RLG00000025343

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005906
Physical Location & Seq
Reverse (-)
45180880 .. 45182578
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025343

Sequence Viewer

Length: 1305 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCATGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACATTACACAGGTCGACTATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTTTGATCAAGACACTGACCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGCCAATTCCAAAGACAAGAAACAGAGTAAACCCAAAGGCAATGAGAAACAGAGTGAAATCCCCAAGGAAGCAAAAGCAAACCACGGTGGCGGCGGCGGCGGAGATAAGAAAAAGGGTAAAGAGCCGGTGAAGGATGAAGTTGTTGAAGTGCAAGAGTCTGGTGGGGGTAAGAAGAACTTTGAAGTTGGTAGTTCTAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTCAAACCACATGAGGGTGGCGGCGGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACCGCCGGCGGCGGCTGAAAAGCAGAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGAAAAGTGGAGGTGGTGCTGGCAATGGCAGTGGTGGTGGGGGTAAAAAGAAAAAGGGTAAGGGGCAGAACGGGAATCACAATGTCAATGCTGCTGAAGGTGAATTGGTTGTCGATGCACCAAGCACTGGTTCACCACCGGTTCAGCCCGGGCCTCAGCCTCAGCCTCAGCCTCATGGGCCGCCTAATGTTCAGAGCCAAGTGCCCAATGTGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGGCAGCAGCAGCAGTATGAGCATCCTTACCCAAGGCAGCAGCACTGTGAGCACCCTTACCCAAGGCAGCAGCAGCAGTACTTTACTCCTGTGCATGCGATGAACTACAATGCGGCACACCCCAGCATTAGCTACGGTGCGGCTCGCTATGCTCCACAACCTGTTTATTCTCAGTCGCACACTTATGCACATAGGGGTTTGGGGAATGATGTTGATCCACCAGCCTATGATTTGGACAACCTTCCACCTGCGGTGCCATCCCATCCTCCACAGGCCTATCCACCACATCCTTACTATTCCTCTGATTCCGATAACTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCAGTATCATGTGA

Protein Analysis

435

Amino Acids

46.47

Weight (kDa)

