Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003079

Physical Location & Seq

Genomic Coordinates Reverse (-)

32338209 .. 32341259

Gene Structure 3051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1255300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGAGCTGCTTTCCGTGTTTCGGCTCACAGAGAAGTAAGAGATCAAATAGCAACAAGAGATTAGAGAATGCCTCCTCTCCTCCTCCTATCGCGCAAGAATTGAAGAAACAAAAACCCGCAGAGGCCACCGGGGGAGTTAACCCAAAAGATATTAATGCAAAGACTTTCACTTTCCGTGAGCTCGCCACTGCAACAAAGAATTTCCGGCAGGAATGTCTTTTGGGGGAAGGTGGATTTGGAAGAGTCTATAAGGGAACCCTTCAGTCGAGCGGGCAGGTGGTGGCGGTGAAGCAACTAGACAGACATGGAACGCAAGGGAACGAATTTCTTGGGGACGTTTTGACGCTAAGCCTCCTACACCATCCAAATCTGGTTAACCTAATTGGATATTGTGCTGATGGAGATCAAAGACTTTTGGTGTATGAATTCATATCCGGGGGTTCTCTAGATGATCGTGTTCTGGGCAATGTACCAGATAACAAGCCATTAGATTGGTACACCCGAGTTAAAATAGCTTATGGTGCTGCTTTGGGACTGGAGTACTTGCACGAAAAGGCCAATCCCTCTGTTGTCTATCGAGATTTGAAATCTTCGAACATTTTGTTAGACGAAGAATTCAATCCAAAGCTCTCAGATGTTGGACTTGCCCAGCTTGGCCCTGAGGGAGATAAGATGCATGGACCATCAAGATTGATGGGAACCTATGGTTTCTGTGCTCCTGAGTATTCTAGATCAGGTGAAGTCACAATGAAGTCGGATGTGTACAGTTTCGGAGTTATTCTTCTGGAGCTCATTACCGGAAGAAGAGCTGTCGACACTACAAGGTCAAATGATGAGCAAAATCTAGTATCTTGGGCACAACCCTTATTCAGGGACCCAAAAAAGTATCCTGATATGGCAGATCCTCTTCTTAACAAGCAGTTCCCGGAAAAGGATCTAAATCAAGCTGTTGCAATGGCATCCATGTGTTTGCAAGAGGAGGCAGCAGTCCGTCCCTTCATGAGTGACGTTGTGACTACTCTAAGTTTTCTTTCCACAACTCCTCCTCCTGAGGCTGTCCCTGCTTCTCTTCCAGCTGATCCAACCCCAACTGAAAAACGTGAAGAGAACAATGATGAATCAGAATGTGTTTCCGAATATTCAGAAGACGATAATTCTGATGCTGCAGGAGATTATGATGATGAAGACCAGGAGGCTTCTGATCAGGAGAGTATCAGAAATTCAAAGAGCAACCGCCGTAGTGTTGACATTTCAGAAGGTGAAGGTAGCGAAGATGAACGCCGAGTAACTGTTACAGAATCCAAAGAATGGCATTCATTTACAAGGGGCAGCATGATATCACGGAACGACAGTGTTAATTCAAGTGACCAGAGCAGCAAGGGTGGTAATAATCTGGATGGTAATTCAAACCAAGTGAGCAGCAGTCAATCCAAATATGCAAGTGTTTCTTCTAGAAGCAGCAGCAGAGGGTCAACAGAAGAAACCGTTTCCACTAGCAGAAAGAAAAAAGCATCACAAAAAGAAACTGCTACTGTCACATTAGGCGGCAATAGCAGTACCGAGGAAAAATCATCAGAAGGCGGAAGTGTTTGCACAGATGATCAGGATCAGGAAAACAGAAGCAGCAAAGTACCACAACTACCACCAGTACTGAAAGATGGAAAAGATTCTGTAGTCCAACACAGAAACAACAACGGATCGCAGGATTTTAAATCATCGAAAAAATGTCAATCCAATCACTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009856 GO:0009987 GO:0010183 