MD13G1137000.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006540
Physical Location & Seq
Forward (+)
10537566 .. 10539193
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1137000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGGAAGAGCTGCTGCAGCTCATTCATCATCTTCTTCAATAGATAGCAGCAACTATCCTGATCATCATCGTCCTAGCACTACTGCCTCTTCATCTTCTTCCTCTCTTTCTCGGAAAATCAATGGAAATTCCAGAGCAGATTTGAGCACTGATCTGAGACTTGGCCTGAGCATCTCTCCCTCTCATCACAGTGACTTGCCTTCCACTTCAACCCCAAGGGAGCAAGCATTGATAAACTGGCCACCAATCAAATCTATCTTGAGGAGCACACTTGCAGGGAGAGCAGCAGACAACAGGCGCCATCCCTCCCTATTTGTGAAAGTCTATTTGGAGGGCATTCCAATTGGTAGAAAATTGAATTTGTTTGACCATGATGGTTATTCTGCTTTGATTTCAACGCTTTGCCTCATGTTCAAGACCACCATTCTATGTCCTGAGGAAAATCATGTTCATTCAGACAAATATCATGTACTGACTTATCAAGATCAAGAAGGGGACTGGATGCTGGTTGGAGATGTGCCTTGGGAGATATTCTTGACCACCGTGAAAAGATTGAAGATCACTAGAGCAGACAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

194

Amino Acids

21.42

Weight (kDa)

