MD13G1198000.v1.1

Belongs to the eukaryotic ribosomal protein eS1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004695
Physical Location & Seq
Reverse (-)
17460055 .. 17462838
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1198000.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGCCGTCGGCAAGAACAAGAGGATTTCCAAGTCGAAGAAAGGAGGAAAGAAGAAAGCGGTTGATCCGTTTGCCAAGAAGGATTGGTACGACATCAAGGCCCCGAAACTGTTCAACAACACGAATGTGGGCAAAACTCTCGTCTCCCGTACTCAGGGTACCAAGATTGCTTCCGAAGGACTCAAGCATAGAGTCTTTGAGGTATCACTCGCTGACCTTCAGGGTGATGAGGAGCATGCTTACAGAAAGATGCGGTTGAGAGCTGAAGATGTCCAAGGGAAGACTGTTCTTACTAATTTCTGGGGAATGAACTTCACCACTGACAAGCTTAGGTCTTTGGTGCGCAAATGGCAAACCTTGATTGAAGCTCATGTGGATGTGAAGACCACTGACAACTATACTCTAAGGATGTTCTGCATTGGCTTCACCAAGAGACGTCCAAATCAGGTCAAGAGGACCTGCTATGCACAGTCTAGCCAAGTCAGACAGATTCGCCGTAAGATGAGTGAGATCATGATTGCCCAGGCAACATCAAGTGATCTTAAGGGCTTGGTCAACAAGTTCATTGCTGAGAGCATTGGTAAAGAGATTGAGAAGGCAACAATGAGCATCTATCCATTGCAAAATGTCTTCATCAGGAAGGTCAAGATCTTGAAGGCACCCAAGTTTGATCTCACCAAGCTCATGGAGGTTTATGGTGGCGAAGATGTTGGTGTGAAGGTTGAGAGGCCCGCCGAAGAAGCAGCGCCTGAGGTGCAGCAAGAAATCGTTGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S3Ae

Protein Analysis

260

Amino Acids

29.43

Weight (kDa)

