Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002064

Physical Location & Seq

Genomic Coordinates Forward (+)

12984459 .. 12988104

Gene Structure 3646 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1090900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGATCAGTTTCACAGACCTGTACCACGTCCTCACCGCCGTTGTGCCGCTGTACGTGGCCATGATCTTAGCCTACGGCTCCGTGAAATGGTGGAAGATCTTCAGCCCCGACCAATGCTCCGGCATCAACCGCTTCGTCGCTCTGTTTGCAGTTCCTCTGCTCTCCTTCCACTTCATCTCCACCAACAACCCCTATGCCATGAACTACAGGTTCATAGCGGCTGACACGCTTCAGAAAGTCATCGTCCTGTTGGTCCTAGCCGTGTGGTCGAGAACCAGCGCCAGAGGCTCTCTCGAGTGGTCCATCACTCTTTTCTCCCTCTCCACTCTGCCCAACACTCTGGTCATGGGGATACCCCTTCTGAAGGGCATGTACGGTGACGCCTCCGGCAGCCTCATGGTCCAAATCGTCGTCCTCCAGTGTATCATTTGGTACACTCTTATGCTGTTCCTCTTCGAGTACAGGGGCGCTCGCCTCCTCATTGGTGAACAGTTCCCGGACACCGCCGGCTCCATCATTTCCTTCAGGGTCGATTCCGACATCATTTCCTTGGACGGAAAAGAGCCGTTGCAGACAGAGGCCGAGGTCGGAGAAGACGGGAAGCTCCATGTCACTGTCAGAAAATCGACGAGCTCTCGCTCCGAAATATACTCCAGAAGATCCCACGGACCCAACTCCGGCGTGTCCTTAACCCCAAGGCCTTCTAATCTCACCAATGCTGAGATTTACAGCCTTCAGTCTTCGCGAAACCCGACTCCCAGAGGCTCCAGCTTTAACCACACTGATTTCTACTCCATGGTGAACGGGAAAAACATGAGCAATGCGAGTCCGAGGCAGTCGAATTACGGGAATGTGGGGTTTGATGAGGAGGCTAGAGGAGTTGGGGGGCCCAGAGGAGCAGCAAATGTGGGGAGTTTGGGGTATCCGGCGCCTCCCAGTGCCGGGATATTCTCTCCGGTGACCGGACCCGCCGCTGGGGCAAAGAAGCAACCCAATGGCACCGATGGTGGCAAAGACCTACACATGTTTGTTTGGAGCTCAAGCGCTTCGCCTGTGTCGGAAGGAGGCATTCATGTTTTCAGAGGAGAATATGGCAATGAACTTGCTGGGGTTGCTGCTCTCCACAAAGGTTTTTATTATGATGAGTTTGGCCGAGATGAGTTCAGCTTCGACAACAGGCCGGCTTCAAACGGGGCTGATCGGGAAGGTCCGGTGCTTTCCAAGTTGGGTTCGAGCTCCACCTCTGAACTCCACCCCAAGACAGCTAATGGTGGTGAATCAAAGACCACAGCCATGCCGCCTGCTAGCGTTATGACCCGACTCATTCTTATTATGGTTTGGCGAAAACTGATTAGGAACCCAAACACTTACTCCAGCCTCATTGGCCTCACCTGGTCTCTGGTCTCCTACAAGTGGCACATTGTTATGCCTGCAATTGTAGCCCGTTCGATAGCCATTCTGTCTGATGCAGGTCTTGGAATGGCCATGTTTAGTCTTGGCTTGTTCATGGCTTTACAACCACGGATGATTGCTTGCGGAAATACAGTTGCTTCCTTTGCTATGGCAGTTCGATTCCTCACTGGGCCGGCTGTCATGGCAGCGGCCTCAATTGCTGTTGGACTGCGAGGTGTTTTGTTGCACATTGCCATTGTACAGGCAGCTCTTCCACAAGGGATTGTTCCGTTTGTCTTTGCCAAGGAATACAATGTTCATCCTGACATACTAAGCACCGGGGTTATCTTCGGAATGCTAATCGCTCTTCCGATCACATTGGTTTACTACATCTTGCTAGGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006810 GO:0008150 GO:0009914 GO:0009926 GO:0009966 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0022857 GO:0023051 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0071944 GO:0080161

