Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002303

Physical Location & Seq

Genomic Coordinates Forward (+)

10913576 .. 10918158

Gene Structure 4583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1141100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGGCATCTGGTCTTGATACTGTATTAACCAAGAAGACATTTGTCTTCGGTTTAAAGGTTTGGGTATTAACGGGGATCATTGTGGGAGTTTTTATAGTGATGATTCTTGCGGTGATATCAATTTGTCTTACTTCGCGAAAGAGATCCAGAAAGTCTACTGACATGCTTCCTCTGCGCAGAATTCCAAATGATTCAAAGGAAATCAAAGAGATTAGGGTAGATCAAGCTTGTGCAAATAACTACGTACCCCGTAGTAGTTCTTTTCTCACCCTTGGCGATAAATTCAGTGACAGAGAGTCTGAGAAAGTTTTGATCCATAAGAATGGAGATGGTAGCAGTCGATCAGGCTCATTTAACAATGGGGAGAAGGAAAATGTTGGTTCTCAATCTGGAGATGAGTGTGGTGCAGCTTATATGTATGAGCCTTCTTCACAGCCTATAACTGCACCTTCACCTCTATCTGGTCTACCTGAATTCTCTCGCCTTGGTTGGGGTCACTGGTTTACATTAAGGGACCTTGAGTTCGCAACAGGTCGGTTTTCAAAGGAAAATGTCATTGGTGAGGGTGGATATGGAGTTGTTTATCGGGGTAATCTGATTAATGGATCTCCTGTGGCTGTGAAGAAGCTACTCAACAACCTAGGACAAGCAGAGAAGGAATTCAGAGTGGAAGTTGAGGCTATTGGGCATGTACGACACAAAAACTTGGTTCGACTTTTGGGATACTGCATCGAAGGAACCCACAGGTTGCTGGTTTATGAATATGTCAACAACGGAAATTTGGAGCAATGGCTTCATGGAGCGATGCGTCATCATGGATATCTCACATGGGAGGCACGCATGAAAGTTCTCCTTGGCACCGCTAAAGCGCTGGCTTATTTGCATGAGGCCATTGAACCCAAAGTTGTGCATCGAGACATTAAGTCGAGCAATATCTTACTTGATGATGATTTCAATGCTAAGATATCTGATTTTGGTCTGGCAAAGTTGCTAGGTGCCGGGAAGAGTCATATTACAACTAGAGTTATGGGTACCTTTGGATATGTGGCCCCAGAATATGCCAACTCTGGCCTTCTAAATGAGAAGAGTGATGTTTATAGCTTTGGGGTTGTGATTTTGGAAGCAATCACTGGAAGAGATCCGGTGGATTATGGTCGGCCAGCACAAGAGGTAAATTTGGTGGATTGGCTCAAGATGATGGTTGGAGGCAGGCGTTCAGAAGAGCTGGTAGATCCGAACATTGAGACCAAGCCATCTACGAGTGCCCTTAAACGAGCCCTCTTGACTGCACTGAGGTGTGTAGATCCAGATTCTGATAAAAGGCCTAAGATGGGTCAAGTGGTTCGCATGCTTGAATCGGAGGAGTATCCCATACCTCGAGAGGACCGAAGACGCCGCAGAAGTCAAGCAGGGAACACAGAAGCCGAGTCTCTAAATTCTGATACAGATTGGAGTGAGAAGCCAACTACAAAGACAGATAGCAGAAGGAACCGTCGAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

501

Amino Acids

55.81

Weight (kDa)

9.03

Isoelectric Point (pI)

49.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	181 - 448	6.1e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	183 - 451	9.9e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09440 AT1G09440 AT1G56720 AT1G56720 AT1G56720 AT1G56720
