Rroxscaffold_3G00274900

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002064
Physical Location & Seq
Forward (+)
66517794 .. 66521334
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274900.1

Sequence Viewer

Length: 858 bp
ATGTTCTCGCCTACCACGGCGGCCTCTAAGAACGTTGTCGCTCCCAAGAGGCCTAATGGAAATGGGCCTAATGGGCCGGCGGCTCAGGACGGCGGTAGGGATCTTCATATGTTTGTCTGGAGCTCCAGTGCTTCTCCTGTTTCTGATGTGTTTGGTAGCAATGAATATGGGGCCGCTGGTCATGATCAGAAAGAAGTCAAATTGGCCGTTTCTCCGGGAAAAGTGGAGGGTAGAAGAGAGAACCAAGAAGAGTACATGGTGGAGAGAGATGAGTTCAGCTTTGGGAACAGAGTCGTGGATCAGATGAACATGAATGAGGGAGAGAAAGTGGATGTGAATAAGGCCAAAGTGATGCCTCCGACGAGTGTCATGACGAGGCTGATTCTCATCATGGTCTGGAGAAAATTGATCAGAAACCCAAACACTTACTCAAGCTTGATCGGCCTCACTTGGTCTCTGGTATCCTTCAGGTGGCATGTAGAAATGCCAGCAATCATAGCGAAGTCGATAGCCATTCTATCGGACGCAGGGCTTGGGATGGCCATGTTCAGTCTTGGTCTGTTTATGGCTTTACAACCAAGGATCATAGCATGTGGAAATTCCATTGCAGCTTTTACTATGGCTGTGAGATTCCTTACAGGTCCAGCTGTCATGGCAGCTGCTTCCATTGCTGTTGGCCTTAGGGGCACTCTCTTACATGTTGCTATTGTCCAGGCAGCTCTACCCCAAGGAATTGTCCCCTTTGTCTTTGCCAAGGAATACAACGTACACCCTGATATTCTCAGCACGGGGGTTATATTCGGCATGTTGATCGCGTTGCCCATTACGCTTGTCTACTACATAATGCTGGGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009505 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009965 GO:0009966 GO:0009987 GO:0010016 GO:0010033 GO:0010051 GO:0010154 GO:0010229 GO:0010252 GO:0010315 GO:0010329 GO:0010338 GO:0010358 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016021 GO:0016323 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0023052 GO:0030054 GO:0030312 GO:0031224 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045177 GO:0045178 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048532 GO:0048583 GO:0048598 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048830 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055044 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0080161 GO:0090567 GO:0090698 GO:0098590 GO:0098796 GO:0098797 GO:0099402 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

285

Amino Acids

30.8

Weight (kDa)

