MD12G1115500.v1.1

Translin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005043
Physical Location & Seq
Forward (+)
18432026 .. 18434947
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1115500.v1.1.491

Sequence Viewer

Length: 894 bp
ATGAAGTCAGCGTTTCGAAACGCCCACTCTCTCTTCCGCTCCCTAAACCCTAACCCTAACCCTCACCTCACCTCGCTTCTCCTCCCTCTCCGTCTCACACTCCTCACCCCACCCTCACCTCCGTCCCTCAGACCCCAAACATTTCCCTCCCTCTCCGCCACTCTCTCCTCCGCGTCGATGCCTGGCGGCGAACCCCTCACGCCTCCCCCGCTGGAGAAGCAGTTCGAGGACTTCCGAGCTCAGCTTGAAGAGTCCGGGACCTTGCGCGAGCGCATTCGAGCTGTGGTTATGGAGATCGAGTCCACCACAAGGCTAATGCACTCCGGCCTCCTCCTAGTTCACCAGTCTCGCTCCACTCCCGAGGTTTTGGAGAAGCCGAAGGTACAGATTGGCGTGTTGAAGGAGCTCTACAATCGGCTCGCTGAAGTCGTGCGTGAATCTCCTGGGCAGTACTATAGATATCATGGTGACTGGAGGACTGAGACGCAGACTGTGGTTTCACTGCTTGCGTTTATGCACTGGTTGGAGACAGGGACTCTTTTGTTGCACACTGAAGCTGAAGAAAAACTTGGACTGAACGATTCAGAATTTTGTCTGGATGTTGAAGACTATCTTGTTGGTATTTGTTTCATGTCCAACGAATTGCCGAGGTACGTGGTTAACCAAGTGACTGCTGGGGACTATGACTGTCCAAGAAAGGTGCTTAAGTTTTTGACAGATCTTCATGCAGCCTTCCGTATGCTTAATCTCCGGAATGACTTTTTGCGCAAGAAATTTGATGGTATGAAGTATGACCTGAGAAGGGTTGAAGAAGTTTACTACGATGTTAAGATCAGAGGCTTGACAGCCAATGGTGCTCTTGTGGAAGACCAAGGACCCCAAGGACCGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Translin

Protein Analysis

298

Amino Acids

33.79

Weight (kDa)

