Rw4G024210

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006540
Physical Location & Seq
Reverse (-)
50063411 .. 50064467
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024210.1

Sequence Viewer

Length: 594 bp
ATGGGATTACGAGCTGCTGCACATTCATCATCTTCTTCCATAGACAGCAGCAACCATCCTGACCTGATGGTCTTCTTCCTAAGCCTTCTGGATCGTCTTCTTCTTCGTCTCTCTCCACAAAATCAATGCAATCATACCAGAACAGATTTGAGCACTGATCTCAGACTTGGCCTGAGCATATCACCGTCTCATCACTCTGACTTGTCTTCCACTACTTCAAGCCCAAGGGAAGAAGCATTGGTAAGCTGGCCGCCGATCAAATCGATCTTGAGGAGCACACTTTCAGGCAAATCAGAGAATTACCATCATCAGAATTCTTCTTTATTTGTGAAGGTCTACATGGAGGGAATCCCAATTGGTAGAAAATTGAATCTGTTTGCTCACGATGGTTATGATGCTTTGATCACAACTCTGAGCCTCATGTTCAAGACCACCATTCTATGTCCTGATAATAATCATGTTCATTCAGAGAAATATTATGTTTTAACTTATGAAGATCAAGAAGGGGATTGGATGCTGGTTGGAGATGTGCCTTGGGAGATATTCTTGACTACTGTGAAAAGACTGAAGATTACTAGAGCAGACAGATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

197

Amino Acids

22.26

Weight (kDa)

