MD16G1061600.v1.1

prohibitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007435
Physical Location & Seq
Reverse (-)
4387826 .. 4390991
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1061600.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGATCCAAACCAGCTGAGACCAGGAGCTCCACAAAATCGCCCTCAGCCACCACCTCAAGGCGGCGGCGATTCCTCTGCCATTTTCTTTGTCTTCTTCGCTTTCCTCGCCATTTTCGCCGTGACGGTTCTTCCAACATCAACAACTCTATCCATTCTGCACCAAGTCCCAGAAGGTCATGTTGGGGTATATTGGAGAGGAGGCGCTCTTCTAAAGACAATTACAGATCCAGGTTTTCATCTCAAGTTGCCTCTGGTCACCCATTTTGAACCAGTTCAAGTGACCCTGCAGACTGATCAGGTGAGGGATATTCCATGTGGTACAAAAGGGGGTGTCATGATCAATTTTGAGAAGATTGAGGTCGTAAACAGGCTTCGTAAGGATTTTGTGTATGAGACGCTTCTCAATTACGGTATCCATTATGACAATACCTGGATATATGACAAAATTCATCATGAGATCAATCAGTTCTGCACCTCACATTCTCTTCAAGAAGTTTATATTGATGTGTTCGATCAGATTGATGAAAAGATGAAAGAAGCTCTTCAGGGTGACTGTACCCGTTATGCTCCGGGTATTGAAATCATCAATGTCCGTGTTACAAAGCCTACCATCCCAGAAAGTATAAGACGTAACTTTGAGCAAATGGAAGAGGAGCGCACCAAGGTCTTAATTGCTATAGAGAGACAGAGAGTAGTGGAGAAAGAGGCAGAAACCAAGAAGAAGATGGCTATCAGTGAGGCTGAGAAGAATGCCAATGTCAGTAAGATTCTCATGGAACAAAAGTTGACGGAGAAGGACAGTGCTAGGAGGCAGCAGGAGATTGAAAACCAGATGTACACAGCTCGGGAAAGGAGCCTTGCAGATGCGGATTTCTACAAAGTTATGAAGGAAGCTGAAGCAAACAAGTTGAAGCTTACTCCTCAGTTTCTTGAGCTTAAATTCATCGATGCCATAGCTGATAACACTAAAATTTTCTTCGGAGACAAGGTTCCTAATATGGTTTTGGATCAGAGGTTGCTGGGGAACTTCTTACAGGTGCCGACAGAGGTGTCAAAAGAAGTGTCCGGGGAAGCTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

361

Amino Acids

41.11

Weight (kDa)