fragaria_vesca	FvH4_4g21450 FvH4_4g21450 FvH4_6g08381
malus_domestica	MD04G1187400.v1.1 MD12G1201000.v1.1 MD13G1141100.v1.1 MD16G1137200.v1.1
prunus_persica	Prupe.1G106300_v2.0.a1 Prupe.1G106300_v2.0.a1 Prupe.6G306000_v2.0.a1 Prupe.6G306000_v2.0.a1 Prupe.6G306000_v2.0.a1
pyrus_communis	pycom04g16550 pycom12g18730 pycom13g12260 pycom16g12090 pycom16g12100
rosa_chinensis	RchiOBHm_Chr3g0458331 RchiOBHm_Chr4g0427381
rosa_laevigata	RLG00000007248 RLG00000025168
rosa_multiflora	Rmu_sc0003813.1_g000020 Rmu_sc0007338.1_g000007
rosa_roxburghii	Rroxscaffold_5G00369050 Rroxscaffold_6G00421160
rosa_rugosa	Rorug03G0025800 Rorug03G0025800 Rorug04G0217200 Rorug04G0217300 Rorug04G0217400 Rorug04G0217500
rosa_samantha	Rh3BG089600 Rh4AG273600 Rh4DG276600
rosa_wichuraiana	Rw3G007360 Rw4G023730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	176
Acc65I	GGTACC	1 cut(s)	1031
AccB1I	GGYRCC	3 cut(s)	857, 995, 1031
AccI	GTMKAC	2 cut(s)	155, 466
AccII	CGCG	1 cut(s)	136
AciI	CCGC	3 cut(s)	110, 861, 1396
AclWI	GGATC	7 cut(s)	83, 138, 307, 613, 1133, 1226, 1298
AcoI	YGGCCR	1 cut(s)	1157
AcsI	RAATTY	7 cut(s)	180, 281, 473, 659, 778, 1174, 1435
AcyI	GRCGYC	1 cut(s)	1393
AfaI	GTAC	3 cut(s)	246, 693, 1033
AfeI	AGCGCT	1 cut(s)	870
AfiI	CCNNNNNNNGG	3 cut(s)	461, 490, 1331
AgsI	TTSAA	5 cut(s)	195, 543, 896, 955, 1355
AhdI	GACNNNNNGTC	2 cut(s)	295, 922
AjuI	GAANNNNNNNTTGG	2 cut(s)	540, 572
AleI	CACNNNNGTG	1 cut(s)	1294
AloI	GAACNNNNNNTCC	2 cut(s)	506, 538
AluBI	AGCT	5 cut(s)	227, 410, 628, 1101, 1225
AluI	AGCT	5 cut(s)	227, 410, 628, 1101, 1225
Alw26I	GTCTC	3 cut(s)	909, 1238, 1434
AlwI	GGATC	7 cut(s)	83, 138, 307, 613, 1133, 1226, 1298
AlwNI	CAGNNNCTG	1 cut(s)	1313
Ama87I	CYCGRG	1 cut(s)	1377
Aor51HI	AGCGCT	1 cut(s)	870
AoxI	GGCC	5 cut(s)	888, 1047, 1069, 1157, 1322
ApeKI	GCWGC	1 cut(s)	407
ApoI	RAATTY	7 cut(s)	180, 281, 473, 659, 778, 1174, 1435
ArsI	GACNNNNNNTTYG	2 cut(s)	506, 538
AseI	ATTAAT	1 cut(s)	600
Asp700I	GAANNNNTTC	1 cut(s)	659
Asp718I	GGTACC	1 cut(s)	1031
AspA2I	CCTAGG	1 cut(s)	640
AspLEI	GCGC	2 cut(s)	177, 871
