Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005614

Physical Location & Seq

Genomic Coordinates Forward (+)

17757407 .. 17766342

Gene Structure 8936 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1112000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGCCTTCCTTTCCTCATCCCGGTTCTGTCACCATTTGCGAAATCAATCGCGACCTCATCACTGCTGAGAGCCTCTCGGACGATGTAGCCAAGGACACTTATGGAAAGATTCTTGGAATGGTATTCAGCCCACTTCCGTTTCAATCGGATCAGCTGGTGCAGCCCCCGAGTCCCGACCATGATGCCGAGCAGGTTGTAACCACATCTCCAACAAAGGGATATCTAAAAAATTTGCAGGGGATTATTAACCTCACTCCTCTCTTCCGCCCTAATCATGTAAATTTATTACCAGAAGTTGATCTTCAAGGAGTGAGCTGGCACCAACATAAACATGTCGTAGCGCTTATATCTGGCCCCAATCAAGTTATAATTCGTGATTTCGAGGATTTAGGGAAGGAGCCATGCATTTTGGCCAATGAATCCCAGAGAGAGGTCAAAGTACTTGAGTGGAGGCCTAACGGTGGGAGACAACTTGCTGTAGCATGCAAGCAAACAGGCTGTAAGTTAGGTGGAATTTGCATTTGGGCTGCTTCTTTCCCGGGAAGTGCAGCGTCTATCAGATCTGGGTCTTCTTCCTTCCTGGGCACTCTCTCCAGAGGTTCTGGAATTCGATATACTTTGGTGGATTTTCTTCGAACTCATAATGAGGAACAGATTAGTGCGTTGTCATGGAGTCCTGATGGAAGATATCCAAACAATCTCTATTCTGATTTTCAATTGTGGTTAAACTTTGGAGACTCTACATTACATGAATATGGAGGTTTTCCTGCAATTTATCACTGTCATCCATATCTGCAAACTTTATCAAATGTTTTGACAAAACTTTATATTTTTGTTTTCCTTTTGCATTGTATTGCCGTTTTTCACAAAAAAATTACTTTCTCTACAGTATTATGTTGTGGCATCAATTTTCATTTTATACATAGGGATATTCTCAAGCAGGAAAAGAGCCTTTATTTTCAGATTAATTTCACACTTTGTTCAGTCAGCTCATCATTTACCATATGGGATGTTTCTCAAGGGCTTGGGACACCTATTAGACGAGGACTAGGTGGCATTTCAATGCTGAAGTGGTCGCCAACTGGGGATTACTTTTTTTCGGCAAAATTTGATGGAACATTTTATCTTTGGGAGACAAACACATGGACATCTGAACCATGGTCTTCAACTAGTGGCTTCGTCAAGGGAGCATCATGGGATCCTGATGGGCGTATGATACTTCTCGCTTTCTCTAAATCTTCAACCATGGGTTCAATTCACTTTGCTTCAAAGCCTCCATCACTTGATGCGCACTTATTACCTGTTGAACTACCAGAGATGATGTCCTTAACAAATAGTCAAGGCATTGAGAAGATAGCTTGGGATGCTTCAGGAGAGCGACTGGCTATATCTTTTAAAGATGGGGGTGACTTATACCGGGGTCTTATTGCCATTTACGATGTTAGGAGGACCCCTCTAATATCTGCATCATTGATTGGGTTTATTAGAGGTCCCGGAGACAATCCAAAACCAGTGGCATTTTCATTTCATGACAAGTTTAAACAGGGACCATTGCTTTTTGTGGTAAGTCCGAGTCCTAGTACTCAAATGTTGTGTGCTTTTGCTTTACCCCATGGTACTTGGAACCCTTGTCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003008 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006810 GO:0006913 GO:0007610 GO:0007611 GO:0007612 GO:0008150 GO:0009566 GO:0012505 GO:0015630 GO:0016020 GO:0019953 GO:0022414 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046907 GO:0050877 GO:0050890 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