8.99

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 90 - 280 3.2e-62 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 834
AccII CGCG 1 cut(s) 815
AciI CCGC 4 cut(s) 20, 80, 93, 174
AclI AACGTT 1 cut(s) 33
AclWI GGATC 3 cut(s) 108, 306, 590
AcoI YGGCCR 2 cut(s) 204, 540
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 451
AfaI GTAC 2 cut(s) 254, 768
AfiI CCNNNNNNNGG 1 cut(s) 471
AflIII ACRYGT 1 cut(s) 697
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 7 cut(s) 123, 279, 435, 611, 647, 659, 719
AluI AGCT 7 cut(s) 123, 279, 435, 611, 647, 659, 719
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 3 cut(s) 108, 306, 590
ApeKI GCWGC 4 cut(s) 608, 656, 659, 716
ApoI RAATTY 1 cut(s) 598
AspS9I GGNCC 4 cut(s) 65, 74, 171, 641
AsuC2I CCSGG 1 cut(s) 216
AvaII GGWCC 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 1 cut(s) 542
BanII GRGCYC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 4 cut(s) 620, 646, 668, 728
BccI CCATC 1 cut(s) 532
BceAI ACGGC 3 cut(s) 33, 106, 191
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 1 cut(s) 713
BciVI GTATCC 1 cut(s) 472
BclI TGATCA 2 cut(s) 184, 408
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 459
BfuI GTATCC 1 cut(s) 472
BglI GCCNNNNNGGC 2 cut(s) 73, 684
BisI GCNGC 7 cut(s) 21, 81, 174, 609, 657, 660, 717
BlsI GCNGC 7 cut(s) 22, 82, 175, 610, 658, 661, 718
Bme1390I CCNGG 2 cut(s) 216, 713
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 4 cut(s) 65, 74, 171, 641
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 2 cut(s) 216, 713
BmsI GCATC 1 cut(s) 342
BoxI GACNNNNGTC 1 cut(s) 365
BpmI CTGGAG 3 cut(s) 109, 139, 418
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 459
BsaJI CCNNGG 4 cut(s) 15, 578, 727, 753
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 312, 342
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 126
Bse21I CCTNAGG 1 cut(s) 680
Bse3DI GCAATG 3 cut(s) 166, 603, 666
Bse8I GATNNNNATC 1 cut(s) 386
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 4 cut(s) 15, 578, 727, 753
BseGI GGATG 2 cut(s) 337, 543
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMI GCAATG 3 cut(s) 166, 603, 666
BseMII CTCAG 2 cut(s) 98, 796
BseNI ACTGG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 4 cut(s) 620, 646, 668, 728
BseYI CCCAGC 1 cut(s) 847
Bsh1236I CGCG 1 cut(s) 815
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 2 cut(s) 77, 215
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 722
Bso31I GGTCTC 1 cut(s) 459
Bsp1286I GDGCHC 2 cut(s) 125, 689
Bsp143I GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
BspACI CCGC 4 cut(s) 20, 80, 93, 174
BspCNI CTCAG 2 cut(s) 97, 795
BspFNI CGCG 1 cut(s) 815
BspHI TCATGA 2 cut(s) 181, 369
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 3 cut(s) 108, 306, 590
BspTNI GGTCTC 1 cut(s) 459
BsrDI GCAATG 3 cut(s) 166, 603, 666
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 4 cut(s) 15, 578, 727, 753
BssMI GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
BssT1I CCWWGG 3 cut(s) 578, 727, 753
Bst2UI CCWGG 1 cut(s) 713
Bst6I CTCTTC 2 cut(s) 229, 243
BstC8I GCNNGC 2 cut(s) 78, 489
BstDEI CTNAG 4 cut(s) 27, 84, 680, 782
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 337, 543
BstFNI CGCG 1 cut(s) 815
BstKTI GATC 7 cut(s) 103, 187, 301, 411, 441, 585, 813
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
BstMWI GCNNNNNNNGC 7 cut(s) 73, 441, 497, 653, 668, 684, 826
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 4 cut(s) 479, 594, 701, 808
BstPAI GACNNNNGTC 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 214, 711
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 1 cut(s) 815
BstV1I GCAGC 4 cut(s) 620, 646, 668, 728
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu36I CCTNAGG 1 cut(s) 680
BsuI GTATCC 1 cut(s) 472
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 2 cut(s) 337, 543
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 78, 489
CciI TCATGA 2 cut(s) 181, 369
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 4 cut(s) 65, 74, 171, 641
CseI GACGC 1 cut(s) 533
Csp6I GTAC 2 cut(s) 253, 767
CviQI GTAC 2 cut(s) 253, 767
DdeI CTNAG 4 cut(s) 27, 84, 680, 782
DpnI GATC 7 cut(s) 102, 186, 300, 410, 440, 584, 812
DpnII GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
EaeI YGGCCR 2 cut(s) 204, 540
Eam1104I CTCTTC 2 cut(s) 229, 243
EarI CTCTTC 2 cut(s) 229, 243
