MD12G1213700.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005906
Physical Location & Seq
Reverse (-)
29200997 .. 29202937
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213700.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGCCTACAGCTGAAGCTAAACCAGAACAACCGAAAGGAAGTGAAGAAGTTTTTCAACCCCCTCTCAACTACAAAATTTGGGTTTTGAAAGTCCCTATCCACTGTGAAGGCTGCAAGAAAAAAGTCGCAAAAATCTTGAAAAAAATCGATGGGGTTTACAAAACAGAGTTTGACGGGAACATCGGAAAGGAAAACAAAGTTACAGTGACTGGAAACGTGGAGCCGGAGATTCTGATCAAGAAACTGGCTAAGGGAGGAAAATATGCAGAGATGTGGCCTTGTCAGAAGGCTAACAACAGCAATAATTCAAATGACAAGAAGAAAAATAAGGAGAAGAATAAGGAGAAGCAGCAAGGCGAAGATACCCAAGGGTCCGAGGACGGCAACCATGGCGGAACTGGAGGCGGCGGTAGTTGTGATAATGAAAGGGAGACGGTTGAGGCTGACGTTGTTCAAGTTCAGGATAATCGTGGTAAGAAAAATAAAGGTGGTAGTGGCGCGGGTGGGAGTAAGACTCCCGAGGGGGTTAATGCTGTGAAAGTGAATGAGGGGGATGGTGCACCGGCGAAATCCGGCGGCGGTGGAAAGGGTAAAGAGGTAAAGCTTGAGGTGGTGAAGCAGGGGAAGCCTGTGAACATGTCTGGACAGCCGGCTGCGGCGGAGAAGTGTTTTGGTGGGGATGACGATGAAGATGACAGTAATTGTGAGGTTGATAAAAGTGGAGGCGGCGGCGGCCCTAGTAGCAGTGGAACTAAGAAGAACAAGAAGGGGCCAAAGGGGAAGGCCAATGCTGATGATTCAGATGAGGTTGACCAATGTGGTGATGCTGCTCCTCCACGCACTGGATCACCACCAAATCAAGGCAATGTACGACGTGGGCCTCCTATGCCTCAGGGGCCTTACATGCTTCCATCTGGTACTCAAGGCCCTAATTTTGCTCCAGCTCTTGCTCCGGCTCCGGCTTCAGCACCAGCTCCAGCCAATCACATGGCTCCACATCAGCAGATGTATGAGTACCAGTATCCCAGGCAGAACTACAGCGGGCTTCCCCTCCAAGCTATGAACTTCAATACGGCCTACCCCGCCAGGAGCTACGGTGCATCCCAGTATGCTGCACCACTACCTCACGCACACTCTTACACTTATGCGTCTCAAAGTGTGGCTGCTGAGAGTGAGCTCAGGTCCTATCATTCGGACTCGTATCCGCAGTATTACCCGTCGCCGCCGGAGTATAATTCGCCGCAGCAGTCCGATTCCTTCGTGATGTTCAGCGACGAAAATCCGAACGGGTGCAGCATTATGTGA

Protein Analysis

435

Amino Acids

46.03

Weight (kDa)