546

Amino Acids

60.41

Weight (kDa)

6.8

Isoelectric Point (pI)

37.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Aladin	PF25460	101 - 184	4e-07	Aladin seven-bladed propeller
Beta-prop_Aladin	PF25460	327 - 527	1.9e-31	Aladin seven-bladed propeller
Beta-prop_THOC3	PF25174	327 - 467	6.6e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005614)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56900
fragaria_vesca	FvH4_7g04320 FvH4_7g04320 FvH4_7g04320 FvH4_7g04320
malus_domestica	MD12G1112000.v1.1 MD14G1101000.v1.1 MD14G1101100.v1.1
prunus_persica	Prupe.7G021600_v2.0.a1 Prupe.7G021600_v2.0.a1 Prupe.7G021600_v2.0.a1 Prupe.7G021600_v2.0.a1
pyrus_communis	pycom12g09160 pycom14g08800
rosa_chinensis	RchiOBHm_Chr1g0329271 RchiOBHm_Chr1g0329281
rosa_laevigata	RLG00000029905
rosa_multiflora	Rmu_co8376675.1_g000001 Rmu_sc0000203.1_g000006 Rmu_sc0010272.1_g000001
rosa_rugosa	Rorug01G0077700
rosa_samantha	Rh1AG096600 Rh1BG077300 Rh1CG093300 Rh1DG099600 Rh1DG100000
rosa_wichuraiana	Rw1G007480 Rw1G007560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	368
Acc16I	TGCGCA	1 cut(s)	1290
Acc36I	ACCTGC	1 cut(s)	181
AccB1I	GGYRCC	1 cut(s)	318
AccII	CGCG	1 cut(s)	51
AciI	CCGC	1 cut(s)	265
AclWI	GGATC	3 cut(s)	156, 1193, 1206
AcoI	YGGCCR	1 cut(s)	411
AcsI	RAATTY	5 cut(s)	229, 280, 513, 606, 1106
AcuI	CTGAAG	2 cut(s)	1088, 1353
AfaI	GTAC	3 cut(s)	441, 1582, 1618
AfeI	AGCGCT	1 cut(s)	342
AfiI	CCNNNNNNNGG	4 cut(s)	20, 215, 430, 461
AflIII	ACRYGT	1 cut(s)	331
AgsI	TTSAA	9 cut(s)	143, 305, 716, 1062, 1167, 1242, 1254, 1269, 1307
AhlI	ACTAGT	1 cut(s)	1169
AjnI	CCWGG	1 cut(s)	579
AjuI	GAANNNNNNNTTGG	2 cut(s)	505, 537
AluBI	AGCT	4 cut(s)	154, 315, 990, 1358
AluI	AGCT	4 cut(s)	154, 315, 990, 1358
Alw26I	GTCTC	4 cut(s)	460, 729, 1127, 1491
AlwI	GGATC	3 cut(s)	156, 1193, 1206
Ama87I	CYCGRG	2 cut(s)	166, 538
Aor51HI	AGCGCT	1 cut(s)	342
