MD12G1154200.v1.1

proton-transporting ATP synthase activity, rotational mechanism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003626
Physical Location & Seq
Reverse (-)
23470922 .. 23481590
10669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1154200.v1.1.491

Sequence Viewer

Length: 4758 bp
ATGTTAGGAACTAGAGACCGCAGCCAAAATATCCCTTTAATTAATTACCTCACCAGAGTAATGCATGACGACCTCAAGACCGCAGCAAAAAGTGTCAATCATTGGTCTGGTGTCACCACACTGATTCCACTGTTTGGAGGCTTCGTGGCTGATGCTTACTTGGGGCACTTCAAAATAGTGTTTTTTTTCATCCATCATTTACCTCATGGGACTGGGGGTCATAAGCCTGCCCTGGAGAGCTTTGGAGCTGACCAATTCGATGACGATCACCGTGAAGAAAGAAAGCAGAAAATGTCCTTTTTCAATTGGTGGAGTTTTGGGCTTTGTTGTGGGCTTTTACTTGGGGTGACTGTGGTTGTGTATGTGCAAGACCGTGGGAGTTGGGGTGTTGCTGATGTTGTTGTCACTGCAGTCATGGCTGTATCACTTGCTATCTTCATAATTGGAAGGCGGAATTACCGTTATCGAAAACCGACTGGGAGCCCCTTTACACCAATGTTGCAAGTTCTTGTAGCTGCCATTGCTAAGAGAAATCAGCCTCACGCTTCTGACCCTGCTCAATTGTATGAAACCCCCAAATCAGAAAAGGTCCATGGGAGGCTTCTGTGCCACACGAGGAAGCTCAGATTTCTTGACAAGGCGGTGATCATAAGCGCACAAAACTTGGCTGAGAATCCAAGCCCTTGGAGACTAGCAACTGTGACCAAGGTGGAGAAGATGAAATTTGTTCTCAACTTGATCCCAATTTGGCTAGCGACTCTACCATTTGGAGTGTGTGTGGCACAATCCTCCACATACTTCATCAAACAAGGTGCCACCATGAACAGAGAGGTTGCAAATGGTTTCGAGGTCCCCCCTGCCTCAATCTTCGCACTCGCAGCCATTGGAATTATCGCCTCCGTCACGATATACGAAAAACTCCTCGTCCCGATTCTGAGAAGGACAACCGGAAACGAAAGAGGAATCAACATCCTCCAAAGGATAGGAATCAGAATGATTTTCTCCATCGCTACGATGGTAGCAGCTGCCCTGGTGGAGAAGAAACTACTAAGGTTTGTCAAAAGTGATCCAATAAAGGGTTTGTTAGACAATAGAATCCTATTAAACGGAGATAACTACACGACCTGGGCACGCGGGATGCACATCTCCTTAAGCGCCAAGAATAAACTTGGTTTTATCACAGGTGATATTGAAGAACCCTCACCTTCCAAGGACCCTGATGCTCACGCCTCTTGGAGGCGCTGCAATGATATGATCCTTTCATGGATCCTTCATTCCCTGGAGCCCGATCTTGCCGAATCAGTTCTTTTTTCCACCACCGCTAAGGCTGTTTGGGACGACCTTCATGAACGTTTTTTCCAGTCTAATGCTCCCCGTATCTTCCACCTGAATCGTGAGATTGCCACCATCTCCCAAGGGTCCTCCTCGGTCTCAACATACTTCACTCGCCTAAAAGGTCTTTGGGACGAGCTCGCCTCCTATAGTGATGCATACTCTTGCTCTTGTGGCACCAAACATGATCGTCAACAGCTTATGCAGTTTCTTATGGGTCTCAATGAATCGTTTGTCACCGTTCGGGACCAAATTCTTCTAATGAATCCTTTGCCCACCGTCCGCCAGGCCTGTTCATCTGTTTCTCAAGCTGAAAAACAACGTGCCCTTGGTTTTTCACGTACTGCCGAGCCTCCCGCTGCCATTGTTGTTCGTAGCTCTCCTCGGTCTTCGTCCGATCGGCCTCCTCTACATTGCACCTATTGTGGTTATGACTTCCACACCCGCGATACTTGCCATAAGCTTCATGGGTATCCGGTTGGCCATCCTCTCCACGGTCAGCCGTGGAGACCTCCTCGCCGATCCACCACTTCCGCAGGTTCTACCCGCAGCAGCGTTACTGCTCCTTCTGCTCGCAGCCATACTGCTACCTCTCGGTCCATCCACGGCTCAGCAGGCCCCTCACGCCCTGGCTCATCTGCTAATCACGCGCAACAACCCATTGTGGATGATATGCTTCCACACTCTGGCCCACTAGCCCATCATATGTCGCAGCCCACTTTGCGTGACCTGCAGGTTGCCATGCCTCATCTTTCTGCCGAGCAGCATCATCGTCTCCTTGCTGCCCTGAATGACACCACGACACCAACTCCTCAGGCTCATGCCGCTTCCCGTGATCCCTTTGCCATAGGTTTGTTACCCGTGGCTTCTCGTCATGGTCAATGGATCATTGACAGTGGCGCTACGAATCATATCACTTCCTCCGCTTCATGTTTGGTTAATACTTCTGCTTCGTACCTGCCACCTGTTTCTTTGCCTAGCGGTGACACTGCTCCAATTACTTTAACTGGCATTCTTCGTTTAAATTCCTCGATTGATTTGGAAGATGTTTTATGTGTCCCTAATTTTAAAGTGGACCTTTTATCGGTTGGTAAACTTACAGATGGATTATCGTGTTCCGTAACCTTTTTTCCTTCTTGGTGTGTTTTGCAGGACTTGGTTTCGAAGGTGATGATTGGTGTGGGTAAGCGACGTGGTGACCTTTATTACCTCGTGGCCCTTACCTATTCACTCTCCACCCGTCACCCTCTATGCAACCTCATCACCATCCCATCCTTATTATGGCATCGACGTCTTGGTCACCCTTCTCCTAGCCGTTTGCAAGTTTTAGCCTCTAATTTCCTTAATTGTTCATTTGATTCTAGTCATGTTTGTGATGTTTGTCCTTTGGCCAAACAAACTCGTCAACCTTTTGGTTTAAGTTCAATTTCTACAACTTTTCCTTTTGCTTTAATTCATTGTGATATTTGGGGTCCGAATCGTCAATCTTCCCTTACTGGTGCTCATTATTTTTTGACTATTGTGGATGATTTTTCCCGCTTTACGTGGGTCCTCCTTACTACAACCCCATCCTATGACCACTTTCGCGTCTTTGGTTGCCTAGCTTATGCCGCCACCTCTATCCATCCTACCACAAAATTTTCCCCTAGGGCCCAACGATGCGCTTTCATTGGTTACCCTCATGGCCAAAAAGCATACAAATTATATGACTTAGAAACTCATCGCACCTTCACCAGTAGAGATGTTATTTTCCATGAAGATTCCTTCCCTTTTGCTGCCACATCCCCACCTACCCAATCCCCCACACCTTCTCTGCCCATTCCCCCTTCCCCAGACCTTTCCTATCCCTCCCCTCCTGGCCCCACGTCCCTTTCATATCCTCCTACATTACCCATTTCCCTGCCTCCTGTAACCGACCCCGCATCACCCACTCCTCACATTTCGATCCCGGCCCCACCTGTCCCTTCCTCCACCGACCCTCACACCACTTCTCCTTCCCTACCCACAGCCCCTCTTAGTACCCCATCGCCCACATCACCCACGGCCCATCCACCTCCTTTACCAGCCACCCGCACCTCATCTCGCACTCACCACCCCCCGTCCTATTTAAAAGACTATGTTTGCTCGCAGGTGATTCTGCCTCCACACCCATCGTCTTCGCCGCCCAGTTCTGTCAAAGAGGCTGCTCATGATCCTAATTGGCGTCGTGCCATGAATGAGGAACTTGAGGCTTTACATGCCAACGGTACTTGGACATTGACTCCCTTACCTCCTGGCAAAGTTCCTATTGATTGTAAGTGGGTGTACAAACTAAAGCATAATTCTGATGGTTCTATTGAGCGTTACAAAGCTCGCTTGGTTGCTAAGGGTTTCACTCAAGCCGCAGGCAATGATGTCAGTGCCATTGATTCTCTAAAGTCTTTTCTTCGAGATCACTTTCGAATAAAAGATCTTGGTAATTTAAAATATTTTTTGGGTATTGAGGTTTCTCGATCTCAACGTGGGATTTATATTTCACAACGAAAGTATGCACTAGAGATCCTCAAGGATTATGGTTTTCTGGGAGCACGGCCGATTGCTTTCCCTATGGATGATACAAAATTATCTGATCAAGGAGAACTTCTCAAGGATCCTGAAAAGTATCGGCGGTTAATAGGACGGTTAATCTACCTCACCATCACTCGGCCGGATATCACATATTCTGTGCATGTTTTTAGTCGGTTTATGCATGAGCCATGTGTACCTCATATGGATGCCGCGCTTCGTGTTGTTCGTTATTTGAAGTCTACTCCAGGCCAAGGTTTATTATTCCGTTCTGACAACCAAACCAAGTTAGTTGCATTTTGTGACTCTGACTGGGCAGGTTGTCCTATCACTCGTCGTTCCACTTCTGGGTATTGTGTTTTCTTAGGCAATTCTTTGATTTCGTGGTGGACGAAGCGACAGAAAACTGTTTCACTTTCTTCAGCTGAGGCAGAATATAGGGCTATGGTCGATGCTTGTTGGTTCGCATTCCATGAGCGTGTGGTGGTTGGCACCACAATTCCTGATCATTGGGTTTGGAGATGGGTTTACTCTTGTGGCCCTTGTGGGGTTGCAGGAGTATTTTACGACCAAGTCCCGGACTCCATGAGAAGCTTAGGCATTGCTTTTTACCTCAGTGTGATCGGAGCTGGGAACTTCGTTAGCAGCTTTGTGATCACGGCCGTTGATCACATCACGGATAATGGAGGGAAGAGTTGGTTAGGTAAGGATTTGAACAACAGTAGGTTGGACAGGTTTTATTGGCTCCTGGCTTGCATGGCAGCAGCAAATCTGTGTGTTTATGTTTTCGCGGCTCGGCGTTATTCTTACAAGAATGTGCAGAAGGTAGCTGTGGCTGATTGCTTTGGAGATGAACTGGAAAATGGAGGCCCGATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000275 