Rh4AG285900

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006540
Physical Location & Seq
Reverse (-)
60029268 .. 60030731
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG285900.1

Sequence Viewer

Length: 486 bp
ATGTCTGCATCATCCAGAAACACTCGTACTTGGCCAAGTGTGTGCACTGATCTCACACTTGGCCGGAGCATCTCACCTTCTCTCCTCAATACTTCAAAGCCAAGGGAAGAAGCATTGAGGAGCACACTTCCAGGCAAAGCAGAGAATTACCATGATCAAAATTCTTCTTTATCTGTCAAGGTCTACATGGAGGGAATCCCAATTGGCAGAAAAGTGAATCTGTTTGCTCATGATGGTTATGATGCTTTGATCACATCTCTGAGCCTCATGTTCAAGATCACCATTCTATGTCCTGATCAAGTTTATTCAGAGAAAAATCATGTGTTAACTTACGAAGATCAAGAAGGGGATTGGATGCTGGTTGGAGATGTGCCTTGGGAGATATTCTTGACTAGTGTGAAAAAACTGATGATCACTAGAGCGGACAGATGTGGAAGCAGCTGGAATACTCCGAGAGAGCAAGTGACAACCAAGGACCGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

161

Amino Acids

18.24

Weight (kDa)

7.72

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 46 - 140 1.3e-26 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 183
AciI CCGC 1 cut(s) 422
AcoI YGGCCR 2 cut(s) 32, 61
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 1 cut(s) 28
AgsI TTSAA 2 cut(s) 96, 274
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 1 cut(s) 441
AluI AGCT 1 cut(s) 441
Alw21I GWGCWC 2 cut(s) 47, 125
Alw44I GTGCAC 1 cut(s) 43
AoxI GGCC 2 cut(s) 32, 61
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 475
AsuHPI GGTGA 2 cut(s) 66, 271
AvaII GGWCC 1 cut(s) 475
BaeGI GKGCMC 1 cut(s) 47
BalI TGGCCA 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 47, 125
BbvI GCAGC 1 cut(s) 450
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 132
BclI TGATCA 4 cut(s) 154, 249, 295, 411
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 393, 417
BisI GCNGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 440
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 4 cut(s) 17, 78, 232, 345
BsaJI CCNNGG 3 cut(s) 101, 374, 471
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 3 cut(s) 101, 374, 471
BseGI GGATG 2 cut(s) 11, 360
BseMII CTCAG 1 cut(s) 251
BseRI GAGGAG 2 cut(s) 74, 133
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 1 cut(s) 450
BshFI GGCC 2 cut(s) 34, 63
BsiHKAI GWGCWC 2 cut(s) 47, 125
BsiSI CCGG 1 cut(s) 64
BsnI GGCC 2 cut(s) 34, 63
Bsp1286I GDGCHC 2 cut(s) 47, 125
Bsp143I GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
BspACI CCGC 1 cut(s) 422
BspANI GGCC 2 cut(s) 34, 63
BspCNI CTCAG 1 cut(s) 252
BspHI TCATGA 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 422
BssECI CCNNGG 3 cut(s) 101, 374, 471
BssMI GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
BssT1I CCWWGG 3 cut(s) 101, 374, 471
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 479
BstDEI CTNAG 1 cut(s) 260
BstF5I GGATG 2 cut(s) 11, 360
BstKTI GATC 7 cut(s) 52, 157, 252, 279, 298, 340, 414
BstMBI GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 34, 63
BtsCI GGATG 2 cut(s) 11, 360
BtsIMutI CAGTG 1 cut(s) 45
CciI TCATGA 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 475
Csp6I GTAC 1 cut(s) 27
CviAII CATG 5 cut(s) 152, 187, 230, 268, 320
CviJI RGCY 5 cut(s) 34, 63, 100, 264, 441
CviKI_1 RGCY 5 cut(s) 34, 63, 100, 264, 441
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 1 cut(s) 260
DpnI GATC 7 cut(s) 51, 156, 251, 278, 297, 339, 413
DpnII GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
