Rh3AG068500

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005906
Physical Location & Seq
Forward (+)
4759872 .. 4762544
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG068500.1

Sequence Viewer

Length: 1353 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCATGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACACTATACAGATCGACGATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTTTGATCAAGACACTGACCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGACAATTCCAAAAACAAGAAACAGAGTAAACCCAAAGGCAATGAGAAACAGAGTGAAATCTCCGAGGAAGCAAAAGCAAACCGCGGCGGCGGCGGCGGCGGCGGAGATAAGAAAAAGGGTAAAGAGCCGGTGAAGGATGAAGTTGTTGAAGTGCAAGAGTCAGGTGGGGGTAAGAAGAACTTTGAAGTTGGTAGTTCCAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTTAAACCACATGAGGGTGGCGGCGGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACAGCCGGCGGCGGCTGAAAAGCAAAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGAAAAGTGGAGGTGGTGCTGGCAATGGCAGTGGTGGTGGGGGTGGGGGTAAAAAGAAAAAGGGCAAGGGGCAGAACGGGAATCACAATGTCAATGCTGCTGAAGGTGAACTGGTTGTCAATGCACCAAGCACCGGTTCACCACCGGTTCAGCCCGGGCCTCAGCCTCAGCCTCAGCCTCATGGGCCGCCTAATGTTCAGAGCCAAGTGCCCAATGTGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGACAGCAGCAGCAGTATGAGCATCCTTCCCCAAGGCAGCAGCACTATGAGCACCCTTACCCAAGGCAGCAGCACTATGAGCACCCTTACCCAATGCAGCAGCAACAGCAACAGCAACAGCACTTTACTCCTGTGCATGCGATGAACTACAATGCAGCACACTCCAGCATTAGCTACGGTGCGGCTCGCTATGCTCCACAGCCTGTTTATTCTCAGTCGCACACTTATGCACATTGGAGTTTGGGGAATGAGGTTGATCCACCAGCCTATGATTTGGACAACCTTCCGCCTGCGCCGCCGTCCCATCTTCCACAGGCCTATCCACCACATCCTTACAACTCATCCGATTCCGATACCTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCGGTATCATGTGA

Protein Analysis

450

Amino Acids

48.36

Weight (kDa)