5.74

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 55 - 236 1e-27 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1051
AccB1I GGYRCC 1 cut(s) 1039
AciI CCGC 3 cut(s) 63, 66, 869
AclWI GGATC 3 cut(s) 12, 221, 1017
AcsI RAATTY 3 cut(s) 447, 941, 972
AcuI CTGAAG 2 cut(s) 530, 918
AfaI GTAC 3 cut(s) 322, 559, 839
AfiI CCNNNNNNNGG 2 cut(s) 59, 62
AgsI TTSAA 6 cut(s) 269, 278, 491, 581, 827, 913
AjnI CCWGG 3 cut(s) 22, 229, 431
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AloI GAACNNNNNNTCC 2 cut(s) 975, 1007
AluBI AGCT 9 cut(s) 16, 29, 542, 845, 896, 916, 937, 959, 1076
AluI AGCT 9 cut(s) 16, 29, 542, 845, 896, 916, 937, 959, 1076
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 4 cut(s) 13, 389, 679, 978
AlwI GGATC 3 cut(s) 12, 221, 1017
Ama87I CYCGRG 1 cut(s) 846
ApeKI GCWGC 1 cut(s) 814
ApoI RAATTY 3 cut(s) 447, 941, 972
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
Asp700I GAANNNNTTC 2 cut(s) 273, 543
AspLEI GCGC 2 cut(s) 206, 660
AsuC2I CCSGG 2 cut(s) 573, 1069
AsuHPI GGTGA 3 cut(s) 250, 313, 563
AvaI CYCGRG 1 cut(s) 846
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 1039
BanII GRGCYC 1 cut(s) 31
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 826
BccI CCATC 2 cut(s) 620, 721
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 3 cut(s) 24, 231, 433
BciVI GTATCC 1 cut(s) 425
BclI TGATCA 2 cut(s) 295, 339
BcnI CCSGG 2 cut(s) 573, 1069
BcoDI GTCTC 4 cut(s) 13, 389, 679, 978
BfaI CTAG 1 cut(s) 807
BfmI CTRYAG 2 cut(s) 287, 678
BfoI RGCGCY 1 cut(s) 207
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 3 cut(s) 64, 67, 815
BlsI GCNGC 3 cut(s) 65, 68, 816
Bme1390I CCNGG 5 cut(s) 24, 231, 433, 573, 1069
BmeT110I CYCGRG 1 cut(s) 846
BmiI GGNNCC 4 cut(s) 6, 857, 993, 1041
BmrFI CCNGG 5 cut(s) 24, 231, 433, 573, 1069
BmsI GCATC 2 cut(s) 856, 940
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 45
BpuEI CTTGAG 3 cut(s) 42, 227, 953
BpuMI CCSGG 2 cut(s) 573, 1069
Bsa29I ATCGAT 1 cut(s) 948
BsaBI GATNNNNATC 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 663, 1068
BsaXI ACNNNNNCTCC 2 cut(s) 975, 1005
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 62
Bse1I ACTGG 1 cut(s) 272
Bse8I GATNNNNATC 1 cut(s) 731
BseBI CCWGG 3 cut(s) 24, 231, 433
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 2 cut(s) 663, 1068
BseGI GGATG 1 cut(s) 612
BseJI GATNNNNATC 1 cut(s) 731
BseLI CCNNNNNNNGG 2 cut(s) 59, 62
BseMII CTCAG 4 cut(s) 8, 59, 735, 938
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 3 cut(s) 213, 668, 912
BseXI GCAGC 1 cut(s) 826
BseYI CCCAGC 1 cut(s) 1021
BsgI GTGCAG 2 cut(s) 143, 457
BshNI GGYRCC 1 cut(s) 1039
BshVI ATCGAT 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 846
BsiSI CCGG 2 cut(s) 572, 1068
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 59, 62
BsmAI GTCTC 4 cut(s) 13, 389, 679, 978
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 757
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 31
Bsp1407I TGTACA 1 cut(s) 837
Bsp143I GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
BspACI CCGC 3 cut(s) 63, 66, 869
BspCNI CTCAG 4 cut(s) 9, 58, 736, 937
BspDI ATCGAT 1 cut(s) 948
BspHI TCATGA 2 cut(s) 336, 454