6.27

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Translin PF01997 88 - 280 7.3e-58 Translin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 767
AccBSI CCGCTC 1 cut(s) 39
AccII CGCG 2 cut(s) 173, 267
AccIII TCCGGA 1 cut(s) 750
AciI CCGC 6 cut(s) 37, 156, 171, 186, 209, 887
AcsI RAATTY 2 cut(s) 587, 773
AcuI CTGAAG 3 cut(s) 444, 573, 579
AfaI GTAC 3 cut(s) 384, 452, 653
AfiI CCNNNNNNNGG 2 cut(s) 309, 802
AflII CTTAAG 1 cut(s) 704
AgsI TTSAA 4 cut(s) 248, 400, 605, 809
AjnI CCWGG 2 cut(s) 181, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
AluBI AGCT 5 cut(s) 239, 244, 281, 406, 557
AluI AGCT 5 cut(s) 239, 244, 281, 406, 557
Alw21I GWGCWC 3 cut(s) 241, 408, 859
Alw26I GTCTC 4 cut(s) 98, 351, 476, 521
Ama87I CYCGRG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 750
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 2 cut(s) 587, 773
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 3 cut(s) 267, 273, 768
AspS9I GGNCC 3 cut(s) 258, 875, 884
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 6 cut(s) 56, 61, 97, 108, 332, 479
AsuII TTCGAA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 3 cut(s) 258, 875, 884
BanII GRGCYC 2 cut(s) 241, 408
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
BbsI GAAGAC 2 cut(s) 612, 873
Bbv12I GWGCWC 3 cut(s) 241, 408, 859
BbvI GCAGC 1 cut(s) 740
BccI CCATC 1 cut(s) 773
BciT130I CCWGG 2 cut(s) 183, 444
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 4 cut(s) 98, 351, 476, 521
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 454
BfrI CTTAAG 1 cut(s) 704
BglII AGATCT 1 cut(s) 718
BisI GCNGC 2 cut(s) 187, 729
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 2 cut(s) 188, 730
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 3 cut(s) 183, 256, 444
Bme18I GGWCC 3 cut(s) 258, 875, 884
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 3 cut(s) 258, 875, 884
BmiI GGNNCC 2 cut(s) 259, 877
BmrFI CCNGG 3 cut(s) 183, 256, 444
BmsI GCATC 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 612, 873
BpmI CTGGAG 2 cut(s) 233, 493
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 256
BsaAI YACGTR 1 cut(s) 655
BsaJI CCNNGG 5 cut(s) 360, 443, 647, 871, 880
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 802
Bse1I ACTGG 3 cut(s) 343, 476, 524
BseAI TCCGGA 1 cut(s) 750
BseBI CCWGG 2 cut(s) 183, 444
BseDI CCNNGG 5 cut(s) 360, 443, 647, 871, 880
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 2 cut(s) 309, 802
BseMII CTCAG 4 cut(s) 142, 254, 471, 788
BseNI ACTGG 3 cut(s) 343, 476, 524
BseRI GAGGAG 4 cut(s) 71, 92, 157, 320
BseXI GCAGC 1 cut(s) 740
BseYI CCCAGC 1 cut(s) 674
Bsh1236I CGCG 2 cut(s) 173, 267
BshFI GGCC 1 cut(s) 327
BsiHKAI GWGCWC 3 cut(s) 241, 408, 859
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 3 cut(s) 255, 324, 751
BslFI GGGAC 4 cut(s) 109, 271, 547, 692
BslI CCNNNNNNNGG 2 cut(s) 309, 802
BsmAI GTCTC 4 cut(s) 98, 351, 476, 521
BsmBI CGTCTC 2 cut(s) 98, 476
BsmFI GGGAC 4 cut(s) 109, 271, 547, 692
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 3 cut(s) 241, 408, 859
Bsp13I TCCGGA 1 cut(s) 750
Bsp143I GATC 3 cut(s) 294, 718, 831
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 6 cut(s) 37, 156, 171, 186, 209, 887
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 4 cut(s) 141, 253, 472, 789
BspEI TCCGGA 1 cut(s) 750
BspFNI CGCG 2 cut(s) 173, 267
BspLI GGNNCC 2 cut(s) 259, 877
BspT104I TTCGAA 1 cut(s) 16
BspTI CTTAAG 1 cut(s) 704
