pycom14g08280

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002064
Physical Location & Seq
Reverse (-)
8914037 .. 8916472
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g08280.1

Sequence Viewer

Length: 1797 bp
ATGATCAGTTTCACAGACCTGTACCACGTCCTCACCGCCGTTGTGCCGCTGTACGTGGCCATGATCTTAGCCTACGGCTCCGTGAAATGGTGGAAGATCTTCAGCCCCGACCAATGCTCCGGCATCAACCGCTTCGTCGCTCTGTTTGCAGTTCCTCTGCTCTCCTTCCACTTCATCTCCACCAACAACCCCTATGCCATGAACTACAGGTTCATAGCGGCTGACACGCTTCAGAAAGTCATCGTCCTGTTGGTCCTAGCCGTGTGGTCGAGAACCAGCGCCAGAGGCTCTCTCGAGTGGTCCATCACTCTTTTCTCCCTCTCCACTCTGCCCAACACTCTGGTCATGGGGATACCCCTTCTGAAGGGCATGTACGGTGACGCCTCCGGCACCCTCATGGTCCAAATCGTCGTCCTCCAGTGCATCATTTGGTACACTCTTATGCTGTTCCTCTTCGAGTACAGAGGCGCTCGCCTCCTCATTGGTGAACAGTTCCCGGACACCGCCGGCTCCATCATTTCCTTCAGGGTCGATTCGGACATCATTTCCTTGGACGGAAAAGAGCCGTTGCAGACAGAGGCCGAGGTCGGAGAAGACGGGAAGCTCCATGTCACAGTCAGAAAATCGACGAGCTCTCGCTCCGAAATATACTCCAGAAGATCCCACGGACCCAACTCCGGCGTGTCCTTAACCCCAAGGCCTTCTAATCTCACCAATGCTGAGATTTACAGCCTTCAGTCTTCGCGAAACCCGACTCCCAGAGGCTCGAGCTTTAACCACACTGATTTCTACTCCATGGTGAACGGGAAAAACATGAGCAATGCGAGTCCGAGGCAGTCGAATTACGGGAATGTGGGGTTTGATGAGGAGGCTAGAGGAGTTGGGGGGCCCAGAGGAGCAGCAAATGTGGGGAGTTTGGGGTATCCGGCGCCTCCCAGTGCCGGGATATTCTCTCCGGTGACCGGACCCGCCGCTGGGGCAAAGAAGCAACCCAATGGCACCGATGGTGGCAAAGACCTACACATGTTTGTTTGGAGCTCAAGCGCTTCGCCTGTGTCGGAAGGAGGCATTCATGTTTTCAGAGGAGGAGAATATGGCAATGAACTTGCTGGGGTTGCTGCTCTCCACAAAGATTATGATGAGTTTGGCCGAGATGAGTTCAGCTTCGACAACAGGCCGGCTTCAAATGGGGCTGATCGGGAAGGTCCGGTGCTTTCCAAGTTGGATTCGAGCTCCACCACTGAACTCCACCCCAAGACAGCCAATGGTGGTGAATCAAAGACCACAGCCATGCCGCCTGCTAGCGTTATGACCCGACTCATTCTTATTATGGTGTGGCGAAAACTGATTAGGAATCCAAACACTTACTCCAGCCTCATTGGCCTCACCTGGTCTCTGGTCTCCTACAAGTGGCAAATTGTTATGCCTGCAATTGTAGCCCGTTCGATAGCCATTCTGTCTGATGCAGGTCTTGGAATGGCCATGTTTAGTCTTGGCTTGTTCATGGCTTTACAACCACGGATGATTGCTTGCGGAAATACAGTTGCTTCCTTTGCTATGGCAGTTCGATTCCTCACTGGGCCGGCTGTCATGGCTGCGGCCTCAATTGCTGTTGGACTGCGAGGTGTTTTGTTGCACATTGCCATTGTACAGGCAGCTCTTCCACAAGGGATTGTTCCGTTTGTCTTTGCCAAGGAATACAATGTTCATCCTGACATACTAAGCACCGGGGTTATCTTCGGAATGCTAATCGCTCTTCCGATCACATTAGTTTACTACATCTTGCTAGGGCTTTGA

Protein Analysis

599

Amino Acids

64.52

Weight (kDa)

8.88

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 9 - 593 7.3e-125 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1457
AccB1I GGYRCC 3 cut(s) 389, 928, 998
AccII CGCG 1 cut(s) 745
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 3 cut(s) 57, 1147, 1479
AcuI CTGAAG 5 cut(s) 85, 215, 383, 508, 719
AcyI GRCGYC 2 cut(s) 381, 929
AfaI GTAC 6 cut(s) 23, 53, 374, 434, 461, 1650
AfeI AGCGCT 1 cut(s) 1045
AfiI CCNNNNNNNGG 9 cut(s) 87, 364, 677, 941, 942, 962, 974, 975, 1410
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 1 cut(s) 1185
AjiI CACGTC 1 cut(s) 28
AjnI CCWGG 1 cut(s) 1388