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

600

Amino Acids

64.64

Weight (kDa)

9.1

Isoelectric Point (pI)

35.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	9 - 594	3.6e-125	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002064)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73590
fragaria_vesca	FvH4_5g17310 FvH4_5g17310 FvH4_5g17310 FvH4_5g17310 FvH4_6g25530
malus_domestica	MD06G1226800.v1.1 MD12G1095100.v1.1 MD14G1090900.v1.1 MD14G1236300.v1.1
prunus_persica	Prupe.5G233100_v2.0.a1 Prupe.5G233100_v2.0.a1 Prupe.7G031400_v2.0.a1
pyrus_communis	pycom06g20310 pycom12g08100 pycom14g08280 pycom14g19730
rosa_chinensis	RchiOBHm_Chr2g0125421 RchiOBHm_Chr7g0178461
rosa_laevigata	RLG00000005446 RLG00000018862
rosa_multiflora	Rmu_co8305407.1_g000001 Rmu_co8511139.1_g000001 Rmu_sc0010235.1_g000007
rosa_roxburghii	Rroxscaffold_2G00118040 Rroxscaffold_3G00274900
rosa_rugosa	Rorug02G0257200 Rorug02G0257300 Rorug06G0417200 Rorug06G0417200
rosa_samantha	Rh2AG315900 Rh2BG323700 Rh2CG305900 Rh2DG342200 Rh3AG201700 Rh5AG183300 Rh5DG535000 Rh7AG015800 Rh7BG015200 Rh7CG016000 Rh7DG015600 Rh7DG015700
rosa_wichuraiana	Rw2G025490 Rw7G001240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1460
AccB1I	GGYRCC	2 cut(s)	928, 998
AccII	CGCG	1 cut(s)	745
AclWI	GGATC	1 cut(s)	654
AcoI	YGGCCR	3 cut(s)	57, 1150, 1482
AcuI	CTGAAG	5 cut(s)	85, 215, 383, 508, 719
AcyI	GRCGYC	2 cut(s)	381, 929
AfaI	GTAC	6 cut(s)	23, 53, 374, 434, 461, 1653
AfeI	AGCGCT	1 cut(s)	1045
AfiI	CCNNNNNNNGG	9 cut(s)	87, 364, 677, 941, 942, 962, 974, 975, 1413
AflIII	ACRYGT	1 cut(s)	1023
AgsI	TTSAA	1 cut(s)	1188
AjiI	CACGTC	1 cut(s)	28
AjnI	CCWGG	1 cut(s)	1391
AluBI	AGCT	8 cut(s)	604, 633, 771, 1038, 1167, 1236, 1265, 1661
AluI	AGCT	8 cut(s)	604, 633, 771, 1038, 1167, 1236, 1265, 1661
Alw21I	GWGCWC	3 cut(s)	635, 1040, 1238
Alw26I	GTCTC	2 cut(s)	1401, 1408
AlwI	GGATC	1 cut(s)	654
Ama87I	CYCGRG	1 cut(s)	293
Aor51HI	AGCGCT	1 cut(s)	1045
ApaI	GGGCCC	1 cut(s)	891
ApeKI	GCWGC	5 cut(s)	390, 899, 1115, 1598, 1658
Asp700I	GAANNNNTTC	1 cut(s)	98
AspLEI	GCGC	4 cut(s)	281, 470, 931, 1046
AspS9I	GGNCC	9 cut(s)	253, 300, 400, 668, 887, 888, 965, 1208, 1583
AsuC2I	CCSGG	3 cut(s)	497, 943, 1732
AsuHPI	GGTGA	8 cut(s)	25, 389, 497, 703, 811, 970, 1286, 1381
AsuNHI	GCTAGC	1 cut(s)	1304