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005753 GO:0005754 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016462 GO:0016469 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019829 GO:0019866 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0033178 GO:0034220 GO:0034641 GO:0034654 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044769 GO:0045259 GO:0045261 GO:0045267 GO:0046034 GO:0046390 GO:0046483 GO:0046689 GO:0046933 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko00195 ko00904 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map00195 map00904 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N FliG_C FliG_M FliG_N FliH Photo_RC

Protein Analysis

1586

Amino Acids

175.4

Weight (kDa)

9.14

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 70 - 359 2.2e-55 POT family
Retrotran_gag_3 PF14244 367 - 400 2.4e-10 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 380 - 552 9.7e-07 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 739 - 812 2.8e-10 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 861 - 910 6.5e-12 GAG-pre-integrase domain
SH3_retrovirus PF25597 977 - 1038 2.2e-20 Retroviral polymerase SH3-like domain
RVT_2 PF07727 1192 - 1243 1.1e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 1240 - 1307 5.3e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3451
AasI GACNNNNNNGTC 1 cut(s) 2628
AatII GACGTC 1 cut(s) 2626
Acc36I ACCTGC 6 cut(s) 1859, 2056, 2070, 2298, 3451, 4184
AccB1I GGYRCC 3 cut(s) 812, 1508, 4367
AccB7I CCANNNNNTGG 2 cut(s) 134, 2018
AccI GTMKAC 1 cut(s) 4118
AccII CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
AclI AACGTT 1 cut(s) 1351
AcoI YGGCCR 6 cut(s) 1813, 2721, 3016, 3902, 4016, 4536
AcsI RAATTY 4 cut(s) 722, 1584, 2356, 2969
AcuI CTGAAG 1 cut(s) 4281
AcyI GRCGYC 2 cut(s) 2623, 3535
AfaI GTAC 6 cut(s) 1675, 2288, 3352, 3580, 3638, 4074
AflII CTTAAG 1 cut(s) 1150
AgsI TTSAA 6 cut(s) 172, 304, 1193, 2757, 4114, 4591
AhdI GACNNNNNGTC 1 cut(s) 216
AjiI CACGTC 2 cut(s) 2525, 3198
AloI GAACNNNNNNTCC 2 cut(s) 1341, 1373
Alw21I GWGCWC 3 cut(s) 1473, 2836, 3901
Alw26I GTCTC 6 cut(s) 9, 682, 1435, 1556, 1834, 2111
ApaI GGGCCC 1 cut(s) 2986