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 3 cut(s) 578, 727, 753
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 641
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 451
Eco81I CCTNAGG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 3 cut(s) 578, 727, 753
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 3 cut(s) 578, 727, 753
FaqI GGGAC 1 cut(s) 722
FauNDI CATATG 1 cut(s) 108
FbaI TGATCA 2 cut(s) 184, 408
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 7 cut(s) 21, 81, 174, 609, 657, 660, 717
FokI GGATG 2 cut(s) 344, 550
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 7 cut(s) 21, 81, 174, 609, 657, 660, 717
GluI GCNGC 7 cut(s) 21, 81, 174, 609, 657, 660, 717
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 3 cut(s) 109, 139, 418
HapII CCGG 2 cut(s) 77, 215
HgaI GACGC 1 cut(s) 533
HindIII AAGCTT 1 cut(s) 433
HinfI GANTC 3 cut(s) 291, 382, 630
HpaII CCGG 2 cut(s) 77, 215
Hpy166II GTNNAC 2 cut(s) 769, 835
Hpy188I TCNGA 6 cut(s) 145, 189, 303, 360, 413, 523
Hpy188III TCNNGA 5 cut(s) 86, 118, 182, 370, 397
Hpy8I GTNNAC 2 cut(s) 769, 835
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 475
HpyCH4IV ACGT 2 cut(s) 33, 765
HpyCH4V TGCA 1 cut(s) 608
HpyF10VI GCNNNNNNNGC 7 cut(s) 73, 441, 497, 653, 668, 684, 826
HpyF3I CTNAG 4 cut(s) 27, 84, 680, 782
HpySE526I ACGT 2 cut(s) 33, 765
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 184, 408
Kzo9I GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
LmnI GCTCC 3 cut(s) 46, 120, 128
Lsp1109I GCAGC 4 cut(s) 620, 646, 668, 728
LweI GCATC 1 cut(s) 342
MaeII ACGT 2 cut(s) 33, 765
MalI GATC 7 cut(s) 102, 186, 300, 410, 440, 584, 812
MboI GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
MboII GAAGA 3 cut(s) 95, 246, 260
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 125, 689
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 4 cut(s) 200, 404, 598, 732
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 7 cut(s) 34, 42, 220, 310, 366, 369, 455
Mox20I TGGCCA 1 cut(s) 542
MroNI GCCGGC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 542
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 3 cut(s) 176, 647, 659
MspI CCGG 2 cut(s) 77, 215
MspR9I CCNGG 2 cut(s) 216, 713
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 1 cut(s) 815
MwoI GCNNNNNNNGC 7 cut(s) 73, 441, 497, 653, 668, 684, 826
NaeI GCCGGC 1 cut(s) 78
NciI CCSGG 1 cut(s) 216
NdeI CATATG 1 cut(s) 108
NdeII GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
NgoMIV GCCGGC 1 cut(s) 76
NlaIV GGNNCC 1 cut(s) 172
NspI RCATGY 4 cut(s) 479, 594, 701, 808
PagI TCATGA 2 cut(s) 181, 369
PceI AGGCCT 1 cut(s) 52
PciI ACATGT 1 cut(s) 697
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 382, 630
PfoI TCCNGGA 1 cut(s) 214
PkrI GCNGC 7 cut(s) 22, 82, 175, 610, 658, 661, 718
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PscI ACATGT 1 cut(s) 697
PshAI GACNNNNGTC 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 125
Psp1406I AACGTT 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 847
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 4 cut(s) 65, 74, 171, 641
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 2 cut(s) 647, 659
RsaI GTAC 2 cut(s) 254, 768
RsaNI GTAC 2 cut(s) 253, 767
SacI GAGCTC 1 cut(s) 125
SatI GCNGC 7 cut(s) 21, 81, 174, 609, 657, 660, 717
Sau3AI GATC 7 cut(s) 100, 184, 298, 408, 438, 582, 810
Sau96I GGNCC 4 cut(s) 65, 74, 171, 641
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 2 cut(s) 216, 713
SduI GDGCHC 2 cut(s) 125, 689
SfaNI GCATC 1 cut(s) 342
SfiI GGCCNNNNNGGCC 1 cut(s) 73
SinI GGWCC 1 cut(s) 641
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 4 cut(s) 200, 404, 598, 732
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 4 cut(s) 20, 80, 93, 174
SstI GAGCTC 1 cut(s) 125
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 2 cut(s) 214, 711
StyI CCWWGG 3 cut(s) 578, 727, 753
TaiI ACGT 2 cut(s) 36, 768
TaqI TCGA 1 cut(s) 506
TasI AATT 4 cut(s) 200, 404, 598, 732
TatI WGTACW 1 cut(s) 252
TauI GCSGC 3 cut(s) 23, 83, 176
TfiI GAWTC 2 cut(s) 382, 630
TscAI CASTG 1 cut(s) 133
TseI GCWGC 4 cut(s) 608, 656, 659, 716
TspDTI ATGAA 4 cut(s) 95, 177, 320, 326
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 1 cut(s) 598
XceI RCATGY 4 cut(s) 479, 594, 701, 808
XmiI GTMKAC 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.