Rroxscaffold_5G00369680

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006540
Physical Location & Seq
Reverse (-)
51466515 .. 51468021
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369680.1

Sequence Viewer

Length: 675 bp
ATGGGATTACGAGCTGCTGCACATTCATCATCTTCTTCCATAGACAGCAGCAACCATCCTGACCTTGATGGTTTTCTTCCTAAGCCTTCTGGATCGTCTTCTTGTTCGTCTCTCTCTCACAAAATCAATGGTGGTACTAATTCTCGTAACAGTCGCAGAAGCACTCATACCAGAACAGATTTGAGCACTGATCTCAGACTTGGCCTGAGCATATCACCGTCTCATCACTCTGACTTGTCTTCCACTACTTCAAGCCCAAGGGAAGAAGCATTGGTTAGCTGGCCGCCGATCAAATCGATCTTGAGGAGCACACTTTCAGGCAAATCAGAGAATTGCCATCATCAGAATTCTTCTTTATTTGTGAAGGTCTACATGGAGGGAATCCCAATTGGTAGAAAATTGAATCTGTTTGCTCACGATGGTTATGATGCTTTGATCACAACTCTGGGCCTCATGTTCAAGACCACCATTCTATGTCCTGATAATAATCATGTTCATTCAGAGAAATATTATGTTTTAACTTATGAAGATCAAGAAGGGGATTGGATGCTGGTTGGAGATGTGCCTTGGGAGATATTCTTGACTACTGTGAAAAGACTGAAGATTACTAGAGCAGACAGATGTGAGGTCGATGTTGTCACTACAAGAAAGATTGCTGCCATTGAATGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

224

Amino Acids

24.71

Weight (kDa)

