MD16G1059800.v1.1

Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005151
Physical Location & Seq
Forward (+)
4304999 .. 4306360
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1059800.v1.1.491

Sequence Viewer

Length: 684 bp
ATGTTAGGAAGTGCTCGTGTTCGGCTGAACCGGTGGCAGCAGGTGGCTGTTGCGGTCGGTTCAGCTGCGGGTGCATTGCTGGACCCAAGCAGAGCTGATCTCATTGCAGCTCTTGGAGAGACTACTGGGAAGCCTGCATTTGAGAGAGTTCTCGAAAGAATGAAGAGGAGCCCTGAAGGCAGAGCCATACTGTTGGAGCAACCCCGTGTAATATCTGTAAATGTGGGTTATGCATGGGATCTACCAGAAAACACATTTGGCGCCACCTATGCAAAGTTCATGGGATCCAGGAACTTCTCGCCAGATGATCGACCGCCTGTGCGGTTCATGGAGACAGATGAGCTTCCATATGTGGCCATGAGGGCTCGGGAGGTGCACGACTTCTGGCACACGCTTTTTGGCCTTCCGACGAACTTGATGGGGGAGTCAGCACTGAAGGTAATAGAGTTTGAGCAAATGTACCTTCCCATGTGCTTGATGTCCGTCATAGGGGGATCGGCGAGATTCAACGGTAAGCAAAGGAAATTGTTCCTTCAACACTACTTCCCTTGGGCCGTCCGAGCTGGAATGCAGTGCACAGATCTAATGTGCGTGTATTACGAGCAGCACTTTCACGAGGATTTGGATTATGTTCGGAGAAAGTGGGGTATAATTCCTGCCCCTGCTCCTCCTAAACAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Coq4

Protein Analysis

228

Amino Acids

25.78

Weight (kDa)

