MD13G1063100.v1.1

prohibitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007435
Physical Location & Seq
Reverse (-)
4374244 .. 4377533
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1063100.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGATCCAAACCAGCAGAGACCAGGAATTCCACCAAATCGCCCTCAGCCACCACCTCAAGGCGGCGGCGGCGACTCCTCGGCCATTCTCTTTGTCCTCTTCGCTTTCCTCGCCATTTTTTTCGCCGTGACCGTTCTTCCAACATCATCAACTATATCAATTCTGCACCAAGTCCCAGAAGGTCATGTTGGGGTATATTGGAGAGGAGGCGCTCTTCTGAAGACAATTACAGATCCAGGTTTTCATCTCAAGCTACCTCTGGTCACCCATTTTGAACCCGTTCAAGTGACCCTGCAGACTGATCAGGTAAGGGATATTCCATGTGGTACAAAAGGGGGTGTCATGATCAATTTTGAGAAGATTGAGGTCGTAAATCGGCTTCATAAGGAATTTGTGTACGAGACGCTTCTCAATTATGGCGTGCAATATGACAATACATGGATATATGACAAAATTCATCATGAGATCAATCAGTTCTGCAGCTCACATTCTCTTCAAGAAGTGTATATTGATGTGTTCGATCAGATTGATGAACAGATGAAAGAAGCCCTTCAAGGTGACTGTACCCGCTACGCTCCAGGCATTGAAATCATCAATGTCCGTGTTACAAAGCCAACCATCCCGGAGAGTATAAGACGTAACTTTGAGCAAATGGAGGAGGAGCGCACCAAGGTCTTAATTGCTATAGAGAGACAGAGAGTAGTGGAGAAAGAGGCAGAAACCAAGAAGAAGATGGCTATCAGCGAGGCTGAGAAGAACGCCAATGTCAGTAAGATTCTCATGGAACAAAAGTTGACGGAGAAGGACAGTGCCAGGAGGCAGCAGGAGATTGACAATCAGATGTACACAGCTCGGGAAATGAGCCTTGCGGATGCTGATTTCTATAAAGTCATGAAGGAAGCTGAAGCAAACAAGTTGAAGCTTACTCCACAGTTTCTTGAGCTTAAATTCATCGACGCCATTGCTGATAATACTAAGATTTTCTTCGGAGACAAGGTTCCCAATATGATTCTGGATCAGAGGTTGCTGGGGAACTTCTTACAGGTGCCGACAGAGGTATCGAAAGAAGTGTCCGTGGAAGCTAACTCAGAAGGTGGTGAGGACAATCCAGAAGTCCAAACGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

376

Amino Acids

42.58

Weight (kDa)

5.17

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 57 - 238 9.3e-29 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1045
AciI CCGC 5 cut(s) 63, 66, 69, 568, 869
AclWI GGATC 3 cut(s) 12, 227, 1023
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 4 cut(s) 27, 389, 453, 947
AcuI CTGAAG 2 cut(s) 239, 924
AcyI GRCGYC 1 cut(s) 957
AfaI GTAC 4 cut(s) 328, 398, 565, 845
AfiI CCNNNNNNNGG 2 cut(s) 59, 62
AgsI TTSAA 6 cut(s) 275, 284, 497, 554, 587, 919
AjnI CCWGG 4 cut(s) 22, 235, 577, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AloI GAACNNNNNNTCC 2 cut(s) 981, 1013
AluBI AGCT 7 cut(s) 253, 483, 851, 902, 922, 943, 1082
AluI AGCT 7 cut(s) 253, 483, 851, 902, 922, 943, 1082
Alw26I GTCTC 4 cut(s) 13, 395, 685, 984
AlwI GGATC 3 cut(s) 12, 227, 1023
Ama87I CYCGRG 1 cut(s) 852
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 480, 820
ApoI RAATTY 4 cut(s) 27, 389, 453, 947
ArsI GACNNNNNNTTYG 2 cut(s) 1055, 1087
Asp700I GAANNNNTTC 2 cut(s) 279, 549
AspLEI GCGC 2 cut(s) 212, 666
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 3 cut(s) 256, 569, 1109
AvaI CYCGRG 1 cut(s) 852
BaeI ACNNNNGTAYC 2 cut(s) 1041, 1074
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 1045
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
BbsI GAAGAC 1 cut(s) 227
BbvCI CCTCAGC 1 cut(s) 45
BbvI GCAGC 2 cut(s) 492, 832
BccI CCATC 2 cut(s) 626, 727
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 4 cut(s) 24, 237, 579, 814
BclI TGATCA 2 cut(s) 301, 345
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 4 cut(s) 13, 395, 685, 984
BfmI CTRYAG 3 cut(s) 293, 478, 684
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 5 cut(s) 64, 67, 70, 481, 821
BlsI GCNGC 5 cut(s) 65, 68, 71, 482, 822
Bme1390I CCNGG 5 cut(s) 24, 237, 579, 623, 814
BmeT110I CYCGRG 1 cut(s) 852
BmiI GGNNCC 3 cut(s) 6, 999, 1047
BmrFI CCNGG 5 cut(s) 24, 237, 579, 623, 814
BmsI GCATC 1 cut(s) 862
BpiI GAAGAC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 45
BpuEI CTTGAG 3 cut(s) 42, 233, 959
BpuMI CCSGG 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 737
BsaHI GRCGYC 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 3 cut(s) 78, 669, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 981, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 62
Bse3DI GCAATG 1 cut(s) 960
Bse8I GATNNNNATC 1 cut(s) 737
BseBI CCWGG 4 cut(s) 24, 237, 579, 814
BseDI CCNNGG 3 cut(s) 78, 669, 1074
BseGI GGATG 2 cut(s) 618, 877
BseJI GATNNNNATC 1 cut(s) 737
BseLI CCNNNNNNNGG 2 cut(s) 59, 62
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 3 cut(s) 59, 741, 1101
BseRI GAGGAG 4 cut(s) 67, 219, 671, 674
BseXI GCAGC 2 cut(s) 492, 832
BseYI CCCAGC 1 cut(s) 1027
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 1045
BsiHKCI CYCGRG 1 cut(s) 852
BsiSI CCGG 1 cut(s) 623
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 59, 62
BsmAI GTCTC 4 cut(s) 13, 395, 685, 984
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 852
Bsp1407I TGTACA 1 cut(s) 843
Bsp143I GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
BspACI CCGC 5 cut(s) 63, 66, 69, 568, 869
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 3 cut(s) 58, 742, 1100
BspHI TCATGA 3 cut(s) 342, 460, 891
BspLI GGNNCC 3 cut(s) 6, 999, 1047