AoxI	GGCC	3 cut(s)	352, 411, 452
ApeKI	GCWGC	3 cut(s)	160, 527, 548
ApoI	RAATTY	5 cut(s)	229, 280, 513, 606, 1106
AseI	ATTAAT	1 cut(s)	966
AspLEI	GCGC	2 cut(s)	343, 1291
AspS9I	GGNCC	4 cut(s)	353, 1449, 1490, 1547
AsuC2I	CCSGG	5 cut(s)	21, 539, 540, 1418, 1494
AsuHPI	GGTGA	2 cut(s)	22, 1418
AsuII	TTCGAA	1 cut(s)	634
AvaI	CYCGRG	2 cut(s)	166, 538
AvaII	GGWCC	3 cut(s)	1449, 1490, 1547
BaeGI	GKGCMC	1 cut(s)	587
BalI	TGGCCA	1 cut(s)	413
BamHI	GGATCC	1 cut(s)	1198
BanI	GGYRCC	1 cut(s)	318
BbsI	GAAGAC	2 cut(s)	561, 1155
BbvI	GCAGC	3 cut(s)	172, 514, 560
BccI	CCATC	5 cut(s)	674, 1106, 1199, 1285, 1394
BceAI	ACGGC	1 cut(s)	842
BcgI	CGANNNNNNTGC	2 cut(s)	164, 198
BciT130I	CCWGG	1 cut(s)	581
BcnI	CCSGG	5 cut(s)	21, 539, 540, 1418, 1494
BcoDI	GTCTC	4 cut(s)	460, 729, 1127, 1491
BcuI	ACTAGT	1 cut(s)	1169
BfaI	CTAG	3 cut(s)	1049, 1170, 1578
BfmI	CTRYAG	2 cut(s)	477, 885
BfoI	RGCGCY	1 cut(s)	344
BfuAI	ACCTGC	1 cut(s)	181
BglII	AGATCT	1 cut(s)	560
BisI	GCNGC	3 cut(s)	161, 528, 549
BlsI	GCNGC	3 cut(s)	162, 529, 550
BmcAI	AGTACT	2 cut(s)	441, 1582
Bme1390I	CCNGG	6 cut(s)	21, 539, 540, 581, 1418, 1494
Bme18I	GGWCC	3 cut(s)	1449, 1490, 1547
BmeT110I	CYCGRG	2 cut(s)	166, 538
BmgT120I	GGNCC	4 cut(s)	353, 1449, 1490, 1547
BmiI	GGNNCC	8 cut(s)	320, 355, 399, 1200, 1451, 1492, 1548, 1625
BmrFI	CCNGG	6 cut(s)	21, 539, 540, 581, 1418, 1494
BmrI	ACTGGG	1 cut(s)	1092
BmsI	GCATC	6 cut(s)	172, 912, 1199, 1276, 1354, 1475
BmuI	ACTGGG	1 cut(s)	1092
BpiI	GAAGAC	2 cut(s)	561, 1155
BplI	GAGNNNNNCTC	4 cut(s)	59, 91, 1438, 1470
BpmI	CTGGAG	1 cut(s)	577
Bpu14I	TTCGAA	1 cut(s)	634
BpuEI	CTTGAG	3 cut(s)	464, 920, 1002
BpuMI	CCSGG	5 cut(s)	21, 539, 540, 1418, 1494
BsaJI	CCNNGG	7 cut(s)	90, 538, 580, 1157, 1245, 1417, 1612
BsaXI	ACNNNNNCTCC	2 cut(s)	190, 220
Bsc4I	CCNNNNNNNGG	4 cut(s)	20, 215, 430, 461
Bse1I	ACTGG	3 cut(s)	1087, 1386, 1511
Bse3DI	GCAATG	1 cut(s)	1550
BseBI	CCWGG	1 cut(s)	581
BseDI	CCNNGG	7 cut(s)	90, 538, 580, 1157, 1245, 1417, 1612
BseGI	GGATG	4 cut(s)	16, 784, 1015, 1369
BseLI	CCNNNNNNNGG	4 cut(s)	20, 215, 430, 461
BseMI	GCAATG	1 cut(s)	1550
BseMII	CTCAG	1 cut(s)	57
