Rh4DG012000

Belongs to the eukaryotic ribosomal protein eS1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004695
Physical Location & Seq
Reverse (-)
1927032 .. 1929391
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG012000.1

Sequence Viewer

Length: 780 bp
ATGGCCGTCGGGAAGAACAAGAGAATCTCCAAGGGCAAGAAGGGAGGAAAGAAGAAAGCAGCTGATCCTTTTGCCAAGAAGGACTGGTATGACATCAAGGCTCCTTCAATCTTCAACGTCAAGCAGGTCGGAAAGACTCTCGTCACCCGTACTCAGGGTACCAAGATTGCTTCCGAAGGACTCAAGCACAGAGTCTTTGAGACATCACTTGCTGACCTTCAGGGTGATGAGGAGCATGCTTTCAGAAAGATGCGATTGAGAGCTGAAGATGTTCAAGGAAGGACTGTTCTGACAAACTTCTGGGGAATGAACTTCACAACTGACAAGTTGAGATCTTTGGTGCGCAAATGGCAAACCTTGATTGAAGCTCATGTGGATGTGAAGACCACAGACAACTACACACTGAGGATGTTCTGCATTGGATTCACCAAGAGACGTCCTAATCAAGTCAAGAGGACCTGTTATGCTCAGTCTAGCCAGATTAGACAGATCCGCCGCAAGATGACCGAGATTATGGTTGCCCAGGCAACATCTTGTGATCTCAAGGATTTGGTCCGCAAGTTCATTGCTGAGAGTATTGGTAAAGATATTGAGAAGGCAACTACAAGCATCTATCCTCTACAAAATGTCTTCATCAGGAAGGTCAAGATCTTAAAGGCACCCAAGTTTGATCTTGGAAAGCTTATGGAGGTTCATGGTGATGAAGATGTTGGGACCAAGGTTGAGAGGCCTGCTGAAGAAGCAGTCCCTGAGGCACCAGTTGAGATTGTTGGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S3Ae

Protein Analysis

259

Amino Acids

29.4

Weight (kDa)