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	6 cut(s)	253, 300, 400, 668, 965, 1208
BaeGI	GKGCMC	1 cut(s)	891
BalI	TGGCCA	2 cut(s)	59, 1484
BanI	GGYRCC	2 cut(s)	928, 998
BanII	GRGCYC	4 cut(s)	635, 891, 1040, 1238
BarI	GAAGNNNNNNTAC	2 cut(s)	356, 388
BbsI	GAAGAC	2 cut(s)	600, 732
Bbv12I	GWGCWC	3 cut(s)	635, 1040, 1238
BbvI	GCAGC	5 cut(s)	402, 911, 1102, 1610, 1670
BccI	CCATC	3 cut(s)	311, 521, 998
BceAI	ACGGC	4 cut(s)	23, 91, 245, 550
BciT130I	CCWGG	1 cut(s)	1393
BciVI	GTATCC	2 cut(s)	345, 933
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	3 cut(s)	497, 943, 1732
BcoDI	GTCTC	2 cut(s)	1401, 1408
BfaI	CTAG	4 cut(s)	257, 873, 1305, 1790
BfmI	CTRYAG	1 cut(s)	205
BfoI	RGCGCY	4 cut(s)	282, 471, 932, 1047
BfuAI	ACCTGC	1 cut(s)	1460
BfuI	GTATCC	2 cut(s)	345, 933
BglI	GCCNNNNNGGC	2 cut(s)	977, 1383
BglII	AGATCT	1 cut(s)	96
Bme1390I	CCNGG	4 cut(s)	497, 943, 1393, 1732
Bme18I	GGWCC	6 cut(s)	253, 300, 400, 668, 965, 1208
BmeT110I	CYCGRG	1 cut(s)	293
BmgBI	CACGTC	1 cut(s)	28
BmgT120I	GGNCC	9 cut(s)	253, 300, 400, 668, 887, 888, 965, 1208, 1583
BmrFI	CCNGG	4 cut(s)	497, 943, 1393, 1732
BmrI	ACTGGG	2 cut(s)	930, 1590
BmsI	GCATC	2 cut(s)	132, 1456
BmtI	GCTAGC	1 cut(s)	1308
BmuI	ACTGGG	2 cut(s)	930, 1590
BpiI	GAAGAC	2 cut(s)	600, 732
BplI	GAGNNNNNCTC	2 cut(s)	276, 308
BpmI	CTGGAG	4 cut(s)	401, 637, 751, 1357
BpuEI	CTTGAG	1 cut(s)	1024
BpuMI	CCSGG	3 cut(s)	497, 943, 1732
BsaAI	YACGTR	1 cut(s)	55
BsaHI	GRCGYC	2 cut(s)	381, 929
BsaI	GGTCTC	2 cut(s)	1401, 1408
BsaJI	CCNNGG	9 cut(s)	549, 582, 664, 695, 795, 830, 1520, 1695, 1731
BsaWI	WCCGGW	3 cut(s)	955, 962, 1210
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 161, 191, 1355, 1385
Bsc4I	CCNNNNNNNGG	9 cut(s)	87, 364, 677, 941, 942, 962, 974, 975, 1413
Bse118I	RCCGGY	3 cut(s)	506, 1180, 1585
Bse1I	ACTGG	3 cut(s)	418, 936, 1585
Bse3DI	GCAATG	3 cut(s)	826, 1102, 1641
BseBI	CCWGG	1 cut(s)	1393
BseDI	CCNNGG	9 cut(s)	549, 582, 664, 695, 795, 830, 1520, 1695, 1731
BseGI	GGATG	2 cut(s)	1530, 1711
BseLI	CCNNNNNNNGG	9 cut(s)	87, 364, 677, 941, 942, 962, 974, 975, 1413
BseMI	GCAATG	3 cut(s)	826, 1102, 1641
BseMII	CTCAG	1 cut(s)	711
BseNI	ACTGG	3 cut(s)	418, 936, 1585
BseRI	GAGGAG	5 cut(s)	467, 881, 891, 909, 1098
BseSI	GKGCMC	1 cut(s)	891
BseXI	GCAGC	5 cut(s)	402, 911, 1102, 1610, 1670
BseYI	CCCAGC	2 cut(s)	974, 1106
Bsh1236I	CGCG	1 cut(s)	745
BshNI	GGYRCC	2 cut(s)	928, 998