7.71

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 3.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 907, 1198
Acc36I ACCTGC 2 cut(s) 907, 1198
AccB1I GGYRCC 1 cut(s) 1195
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 1 cut(s) 1151
AcuI CTGAAG 3 cut(s) 66, 768, 1252
AfaI GTAC 2 cut(s) 935, 1022
AfiI CCNNNNNNNGG 2 cut(s) 530, 779
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 790
AgsI TTSAA 5 cut(s) 9, 91, 142, 434, 470
AjuI GAANNNNNNNTTGG 2 cut(s) 766, 798
AleI CACNNNNGTG 1 cut(s) 108
AluBI AGCT 3 cut(s) 582, 644, 1074
AluI AGCT 3 cut(s) 582, 644, 1074
Alw21I GWGCWC 2 cut(s) 930, 996
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 1151
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 6 cut(s) 275, 803, 830, 869, 895, 1215
AsiGI ACCGGT 1 cut(s) 790
AspS9I GGNCC 3 cut(s) 803, 830, 896
AsuC2I CCSGG 2 cut(s) 801, 802
AsuHPI GGTGA 5 cut(s) 121, 427, 588, 764, 777
AvaI CYCGRG 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 858
BanI GGYRCC 1 cut(s) 1195
Bbv12I GWGCWC 2 cut(s) 930, 996
BbvCI CCTCAGC 4 cut(s) 807, 813, 819, 909
BccI CCATC 3 cut(s) 146, 1207, 1212
BcgI CGANNNNNNTGC 2 cut(s) 1241, 1275
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 801, 802
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 483
BfuAI ACCTGC 2 cut(s) 907, 1198
BglI GCCNNNNNGGC 1 cut(s) 829
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 2 cut(s) 801, 802
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 3 cut(s) 803, 830, 896
BmiI GGNNCC 1 cut(s) 1197
BmrFI CCNGG 2 cut(s) 801, 802
BmsI GCATC 2 cut(s) 757, 973
Bpu10I CCTNAGC 4 cut(s) 807, 813, 819, 909
BpuMI CCSGG 2 cut(s) 801, 802
BsaBI GATNNNNATC 2 cut(s) 1155, 1278
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 6 cut(s) 351, 370, 800, 941, 974, 1004
BsaWI WCCGGW 1 cut(s) 790
Bsc4I CCNNNNNNNGG 2 cut(s) 530, 779
Bse118I RCCGGY 3 cut(s) 412, 601, 790
Bse1I ACTGG 4 cut(s) 580, 592, 784, 878
Bse3DI GCAATG 2 cut(s) 334, 682
Bse8I GATNNNNATC 2 cut(s) 1155, 1278
BseDI CCNNGG 6 cut(s) 351, 370, 800, 941, 974, 1004
BseGI GGATG 5 cut(s) 427, 964, 1199, 1204, 1228
BseJI GATNNNNATC 2 cut(s) 1155, 1278
BseLI CCNNNNNNNGG 2 cut(s) 530, 779
BseMI GCAATG 2 cut(s) 334, 682
BseMII CTCAG 5 cut(s) 821, 827, 833, 923, 1127
BseNI ACTGG 4 cut(s) 580, 592, 784, 878
BseSI GKGCMC 1 cut(s) 858
BseYI CCCAGC 2 cut(s) 587, 1064
BsgI GTGCAG 1 cut(s) 884
BshFI GGCC 6 cut(s) 277, 805, 832, 871, 897, 1217
BshNI GGYRCC 1 cut(s) 1195
BshTI ACCGGT 1 cut(s) 790
BsiHKAI GWGCWC 2 cut(s) 930, 996
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 4 cut(s) 413, 602, 791, 801
BslI CCNNNNNNNGG 2 cut(s) 530, 779
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 1262
BsnI GGCC 6 cut(s) 277, 805, 832, 871, 897, 1217
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 3 cut(s) 858, 930, 996
Bsp143I GATC 2 cut(s) 228, 1156
BspANI GGCC 6 cut(s) 277, 805, 832, 871, 897, 1217
BspCNI CTCAG 5 cut(s) 820, 826, 832, 922, 1126
BspLI GGNNCC 1 cut(s) 1197
BspMI ACCTGC 2 cut(s) 907, 1198
BspPI GGATC 1 cut(s) 1151
BspT107I GGYRCC 1 cut(s) 1195
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 334, 682
BsrFI RCCGGY 3 cut(s) 412, 601, 790
BsrI ACTGG 4 cut(s) 580, 592, 784, 878
BssAI RCCGGY 3 cut(s) 412, 601, 790
BssECI CCNNGG 6 cut(s) 351, 370, 800, 941, 974, 1004
BssMI GATC 2 cut(s) 228, 1156
BssT1I CCWWGG 4 cut(s) 351, 941, 974, 1004
Bst4CI ACNGT 5 cut(s) 107, 199, 374, 989, 1079
BstC8I GCNNGC 7 cut(s) 115, 584, 603, 673, 899, 1038, 1087
BstDEI CTNAG 5 cut(s) 807, 813, 819, 909, 1113
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 5 cut(s) 427, 964, 1199, 1204, 1228
BstKTI GATC 2 cut(s) 231, 1159
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 228, 1156
BstNSI RCATGY 1 cut(s) 1040
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 799, 800
BstSLI GKGCMC 1 cut(s) 858
BsuRI GGCC 6 cut(s) 277, 805, 832, 871, 897, 1217
BtgI CCRYGG 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 1055
BtsCI GGATG 5 cut(s) 427, 964, 1199, 1204, 1228
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 6 cut(s) 103, 236, 688, 777, 985, 1276
BveI ACCTGC 2 cut(s) 907, 1198
Cac8I GCNNGC 7 cut(s) 115, 584, 603, 673, 899, 1038, 1087
Cfr10I RCCGGY 3 cut(s) 412, 601, 790
Cfr13I GGNCC 3 cut(s) 803, 830, 896
Cfr9I CCCGGG 1 cut(s) 800
Csp6I GTAC 2 cut(s) 934, 1021
CspAI ACCGGT 1 cut(s) 790
CspCI CAANNNNNGTGG 2 cut(s) 664, 699
CviAII CATG 6 cut(s) 35, 527, 827, 893, 1037, 1300