EaeI YGGCCR 2 cut(s) 32, 61
Eco130I CCWWGG 3 cut(s) 101, 374, 471
Eco47I GGWCC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 3 cut(s) 101, 374, 471
ErhI CCWWGG 3 cut(s) 101, 374, 471
FaeI CATG 5 cut(s) 155, 190, 233, 271, 323
FaiI YATR 7 cut(s) 153, 188, 231, 240, 269, 289, 321
FatI CATG 5 cut(s) 151, 186, 229, 267, 319
FbaI TGATCA 4 cut(s) 154, 249, 295, 411
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 439
FokI GGATG 1 cut(s) 367
Fsp4HI GCNGC 1 cut(s) 439
FspBI CTAG 2 cut(s) 393, 417
GluI GCNGC 1 cut(s) 439
HaeIII GGCC 2 cut(s) 34, 63
HapII CCGG 1 cut(s) 64
Hin1II CATG 5 cut(s) 155, 190, 233, 271, 323
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HinfI GANTC 2 cut(s) 195, 217
HpaI GTTAAC 1 cut(s) 327
HpaII CCGG 1 cut(s) 64
HphI GGTGA 2 cut(s) 66, 271
Hpy166II GTNNAC 3 cut(s) 45, 184, 327
Hpy188I TCNGA 3 cut(s) 261, 310, 453
Hpy188III TCNNGA 6 cut(s) 15, 230, 274, 293, 341, 388
Hpy8I GTNNAC 3 cut(s) 45, 184, 327
Hpy99I CGWCG 1 cut(s) 483
HpyAV CCTTC 2 cut(s) 87, 338
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4V TGCA 2 cut(s) 8, 45
HpyF3I CTNAG 1 cut(s) 260
Hsp92II CATG 5 cut(s) 155, 190, 233, 271, 323
Ksp22I TGATCA 4 cut(s) 154, 249, 295, 411
KspAI GTTAAC 1 cut(s) 327
Kzo9I GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
LmnI GCTCC 2 cut(s) 66, 120
LpnPI CCDG 7 cut(s) 28, 77, 117, 144, 306, 344, 427
Lsp1109I GCAGC 1 cut(s) 450
LweI GCATC 4 cut(s) 17, 78, 232, 345
MaeI CTAG 2 cut(s) 393, 417
MaeIII GTNAC 1 cut(s) 463
MalI GATC 7 cut(s) 51, 156, 251, 278, 297, 339, 413
MbiI CCGCTC 1 cut(s) 422
MboI GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
MboII GAAGA 3 cut(s) 119, 156, 347
MfeI CAATTG 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 47, 125
MlsI TGGCCA 1 cut(s) 34
MluCI AATT 3 cut(s) 145, 160, 201
MluNI TGGCCA 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 4 cut(s) 95, 111, 184, 275
Mox20I TGGCCA 1 cut(s) 34
MscI TGGCCA 1 cut(s) 34
MseI TTAA 2 cut(s) 326, 484
Msp20I TGGCCA 1 cut(s) 34
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 132
NdeII GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
NlaIII CATG 5 cut(s) 155, 190, 233, 271, 323
NmuCI GTSAC 1 cut(s) 463
PagI TCATGA 1 cut(s) 229
PfeI GAWTC 2 cut(s) 195, 217
PkrI GCNGC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspPI GGNCC 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 441
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 326, 484
SatI GCNGC 1 cut(s) 439
Sau3AI GATC 7 cut(s) 49, 154, 249, 276, 295, 337, 411
Sau96I GGNCC 1 cut(s) 475
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 2 cut(s) 47, 125
SetI ASST 3 cut(s) 79, 183, 443
SfaNI GCATC 4 cut(s) 17, 78, 232, 345
SinI GGWCC 1 cut(s) 475
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 3 cut(s) 145, 160, 201
SsiI CCGC 1 cut(s) 422
SspMI CTAG 2 cut(s) 393, 417
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 3 cut(s) 101, 374, 471
TaaI ACNGT 1 cut(s) 479
TasI AATT 3 cut(s) 145, 160, 201
TfiI GAWTC 2 cut(s) 195, 217
Tru1I TTAA 2 cut(s) 326, 484
Tru9I TTAA 2 cut(s) 326, 484
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 1 cut(s) 438
Tsp45I GTSAC 1 cut(s) 463
TspRI CASTG 1 cut(s) 52
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 393, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.