Rroxscaffold_6G00424130

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005906
Physical Location & Seq
Reverse (-)
45340354 .. 45342308
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424130.1

Sequence Viewer

Length: 1308 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCATGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACATTACACAGGTCGACTATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTTTGATCAAGACACTGTCCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGCCAATTCCAAAGACAAGAAACAAAGCAAACCCAAAGACAAAGAGAAACAGAGTGACCCAGCTGAAATCTCCGGGGAAGCAAAAGCAAACCACGGTGGCGGCGGCCGCGGAGATAAGAAAAATGGTAAAGAGCCGGTGAAGGATGAAGTTGTTGAAGTGCAAAAGTCTGGTGGGGGTAAGAAGAACTTTGAAGTTGGTAGTTCTAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTCAAACCACACGAGGGTGGCGGCAGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACAGCCGGCGGCGGCTGAAAAGCAGAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGAAAAGTGGAGGTGGTGCTGGCAATGGCAGTGGTGGTGGGGGTGGGGGTAAAAAGAAAAAGGGCAAGGGGCAGAACGGGAATGACAATGTCAATACTGCTGAAGGTGAACTGGTTGTCAATGCACCAAGCACTGGTTCACCACCGGTTCAGCCCGGGCCTCAGCCTCATGGACCGCCTAATGTTCAGTACCAAGTGCCCAATATGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGGCAGCAGCAGCAGCAGTATGAGCATCCTTACCCAAGGCAGCAGCACTATGAGCACCCTTACCCAAGGCAGCAGCAGCAGTACTTTACTCCTGCGCATGCGATGAACTACAATGCGGCACACCCCAGCATTAGCTACGGTGCGGCTCGCTATGCTCCACAACCTGTTTATTCTCAATCGCACACTTATGCACATTGGGGTTTGGGGAATGTGGTTGATCCACCAGCCTATGATTTGGACAACCTTCCAGCTGCGGTGCCATCCCATCCTCCACAGGCCTATCCACCACATCCTTGCTATTCCTCTGATTCCGATAACTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCAGTATCATGTGA

Protein Analysis

435

Amino Acids

46.65

Weight (kDa)