7.17

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 79 - 205 5.8e-34 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AciI CCGC 1 cut(s) 284
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 620
AfaI GTAC 1 cut(s) 136
AgsI TTSAA 4 cut(s) 252, 403, 460, 665
AluBI AGCT 2 cut(s) 14, 279
AluI AGCT 2 cut(s) 14, 279
Alw21I GWGCWC 2 cut(s) 188, 311
Alw26I GTCTC 2 cut(s) 114, 225
AlwI GGATC 1 cut(s) 100
AoxI GGCC 3 cut(s) 202, 281, 448
ApeKI GCWGC 4 cut(s) 14, 17, 48, 656
ApoI RAATTY 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 151, 183
BbsI GAAGAC 2 cut(s) 90, 231
Bbv12I GWGCWC 2 cut(s) 188, 311
BbvI GCAGC 3 cut(s) 4, 60, 643
BccI CCATC 4 cut(s) 62, 63, 345, 413
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 114, 225
BfaI CTAG 1 cut(s) 609
BisI GCNGC 5 cut(s) 15, 18, 49, 284, 657
BlsI GCNGC 5 cut(s) 16, 19, 50, 285, 658
BmgT120I GGNCC 1 cut(s) 448
BmsI GCATC 2 cut(s) 418, 537
BpiI GAAGAC 2 cut(s) 90, 231
Bpu10I CCTNAGC 2 cut(s) 81, 206
BpuEI CTTGAG 1 cut(s) 322
Bsa29I ATCGAT 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 257, 566
Bse8I GATNNNNATC 1 cut(s) 486
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 257, 566
BseGI GGATG 2 cut(s) 55, 552
BseJI GATNNNNATC 1 cut(s) 486
BseMII CTCAG 2 cut(s) 197, 208
BseRI GAGGAG 1 cut(s) 319
BseXI GCAGC 3 cut(s) 4, 60, 643
BshFI GGCC 3 cut(s) 204, 283, 450
BshVI ATCGAT 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 188, 311
BsmAI GTCTC 2 cut(s) 114, 225
BsmBI CGTCTC 2 cut(s) 114, 225
BsnI GGCC 3 cut(s) 204, 283, 450
Bsp1286I GDGCHC 2 cut(s) 188, 311
Bsp143I GATC 6 cut(s) 92, 190, 288, 297, 435, 529
BspACI CCGC 1 cut(s) 284
BspANI GGCC 3 cut(s) 204, 283, 450
BspCNI CTCAG 2 cut(s) 198, 207
BspDI ATCGAT 1 cut(s) 296
BspPI GGATC 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 257, 566
BssMI GATC 6 cut(s) 92, 190, 288, 297, 435, 529
BssT1I CCWWGG 2 cut(s) 257, 566
Bst4CI ACNGT 3 cut(s) 152, 219, 589
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 3 cut(s) 81, 194, 206
BstF5I GGATG 2 cut(s) 55, 552
BstKTI GATC 6 cut(s) 95, 193, 291, 300, 438, 532
BstMAI GTCTC 2 cut(s) 114, 225
BstMBI GATC 6 cut(s) 92, 190, 288, 297, 435, 529
BstV1I GCAGC 3 cut(s) 4, 60, 643
BstV2I GAAGAC 2 cut(s) 90, 231
Bsu15I ATCGAT 1 cut(s) 296
BsuRI GGCC 3 cut(s) 204, 283, 450
BsuTUI ATCGAT 1 cut(s) 296
BtsCI GGATG 2 cut(s) 55, 552
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 448
ClaI ATCGAT 1 cut(s) 296
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 373, 454, 491
CviJI RGCY 7 cut(s) 14, 85, 204, 255, 279, 283, 450
CviKI_1 RGCY 7 cut(s) 14, 85, 204, 255, 279, 283, 450
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 3 cut(s) 81, 194, 206
DpnI GATC 6 cut(s) 94, 192, 290, 299, 437, 531
DpnII GATC 6 cut(s) 92, 190, 288, 297, 435, 529
EaeI YGGCCR 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 257, 566
Eco57I CTGAAG 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 346
EcoT14I CCWWGG 2 cut(s) 257, 566
ErhI CCWWGG 2 cut(s) 257, 566
Esp3I CGTCTC 2 cut(s) 114, 225
FaeI CATG 3 cut(s) 376, 457, 494
FatI CATG 3 cut(s) 372, 453, 490
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 5 cut(s) 15, 18, 49, 284, 657
FokI GGATG 2 cut(s) 42, 559
Fsp4HI GCNGC 5 cut(s) 15, 18, 49, 284, 657
FspBI CTAG 1 cut(s) 609
GluI GCNGC 5 cut(s) 15, 18, 49, 284, 657
HaeIII GGCC 3 cut(s) 204, 283, 450
Hin1II CATG 3 cut(s) 376, 457, 494
HinfI GANTC 2 cut(s) 381, 403
HphI GGTGA 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 5 cut(s) 197, 232, 328, 345, 502
Hpy188III TCNNGA 8 cut(s) 59, 90, 301, 416, 460, 479, 533, 580
Hpy8I GTNNAC 1 cut(s) 370
HpyAV CCTTC 3 cut(s) 96, 358, 530
HpyCH4III ACNGT 3 cut(s) 152, 219, 589
HpyCH4V TGCA 1 cut(s) 20
HpyF3I CTNAG 3 cut(s) 81, 194, 206
Hsp92II CATG 3 cut(s) 376, 457, 494
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 6 cut(s) 92, 190, 288, 297, 435, 529
LmnI GCTCC 1 cut(s) 306
LpnPI CCDG 9 cut(s) 72, 75, 184, 218, 265, 303, 431, 492, 536
Lsp1109I GCAGC 3 cut(s) 4, 60, 643
LweI GCATC 2 cut(s) 418, 537
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 146, 637
MalI GATC 6 cut(s) 94, 192, 290, 299, 437, 531
MboI GATC 6 cut(s) 92, 190, 288, 297, 435, 529
MboII GAAGA 9 cut(s) 24, 27, 68, 90, 231, 275, 342, 539, 613
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 188, 311
MluCI AATT 5 cut(s) 139, 331, 346, 387, 398
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 4 cut(s) 297, 370, 461, 619
MseI TTAA 1 cut(s) 518
MunI CAATTG 1 cut(s) 387
NdeII GATC 6 cut(s) 92, 190, 288, 297, 435, 529
NlaIII CATG 3 cut(s) 376, 457, 494
NmuCI GTSAC 1 cut(s) 637
PfeI GAWTC 2 cut(s) 381, 403
PkrI GCNGC 5 cut(s) 16, 19, 50, 285, 658
PspPI GGNCC 1 cut(s) 448
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 5 cut(s) 15, 18, 49, 284, 657
Sau3AI GATC 6 cut(s) 92, 190, 288, 297, 435, 529
Sau96I GGNCC 1 cut(s) 448
SduI GDGCHC 2 cut(s) 188, 311
SetI ASST 5 cut(s) 16, 66, 281, 369, 630
SfaNI GCATC 2 cut(s) 418, 537
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 5 cut(s) 139, 331, 346, 387, 398
SsiI CCGC 1 cut(s) 284
SspI AATATT 1 cut(s) 509
SspMI CTAG 1 cut(s) 609
StyI CCWWGG 2 cut(s) 257, 566
TaaI ACNGT 3 cut(s) 152, 219, 589
TaqI TCGA 2 cut(s) 296, 630
TasI AATT 5 cut(s) 139, 331, 346, 387, 398
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 2 cut(s) 381, 403
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 14, 17, 48, 656
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 15, 485, 540
TspRI CASTG 1 cut(s) 193
XapI RAATTY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.