BspLI GGNNCC 4 cut(s) 6, 857, 993, 1041
BspMAI CTGCAG 1 cut(s) 291
BspPI GGATC 3 cut(s) 12, 221, 1017
BspQI GCTCTTC 2 cut(s) 213, 549
BspT107I GGYRCC 1 cut(s) 1039
BspTNI GGTCTC 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 837
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 663, 1068
BssMI GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 3 cut(s) 24, 231, 433
Bst4CI ACNGT 4 cut(s) 127, 413, 557, 803
Bst6I CTCTTC 4 cut(s) 213, 492, 549, 645
BstAUI TGTACA 1 cut(s) 837
BstDEI CTNAG 4 cut(s) 17, 45, 744, 924
BstEII GGTNACC 1 cut(s) 256
BstF5I GGATG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 206, 660
BstKTI GATC 7 cut(s) 7, 229, 298, 342, 462, 517, 1012
BstMAI GTCTC 4 cut(s) 13, 389, 679, 978
BstMBI GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 107, 116
BstNI CCWGG 3 cut(s) 24, 231, 433
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 5 cut(s) 22, 229, 431, 571, 1067
BstSFI CTRYAG 2 cut(s) 287, 678
BstV1I GCAGC 1 cut(s) 826
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 4, 226
BstYI RGATCY 2 cut(s) 4, 226
Bsu15I ATCGAT 1 cut(s) 948
BsuI GTATCC 1 cut(s) 425
BsuTUI ATCGAT 1 cut(s) 948
BtsCI GGATG 1 cut(s) 612
BtsIMutI CAGTG 2 cut(s) 742, 808
CciI TCATGA 2 cut(s) 336, 454
CfoI GCGC 2 cut(s) 206, 660
ClaI ATCGAT 1 cut(s) 948
CseI GACGC 1 cut(s) 406
Csp6I GTAC 3 cut(s) 321, 558, 838
CviAII CATG 5 cut(s) 179, 315, 337, 455, 775
CviQI GTAC 3 cut(s) 321, 558, 838
DdeI CTNAG 4 cut(s) 17, 45, 744, 924
DpnI GATC 7 cut(s) 6, 228, 297, 341, 461, 516, 1011
DpnII GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
DrdI GACNNNNNNGTC 1 cut(s) 1051
DseDI GACNNNNNNGTC 1 cut(s) 1051
Eam1104I CTCTTC 4 cut(s) 213, 492, 549, 645
EarI CTCTTC 4 cut(s) 213, 492, 549, 645
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 530, 918
Eco88I CYCGRG 1 cut(s) 846
Eco91I GGTNACC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 29
EcoO65I GGTNACC 1 cut(s) 256
EcoRII CCWGG 3 cut(s) 22, 229, 431
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 663
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 5 cut(s) 182, 318, 340, 458, 778
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 1 cut(s) 152
FatI CATG 5 cut(s) 178, 314, 336, 454, 774
FbaI TGATCA 2 cut(s) 295, 339
Fnu4HI GCNGC 3 cut(s) 64, 67, 815
FokI GGATG 1 cut(s) 599
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 64, 67, 815
FspBI CTAG 1 cut(s) 807
GlaI GCGC 2 cut(s) 205, 659
GluI GCNGC 3 cut(s) 64, 67, 815
GsaI CCCAGC 1 cut(s) 1025
HaeII RGCGCY 1 cut(s) 207
HapII CCGG 2 cut(s) 572, 1068
HgaI GACGC 1 cut(s) 406
HhaI GCGC 2 cut(s) 206, 660
Hin1II CATG 5 cut(s) 182, 318, 340, 458, 778
Hin6I GCGC 2 cut(s) 204, 658
HinP1I GCGC 2 cut(s) 204, 658
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 2 cut(s) 71, 769
HpaII CCGG 2 cut(s) 572, 1068
HphI GGTGA 3 cut(s) 250, 313, 563
Hpy166II GTNNAC 3 cut(s) 367, 789, 840
Hpy188I TCNGA 3 cut(s) 519, 983, 1014
Hpy188III TCNNGA 5 cut(s) 337, 455, 491, 848, 932
Hpy8I GTNNAC 3 cut(s) 367, 789, 840
HpyAV CCTTC 3 cut(s) 167, 790, 883
HpyCH4III ACNGT 4 cut(s) 127, 413, 557, 803
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 4 cut(s) 160, 289, 474, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 116
