MD13G1060600.v1.1

Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005151
Physical Location & Seq
Forward (+)
4267570 .. 4269624
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1060600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGTTAGGAAGTGCGCGTGTTCGGCTGAACCGGTGGCAGCAGGCGGCTGTGGCGGTCGGTTCGGCTGTGGGCGCATTGCTGGACCCAAGAAGAGCTGATCTCATAGCAGCTCTTGGAGAGACAACTGGGAAACCTGCATTTGAAAGAGTTCTTGAAAGAATGACGAGGAGCCCTGAAGGCAGGGCCATACTGTTGGAGAAACCCCGTGTAATATCTGTAAACGTGGGTCATGCATGGGATCTACCAGCGAACACATTTGGTGCTGCCTATGCAAAGTTCATGGGATCTAGGAACTTCTCGCCAGATGATCGACCACCAGTGCGGTTCATGGAGACAGATGAGCTTGCATATGTGGCCATGAGGGCTCGCGAGGTGCATGACTTTTGGCACACCCTTTTTGGCCTTCCAACGAACTTGATGGGGGAGTCGGCCCTCAAGGTAATAGAGTTTGAGCAAATGTACCTTCCCATGTGCCTGATGTCCGTCATAGGGGGCTCAGTGAGATTCAACGAGAAGCAAAGGAAATTGTTCTTTCAACACTACTTCCCTTGGGCCATCCAAGCTGGAATGCAGTGCACGGATCTCATGTGCGTGTATTACGAGCGGCACTTTCACGAGGATTTGGAGGACGTTCGGAGAAAGTGGGGTATAATTCCTGCCCCTGCTCCTCCTAAACAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Coq4

Protein Analysis

228

Amino Acids

25.8

Weight (kDa)