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0030427 GO:0032501 GO:0032502 GO:0035838 GO:0036211 GO:0038023 GO:0040011 GO:0042221 GO:0042330 GO:0042995 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044463 GO:0044464 GO:0044706 GO:0048856 GO:0048868 GO:0050789 GO:0050794 GO:0050896 GO:0050918 GO:0051286 GO:0051704 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0090404 GO:0090406 GO:0120025 GO:0120038 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

582

Amino Acids

63.64

Weight (kDa)

5.12

Isoelectric Point (pI)

57.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	70 - 337	1.3e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	71 - 285	1.6e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003079)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07070 AT3G24790 AT3G24790 AT4G13190 AT4G13190 AT4G13190 AT4G13190 AT4G13190
fragaria_vesca	FvH4_2g19200 FvH4_6g01120
malus_domestica	MD04G1236200.v1.1 MD05G1176700.v1.1 MD10G1165400.v1.1 MD12G1255300.v1.1
prunus_persica	Prupe.6G356200_v2.0.a1 Prupe.8G203800_v2.0.a1
pyrus_communis	pycom05g16290 pycom12g23400
rosa_chinensis	RchiOBHm_Chr3g0448651 RchiOBHm_Chr6g0284981
rosa_laevigata	RLG00000012693 RLG00000025882
rosa_multiflora	Rmu_sc0003116.1_g000035
rosa_roxburghii	Rroxscaffold_6G00426920 Rroxscaffold_7G00183870 Rroxscaffold_7G00184150
rosa_rugosa	Rorug02G0615000
rosa_samantha	Rh3AG013800 Rh3BG013600 Rh3CG012700 Rh3DG014400 Rh6AG281200 Rh6BG281900 Rh6CG283600 Rh6DG276700
rosa_wichuraiana	Rw3G001020 Rw6G024170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	265
Acc36I	ACCTGC	1 cut(s)	265
AccB7I	CCANNNNNTGG	1 cut(s)	1308
AccBSI	CCGCTC	1 cut(s)	270
AccI	GTMKAC	1 cut(s)	813
AccII	CGCG	1 cut(s)	92
AciI	CCGC	6 cut(s)	117, 270, 284, 1234, 1545, 1581
AclWI	GGATC	5 cut(s)	896, 942, 1073, 1614, 1705
AcsI	RAATTY	5 cut(s)	199, 323, 425, 614, 1219
AcuI	CTGAAG	1 cut(s)	245
AfaI	GTAC	7 cut(s)	471, 497, 542, 764, 1558, 1632, 1650
AfiI	CCNNNNNNNGG	4 cut(s)	20, 870, 931, 1308
AgsI	TTSAA	6 cut(s)	103, 586, 619, 1224, 1362, 1407
AjnI	CCWGG	1 cut(s)	1188
AjuI	GAANNNNNNNTTGG	2 cut(s)	219, 251
AluBI	AGCT	9 cut(s)	6, 181, 515, 628, 652, 790, 809, 947, 1076
