Rroxscaffold_6G00423630

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005906
Physical Location & Seq
Reverse (-)
44693636 .. 44695593
1958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423630.1

Sequence Viewer

Length: 1179 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCATGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACATTACACAGGTCGACTATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTTTGATCAAGACACTGTCCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGCCAATTCCAAAGACAAGAAACAAAGCAAACCCAAAGACAAAGAGAAACAGAGTGACCCAGCTGAAATCTCCGGGAAGCAAAAGCAAACCACGAACTTTGAAGTTGGTAGTTCTAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTCAAACCACACGAGGGTGGCGGCAGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACAGCCGGCGGCGGCTGAAAAGCAGAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGAAAAGTGGAGGTGGTGCTGGCAATGGCAGTGGTGGTGGGGGTGGGGGTGGGGGTGAACTGGTTGTCAATGCACCAAGCACTGGTTCACCACCGGTTCAGCCCGGGCCTCAGCCTCAGCCTCAGCCTCATGGACCGCCTAATGTTCAGAACCAAGTGCCCAATATGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGGCAGCAGCAGCAGCAGTATGAGCATCCTTACCCAAGGCAGCAGCACTATGAGCACCCTTACCCAAGGCAGCAGCAGCAGTACTTTACTCCTGCGCATGCGATGAACTACAATGCGGCACACCCCAGCATTAGCTACGGTGCGGCTCGCTATGCTCCACAACCTGTTTATTCTCAATCGCACACTTATGCACATTGGGGTTTGGGGAATGTGGTTGATCCACCAGCCTATGATTTGGACAACCTTCCAGCTGCGGTGCCATCCCATCCTCCACAGGCCTATCCACCACATCCTTGCTATTCCTCTGATTCCGATAACTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCAGTATCATGTGA

Protein Analysis

392

Amino Acids

42.25

Weight (kDa)

