut(s) 280, 1024
Sse9I AATT 2 cut(s) 769, 841
SsiI CCGC 3 cut(s) 130, 219, 251
SspI AATATT 1 cut(s) 930
SspMI CTAG 4 cut(s) 257, 471, 744, 1260
SstI GAGCTC 1 cut(s) 1025
StyD4I CCNGG 3 cut(s) 495, 758, 1346
StyI CCWWGG 4 cut(s) 664, 695, 830, 1450
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 2 cut(s) 30, 1325
TaqI TCGA 5 cut(s) 456, 839, 1025, 1125, 1156
TasI AATT 2 cut(s) 769, 841
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 533, 1232
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 8 cut(s) 87, 425, 508, 619, 682, 787, 997, 1207
TseFI GTSAC 2 cut(s) 610, 776
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 2 cut(s) 610, 776
TspDTI ATGAA 5 cut(s) 163, 202, 215, 870, 1046
TspRI CASTG 8 cut(s) 87, 425, 508, 619, 682, 787, 997, 1207
Van91I CCANNNNNTGG 1 cut(s) 665
VpaK11BI GGWCC 7 cut(s) 253, 300, 390, 400, 668, 947, 1163
XbaI TCTAGA 1 cut(s) 743
XceI RCATGY 2 cut(s) 373, 1012
XhoI CTCGAG 1 cut(s) 1024
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 4 cut(s) 257, 471, 744, 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.