BsiHKAI	GWGCWC	3 cut(s)	635, 1040, 1238
BsiHKCI	CYCGRG	1 cut(s)	293
BslI	CCNNNNNNNGG	9 cut(s)	87, 364, 677, 941, 942, 962, 974, 975, 1413
BsmAI	GTCTC	2 cut(s)	1401, 1408
BsmI	GAATGC	2 cut(s)	1068, 1752
Bso31I	GGTCTC	2 cut(s)	1401, 1408
BsoBI	CYCGRG	1 cut(s)	293
Bsp120I	GGGCCC	1 cut(s)	887
Bsp1286I	GDGCHC	4 cut(s)	635, 891, 1040, 1238
Bsp1407I	TGTACA	1 cut(s)	1651
Bsp143I	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
Bsp19I	CCATGG	1 cut(s)	795
Bsp68I	TCGCGA	1 cut(s)	745
BspCNI	CTCAG	1 cut(s)	712
BspFNI	CGCG	1 cut(s)	745
BspMI	ACCTGC	1 cut(s)	1460
BspOI	GCTAGC	1 cut(s)	1308
BspPI	GGATC	1 cut(s)	654
BspQI	GCTCTTC	2 cut(s)	1668, 1764
BspT107I	GGYRCC	2 cut(s)	928, 998
BspTNI	GGTCTC	2 cut(s)	1401, 1408
BsrDI	GCAATG	3 cut(s)	826, 1102, 1641
BsrFI	RCCGGY	3 cut(s)	506, 1180, 1585
BsrGI	TGTACA	1 cut(s)	1651
BsrI	ACTGG	3 cut(s)	418, 936, 1585
BssAI	RCCGGY	3 cut(s)	506, 1180, 1585
BssECI	CCNNGG	9 cut(s)	549, 582, 664, 695, 795, 830, 1520, 1695, 1731
BssMI	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
BssNI	GRCGYC	2 cut(s)	381, 929
BssT1I	CCWWGG	4 cut(s)	549, 695, 795, 1695
Bst2UI	CCWGG	1 cut(s)	1393
Bst4CI	ACNGT	4 cut(s)	377, 492, 616, 1546
Bst6I	CTCTTC	3 cut(s)	458, 1668, 1764
BstACI	GRCGYC	2 cut(s)	381, 929
BstAUI	TGTACA	1 cut(s)	1651
BstBAI	YACGTR	1 cut(s)	55
BstC8I	GCNNGC	8 cut(s)	472, 508, 1182, 1302, 1306, 1431, 1534, 1587
BstDEI	CTNAG	3 cut(s)	67, 720, 1724
BstDSI	CCRYGG	3 cut(s)	664, 795, 1520
BstEII	GGTNACC	1 cut(s)	958
BstENI	CCTNNNNNAGG	1 cut(s)	362
BstF5I	GGATG	2 cut(s)	1530, 1711
BstFNI	CGCG	1 cut(s)	745
BstH2I	RGCGCY	4 cut(s)	282, 471, 932, 1047
BstHHI	GCGC	4 cut(s)	281, 470, 931, 1046
BstKTI	GATC	6 cut(s)	6, 66, 99, 662, 1201, 1767
BstMAI	GTCTC	2 cut(s)	1401, 1408
BstMBI	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
BstNI	CCWGG	1 cut(s)	1393
BstNSI	RCATGY	2 cut(s)	373, 1027
BstPI	GGTNACC	1 cut(s)	958
BstSCI	CCNGG	4 cut(s)	495, 941, 1391, 1730
BstSFI	CTRYAG	1 cut(s)	205
BstSLI	GKGCMC	1 cut(s)	891
BstUI	CGCG	1 cut(s)	745
BstV1I	GCAGC	5 cut(s)	402, 911, 1102, 1610, 1670
BstV2I	GAAGAC	2 cut(s)	600, 732
BstX2I	RGATCY	2 cut(s)	96, 659
BstXI	CCANNNNNNTGG	1 cut(s)	340
BstYI	RGATCY	2 cut(s)	96, 659
BsuI	GTATCC	2 cut(s)	345, 933
BtgI	CCRYGG	3 cut(s)	664, 795, 1520
BtrI	CACGTC	1 cut(s)	28
BtsCI	GGATG	2 cut(s)	1530, 1711
BtsIMutI	CAGTG	5 cut(s)	425, 612, 780, 943, 1578