ApoI RAATTY 4 cut(s) 722, 1584, 2356, 2969
AseI ATTAAT 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 1302
AspA2I CCTAGG 1 cut(s) 2978
AspLEI GCGC 7 cut(s) 656, 1157, 1242, 1984, 2234, 2996, 4093
AsuC2I CCSGG 2 cut(s) 3281, 4454
AsuII TTCGAA 2 cut(s) 2495, 3772
AsuNHI GCTAGC 1 cut(s) 751
AvaII GGWCC 9 cut(s) 589, 850, 1213, 1419, 1579, 1929, 2407, 2804, 2881
AvrII CCTAGG 1 cut(s) 2978
AxyI CCTNAGG 1 cut(s) 2145
BaeGI GKGCMC 4 cut(s) 168, 1132, 1660, 2986
BalI TGGCCA 3 cut(s) 1815, 2723, 3018
BamHI GGATCC 2 cut(s) 1266, 3961
BanI GGYRCC 3 cut(s) 812, 1508, 4367
BanII GRGCYC 4 cut(s) 485, 1287, 1473, 2986
BauI CACGAG 2 cut(s) 613, 2543
BbsI GAAGAC 2 cut(s) 1713, 3480
Bbv12I GWGCWC 3 cut(s) 1473, 2836, 3901
BbvCI CCTCAGC 1 cut(s) 4302
BceAI ACGGC 7 cut(s) 1819, 1954, 2631, 3390, 3917, 4523, 4551
BcgI CGANNNNNNTGC 2 cut(s) 2084, 2118
BciVI GTATCC 1 cut(s) 1815
BclI TGATCA 5 cut(s) 645, 3940, 4381, 4530, 4543
BcnI CCSGG 2 cut(s) 3281, 4454
BcoDI GTCTC 6 cut(s) 9, 682, 1435, 1556, 1834, 2111
BfmI CTRYAG 3 cut(s) 408, 1480, 2063
BfoI RGCGCY 3 cut(s) 1158, 1243, 2235
BfrI CTTAAG 1 cut(s) 1150
BfuAI ACCTGC 6 cut(s) 1859, 2056, 2070, 2298, 3451, 4184
BfuI GTATCC 1 cut(s) 1815
BglII AGATCT 1 cut(s) 3781
BlnI CCTAGG 1 cut(s) 2978
BlpI GCTNAGC 1 cut(s) 1942
Bme18I GGWCC 9 cut(s) 589, 850, 1213, 1419, 1579, 1929, 2407, 2804, 2881
BmeRI GACNNNNNGTC 1 cut(s) 216
BmgBI CACGTC 2 cut(s) 2525, 3198
BmrI ACTGGG 4 cut(s) 222, 486, 3492, 4198
BmtI GCTAGC 1 cut(s) 755
BmuI ACTGGG 4 cut(s) 222, 486, 3492, 4198
BpiI GAAGAC 2 cut(s) 1713, 3480
BplI GAGNNNNNCTC 6 cut(s) 1460, 1492, 1831, 1863, 3939, 3971
BpmI CTGGAG 3 cut(s) 254, 1301, 4107
Bpu10I CCTNAGC 4 cut(s) 1323, 3696, 4302, 4471
Bpu1102I GCTNAGC 1 cut(s) 1942
Bpu14I TTCGAA 2 cut(s) 2495, 3772
BpuEI CTTGAG 6 cut(s) 59, 1623, 3578, 3693, 3860, 3941
BpuMI CCSGG 2 cut(s) 3281, 4454
BsaAI YACGTR 2 cut(s) 1673, 2877
BsaBI GATNNNNATC 3 cut(s) 264, 986, 1142
BsaHI GRCGYC 2 cut(s) 2623, 3535
BsaI GGTCTC 4 cut(s) 9, 1435, 1556, 1834
BsaWI WCCGGW 2 cut(s) 947, 1807
Bse1I ACTGG 9 cut(s) 217, 481, 1360, 2344, 2833, 3066, 3498, 4193, 4737
Bse21I CCTNAGG 1 cut(s) 2145
Bse3DI GCAATG 5 cut(s) 519, 1252, 1744, 3727, 4476
Bse8I GATNNNNATC 3 cut(s) 264, 986, 1142
BseJI GATNNNNATC 3 cut(s) 264, 986, 1142
BseMI GCAATG 5 cut(s) 519, 1252, 1744, 3727, 4476
BseMII CTCAG 7 cut(s) 637, 660, 926, 1956, 2159, 4293, 4504
BseNI ACTGG 9 cut(s) 217, 481, 1360, 2344, 2833, 3066, 3498, 4193, 4737