AluBI AGCT 7 cut(s) 604, 633, 771, 1038, 1164, 1233, 1658
AluI AGCT 7 cut(s) 604, 633, 771, 1038, 1164, 1233, 1658
Alw21I GWGCWC 3 cut(s) 635, 1040, 1235
Alw26I GTCTC 2 cut(s) 1398, 1405
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 2 cut(s) 293, 766
Aor51HI AGCGCT 1 cut(s) 1045
ApaI GGGCCC 1 cut(s) 891
ApeKI GCWGC 4 cut(s) 899, 1118, 1595, 1655
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 4 cut(s) 281, 470, 931, 1046
AspS9I GGNCC 9 cut(s) 253, 300, 400, 668, 887, 888, 965, 1205, 1580
AsuC2I CCSGG 3 cut(s) 497, 943, 1729
AsuHPI GGTGA 8 cut(s) 25, 389, 497, 703, 811, 970, 1283, 1378
AsuNHI GCTAGC 1 cut(s) 1301
AvaI CYCGRG 2 cut(s) 293, 766
AvaII GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
BaeGI GKGCMC 1 cut(s) 891
BalI TGGCCA 2 cut(s) 59, 1481
BanI GGYRCC 3 cut(s) 389, 928, 998
BanII GRGCYC 4 cut(s) 635, 891, 1040, 1235
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BbsI GAAGAC 2 cut(s) 600, 732
Bbv12I GWGCWC 3 cut(s) 635, 1040, 1235
BbvI GCAGC 4 cut(s) 911, 1105, 1582, 1667
BccI CCATC 3 cut(s) 311, 521, 998
BceAI ACGGC 4 cut(s) 23, 91, 245, 550
BciT130I CCWGG 1 cut(s) 1390
BciVI GTATCC 2 cut(s) 345, 933
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 3 cut(s) 497, 943, 1729
BcoDI GTCTC 2 cut(s) 1398, 1405
BfaI CTAG 4 cut(s) 257, 873, 1302, 1787
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 4 cut(s) 282, 471, 932, 1047
BfuAI ACCTGC 1 cut(s) 1457
BfuI GTATCC 2 cut(s) 345, 933
BglI GCCNNNNNGGC 2 cut(s) 977, 1380
BglII AGATCT 1 cut(s) 96
BisI GCNGC 9 cut(s) 47, 219, 900, 972, 1119, 1295, 1596, 1599, 1656
BlsI GCNGC 9 cut(s) 48, 220, 901, 973, 1120, 1296, 1597, 1600, 1657
Bme1390I CCNGG 4 cut(s) 497, 943, 1390, 1729
Bme18I GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
BmeT110I CYCGRG 2 cut(s) 293, 766
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 9 cut(s) 253, 300, 400, 668, 887, 888, 965, 1205, 1580
BmiI GGNNCC 9 cut(s) 79, 391, 511, 670, 888, 889, 930, 967, 1000
BmrFI CCNGG 4 cut(s) 497, 943, 1390, 1729
BmrI ACTGGG 2 cut(s) 930, 1587
BmsI GCATC 3 cut(s) 132, 432, 1453
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 2 cut(s) 930, 1587
BpiI GAAGAC 2 cut(s) 600, 732
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 3 cut(s) 401, 637, 1354
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 3 cut(s) 497, 943, 1729
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 2 cut(s) 381, 929
BsaI GGTCTC 2 cut(s) 1398, 1405
BsaJI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 830, 1517, 1692, 1728
BsaWI WCCGGW 3 cut(s) 955, 962, 1207
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 161, 191, 1352, 1382
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 364, 677, 941, 942, 962, 974, 975, 1410
