Rh4DG281200

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006540
Physical Location & Seq
Reverse (-)
50333677 .. 50336671
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG281200.1

Sequence Viewer

Length: 507 bp
ATGGGATTACGAGCTGCTGCACATTCATCATCTTCTTCCATAGACAGCAGCAACCATCCTGACCTTGATGGTCTTCTTCCTAAGCCTTCTGGATCGTCTTCTTCTTCGTCTCTCTCCCACAAAATCAATGGTGGTTCTAATTCTCGTAACAGTCGCAGAAGCAATCATACCAGAACAGATTTGAGCACTGATCTCAGACTTGGCCTGAGCATATCACCGTCTCATCACTCTGACTTGTCTTCCACTACTTCAAGCCCAAGGGAAGAAGCATTGGTAAGCTGGCCGCCGATCAAATCGATCTTGAGGAGCACACTTTCAGGCAAATCAGAGAATTACCATCATCAGAATTCTTCTTTATTTGTGAAGGTCTACATGGAGGGAATCCCAATTGGTAGAAAATTGAATCTGTTTGCTCACGATGGTTATGATGCTTTGATCACAACTCTGAGCCTCATGTTCAAGACCACCATTCTATGTAAGTTGACGCAAACACAAATTGTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

168

Amino Acids

18.17

Weight (kDa)