BseNI	ACTGG	3 cut(s)	1087, 1386, 1511
BseRI	GAGGAG	1 cut(s)	246
BseSI	GKGCMC	1 cut(s)	587
BseXI	GCAGC	3 cut(s)	172, 514, 560
BsgI	GTGCAG	2 cut(s)	179, 567
Bsh1236I	CGCG	1 cut(s)	51
BshFI	GGCC	3 cut(s)	354, 413, 454
BshNI	GGYRCC	1 cut(s)	318
BsiHKCI	CYCGRG	2 cut(s)	166, 538
BsiSI	CCGG	4 cut(s)	21, 539, 1417, 1494
BslFI	GGGAC	4 cut(s)	156, 1042, 1476, 1560
BslI	CCNNNNNNNGG	4 cut(s)	20, 215, 430, 461
BsmAI	GTCTC	4 cut(s)	460, 729, 1127, 1491
BsmFI	GGGAC	4 cut(s)	156, 1042, 1476, 1560
BsnI	GGCC	3 cut(s)	354, 413, 454
BsoBI	CYCGRG	2 cut(s)	166, 538
Bsp119I	TTCGAA	1 cut(s)	634
Bsp1286I	GDGCHC	1 cut(s)	587
Bsp143I	GATC	4 cut(s)	148, 298, 560, 1198
Bsp19I	CCATGG	3 cut(s)	1157, 1245, 1612
Bsp68I	TCGCGA	1 cut(s)	51
BspACI	CCGC	1 cut(s)	265
BspANI	GGCC	3 cut(s)	354, 413, 454
BspCNI	CTCAG	1 cut(s)	58
BspFNI	CGCG	1 cut(s)	51
BspHI	TCATGA	1 cut(s)	1528
BspLI	GGNNCC	8 cut(s)	320, 355, 399, 1200, 1451, 1492, 1548, 1625
BspMI	ACCTGC	1 cut(s)	181
BspPI	GGATC	3 cut(s)	156, 1193, 1206
BspT104I	TTCGAA	1 cut(s)	634
BspT107I	GGYRCC	1 cut(s)	318
BsrDI	GCAATG	1 cut(s)	1550
BsrI	ACTGG	3 cut(s)	1087, 1386, 1511
BssECI	CCNNGG	7 cut(s)	90, 538, 580, 1157, 1245, 1417, 1612
BssMI	GATC	4 cut(s)	148, 298, 560, 1198
BssT1I	CCWWGG	4 cut(s)	90, 1157, 1245, 1612
Bst2UI	CCWGG	1 cut(s)	581
Bst4CI	ACNGT	3 cut(s)	461, 782, 889
Bst6I	CTCTTC	1 cut(s)	266
BstBI	TTCGAA	1 cut(s)	634
BstC8I	GCNNGC	3 cut(s)	317, 484, 488
BstDEI	CTNAG	1 cut(s)	66
BstDSI	CCRYGG	3 cut(s)	1157, 1245, 1612
BstF5I	GGATG	4 cut(s)	16, 784, 1015, 1369
BstFNI	CGCG	1 cut(s)	51
BstH2I	RGCGCY	1 cut(s)	344
BstHHI	GCGC	2 cut(s)	343, 1291
BstKTI	GATC	4 cut(s)	151, 301, 563, 1201
BstMAI	GTCTC	4 cut(s)	460, 729, 1127, 1491
BstMBI	GATC	4 cut(s)	148, 298, 560, 1198
BstMWI	GCNNNNNNNGC	2 cut(s)	160, 1364
BstNI	CCWGG	1 cut(s)	581
BstNSI	RCATGY	2 cut(s)	335, 486
BstSCI	CCNGG	6 cut(s)	19, 537, 538, 579, 1416, 1492
BstSFI	CTRYAG	2 cut(s)	477, 885
BstSLI	GKGCMC	1 cut(s)	587
BstUI	CGCG	1 cut(s)	51
BstV1I	GCAGC	3 cut(s)	172, 514, 560
BstV2I	GAAGAC	2 cut(s)	561, 1155
BstX2I	RGATCY	2 cut(s)	560, 1198
BstYI	RGATCY	2 cut(s)	560, 1198