CviQI GTAC 2 cut(s) 934, 1021
DdeI CTNAG 5 cut(s) 807, 813, 819, 909, 1113
DpnI GATC 2 cut(s) 230, 1158
DpnII GATC 2 cut(s) 228, 1156
EciI GGCGGA 1 cut(s) 402
Eco130I CCWWGG 4 cut(s) 351, 941, 974, 1004
Eco147I AGGCCT 2 cut(s) 871, 1217
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 66, 768, 1252
Eco88I CYCGRG 1 cut(s) 800
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 4 cut(s) 351, 941, 974, 1004
EcoT22I ATGCAT 1 cut(s) 1264
ErhI CCWWGG 4 cut(s) 351, 941, 974, 1004
FaeI CATG 6 cut(s) 38, 530, 830, 896, 1040, 1303
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FatI CATG 6 cut(s) 34, 526, 826, 892, 1036, 1299
FauI CCCGC 1 cut(s) 906
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 1 cut(s) 174
FokI GGATG 5 cut(s) 434, 951, 1186, 1191, 1215
FspBI CTAG 1 cut(s) 483
GsaI CCCAGC 2 cut(s) 591, 1068
HaeIII GGCC 6 cut(s) 277, 805, 832, 871, 897, 1217
HapII CCGG 4 cut(s) 413, 602, 791, 801
Hin1II CATG 6 cut(s) 38, 530, 830, 896, 1040, 1303
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 3 cut(s) 443, 727, 1247
HpaII CCGG 4 cut(s) 413, 602, 791, 801
HphI GGTGA 5 cut(s) 121, 427, 588, 764, 777
Hpy166II GTNNAC 4 cut(s) 160, 175, 317, 785
Hpy188I TCNGA 4 cut(s) 46, 846, 1246, 1252
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 4 cut(s) 160, 175, 317, 785
HpyAV CCTTC 7 cut(s) 85, 118, 412, 500, 545, 743, 1193
HpyCH4III ACNGT 5 cut(s) 107, 199, 374, 989, 1079
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 9 cut(s) 117, 184, 439, 770, 865, 1036, 1130, 1262, 1293
HpyF3I CTNAG 5 cut(s) 807, 813, 819, 909, 1113
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 6 cut(s) 38, 530, 830, 896, 1040, 1303
KroI GCCGGC 1 cut(s) 601
KroNI GCCGGC 1 cut(s) 603
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 228, 1156
LmnI GCTCC 1 cut(s) 1099
LweI GCATC 2 cut(s) 757, 973
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 127, 193, 594
MalI GATC 2 cut(s) 230, 1158
MboI GATC 2 cut(s) 228, 1156
MboII GAAGA 4 cut(s) 59, 154, 472, 1258
MhlI GDGCHC 3 cut(s) 858, 930, 996
MluCI AATT 4 cut(s) 147, 292, 557, 755
MlyI GAGTC 1 cut(s) 452
Mph1103I ATGCAT 1 cut(s) 1264
MreI CGCCGGCG 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 601
MseI TTAA 1 cut(s) 560
MslI CAYNNNNRTG 3 cut(s) 108, 531, 1125
MspI CCGG 4 cut(s) 413, 602, 791, 801
MspR9I CCNGG 2 cut(s) 801, 802
Mva1269I GAATGC 1 cut(s) 1262
NaeI GCCGGC 1 cut(s) 603
NciI CCSGG 2 cut(s) 801, 802
NdeII GATC 2 cut(s) 228, 1156
NgoMIV GCCGGC 1 cut(s) 601
NlaIII CATG 6 cut(s) 38, 530, 830, 896, 1040, 1303
NlaIV GGNNCC 1 cut(s) 1197
NmuCI GTSAC 3 cut(s) 127, 193, 594
NsiI ATGCAT 1 cut(s) 1264
NspI RCATGY 1 cut(s) 1040
OliI CACNNNNGTG 1 cut(s) 108
PaeI GCATGC 1 cut(s) 1040
PaqCI CACCTGC 2 cut(s) 907, 1198
PceI AGGCCT 2 cut(s) 871, 1217
PctI GAATGC 1 cut(s) 1262
PdiI GCCGGC 1 cut(s) 603
PfeI GAWTC 2 cut(s) 727, 1247
PflMI CCANNNNNTGG 1 cut(s) 779
PinAI ACCGGT 1 cut(s) 790
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 587, 1064
PspN4I GGNNCC 1 cut(s) 1197
PspPI GGNCC 3 cut(s) 803, 830, 896
RsaI GTAC 2 cut(s) 935, 1022
RsaNI GTAC 2 cut(s) 934, 1021
RseI CAYNNNNRTG 3 cut(s) 108, 531, 1125
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 560
Sau3AI GATC 2 cut(s) 228, 1156
Sau96I GGNCC 3 cut(s) 803, 830, 896
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 801, 802
SduI GDGCHC 3 cut(s) 858, 930, 996
SfaNI GCATC 2 cut(s) 757, 973
SgrAI CRCCGGYG 1 cut(s) 601
SmaI CCCGGG 1 cut(s) 802
SmiMI CAYNNNNRTG 3 cut(s) 108, 531, 1125
SphI GCATGC 1 cut(s) 1040
SrfI GCCCGGGC 1 cut(s) 802
Sse9I AATT 4 cut(s) 147, 292, 557, 755
SseBI AGGCCT 2 cut(s) 871, 1217
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 2 cut(s) 871, 1217
StyD4I CCNGG 2 cut(s) 799, 800
StyI CCWWGG 4 cut(s) 351, 941, 974, 1004
TaaI ACNGT 5 cut(s) 107, 199, 374, 989, 1079
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 174, 765
TasI AATT 4 cut(s) 147, 292, 557, 755
TatI WGTACW 1 cut(s) 1020
TfiI GAWTC 2 cut(s) 727, 1247
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 6 cut(s) 110, 243, 688, 784, 992, 1276
TseFI GTSAC 3 cut(s) 127, 193, 594
Tsp45I GTSAC 3 cut(s) 127, 193, 594
TspDTI ATGAA 4 cut(s) 51, 438, 1058, 1290
TspMI CCCGGG 1 cut(s) 800
TspRI CASTG 6 cut(s) 110, 243, 688, 784, 992, 1276
Van91I CCANNNNNTGG 1 cut(s) 779
XceI RCATGY 1 cut(s) 1040
XmaI CCCGGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 483
ZrmI AGTACT 1 cut(s) 1022
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.