AluI	AGCT	9 cut(s)	6, 181, 515, 628, 652, 790, 809, 947, 1076
Alw21I	GWGCWC	3 cut(s)	183, 718, 792
AlwI	GGATC	5 cut(s)	896, 942, 1073, 1614, 1705
Ama87I	CYCGRG	1 cut(s)	501
AoxI	GGCC	3 cut(s)	123, 555, 655
ApoI	RAATTY	5 cut(s)	199, 323, 425, 614, 1219
AseI	ATTAAT	1 cut(s)	153
Asp700I	GAANNNNTTC	1 cut(s)	1666
AspLEI	GCGC	1 cut(s)	94
AspS9I	GGNCC	3 cut(s)	656, 680, 874
AsuC2I	CCSGG	3 cut(s)	130, 436, 926
AsuHPI	GGTGA	3 cut(s)	298, 749, 1271
AsuII	TTCGAA	1 cut(s)	593
AvaI	CYCGRG	1 cut(s)	501
AvaII	GGWCC	2 cut(s)	680, 874
AxyI	CCTNAGG	2 cut(s)	660, 1050
BaeGI	GKGCMC	1 cut(s)	859
BanII	GRGCYC	2 cut(s)	183, 792
BbsI	GAAGAC	2 cut(s)	1152, 1191
Bbv12I	GWGCWC	3 cut(s)	183, 718, 792
BbvI	GCAGC	9 cut(s)	511, 995, 1150, 1341, 1386, 1431, 1470, 1473, 1635
BccI	CCATC	6 cut(s)	369, 392, 688, 691, 1391, 1652
BceAI	ACGGC	1 cut(s)	1221
BciT130I	CCWGG	1 cut(s)	1190
BciVI	GTATCC	1 cut(s)	897
BclI	TGATCA	2 cut(s)	1201, 1600
BcnI	CCSGG	3 cut(s)	130, 436, 926
BfaI	CTAG	6 cut(s)	296, 446, 729, 845, 1452, 1494
BfmI	CTRYAG	2 cut(s)	1164, 1671
BfuAI	ACCTGC	1 cut(s)	265
BfuI	GTATCC	1 cut(s)	897
BlpI	GCTNAGC	1 cut(s)	347
BmcAI	AGTACT	2 cut(s)	542, 1650
Bme1390I	CCNGG	4 cut(s)	130, 436, 926, 1190
Bme18I	GGWCC	2 cut(s)	680, 874
BmeT110I	CYCGRG	1 cut(s)	501
BmgT120I	GGNCC	3 cut(s)	656, 680, 874
BmiI	GGNNCC	4 cut(s)	256, 700, 875, 876
BmrFI	CCNGG	4 cut(s)	130, 436, 926, 1190
BmsI	GCATC	4 cut(s)	663, 968, 1150, 1520
BpiI	GAAGAC	2 cut(s)	1152, 1191
BplI	GAGNNNNNCTC	2 cut(s)	56, 88
BpmI	CTGGAG	2 cut(s)	557, 806
Bpu1102I	GCTNAGC	1 cut(s)	347
Bpu14I	TTCGAA	1 cut(s)	593
BpuMI	CCSGG	3 cut(s)	130, 436, 926
BsaBI	GATNNNNATC	3 cut(s)	402, 939, 1605
BsaJI	CCNNGG	3 cut(s)	129, 435, 1560
BsaWI	WCCGGW	1 cut(s)	797
BsaXI	ACNNNNNCTCC	4 cut(s)	1027, 1030, 1057, 1060
Bsc4I	CCNNNNNNNGG	4 cut(s)	20, 870, 931, 1308
Bse1I	ACTGG	2 cut(s)	540, 1646
Bse21I	CCTNAGG	2 cut(s)	660, 1050
Bse3DI	GCAATG	2 cut(s)	472, 960
Bse8I	GATNNNNATC	3 cut(s)	402, 939, 1605
BseBI	CCWGG	1 cut(s)	1190
BseDI	CCNNGG	3 cut(s)	129, 435, 1560
BseGI	GGATG	4 cut(s)	361, 763, 959, 1402
BseJI	GATNNNNATC	3 cut(s)	402, 939, 1605
BseLI	CCNNNNNNNGG	4 cut(s)	20, 870, 931, 1308