Bse118I RCCGGY 3 cut(s) 506, 1177, 1582
Bse1I ACTGG 3 cut(s) 418, 936, 1582
Bse3DI GCAATG 3 cut(s) 826, 1105, 1638
BseBI CCWGG 1 cut(s) 1390
BseDI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 830, 1517, 1692, 1728
BseGI GGATG 2 cut(s) 1527, 1708
BseLI CCNNNNNNNGG 9 cut(s) 87, 364, 677, 941, 942, 962, 974, 975, 1410
BseMI GCAATG 3 cut(s) 826, 1105, 1638
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 3 cut(s) 418, 936, 1582
BseRI GAGGAG 6 cut(s) 467, 881, 891, 909, 1098, 1101
BseSI GKGCMC 1 cut(s) 891
BseXI GCAGC 4 cut(s) 911, 1105, 1582, 1667
BseYI CCCAGC 2 cut(s) 974, 1109
Bsh1236I CGCG 1 cut(s) 745
BshNI GGYRCC 3 cut(s) 389, 928, 998
BsiHKAI GWGCWC 3 cut(s) 635, 1040, 1235
BsiHKCI CYCGRG 2 cut(s) 293, 766
BslI CCNNNNNNNGG 9 cut(s) 87, 364, 677, 941, 942, 962, 974, 975, 1410
BsmAI GTCTC 2 cut(s) 1398, 1405
BsmI GAATGC 2 cut(s) 1068, 1749
Bso31I GGTCTC 2 cut(s) 1398, 1405
BsoBI CYCGRG 2 cut(s) 293, 766
Bsp120I GGGCCC 1 cut(s) 887
Bsp1286I GDGCHC 4 cut(s) 635, 891, 1040, 1235
Bsp1407I TGTACA 1 cut(s) 1648
Bsp143I GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
Bsp19I CCATGG 1 cut(s) 795
Bsp68I TCGCGA 1 cut(s) 745
BspCNI CTCAG 1 cut(s) 712
BspFNI CGCG 1 cut(s) 745
BspLI GGNNCC 9 cut(s) 79, 391, 511, 670, 888, 889, 930, 967, 1000
BspMI ACCTGC 1 cut(s) 1457
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 1 cut(s) 654
BspQI GCTCTTC 2 cut(s) 1665, 1761
BspT107I GGYRCC 3 cut(s) 389, 928, 998
BspTNI GGTCTC 2 cut(s) 1398, 1405
BsrDI GCAATG 3 cut(s) 826, 1105, 1638
BsrFI RCCGGY 3 cut(s) 506, 1177, 1582
BsrGI TGTACA 1 cut(s) 1648
BsrI ACTGG 3 cut(s) 418, 936, 1582
BssAI RCCGGY 3 cut(s) 506, 1177, 1582
BssECI CCNNGG 9 cut(s) 549, 582, 664, 695, 795, 830, 1517, 1692, 1728
BssMI GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
BssNI GRCGYC 2 cut(s) 381, 929
BssT1I CCWWGG 4 cut(s) 549, 695, 795, 1692
Bst2UI CCWGG 1 cut(s) 1390
Bst4CI ACNGT 4 cut(s) 377, 492, 616, 1543
Bst6I CTCTTC 3 cut(s) 458, 1665, 1761
BstACI GRCGYC 2 cut(s) 381, 929
BstAUI TGTACA 1 cut(s) 1648
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 8 cut(s) 472, 508, 1179, 1299, 1303, 1428, 1531, 1584
BstDEI CTNAG 3 cut(s) 67, 720, 1721
BstDSI CCRYGG 3 cut(s) 664, 795, 1517
BstEII GGTNACC 1 cut(s) 958
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 1527, 1708
BstFNI CGCG 1 cut(s) 745
BstH2I RGCGCY 4 cut(s) 282, 471, 932, 1047
BstHHI GCGC 4 cut(s) 281, 470, 931, 1046
BstKTI GATC 6 cut(s) 6, 66, 99, 662, 1198, 1764
BstMAI GTCTC 2 cut(s) 1398, 1405
BstMBI GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
BstNI CCWGG 1 cut(s) 1390
BstNSI RCATGY 2 cut(s) 373, 1027
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 4 cut(s) 495, 941, 1388, 1727
