RchiOBHm_Chr3g0455651

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005906
Physical Location & Seq
Forward (+)
5317535 .. 5319976
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42257

Sequence Viewer

Length: 1302 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCAAGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACATTACAAGGGTCGACTATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTTTGATCAAGACACTGACCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGACAATTCCAAAGACAAGAAACAGAGTAAACCCAAAGGCAATGAGAAACAGAGTGAAATCTCCGAGGAAGCAAAAGCAAACCACGGCGGCGGCGGCGGAGATAAGAAAAAGGGTAAAGAGCCGGTGAAGGATGAAGTTGTTGAAGTGCAAGAGTCTGGTGGGGGTAAGAAGAACTTTGAAGTTGGTAGTTCTAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTTAAACCACATGAGGGTGGCGGCGGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACAGCCGGCGGCGGCTGAAAAGCAAAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGAAAAGTGGAGGTGGTGCTGGCAATGGTGGTGGGGGTGGGGGTAAAAAGAAAAAGGGCAAGGGGCAGAACGGGAATCACAATGTCAATGCTGCTGAAGGTGAACTGGTTGTCAACGCACCAAGCACCGGTTCACCACCGGTTCAGCCCGGGCCTCAGCCTCAGCCTCAGCCTCATGGGCCGCCTAATGTTCAGAGCCAAGTGCCCAATGTGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGGCAGCAGCAGCAGCAGTATGAGCATCCTTACCCAAGGCAGCAGCACTATGAGCACCCTTACCCAATGCAGCAGCAACAGCACTTTACTCCTGTGCATGCGATGAACTACAATGCAGCACACCCCAGCATTAGCTACGGTGCGGCTGGCTATGCTCCACAACCTGTTTATTCTCAGTCGCACACTTATGCAAATTGGAGTTTGGGGAATGAGGTTGATCCACCAGCCTATGATTTGGACAACCTTCCGCCTGCGCCGCCGTTCCATCCTCCACAGGCCTATCCACCACATCCTTACAACTCATCTGATTCCGATACCTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCGGTATCATGTGA

Protein Analysis

433

Amino Acids

46.43

Weight (kDa)