7.7

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 1.4e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 907
Acc16I TGCGCA 1 cut(s) 1038
Acc36I ACCTGC 1 cut(s) 907
AccB1I GGYRCC 1 cut(s) 1198
AccB7I CCANNNNNTGG 1 cut(s) 794
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 396
AclWI GGATC 1 cut(s) 1154
AcoI YGGCCR 1 cut(s) 391
AcuI CTGAAG 3 cut(s) 66, 783, 1255
AfaI GTAC 3 cut(s) 851, 935, 1025
AfiI CCNNNNNNNGG 2 cut(s) 539, 794
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 805
AgsI TTSAA 5 cut(s) 9, 91, 142, 443, 479
AjuI GAANNNNNNNTTGG 2 cut(s) 781, 813
AleI CACNNNNGTG 2 cut(s) 108, 540
AluBI AGCT 5 cut(s) 350, 591, 653, 1077, 1193
AluI AGCT 5 cut(s) 350, 591, 653, 1077, 1193
Alw21I GWGCWC 2 cut(s) 930, 999
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 6 cut(s) 275, 391, 818, 872, 898, 1218
AsiGI ACCGGT 1 cut(s) 805
AspLEI GCGC 1 cut(s) 1039
AspS9I GGNCC 3 cut(s) 818, 833, 899
AsuC2I CCSGG 3 cut(s) 361, 816, 817
AsuHPI GGTGA 4 cut(s) 121, 436, 779, 792
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 861
BanI GGYRCC 1 cut(s) 1198
BauI CACGAG 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 930, 999
BbvCI CCTCAGC 2 cut(s) 822, 909
BccI CCATC 3 cut(s) 146, 1210, 1215
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 3 cut(s) 361, 816, 817
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 907
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 3 cut(s) 361, 816, 817
Bme18I GGWCC 1 cut(s) 833
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 3 cut(s) 818, 833, 899
BmiI GGNNCC 1 cut(s) 1200
BmrFI CCNGG 3 cut(s) 361, 816, 817
BmsI GCATC 1 cut(s) 976
Bpu10I CCTNAGC 2 cut(s) 822, 909
BpuMI CCSGG 3 cut(s) 361, 816, 817
BsaBI GATNNNNATC 1 cut(s) 1281
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 7 cut(s) 360, 379, 394, 815, 941, 977, 1007
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 794
Bse118I RCCGGY 3 cut(s) 421, 610, 805
Bse1I ACTGG 5 cut(s) 589, 601, 777, 799, 881
Bse3DI GCAATG 1 cut(s) 691
Bse8I GATNNNNATC 1 cut(s) 1281
BseDI CCNNGG 7 cut(s) 360, 379, 394, 815, 941, 977, 1007
BseGI GGATG 5 cut(s) 436, 967, 1202, 1207, 1231
BseJI GATNNNNATC 1 cut(s) 1281
BseLI CCNNNNNNNGG 2 cut(s) 539, 794
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 2 cut(s) 836, 923
BseNI ACTGG 5 cut(s) 589, 601, 777, 799, 881
BseSI GKGCMC 1 cut(s) 861
BseX3I CGGCCG 1 cut(s) 391
BseYI CCCAGC 3 cut(s) 346, 596, 1067
Bsh1236I CGCG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 6 cut(s) 277, 393, 820, 874, 900, 1220
BshNI GGYRCC 1 cut(s) 1198
BshTI ACCGGT 1 cut(s) 805
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 930, 999
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 5 cut(s) 360, 422, 611, 806, 816
BslI CCNNNNNNNGG 2 cut(s) 539, 794
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 1265
BsnI GGCC 6 cut(s) 277, 393, 820, 874, 900, 1220
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 3 cut(s) 861, 930, 999
Bsp143I GATC 2 cut(s) 228, 1159
BspANI GGCC 6 cut(s) 277, 393, 820, 874, 900, 1220
BspCNI CTCAG 2 cut(s) 835, 922
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 1 cut(s) 1200
BspMI ACCTGC 1 cut(s) 907
BspPI GGATC 1 cut(s) 1154
BspT107I GGYRCC 1 cut(s) 1198
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 691
BsrFI RCCGGY 3 cut(s) 421, 610, 805