6.86

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 4.4e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 913
Acc36I ACCTGC 1 cut(s) 913
AccII CGCG 1 cut(s) 372
AclWI GGATC 1 cut(s) 1199
AcuI CTGAAG 3 cut(s) 66, 777, 1300
AfaI GTAC 1 cut(s) 941
AfiI CCNNNNNNNGG 2 cut(s) 533, 788
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 2 cut(s) 788, 799
AgsI TTSAA 5 cut(s) 9, 91, 142, 437, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 775, 807
AleI CACNNNNGTG 1 cut(s) 108
AluBI AGCT 3 cut(s) 585, 647, 1122
AluI AGCT 3 cut(s) 585, 647, 1122
Alw21I GWGCWC 3 cut(s) 936, 1002, 1032
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 1199
Ama87I CYCGRG 1 cut(s) 809
AoxI GGCC 6 cut(s) 275, 812, 839, 878, 904, 1263
AsiGI ACCGGT 2 cut(s) 788, 799
AspLEI GCGC 1 cut(s) 1243
AspS9I GGNCC 3 cut(s) 812, 839, 905
AsuC2I CCSGG 2 cut(s) 810, 811
AsuHPI GGTGA 4 cut(s) 121, 430, 773, 786
AvaI CYCGRG 1 cut(s) 809
BaeGI GKGCMC 1 cut(s) 867
Bbv12I GWGCWC 3 cut(s) 936, 1002, 1032
BbvCI CCTCAGC 4 cut(s) 816, 822, 828, 915
BccI CCATC 2 cut(s) 146, 1260
BceAI ACGGC 1 cut(s) 1231
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 810, 811
BcoDI GTCTC 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 913
BglI GCCNNNNNGGC 1 cut(s) 838
Bme1390I CCNGG 2 cut(s) 810, 811
BmeT110I CYCGRG 1 cut(s) 809
BmgT120I GGNCC 3 cut(s) 812, 839, 905
BmrFI CCNGG 2 cut(s) 810, 811
BmsI GCATC 1 cut(s) 979
BpmI CTGGAG 1 cut(s) 1096
Bpu10I CCTNAGC 4 cut(s) 816, 822, 828, 915
BpuMI CCSGG 2 cut(s) 810, 811
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 5 cut(s) 351, 370, 809, 980, 1010
BsaWI WCCGGW 2 cut(s) 788, 799
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 788
Bse118I RCCGGY 4 cut(s) 415, 604, 788, 799
Bse1I ACTGG 4 cut(s) 583, 595, 771, 887
Bse3DI GCAATG 2 cut(s) 334, 685
Bse8I GATNNNNATC 1 cut(s) 1326
BseDI CCNNGG 5 cut(s) 351, 370, 809, 980, 1010
BseGI GGATG 4 cut(s) 430, 970, 1276, 1289
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 2 cut(s) 533, 788
BseMI GCAATG 2 cut(s) 334, 685
BseMII CTCAG 5 cut(s) 830, 836, 842, 929, 1175
BseNI ACTGG 4 cut(s) 583, 595, 771, 887
BseSI GKGCMC 1 cut(s) 867
BseYI CCCAGC 1 cut(s) 590
BsgI GTGCAG 1 cut(s) 893
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 6 cut(s) 277, 814, 841, 880, 906, 1265
BshTI ACCGGT 2 cut(s) 788, 799
BsiHKAI GWGCWC 3 cut(s) 936, 1002, 1032
BsiHKCI CYCGRG 1 cut(s) 809
BsiSI CCGG 5 cut(s) 416, 605, 789, 800, 810
BslFI GGGAC 1 cut(s) 1234
BslI CCNNNNNNNGG 2 cut(s) 533, 788
BsmAI GTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 1234
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 6 cut(s) 277, 814, 841, 880, 906, 1265
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 809
Bsp1286I GDGCHC 4 cut(s) 867, 936, 1002, 1032
Bsp143I GATC 3 cut(s) 171, 228, 1204
BspANI GGCC 6 cut(s) 277, 814, 841, 880, 906, 1265
BspCNI CTCAG 5 cut(s) 829, 835, 841, 928, 1174
BspFNI CGCG 1 cut(s) 372
BspMI ACCTGC 1 cut(s) 913
BspPI GGATC 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 334, 685
BsrFI RCCGGY 4 cut(s) 415, 604, 788, 799
BsrI ACTGG 4 cut(s) 583, 595, 771, 887
BssAI RCCGGY 4 cut(s) 415, 604, 788, 799
BssECI CCNNGG 5 cut(s) 351, 370, 809, 980, 1010
BssMI GATC 3 cut(s) 171, 228, 1204
BssT1I CCWWGG 2 cut(s) 980, 1010
Bst4CI ACNGT 3 cut(s) 107, 199, 1127
BstC8I GCNNGC 8 cut(s) 115, 587, 606, 676, 908, 1086, 1135, 1239
BstDEI CTNAG 5 cut(s) 816, 822, 828, 915, 1161
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 4 cut(s) 430, 970, 1276, 1289
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 1243
BstKTI GATC 3 cut(s) 174, 231, 1207
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 3 cut(s) 171, 228, 1204
BstNSI RCATGY 1 cut(s) 1088
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 808, 809
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 372
BsuRI GGCC 6 cut(s) 277, 814, 841, 880, 906, 1265
BtgI CCRYGG 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 1103
BtsCI GGATG 4 cut(s) 430, 970, 1276, 1289
BtsI GCAGTG 1 cut(s) 691
BtsIMutI CAGTG 4 cut(s) 103, 236, 691, 1324
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 8 cut(s) 115, 587, 606, 676, 908, 1086, 1135, 1239
CfoI GCGC 1 cut(s) 1243
Cfr10I RCCGGY 4 cut(s) 415, 604, 788, 799
Cfr13I GGNCC 3 cut(s) 812, 839, 905
Cfr42I CCGCGG 1 cut(s) 373
Cfr9I CCCGGG 1 cut(s) 809
Csp6I GTAC 1 cut(s) 940