8.77

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coq4 PF05019 6 - 221 4.3e-81 Coenzyme Q (ubiquinone) biosynthesis protein Coq4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccB1I GGYRCC 1 cut(s) 260
AciI CCGC 4 cut(s) 53, 68, 314, 322
AclWI GGATC 4 cut(s) 246, 279, 292, 502
AcoI YGGCCR 1 cut(s) 354
AcuI CTGAAG 2 cut(s) 195, 455
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 489
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 508, 536
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 5 cut(s) 65, 95, 110, 343, 563
AluI AGCT 5 cut(s) 65, 95, 110, 343, 563
Alw21I GWGCWC 3 cut(s) 16, 378, 578
Alw26I GTCTC 2 cut(s) 113, 326
Alw44I GTGCAC 2 cut(s) 374, 574
AlwI GGATC 4 cut(s) 246, 279, 292, 502
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 3 cut(s) 354, 400, 552
ApaLI GTGCAC 2 cut(s) 374, 574
ApeKI GCWGC 4 cut(s) 37, 65, 107, 604
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 2 cut(s) 82, 552
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 2 cut(s) 378, 578
BalI TGGCCA 1 cut(s) 356
BamHI GGATCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 173, 367
BauI CACGAG 2 cut(s) 15, 614
Bbv12I GWGCWC 3 cut(s) 16, 378, 578
BbvI GCAGC 4 cut(s) 49, 52, 119, 616
BccI CCATC 1 cut(s) 412
BceAI ACGGC 1 cut(s) 539
BciT130I CCWGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 113, 326
BfoI RGCGCY 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 31
BglI GCCNNNNNGGC 2 cut(s) 177, 362
BglII AGATCT 1 cut(s) 580
BisI GCNGC 4 cut(s) 38, 66, 108, 605
BlsI GCNGC 4 cut(s) 39, 67, 109, 606
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 2 cut(s) 82, 552
BmiI GGNNCC 4 cut(s) 84, 170, 262, 286
BmrFI CCNGG 1 cut(s) 289
BmrI ACTGGG 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 84, 116
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 2 cut(s) 74, 102
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 548
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 2 cut(s) 74, 102
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 2 cut(s) 181, 657
BseSI GKGCMC 2 cut(s) 378, 578
BseXI GCAGC 4 cut(s) 49, 52, 119, 616
Bsh1285I CGRYCG 2 cut(s) 57, 314
BshFI GGCC 3 cut(s) 356, 402, 554
BshNI GGYRCC 1 cut(s) 260
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 2 cut(s) 57, 314
BsiHKAI GWGCWC 3 cut(s) 16, 378, 578
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 2 cut(s) 113, 326
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 3 cut(s) 356, 402, 554
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 5 cut(s) 16, 173, 367, 378, 578
Bsp143I GATC 6 cut(s) 97, 238, 284, 307, 494, 580
BspACI CCGC 4 cut(s) 53, 68, 314, 322
BspANI GGCC 3 cut(s) 356, 402, 554
BspLI GGNNCC 4 cut(s) 84, 170, 262, 286
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 4 cut(s) 246, 279, 292, 502
BspT107I GGYRCC 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 74, 102
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 6 cut(s) 97, 238, 284, 307, 494, 580
BssNI GRCGYC 1 cut(s) 261
BssSI CACGAG 2 cut(s) 15, 614
BssT1I CCWWGG 1 cut(s) 548
Bst2BI CACGAG 2 cut(s) 15, 614
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 2 cut(s) 192, 512
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 6 cut(s) 100, 241, 287, 310, 497, 583
BstMAI GTCTC 2 cut(s) 113, 326
BstMBI GATC 6 cut(s) 97, 238, 284, 307, 494, 580
BstMCI CGRYCG 2 cut(s) 57, 314
BstMWI GCNNNNNNNGC 5 cut(s) 71, 177, 269, 362, 560
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 287
BstSLI GKGCMC 2 cut(s) 378, 578
BstV1I GCAGC 4 cut(s) 49, 52, 119, 616
BstX2I RGATCY 3 cut(s) 238, 284, 580
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 3 cut(s) 238, 284, 580
BsuRI GGCC 3 cut(s) 356, 402, 554
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 2 cut(s) 431, 578
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 82, 552
Csp6I GTAC 1 cut(s) 460
CspAI ACCGGT 1 cut(s) 30
CviAII CATG 5 cut(s) 234, 280, 328, 358, 469
CviQI GTAC 1 cut(s) 460
DinI GGCGCC 1 cut(s) 262
DpnI GATC 6 cut(s) 99, 240, 286, 309, 496, 582
DpnII GATC 6 cut(s) 97, 238, 284, 307, 494, 580
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 548
Eco24I GRGCYC 2 cut(s) 173, 367
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 195, 455
Eco88I CYCGRG 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 2 cut(s) 173, 367
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 5 cut(s) 237, 283, 331, 361, 472
FatI CATG 5 cut(s) 233, 279, 327, 357, 468
FauI CCCGC 1 cut(s) 61
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 4 cut(s) 38, 66, 108, 605
FriOI GRGCYC 2 cut(s) 173, 367
Fsp4HI GCNGC 4 cut(s) 38, 66, 108, 605
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 38, 66, 108, 605
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 3 cut(s) 356, 402, 554
HapII CCGG 1 cut(s) 31
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 5 cut(s) 237, 283, 331, 361, 472
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HinfI GANTC 2 cut(s) 425, 504
HpaII CCGG 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 376, 576
Hpy188I TCNGA 3 cut(s) 408, 560, 636
Hpy188III TCNNGA 3 cut(s) 152, 368, 614
Hpy8I GTNNAC 2 cut(s) 376, 576
Hpy99I CGWCG 1 cut(s) 412
HpyAV CCTTC 5 cut(s) 170, 413, 430, 473, 542
HpyCH4III ACNGT 2 cut(s) 192, 512
HpyCH4V TGCA 8 cut(s) 74, 107, 137, 233, 272, 376, 571, 576
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 177, 269, 362, 560
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 5 cut(s) 237, 283, 331, 361, 472
HspAI GCGC 1 cut(s) 261
KasI GGCGCC 1 cut(s) 260
Kzo9I GATC 6 cut(s) 97, 238, 284, 307, 494, 580
LmnI GCTCC 3 cut(s) 168, 196, 670
Lsp1109I GCAGC 4 cut(s) 49, 52, 119, 616
MalI GATC 6 cut(s) 99, 240, 286, 309, 496, 582
MboI GATC 6 cut(s) 97, 238, 284, 307, 494, 580
MboII GAAGA 1 cut(s) 175
MflI RGATCY 3 cut(s) 238, 284, 580
MhlI GDGCHC 5 cut(s) 16, 173, 367, 378, 578
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 524, 651
MluNI TGGCCA 1 cut(s) 356
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 2 cut(s) 174, 431
MnlI CCTC 5 cut(s) 159, 354, 364, 610, 678
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 527
MscI TGGCCA 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 289
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 5 cut(s) 71, 177, 269, 362, 560
NarI GGCGCC 1 cut(s) 261
NdeI CATATG 1 cut(s) 349
NdeII GATC 6 cut(s) 97, 238, 284, 307, 494, 580
NlaIII CATG 5 cut(s) 237, 283, 331, 361, 472
NlaIV GGNNCC 4 cut(s) 84, 170, 262, 286
NsiI ATGCAT 1 cut(s) 235
PaqCI CACCTGC 1 cut(s) 31
PctI GAATGC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 1 cut(s) 504
PfoI TCCNGGA 1 cut(s) 287
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 4 cut(s) 39, 67, 109, 606
PleI GAGTC 1 cut(s) 433
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 4 cut(s) 84, 170, 262, 286
PspPI GGNCC 2 cut(s) 82, 552
PsuI RGATCY 3 cut(s) 238, 284, 580
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SatI GCNGC 4 cut(s) 38, 66, 108, 605
Sau3AI GATC 6 cut(s) 97, 238, 284, 307, 494, 580
Sau96I GGNCC 2 cut(s) 82, 552
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 5 cut(s) 16, 173, 367, 378, 578
SfoI GGCGCC 1 cut(s) 262
SinI GGWCC 1 cut(s) 82
Sse9I AATT 2 cut(s) 524, 651
SsiI CCGC 4 cut(s) 53, 68, 314, 322
SspDI GGCGCC 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 2 cut(s) 192, 512
TaqI TCGA 2 cut(s) 153, 310
TasI AATT 2 cut(s) 524, 651
TfiI GAWTC 1 cut(s) 504
TscAI CASTG 2 cut(s) 438, 578
TseI GCWGC 4 cut(s) 37, 65, 107, 604
TspDTI ATGAA 3 cut(s) 176, 268, 316
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 2 cut(s) 438, 578
VneI GTGCAC 2 cut(s) 374, 574
VpaK11BI GGWCC 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 527
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.