6.63

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 4.3e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 901
Acc36I ACCTGC 1 cut(s) 901
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 1 cut(s) 1148
AcuI CTGAAG 3 cut(s) 66, 765, 1249
AfaI GTAC 1 cut(s) 929
AfiI CCNNNNNNNGG 2 cut(s) 527, 776
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 2 cut(s) 776, 787
AgsI TTSAA 5 cut(s) 9, 91, 142, 431, 467
AjuI GAANNNNNNNTTGG 2 cut(s) 763, 795
AleI CACNNNNGTG 1 cut(s) 108
AluBI AGCT 3 cut(s) 579, 641, 1071
AluI AGCT 3 cut(s) 579, 641, 1071
Alw21I GWGCWC 2 cut(s) 924, 993
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 1148
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 6 cut(s) 275, 800, 827, 866, 892, 1212
AsiGI ACCGGT 2 cut(s) 776, 787
AspLEI GCGC 1 cut(s) 1192
AspS9I GGNCC 3 cut(s) 800, 827, 893
AsuC2I CCSGG 2 cut(s) 798, 799
AsuHPI GGTGA 4 cut(s) 121, 424, 761, 774
AvaI CYCGRG 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 924, 993
BbvCI CCTCAGC 4 cut(s) 804, 810, 816, 903
BccI CCATC 2 cut(s) 146, 1209
BceAI ACGGC 2 cut(s) 388, 1180
BcgI CGANNNNNNTGC 2 cut(s) 1238, 1272
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 798, 799
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 826
Bme1390I CCNGG 2 cut(s) 798, 799
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 3 cut(s) 800, 827, 893
BmrFI CCNGG 2 cut(s) 798, 799
BmsI GCATC 1 cut(s) 970
Bpu10I CCTNAGC 4 cut(s) 804, 810, 816, 903
BpuMI CCSGG 2 cut(s) 798, 799
BsaBI GATNNNNATC 1 cut(s) 1275
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 5 cut(s) 351, 370, 797, 935, 971
BsaWI WCCGGW 2 cut(s) 776, 787
Bsc4I CCNNNNNNNGG 2 cut(s) 527, 776
Bse118I RCCGGY 4 cut(s) 409, 598, 776, 787
Bse1I ACTGG 4 cut(s) 577, 589, 759, 875
Bse3DI GCAATG 2 cut(s) 334, 679
Bse8I GATNNNNATC 1 cut(s) 1275
BseDI CCNNGG 5 cut(s) 351, 370, 797, 935, 971
BseGI GGATG 4 cut(s) 424, 961, 1201, 1225
BseJI GATNNNNATC 1 cut(s) 1275
BseLI CCNNNNNNNGG 2 cut(s) 527, 776
BseMI GCAATG 2 cut(s) 334, 679
BseMII CTCAG 5 cut(s) 818, 824, 830, 917, 1124
BseNI ACTGG 4 cut(s) 577, 589, 759, 875
BseSI GKGCMC 1 cut(s) 855
BseYI CCCAGC 2 cut(s) 584, 1061
BsgI GTGCAG 1 cut(s) 881
BshFI GGCC 6 cut(s) 277, 802, 829, 868, 894, 1214
BshTI ACCGGT 2 cut(s) 776, 787
BsiHKAI GWGCWC 2 cut(s) 924, 993
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 5 cut(s) 410, 599, 777, 788, 798
BslI CCNNNNNNNGG 2 cut(s) 527, 776
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 1259
BsnI GGCC 6 cut(s) 277, 802, 829, 868, 894, 1214
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 797
Bsp1286I GDGCHC 3 cut(s) 855, 924, 993
Bsp143I GATC 2 cut(s) 228, 1153
BspANI GGCC 6 cut(s) 277, 802, 829, 868, 894, 1214
BspCNI CTCAG 5 cut(s) 817, 823, 829, 916, 1123
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 1 cut(s) 1148
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 334, 679
BsrFI RCCGGY 4 cut(s) 409, 598, 776, 787
BsrI ACTGG 4 cut(s) 577, 589, 759, 875
BssAI RCCGGY 4 cut(s) 409, 598, 776, 787
BssECI CCNNGG 5 cut(s) 351, 370, 797, 935, 971
BssMI GATC 2 cut(s) 228, 1153
BssT1I CCWWGG 2 cut(s) 935, 971
Bst4CI ACNGT 3 cut(s) 107, 199, 1076
BstC8I GCNNGC 8 cut(s) 115, 581, 600, 670, 896, 1035, 1084, 1188
BstDEI CTNAG 5 cut(s) 804, 810, 816, 903, 1110
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 4 cut(s) 424, 961, 1201, 1225
BstHHI GCGC 1 cut(s) 1192
BstKTI GATC 2 cut(s) 231, 1156
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 228, 1153
BstNSI RCATGY 1 cut(s) 1037
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 796, 797
BstSLI GKGCMC 1 cut(s) 855
BsuRI GGCC 6 cut(s) 277, 802, 829, 868, 894, 1214
BtgI CCRYGG 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 1052
BtsCI GGATG 4 cut(s) 424, 961, 1201, 1225
BtsIMutI CAGTG 3 cut(s) 103, 236, 1273
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 8 cut(s) 115, 581, 600, 670, 896, 1035, 1084, 1188
CfoI GCGC 1 cut(s) 1192
Cfr10I RCCGGY 4 cut(s) 409, 598, 776, 787
Cfr13I GGNCC 3 cut(s) 800, 827, 893
Cfr9I CCCGGG 1 cut(s) 797
Csp6I GTAC 1 cut(s) 928
CspAI ACCGGT 2 cut(s) 776, 787
CspCI CAANNNNNGTGG 2 cut(s) 661, 696
CviAII CATG 5 cut(s) 524, 824, 890, 1034, 1297
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 5 cut(s) 804, 810, 816, 903, 1110