BseMI	GCAATG	2 cut(s)	472, 960
BseMII	CTCAG	4 cut(s)	645, 651, 711, 1041
BseNI	ACTGG	2 cut(s)	540, 1646
BseRI	GAGGAG	6 cut(s)	64, 69, 72, 992, 1032, 1035
BseSI	GKGCMC	1 cut(s)	859
BseXI	GCAGC	9 cut(s)	511, 995, 1150, 1341, 1386, 1431, 1470, 1473, 1635
BseYI	CCCAGC	1 cut(s)	648
Bsh1236I	CGCG	1 cut(s)	92
BshFI	GGCC	3 cut(s)	125, 557, 657
BsiHKAI	GWGCWC	3 cut(s)	183, 718, 792
BsiHKCI	CYCGRG	1 cut(s)	501
BsiSI	CCGG	5 cut(s)	129, 205, 435, 798, 926
BslFI	GGGAC	5 cut(s)	347, 546, 887, 978, 1043
BslI	CCNNNNNNNGG	4 cut(s)	20, 870, 931, 1308
BsmFI	GGGAC	5 cut(s)	347, 546, 887, 978, 1043
BsmI	GAATGC	2 cut(s)	73, 1312
BsnI	GGCC	3 cut(s)	125, 557, 657
BsoBI	CYCGRG	1 cut(s)	501
Bsp119I	TTCGAA	1 cut(s)	593
Bsp1286I	GDGCHC	4 cut(s)	183, 718, 792, 859
Bsp1407I	TGTACA	1 cut(s)	762
Bsp1720I	GCTNAGC	1 cut(s)	347
BspACI	CCGC	6 cut(s)	117, 270, 284, 1234, 1545, 1581
BspANI	GGCC	3 cut(s)	125, 557, 657
BspCNI	CTCAG	4 cut(s)	644, 652, 712, 1042
BspFNI	CGCG	1 cut(s)	92
BspHI	TCATGA	1 cut(s)	999
BspLI	GGNNCC	4 cut(s)	256, 700, 875, 876
BspMAI	CTGCAG	1 cut(s)	1168
BspMI	ACCTGC	1 cut(s)	265
BspPI	GGATC	5 cut(s)	896, 942, 1073, 1614, 1705
BspQI	GCTCTTC	1 cut(s)	799
BspT104I	TTCGAA	1 cut(s)	593
BsrBI	CCGCTC	1 cut(s)	270
BsrDI	GCAATG	2 cut(s)	472, 960
BsrGI	TGTACA	1 cut(s)	762
BsrI	ACTGG	2 cut(s)	540, 1646
BssECI	CCNNGG	3 cut(s)	129, 435, 1560
Bst2UI	CCWGG	1 cut(s)	1190
Bst4CI	ACNGT	5 cut(s)	767, 1291, 1352, 1486, 1534
Bst6I	CTCTTC	5 cut(s)	235, 799, 912, 1074, 1098
BstAUI	TGTACA	1 cut(s)	762
BstBI	TTCGAA	1 cut(s)	593
BstC8I	GCNNGC	2 cut(s)	183, 272
BstDEI	CTNAG	6 cut(s)	347, 631, 660, 720, 1022, 1050
BstENI	CCTNNNNNAGG	1 cut(s)	868
BstF5I	GGATG	4 cut(s)	361, 763, 959, 1402
BstFNI	CGCG	1 cut(s)	92
BstHHI	GCGC	1 cut(s)	94
BstMWI	GCNNNNNNNGC	3 cut(s)	521, 1061, 1551
BstNI	CCWGG	1 cut(s)	1190
BstSCI	CCNGG	4 cut(s)	128, 434, 924, 1188
BstSFI	CTRYAG	2 cut(s)	1164, 1671
BstSLI	GKGCMC	1 cut(s)	859
BstUI	CGCG	1 cut(s)	92
BstV1I	GCAGC	9 cut(s)	511, 995, 1150, 1341, 1386, 1431, 1470, 1473, 1635
BstV2I	GAAGAC	2 cut(s)	1152, 1191
BstX2I	RGATCY	2 cut(s)	901, 934
BstXI	CCANNNNNNTGG	1 cut(s)	492
BstYI	RGATCY	2 cut(s)	901, 934