BseRI GAGGAG 7 cut(s) 911, 1414, 1704, 1728, 1836, 2133, 3255
BseSI GKGCMC 4 cut(s) 168, 1132, 1660, 2986
BseX3I CGGCCG 3 cut(s) 3902, 4016, 4536
BseYI CCCAGC 1 cut(s) 4505
BsgI GTGCAG 1 cut(s) 4715
Bsh1236I CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
Bsh1285I CGRYCG 4 cut(s) 1732, 3905, 4019, 4539
BshNI GGYRCC 3 cut(s) 812, 1508, 4367
BsiEI CGRYCG 4 cut(s) 1732, 3905, 4019, 4539
BsiHKAI GWGCWC 3 cut(s) 1473, 2836, 3901
BsiSI CCGG 5 cut(s) 948, 1808, 3281, 4019, 4454
BsmAI GTCTC 6 cut(s) 9, 682, 1435, 1556, 1834, 2111
BsmBI CGTCTC 1 cut(s) 2111
BsmI GAATGC 2 cut(s) 2343, 4343
Bso31I GGTCTC 4 cut(s) 9, 1435, 1556, 1834
Bsp119I TTCGAA 2 cut(s) 2495, 3772
Bsp120I GGGCCC 1 cut(s) 2982
Bsp1286I GDGCHC 9 cut(s) 168, 485, 1132, 1287, 1473, 1660, 2836, 2986, 3901
Bsp1407I TGTACA 1 cut(s) 3636
Bsp1720I GCTNAGC 1 cut(s) 1942
Bsp19I CCATGG 1 cut(s) 592
BspCNI CTCAG 7 cut(s) 636, 661, 927, 1955, 2158, 4294, 4503
BspFNI CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
BspHI TCATGA 2 cut(s) 1345, 3520
BspMAI CTGCAG 2 cut(s) 412, 2067
BspMI ACCTGC 6 cut(s) 1859, 2056, 2070, 2298, 3451, 4184
BspOI GCTAGC 1 cut(s) 755
BspT104I TTCGAA 2 cut(s) 2495, 3772
BspT107I GGYRCC 3 cut(s) 812, 1508, 4367
BspTI CTTAAG 1 cut(s) 1150
BspTNI GGTCTC 4 cut(s) 9, 1435, 1556, 1834
BsrDI GCAATG 5 cut(s) 519, 1252, 1744, 3727, 4476
BsrGI TGTACA 1 cut(s) 3636
BsrI ACTGG 9 cut(s) 217, 481, 1360, 2344, 2833, 3066, 3498, 4193, 4737
BssNI GRCGYC 2 cut(s) 2623, 3535
BssSI CACGAG 2 cut(s) 613, 2543
BssT1I CCWWGG 8 cut(s) 592, 683, 705, 1209, 1414, 1660, 2978, 4129
Bst2BI CACGAG 2 cut(s) 613, 2543
Bst6I CTCTTC 1 cut(s) 4562
BstACI GRCGYC 2 cut(s) 2623, 3535
BstAFI CTTAAG 1 cut(s) 1150
BstAUI TGTACA 1 cut(s) 3636
BstBAI YACGTR 2 cut(s) 1673, 2877
BstBI TTCGAA 2 cut(s) 2495, 3772
BstDSI CCRYGG 7 cut(s) 373, 592, 1825, 1835, 1936, 2193, 3372
BstEII GGTNACC 3 cut(s) 2528, 2630, 3005
BstENI CCTNNNNNAGG 1 cut(s) 1234
BstFNI CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
BstH2I RGCGCY 3 cut(s) 1158, 1243, 2235
BstHHI GCGC 7 cut(s) 656, 1157, 1242, 1984, 2234, 2996, 4093
BstMAI GTCTC 6 cut(s) 9, 682, 1435, 1556, 1834, 2111
BstMCI CGRYCG 4 cut(s) 1732, 3905, 4019, 4539
BstNSI RCATGY 2 cut(s) 3572, 4043
BstPI GGTNACC 3 cut(s) 2528, 2630, 3005
BstSFI CTRYAG 3 cut(s) 408, 1480, 2063
BstSLI GKGCMC 4 cut(s) 168, 1132, 1660, 2986
BstUI CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
BstV2I GAAGAC 2 cut(s) 1713, 3480