BspMAI CTGCAG 2 cut(s) 297, 482
BspPI GGATC 3 cut(s) 12, 227, 1023
BspQI GCTCTTC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 1045
BspTNI GGTCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 960
BsrGI TGTACA 1 cut(s) 843
BssECI CCNNGG 3 cut(s) 78, 669, 1074
BssMI GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
BssNI GRCGYC 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 669
Bst2UI CCWGG 4 cut(s) 24, 237, 579, 814
Bst4CI ACNGT 5 cut(s) 133, 563, 809, 933, 1123
Bst6I CTCTTC 3 cut(s) 104, 219, 498
BstACI GRCGYC 1 cut(s) 957
BstAUI TGTACA 1 cut(s) 843
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 4 cut(s) 45, 750, 975, 1087
BstDSI CCRYGG 1 cut(s) 1074
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 2 cut(s) 618, 877
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 212, 666
BstKTI GATC 7 cut(s) 7, 235, 304, 348, 468, 523, 1018
BstMAI GTCTC 4 cut(s) 13, 395, 685, 984
BstMBI GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
BstMWI GCNNNNNNNGC 2 cut(s) 69, 110
BstNI CCWGG 4 cut(s) 24, 237, 579, 814
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 5 cut(s) 22, 235, 577, 621, 812
BstSFI CTRYAG 3 cut(s) 293, 478, 684
BstV1I GCAGC 2 cut(s) 492, 832
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 2 cut(s) 4, 232
BstYI RGATCY 2 cut(s) 4, 232
BsuRI GGCC 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 1074
BtsCI GGATG 2 cut(s) 618, 877
BtsIMutI CAGTG 1 cut(s) 814
Cac8I GCNNGC 1 cut(s) 422
CciI TCATGA 3 cut(s) 342, 460, 891
CfoI GCGC 2 cut(s) 212, 666
CseI GACGC 2 cut(s) 412, 965
Csp6I GTAC 4 cut(s) 327, 397, 564, 844
CviAII CATG 7 cut(s) 185, 321, 343, 438, 461, 781, 892
CviQI GTAC 4 cut(s) 327, 397, 564, 844
DdeI CTNAG 4 cut(s) 45, 750, 975, 1087
DpnI GATC 7 cut(s) 6, 234, 303, 347, 467, 522, 1017
DpnII GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 104, 219, 498
EarI CTCTTC 3 cut(s) 104, 219, 498
Eco130I CCWWGG 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 239, 924
Eco88I CYCGRG 1 cut(s) 852
Eco91I GGTNACC 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 4 cut(s) 22, 235, 577, 812
EcoT14I CCWWGG 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 7 cut(s) 188, 324, 346, 441, 464, 784, 895
FalI AAGNNNNNCTT 4 cut(s) 534, 566, 968, 1000
FaqI GGGAC 1 cut(s) 158
FatI CATG 7 cut(s) 184, 320, 342, 437, 460, 780, 891
FauI CCCGC 1 cut(s) 575
FbaI TGATCA 2 cut(s) 301, 345
Fnu4HI GCNGC 5 cut(s) 64, 67, 70, 481, 821
FokI GGATG 2 cut(s) 605, 884
Fsp4HI GCNGC 5 cut(s) 64, 67, 70, 481, 821
GlaI GCGC 2 cut(s) 211, 665
GluI GCNGC 5 cut(s) 64, 67, 70, 481, 821
GsaI CCCAGC 1 cut(s) 1031
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 623
HgaI GACGC 2 cut(s) 412, 965
HhaI GCGC 2 cut(s) 212, 666
Hin1I GRCGYC 1 cut(s) 957
Hin1II CATG 7 cut(s) 188, 324, 346, 441, 464, 784, 895
Hin6I GCGC 2 cut(s) 210, 664
HinP1I GCGC 2 cut(s) 210, 664
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 3 cut(s) 74, 775, 1009
HpaII CCGG 1 cut(s) 623
HphI GGTGA 3 cut(s) 256, 569, 1109
Hpy166II GTNNAC 3 cut(s) 397, 795, 846
Hpy188I TCNGA 7 cut(s) 219, 525, 840, 989, 1020, 1090, 1127
Hpy188III TCNNGA 8 cut(s) 343, 461, 497, 854, 892, 938, 1013, 1109
Hpy8I GTNNAC 3 cut(s) 397, 795, 846
Hpy99I CGWCG 1 cut(s) 959