BsrI ACTGG 5 cut(s) 589, 601, 777, 799, 881
BssAI RCCGGY 3 cut(s) 421, 610, 805
BssECI CCNNGG 7 cut(s) 360, 379, 394, 815, 941, 977, 1007
BssMI GATC 2 cut(s) 228, 1159
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 3 cut(s) 941, 977, 1007
Bst2BI CACGAG 1 cut(s) 536
Bst4CI ACNGT 5 cut(s) 107, 199, 240, 383, 1082
BstC8I GCNNGC 7 cut(s) 115, 593, 612, 682, 902, 1041, 1090
BstDEI CTNAG 2 cut(s) 822, 909
BstDSI CCRYGG 2 cut(s) 379, 394
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 5 cut(s) 436, 967, 1202, 1207, 1231
BstFNI CGCG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 1039
BstKTI GATC 2 cut(s) 231, 1162
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 228, 1159
BstMCI CGRYCG 1 cut(s) 394
BstNSI RCATGY 1 cut(s) 1043
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 3 cut(s) 359, 814, 815
BstSLI GKGCMC 1 cut(s) 861
BstUI CGCG 1 cut(s) 396
BstZI CGGCCG 1 cut(s) 391
BsuRI GGCC 6 cut(s) 277, 393, 820, 874, 900, 1220
BtgI CCRYGG 2 cut(s) 379, 394
BtgZI GCGATG 1 cut(s) 1058
BtsCI GGATG 5 cut(s) 436, 967, 1202, 1207, 1231
BtsI GCAGTG 2 cut(s) 556, 697
BtsIMutI CAGTG 6 cut(s) 103, 236, 556, 697, 792, 1279
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 7 cut(s) 115, 593, 612, 682, 902, 1041, 1090
CciNI GCGGCCGC 1 cut(s) 391
CfoI GCGC 1 cut(s) 1039
Cfr10I RCCGGY 3 cut(s) 421, 610, 805
Cfr13I GGNCC 3 cut(s) 818, 833, 899
Cfr42I CCGCGG 1 cut(s) 397
Cfr9I CCCGGG 1 cut(s) 815
Csp6I GTAC 3 cut(s) 850, 934, 1024
CspAI ACCGGT 1 cut(s) 805
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CviAII CATG 5 cut(s) 35, 830, 896, 1040, 1303
CviQI GTAC 3 cut(s) 850, 934, 1024
DdeI CTNAG 2 cut(s) 822, 909
DpnI GATC 2 cut(s) 230, 1161
DpnII GATC 2 cut(s) 228, 1159
EaeI YGGCCR 1 cut(s) 391
EagI CGGCCG 1 cut(s) 391
EclXI CGGCCG 1 cut(s) 391
Eco130I CCWWGG 3 cut(s) 941, 977, 1007
Eco147I AGGCCT 2 cut(s) 874, 1220
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 833
Eco52I CGGCCG 1 cut(s) 391
Eco57I CTGAAG 3 cut(s) 66, 783, 1255
Eco88I CYCGRG 1 cut(s) 815
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 941, 977, 1007
EcoT22I ATGCAT 1 cut(s) 1267
ErhI CCWWGG 3 cut(s) 941, 977, 1007
FaeI CATG 5 cut(s) 38, 833, 899, 1043, 1306
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FatI CATG 5 cut(s) 34, 829, 895, 1039, 1302
FauI CCCGC 1 cut(s) 909
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 1 cut(s) 174
FokI GGATG 5 cut(s) 443, 954, 1189, 1194, 1218
FspBI CTAG 1 cut(s) 492
FspI TGCGCA 1 cut(s) 1038
GlaI GCGC 1 cut(s) 1038
GsaI CCCAGC 3 cut(s) 350, 600, 1071
HaeIII GGCC 6 cut(s) 277, 393, 820, 874, 900, 1220
HapII CCGG 5 cut(s) 360, 422, 611, 806, 816
HhaI GCGC 1 cut(s) 1039
Hin1II CATG 5 cut(s) 38, 833, 899, 1043, 1306
Hin6I GCGC 1 cut(s) 1037
HinP1I GCGC 1 cut(s) 1037
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 1250
HpaII CCGG 5 cut(s) 360, 422, 611, 806, 816
HphI GGTGA 4 cut(s) 121, 436, 779, 792
Hpy166II GTNNAC 4 cut(s) 160, 175, 770, 800
Hpy188I TCNGA 3 cut(s) 46, 1249, 1255
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 4 cut(s) 160, 175, 770, 800
HpyAV CCTTC 7 cut(s) 85, 118, 421, 509, 554, 758, 1196
HpyCH4III ACNGT 5 cut(s) 107, 199, 240, 383, 1082
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 117, 184, 448, 785, 868, 1133, 1265, 1296
HpyF3I CTNAG 2 cut(s) 822, 909