6.6

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 1.2e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 778
Acc16I TGCGCA 1 cut(s) 909
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 1 cut(s) 1069
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 174
AciI CCGC 8 cut(s) 456, 524, 527, 707, 773, 929, 956, 1067
AclWI GGATC 1 cut(s) 1025
AcuI CTGAAG 2 cut(s) 66, 1126
AfaI GTAC 2 cut(s) 806, 896
AfiI CCNNNNNNNGG 2 cut(s) 449, 653
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 664
AgsI TTSAA 4 cut(s) 9, 91, 142, 389
AjuI GAANNNNNNNTTGG 2 cut(s) 640, 672
AleI CACNNNNGTG 2 cut(s) 108, 450
AluBI AGCT 5 cut(s) 350, 501, 563, 948, 1064
AluI AGCT 5 cut(s) 350, 501, 563, 948, 1064
Alw21I GWGCWC 2 cut(s) 801, 870
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 1025
Ama87I CYCGRG 1 cut(s) 674
AoxI GGCC 5 cut(s) 275, 677, 743, 769, 1089
AsiGI ACCGGT 1 cut(s) 664
AspLEI GCGC 1 cut(s) 910
AspS9I GGNCC 3 cut(s) 677, 704, 770
AsuC2I CCSGG 3 cut(s) 361, 675, 676
AsuHPI GGTGA 3 cut(s) 121, 638, 651
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 732
BanI GGYRCC 1 cut(s) 1069
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 801, 870
BbvCI CCTCAGC 4 cut(s) 681, 687, 693, 780
BccI CCATC 3 cut(s) 146, 1081, 1086
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 3 cut(s) 361, 675, 676
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 778
BmcAI AGTACT 1 cut(s) 896
Bme1390I CCNGG 3 cut(s) 361, 675, 676
Bme18I GGWCC 1 cut(s) 704
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 3 cut(s) 677, 704, 770
BmiI GGNNCC 1 cut(s) 1071
BmrFI CCNGG 3 cut(s) 361, 675, 676
BmsI GCATC 1 cut(s) 847
Bpu10I CCTNAGC 4 cut(s) 681, 687, 693, 780
BpuMI CCSGG 3 cut(s) 361, 675, 676
BsaBI GATNNNNATC 1 cut(s) 1152
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 4 cut(s) 674, 812, 848, 878
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 653
Bse118I RCCGGY 2 cut(s) 520, 664
Bse1I ACTGG 5 cut(s) 499, 511, 636, 658, 752
Bse3DI GCAATG 1 cut(s) 601
Bse8I GATNNNNATC 1 cut(s) 1152
BseDI CCNNGG 4 cut(s) 674, 812, 848, 878
BseGI GGATG 4 cut(s) 838, 1073, 1078, 1102
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 2 cut(s) 449, 653
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 4 cut(s) 695, 701, 707, 794
BseNI ACTGG 5 cut(s) 499, 511, 636, 658, 752
BseSI GKGCMC 1 cut(s) 732
BseYI CCCAGC 3 cut(s) 346, 506, 938
BshFI GGCC 5 cut(s) 277, 679, 745, 771, 1091
BshNI GGYRCC 1 cut(s) 1069
BshTI ACCGGT 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 801, 870
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 4 cut(s) 360, 521, 665, 675
BslI CCNNNNNNNGG 2 cut(s) 449, 653
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 5 cut(s) 277, 679, 745, 771, 1091
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 674
Bsp1286I GDGCHC 3 cut(s) 732, 801, 870
Bsp143I GATC 2 cut(s) 228, 1030
BspACI CCGC 8 cut(s) 456, 524, 527, 707, 773, 929, 956, 1067
BspANI GGCC 5 cut(s) 277, 679, 745, 771, 1091
BspCNI CTCAG 4 cut(s) 694, 700, 706, 793
BspLI GGNNCC 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 1 cut(s) 1025
BspT107I GGYRCC 1 cut(s) 1069
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 601
BsrFI RCCGGY 2 cut(s) 520, 664
BsrI ACTGG 5 cut(s) 499, 511, 636, 658, 752
BssAI RCCGGY 2 cut(s) 520, 664
BssECI CCNNGG 4 cut(s) 674, 812, 848, 878
BssMI GATC 2 cut(s) 228, 1030
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 3 cut(s) 812, 848, 878
Bst2BI CACGAG 1 cut(s) 446
Bst4CI ACNGT 4 cut(s) 107, 199, 240, 953
BstC8I GCNNGC 7 cut(s) 115, 503, 522, 592, 773, 912, 961
BstDEI CTNAG 4 cut(s) 681, 687, 693, 780
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 4 cut(s) 838, 1073, 1078, 1102
BstHHI GCGC 1 cut(s) 910
BstKTI GATC 2 cut(s) 231, 1033
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 228, 1030
BstMWI GCNNNNNNNGC 9 cut(s) 507, 736, 781, 823, 826, 835, 865, 889, 965
BstNSI RCATGY 1 cut(s) 914
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 3 cut(s) 359, 673, 674
BstSLI GKGCMC 1 cut(s) 732
BsuRI GGCC 5 cut(s) 277, 679, 745, 771, 1091
BtgZI GCGATG 1 cut(s) 929
BtsCI GGATG 4 cut(s) 838, 1073, 1078, 1102
BtsI GCAGTG 2 cut(s) 466, 607
BtsIMutI CAGTG 6 cut(s) 103, 236, 466, 607, 651, 1150
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 7 cut(s) 115, 503, 522, 592, 773, 912, 961
CfoI GCGC 1 cut(s) 910
Cfr10I RCCGGY 2 cut(s) 520, 664
Cfr13I GGNCC 3 cut(s) 677, 704, 770
Cfr9I CCCGGG 1 cut(s) 674
Csp6I GTAC 2 cut(s) 805, 895
CspAI ACCGGT 1 cut(s) 664
CspCI CAANNNNNGTGG 4 cut(s) 367, 402, 583, 618
CviAII CATG 5 cut(s) 35, 701, 767, 911, 1174
CviQI GTAC 2 cut(s) 805, 895
DdeI CTNAG 4 cut(s) 681, 687, 693, 780