BsuRI	GGCC	3 cut(s)	354, 413, 454
BtgI	CCRYGG	3 cut(s)	1157, 1245, 1612
BtsCI	GGATG	4 cut(s)	16, 784, 1015, 1369
BtsI	GCAGTG	1 cut(s)	60
BtsIMutI	CAGTG	3 cut(s)	60, 778, 1518
BtuMI	TCGCGA	1 cut(s)	51
BveI	ACCTGC	1 cut(s)	181
Cac8I	GCNNGC	3 cut(s)	317, 484, 488
CciI	TCATGA	1 cut(s)	1528
CfoI	GCGC	2 cut(s)	343, 1291
Cfr13I	GGNCC	4 cut(s)	353, 1449, 1490, 1547
Cfr9I	CCCGGG	1 cut(s)	538
CseI	GACGC	1 cut(s)	540
Csp6I	GTAC	3 cut(s)	440, 1581, 1617
CspCI	CAANNNNNGTGG	2 cut(s)	1494, 1529
CviQI	GTAC	3 cut(s)	440, 1581, 1617
DdeI	CTNAG	1 cut(s)	66
DpnI	GATC	4 cut(s)	150, 300, 562, 1200
DpnII	GATC	4 cut(s)	148, 298, 560, 1198
DraI	TTTAAA	2 cut(s)	1396, 1540
EaeI	YGGCCR	1 cut(s)	411
Eam1104I	CTCTTC	1 cut(s)	266
EarI	CTCTTC	1 cut(s)	266
EciI	GGCGGA	1 cut(s)	254
Eco130I	CCWWGG	4 cut(s)	90, 1157, 1245, 1612
Eco147I	AGGCCT	1 cut(s)	454
Eco32I	GATATC	2 cut(s)	221, 689
Eco47I	GGWCC	3 cut(s)	1449, 1490, 1547
Eco47III	AGCGCT	1 cut(s)	342
Eco57I	CTGAAG	2 cut(s)	1088, 1353
Eco88I	CYCGRG	2 cut(s)	166, 538
EcoO109I	RGGNCCY	2 cut(s)	1449, 1490
EcoRI	GAATTC	1 cut(s)	606
EcoRII	CCWGG	1 cut(s)	579
EcoRV	GATATC	2 cut(s)	221, 689
EcoT14I	CCWWGG	4 cut(s)	90, 1157, 1245, 1612
EcoT22I	ATGCAT	1 cut(s)	407
ErhI	CCWWGG	4 cut(s)	90, 1157, 1245, 1612
FalI	AAGNNNNNCTT	2 cut(s)	285, 317
FaqI	GGGAC	4 cut(s)	156, 1042, 1476, 1560
FauNDI	CATATG	1 cut(s)	1004
Fnu4HI	GCNGC	3 cut(s)	161, 528, 549
FokI	GGATG	4 cut(s)	3, 771, 1022, 1376
Fsp4HI	GCNGC	3 cut(s)	161, 528, 549
FspAI	RTGCGCAY	1 cut(s)	1290
FspBI	CTAG	3 cut(s)	1049, 1170, 1578
FspI	TGCGCA	1 cut(s)	1290
GlaI	GCGC	2 cut(s)	342, 1290
GluI	GCNGC	3 cut(s)	161, 528, 549
GsuI	CTGGAG	1 cut(s)	577
HaeII	RGCGCY	1 cut(s)	344
HaeIII	GGCC	3 cut(s)	354, 413, 454
HapII	CCGG	4 cut(s)	21, 539, 1417, 1494
HgaI	GACGC	1 cut(s)	540
HhaI	GCGC	2 cut(s)	343, 1291
Hin6I	GCGC	2 cut(s)	341, 1289
HinP1I	GCGC	2 cut(s)	341, 1289
HinfI	GANTC	6 cut(s)	109, 169, 419, 673, 737, 1573
HpaII	CCGG	4 cut(s)	21, 539, 1417, 1494
HphI	GGTGA	2 cut(s)	22, 1418
Hpy188I	TCNGA	7 cut(s)	79, 148, 560, 709, 963, 1153, 1572
Hpy188III	TCNNGA	9 cut(s)	50, 173, 374, 594, 603, 677, 1202, 1371, 1529