BstX2I RGATCY 4 cut(s) 1266, 3781, 3870, 3961
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 4 cut(s) 1266, 3781, 3870, 3961
BstZI CGGCCG 3 cut(s) 3902, 4016, 4536
Bsu36I CCTNAGG 1 cut(s) 2145
BsuI GTATCC 1 cut(s) 1815
BtgI CCRYGG 7 cut(s) 373, 592, 1825, 1835, 1936, 2193, 3372
BtgZI GCGATG 3 cut(s) 991, 3038, 3342
BtrI CACGTC 2 cut(s) 2525, 3198
BtsI GCAGTG 2 cut(s) 405, 2319
BtsIMutI CAGTG 7 cut(s) 119, 128, 405, 2233, 2319, 3736, 4498
BveI ACCTGC 6 cut(s) 1859, 2056, 2070, 2298, 3451, 4184
CciI TCATGA 2 cut(s) 1345, 3520
CfoI GCGC 7 cut(s) 656, 1157, 1242, 1984, 2234, 2996, 4093
CseI GACGC 2 cut(s) 2908, 3524
Csp6I GTAC 6 cut(s) 1674, 2287, 3351, 3579, 3637, 4073
CviQI GTAC 6 cut(s) 1674, 2287, 3351, 3579, 3637, 4073
DraI TTTAAA 4 cut(s) 2355, 2401, 3441, 3795
DrdI GACNNNNNNGTC 1 cut(s) 2628
DriI GACNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 2628
EaeI YGGCCR 6 cut(s) 1813, 2721, 3016, 3902, 4016, 4536
EagI CGGCCG 3 cut(s) 3902, 4016, 4536
Eam1104I CTCTTC 1 cut(s) 4562
Eam1105I GACNNNNNGTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 4562
EciI GGCGGA 2 cut(s) 466, 1604
Ecl136II GAGCTC 1 cut(s) 1471
EclXI CGGCCG 3 cut(s) 3902, 4016, 4536
Eco130I CCWWGG 8 cut(s) 592, 683, 705, 1209, 1414, 1660, 2978, 4129
Eco147I AGGCCT 1 cut(s) 1622
Eco24I GRGCYC 4 cut(s) 485, 1287, 1473, 2986
Eco31I GGTCTC 4 cut(s) 9, 1435, 1556, 1834
Eco32I GATATC 1 cut(s) 4024
Eco47I GGWCC 9 cut(s) 589, 850, 1213, 1419, 1579, 1929, 2407, 2804, 2881
Eco52I CGGCCG 3 cut(s) 3902, 4016, 4536
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 1 cut(s) 4281
Eco81I CCTNAGG 1 cut(s) 2145
Eco91I GGTNACC 3 cut(s) 2528, 2630, 3005
EcoICRI GAGCTC 1 cut(s) 1471
EcoNI CCTNNNNNAGG 1 cut(s) 1234
EcoO109I RGGNCCY 6 cut(s) 850, 1213, 1419, 1949, 2881, 2982
EcoO65I GGTNACC 3 cut(s) 2528, 2630, 3005
EcoRV GATATC 1 cut(s) 4024
EcoT14I CCWWGG 8 cut(s) 592, 683, 705, 1209, 1414, 1660, 2978, 4129
EcoT22I ATGCAT 3 cut(s) 66, 1492, 4062
EcoT38I GRGCYC 4 cut(s) 485, 1287, 1473, 2986
ErhI CCWWGG 8 cut(s) 592, 683, 705, 1209, 1414, 1660, 2978, 4129
Esp3I CGTCTC 1 cut(s) 2111
FalI AAGNNNNNCTT 4 cut(s) 2394, 2426, 3936, 3968
FauI CCCGC 7 cut(s) 1127, 1696, 1784, 1886, 2876, 3259, 3410
FauNDI CATATG 2 cut(s) 2037, 4080
FbaI TGATCA 5 cut(s) 645, 3940, 4381, 4530, 4543
FblI GTMKAC 1 cut(s) 4118
FriOI GRGCYC 4 cut(s) 485, 1287, 1473, 2986
GlaI GCGC 7 cut(s) 655, 1156, 1241, 1983, 2233, 2995, 4092
GsaI CCCAGC 1 cut(s) 4509