AspS9I	GGNCC	3 cut(s)	514, 1048, 1384
AsuC2I	CCSGG	1 cut(s)	1000
AsuHPI	GGTGA	4 cut(s)	124, 259, 444, 572
AvaI	CYCGRG	1 cut(s)	1377
AvaII	GGWCC	2 cut(s)	514, 1384
AvrII	CCTAGG	1 cut(s)	640
BaeGI	GKGCMC	1 cut(s)	1267
BanI	GGYRCC	3 cut(s)	857, 995, 1031
BanII	GRGCYC	1 cut(s)	1279
BbsI	GAAGAC	3 cut(s)	37, 41, 1396
BbvI	GCAGC	1 cut(s)	419
BccI	CCATC	4 cut(s)	323, 1192, 1261, 1324
BciVI	GTATCC	2 cut(s)	716, 1377
BcnI	CCSGG	1 cut(s)	1000
BcoDI	GTCTC	3 cut(s)	909, 1238, 1434
BfaI	CTAG	3 cut(s)	641, 992, 1020
BfoI	RGCGCY	1 cut(s)	872
BfuI	GTATCC	2 cut(s)	716, 1377
BisI	GCNGC	2 cut(s)	408, 1396
BlnI	CCTAGG	1 cut(s)	640
BlsI	GCNGC	2 cut(s)	409, 1397
Bme1390I	CCNGG	1 cut(s)	1000
Bme18I	GGWCC	2 cut(s)	514, 1384
BmeRI	GACNNNNNGTC	2 cut(s)	295, 922
BmeT110I	CYCGRG	1 cut(s)	1377
BmgT120I	GGNCC	3 cut(s)	514, 1048, 1384
BmiI	GGNNCC	7 cut(s)	515, 739, 859, 997, 1033, 1050, 1490
BmrFI	CCNGG	1 cut(s)	1000
BmsI	GCATC	4 cut(s)	14, 738, 795, 919
BoxI	GACNNNNGTC	1 cut(s)	41
BpiI	GAAGAC	3 cut(s)	37, 41, 1396
BpmI	CTGGAG	1 cut(s)	411
BpuEI	CTTGAG	2 cut(s)	539, 1175
BpuMI	CCSGG	1 cut(s)	1000
BsaAI	YACGTR	1 cut(s)	244
BsaHI	GRCGYC	1 cut(s)	1393
BsaI	GGTCTC	1 cut(s)	1238
BsaJI	CCNNGG	4 cut(s)	271, 484, 640, 853
BsaWI	WCCGGW	1 cut(s)	1141
Bsc4I	CCNNNNNNNGG	3 cut(s)	461, 490, 1331
Bse1I	ACTGG	2 cut(s)	503, 1135
Bse3DI	GCAATG	1 cut(s)	794
BseDI	CCNNGG	4 cut(s)	271, 484, 640, 853
BseLI	CCNNNNNNNGG	3 cut(s)	461, 490, 1331
BseMI	GCAATG	1 cut(s)	794
BseMII	CTCAG	2 cut(s)	291, 1283
BseNI	ACTGG	2 cut(s)	503, 1135
BseRI	GAGGAG	1 cut(s)	1376
BseSI	GKGCMC	1 cut(s)	1267
BseXI	GCAGC	1 cut(s)	419
BsgI	GTGCAG	3 cut(s)	426, 429, 1272
Bsh1236I	CGCG	1 cut(s)	136
BshFI	GGCC	5 cut(s)	890, 1049, 1071, 1159, 1324
BshNI	GGYRCC	3 cut(s)	857, 995, 1031
BsiHKCI	CYCGRG	1 cut(s)	1377
BsiSI	CCGG	2 cut(s)	999, 1142
BslFI	GGGAC	1 cut(s)	527
BslI	CCNNNNNNNGG	3 cut(s)	461, 490, 1331
BsmAI	GTCTC	3 cut(s)	909, 1238, 1434
BsmFI	GGGAC	1 cut(s)	527
BsnI	GGCC	5 cut(s)	890, 1049, 1071, 1159, 1324
Bso31I	GGTCTC	1 cut(s)	1238
BsoBI	CYCGRG	1 cut(s)	1377
Bsp1286I	GDGCHC	2 cut(s)	1267, 1279
Bsp143I	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