DpnI GATC 2 cut(s) 230, 1032
DpnII GATC 2 cut(s) 228, 1030
Eco130I CCWWGG 3 cut(s) 812, 848, 878
Eco147I AGGCCT 2 cut(s) 745, 1091
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 704
Eco57I CTGAAG 2 cut(s) 66, 1126
Eco88I CYCGRG 1 cut(s) 674
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 812, 848, 878
EcoT22I ATGCAT 1 cut(s) 1138
ErhI CCWWGG 3 cut(s) 812, 848, 878
FaeI CATG 5 cut(s) 38, 704, 770, 914, 1177
FatI CATG 5 cut(s) 34, 700, 766, 910, 1173
FauI CCCGC 1 cut(s) 780
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 1 cut(s) 174
FokI GGATG 4 cut(s) 825, 1060, 1065, 1089
FspBI CTAG 1 cut(s) 402
FspI TGCGCA 1 cut(s) 909
GlaI GCGC 1 cut(s) 909
GsaI CCCAGC 3 cut(s) 350, 510, 942
HaeIII GGCC 5 cut(s) 277, 679, 745, 771, 1091
HapII CCGG 4 cut(s) 360, 521, 665, 675
HhaI GCGC 1 cut(s) 910
Hin1II CATG 5 cut(s) 38, 704, 770, 914, 1177
Hin6I GCGC 1 cut(s) 908
HinP1I GCGC 1 cut(s) 908
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 1121
HpaII CCGG 4 cut(s) 360, 521, 665, 675
HphI GGTGA 3 cut(s) 121, 638, 651
Hpy166II GTNNAC 4 cut(s) 160, 175, 629, 659
Hpy188I TCNGA 4 cut(s) 46, 720, 1120, 1126
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 4 cut(s) 160, 175, 629, 659
HpyAV CCTTC 5 cut(s) 85, 118, 419, 464, 1067
HpyCH4III ACNGT 4 cut(s) 107, 199, 240, 953
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 7 cut(s) 117, 184, 644, 739, 1004, 1136, 1167
HpyF10VI GCNNNNNNNGC 9 cut(s) 507, 736, 781, 823, 826, 835, 865, 889, 965
HpyF3I CTNAG 4 cut(s) 681, 687, 693, 780
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 5 cut(s) 38, 704, 770, 914, 1177
HspAI GCGC 1 cut(s) 908
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 228, 1030
LmnI GCTCC 1 cut(s) 973
LweI GCATC 1 cut(s) 847
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 4 cut(s) 127, 193, 341, 513
MalI GATC 2 cut(s) 230, 1032
MboI GATC 2 cut(s) 228, 1030
MboII GAAGA 3 cut(s) 59, 154, 1132
MhlI GDGCHC 3 cut(s) 732, 801, 870
MluCI AATT 3 cut(s) 147, 292, 476
Mph1103I ATGCAT 1 cut(s) 1138
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 3 cut(s) 108, 450, 999
MspA1I CMGCKG 2 cut(s) 350, 1064
MspI CCGG 4 cut(s) 360, 521, 665, 675
MspR9I CCNGG 3 cut(s) 361, 675, 676
Mva1269I GAATGC 1 cut(s) 1136
MwoI GCNNNNNNNGC 9 cut(s) 507, 736, 781, 823, 826, 835, 865, 889, 965
NaeI GCCGGC 1 cut(s) 522
NciI CCSGG 3 cut(s) 361, 675, 676
NdeII GATC 2 cut(s) 228, 1030
NgoMIV GCCGGC 1 cut(s) 520
NlaIII CATG 5 cut(s) 38, 704, 770, 914, 1177
NlaIV GGNNCC 1 cut(s) 1071
NmuCI GTSAC 4 cut(s) 127, 193, 341, 513
NsbI TGCGCA 1 cut(s) 909
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 1 cut(s) 914
OliI CACNNNNGTG 2 cut(s) 108, 450
PaeI GCATGC 1 cut(s) 914
PaqCI CACCTGC 1 cut(s) 778
PceI AGGCCT 2 cut(s) 745, 1091
PctI GAATGC 1 cut(s) 1136
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 1121
PflFI GACNNNGTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 653
PfoI TCCNGGA 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 664
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 3 cut(s) 346, 506, 938
PspN4I GGNNCC 1 cut(s) 1071
PspPI GGNCC 3 cut(s) 677, 704, 770
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 350, 1064
RsaI GTAC 2 cut(s) 806, 896
RsaNI GTAC 2 cut(s) 805, 895
RseI CAYNNNNRTG 3 cut(s) 108, 450, 999
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 479
Sau3AI GATC 2 cut(s) 228, 1030
Sau96I GGNCC 3 cut(s) 677, 704, 770
ScaI AGTACT 1 cut(s) 896
ScrFI CCNGG 3 cut(s) 361, 675, 676
SduI GDGCHC 3 cut(s) 732, 801, 870
SfaNI GCATC 1 cut(s) 847
SinI GGWCC 1 cut(s) 704
SmaI CCCGGG 1 cut(s) 676
SmiMI CAYNNNNRTG 3 cut(s) 108, 450, 999
SphI GCATGC 1 cut(s) 914
SrfI GCCCGGGC 1 cut(s) 676
Sse9I AATT 3 cut(s) 147, 292, 476
SseBI AGGCCT 2 cut(s) 745, 1091
SsiI CCGC 8 cut(s) 456, 524, 527, 707, 773, 929, 956, 1067
SspMI CTAG 1 cut(s) 402
StuI AGGCCT 2 cut(s) 745, 1091
StyD4I CCNGG 3 cut(s) 359, 673, 674
StyI CCWWGG 3 cut(s) 812, 848, 878
TaaI ACNGT 4 cut(s) 107, 199, 240, 953
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 147, 292, 476
TatI WGTACW 1 cut(s) 894
TauI GCSGC 5 cut(s) 459, 527, 530, 932, 959
TfiI GAWTC 1 cut(s) 1121
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 6 cut(s) 110, 243, 466, 607, 658, 1150
TseFI GTSAC 4 cut(s) 127, 193, 341, 513
Tsp45I GTSAC 4 cut(s) 127, 193, 341, 513
TspDTI ATGAA 3 cut(s) 51, 932, 1164
TspMI CCCGGG 1 cut(s) 674
TspRI CASTG 6 cut(s) 110, 243, 466, 607, 658, 1150
Tth111I GACNNNGTC 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 704
XceI RCATGY 1 cut(s) 914
XmaI CCCGGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 402
ZrmI AGTACT 1 cut(s) 896
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.