DpnI GATC 2 cut(s) 230, 1155
DpnII GATC 2 cut(s) 228, 1153
EciI GGCGGA 2 cut(s) 399, 1173
Eco130I CCWWGG 2 cut(s) 935, 971
Eco147I AGGCCT 2 cut(s) 868, 1214
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 66, 765, 1249
Eco88I CYCGRG 1 cut(s) 797
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 935, 971
EcoT22I ATGCAT 1 cut(s) 1261
ErhI CCWWGG 2 cut(s) 935, 971
FaeI CATG 5 cut(s) 527, 827, 893, 1037, 1300
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FatI CATG 5 cut(s) 523, 823, 889, 1033, 1296
FauI CCCGC 1 cut(s) 903
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 1 cut(s) 174
FokI GGATG 4 cut(s) 431, 948, 1188, 1212
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 1191
GsaI CCCAGC 2 cut(s) 588, 1065
HaeIII GGCC 6 cut(s) 277, 802, 829, 868, 894, 1214
HapII CCGG 5 cut(s) 410, 599, 777, 788, 798
HhaI GCGC 1 cut(s) 1192
Hin1II CATG 5 cut(s) 527, 827, 893, 1037, 1300
Hin6I GCGC 1 cut(s) 1190
HinP1I GCGC 1 cut(s) 1190
HincII GTYRAC 2 cut(s) 175, 763
HindII GTYRAC 2 cut(s) 175, 763
HinfI GANTC 3 cut(s) 440, 724, 1244
HpaII CCGG 5 cut(s) 410, 599, 777, 788, 798
HphI GGTGA 4 cut(s) 121, 424, 761, 774
Hpy166II GTNNAC 6 cut(s) 160, 175, 317, 752, 763, 782
Hpy188I TCNGA 5 cut(s) 46, 352, 843, 1243, 1249
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 6 cut(s) 160, 175, 317, 752, 763, 782
HpyAV CCTTC 7 cut(s) 85, 118, 409, 497, 542, 740, 1190
HpyCH4III ACNGT 3 cut(s) 107, 199, 1076
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 9 cut(s) 117, 184, 436, 862, 1006, 1033, 1052, 1127, 1259
HpyF3I CTNAG 5 cut(s) 804, 810, 816, 903, 1110
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 5 cut(s) 527, 827, 893, 1037, 1300
HspAI GCGC 1 cut(s) 1190
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 228, 1153
LmnI GCTCC 1 cut(s) 1096
LweI GCATC 1 cut(s) 970
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 127, 193, 591
MalI GATC 2 cut(s) 230, 1155
MboI GATC 2 cut(s) 228, 1153
MboII GAAGA 4 cut(s) 59, 154, 469, 1255
MhlI GDGCHC 3 cut(s) 855, 924, 993
MluCI AATT 4 cut(s) 147, 292, 554, 1129
MlyI GAGTC 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 1261
MroNI GCCGGC 1 cut(s) 598
MseI TTAA 2 cut(s) 516, 557
MslI CAYNNNNRTG 3 cut(s) 108, 528, 1122
MspI CCGG 5 cut(s) 410, 599, 777, 788, 798
MspR9I CCNGG 2 cut(s) 798, 799
Mva1269I GAATGC 1 cut(s) 1259
NaeI GCCGGC 1 cut(s) 600
NciI CCSGG 2 cut(s) 798, 799
NdeII GATC 2 cut(s) 228, 1153
NgoMIV GCCGGC 1 cut(s) 598
NlaIII CATG 5 cut(s) 527, 827, 893, 1037, 1300
NmuCI GTSAC 3 cut(s) 127, 193, 591
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 1 cut(s) 1037
OliI CACNNNNGTG 1 cut(s) 108
PaeI GCATGC 1 cut(s) 1037
PaqCI CACCTGC 1 cut(s) 901
PceI AGGCCT 2 cut(s) 868, 1214
PctI GAATGC 1 cut(s) 1259
PdiI GCCGGC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 724, 1244
PinAI ACCGGT 2 cut(s) 776, 787
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 584, 1061
PspPI GGNCC 3 cut(s) 800, 827, 893
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 3 cut(s) 108, 528, 1122
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 516, 557
Sau3AI GATC 2 cut(s) 228, 1153
Sau96I GGNCC 3 cut(s) 800, 827, 893
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 2 cut(s) 798, 799
SduI GDGCHC 3 cut(s) 855, 924, 993
SfaNI GCATC 1 cut(s) 970
SmaI CCCGGG 1 cut(s) 799
SmiMI CAYNNNNRTG 3 cut(s) 108, 528, 1122
SphI GCATGC 1 cut(s) 1037
SrfI GCCCGGGC 1 cut(s) 799
Sse9I AATT 4 cut(s) 147, 292, 554, 1129
SseBI AGGCCT 2 cut(s) 868, 1214
SspMI CTAG 1 cut(s) 480
StuI AGGCCT 2 cut(s) 868, 1214
StyD4I CCNGG 2 cut(s) 796, 797
StyI CCWWGG 2 cut(s) 935, 971
TaaI ACNGT 3 cut(s) 107, 199, 1076
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 174
TasI AATT 4 cut(s) 147, 292, 554, 1129
TauI GCSGC 9 cut(s) 378, 381, 384, 537, 605, 608, 832, 1082, 1195
TfiI GAWTC 2 cut(s) 724, 1244
Tru1I TTAA 2 cut(s) 516, 557
Tru9I TTAA 2 cut(s) 516, 557
TscAI CASTG 3 cut(s) 110, 243, 1273
TseFI GTSAC 3 cut(s) 127, 193, 591
Tsp45I GTSAC 3 cut(s) 127, 193, 591
TspDTI ATGAA 3 cut(s) 435, 1055, 1287
TspMI CCCGGG 1 cut(s) 797
TspRI CASTG 3 cut(s) 110, 243, 1273
XceI RCATGY 1 cut(s) 1037
XmaI CCCGGG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 480
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.