BtuMI	TCGCGA	1 cut(s)	745
BveI	ACCTGC	1 cut(s)	1460
Cac8I	GCNNGC	8 cut(s)	472, 508, 1182, 1302, 1306, 1431, 1534, 1587
CfoI	GCGC	4 cut(s)	281, 470, 931, 1046
Cfr10I	RCCGGY	3 cut(s)	506, 1180, 1585
Cfr13I	GGNCC	9 cut(s)	253, 300, 400, 668, 887, 888, 965, 1208, 1583
CseI	GACGC	1 cut(s)	389
CsiI	ACCWGGT	1 cut(s)	1391
Csp6I	GTAC	6 cut(s)	22, 52, 373, 433, 460, 1652
CviQI	GTAC	6 cut(s)	22, 52, 373, 433, 460, 1652
DdeI	CTNAG	3 cut(s)	67, 720, 1724
DinI	GGCGCC	1 cut(s)	930
DpnI	GATC	6 cut(s)	5, 65, 98, 661, 1200, 1766
DpnII	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
EaeI	YGGCCR	3 cut(s)	57, 1150, 1482
Eam1104I	CTCTTC	3 cut(s)	458, 1668, 1764
EarI	CTCTTC	3 cut(s)	458, 1668, 1764
Ecl136II	GAGCTC	3 cut(s)	633, 1038, 1236
Eco130I	CCWWGG	4 cut(s)	549, 695, 795, 1695
Eco147I	AGGCCT	1 cut(s)	700
Eco24I	GRGCYC	4 cut(s)	635, 891, 1040, 1238
Eco31I	GGTCTC	2 cut(s)	1401, 1408
Eco47I	GGWCC	6 cut(s)	253, 300, 400, 668, 965, 1208
Eco47III	AGCGCT	1 cut(s)	1045
Eco53kI	GAGCTC	3 cut(s)	633, 1038, 1236
Eco57I	CTGAAG	5 cut(s)	85, 215, 383, 508, 719
Eco88I	CYCGRG	1 cut(s)	293
Eco91I	GGTNACC	1 cut(s)	958
EcoICRI	GAGCTC	3 cut(s)	633, 1038, 1236
EcoNI	CCTNNNNNAGG	1 cut(s)	362
EcoO109I	RGGNCCY	1 cut(s)	887
EcoO65I	GGTNACC	1 cut(s)	958
EcoRII	CCWGG	1 cut(s)	1391
EcoT14I	CCWWGG	4 cut(s)	549, 695, 795, 1695
EcoT38I	GRGCYC	4 cut(s)	635, 891, 1040, 1238
EgeI	GGCGCC	1 cut(s)	930
EheI	GGCGCC	1 cut(s)	930
ErhI	CCWWGG	4 cut(s)	549, 695, 795, 1695
FauI	CCCGC	1 cut(s)	976
FbaI	TGATCA	1 cut(s)	3
FokI	GGATG	2 cut(s)	1537, 1698
FriOI	GRGCYC	4 cut(s)	635, 891, 1040, 1238
FspBI	CTAG	4 cut(s)	257, 873, 1305, 1790
GlaI	GCGC	4 cut(s)	280, 469, 930, 1045
GsaI	CCCAGC	2 cut(s)	978, 1110
GsuI	CTGGAG	4 cut(s)	401, 637, 751, 1357
HaeII	RGCGCY	4 cut(s)	282, 471, 932, 1047
HgaI	GACGC	1 cut(s)	389
HhaI	GCGC	4 cut(s)	281, 470, 931, 1046
Hin1I	GRCGYC	2 cut(s)	381, 929
Hin6I	GCGC	4 cut(s)	279, 468, 929, 1044
HinP1I	GCGC	4 cut(s)	279, 468, 929, 1044
HinfI	GANTC	6 cut(s)	533, 754, 826, 1277, 1320, 1572
HphI	GGTGA	8 cut(s)	25, 389, 497, 703, 811, 970, 1286, 1381
Hpy166II	GTNNAC	4 cut(s)	435, 488, 802, 1777
Hpy188III	TCNNGA	6 cut(s)	270, 293, 654, 744, 1202, 1715
Hpy8I	GTNNAC	4 cut(s)	435, 488, 802, 1777
Hpy99I	CGWCG	3 cut(s)	140, 413, 631
HpyAV	CCTTC	8 cut(s)	175, 358, 368, 532, 711, 743, 1055, 1199
HpyCH4III	ACNGT	4 cut(s)	377, 492, 616, 1546