Bsp68I	TCGCGA	1 cut(s)	136
BspACI	CCGC	3 cut(s)	110, 861, 1396
BspANI	GGCC	5 cut(s)	890, 1049, 1071, 1159, 1324
BspCNI	CTCAG	2 cut(s)	292, 1284
BspFNI	CGCG	1 cut(s)	136
BspLI	GGNNCC	7 cut(s)	515, 739, 859, 997, 1033, 1050, 1490
BspPI	GGATC	7 cut(s)	83, 138, 307, 613, 1133, 1226, 1298
BspQI	GCTCTTC	1 cut(s)	1215
BspT107I	GGYRCC	3 cut(s)	857, 995, 1031
BspTNI	GGTCTC	1 cut(s)	1238
BsrDI	GCAATG	1 cut(s)	794
BsrI	ACTGG	2 cut(s)	503, 1135
BssECI	CCNNGG	4 cut(s)	271, 484, 640, 853
BssMI	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
BssNI	GRCGYC	1 cut(s)	1393
BssT1I	CCWWGG	4 cut(s)	271, 484, 640, 853
Bst4CI	ACNGT	2 cut(s)	22, 1493
Bst6I	CTCTTC	4 cut(s)	998, 1079, 1129, 1215
BstACI	GRCGYC	1 cut(s)	1393
BstBAI	YACGTR	1 cut(s)	244
BstC8I	GCNNGC	5 cut(s)	838, 873, 1161, 1211, 1349
BstDEI	CTNAG	4 cut(s)	300, 960, 1292, 1326
BstFNI	CGCG	1 cut(s)	136
BstH2I	RGCGCY	1 cut(s)	872
BstHHI	GCGC	2 cut(s)	177, 871
BstKTI	GATC	9 cut(s)	78, 146, 223, 315, 344, 608, 1141, 1234, 1306
BstMAI	GTCTC	3 cut(s)	909, 1238, 1434
BstMBI	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
BstMWI	GCNNNNNNNGC	1 cut(s)	172
BstNSI	RCATGY	3 cut(s)	166, 692, 1351
BstPAI	GACNNNNGTC	1 cut(s)	41
BstSCI	CCNGG	1 cut(s)	998
BstSLI	GKGCMC	1 cut(s)	1267
BstSNI	TACGTA	1 cut(s)	244
BstUI	CGCG	1 cut(s)	136
BstV1I	GCAGC	1 cut(s)	419
BstV2I	GAAGAC	3 cut(s)	37, 41, 1396
BstX2I	RGATCY	5 cut(s)	143, 605, 1138, 1231, 1303
BstXI	CCANNNNNNTGG	1 cut(s)	323
BstYI	RGATCY	5 cut(s)	143, 605, 1138, 1231, 1303
BsuI	GTATCC	2 cut(s)	716, 1377
BsuRI	GGCC	5 cut(s)	890, 1049, 1071, 1159, 1324
BtgZI	GCGATG	1 cut(s)	818
BtsIMutI	CAGTG	4 cut(s)	292, 496, 1128, 1289
BtuMI	TCGCGA	1 cut(s)	136
Cac8I	GCNNGC	5 cut(s)	838, 873, 1161, 1211, 1349
CaiI	CAGNNNCTG	1 cut(s)	1313
CfoI	GCGC	2 cut(s)	177, 871
Cfr13I	GGNCC	3 cut(s)	514, 1048, 1384
CseI	GACGC	2 cut(s)	797, 1401
Csp6I	GTAC	3 cut(s)	245, 692, 1032
CviAII	CATG	8 cut(s)	163, 689, 797, 815, 828, 841, 884, 1348
CviQI	GTAC	3 cut(s)	245, 692, 1032
DdeI	CTNAG	4 cut(s)	300, 960, 1292, 1326
DpnI	GATC	9 cut(s)	77, 145, 222, 314, 343, 607, 1140, 1233, 1305