HpyAV	CCTTC	3 cut(s)	15, 388, 586
HpyCH4III	ACNGT	3 cut(s)	461, 782, 889
HpyF10VI	GCNNNNNNNGC	2 cut(s)	160, 1364
HpyF3I	CTNAG	1 cut(s)	66
HspAI	GCGC	2 cut(s)	341, 1289
Kzo9I	GATC	4 cut(s)	148, 298, 560, 1198
LmnI	GCTCC	2 cut(s)	397, 1187
Lsp1109I	GCAGC	3 cut(s)	172, 514, 560
LweI	GCATC	6 cut(s)	172, 912, 1199, 1276, 1354, 1475
MaeI	CTAG	3 cut(s)	1049, 1170, 1578
MaeIII	GTNAC	3 cut(s)	28, 196, 1406
MalI	GATC	4 cut(s)	150, 300, 562, 1200
MboI	GATC	4 cut(s)	148, 298, 560, 1198
MboII	GAAGA	9 cut(s)	253, 293, 561, 564, 623, 696, 1155, 1230, 1363
MfeI	CAATTG	1 cut(s)	716
MflI	RGATCY	2 cut(s)	560, 1198
MhlI	GDGCHC	1 cut(s)	587
MlsI	TGGCCA	1 cut(s)	413
MluNI	TGGCCA	1 cut(s)	413
MlyI	GAGTC	4 cut(s)	178, 682, 731, 1582
MmeI	TCCRAC	1 cut(s)	233
Mox20I	TGGCCA	1 cut(s)	413
Mph1103I	ATGCAT	1 cut(s)	407
MscI	TGGCCA	1 cut(s)	413
MseI	TTAA	6 cut(s)	246, 725, 966, 1328, 1395, 1539
MslI	CAYNNNNRTG	3 cut(s)	330, 753, 1061
Msp20I	TGGCCA	1 cut(s)	413
MspA1I	CMGCKG	1 cut(s)	154
MspI	CCGG	4 cut(s)	21, 539, 1417, 1494
MspR9I	CCNGG	6 cut(s)	21, 539, 540, 581, 1418, 1494
MssI	GTTTAAAC	1 cut(s)	1540
MunI	CAATTG	1 cut(s)	716
MvaI	CCWGG	1 cut(s)	581
MvnI	CGCG	1 cut(s)	51
MwoI	GCNNNNNNNGC	2 cut(s)	160, 1364
NciI	CCSGG	5 cut(s)	21, 539, 540, 1418, 1494
NcoI	CCATGG	3 cut(s)	1157, 1245, 1612
NdeI	CATATG	1 cut(s)	1004
NdeII	GATC	4 cut(s)	148, 298, 560, 1198
NlaIV	GGNNCC	8 cut(s)	320, 355, 399, 1200, 1451, 1492, 1548, 1625
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	2 cut(s)	28, 1406
NruI	TCGCGA	1 cut(s)	51
NsbI	TGCGCA	1 cut(s)	1290
NsiI	ATGCAT	1 cut(s)	407
NspI	RCATGY	2 cut(s)	335, 486
NspV	TTCGAA	1 cut(s)	634
PaeI	GCATGC	1 cut(s)	486
PagI	TCATGA	1 cut(s)	1528
PceI	AGGCCT	1 cut(s)	454
PciI	ACATGT	1 cut(s)	331
PfeI	GAWTC	2 cut(s)	109, 419
PfoI	TCCNGGA	1 cut(s)	1492
PkrI	GCNGC	3 cut(s)	162, 529, 550
PleI	GAGTC	4 cut(s)	177, 681, 731, 1581
PmeI	GTTTAAAC	1 cut(s)	1540
PpsI	GAGTC	4 cut(s)	177, 681, 731, 1581
PpuMI	RGGWCCY	2 cut(s)	1449, 1490
PscI	ACATGT	1 cut(s)	331
PshBI	ATTAAT	1 cut(s)	966
PsiI	TTATAA	1 cut(s)	368
Psp5II	RGGWCCY	2 cut(s)	1449, 1490