8.81

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coq4 PF05019 6 - 221 4.9e-84 Coenzyme Q (ubiquinone) biosynthesis protein Coq4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 604
AccII CGCG 2 cut(s) 16, 369
AciI CCGC 4 cut(s) 44, 53, 322, 604
AclWI GGATC 3 cut(s) 246, 292, 588
AcoI YGGCCR 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 195
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 489
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 143, 155, 508, 536
AluBI AGCT 4 cut(s) 95, 110, 343, 563
AluI AGCT 4 cut(s) 95, 110, 343, 563
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 2 cut(s) 113, 326
Alw44I GTGCAC 1 cut(s) 574
AlwI GGATC 3 cut(s) 246, 292, 588
AoxI GGCC 5 cut(s) 183, 354, 400, 429, 552
ApaLI GTGCAC 1 cut(s) 574
ApeKI GCWGC 3 cut(s) 37, 107, 263
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 2 cut(s) 147, 527
AspLEI GCGC 2 cut(s) 16, 74
AspS9I GGNCC 4 cut(s) 82, 183, 430, 552
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 578
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 3 cut(s) 173, 367, 497
BauI CACGAG 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 578
BbvI GCAGC 3 cut(s) 49, 119, 250
BccI CCATC 2 cut(s) 412, 563
BcoDI GTCTC 2 cut(s) 113, 326
BfaI CTAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 142
BglI GCCNNNNNGGC 2 cut(s) 177, 362
BisI GCNGC 5 cut(s) 38, 45, 108, 264, 605
BlsI GCNGC 5 cut(s) 39, 46, 109, 265, 606
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 82, 183, 430, 552
BmiI GGNNCC 2 cut(s) 84, 170
BmrI ACTGGG 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 84, 116
BpuEI CTTGAG 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 130, 317
Bse3DI GCAATG 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 1 cut(s) 555
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 2 cut(s) 130, 317
BseRI GAGGAG 2 cut(s) 181, 657
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 3 cut(s) 49, 119, 250
Bsh1236I CGCG 2 cut(s) 16, 369
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 5 cut(s) 185, 356, 402, 431, 554
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 578
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 2 cut(s) 113, 326
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 5 cut(s) 185, 356, 402, 431, 554
Bsp1286I GDGCHC 4 cut(s) 173, 367, 497, 578
Bsp143I GATC 5 cut(s) 97, 238, 284, 307, 580
Bsp68I TCGCGA 1 cut(s) 369
BspACI CCGC 4 cut(s) 44, 53, 322, 604
BspANI GGCC 5 cut(s) 185, 356, 402, 431, 554
BspCNI CTCAG 1 cut(s) 509
BspFNI CGCG 2 cut(s) 16, 369
BspLI GGNNCC 2 cut(s) 84, 170
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 3 cut(s) 246, 292, 588
BspQI GCTCTTC 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 604
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 2 cut(s) 130, 317
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 5 cut(s) 97, 238, 284, 307, 580
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 548
Bst2BI CACGAG 1 cut(s) 614
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 42, 345, 367
BstDEI CTNAG 1 cut(s) 496
BstF5I GGATG 1 cut(s) 555
BstFNI CGCG 2 cut(s) 16, 369
BstHHI GCGC 2 cut(s) 16, 74
BstKTI GATC 5 cut(s) 100, 241, 287, 310, 583
BstMAI GTCTC 2 cut(s) 113, 326
BstMBI GATC 5 cut(s) 97, 238, 284, 307, 580
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 8 cut(s) 22, 50, 71, 177, 269, 353, 362, 560
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 2 cut(s) 16, 369
BstV1I GCAGC 3 cut(s) 49, 119, 250
BstX2I RGATCY 3 cut(s) 238, 284, 580
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 3 cut(s) 238, 284, 580
BsuRI GGCC 5 cut(s) 185, 356, 402, 431, 554
BtsCI GGATG 1 cut(s) 555
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 3 cut(s) 324, 504, 578
BtuMI TCGCGA 1 cut(s) 369
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 42, 345, 367
CfoI GCGC 2 cut(s) 16, 74
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 82, 183, 430, 552
Csp6I GTAC 1 cut(s) 460
CspAI ACCGGT 1 cut(s) 30
CviAII CATG 8 cut(s) 230, 234, 280, 328, 358, 377, 469, 586
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 1 cut(s) 496
DpnI GATC 5 cut(s) 99, 240, 286, 309, 582
DpnII GATC 5 cut(s) 97, 238, 284, 307, 580
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 548
Eco24I GRGCYC 3 cut(s) 173, 367, 497
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 3 cut(s) 173, 367, 497
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 8 cut(s) 233, 237, 283, 331, 361, 380, 472, 589
FatI CATG 8 cut(s) 229, 233, 279, 327, 357, 376, 468, 585
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 5 cut(s) 38, 45, 108, 264, 605
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 3 cut(s) 173, 367, 497
Fsp4HI GCNGC 5 cut(s) 38, 45, 108, 264, 605
FspBI CTAG 1 cut(s) 288
GlaI GCGC 2 cut(s) 15, 73
GluI GCNGC 5 cut(s) 38, 45, 108, 264, 605
HaeIII GGCC 5 cut(s) 185, 356, 402, 431, 554
HapII CCGG 1 cut(s) 31
HhaI GCGC 2 cut(s) 16, 74
Hin1II CATG 8 cut(s) 233, 237, 283, 331, 361, 380, 472, 589
Hin6I GCGC 2 cut(s) 14, 72
HinP1I GCGC 2 cut(s) 14, 72
HinfI GANTC 2 cut(s) 425, 504
HpaII CCGG 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 220, 576
Hpy188I TCNGA 1 cut(s) 636
Hpy188III TCNNGA 3 cut(s) 152, 368, 614
Hpy8I GTNNAC 2 cut(s) 220, 576
HpyAV CCTTC 3 cut(s) 170, 413, 473
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 2 cut(s) 222, 630
HpyCH4V TGCA 7 cut(s) 137, 233, 272, 347, 376, 571, 576
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 50, 71, 177, 269, 353, 362, 560
HpyF3I CTNAG 1 cut(s) 496
HpySE526I ACGT 2 cut(s) 222, 630
Hsp92II CATG 8 cut(s) 233, 237, 283, 331, 361, 380, 472, 589
HspAI GCGC 2 cut(s) 14, 72
Kzo9I GATC 5 cut(s) 97, 238, 284, 307, 580
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 2 cut(s) 168, 670
Lsp1109I GCAGC 3 cut(s) 49, 119, 250
MaeI CTAG 1 cut(s) 288
MaeII ACGT 2 cut(s) 222, 630
MalI GATC 5 cut(s) 99, 240, 286, 309, 582
MbiI CCGCTC 1 cut(s) 604
MboI GATC 5 cut(s) 97, 238, 284, 307, 580
MboII GAAGA 1 cut(s) 102
MflI RGATCY 3 cut(s) 238, 284, 580
MhlI GDGCHC 4 cut(s) 173, 367, 497, 578
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 524, 651
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 2 cut(s) 174, 431
MnlI CCTC 7 cut(s) 159, 354, 364, 443, 610, 619, 678
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 2 cut(s) 147, 527
MscI TGGCCA 1 cut(s) 356
MslI CAYNNNNRTG 1 cut(s) 590
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 573
MvnI CGCG 2 cut(s) 16, 369
MwoI GCNNNNNNNGC 8 cut(s) 22, 50, 71, 177, 269, 353, 362, 560
NdeI CATATG 1 cut(s) 349
NdeII GATC 5 cut(s) 97, 238, 284, 307, 580
NlaIII CATG 8 cut(s) 233, 237, 283, 331, 361, 380, 472, 589
NlaIV GGNNCC 2 cut(s) 84, 170
NruI TCGCGA 1 cut(s) 369
NsiI ATGCAT 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 85
PctI GAATGC 1 cut(s) 573
PdmI GAANNNNTTC 2 cut(s) 147, 527
PfeI GAWTC 1 cut(s) 504
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 5 cut(s) 39, 46, 109, 265, 606
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 84, 170
PspPI GGNCC 4 cut(s) 82, 183, 430, 552
PsuI RGATCY 3 cut(s) 238, 284, 580
RruI TCGCGA 1 cut(s) 369
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 85
SatI GCNGC 5 cut(s) 38, 45, 108, 264, 605
Sau3AI GATC 5 cut(s) 97, 238, 284, 307, 580
Sau96I GGNCC 4 cut(s) 82, 183, 430, 552
SchI GAGTC 1 cut(s) 434
SduI GDGCHC 4 cut(s) 173, 367, 497, 578
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 590
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 2 cut(s) 524, 651
SsiI CCGC 4 cut(s) 44, 53, 322, 604
SspMI CTAG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 2 cut(s) 225, 633
TaqI TCGA 1 cut(s) 310
TasI AATT 2 cut(s) 524, 651
TauI GCSGC 2 cut(s) 47, 607
TfiI GAWTC 1 cut(s) 504
TscAI CASTG 3 cut(s) 324, 504, 578
TseI GCWGC 3 cut(s) 37, 107, 263
TspDTI ATGAA 2 cut(s) 268, 316
TspGWI ACGGA 2 cut(s) 472, 593
TspRI CASTG 3 cut(s) 324, 504, 578
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 82
XmnI GAANNNNTTC 2 cut(s) 147, 527
XspI CTAG 1 cut(s) 288
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.