GsuI CTGGAG 3 cut(s) 254, 1301, 4107
HaeII RGCGCY 3 cut(s) 1158, 1243, 2235
HapII CCGG 5 cut(s) 948, 1808, 3281, 4019, 4454
HgaI GACGC 2 cut(s) 2908, 3524
HhaI GCGC 7 cut(s) 656, 1157, 1242, 1984, 2234, 2996, 4093
Hin1I GRCGYC 2 cut(s) 2623, 3535
Hin6I GCGC 7 cut(s) 654, 1155, 1240, 1982, 2232, 2994, 4091
HinP1I GCGC 7 cut(s) 654, 1155, 1240, 1982, 2232, 2994, 4091
HincII GTYRAC 2 cut(s) 1526, 2738
HindII GTYRAC 2 cut(s) 1526, 2738
HindIII AAGCTT 2 cut(s) 1793, 4468
HpaII CCGG 5 cut(s) 948, 1808, 3281, 4019, 4454
Hpy166II GTNNAC 9 cut(s) 1526, 2407, 2426, 2738, 3637, 4073, 4119, 4266, 4405
Hpy8I GTNNAC 9 cut(s) 1526, 2407, 2426, 2738, 3637, 4073, 4119, 4266, 4405
Hpy99I CGWCG 4 cut(s) 2526, 2625, 3540, 4215
HpyCH4IV ACGT 8 cut(s) 1351, 1654, 1672, 2524, 2623, 2876, 3197, 3832
HpySE526I ACGT 8 cut(s) 1351, 1654, 1672, 2524, 2623, 2876, 3197, 3832
Hsp92I GRCGYC 2 cut(s) 2623, 3535
HspAI GCGC 7 cut(s) 654, 1155, 1240, 1982, 2232, 2994, 4091
Ksp22I TGATCA 5 cut(s) 645, 3940, 4381, 4530, 4543
LmnI GCTCC 9 cut(s) 245, 480, 1282, 1375, 1900, 2329, 3896, 4502, 4626
MaeII ACGT 8 cut(s) 1351, 1654, 1672, 2524, 2623, 2876, 3197, 3832
MfeI CAATTG 2 cut(s) 304, 560
MflI RGATCY 4 cut(s) 1266, 3781, 3870, 3961
MhlI GDGCHC 9 cut(s) 168, 485, 1132, 1287, 1473, 1660, 2836, 2986, 3901
MlsI TGGCCA 3 cut(s) 1815, 2723, 3018
MluNI TGGCCA 3 cut(s) 1815, 2723, 3018
MlyI GAGTC 4 cut(s) 751, 3586, 4175, 4451
MmeI TCCRAC 1 cut(s) 4584
Mox20I TGGCCA 3 cut(s) 1815, 2723, 3018
Mph1103I ATGCAT 3 cut(s) 66, 1492, 4062
MroXI GAANNNNTTC 1 cut(s) 1302
MscI TGGCCA 3 cut(s) 1815, 2723, 3018
MslI CAYNNNNRTG 3 cut(s) 2703, 4083, 4353
Msp20I TGGCCA 3 cut(s) 1815, 2723, 3018
MspA1I CMGCKG 3 cut(s) 1025, 1691, 4301
MspCI CTTAAG 1 cut(s) 1150
MspI CCGG 5 cut(s) 948, 1808, 3281, 4019, 4454
MunI CAATTG 2 cut(s) 304, 560
Mva1269I GAATGC 2 cut(s) 2343, 4343
MvnI CGCG 6 cut(s) 1134, 1779, 1982, 2919, 4091, 4667
NciI CCSGG 2 cut(s) 3281, 4454
NcoI CCATGG 1 cut(s) 592
NdeI CATATG 2 cut(s) 2037, 4080
NheI GCTAGC 1 cut(s) 751
NmeAIII GCCGAG 4 cut(s) 1705, 2116, 3994, 4651
NsiI ATGCAT 3 cut(s) 66, 1492, 4062
NspI RCATGY 2 cut(s) 3572, 4043
NspV TTCGAA 2 cut(s) 2495, 3772
PacI TTAATTAA 1 cut(s) 42
PagI TCATGA 2 cut(s) 1345, 3520
PaqCI CACCTGC 1 cut(s) 3451
PceI AGGCCT 1 cut(s) 1622
PcsI WCGNNNNNNNCGW 3 cut(s) 3829, 4102, 4265
PctI GAATGC 2 cut(s) 2343, 4343
PdmI GAANNNNTTC 1 cut(s) 1302
PflFI GACNNNGTC 1 cut(s) 4448
PflMI CCANNNNNTGG 2 cut(s) 134, 2018