Psp6I	CCWGG	1 cut(s)	579
PspGI	CCWGG	1 cut(s)	579
PspN4I	GGNNCC	8 cut(s)	320, 355, 399, 1200, 1451, 1492, 1548, 1625
PspPI	GGNCC	4 cut(s)	353, 1449, 1490, 1547
PspPPI	RGGWCCY	2 cut(s)	1449, 1490
PsuI	RGATCY	2 cut(s)	560, 1198
PvuII	CAGCTG	1 cut(s)	154
RruI	TCGCGA	1 cut(s)	51
RsaI	GTAC	3 cut(s)	441, 1582, 1618
RsaNI	GTAC	3 cut(s)	440, 1581, 1617
RseI	CAYNNNNRTG	3 cut(s)	330, 753, 1061
SaqAI	TTAA	6 cut(s)	246, 725, 966, 1328, 1395, 1539
SatI	GCNGC	3 cut(s)	161, 528, 549
Sau3AI	GATC	4 cut(s)	148, 298, 560, 1198
Sau96I	GGNCC	4 cut(s)	353, 1449, 1490, 1547
ScaI	AGTACT	2 cut(s)	441, 1582
SchI	GAGTC	4 cut(s)	178, 682, 731, 1582
ScrFI	CCNGG	6 cut(s)	21, 539, 540, 581, 1418, 1494
SduI	GDGCHC	1 cut(s)	587
SfaNI	GCATC	6 cut(s)	172, 912, 1199, 1276, 1354, 1475
SfcI	CTRYAG	2 cut(s)	477, 885
SfuI	TTCGAA	1 cut(s)	634
SinI	GGWCC	3 cut(s)	1449, 1490, 1547
SmaI	CCCGGG	1 cut(s)	540
SmiMI	CAYNNNNRTG	3 cut(s)	330, 753, 1061
SmlI	CTYRAG	3 cut(s)	443, 935, 1017
SmoI	CTYRAG	3 cut(s)	443, 935, 1017
SpeI	ACTAGT	1 cut(s)	1169
SphI	GCATGC	1 cut(s)	486
SseBI	AGGCCT	1 cut(s)	454
SsiI	CCGC	1 cut(s)	265
SspMI	CTAG	3 cut(s)	1049, 1170, 1578
StuI	AGGCCT	1 cut(s)	454
StyD4I	CCNGG	6 cut(s)	19, 537, 538, 579, 1416, 1492
StyI	CCWWGG	4 cut(s)	90, 1157, 1245, 1612
TaaI	ACNGT	3 cut(s)	461, 782, 889
TaqI	TCGA	3 cut(s)	381, 610, 634
TatI	WGTACW	2 cut(s)	439, 1580
TfiI	GAWTC	2 cut(s)	109, 419
Tru1I	TTAA	6 cut(s)	246, 725, 966, 1328, 1395, 1539
Tru9I	TTAA	6 cut(s)	246, 725, 966, 1328, 1395, 1539
TscAI	CASTG	3 cut(s)	67, 785, 1518
TseFI	GTSAC	2 cut(s)	28, 1406
TseI	GCWGC	3 cut(s)	160, 527, 548
Tsp45I	GTSAC	2 cut(s)	28, 1406
TspDTI	ATGAA	5 cut(s)	432, 765, 902, 1512, 1517
TspGWI	ACGGA	1 cut(s)	126
TspMI	CCCGGG	1 cut(s)	538
TspRI	CASTG	3 cut(s)	67, 785, 1518
VpaK11BI	GGWCC	3 cut(s)	1449, 1490, 1547
VspI	ATTAAT	1 cut(s)	966
XapI	RAATTY	5 cut(s)	229, 280, 513, 606, 1106
XceI	RCATGY	2 cut(s)	335, 486
XmaI	CCCGGG	1 cut(s)	538
XspI	CTAG	3 cut(s)	1049, 1170, 1578
ZrmI	AGTACT	2 cut(s)	441, 1582
Zsp2I	ATGCAT	1 cut(s)	407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.