BstSFI CTRYAG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 891
BstUI CGCG 1 cut(s) 745
BstV1I GCAGC 4 cut(s) 911, 1105, 1582, 1667
BstV2I GAAGAC 2 cut(s) 600, 732
BstX2I RGATCY 2 cut(s) 96, 659
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 2 cut(s) 96, 659
BsuI GTATCC 2 cut(s) 345, 933
BtgI CCRYGG 3 cut(s) 664, 795, 1517
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 2 cut(s) 1527, 1708
BtsIMutI CAGTG 5 cut(s) 425, 780, 943, 1239, 1575
BtuMI TCGCGA 1 cut(s) 745
BveI ACCTGC 1 cut(s) 1457
Cac8I GCNNGC 8 cut(s) 472, 508, 1179, 1299, 1303, 1428, 1531, 1584
CfoI GCGC 4 cut(s) 281, 470, 931, 1046
Cfr10I RCCGGY 3 cut(s) 506, 1177, 1582
Cfr13I GGNCC 9 cut(s) 253, 300, 400, 668, 887, 888, 965, 1205, 1580
CseI GACGC 1 cut(s) 389
CsiI ACCWGGT 1 cut(s) 1388
Csp6I GTAC 6 cut(s) 22, 52, 373, 433, 460, 1649
CviQI GTAC 6 cut(s) 22, 52, 373, 433, 460, 1649
DdeI CTNAG 3 cut(s) 67, 720, 1721
DinI GGCGCC 1 cut(s) 930
DpnI GATC 6 cut(s) 5, 65, 98, 661, 1197, 1763
DpnII GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
EaeI YGGCCR 3 cut(s) 57, 1147, 1479
Eam1104I CTCTTC 3 cut(s) 458, 1665, 1761
EarI CTCTTC 3 cut(s) 458, 1665, 1761
Ecl136II GAGCTC 3 cut(s) 633, 1038, 1233
Eco130I CCWWGG 4 cut(s) 549, 695, 795, 1692
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 4 cut(s) 635, 891, 1040, 1235
Eco31I GGTCTC 2 cut(s) 1398, 1405
Eco47I GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
Eco47III AGCGCT 1 cut(s) 1045
Eco53kI GAGCTC 3 cut(s) 633, 1038, 1233
Eco57I CTGAAG 5 cut(s) 85, 215, 383, 508, 719
Eco88I CYCGRG 2 cut(s) 293, 766
Eco91I GGTNACC 1 cut(s) 958
EcoICRI GAGCTC 3 cut(s) 633, 1038, 1233
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 887
EcoO65I GGTNACC 1 cut(s) 958
EcoRII CCWGG 1 cut(s) 1388
EcoT14I CCWWGG 4 cut(s) 549, 695, 795, 1692
EcoT38I GRGCYC 4 cut(s) 635, 891, 1040, 1235
EgeI GGCGCC 1 cut(s) 930
EheI GGCGCC 1 cut(s) 930
ErhI CCWWGG 4 cut(s) 549, 695, 795, 1692
FauI CCCGC 1 cut(s) 976
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 9 cut(s) 47, 219, 900, 972, 1119, 1295, 1596, 1599, 1656
FokI GGATG 2 cut(s) 1534, 1695
FriOI GRGCYC 4 cut(s) 635, 891, 1040, 1235
Fsp4HI GCNGC 9 cut(s) 47, 219, 900, 972, 1119, 1295, 1596, 1599, 1656
FspBI CTAG 4 cut(s) 257, 873, 1302, 1787
GlaI GCGC 4 cut(s) 280, 469, 930, 1045
GluI GCNGC 9 cut(s) 47, 219, 900, 972, 1119, 1295, 1596, 1599, 1656
GsaI CCCAGC 2 cut(s) 978, 1113
GsuI CTGGAG 3 cut(s) 401, 637, 1354
HaeII RGCGCY 4 cut(s) 282, 471, 932, 1047
HgaI GACGC 1 cut(s) 389
HhaI GCGC 4 cut(s) 281, 470, 931, 1046
Hin1I GRCGYC 2 cut(s) 381, 929
Hin6I GCGC 4 cut(s) 279, 468, 929, 1044
HinP1I GCGC 4 cut(s) 279, 468, 929, 1044
HinfI GANTC 8 cut(s) 533, 754, 826, 1226, 1274, 1317, 1354, 1569