DpnII	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
DraI	TTTAAA	1 cut(s)	54
DriI	GACNNNNNGTC	2 cut(s)	295, 922
EaeI	YGGCCR	1 cut(s)	1157
Eam1104I	CTCTTC	4 cut(s)	998, 1079, 1129, 1215
Eam1105I	GACNNNNNGTC	2 cut(s)	295, 922
EarI	CTCTTC	4 cut(s)	998, 1079, 1129, 1215
Eco105I	TACGTA	1 cut(s)	244
Eco130I	CCWWGG	4 cut(s)	271, 484, 640, 853
Eco147I	AGGCCT	1 cut(s)	1324
Eco24I	GRGCYC	1 cut(s)	1279
Eco31I	GGTCTC	1 cut(s)	1238
Eco32I	GATATC	3 cut(s)	117, 821, 966
Eco47I	GGWCC	2 cut(s)	514, 1384
Eco47III	AGCGCT	1 cut(s)	870
Eco88I	CYCGRG	1 cut(s)	1377
EcoO109I	RGGNCCY	1 cut(s)	514
EcoRI	GAATTC	3 cut(s)	180, 473, 659
EcoRV	GATATC	3 cut(s)	117, 821, 966
EcoT14I	CCWWGG	4 cut(s)	271, 484, 640, 853
EcoT38I	GRGCYC	1 cut(s)	1279
ErhI	CCWWGG	4 cut(s)	271, 484, 640, 853
FaeI	CATG	8 cut(s)	166, 692, 800, 818, 831, 844, 887, 1351
FalI	AAGNNNNNCTT	2 cut(s)	837, 869
FaqI	GGGAC	1 cut(s)	527
FatI	CATG	8 cut(s)	162, 688, 796, 814, 827, 840, 883, 1347
FblI	GTMKAC	2 cut(s)	155, 466
Fnu4HI	GCNGC	2 cut(s)	408, 1396
FriOI	GRGCYC	1 cut(s)	1279
Fsp4HI	GCNGC	2 cut(s)	408, 1396
FspBI	CTAG	3 cut(s)	641, 992, 1020
FspI	TGCGCA	1 cut(s)	176
GlaI	GCGC	2 cut(s)	176, 870
GluI	GCNGC	2 cut(s)	408, 1396
GsuI	CTGGAG	1 cut(s)	411
HaeII	RGCGCY	1 cut(s)	872
HaeIII	GGCC	5 cut(s)	890, 1049, 1071, 1159, 1324
HapII	CCGG	2 cut(s)	999, 1142
HgaI	GACGC	2 cut(s)	797, 1401
HhaI	GCGC	2 cut(s)	177, 871
Hin1I	GRCGYC	1 cut(s)	1393
Hin1II	CATG	8 cut(s)	166, 692, 800, 818, 831, 844, 887, 1351
Hin6I	GCGC	2 cut(s)	175, 869
HinP1I	GCGC	2 cut(s)	175, 869
HincII	GTYRAC	1 cut(s)	769
HindII	GTYRAC	1 cut(s)	769
HindIII	AAGCTT	1 cut(s)	225
HinfI	GANTC	7 cut(s)	103, 191, 296, 1006, 1310, 1355, 1427
HpaII	CCGG	2 cut(s)	999, 1142
HphI	GGTGA	4 cut(s)	124, 259, 444, 572
Hpy166II	GTNNAC	4 cut(s)	156, 467, 504, 769
Hpy188I	TCNGA	9 cut(s)	301, 597, 665, 970, 1219, 1236, 1315, 1360, 1441
Hpy188III	TCNNGA	9 cut(s)	14, 135, 147, 390, 914, 1192, 1282, 1307, 1379
Hpy8I	GTNNAC	4 cut(s)	156, 467, 504, 769
Hpy99I	CGWCG	1 cut(s)	1497
HpyAV	CCTTC	7 cut(s)	361, 435, 459, 649, 728, 1082, 1479
HpyCH4III	ACNGT	2 cut(s)	22, 1493
HpyCH4IV	ACGT	1 cut(s)	243