6.68

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 29 - 85 2.6e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 842
AccII CGCG 1 cut(s) 500
AclWI GGATC 1 cut(s) 853
AcsI RAATTY 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 33, 948
AfaI GTAC 3 cut(s) 870, 919, 1016
AfiI CCNNNNNNNGG 2 cut(s) 842, 860
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 6 cut(s) 56, 88, 139, 309, 455, 1069
AjiI CACGTC 1 cut(s) 875
AjnI CCWGG 2 cut(s) 1025, 1085
AluBI AGCT 8 cut(s) 11, 17, 604, 944, 974, 1058, 1092, 1177
AluI AGCT 8 cut(s) 11, 17, 604, 944, 974, 1058, 1092, 1177
Alw21I GWGCWC 2 cut(s) 562, 1179
Alw26I GTCTC 2 cut(s) 425, 1155
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 1 cut(s) 853
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 8 cut(s) 275, 733, 770, 783, 878, 896, 925, 1074
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 8 cut(s) 111, 349, 653, 827, 1112, 1163, 1243, 1293
ApoI RAATTY 1 cut(s) 75
Asp700I GAANNNNTTC 1 cut(s) 51
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 7 cut(s) 372, 734, 770, 878, 896, 926, 1182
AsuHPI GGTGA 3 cut(s) 625, 833, 840
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 372, 1182
AxyI CCTNAGG 1 cut(s) 891
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 1179
Bbv12I GWGCWC 2 cut(s) 562, 1179
BbvI GCAGC 7 cut(s) 98, 361, 640, 814, 1099, 1150, 1255
BccI CCATC 3 cut(s) 143, 548, 919
BceAI ACGGC 2 cut(s) 397, 1089
BciT130I CCWGG 2 cut(s) 1027, 1087
BciVI GTATCC 2 cut(s) 1032, 1212
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 425, 1155
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 2 cut(s) 6, 1036
BfuI GTATCC 2 cut(s) 1032, 1212
BglI GCCNNNNNGGC 1 cut(s) 895
Bme1390I CCNGG 2 cut(s) 1027, 1087
Bme18I GGWCC 2 cut(s) 372, 1182
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 875
BmgT120I GGNCC 7 cut(s) 372, 734, 770, 878, 896, 926, 1182
BmiI GGNNCC 6 cut(s) 222, 373, 771, 897, 957, 993
BmrFI CCNGG 2 cut(s) 1027, 1087
BmrI ACTGGG 1 cut(s) 1099
BmsI GCATC 2 cut(s) 814, 1109
BmuI ACTGGG 1 cut(s) 1099
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 3 cut(s) 420, 924, 960
Bpu10I CCTNAGC 2 cut(s) 249, 1178
BpuEI CTTGAG 2 cut(s) 626, 906
Bsa29I ATCGAT 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 233
BsaJI CCNNGG 5 cut(s) 367, 375, 388, 519, 1025
BsaXI ACNNNNNCTCC 2 cut(s) 1081, 1111
Bsc4I CCNNNNNNNGG 2 cut(s) 842, 860
Bse118I RCCGGY 2 cut(s) 562, 649
Bse1I ACTGG 6 cut(s) 214, 249, 403, 847, 1018, 1105
Bse21I CCTNAGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 2 cut(s) 1027, 1087
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 5 cut(s) 367, 375, 388, 519, 1025
BseGI GGATG 3 cut(s) 559, 685, 1100
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 842, 860
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 3 cut(s) 905, 1158, 1192
BseNI ACTGG 6 cut(s) 214, 249, 403, 847, 1018, 1105
BseRI GAGGAG 1 cut(s) 822
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 7 cut(s) 98, 361, 640, 814, 1099, 1150, 1255
BsgI GTGCAG 1 cut(s) 1098
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 8 cut(s) 277, 735, 772, 785, 880, 898, 927, 1076
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 562, 1179
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 7 cut(s) 224, 563, 573, 650, 953, 959, 1226
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 842, 860
BsmAI GTCTC 2 cut(s) 425, 1155
BsmBI CGTCTC 2 cut(s) 425, 1155
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 8 cut(s) 277, 735, 772, 785, 880, 898, 927, 1076