9.94

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S3Ae PF01015 16 - 221 9.8e-81 Ribosomal S3Ae family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 439
Acc16I TGCGCA 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 467
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 2 cut(s) 158, 658
AciI CCGC 3 cut(s) 59, 253, 732
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 203, 285
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 3 cut(s) 89, 151, 160
AfiI CCNNNNNNNGG 1 cut(s) 154
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 3 cut(s) 115, 365, 655
AjnI CCWGG 1 cut(s) 522
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 4 cut(s) 263, 328, 368, 682
AluI AGCT 4 cut(s) 263, 328, 368, 682
Alw26I GTCTC 2 cut(s) 148, 427
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 3, 99, 728
ApeKI GCWGC 2 cut(s) 743, 757
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 158
AspLEI GCGC 2 cut(s) 345, 748
AspS9I GGNCC 3 cut(s) 100, 456, 729
AsuHPI GGTGA 4 cut(s) 236, 307, 418, 667
AvaII GGWCC 1 cut(s) 456
AxyI CCTNAGG 1 cut(s) 750
BanI GGYRCC 2 cut(s) 158, 658
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 3 cut(s) 287, 389, 622
BbvI GCAGC 2 cut(s) 755, 769
BceAI ACGGC 1 cut(s) 480
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 1 cut(s) 524
BcoDI GTCTC 2 cut(s) 148, 427
BfaI CTAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 749
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 648
BisI GCNGC 2 cut(s) 744, 758
BlsI GCNGC 2 cut(s) 745, 759
Bme1390I CCNGG 1 cut(s) 524
Bme18I GGWCC 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 100, 456, 729
BmiI GGNNCC 3 cut(s) 102, 160, 660
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 2 cut(s) 240, 618
BpiI GAAGAC 3 cut(s) 287, 389, 622
Bpu10I CCTNAGC 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 274, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse21I CCTNAGG 1 cut(s) 750
Bse3DI GCAATG 2 cut(s) 564, 617
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 2 cut(s) 274, 522
BseGI GGATG 2 cut(s) 382, 414
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 2 cut(s) 564, 617
BseMII CTCAG 3 cut(s) 167, 561, 741
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 2 cut(s) 755, 769
BsgI GTGCAG 1 cut(s) 776
BshFI GGCC 3 cut(s) 5, 101, 730
BshNI GGYRCC 2 cut(s) 158, 658
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 148, 427
BsmBI CGTCTC 2 cut(s) 148, 427
BsnI GGCC 3 cut(s) 5, 101, 730
Bsp143I GATC 5 cut(s) 64, 510, 538, 648, 670
BspACI CCGC 3 cut(s) 59, 253, 732
BspANI GGCC 3 cut(s) 5, 101, 730
BspCNI CTCAG 3 cut(s) 166, 562, 742
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 3 cut(s) 102, 160, 660
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 158, 658
BspTI CTTAAG 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 564, 617
BssECI CCNNGG 2 cut(s) 274, 522
BssMI GATC 5 cut(s) 64, 510, 538, 648, 670
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 3 cut(s) 111, 286, 471
BstACI GRCGYC 1 cut(s) 436
BstAFI CTTAAG 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 237, 732
BstDEI CTNAG 5 cut(s) 153, 329, 404, 570, 750
BstF5I GGATG 2 cut(s) 382, 414
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 2 cut(s) 345, 748
BstKTI GATC 5 cut(s) 67, 513, 541, 651, 673
BstMAI GTCTC 2 cut(s) 148, 427
BstMBI GATC 5 cut(s) 64, 510, 538, 648, 670
BstMWI GCNNNNNNNGC 3 cut(s) 349, 740, 754
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 755, 769
BstV2I GAAGAC 3 cut(s) 287, 389, 622
BstX2I RGATCY 1 cut(s) 648
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 1 cut(s) 648
Bsu36I CCTNAGG 1 cut(s) 750
BsuRI GGCC 3 cut(s) 5, 101, 730
BtsCI GGATG 2 cut(s) 382, 414
BtsIMutI CAGTG 2 cut(s) 318, 387
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 237, 732
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 513
CfoI GCGC 2 cut(s) 345, 748
Cfr13I GGNCC 3 cut(s) 100, 456, 729
Csp6I GTAC 3 cut(s) 88, 150, 159
CviAII CATG 4 cut(s) 236, 371, 514, 685
CviQI GTAC 3 cut(s) 88, 150, 159
DdeI CTNAG 5 cut(s) 153, 329, 404, 570, 750
DpnI GATC 5 cut(s) 66, 512, 540, 650, 672