PfoI TCCNGGA 1 cut(s) 4452
Ple19I CGATCG 1 cut(s) 1732
PleI GAGTC 4 cut(s) 751, 3586, 4175, 4451
PpsI GAGTC 4 cut(s) 751, 3586, 4175, 4451
Ppu21I YACGTR 2 cut(s) 1673, 2877
PpuMI RGGWCCY 4 cut(s) 850, 1213, 1419, 2881
PshBI ATTAAT 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 1473
Psp1406I AACGTT 1 cut(s) 1351
Psp5II RGGWCCY 4 cut(s) 850, 1213, 1419, 2881
PspEI GGTNACC 3 cut(s) 2528, 2630, 3005
PspFI CCCAGC 1 cut(s) 4505
PspOMI GGGCCC 1 cut(s) 2982
PspPPI RGGWCCY 4 cut(s) 850, 1213, 1419, 2881
PstI CTGCAG 2 cut(s) 412, 2067
PsuI RGATCY 4 cut(s) 1266, 3781, 3870, 3961
PsyI GACNNNGTC 1 cut(s) 4448
PvuI CGATCG 1 cut(s) 1732
PvuII CAGCTG 2 cut(s) 1025, 4301
RsaI GTAC 6 cut(s) 1675, 2288, 3352, 3580, 3638, 4074
RsaNI GTAC 6 cut(s) 1674, 2287, 3351, 3579, 3637, 4073
RseI CAYNNNNRTG 3 cut(s) 2703, 4083, 4353
SacI GAGCTC 1 cut(s) 1473
SbfI CCTGCAGG 1 cut(s) 2067
SchI GAGTC 4 cut(s) 751, 3586, 4175, 4451
SdaI CCTGCAGG 1 cut(s) 2067
SduI GDGCHC 9 cut(s) 168, 485, 1132, 1287, 1473, 1660, 2836, 2986, 3901
SfcI CTRYAG 3 cut(s) 408, 1480, 2063
SfuI TTCGAA 2 cut(s) 2495, 3772
SinI GGWCC 9 cut(s) 589, 850, 1213, 1419, 1579, 1929, 2407, 2804, 2881
SmiMI CAYNNNNRTG 3 cut(s) 2703, 4083, 4353
SmlI CTYRAG 7 cut(s) 74, 1150, 1638, 3557, 3708, 3875, 3956
SmoI CTYRAG 7 cut(s) 74, 1150, 1638, 3557, 3708, 3875, 3956
Sse8387I CCTGCAGG 1 cut(s) 2067
SseBI AGGCCT 1 cut(s) 1622
SspI AATATT 1 cut(s) 3800
SstI GAGCTC 1 cut(s) 1473
StuI AGGCCT 1 cut(s) 1622
StyI CCWWGG 8 cut(s) 592, 683, 705, 1209, 1414, 1660, 2978, 4129
TaiI ACGT 8 cut(s) 1354, 1657, 1675, 2527, 2626, 2879, 3200, 3835
TaqII GACCGA 3 cut(s) 1417, 1707, 1917
TatI WGTACW 1 cut(s) 3636
TauI GCSGC 6 cut(s) 2159, 2945, 3496, 3716, 4091, 4670
TscAI CASTG 7 cut(s) 126, 135, 412, 2233, 2326, 3736, 4498
TspGWI ACGGA 5 cut(s) 889, 1122, 2440, 4133, 4568
TspRI CASTG 7 cut(s) 126, 135, 412, 2233, 2326, 3736, 4498
Tth111I GACNNNGTC 1 cut(s) 4448
Van91I CCANNNNNTGG 2 cut(s) 134, 2018
Vha464I CTTAAG 1 cut(s) 1150
VpaK11BI GGWCC 9 cut(s) 589, 850, 1213, 1419, 1579, 1929, 2407, 2804, 2881
VspI ATTAAT 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 1234
XapI RAATTY 4 cut(s) 722, 1584, 2356, 2969
XceI RCATGY 2 cut(s) 3572, 4043
XcmI CCANNNNNNNNNTGG 3 cut(s) 1012, 1796, 2610
XmaJI CCTAGG 1 cut(s) 2978
XmiI GTMKAC 1 cut(s) 4118
XmnI GAANNNNTTC 1 cut(s) 1302
ZraI GACGTC 1 cut(s) 2624
Zsp2I ATGCAT 3 cut(s) 66, 1492, 4062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.