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 562, 1179
Bsp143I GATC 2 cut(s) 234, 845
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 8 cut(s) 277, 735, 772, 785, 880, 898, 927, 1076
BspCNI CTCAG 3 cut(s) 904, 1159, 1191
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 6 cut(s) 222, 373, 771, 897, 957, 993
BspPI GGATC 1 cut(s) 853
BsrDI GCAATG 1 cut(s) 871
BsrFI RCCGGY 2 cut(s) 562, 649
BsrI ACTGG 6 cut(s) 214, 249, 403, 847, 1018, 1105
BssAI RCCGGY 2 cut(s) 562, 649
BssECI CCNNGG 5 cut(s) 367, 375, 388, 519, 1025
BssMI GATC 2 cut(s) 234, 845
BssT1I CCWWGG 2 cut(s) 367, 388
Bst2UI CCWGG 2 cut(s) 1027, 1087
Bst4CI ACNGT 5 cut(s) 104, 205, 436, 698, 1097
BstC8I GCNNGC 2 cut(s) 651, 1043
BstDEI CTNAG 5 cut(s) 249, 753, 891, 1167, 1178
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 3 cut(s) 559, 685, 1100
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 2 cut(s) 237, 848
BstMAI GTCTC 2 cut(s) 425, 1155
BstMBI GATC 2 cut(s) 234, 845
BstMWI GCNNNNNNNGC 8 cut(s) 390, 625, 732, 741, 886, 895, 904, 1082
BstNI CCWGG 2 cut(s) 1027, 1087
BstNSI RCATGY 2 cut(s) 640, 907
BstSCI CCNGG 2 cut(s) 1025, 1085
BstSFI CTRYAG 2 cut(s) 6, 1036
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 7 cut(s) 98, 361, 640, 814, 1099, 1150, 1255
BstXI CCANNNNNNTGG 1 cut(s) 988
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 891
BsuI GTATCC 2 cut(s) 1032, 1212
BsuRI GGCC 8 cut(s) 277, 735, 772, 785, 880, 898, 927, 1076
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 1 cut(s) 388
BtrI CACGTC 1 cut(s) 875
BtsCI GGATG 3 cut(s) 559, 685, 1100
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 4 cut(s) 100, 210, 751, 840
Cac8I GCNNGC 2 cut(s) 651, 1043
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 2 cut(s) 562, 649
Cfr13I GGNCC 7 cut(s) 372, 734, 770, 878, 896, 926, 1182
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 1137
Csp6I GTAC 3 cut(s) 869, 918, 1015
CviAII CATG 4 cut(s) 389, 637, 904, 988
CviQI GTAC 3 cut(s) 869, 918, 1015
DdeI CTNAG 5 cut(s) 249, 753, 891, 1167, 1178
DpnI GATC 2 cut(s) 236, 847
DpnII GATC 2 cut(s) 234, 845
EciI GGCGGA 2 cut(s) 408, 674
Ecl136II GAGCTC 1 cut(s) 1177
Eco130I CCWWGG 2 cut(s) 367, 388
Eco24I GRGCYC 1 cut(s) 1179
Eco47I GGWCC 2 cut(s) 372, 1182
Eco53kI GAGCTC 1 cut(s) 1177
Eco57I CTGAAG 2 cut(s) 33, 948
Eco81I CCTNAGG 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 1177
EcoO109I RGGNCCY 3 cut(s) 896, 926, 1182
EcoRII CCWGG 2 cut(s) 1025, 1085
EcoT14I CCWWGG 2 cut(s) 367, 388
EcoT38I GRGCYC 1 cut(s) 1179
ErhI CCWWGG 2 cut(s) 367, 388
Esp3I CGTCTC 2 cut(s) 425, 1155
FaeI CATG 4 cut(s) 392, 640, 907, 991
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 388, 636, 903, 987
FauI CCCGC 3 cut(s) 493, 1034, 1090
FbaI TGATCA 1 cut(s) 234
FokI GGATG 3 cut(s) 566, 692, 1087
FriOI GRGCYC 1 cut(s) 1179
FspBI CTAG 1 cut(s) 738
GlaI GCGC 1 cut(s) 499
GsuI CTGGAG 3 cut(s) 420, 924, 960
HaeIII GGCC 8 cut(s) 277, 735, 772, 785, 880, 898, 927, 1076
HapII CCGG 7 cut(s) 224, 563, 573, 650, 953, 959, 1226
HgaI GACGC 1 cut(s) 1137
HhaI GCGC 1 cut(s) 500
Hin1II CATG 4 cut(s) 392, 640, 907, 991
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 5 cut(s) 229, 514, 797, 1196, 1253
HpaII CCGG 7 cut(s) 224, 563, 573, 650, 953, 959, 1226
HphI GGTGA 3 cut(s) 625, 833, 840
Hpy166II GTNNAC 4 cut(s) 157, 560, 634, 811
Hpy188I TCNGA 8 cut(s) 185, 234, 285, 376, 802, 1195, 1252, 1284
Hpy188III TCNNGA 6 cut(s) 136, 238, 461, 518, 642, 1261
Hpy8I GTNNAC 4 cut(s) 157, 560, 634, 811
Hpy99I CGWCG 3 cut(s) 876, 1222, 1277
HpyAV CCTTC 5 cut(s) 101, 280, 760, 775, 1267