DpnII GATC 5 cut(s) 64, 510, 538, 648, 670
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 203, 285
Eco81I CCTNAGG 1 cut(s) 750
EcoO109I RGGNCCY 2 cut(s) 100, 456
EcoRII CCWGG 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 2 cut(s) 148, 427
FaeI CATG 4 cut(s) 239, 374, 517, 688
FaiI YATR 8 cut(s) 189, 237, 372, 399, 465, 515, 686, 696
FatI CATG 4 cut(s) 235, 370, 513, 684
FauI CCCGC 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 744, 758
FokI GGATG 2 cut(s) 389, 421
Fsp4HI GCNGC 2 cut(s) 744, 758
FspBI CTAG 1 cut(s) 474
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 2 cut(s) 344, 747
GluI GCNGC 2 cut(s) 744, 758
HaeII RGCGCY 1 cut(s) 749
HaeIII GGCC 3 cut(s) 5, 101, 730
HhaI GCGC 2 cut(s) 345, 748
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 4 cut(s) 239, 374, 517, 688
Hin6I GCGC 2 cut(s) 343, 746
HinP1I GCGC 2 cut(s) 343, 746
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 180, 192, 490
HphI GGTGA 4 cut(s) 236, 307, 418, 667
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 2 cut(s) 175, 485
Hpy188III TCNNGA 5 cut(s) 451, 514, 637, 646, 652
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 7 cut(s) 73, 170, 227, 589, 634, 649, 712
HpyCH4III ACNGT 3 cut(s) 111, 286, 471
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 4 cut(s) 417, 467, 622, 757
HpyF10VI GCNNNNNNNGC 3 cut(s) 349, 740, 754
HpyF3I CTNAG 5 cut(s) 153, 329, 404, 570, 750
HpySE526I ACGT 1 cut(s) 436
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 4 cut(s) 239, 374, 517, 688
HspAI GCGC 2 cut(s) 343, 746
KpnI GGTACC 1 cut(s) 162
Kzo9I GATC 5 cut(s) 64, 510, 538, 648, 670
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 9 cut(s) 140, 206, 286, 431, 472, 509, 536, 622, 762
Lsp1109I GCAGC 2 cut(s) 755, 769
LweI GCATC 2 cut(s) 240, 618
MaeI CTAG 1 cut(s) 474
MaeII ACGT 1 cut(s) 436
MalI GATC 5 cut(s) 66, 512, 540, 650, 672
MboI GATC 5 cut(s) 64, 510, 538, 648, 670
MboII GAAGA 8 cut(s) 49, 64, 278, 292, 394, 622, 716, 749
MflI RGATCY 1 cut(s) 648
MluCI AATT 1 cut(s) 295
MlyI GAGTC 2 cut(s) 174, 201
MnlI CCTC 8 cut(s) 15, 38, 193, 223, 447, 682, 720, 745
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 2 cut(s) 543, 778
MslI CAYNNNNRTG 2 cut(s) 125, 375
MspCI CTTAAG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 524
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 349, 740, 754
NdeII GATC 5 cut(s) 64, 510, 538, 648, 670
NlaIII CATG 4 cut(s) 239, 374, 517, 688
NlaIV GGNNCC 3 cut(s) 102, 160, 660
NsbI TGCGCA 1 cut(s) 344
NspI RCATGY 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 125
PaeI GCATGC 1 cut(s) 239
PagI TCATGA 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 490
PkrI GCNGC 2 cut(s) 745, 759
PleI GAGTC 2 cut(s) 174, 200
PpsI GAGTC 2 cut(s) 174, 200
PpuMI RGGWCCY 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 3 cut(s) 102, 160, 660
PspPI GGNCC 3 cut(s) 100, 456, 729
PspPPI RGGWCCY 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 648
RsaI GTAC 3 cut(s) 89, 151, 160
RsaNI GTAC 3 cut(s) 88, 150, 159
RseI CAYNNNNRTG 2 cut(s) 125, 375
SaqAI TTAA 2 cut(s) 543, 778
SatI GCNGC 2 cut(s) 744, 758
Sau3AI GATC 5 cut(s) 64, 510, 538, 648, 670
Sau96I GGNCC 3 cut(s) 100, 456, 729
SchI GAGTC 2 cut(s) 174, 201
ScrFI CCNGG 1 cut(s) 524
SfaNI GCATC 2 cut(s) 240, 618
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 2 cut(s) 125, 375
SmlI CTYRAG 2 cut(s) 182, 542
SmoI CTYRAG 2 cut(s) 182, 542
SphI GCATGC 1 cut(s) 239
Sse9I AATT 1 cut(s) 295
SsiI CCGC 3 cut(s) 59, 253, 732
SspMI CTAG 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 3 cut(s) 111, 286, 471
TaiI ACGT 1 cut(s) 439
TaqI TCGA 1 cut(s) 35
TasI AATT 1 cut(s) 295
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 2 cut(s) 543, 778
Tru9I TTAA 2 cut(s) 543, 778
TscAI CASTG 2 cut(s) 325, 394
TseI GCWGC 2 cut(s) 743, 757
TspDTI ATGAA 3 cut(s) 323, 553, 622
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 2 cut(s) 325, 394
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 456
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 474
ZraI GACGTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.