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 3 cut(s) 343, 476, 524
BssECI CCNNGG 5 cut(s) 360, 443, 647, 871, 880
BssMI GATC 3 cut(s) 294, 718, 831
BssT1I CCWWGG 2 cut(s) 871, 880
Bst2UI CCWGG 2 cut(s) 183, 444
Bst4CI ACNGT 2 cut(s) 493, 689
Bst6I CTCTTC 2 cut(s) 38, 243
BstAFI CTTAAG 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 655
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 3 cut(s) 269, 420, 507
BstDEI CTNAG 4 cut(s) 128, 240, 480, 797
BstF5I GGATG 1 cut(s) 604
BstFNI CGCG 2 cut(s) 173, 267
BstHHI GCGC 3 cut(s) 267, 273, 768
BstKTI GATC 3 cut(s) 297, 721, 834
BstMAI GTCTC 4 cut(s) 98, 351, 476, 521
BstMBI GATC 3 cut(s) 294, 718, 831
BstMWI GCNNNNNNNGC 3 cut(s) 208, 217, 854
BstNI CCWGG 2 cut(s) 183, 444
BstSCI CCNGG 3 cut(s) 181, 254, 442
BstSFI CTRYAG 1 cut(s) 454
BstUI CGCG 2 cut(s) 173, 267
BstV1I GCAGC 1 cut(s) 740
BstV2I GAAGAC 2 cut(s) 612, 873
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BsuRI GGCC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 604
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 3 cut(s) 500, 517, 549
Cac8I GCNNGC 3 cut(s) 269, 420, 507
CfoI GCGC 3 cut(s) 267, 273, 768
Cfr13I GGNCC 3 cut(s) 258, 875, 884
CseI GACGC 2 cut(s) 162, 493
Csp6I GTAC 3 cut(s) 383, 451, 652
CviAII CATG 3 cut(s) 464, 631, 725
CviQI GTAC 3 cut(s) 383, 451, 652
DdeI CTNAG 4 cut(s) 128, 240, 480, 797
DpnI GATC 3 cut(s) 296, 720, 833
DpnII GATC 3 cut(s) 294, 718, 831
Eam1104I CTCTTC 2 cut(s) 38, 243
EarI CTCTTC 2 cut(s) 38, 243
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 2 cut(s) 239, 406
Eco130I CCWWGG 2 cut(s) 871, 880
Eco24I GRGCYC 2 cut(s) 241, 408
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 258, 875, 884
Eco53kI GAGCTC 2 cut(s) 239, 406
Eco57I CTGAAG 3 cut(s) 444, 573, 579
Eco88I CYCGRG 1 cut(s) 359
EcoICRI GAGCTC 2 cut(s) 239, 406
EcoO109I RGGNCCY 2 cut(s) 258, 875
EcoRII CCWGG 2 cut(s) 181, 442
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 2 cut(s) 871, 880
EcoT38I GRGCYC 2 cut(s) 241, 408
ErhI CCWWGG 2 cut(s) 871, 880
Esp3I CGTCTC 2 cut(s) 98, 476
FaeI CATG 3 cut(s) 467, 634, 728
FalI AAGNNNNNCTT 4 cut(s) 552, 584, 597, 629
FaqI GGGAC 4 cut(s) 109, 271, 547, 692
FatI CATG 3 cut(s) 463, 630, 724
FauI CCCGC 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 187, 729
FokI GGATG 1 cut(s) 611
FriOI GRGCYC 2 cut(s) 241, 408
Fsp4HI GCNGC 2 cut(s) 187, 729
FspBI CTAG 1 cut(s) 335
FspI TGCGCA 1 cut(s) 767
GlaI GCGC 3 cut(s) 266, 272, 767
GluI GCNGC 2 cut(s) 187, 729
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 2 cut(s) 233, 493
HaeIII GGCC 1 cut(s) 327
HapII CCGG 3 cut(s) 255, 324, 751
HgaI GACGC 2 cut(s) 162, 493
HhaI GCGC 3 cut(s) 267, 273, 768
Hin1II CATG 3 cut(s) 467, 634, 728
Hin6I GCGC 3 cut(s) 265, 271, 766
HinP1I GCGC 3 cut(s) 265, 271, 766
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 5 cut(s) 251, 299, 437, 535, 581
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 3 cut(s) 255, 324, 751
HphI GGTGA 6 cut(s) 56, 61, 97, 108, 332, 479
Hpy166II GTNNAC 4 cut(s) 303, 340, 661, 817
Hpy188I TCNGA 4 cut(s) 131, 236, 586, 836
Hpy188III TCNNGA 3 cut(s) 359, 596, 751
Hpy8I GTNNAC 4 cut(s) 303, 340, 661, 817
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 4 cut(s) 373, 394, 742, 795
HpyCH4III ACNGT 2 cut(s) 493, 689
HpyCH4IV ACGT 1 cut(s) 654
HpyCH4V TGCA 4 cut(s) 319, 517, 547, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 217, 854
HpyF3I CTNAG 4 cut(s) 128, 240, 480, 797