HpyAV CCTTC 5 cut(s) 173, 560, 796, 889, 1085
HpyCH4III ACNGT 5 cut(s) 133, 563, 809, 933, 1123
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 4 cut(s) 166, 295, 424, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 110
HpyF3I CTNAG 4 cut(s) 45, 750, 975, 1087
HpySE526I ACGT 1 cut(s) 637
Hsp92I GRCGYC 1 cut(s) 957
Hsp92II CATG 7 cut(s) 188, 324, 346, 441, 464, 784, 895
HspAI GCGC 2 cut(s) 210, 664
Ksp22I TGATCA 2 cut(s) 301, 345
Kzo9I GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 580, 661
Lsp1109I GCAGC 2 cut(s) 492, 832
LweI GCATC 1 cut(s) 862
MaeII ACGT 1 cut(s) 637
MaeIII GTNAC 6 cut(s) 127, 262, 286, 557, 604, 638
MalI GATC 7 cut(s) 6, 234, 303, 347, 467, 522, 1017
MboI GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
MflI RGATCY 2 cut(s) 4, 232
MluCI AATT 9 cut(s) 27, 159, 225, 349, 389, 412, 453, 678, 947
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 164
MroXI GAANNNNTTC 2 cut(s) 279, 549
MseI TTAA 2 cut(s) 677, 945
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 5 cut(s) 24, 237, 579, 623, 814
MvaI CCWGG 4 cut(s) 24, 237, 579, 814
MwoI GCNNNNNNNGC 2 cut(s) 69, 110
NciI CCSGG 1 cut(s) 623
NdeII GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
NlaIII CATG 7 cut(s) 188, 324, 346, 441, 464, 784, 895
NlaIV GGNNCC 3 cut(s) 6, 999, 1047
NmeAIII GCCGAG 1 cut(s) 59
NmuCI GTSAC 4 cut(s) 127, 262, 286, 557
PagI TCATGA 3 cut(s) 342, 460, 891
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 279, 549
PfeI GAWTC 2 cut(s) 775, 1009
PfoI TCCNGGA 1 cut(s) 621
PkrI GCNGC 5 cut(s) 65, 68, 71, 482, 822
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 22, 235, 577, 812
PspEI GGTNACC 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 1027
PspGI CCWGG 4 cut(s) 22, 235, 577, 812
PspN4I GGNNCC 3 cut(s) 6, 999, 1047
PstI CTGCAG 2 cut(s) 297, 482
PsuI RGATCY 2 cut(s) 4, 232
RsaI GTAC 4 cut(s) 328, 398, 565, 845
RsaNI GTAC 4 cut(s) 327, 397, 564, 844
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 677, 945
SatI GCNGC 5 cut(s) 64, 67, 70, 481, 821
Sau3AI GATC 7 cut(s) 4, 232, 301, 345, 465, 520, 1015
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 5 cut(s) 24, 237, 579, 623, 814
SfaNI GCATC 1 cut(s) 862
SfcI CTRYAG 3 cut(s) 293, 478, 684
SmlI CTYRAG 3 cut(s) 57, 248, 938
SmoI CTYRAG 3 cut(s) 57, 248, 938
Sse9I AATT 9 cut(s) 27, 159, 225, 349, 389, 412, 453, 678, 947
SsiI CCGC 5 cut(s) 63, 66, 69, 568, 869
StyD4I CCNGG 5 cut(s) 22, 235, 577, 621, 812
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 5 cut(s) 133, 563, 809, 933, 1123
TaiI ACGT 1 cut(s) 640
TaqI TCGA 3 cut(s) 519, 954, 1061
TasI AATT 9 cut(s) 27, 159, 225, 349, 389, 412, 453, 678, 947
TatI WGTACW 1 cut(s) 843
TauI GCSGC 3 cut(s) 66, 69, 72
TfiI GAWTC 2 cut(s) 775, 1009
Tru1I TTAA 2 cut(s) 677, 945
Tru9I TTAA 2 cut(s) 677, 945
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 4 cut(s) 127, 262, 286, 557
TseI GCWGC 2 cut(s) 480, 820
Tsp45I GTSAC 4 cut(s) 127, 262, 286, 557
TspDTI ATGAA 7 cut(s) 233, 371, 446, 546, 554, 908, 940
TspGWI ACGGA 3 cut(s) 590, 812, 1063
TspRI CASTG 1 cut(s) 814
XapI RAATTY 4 cut(s) 27, 389, 453, 947
XcmI CCANNNNNNNNNTGG 2 cut(s) 730, 1009
XmnI GAANNNNTTC 2 cut(s) 279, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.