CspAI ACCGGT 2 cut(s) 788, 799
CspCI CAANNNNNGTGG 2 cut(s) 667, 702
CviAII CATG 6 cut(s) 35, 530, 836, 902, 1085, 1348
CviQI GTAC 1 cut(s) 940
DdeI CTNAG 5 cut(s) 816, 822, 828, 915, 1161
DpnI GATC 3 cut(s) 173, 230, 1206
DpnII GATC 3 cut(s) 171, 228, 1204
EciI GGCGGA 2 cut(s) 405, 1224
Eco130I CCWWGG 2 cut(s) 980, 1010
Eco147I AGGCCT 2 cut(s) 880, 1265
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 66, 777, 1300
Eco88I CYCGRG 1 cut(s) 809
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 980, 1010
EcoT22I ATGCAT 1 cut(s) 1312
ErhI CCWWGG 2 cut(s) 980, 1010
FaeI CATG 6 cut(s) 38, 533, 839, 905, 1088, 1351
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 1234
FatI CATG 6 cut(s) 34, 529, 835, 901, 1084, 1347
FauI CCCGC 1 cut(s) 915
FbaI TGATCA 1 cut(s) 228
FokI GGATG 4 cut(s) 437, 957, 1263, 1276
GlaI GCGC 1 cut(s) 1242
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 1096
HaeIII GGCC 6 cut(s) 277, 814, 841, 880, 906, 1265
HapII CCGG 5 cut(s) 416, 605, 789, 800, 810
HhaI GCGC 1 cut(s) 1243
Hin1II CATG 6 cut(s) 38, 533, 839, 905, 1088, 1351
Hin6I GCGC 1 cut(s) 1241
HinP1I GCGC 1 cut(s) 1241
HinfI GANTC 3 cut(s) 446, 736, 1295
HpaII CCGG 5 cut(s) 416, 605, 789, 800, 810
HphI GGTGA 4 cut(s) 121, 430, 773, 786
Hpy166II GTNNAC 4 cut(s) 160, 317, 764, 794
Hpy188I TCNGA 5 cut(s) 46, 352, 855, 1294, 1300
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 4 cut(s) 160, 317, 764, 794
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 8 cut(s) 85, 118, 415, 503, 548, 752, 984, 1241
HpyCH4III ACNGT 3 cut(s) 107, 199, 1127
HpyCH4IV ACGT 1 cut(s) 261
HpyF3I CTNAG 5 cut(s) 816, 822, 828, 915, 1161
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 6 cut(s) 38, 533, 839, 905, 1088, 1351
HspAI GCGC 1 cut(s) 1241
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
Ksp22I TGATCA 1 cut(s) 228
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 3 cut(s) 171, 228, 1204
LmnI GCTCC 1 cut(s) 1147
LweI GCATC 1 cut(s) 979
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 127, 193, 597
MalI GATC 3 cut(s) 173, 230, 1206
MboI GATC 3 cut(s) 171, 228, 1204
MboII GAAGA 5 cut(s) 59, 154, 475, 1247, 1306
MhlI GDGCHC 4 cut(s) 867, 936, 1002, 1032
MluCI AATT 3 cut(s) 147, 292, 560
MlyI GAGTC 1 cut(s) 455
Mph1103I ATGCAT 1 cut(s) 1312
MroNI GCCGGC 1 cut(s) 604
MseI TTAA 2 cut(s) 522, 563
MslI CAYNNNNRTG 3 cut(s) 108, 534, 1173
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 5 cut(s) 416, 605, 789, 800, 810
MspR9I CCNGG 2 cut(s) 810, 811
Mva1269I GAATGC 1 cut(s) 1310
MvnI CGCG 1 cut(s) 372
NaeI GCCGGC 1 cut(s) 606
NciI CCSGG 2 cut(s) 810, 811
NdeII GATC 3 cut(s) 171, 228, 1204
NgoMIV GCCGGC 1 cut(s) 604
NlaIII CATG 6 cut(s) 38, 533, 839, 905, 1088, 1351
NmuCI GTSAC 3 cut(s) 127, 193, 597
NsiI ATGCAT 1 cut(s) 1312
NspI RCATGY 1 cut(s) 1088
OliI CACNNNNGTG 1 cut(s) 108
PaeI GCATGC 1 cut(s) 1088
PaqCI CACCTGC 1 cut(s) 913
PceI AGGCCT 2 cut(s) 880, 1265
PctI GAATGC 1 cut(s) 1310
PdiI GCCGGC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 736, 1295
PinAI ACCGGT 2 cut(s) 788, 799
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 590
PspPI GGNCC 3 cut(s) 812, 839, 905
RsaI GTAC 1 cut(s) 941
RsaNI GTAC 1 cut(s) 940
RseI CAYNNNNRTG 3 cut(s) 108, 534, 1173
SacII CCGCGG 1 cut(s) 373
SaqAI TTAA 2 cut(s) 522, 563
Sau3AI GATC 3 cut(s) 171, 228, 1204
Sau96I GGNCC 3 cut(s) 812, 839, 905
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 2 cut(s) 810, 811
SduI GDGCHC 4 cut(s) 867, 936, 1002, 1032
SfaNI GCATC 1 cut(s) 979
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SmaI CCCGGG 1 cut(s) 811
SmiMI CAYNNNNRTG 3 cut(s) 108, 534, 1173
SphI GCATGC 1 cut(s) 1088
SrfI GCCCGGGC 1 cut(s) 811
Sse9I AATT 3 cut(s) 147, 292, 560
SseBI AGGCCT 2 cut(s) 880, 1265
StuI AGGCCT 2 cut(s) 880, 1265
StyD4I CCNGG 2 cut(s) 808, 809
StyI CCWWGG 2 cut(s) 980, 1010
TaaI ACNGT 3 cut(s) 107, 199, 1127
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 147, 292, 560
TfiI GAWTC 2 cut(s) 736, 1295
Tru1I TTAA 2 cut(s) 522, 563
Tru9I TTAA 2 cut(s) 522, 563
TscAI CASTG 4 cut(s) 110, 243, 691, 1324
TseFI GTSAC 3 cut(s) 127, 193, 597
Tsp45I GTSAC 3 cut(s) 127, 193, 597
TspDTI ATGAA 4 cut(s) 51, 441, 1106, 1338
TspMI CCCGGG 1 cut(s) 809
TspRI CASTG 4 cut(s) 110, 243, 691, 1324
XceI RCATGY 1 cut(s) 1088
XmaI CCCGGG 1 cut(s) 809
Zsp2I ATGCAT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.