9.61

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 78 - 161 1.5e-15 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AciI CCGC 1 cut(s) 284
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 346
AgsI TTSAA 3 cut(s) 252, 403, 460
AluBI AGCT 2 cut(s) 14, 279
AluI AGCT 2 cut(s) 14, 279
Alw21I GWGCWC 2 cut(s) 188, 311
Alw26I GTCTC 2 cut(s) 114, 225
AlwI GGATC 1 cut(s) 100
AoxI GGCC 2 cut(s) 202, 281
ApeKI GCWGC 3 cut(s) 14, 17, 48
ApoI RAATTY 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 151, 183
BbsI GAAGAC 3 cut(s) 65, 90, 231
Bbv12I GWGCWC 2 cut(s) 188, 311
BbvI GCAGC 2 cut(s) 4, 60
BccI CCATC 4 cut(s) 62, 63, 345, 413
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 114, 225
BisI GCNGC 4 cut(s) 15, 18, 49, 284
BlsI GCNGC 4 cut(s) 16, 19, 50, 285
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 3 cut(s) 65, 90, 231
Bpu10I CCTNAGC 2 cut(s) 81, 206
BpuEI CTTGAG 1 cut(s) 322
Bsa29I ATCGAT 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 1 cut(s) 55
BseMII CTCAG 3 cut(s) 197, 208, 437
BseRI GAGGAG 1 cut(s) 319
BseXI GCAGC 2 cut(s) 4, 60
BshFI GGCC 2 cut(s) 204, 283
BshVI ATCGAT 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 188, 311
BsmAI GTCTC 2 cut(s) 114, 225
BsmBI CGTCTC 2 cut(s) 114, 225
BsnI GGCC 2 cut(s) 204, 283
Bsp1286I GDGCHC 2 cut(s) 188, 311
Bsp143I GATC 5 cut(s) 92, 190, 288, 297, 435
BspACI CCGC 1 cut(s) 284
BspANI GGCC 2 cut(s) 204, 283
BspCNI CTCAG 3 cut(s) 198, 207, 438
BspDI ATCGAT 1 cut(s) 296
BspPI GGATC 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 5 cut(s) 92, 190, 288, 297, 435
BssT1I CCWWGG 1 cut(s) 257
Bst4CI ACNGT 2 cut(s) 152, 219
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 4 cut(s) 81, 194, 206, 446
BstF5I GGATG 1 cut(s) 55
BstKTI GATC 5 cut(s) 95, 193, 291, 300, 438
BstMAI GTCTC 2 cut(s) 114, 225
BstMBI GATC 5 cut(s) 92, 190, 288, 297, 435
BstV1I GCAGC 2 cut(s) 4, 60
BstV2I GAAGAC 3 cut(s) 65, 90, 231
Bsu15I ATCGAT 1 cut(s) 296
BsuRI GGCC 2 cut(s) 204, 283
BsuTUI ATCGAT 1 cut(s) 296
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 281
ClaI ATCGAT 1 cut(s) 296
CseI GACGC 1 cut(s) 493
CviAII CATG 2 cut(s) 373, 454
CviJI RGCY 7 cut(s) 14, 85, 204, 255, 279, 283, 450
CviKI_1 RGCY 7 cut(s) 14, 85, 204, 255, 279, 283, 450
DdeI CTNAG 4 cut(s) 81, 194, 206, 446
DpnI GATC 5 cut(s) 94, 192, 290, 299, 437
DpnII GATC 5 cut(s) 92, 190, 288, 297, 435
EaeI YGGCCR 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
Esp3I CGTCTC 2 cut(s) 114, 225
FaeI CATG 2 cut(s) 376, 457
FaiI YATR 9 cut(s) 41, 168, 212, 374, 426, 455, 475, 503, 505
FatI CATG 2 cut(s) 372, 453
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 4 cut(s) 15, 18, 49, 284
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 15, 18, 49, 284
GluI GCNGC 4 cut(s) 15, 18, 49, 284
HaeIII GGCC 2 cut(s) 204, 283
HgaI GACGC 1 cut(s) 493
Hin1II CATG 2 cut(s) 376, 457
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HinfI GANTC 2 cut(s) 381, 403
HphI GGTGA 1 cut(s) 207
Hpy166II GTNNAC 2 cut(s) 370, 483
Hpy188I TCNGA 5 cut(s) 197, 232, 328, 345, 447
Hpy188III TCNNGA 5 cut(s) 59, 90, 301, 416, 460
Hpy8I GTNNAC 2 cut(s) 370, 483
HpyAV CCTTC 2 cut(s) 96, 358
HpyCH4III ACNGT 2 cut(s) 152, 219
HpyCH4V TGCA 1 cut(s) 20
HpyF3I CTNAG 4 cut(s) 81, 194, 206, 446
Hsp92II CATG 2 cut(s) 376, 457
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 5 cut(s) 92, 190, 288, 297, 435
LmnI GCTCC 1 cut(s) 306
LpnPI CCDG 6 cut(s) 72, 75, 184, 218, 265, 303
Lsp1109I GCAGC 2 cut(s) 4, 60
LweI GCATC 1 cut(s) 418
MaeIII GTNAC 1 cut(s) 146
MalI GATC 5 cut(s) 94, 192, 290, 299, 437
MboI GATC 5 cut(s) 92, 190, 288, 297, 435
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 188, 311
MluCI AATT 6 cut(s) 139, 331, 346, 387, 398, 495
MnlI CCTC 3 cut(s) 297, 370, 461
MunI CAATTG 1 cut(s) 387
NdeII GATC 5 cut(s) 92, 190, 288, 297, 435
NlaIII CATG 2 cut(s) 376, 457
PfeI GAWTC 2 cut(s) 381, 403
PkrI GCNGC 4 cut(s) 16, 19, 50, 285
SatI GCNGC 4 cut(s) 15, 18, 49, 284
Sau3AI GATC 5 cut(s) 92, 190, 288, 297, 435
SduI GDGCHC 2 cut(s) 188, 311
SetI ASST 4 cut(s) 16, 66, 281, 369
SfaNI GCATC 1 cut(s) 418
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 6 cut(s) 139, 331, 346, 387, 398, 495
SsiI CCGC 1 cut(s) 284
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 2 cut(s) 152, 219
TaqI TCGA 1 cut(s) 296
TasI AATT 6 cut(s) 139, 331, 346, 387, 398, 495
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 2 cut(s) 381, 403
TscAI CASTG 1 cut(s) 193
TseI GCWGC 3 cut(s) 14, 17, 48
TspDTI ATGAA 1 cut(s) 15
TspRI CASTG 1 cut(s) 193
XapI RAATTY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.