HpySE526I ACGT 1 cut(s) 654
Hsp92II CATG 3 cut(s) 467, 634, 728
HspAI GCGC 3 cut(s) 265, 271, 766
Kpn2I TCCGGA 1 cut(s) 750
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 3 cut(s) 294, 718, 831
LmnI GCTCC 3 cut(s) 44, 356, 403
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 1 cut(s) 168
MaeI CTAG 1 cut(s) 335
MaeII ACGT 1 cut(s) 654
MaeIII GTNAC 2 cut(s) 467, 667
MalI GATC 3 cut(s) 296, 720, 833
MbiI CCGCTC 1 cut(s) 39
MboI GATC 3 cut(s) 294, 718, 831
MboII GAAGA 7 cut(s) 25, 260, 572, 617, 713, 821, 878
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 3 cut(s) 241, 408, 859
MluCI AATT 3 cut(s) 587, 641, 773
MlyI GAGTC 3 cut(s) 260, 308, 529
MmeI TCCRAC 2 cut(s) 504, 660
MroI TCCGGA 1 cut(s) 750
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 4 cut(s) 660, 705, 744, 828
MspA1I CMGCKG 1 cut(s) 211
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 3 cut(s) 255, 324, 751
MspR9I CCNGG 3 cut(s) 183, 256, 444
Mva1269I GAATGC 1 cut(s) 273
MvaI CCWGG 2 cut(s) 183, 444
MvnI CGCG 2 cut(s) 173, 267
MwoI GCNNNNNNNGC 3 cut(s) 208, 217, 854
NciI CCSGG 1 cut(s) 256
NdeII GATC 3 cut(s) 294, 718, 831
NlaIII CATG 3 cut(s) 467, 634, 728
NlaIV GGNNCC 2 cut(s) 259, 877
NmeAIII GCCGAG 1 cut(s) 672
NmuCI GTSAC 2 cut(s) 467, 667
NsbI TGCGCA 1 cut(s) 767
NspV TTCGAA 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PctI GAATGC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 437, 581
PfoI TCCNGGA 1 cut(s) 254
PkrI GCNGC 2 cut(s) 188, 730
PleI GAGTC 3 cut(s) 259, 307, 529
PpsI GAGTC 3 cut(s) 259, 307, 529
Ppu21I YACGTR 1 cut(s) 655
PpuMI RGGWCCY 2 cut(s) 258, 875
Psp124BI GAGCTC 2 cut(s) 241, 408
Psp5II RGGWCCY 2 cut(s) 258, 875
Psp6I CCWGG 2 cut(s) 181, 442
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 181, 442
PspN4I GGNNCC 2 cut(s) 259, 877
PspPI GGNCC 3 cut(s) 258, 875, 884
PspPPI RGGWCCY 2 cut(s) 258, 875
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 3 cut(s) 384, 452, 653
RsaNI GTAC 3 cut(s) 383, 451, 652
SacI GAGCTC 2 cut(s) 241, 408
SaqAI TTAA 4 cut(s) 660, 705, 744, 828
SatI GCNGC 2 cut(s) 187, 729
Sau3AI GATC 3 cut(s) 294, 718, 831
Sau96I GGNCC 3 cut(s) 258, 875, 884
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 3 cut(s) 260, 308, 529
ScrFI CCNGG 3 cut(s) 183, 256, 444
SduI GDGCHC 3 cut(s) 241, 408, 859
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 454
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 3 cut(s) 258, 875, 884
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
Sse9I AATT 3 cut(s) 587, 641, 773
SsiI CCGC 6 cut(s) 37, 156, 171, 186, 209, 887
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 2 cut(s) 241, 408
StyD4I CCNGG 3 cut(s) 181, 254, 442
StyI CCWWGG 2 cut(s) 871, 880
TaaI ACNGT 2 cut(s) 493, 689
TaiI ACGT 1 cut(s) 657
TaqI TCGA 5 cut(s) 16, 176, 225, 277, 297
TasI AATT 3 cut(s) 587, 641, 773
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 2 cut(s) 437, 581
Tru1I TTAA 4 cut(s) 660, 705, 744, 828
Tru9I TTAA 4 cut(s) 660, 705, 744, 828
TscAI CASTG 3 cut(s) 507, 524, 556
TseFI GTSAC 2 cut(s) 467, 667
TseI GCWGC 1 cut(s) 728
Tsp45I GTSAC 2 cut(s) 467, 667
TspDTI ATGAA 4 cut(s) 17, 619, 713, 800
TspGWI ACGGA 3 cut(s) 80, 111, 725
TspRI CASTG 3 cut(s) 507, 524, 556
Vha464I CTTAAG 1 cut(s) 704
VpaK11BI GGWCC 3 cut(s) 258, 875, 884
XapI RAATTY 2 cut(s) 587, 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 1 cut(s) 335
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.