Bsu36I	CCTNAGG	2 cut(s)	660, 1050
BsuI	GTATCC	1 cut(s)	897
BsuRI	GGCC	3 cut(s)	125, 557, 657
BtsCI	GGATG	4 cut(s)	361, 763, 959, 1402
BtsI	GCAGTG	1 cut(s)	186
BtsIMutI	CAGTG	2 cut(s)	186, 1357
BveI	ACCTGC	1 cut(s)	265
Cac8I	GCNNGC	2 cut(s)	183, 272
CciI	TCATGA	1 cut(s)	999
CfoI	GCGC	1 cut(s)	94
Cfr13I	GGNCC	3 cut(s)	656, 680, 874
CseI	GACGC	1 cut(s)	352
Csp6I	GTAC	7 cut(s)	470, 496, 541, 763, 1557, 1631, 1649
CviAII	CATG	5 cut(s)	305, 677, 964, 1000, 1333
CviQI	GTAC	7 cut(s)	470, 496, 541, 763, 1557, 1631, 1649
DdeI	CTNAG	6 cut(s)	347, 631, 660, 720, 1022, 1050
DraI	TTTAAA	1 cut(s)	1711
Eam1104I	CTCTTC	5 cut(s)	235, 799, 912, 1074, 1098
EarI	CTCTTC	5 cut(s)	235, 799, 912, 1074, 1098
EciI	GGCGGA	1 cut(s)	1596
Ecl136II	GAGCTC	2 cut(s)	181, 790
Eco24I	GRGCYC	2 cut(s)	183, 792
Eco32I	GATATC	1 cut(s)	1338
Eco47I	GGWCC	2 cut(s)	680, 874
Eco53kI	GAGCTC	2 cut(s)	181, 790
Eco57I	CTGAAG	1 cut(s)	245
Eco81I	CCTNAGG	2 cut(s)	660, 1050
Eco88I	CYCGRG	1 cut(s)	501
EcoICRI	GAGCTC	2 cut(s)	181, 790
EcoNI	CCTNNNNNAGG	1 cut(s)	868
EcoO109I	RGGNCCY	1 cut(s)	874
EcoRI	GAATTC	2 cut(s)	425, 614
EcoRII	CCWGG	1 cut(s)	1188
EcoRV	GATATC	1 cut(s)	1338
EcoT22I	ATGCAT	1 cut(s)	678
EcoT38I	GRGCYC	2 cut(s)	183, 792
FaeI	CATG	5 cut(s)	308, 680, 967, 1003, 1336
FalI	AAGNNNNNCTT	2 cut(s)	1432, 1464
FaqI	GGGAC	5 cut(s)	347, 546, 887, 978, 1043
FatI	CATG	5 cut(s)	304, 676, 963, 999, 1332
FauI	CCCGC	2 cut(s)	124, 263
FbaI	TGATCA	2 cut(s)	1201, 1600
FblI	GTMKAC	1 cut(s)	813
FokI	GGATG	4 cut(s)	348, 770, 946, 1409
FriOI	GRGCYC	2 cut(s)	183, 792
FspBI	CTAG	6 cut(s)	296, 446, 729, 845, 1452, 1494
GlaI	GCGC	1 cut(s)	93
GsaI	CCCAGC	1 cut(s)	652
GsuI	CTGGAG	2 cut(s)	557, 806
HaeIII	GGCC	3 cut(s)	125, 557, 657
HapII	CCGG	5 cut(s)	129, 205, 435, 798, 926
HgaI	GACGC	1 cut(s)	352
HhaI	GCGC	1 cut(s)	94
Hin1II	CATG	5 cut(s)	308, 680, 967, 1003, 1336
Hin6I	GCGC	1 cut(s)	92
HinP1I	GCGC	1 cut(s)	92
HincII	GTYRAC	5 cut(s)	139, 376, 814, 1246, 1473
HindII	GTYRAC	5 cut(s)	139, 376, 814, 1246, 1473
HinfI	GANTC	4 cut(s)	243, 1118, 1298, 1667
HpaI	GTTAAC	2 cut(s)	139, 376
HpaII	CCGG	5 cut(s)	129, 205, 435, 798, 926
HphI	GGTGA	3 cut(s)	298, 749, 1271