HpyCH4III ACNGT 5 cut(s) 104, 205, 436, 698, 1097
HpyCH4IV ACGT 3 cut(s) 216, 447, 874
HpyCH4V TGCA 6 cut(s) 114, 266, 560, 1100, 1115, 1293
HpyF10VI GCNNNNNNNGC 8 cut(s) 390, 625, 732, 741, 886, 895, 904, 1082
HpyF3I CTNAG 5 cut(s) 249, 753, 891, 1167, 1178
HpySE526I ACGT 3 cut(s) 216, 447, 874
Hsp92II CATG 4 cut(s) 392, 640, 907, 991
HspAI GCGC 1 cut(s) 498
KroI GCCGGC 1 cut(s) 649
KroNI GCCGGC 1 cut(s) 651
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 2 cut(s) 234, 845
LmnI GCTCC 8 cut(s) 220, 835, 943, 955, 961, 979, 997, 1089
Lsp1109I GCAGC 7 cut(s) 98, 361, 640, 814, 1099, 1150, 1255
LweI GCATC 2 cut(s) 814, 1109
MaeI CTAG 1 cut(s) 738
MaeII ACGT 3 cut(s) 216, 447, 874
MaeIII GTNAC 2 cut(s) 199, 205
MalI GATC 2 cut(s) 236, 847
MboI GATC 2 cut(s) 234, 845
MboII GAAGA 6 cut(s) 56, 331, 346, 371, 701, 769
MhlI GDGCHC 2 cut(s) 562, 1179
MluCI AATT 5 cut(s) 75, 304, 700, 931, 1234
MlyI GAGTC 2 cut(s) 508, 1190
MroNI GCCGGC 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 1 cut(s) 528
MspA1I CMGCKG 2 cut(s) 11, 1041
MspI CCGG 7 cut(s) 224, 563, 573, 650, 953, 959, 1226
MspR9I CCNGG 2 cut(s) 1027, 1087
MvaI CCWGG 2 cut(s) 1027, 1087
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 8 cut(s) 390, 625, 732, 741, 886, 895, 904, 1082
NaeI GCCGGC 1 cut(s) 651
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 234, 845
NgoMIV GCCGGC 1 cut(s) 649
NlaIII CATG 4 cut(s) 392, 640, 907, 991
NlaIV GGNNCC 6 cut(s) 222, 373, 771, 897, 957, 993
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 2 cut(s) 640, 907
PciI ACATGT 1 cut(s) 636
PdiI GCCGGC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 3 cut(s) 229, 797, 1253
PflMI CCANNNNNTGG 1 cut(s) 842
PleI GAGTC 2 cut(s) 508, 1190
PpsI GAGTC 2 cut(s) 508, 1190
PpuMI RGGWCCY 1 cut(s) 1182
PscI ACATGT 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 1179
Psp5II RGGWCCY 1 cut(s) 1182
Psp6I CCWGG 2 cut(s) 1025, 1085
PspGI CCWGG 2 cut(s) 1025, 1085
PspN4I GGNNCC 6 cut(s) 222, 373, 771, 897, 957, 993
PspPI GGNCC 7 cut(s) 372, 734, 770, 878, 896, 926, 1182
PspPPI RGGWCCY 1 cut(s) 1182
PstNI CAGNNNCTG 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 3 cut(s) 870, 919, 1016
RsaNI GTAC 3 cut(s) 869, 918, 1015
SacI GAGCTC 1 cut(s) 1179
SaqAI TTAA 1 cut(s) 528
Sau3AI GATC 2 cut(s) 234, 845
Sau96I GGNCC 7 cut(s) 372, 734, 770, 878, 896, 926, 1182
SchI GAGTC 2 cut(s) 508, 1190
ScrFI CCNGG 2 cut(s) 1027, 1087
SduI GDGCHC 2 cut(s) 562, 1179
SfaNI GCATC 2 cut(s) 814, 1109
SfcI CTRYAG 2 cut(s) 6, 1036
SgrAI CRCCGGYG 1 cut(s) 562
SinI GGWCC 2 cut(s) 372, 1182
SmlI CTYRAG 2 cut(s) 605, 921
SmoI CTYRAG 2 cut(s) 605, 921
Sse9I AATT 5 cut(s) 75, 304, 700, 931, 1234
SspMI CTAG 1 cut(s) 738
SstI GAGCTC 1 cut(s) 1179
StyD4I CCNGG 2 cut(s) 1025, 1085
StyI CCWWGG 2 cut(s) 367, 388
TaaI ACNGT 5 cut(s) 104, 205, 436, 698, 1097
TaiI ACGT 3 cut(s) 219, 450, 877
TaqI TCGA 1 cut(s) 147
TasI AATT 5 cut(s) 75, 304, 700, 931, 1234
TauI GCSGC 8 cut(s) 408, 579, 659, 729, 732, 735, 1225, 1243
TfiI GAWTC 3 cut(s) 229, 797, 1253
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 4 cut(s) 107, 210, 751, 847
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 8 cut(s) 111, 349, 653, 827, 1112, 1163, 1243, 1293
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 438, 702, 1076
TspRI CASTG 4 cut(s) 107, 210, 751, 847
Van91I CCANNNNNTGG 1 cut(s) 842
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 2 cut(s) 372, 1182
XapI RAATTY 1 cut(s) 75
XceI RCATGY 2 cut(s) 640, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.