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 5 cut(s) 38, 833, 899, 1043, 1306
HspAI GCGC 1 cut(s) 1037
KroI GCCGGC 1 cut(s) 610
KroNI GCCGGC 1 cut(s) 612
Ksp22I TGATCA 1 cut(s) 228
KspI CCGCGG 1 cut(s) 397
Kzo9I GATC 2 cut(s) 228, 1159
LmnI GCTCC 1 cut(s) 1102
LweI GCATC 1 cut(s) 976
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 4 cut(s) 127, 193, 341, 603
MalI GATC 2 cut(s) 230, 1161
MboI GATC 2 cut(s) 228, 1159
MboII GAAGA 4 cut(s) 59, 154, 481, 1261
MhlI GDGCHC 3 cut(s) 861, 930, 999
MluCI AATT 3 cut(s) 147, 292, 566
Mph1103I ATGCAT 1 cut(s) 1267
MroNI GCCGGC 1 cut(s) 610
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 3 cut(s) 108, 540, 1128
MspA1I CMGCKG 3 cut(s) 350, 396, 1193
MspI CCGG 5 cut(s) 360, 422, 611, 806, 816
MspR9I CCNGG 3 cut(s) 361, 816, 817
Mva1269I GAATGC 1 cut(s) 1265
MvnI CGCG 1 cut(s) 396
NaeI GCCGGC 1 cut(s) 612
NciI CCSGG 3 cut(s) 361, 816, 817
NdeII GATC 2 cut(s) 228, 1159
NgoMIV GCCGGC 1 cut(s) 610
NlaIII CATG 5 cut(s) 38, 833, 899, 1043, 1306
NlaIV GGNNCC 1 cut(s) 1200
NmuCI GTSAC 4 cut(s) 127, 193, 341, 603
NotI GCGGCCGC 1 cut(s) 391
NsbI TGCGCA 1 cut(s) 1038
NsiI ATGCAT 1 cut(s) 1267
NspI RCATGY 1 cut(s) 1043
OliI CACNNNNGTG 2 cut(s) 108, 540
PaeI GCATGC 1 cut(s) 1043
PaqCI CACCTGC 1 cut(s) 907
PceI AGGCCT 2 cut(s) 874, 1220
PctI GAATGC 1 cut(s) 1265
PdiI GCCGGC 1 cut(s) 612
PfeI GAWTC 1 cut(s) 1250
PflFI GACNNNGTC 2 cut(s) 238, 749
PflMI CCANNNNNTGG 1 cut(s) 794
PinAI ACCGGT 1 cut(s) 805
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 3 cut(s) 346, 596, 1067
PspN4I GGNNCC 1 cut(s) 1200
PspPI GGNCC 3 cut(s) 818, 833, 899
PsyI GACNNNGTC 2 cut(s) 238, 749
PvuII CAGCTG 2 cut(s) 350, 1193
RsaI GTAC 3 cut(s) 851, 935, 1025
RsaNI GTAC 3 cut(s) 850, 934, 1024
RseI CAYNNNNRTG 3 cut(s) 108, 540, 1128
SacII CCGCGG 1 cut(s) 397
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 569
Sau3AI GATC 2 cut(s) 228, 1159
Sau96I GGNCC 3 cut(s) 818, 833, 899
ScaI AGTACT 1 cut(s) 1025
ScrFI CCNGG 3 cut(s) 361, 816, 817
SduI GDGCHC 3 cut(s) 861, 930, 999
SfaNI GCATC 1 cut(s) 976
Sfr303I CCGCGG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 1 cut(s) 833
SmaI CCCGGG 1 cut(s) 817
SmiMI CAYNNNNRTG 3 cut(s) 108, 540, 1128
SphI GCATGC 1 cut(s) 1043
SrfI GCCCGGGC 1 cut(s) 817
Sse9I AATT 3 cut(s) 147, 292, 566
SseBI AGGCCT 2 cut(s) 874, 1220
SspMI CTAG 1 cut(s) 492
StuI AGGCCT 2 cut(s) 874, 1220
StyD4I CCNGG 3 cut(s) 359, 814, 815
StyI CCWWGG 3 cut(s) 941, 977, 1007
TaaI ACNGT 5 cut(s) 107, 199, 240, 383, 1082
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 147, 292, 566
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 8 cut(s) 390, 393, 396, 549, 617, 620, 1061, 1088
TfiI GAWTC 1 cut(s) 1250
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 6 cut(s) 110, 243, 556, 697, 799, 1279
TseFI GTSAC 4 cut(s) 127, 193, 341, 603
Tsp45I GTSAC 4 cut(s) 127, 193, 341, 603
TspDTI ATGAA 4 cut(s) 51, 447, 1061, 1293
TspMI CCCGGG 1 cut(s) 815
TspRI CASTG 6 cut(s) 110, 243, 556, 697, 799, 1279
Tth111I GACNNNGTC 2 cut(s) 238, 749
Van91I CCANNNNNTGG 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 833
XceI RCATGY 1 cut(s) 1043
XmaI CCCGGG 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 492
ZrmI AGTACT 1 cut(s) 1025
Zsp2I ATGCAT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.