Hpy166II	GTNNAC	7 cut(s)	139, 376, 498, 763, 814, 1246, 1473
Hpy8I	GTNNAC	7 cut(s)	139, 376, 498, 763, 814, 1246, 1473
HpyAV	CCTTC	6 cut(s)	221, 269, 1006, 1250, 1256, 1571
HpyCH4III	ACNGT	5 cut(s)	767, 1291, 1352, 1486, 1534
HpyCH4IV	ACGT	3 cut(s)	336, 1008, 1099
HpyCH4V	TGCA	9 cut(s)	158, 191, 547, 676, 953, 973, 1166, 1439, 1593
HpyF10VI	GCNNNNNNNGC	3 cut(s)	521, 1061, 1551
HpyF3I	CTNAG	6 cut(s)	347, 631, 660, 720, 1022, 1050
HpySE526I	ACGT	3 cut(s)	336, 1008, 1099
Hsp92II	CATG	5 cut(s)	308, 680, 967, 1003, 1336
HspAI	GCGC	1 cut(s)	92
KflI	GGGWCCC	1 cut(s)	874
Ksp22I	TGATCA	2 cut(s)	1201, 1600
KspAI	GTTAAC	2 cut(s)	139, 376
LguI	GCTCTTC	1 cut(s)	799
LmnI	GCTCC	2 cut(s)	721, 787
Lsp1109I	GCAGC	9 cut(s)	511, 995, 1150, 1341, 1386, 1431, 1470, 1473, 1635
LweI	GCATC	4 cut(s)	663, 968, 1150, 1520
MaeI	CTAG	6 cut(s)	296, 446, 729, 845, 1452, 1494
MaeII	ACGT	3 cut(s)	336, 1008, 1099
MaeIII	GTNAC	7 cut(s)	742, 1004, 1012, 1285, 1291, 1364, 1534
MbiI	CCGCTC	1 cut(s)	270
MflI	RGATCY	2 cut(s)	901, 934
MhlI	GDGCHC	4 cut(s)	183, 718, 792, 859
MlyI	GAGTC	1 cut(s)	252
MmeI	TCCRAC	4 cut(s)	619, 735, 1106, 1702
Mph1103I	ATGCAT	1 cut(s)	678
MroXI	GAANNNNTTC	1 cut(s)	1666
MseI	TTAA	7 cut(s)	138, 153, 375, 507, 912, 1356, 1710
MslI	CAYNNNNRTG	1 cut(s)	964
MspA1I	CMGCKG	1 cut(s)	1076
MspI	CCGG	5 cut(s)	129, 205, 435, 798, 926
MspR9I	CCNGG	4 cut(s)	130, 436, 926, 1190
Mva1269I	GAATGC	2 cut(s)	73, 1312
MvaI	CCWGG	1 cut(s)	1190
MvnI	CGCG	1 cut(s)	92
MwoI	GCNNNNNNNGC	3 cut(s)	521, 1061, 1551
NciI	CCSGG	3 cut(s)	130, 436, 926
NlaIII	CATG	5 cut(s)	308, 680, 967, 1003, 1336
NlaIV	GGNNCC	4 cut(s)	256, 700, 875, 876
NmeAIII	GCCGAG	1 cut(s)	1307
NmuCI	GTSAC	5 cut(s)	742, 1004, 1012, 1364, 1534
NsiI	ATGCAT	1 cut(s)	678
NspV	TTCGAA	1 cut(s)	593
PagI	TCATGA	1 cut(s)	999
PaqCI	CACCTGC	1 cut(s)	265
PciSI	GCTCTTC	1 cut(s)	799
PctI	GAATGC	2 cut(s)	73, 1312
PdmI	GAANNNNTTC	1 cut(s)	1666
PfeI	GAWTC	3 cut(s)	1118, 1298, 1667
PflMI	CCANNNNNTGG	1 cut(s)	1308
PfoI	TCCNGGA	1 cut(s)	924
PleI	GAGTC	1 cut(s)	251
PpsI	GAGTC	1 cut(s)	251
PpuMI	RGGWCCY	1 cut(s)	874
PshBI	ATTAAT	1 cut(s)	153
Psp124BI	GAGCTC	2 cut(s)	183, 792