HpyCH4V	TGCA	7 cut(s)	233, 407, 446, 729, 883, 910, 1289
HpyF10VI	GCNNNNNNNGC	1 cut(s)	172
HpyF3I	CTNAG	4 cut(s)	300, 960, 1292, 1326
HpySE526I	ACGT	1 cut(s)	243
Hsp92I	GRCGYC	1 cut(s)	1393
Hsp92II	CATG	8 cut(s)	166, 692, 800, 818, 831, 844, 887, 1351
HspAI	GCGC	2 cut(s)	175, 869
KpnI	GGTACC	1 cut(s)	1035
Kzo9I	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
LguI	GCTCTTC	1 cut(s)	1215
LmnI	GCTCC	2 cut(s)	784, 800
Lsp1109I	GCAGC	1 cut(s)	419
LweI	GCATC	4 cut(s)	14, 738, 795, 919
MaeI	CTAG	3 cut(s)	641, 992, 1020
MaeII	ACGT	1 cut(s)	243
MaeIII	GTNAC	2 cut(s)	287, 494
MalI	GATC	9 cut(s)	77, 145, 222, 314, 343, 607, 1140, 1233, 1305
MboI	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
MboII	GAAGA	9 cut(s)	37, 46, 420, 634, 1015, 1096, 1146, 1232, 1401
MflI	RGATCY	5 cut(s)	143, 605, 1138, 1231, 1303
MhlI	GDGCHC	2 cut(s)	1267, 1279
MluCI	AATT	8 cut(s)	120, 180, 281, 473, 659, 778, 1174, 1435
MlyI	GAGTC	3 cut(s)	305, 1015, 1436
MmeI	TCCRAC	1 cut(s)	1183
MroXI	GAANNNNTTC	1 cut(s)	659
MseI	TTAA	8 cut(s)	26, 53, 68, 354, 509, 600, 921, 1269
MslI	CAYNNNNRTG	2 cut(s)	321, 1294
MspI	CCGG	2 cut(s)	999, 1142
MspR9I	CCNGG	1 cut(s)	1000
MvnI	CGCG	1 cut(s)	136
MwoI	GCNNNNNNNGC	1 cut(s)	172
NciI	CCSGG	1 cut(s)	1000
NdeII	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
NlaIII	CATG	8 cut(s)	166, 692, 800, 818, 831, 844, 887, 1351
NlaIV	GGNNCC	7 cut(s)	515, 739, 859, 997, 1033, 1050, 1490
NmeAIII	GCCGAG	1 cut(s)	1450
NmuCI	GTSAC	2 cut(s)	287, 494
NruI	TCGCGA	1 cut(s)	136
NsbI	TGCGCA	1 cut(s)	176
NspI	RCATGY	3 cut(s)	166, 692, 1351
OliI	CACNNNNGTG	1 cut(s)	1294
PaeI	GCATGC	1 cut(s)	1351
PaeR7I	CTCGAG	1 cut(s)	1377
PceI	AGGCCT	1 cut(s)	1324
PciSI	GCTCTTC	1 cut(s)	1215
PcsI	WCGNNNNNNNCGW	1 cut(s)	1384
PdmI	GAANNNNTTC	1 cut(s)	659
PfeI	GAWTC	4 cut(s)	103, 191, 1310, 1355
PkrI	GCNGC	2 cut(s)	409, 1397
PleI	GAGTC	3 cut(s)	304, 1014, 1435
PpsI	GAGTC	3 cut(s)	304, 1014, 1435
Ppu21I	YACGTR	1 cut(s)	244
PpuMI	RGGWCCY	1 cut(s)	514
PshAI	GACNNNNGTC	1 cut(s)	41
PshBI	ATTAAT	1 cut(s)	600
Psp5II	RGGWCCY	1 cut(s)	514
PspN4I	GGNNCC	7 cut(s)	515, 739, 859, 997, 1033, 1050, 1490