8.83

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 68 - 189 1.9e-29 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 297
AcoI YGGCCR 1 cut(s) 239
AcsI RAATTY 2 cut(s) 127, 358
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 1 cut(s) 471
AgsI TTSAA 6 cut(s) 39, 210, 358, 396, 415, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 2 cut(s) 11, 20
AluI AGCT 2 cut(s) 11, 20
Alw21I GWGCWC 2 cut(s) 149, 269
Alw26I GTCTC 1 cut(s) 151
AoxI GGCC 2 cut(s) 163, 239
ApeKI GCWGC 5 cut(s) 11, 14, 17, 48, 284
ApoI RAATTY 2 cut(s) 127, 358
AspLEI GCGC 1 cut(s) 300
AxyI CCTNAGG 1 cut(s) 435
BalI TGGCCA 1 cut(s) 241
BanI GGYRCC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 149, 269
BbvI GCAGC 3 cut(s) 29, 60, 296
BccI CCATC 2 cut(s) 309, 368
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 75, 564
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 5 cut(s) 12, 15, 18, 49, 285
BlsI GCNGC 5 cut(s) 13, 16, 19, 50, 286
BmiI GGNNCC 1 cut(s) 299
BmsI GCATC 2 cut(s) 180, 492
BplI GAGNNNNNCTC 2 cut(s) 160, 192
Bpu10I CCTNAGC 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 215, 521
Bse1I ACTGG 2 cut(s) 242, 503
Bse21I CCTNAGG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 215, 521
BseGI GGATG 2 cut(s) 301, 507
BseJI GATNNNNATC 1 cut(s) 66
BseMII CTCAG 3 cut(s) 146, 158, 426
BseNI ACTGG 2 cut(s) 242, 503
BseRI GAGGAG 1 cut(s) 277
BseXI GCAGC 3 cut(s) 29, 60, 296
BshFI GGCC 2 cut(s) 165, 241
BshNI GGYRCC 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 149, 269
BslFI GGGAC 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 165, 241
Bsp1286I GDGCHC 2 cut(s) 149, 269
Bsp143I GATC 4 cut(s) 61, 151, 484, 558
BspANI GGCC 2 cut(s) 165, 241
BspCNI CTCAG 3 cut(s) 147, 159, 427
BspLI GGNNCC 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 297
BsrI ACTGG 2 cut(s) 242, 503
BssECI CCNNGG 2 cut(s) 215, 521
BssMI GATC 4 cut(s) 61, 151, 484, 558
BssNI GRCGYC 1 cut(s) 298
BssT1I CCWWGG 2 cut(s) 215, 521
Bst4CI ACNGT 2 cut(s) 191, 544
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 298
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 3 cut(s) 155, 167, 435
BstF5I GGATG 2 cut(s) 301, 507
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 4 cut(s) 64, 154, 487, 561
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 4 cut(s) 61, 151, 484, 558
BstMWI GCNNNNNNNGC 2 cut(s) 17, 281
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 3 cut(s) 29, 60, 296
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 2 cut(s) 165, 241
BtsCI GGATG 2 cut(s) 301, 507
BtsIMutI CAGTG 2 cut(s) 147, 196
Cac8I GCNNGC 1 cut(s) 225
CfoI GCGC 1 cut(s) 300
Csp6I GTAC 1 cut(s) 470
CviAII CATG 4 cut(s) 371, 409, 446, 467
CviJI RGCY 4 cut(s) 11, 20, 165, 241
CviKI_1 RGCY 4 cut(s) 11, 20, 165, 241
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 3 cut(s) 155, 167, 435
DinI GGCGCC 1 cut(s) 299
DpnI GATC 4 cut(s) 63, 153, 486, 560
DpnII GATC 4 cut(s) 61, 151, 484, 558
EaeI YGGCCR 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 215, 521
Eco81I CCTNAGG 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 215, 521
EgeI GGCGCC 1 cut(s) 299
EheI GGCGCC 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 215, 521
FaeI CATG 4 cut(s) 374, 412, 449, 470
FaiI YATR 5 cut(s) 372, 410, 430, 447, 468
FaqI GGGAC 1 cut(s) 509
FatI CATG 4 cut(s) 370, 408, 445, 466
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 12, 15, 18, 49, 285
FokI GGATG 2 cut(s) 288, 514
Fsp4HI GCNGC 5 cut(s) 12, 15, 18, 49, 285
FspBI CTAG 2 cut(s) 75, 564
GlaI GCGC 1 cut(s) 299
GluI GCNGC 5 cut(s) 12, 15, 18, 49, 285
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 2 cut(s) 165, 241
HhaI GCGC 1 cut(s) 300
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 4 cut(s) 374, 412, 449, 470
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
Hpy188I TCNGA 3 cut(s) 114, 156, 457
Hpy188III TCNNGA 8 cut(s) 59, 132, 259, 415, 434, 482, 488, 535
HpyAV CCTTC 2 cut(s) 210, 485
HpyCH4III ACNGT 2 cut(s) 191, 544
HpyCH4V TGCA 2 cut(s) 17, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 281
HpyF3I CTNAG 3 cut(s) 155, 167, 435
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 4 cut(s) 374, 412, 449, 470
HspAI GCGC 1 cut(s) 298
KasI GGCGCC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 61, 151, 484, 558
LmnI GCTCC 2 cut(s) 220, 264
LpnPI CCDG 9 cut(s) 72, 145, 179, 223, 261, 280, 447, 484, 491
Lsp1109I GCAGC 3 cut(s) 29, 60, 296
LweI GCATC 2 cut(s) 180, 492
MaeI CTAG 2 cut(s) 75, 564
MaeIII GTNAC 1 cut(s) 191
MalI GATC 4 cut(s) 63, 153, 486, 560
MboI GATC 4 cut(s) 61, 151, 484, 558
MboII GAAGA 7 cut(s) 18, 24, 27, 81, 87, 90, 568
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 149, 269
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 4 cut(s) 127, 342, 353, 358
MluNI TGGCCA 1 cut(s) 241
Mly113I GGCGCC 1 cut(s) 298
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 8 cut(s) 97, 112, 190, 255, 316, 325, 416, 430
Mox20I TGGCCA 1 cut(s) 241
MscI TGGCCA 1 cut(s) 241
MslI CAYNNNNRTG 1 cut(s) 189
Msp20I TGGCCA 1 cut(s) 241
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 17, 281
NarI GGCGCC 1 cut(s) 298
NdeII GATC 4 cut(s) 61, 151, 484, 558
NlaIII CATG 4 cut(s) 374, 412, 449, 470
NlaIV GGNNCC 1 cut(s) 299
NmuCI GTSAC 1 cut(s) 191
PctI GAATGC 1 cut(s) 336
PkrI GCNGC 5 cut(s) 13, 16, 19, 50, 286
PluTI GGCGCC 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 299
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 189
SatI GCNGC 5 cut(s) 12, 15, 18, 49, 285
Sau3AI GATC 4 cut(s) 61, 151, 484, 558
SduI GDGCHC 2 cut(s) 149, 269
SetI ASST 2 cut(s) 13, 22
SfaNI GCATC 2 cut(s) 180, 492
SfcI CTRYAG 1 cut(s) 15
SfoI GGCGCC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
Sse9I AATT 4 cut(s) 127, 342, 353, 358
SspDI GGCGCC 1 cut(s) 297
SspMI CTAG 2 cut(s) 75, 564
StyI CCWWGG 2 cut(s) 215, 521
TaaI ACNGT 2 cut(s) 191, 544
TasI AATT 4 cut(s) 127, 342, 353, 358
TatI WGTACW 1 cut(s) 469
TscAI CASTG 2 cut(s) 154, 196
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 5 cut(s) 11, 14, 17, 48, 284
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 15, 81, 440
TspRI CASTG 2 cut(s) 154, 196
XapI RAATTY 2 cut(s) 127, 358
XspI CTAG 2 cut(s) 75, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.