Psp5II	RGGWCCY	1 cut(s)	874
Psp6I	CCWGG	1 cut(s)	1188
PspFI	CCCAGC	1 cut(s)	648
PspGI	CCWGG	1 cut(s)	1188
PspN4I	GGNNCC	4 cut(s)	256, 700, 875, 876
PspPI	GGNCC	3 cut(s)	656, 680, 874
PspPPI	RGGWCCY	1 cut(s)	874
PstI	CTGCAG	1 cut(s)	1168
PsuI	RGATCY	2 cut(s)	901, 934
PvuII	CAGCTG	1 cut(s)	1076
RsaI	GTAC	7 cut(s)	471, 497, 542, 764, 1558, 1632, 1650
RsaNI	GTAC	7 cut(s)	470, 496, 541, 763, 1557, 1631, 1649
RseI	CAYNNNNRTG	1 cut(s)	964
SacI	GAGCTC	2 cut(s)	183, 792
SalI	GTCGAC	1 cut(s)	812
SapI	GCTCTTC	1 cut(s)	799
SaqAI	TTAA	7 cut(s)	138, 153, 375, 507, 912, 1356, 1710
Sau96I	GGNCC	3 cut(s)	656, 680, 874
ScaI	AGTACT	2 cut(s)	542, 1650
SchI	GAGTC	1 cut(s)	252
ScrFI	CCNGG	4 cut(s)	130, 436, 926, 1190
SduI	GDGCHC	4 cut(s)	183, 718, 792, 859
SfaNI	GCATC	4 cut(s)	663, 968, 1150, 1520
SfcI	CTRYAG	2 cut(s)	1164, 1671
SfuI	TTCGAA	1 cut(s)	593
SinI	GGWCC	2 cut(s)	680, 874
SmiMI	CAYNNNNRTG	1 cut(s)	964
SsiI	CCGC	6 cut(s)	117, 270, 284, 1234, 1545, 1581
SspI	AATATT	1 cut(s)	1139
SspMI	CTAG	6 cut(s)	296, 446, 729, 845, 1452, 1494
SstI	GAGCTC	2 cut(s)	183, 792
StyD4I	CCNGG	4 cut(s)	128, 434, 924, 1188
TaaI	ACNGT	5 cut(s)	767, 1291, 1352, 1486, 1534
TaiI	ACGT	3 cut(s)	339, 1011, 1102
TaqI	TCGA	5 cut(s)	266, 577, 593, 813, 1718
TatI	WGTACW	3 cut(s)	540, 762, 1648
TauI	GCSGC	1 cut(s)	1548
TfiI	GAWTC	3 cut(s)	1118, 1298, 1667
Tru1I	TTAA	7 cut(s)	138, 153, 375, 507, 912, 1356, 1710
Tru9I	TTAA	7 cut(s)	138, 153, 375, 507, 912, 1356, 1710
TscAI	CASTG	2 cut(s)	193, 1357
TseFI	GTSAC	5 cut(s)	742, 1004, 1012, 1364, 1534
Tsp45I	GTSAC	5 cut(s)	742, 1004, 1012, 1364, 1534
TspDTI	ATGAA	8 cut(s)	418, 438, 764, 988, 1131, 1197, 1290, 1305
TspGWI	ACGGA	4 cut(s)	164, 980, 1357, 1710
TspRI	CASTG	2 cut(s)	193, 1357
Van91I	CCANNNNNTGG	1 cut(s)	1308
VpaK11BI	GGWCC	2 cut(s)	680, 874
VspI	ATTAAT	1 cut(s)	153
XagI	CCTNNNNNAGG	1 cut(s)	868
XapI	RAATTY	5 cut(s)	199, 323, 425, 614, 1219
XbaI	TCTAGA	3 cut(s)	445, 728, 1451
XmiI	GTMKAC	1 cut(s)	813
XmnI	GAANNNNTTC	1 cut(s)	1666
XspI	CTAG	6 cut(s)	296, 446, 729, 845, 1452, 1494
ZrmI	AGTACT	2 cut(s)	542, 1650
Zsp2I	ATGCAT	1 cut(s)	678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.