PspPI	GGNCC	3 cut(s)	514, 1048, 1384
PspPPI	RGGWCCY	1 cut(s)	514
PstNI	CAGNNNCTG	1 cut(s)	1313
PsuI	RGATCY	5 cut(s)	143, 605, 1138, 1231, 1303
RruI	TCGCGA	1 cut(s)	136
RsaI	GTAC	3 cut(s)	246, 693, 1033
RsaNI	GTAC	3 cut(s)	245, 692, 1032
RseI	CAYNNNNRTG	2 cut(s)	321, 1294
SapI	GCTCTTC	1 cut(s)	1215
SaqAI	TTAA	8 cut(s)	26, 53, 68, 354, 509, 600, 921, 1269
SatI	GCNGC	2 cut(s)	408, 1396
Sau3AI	GATC	9 cut(s)	75, 143, 220, 312, 341, 605, 1138, 1231, 1303
Sau96I	GGNCC	3 cut(s)	514, 1048, 1384
SchI	GAGTC	3 cut(s)	305, 1015, 1436
ScrFI	CCNGG	1 cut(s)	1000
SduI	GDGCHC	2 cut(s)	1267, 1279
SfaNI	GCATC	4 cut(s)	14, 738, 795, 919
Sfr274I	CTCGAG	1 cut(s)	1377
SinI	GGWCC	2 cut(s)	514, 1384
SlaI	CTCGAG	1 cut(s)	1377
SmiMI	CAYNNNNRTG	2 cut(s)	321, 1294
SmlI	CTYRAG	3 cut(s)	518, 1190, 1377
SmoI	CTYRAG	3 cut(s)	518, 1190, 1377
SnaBI	TACGTA	1 cut(s)	244
SphI	GCATGC	1 cut(s)	1351
Sse9I	AATT	8 cut(s)	120, 180, 281, 473, 659, 778, 1174, 1435
SseBI	AGGCCT	1 cut(s)	1324
SsiI	CCGC	3 cut(s)	110, 861, 1396
SspMI	CTAG	3 cut(s)	641, 992, 1020
StuI	AGGCCT	1 cut(s)	1324
StyD4I	CCNGG	1 cut(s)	998
StyI	CCWWGG	4 cut(s)	271, 484, 640, 853
TaaI	ACNGT	2 cut(s)	22, 1493
TaiI	ACGT	1 cut(s)	246
TaqI	TCGA	7 cut(s)	340, 712, 732, 913, 926, 1378, 1495
TaqII	GACCGA	1 cut(s)	1401
TasI	AATT	8 cut(s)	120, 180, 281, 473, 659, 778, 1174, 1435
TauI	GCSGC	1 cut(s)	1398
TfiI	GAWTC	4 cut(s)	103, 191, 1310, 1355
Tru1I	TTAA	8 cut(s)	26, 53, 68, 354, 509, 600, 921, 1269
Tru9I	TTAA	8 cut(s)	26, 53, 68, 354, 509, 600, 921, 1269
TscAI	CASTG	4 cut(s)	292, 503, 1135, 1296
TseFI	GTSAC	2 cut(s)	287, 494
TseI	GCWGC	1 cut(s)	407
Tsp45I	GTSAC	2 cut(s)	287, 494
TspDTI	ATGAA	3 cut(s)	774, 785, 857
TspGWI	ACGGA	1 cut(s)	789
TspRI	CASTG	4 cut(s)	292, 503, 1135, 1296
VpaK11BI	GGWCC	2 cut(s)	514, 1384
VspI	ATTAAT	1 cut(s)	600
XapI	RAATTY	7 cut(s)	180, 281, 473, 659, 778, 1174, 1435
XceI	RCATGY	3 cut(s)	166, 692, 1351
XhoI	CTCGAG	1 cut(s)	1377
XmaJI	CCTAGG	1 cut(s)	640
XmiI	GTMKAC	2 cut(s)	155, 466
XmnI	GAANNNNTTC	1 cut(s)	659
XspI	CTAG	3 cut(s)	641, 992, 1020

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.