6.54

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 72 - 193 6.9e-34 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 251
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 587
AgsI TTSAA 3 cut(s) 219, 370, 427
AluBI AGCT 2 cut(s) 14, 246
AluI AGCT 2 cut(s) 14, 246
Alw21I GWGCWC 2 cut(s) 155, 278
Alw26I GTCTC 2 cut(s) 113, 192
AlwI GGATC 1 cut(s) 99
AoxI GGCC 2 cut(s) 169, 248
ApeKI GCWGC 3 cut(s) 14, 17, 48
ApoI RAATTY 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 174
BbsI GAAGAC 3 cut(s) 64, 89, 198
Bbv12I GWGCWC 2 cut(s) 155, 278
BbvI GCAGC 2 cut(s) 4, 60
BccI CCATC 4 cut(s) 61, 63, 312, 380
BclI TGATCA 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 113, 192
BfaI CTAG 1 cut(s) 576
BisI GCNGC 4 cut(s) 15, 18, 49, 251
BlsI GCNGC 4 cut(s) 16, 19, 50, 252
BmsI GCATC 2 cut(s) 385, 504
BpiI GAAGAC 3 cut(s) 64, 89, 198
Bpu10I CCTNAGC 2 cut(s) 80, 173
BpuEI CTTGAG 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 224, 533
Bse8I GATNNNNATC 1 cut(s) 453
BseCI ATCGAT 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 224, 533
BseGI GGATG 2 cut(s) 55, 519
BseJI GATNNNNATC 1 cut(s) 453
BseMII CTCAG 3 cut(s) 164, 175, 404
BseRI GAGGAG 1 cut(s) 286
BseXI GCAGC 2 cut(s) 4, 60
BshFI GGCC 2 cut(s) 171, 250
BshVI ATCGAT 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 155, 278
BsmAI GTCTC 2 cut(s) 113, 192
BsmBI CGTCTC 2 cut(s) 113, 192
BsnI GGCC 2 cut(s) 171, 250
Bsp1286I GDGCHC 2 cut(s) 155, 278
Bsp143I GATC 6 cut(s) 91, 157, 255, 264, 402, 496
BspACI CCGC 1 cut(s) 251
BspANI GGCC 2 cut(s) 171, 250
BspCNI CTCAG 3 cut(s) 165, 174, 405
BspDI ATCGAT 1 cut(s) 263
BspPI GGATC 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 224, 533
BssMI GATC 6 cut(s) 91, 157, 255, 264, 402, 496
BssT1I CCWWGG 2 cut(s) 224, 533
Bst4CI ACNGT 2 cut(s) 186, 556
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 4 cut(s) 80, 161, 173, 413
BstF5I GGATG 2 cut(s) 55, 519
BstKTI GATC 6 cut(s) 94, 160, 258, 267, 405, 499
BstMAI GTCTC 2 cut(s) 113, 192
BstMBI GATC 6 cut(s) 91, 157, 255, 264, 402, 496
BstV1I GCAGC 2 cut(s) 4, 60
BstV2I GAAGAC 3 cut(s) 64, 89, 198
Bsu15I ATCGAT 1 cut(s) 263
BsuRI GGCC 2 cut(s) 171, 250
BsuTUI ATCGAT 1 cut(s) 263
BtsCI GGATG 2 cut(s) 55, 519
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 248
ClaI ATCGAT 1 cut(s) 263
CviAII CATG 3 cut(s) 340, 421, 458
CviJI RGCY 7 cut(s) 14, 84, 171, 222, 246, 250, 417
CviKI_1 RGCY 7 cut(s) 14, 84, 171, 222, 246, 250, 417
DdeI CTNAG 4 cut(s) 80, 161, 173, 413
DpnI GATC 6 cut(s) 93, 159, 257, 266, 404, 498
DpnII GATC 6 cut(s) 91, 157, 255, 264, 402, 496
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 248
Eco130I CCWWGG 2 cut(s) 224, 533
Eco57I CTGAAG 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 224, 533
ErhI CCWWGG 2 cut(s) 224, 533
Esp3I CGTCTC 2 cut(s) 113, 192
FaeI CATG 3 cut(s) 343, 424, 461
FatI CATG 3 cut(s) 339, 420, 457
FbaI TGATCA 1 cut(s) 402
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 15, 18, 49, 251
FokI GGATG 2 cut(s) 42, 526
Fsp4HI GCNGC 4 cut(s) 15, 18, 49, 251
FspBI CTAG 1 cut(s) 576
GluI GCNGC 4 cut(s) 15, 18, 49, 251
HaeIII GGCC 2 cut(s) 171, 250
Hin1II CATG 3 cut(s) 343, 424, 461
HinfI GANTC 2 cut(s) 348, 370
HphI GGTGA 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 6 cut(s) 164, 199, 295, 312, 414, 469
Hpy188III TCNNGA 8 cut(s) 59, 89, 268, 383, 427, 446, 500, 547
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 3 cut(s) 95, 325, 497
HpyCH4III ACNGT 2 cut(s) 186, 556
HpyCH4V TGCA 2 cut(s) 20, 129
HpyF3I CTNAG 4 cut(s) 80, 161, 173, 413
Hsp92II CATG 3 cut(s) 343, 424, 461
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 6 cut(s) 91, 157, 255, 264, 402, 496
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 9 cut(s) 72, 74, 77, 151, 185, 232, 270, 459, 503
Lsp1109I GCAGC 2 cut(s) 4, 60
LweI GCATC 2 cut(s) 385, 504
MaeI CTAG 1 cut(s) 576
MalI GATC 6 cut(s) 93, 159, 257, 266, 404, 498
MboI GATC 6 cut(s) 91, 157, 255, 264, 402, 496
MfeI CAATTG 1 cut(s) 354
MhlI GDGCHC 2 cut(s) 155, 278
MluCI AATT 4 cut(s) 298, 313, 354, 365
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 3 cut(s) 264, 337, 428
MseI TTAA 1 cut(s) 485
MunI CAATTG 1 cut(s) 354
NdeII GATC 6 cut(s) 91, 157, 255, 264, 402, 496
NlaIII CATG 3 cut(s) 343, 424, 461
PfeI GAWTC 2 cut(s) 348, 370
PkrI GCNGC 4 cut(s) 16, 19, 50, 252
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 4 cut(s) 15, 18, 49, 251
Sau3AI GATC 6 cut(s) 91, 157, 255, 264, 402, 496
SduI GDGCHC 2 cut(s) 155, 278
SetI ASST 4 cut(s) 16, 66, 248, 336
SfaNI GCATC 2 cut(s) 385, 504
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 4 cut(s) 298, 313, 354, 365
SsiI CCGC 1 cut(s) 251
SspI AATATT 1 cut(s) 476
SspMI CTAG 1 cut(s) 576
StyI CCWWGG 2 cut(s) 224, 533
TaaI ACNGT 2 cut(s) 186, 556
TaqI TCGA 1 cut(s) 263
TasI AATT 4 cut(s) 298, 313, 354, 365
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 2 cut(s) 348, 370
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 1 cut(s) 160
TseI GCWGC 3 cut(s) 14, 17, 48
TspDTI ATGAA 3 cut(s) 15, 452, 507
TspRI CASTG 1 cut(s) 160
XapI RAATTY 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.