HpyF3I CTNAG 4 cut(s) 17, 45, 744, 924
HpySE526I ACGT 1 cut(s) 631
Hsp92II CATG 5 cut(s) 182, 318, 340, 458, 778
HspAI GCGC 2 cut(s) 204, 658
Ksp22I TGATCA 2 cut(s) 295, 339
Kzo9I GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
LguI GCTCTTC 2 cut(s) 213, 549
LmnI GCTCC 5 cut(s) 26, 34, 574, 655, 855
Lsp1109I GCAGC 1 cut(s) 826
LweI GCATC 2 cut(s) 856, 940
MaeI CTAG 1 cut(s) 807
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 6 cut(s) 121, 256, 280, 551, 598, 632
MalI GATC 7 cut(s) 6, 228, 297, 341, 461, 516, 1011
MboI GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
MflI RGATCY 2 cut(s) 4, 226
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 7 cut(s) 219, 343, 406, 447, 672, 941, 972
MmeI TCCRAC 1 cut(s) 158
MroXI GAANNNNTTC 2 cut(s) 273, 543
MseI TTAA 2 cut(s) 671, 939
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 572, 1068
MspR9I CCNGG 5 cut(s) 24, 231, 433, 573, 1069
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 3 cut(s) 24, 231, 433
MwoI GCNNNNNNNGC 2 cut(s) 107, 116
NciI CCSGG 2 cut(s) 573, 1069
NdeII GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
NlaIII CATG 5 cut(s) 182, 318, 340, 458, 778
NlaIV GGNNCC 4 cut(s) 6, 857, 993, 1041
NmuCI GTSAC 4 cut(s) 121, 256, 280, 551
PagI TCATGA 2 cut(s) 336, 454
PciSI GCTCTTC 2 cut(s) 213, 549
PctI GAATGC 1 cut(s) 757
PdmI GAANNNNTTC 2 cut(s) 273, 543
PfeI GAWTC 2 cut(s) 71, 769
PkrI GCNGC 3 cut(s) 65, 68, 816
Psp124BI GAGCTC 1 cut(s) 31
Psp6I CCWGG 3 cut(s) 22, 229, 431
PspEI GGTNACC 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 1021
PspGI CCWGG 3 cut(s) 22, 229, 431
PspN4I GGNNCC 4 cut(s) 6, 857, 993, 1041
PstI CTGCAG 1 cut(s) 291
PsuI RGATCY 2 cut(s) 4, 226
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 3 cut(s) 322, 559, 839
RsaNI GTAC 3 cut(s) 321, 558, 838
SacI GAGCTC 1 cut(s) 31
SapI GCTCTTC 2 cut(s) 213, 549
SaqAI TTAA 2 cut(s) 671, 939
SatI GCNGC 3 cut(s) 64, 67, 815
Sau3AI GATC 7 cut(s) 4, 226, 295, 339, 459, 514, 1009
ScrFI CCNGG 5 cut(s) 24, 231, 433, 573, 1069
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 2 cut(s) 856, 940
SfcI CTRYAG 2 cut(s) 287, 678
SmlI CTYRAG 3 cut(s) 57, 242, 932
SmoI CTYRAG 3 cut(s) 57, 242, 932
Sse9I AATT 7 cut(s) 219, 343, 406, 447, 672, 941, 972
SsiI CCGC 3 cut(s) 63, 66, 869
SspMI CTAG 1 cut(s) 807
SstI GAGCTC 1 cut(s) 31
StyD4I CCNGG 5 cut(s) 22, 229, 431, 571, 1067
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 4 cut(s) 127, 413, 557, 803
TaiI ACGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 513, 948
TasI AATT 7 cut(s) 219, 343, 406, 447, 672, 941, 972
TatI WGTACW 1 cut(s) 837
TauI GCSGC 2 cut(s) 66, 69
TfiI GAWTC 2 cut(s) 71, 769
Tru1I TTAA 2 cut(s) 671, 939
Tru9I TTAA 2 cut(s) 671, 939
TscAI CASTG 2 cut(s) 742, 808
TseFI GTSAC 4 cut(s) 121, 256, 280, 551
TseI GCWGC 1 cut(s) 814
Tsp45I GTSAC 4 cut(s) 121, 256, 280, 551
TspDTI ATGAA 6 cut(s) 227, 440, 540, 548, 902, 934
TspGWI ACGGA 2 cut(s) 584, 806
TspRI CASTG 2 cut(s) 742, 808
XapI RAATTY 3 cut(s) 447, 941, 972
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmnI GAANNNNTTC 2 cut(s) 273, 543
XspI CTAG 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.