HpyCH4IV	ACGT	2 cut(s)	27, 54
HpyCH4V	TGCA	5 cut(s)	149, 571, 1433, 1469, 1639
HpyF3I	CTNAG	3 cut(s)	67, 720, 1724
HpySE526I	ACGT	2 cut(s)	27, 54
Hsp92I	GRCGYC	2 cut(s)	381, 929
HspAI	GCGC	4 cut(s)	279, 468, 929, 1044
KasI	GGCGCC	1 cut(s)	928
KroI	GCCGGC	3 cut(s)	506, 1180, 1585
KroNI	GCCGGC	3 cut(s)	508, 1182, 1587
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
LguI	GCTCTTC	2 cut(s)	1668, 1764
LmnI	GCTCC	9 cut(s)	83, 122, 515, 609, 644, 770, 896, 1035, 1241
Lsp1109I	GCAGC	5 cut(s)	402, 911, 1102, 1610, 1670
LweI	GCATC	2 cut(s)	132, 1456
MabI	ACCWGGT	1 cut(s)	1391
MaeI	CTAG	4 cut(s)	257, 873, 1305, 1790
MaeII	ACGT	2 cut(s)	27, 54
MaeIII	GTNAC	3 cut(s)	377, 610, 958
MalI	GATC	6 cut(s)	5, 65, 98, 661, 1200, 1766
MboI	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
MboII	GAAGA	9 cut(s)	91, 106, 445, 605, 669, 732, 1655, 1732, 1751
MfeI	CAATTG	2 cut(s)	1434, 1608
MflI	RGATCY	2 cut(s)	96, 659
MhlI	GDGCHC	4 cut(s)	635, 891, 1040, 1238
MlsI	TGGCCA	2 cut(s)	59, 1484
MluCI	AATT	3 cut(s)	841, 1434, 1608
MluNI	TGGCCA	2 cut(s)	59, 1484
Mly113I	GGCGCC	1 cut(s)	929
MlyI	GAGTC	3 cut(s)	748, 835, 1314
MmeI	TCCRAC	4 cut(s)	561, 568, 1038, 1597
Mox20I	TGGCCA	2 cut(s)	59, 1484
MroNI	GCCGGC	3 cut(s)	506, 1180, 1585
MroXI	GAANNNNTTC	1 cut(s)	98
MscI	TGGCCA	2 cut(s)	59, 1484
MseI	TTAA	2 cut(s)	689, 774
MslI	CAYNNNNRTG	3 cut(s)	440, 1292, 1424
Msp20I	TGGCCA	2 cut(s)	59, 1484
MspA1I	CMGCKG	3 cut(s)	49, 974, 1601
MspR9I	CCNGG	4 cut(s)	497, 943, 1393, 1732
MunI	CAATTG	2 cut(s)	1434, 1608
Mva1269I	GAATGC	2 cut(s)	1068, 1752
MvaI	CCWGG	1 cut(s)	1393
MvnI	CGCG	1 cut(s)	745
NaeI	GCCGGC	3 cut(s)	508, 1182, 1587
NarI	GGCGCC	1 cut(s)	929
NciI	CCSGG	3 cut(s)	497, 943, 1732
NcoI	CCATGG	1 cut(s)	795
NdeII	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
NgoMIV	GCCGGC	3 cut(s)	506, 1180, 1585
NheI	GCTAGC	1 cut(s)	1304
NmeAIII	GCCGAG	2 cut(s)	607, 1178
NmuCI	GTSAC	3 cut(s)	377, 610, 958
NruI	TCGCGA	1 cut(s)	745
NspI	RCATGY	2 cut(s)	373, 1027
PaeR7I	CTCGAG	1 cut(s)	293
PceI	AGGCCT	1 cut(s)	700
PciI	ACATGT	1 cut(s)	1023
PciSI	GCTCTTC	2 cut(s)	1668, 1764
PcsI	WCGNNNNNNNCGW	1 cut(s)	749
PctI	GAATGC	2 cut(s)	1068, 1752
PdiI	GCCGGC	3 cut(s)	508, 1182, 1587
PdmI	GAANNNNTTC	1 cut(s)	98
PfeI	GAWTC	3 cut(s)	533, 1277, 1572
PfoI	TCCNGGA	1 cut(s)	495
PleI	GAGTC	3 cut(s)	748, 834, 1314