HphI GGTGA 8 cut(s) 25, 389, 497, 703, 811, 970, 1283, 1378
Hpy166II GTNNAC 4 cut(s) 435, 488, 802, 1774
Hpy188III TCNNGA 6 cut(s) 270, 293, 654, 744, 1199, 1712
Hpy8I GTNNAC 4 cut(s) 435, 488, 802, 1774
Hpy99I CGWCG 3 cut(s) 140, 413, 631
HpyAV CCTTC 8 cut(s) 175, 358, 368, 532, 711, 743, 1055, 1196
HpyCH4III ACNGT 4 cut(s) 377, 492, 616, 1543
HpyCH4IV ACGT 2 cut(s) 27, 54
HpyCH4V TGCA 6 cut(s) 149, 423, 571, 1430, 1466, 1636
HpyF3I CTNAG 3 cut(s) 67, 720, 1721
HpySE526I ACGT 2 cut(s) 27, 54
Hsp92I GRCGYC 2 cut(s) 381, 929
HspAI GCGC 4 cut(s) 279, 468, 929, 1044
KasI GGCGCC 1 cut(s) 928
KroI GCCGGC 3 cut(s) 506, 1177, 1582
KroNI GCCGGC 3 cut(s) 508, 1179, 1584
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
LguI GCTCTTC 2 cut(s) 1665, 1761
LmnI GCTCC 8 cut(s) 83, 122, 515, 609, 644, 896, 1035, 1238
Lsp1109I GCAGC 4 cut(s) 911, 1105, 1582, 1667
LweI GCATC 3 cut(s) 132, 432, 1453
MabI ACCWGGT 1 cut(s) 1388
MaeI CTAG 4 cut(s) 257, 873, 1302, 1787
MaeII ACGT 2 cut(s) 27, 54
MaeIII GTNAC 3 cut(s) 377, 610, 958
MalI GATC 6 cut(s) 5, 65, 98, 661, 1197, 1763
MboI GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
MboII GAAGA 9 cut(s) 91, 106, 445, 605, 669, 732, 1652, 1729, 1748
MfeI CAATTG 2 cut(s) 1431, 1605
MflI RGATCY 2 cut(s) 96, 659
MhlI GDGCHC 4 cut(s) 635, 891, 1040, 1235
MlsI TGGCCA 2 cut(s) 59, 1481
MluCI AATT 4 cut(s) 841, 1416, 1431, 1605
MluNI TGGCCA 2 cut(s) 59, 1481
Mly113I GGCGCC 1 cut(s) 929
MlyI GAGTC 3 cut(s) 748, 835, 1311
MmeI TCCRAC 4 cut(s) 568, 1038, 1203, 1594
Mox20I TGGCCA 2 cut(s) 59, 1481
MroNI GCCGGC 3 cut(s) 506, 1177, 1582
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 2 cut(s) 59, 1481
MseI TTAA 2 cut(s) 689, 774
MslI CAYNNNNRTG 3 cut(s) 395, 440, 1289
Msp20I TGGCCA 2 cut(s) 59, 1481
MspA1I CMGCKG 2 cut(s) 49, 974
MspR9I CCNGG 4 cut(s) 497, 943, 1390, 1729
MunI CAATTG 2 cut(s) 1431, 1605
Mva1269I GAATGC 2 cut(s) 1068, 1749
MvaI CCWGG 1 cut(s) 1390
MvnI CGCG 1 cut(s) 745
NaeI GCCGGC 3 cut(s) 508, 1179, 1584
NarI GGCGCC 1 cut(s) 929
NciI CCSGG 3 cut(s) 497, 943, 1729
NcoI CCATGG 1 cut(s) 795
NdeII GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
NgoMIV GCCGGC 3 cut(s) 506, 1177, 1582
NheI GCTAGC 1 cut(s) 1301
NlaIV GGNNCC 9 cut(s) 79, 391, 511, 670, 888, 889, 930, 967, 1000
NmeAIII GCCGAG 2 cut(s) 607, 1175
NmuCI GTSAC 3 cut(s) 377, 610, 958
NruI TCGCGA 1 cut(s) 745
NspI RCATGY 2 cut(s) 373, 1027
PaeR7I CTCGAG 2 cut(s) 293, 766
PceI AGGCCT 1 cut(s) 700
PciI ACATGT 1 cut(s) 1023
PciSI GCTCTTC 2 cut(s) 1665, 1761
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PctI GAATGC 2 cut(s) 1068, 1749
PdiI GCCGGC 3 cut(s) 508, 1179, 1584
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 5 cut(s) 533, 1226, 1274, 1354, 1569
PfoI TCCNGGA 1 cut(s) 495
PkrI GCNGC 9 cut(s) 48, 220, 901, 973, 1120, 1296, 1597, 1600, 1657
PleI GAGTC 3 cut(s) 748, 834, 1311
PluTI GGCGCC 1 cut(s) 932
PpsI GAGTC 3 cut(s) 748, 834, 1311
Ppu21I YACGTR 1 cut(s) 55
PscI ACATGT 1 cut(s) 1023
Psp124BI GAGCTC 3 cut(s) 635, 1040, 1235
Psp6I CCWGG 1 cut(s) 1388
PspEI GGTNACC 1 cut(s) 958
PspFI CCCAGC 2 cut(s) 974, 1109
PspGI CCWGG 1 cut(s) 1388
PspN4I GGNNCC 9 cut(s) 79, 391, 511, 670, 888, 889, 930, 967, 1000
PspOMI GGGCCC 1 cut(s) 887
PspPI GGNCC 9 cut(s) 253, 300, 400, 668, 887, 888, 965, 1205, 1580
PspXI VCTCGAGB 1 cut(s) 766
PsuI RGATCY 2 cut(s) 96, 659
RruI TCGCGA 1 cut(s) 745
RsaI GTAC 6 cut(s) 23, 53, 374, 434, 461, 1650
RsaNI GTAC 6 cut(s) 22, 52, 373, 433, 460, 1649
RseI CAYNNNNRTG 3 cut(s) 395, 440, 1289
SacI GAGCTC 3 cut(s) 635, 1040, 1235
SapI GCTCTTC 2 cut(s) 1665, 1761
SaqAI TTAA 2 cut(s) 689, 774
SatI GCNGC 9 cut(s) 47, 219, 900, 972, 1119, 1295, 1596, 1599, 1656
Sau3AI GATC 6 cut(s) 3, 63, 96, 659, 1195, 1761
Sau96I GGNCC 9 cut(s) 253, 300, 400, 668, 887, 888, 965, 1205, 1580
SchI GAGTC 3 cut(s) 748, 835, 1311
ScrFI CCNGG 4 cut(s) 497, 943, 1390, 1729
SduI GDGCHC 4 cut(s) 635, 891, 1040, 1235
SexAI ACCWGGT 1 cut(s) 1388
SfaNI GCATC 3 cut(s) 132, 432, 1453
SfcI CTRYAG 1 cut(s) 205
SfoI GGCGCC 1 cut(s) 930
Sfr274I CTCGAG 2 cut(s) 293, 766
SinI GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
SlaI CTCGAG 2 cut(s) 293, 766
SmiMI CAYNNNNRTG 3 cut(s) 395, 440, 1289
SmlI CTYRAG 3 cut(s) 293, 766, 1039
SmoI CTYRAG 3 cut(s) 293, 766, 1039
Sse9I AATT 4 cut(s) 841, 1416, 1431, 1605
SseBI AGGCCT 1 cut(s) 700
SspDI GGCGCC 1 cut(s) 928
SspMI CTAG 4 cut(s) 257, 873, 1302, 1787
SstI GAGCTC 3 cut(s) 635, 1040, 1235
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 4 cut(s) 495, 941, 1388, 1727
StyI CCWWGG 4 cut(s) 549, 695, 795, 1692
TaaI ACNGT 4 cut(s) 377, 492, 616, 1543
TaiI ACGT 2 cut(s) 30, 57
TasI AATT 4 cut(s) 841, 1416, 1431, 1605
TatI WGTACW 2 cut(s) 459, 1648
TauI GCSGC 5 cut(s) 49, 221, 974, 1297, 1601
TfiI GAWTC 5 cut(s) 533, 1226, 1274, 1354, 1569
Tru1I TTAA 2 cut(s) 689, 774
Tru9I TTAA 2 cut(s) 689, 774
TscAI CASTG 5 cut(s) 425, 787, 943, 1246, 1582
TseFI GTSAC 3 cut(s) 377, 610, 958
TseI GCWGC 4 cut(s) 899, 1118, 1595, 1655
Tsp45I GTSAC 3 cut(s) 377, 610, 958
TspDTI ATGAA 7 cut(s) 163, 202, 215, 1061, 1116, 1492, 1697
TspGWI ACGGA 5 cut(s) 70, 570, 681, 1534, 1668
TspRI CASTG 5 cut(s) 425, 787, 943, 1246, 1582
VpaK11BI GGWCC 6 cut(s) 253, 300, 400, 668, 965, 1205
XagI CCTNNNNNAGG 1 cut(s) 362
XceI RCATGY 2 cut(s) 373, 1027
XhoI CTCGAG 2 cut(s) 293, 766
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 4 cut(s) 257, 873, 1302, 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.