PluTI	GGCGCC	1 cut(s)	932
PpsI	GAGTC	3 cut(s)	748, 834, 1314
Ppu21I	YACGTR	1 cut(s)	55
PscI	ACATGT	1 cut(s)	1023
Psp124BI	GAGCTC	3 cut(s)	635, 1040, 1238
Psp6I	CCWGG	1 cut(s)	1391
PspEI	GGTNACC	1 cut(s)	958
PspFI	CCCAGC	2 cut(s)	974, 1106
PspGI	CCWGG	1 cut(s)	1391
PspOMI	GGGCCC	1 cut(s)	887
PspPI	GGNCC	9 cut(s)	253, 300, 400, 668, 887, 888, 965, 1208, 1583
PsuI	RGATCY	2 cut(s)	96, 659
RruI	TCGCGA	1 cut(s)	745
RsaI	GTAC	6 cut(s)	23, 53, 374, 434, 461, 1653
RsaNI	GTAC	6 cut(s)	22, 52, 373, 433, 460, 1652
RseI	CAYNNNNRTG	3 cut(s)	440, 1292, 1424
SacI	GAGCTC	3 cut(s)	635, 1040, 1238
SapI	GCTCTTC	2 cut(s)	1668, 1764
SaqAI	TTAA	2 cut(s)	689, 774
Sau3AI	GATC	6 cut(s)	3, 63, 96, 659, 1198, 1764
Sau96I	GGNCC	9 cut(s)	253, 300, 400, 668, 887, 888, 965, 1208, 1583
SchI	GAGTC	3 cut(s)	748, 835, 1314
ScrFI	CCNGG	4 cut(s)	497, 943, 1393, 1732
SduI	GDGCHC	4 cut(s)	635, 891, 1040, 1238
SexAI	ACCWGGT	1 cut(s)	1391
SfaNI	GCATC	2 cut(s)	132, 1456
SfcI	CTRYAG	1 cut(s)	205
SfoI	GGCGCC	1 cut(s)	930
Sfr274I	CTCGAG	1 cut(s)	293
SinI	GGWCC	6 cut(s)	253, 300, 400, 668, 965, 1208
SlaI	CTCGAG	1 cut(s)	293
SmiMI	CAYNNNNRTG	3 cut(s)	440, 1292, 1424
SmlI	CTYRAG	2 cut(s)	293, 1039
SmoI	CTYRAG	2 cut(s)	293, 1039
Sse9I	AATT	3 cut(s)	841, 1434, 1608
SseBI	AGGCCT	1 cut(s)	700
SspDI	GGCGCC	1 cut(s)	928
SspMI	CTAG	4 cut(s)	257, 873, 1305, 1790
SstI	GAGCTC	3 cut(s)	635, 1040, 1238
StuI	AGGCCT	1 cut(s)	700
StyD4I	CCNGG	4 cut(s)	495, 941, 1391, 1730
StyI	CCWWGG	4 cut(s)	549, 695, 795, 1695
TaaI	ACNGT	4 cut(s)	377, 492, 616, 1546
TaiI	ACGT	2 cut(s)	30, 57
TasI	AATT	3 cut(s)	841, 1434, 1608
TatI	WGTACW	2 cut(s)	459, 1651
TauI	GCSGC	5 cut(s)	49, 221, 974, 1300, 1604
TfiI	GAWTC	3 cut(s)	533, 1277, 1572
Tru1I	TTAA	2 cut(s)	689, 774
Tru9I	TTAA	2 cut(s)	689, 774
TscAI	CASTG	5 cut(s)	425, 619, 787, 943, 1585
TseFI	GTSAC	3 cut(s)	377, 610, 958
TseI	GCWGC	5 cut(s)	390, 899, 1115, 1598, 1658
Tsp45I	GTSAC	3 cut(s)	377, 610, 958
TspDTI	ATGAA	7 cut(s)	163, 202, 215, 1061, 1113, 1495, 1700
TspGWI	ACGGA	5 cut(s)	70, 570, 681, 1537, 1671
TspRI	CASTG	5 cut(s)	425, 619, 787, 943, 1585
VpaK11BI	GGWCC	6 cut(s)	253, 300, 400, 668, 965, 1208
XagI	CCTNNNNNAGG	1 cut(s)	362
XceI	RCATGY	2 cut(s)	373, 1027
XhoI	CTCGAG	1 cut(s)	293
XmnI	GAANNNNTTC	1 cut(s)	98
XspI	CTAG	4 cut(s)	257, 873, 1305, 1790

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.