9.91

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S3Ae PF01015 16 - 221 5.2e-81 Ribosomal S3Ae family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 439
Acc16I TGCGCA 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 115
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 3 cut(s) 158, 658, 754
AciI CCGC 3 cut(s) 493, 496, 556
AclWI GGATC 2 cut(s) 59, 484
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 203, 285, 756
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 2 cut(s) 151, 160
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 4 cut(s) 108, 115, 275, 365
AjnI CCWGG 1 cut(s) 522
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 5 cut(s) 62, 263, 368, 682, 776
AluI AGCT 5 cut(s) 62, 263, 368, 682, 776
Alw26I GTCTC 2 cut(s) 194, 427
AlwI GGATC 2 cut(s) 59, 484
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 2 cut(s) 3, 728
ApeKI GCWGC 1 cut(s) 59
Asp700I GAANNNNTTC 1 cut(s) 270
Asp718I GGTACC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 3 cut(s) 456, 553, 714
AsuHPI GGTGA 4 cut(s) 136, 236, 418, 710
AvaII GGWCC 3 cut(s) 456, 553, 714
AxyI CCTNAGG 1 cut(s) 750
BanI GGYRCC 3 cut(s) 158, 658, 754
BbsI GAAGAC 2 cut(s) 389, 622
BbvI GCAGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 524
BcoDI GTCTC 2 cut(s) 194, 427
BfaI CTAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 115
BglII AGATCT 2 cut(s) 332, 648
BisI GCNGC 2 cut(s) 60, 496
BlsI GCNGC 2 cut(s) 61, 497
Bme1390I CCNGG 1 cut(s) 524
Bme18I GGWCC 3 cut(s) 456, 553, 714
BmgT120I GGNCC 3 cut(s) 456, 553, 714
BmiI GGNNCC 5 cut(s) 102, 160, 660, 715, 756
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 2 cut(s) 240, 618
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 389, 622
BpuEI CTTGAG 2 cut(s) 167, 527
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 30, 522, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 2 cut(s) 89, 758
Bse21I CCTNAGG 1 cut(s) 750
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 3 cut(s) 30, 522, 717
BseGI GGATG 2 cut(s) 382, 414
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 5 cut(s) 167, 395, 482, 561, 741
BseNI ACTGG 2 cut(s) 89, 758
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 1 cut(s) 71
BshFI GGCC 2 cut(s) 5, 730
BshNI GGYRCC 3 cut(s) 158, 658, 754
BslFI GGGAC 2 cut(s) 727, 731
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 194, 427
BsmBI CGTCTC 1 cut(s) 427
BsmFI GGGAC 2 cut(s) 727, 731
BsnI GGCC 2 cut(s) 5, 730
Bsp143I GATC 6 cut(s) 64, 332, 489, 538, 648, 670
BspACI CCGC 3 cut(s) 493, 496, 556
BspANI GGCC 2 cut(s) 5, 730
BspCNI CTCAG 5 cut(s) 166, 396, 481, 562, 742
BspLI GGNNCC 5 cut(s) 102, 160, 660, 715, 756
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 2 cut(s) 59, 484
BspT107I GGYRCC 3 cut(s) 158, 658, 754
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 2 cut(s) 89, 758
BssECI CCNNGG 3 cut(s) 30, 522, 717
BssMI GATC 6 cut(s) 64, 332, 489, 538, 648, 670
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 2 cut(s) 30, 717
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 237, 732
BstDEI CTNAG 6 cut(s) 153, 404, 468, 570, 750, 777
BstF5I GGATG 2 cut(s) 382, 414
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 6 cut(s) 67, 335, 492, 541, 651, 673
BstMAI GTCTC 2 cut(s) 194, 427
BstMBI GATC 6 cut(s) 64, 332, 489, 538, 648, 670
BstMWI GCNNNNNNNGC 2 cut(s) 349, 740
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 239
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 71
BstV2I GAAGAC 2 cut(s) 389, 622
BstX2I RGATCY 3 cut(s) 332, 489, 648
BstYI RGATCY 3 cut(s) 332, 489, 648
Bsu36I CCTNAGG 1 cut(s) 750
BsuRI GGCC 2 cut(s) 5, 730
BtsCI GGATG 2 cut(s) 382, 414
BtsIMutI CAGTG 1 cut(s) 401
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 237, 732
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 3 cut(s) 456, 553, 714
Csp6I GTAC 2 cut(s) 150, 159
CviAII CATG 3 cut(s) 236, 371, 695
CviJI RGCY 9 cut(s) 5, 62, 101, 263, 368, 477, 682, 730, 776
CviKI_1 RGCY 9 cut(s) 5, 62, 101, 263, 368, 477, 682, 730, 776
CviQI GTAC 2 cut(s) 150, 159
DdeI CTNAG 6 cut(s) 153, 404, 468, 570, 750, 777
DpnI GATC 6 cut(s) 66, 334, 491, 540, 650, 672
DpnII GATC 6 cut(s) 64, 332, 489, 538, 648, 670
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 482
Eco130I CCWWGG 2 cut(s) 30, 717
Eco147I AGGCCT 1 cut(s) 730
Eco47I GGWCC 3 cut(s) 456, 553, 714
Eco57I CTGAAG 3 cut(s) 203, 285, 756
Eco81I CCTNAGG 1 cut(s) 750
EcoO109I RGGNCCY 1 cut(s) 456
EcoRII CCWGG 1 cut(s) 522
EcoT14I CCWWGG 2 cut(s) 30, 717
ErhI CCWWGG 2 cut(s) 30, 717
Esp3I CGTCTC 1 cut(s) 427
FaeI CATG 3 cut(s) 239, 374, 698
FaiI YATR 7 cut(s) 90, 237, 372, 465, 515, 686, 696
FaqI GGGAC 2 cut(s) 727, 731
FatI CATG 3 cut(s) 235, 370, 694
Fnu4HI GCNGC 2 cut(s) 60, 496
FokI GGATG 2 cut(s) 389, 421
Fsp4HI GCNGC 2 cut(s) 60, 496
FspBI CTAG 1 cut(s) 474
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 60, 496
HaeIII GGCC 2 cut(s) 5, 730
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 3 cut(s) 239, 374, 698
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 5 cut(s) 24, 136, 180, 192, 423
HphI GGTGA 4 cut(s) 136, 236, 418, 710
Hpy188I TCNGA 4 cut(s) 131, 175, 245, 291
Hpy188III TCNNGA 4 cut(s) 10, 451, 637, 646
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 8 cut(s) 34, 73, 114, 170, 227, 273, 589, 634
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 117, 436
HpyCH4V TGCA 1 cut(s) 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 349, 740
HpyF3I CTNAG 6 cut(s) 153, 404, 468, 570, 750, 777
HpySE526I ACGT 2 cut(s) 117, 436
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 3 cut(s) 239, 374, 698
HspAI GCGC 1 cut(s) 343
KpnI GGTACC 1 cut(s) 162
Kzo9I GATC 6 cut(s) 64, 332, 489, 538, 648, 670
LmnI GCTCC 3 cut(s) 106, 232, 773
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 2 cut(s) 240, 618
MaeI CTAG 1 cut(s) 474
MaeII ACGT 2 cut(s) 117, 436
MaeIII GTNAC 1 cut(s) 142
MalI GATC 6 cut(s) 66, 334, 491, 540, 650, 672
MboI GATC 6 cut(s) 64, 332, 489, 538, 648, 670
MboII GAAGA 8 cut(s) 25, 64, 103, 278, 394, 622, 716, 749
MflI RGATCY 3 cut(s) 332, 489, 648
MlyI GAGTC 3 cut(s) 130, 174, 201
MmeI TCCRAC 2 cut(s) 109, 751
MnlI CCTC 8 cut(s) 38, 223, 399, 447, 627, 682, 720, 745
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 1 cut(s) 653
MslI CAYNNNNRTG 2 cut(s) 375, 699
MspA1I CMGCKG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 524
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 349, 740
NdeII GATC 6 cut(s) 64, 332, 489, 538, 648, 670
NlaIII CATG 3 cut(s) 239, 374, 698
NlaIV GGNNCC 5 cut(s) 102, 160, 660, 715, 756
NmuCI GTSAC 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 344
NspI RCATGY 1 cut(s) 239
PaeI GCATGC 1 cut(s) 239
PceI AGGCCT 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 24, 423
PkrI GCNGC 2 cut(s) 61, 497
PleI GAGTC 3 cut(s) 130, 174, 200
PpsI GAGTC 3 cut(s) 130, 174, 200
PpuMI RGGWCCY 1 cut(s) 456
PshAI GACNNNNGTC 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 5 cut(s) 102, 160, 660, 715, 756
PspPI GGNCC 3 cut(s) 456, 553, 714
PspPPI RGGWCCY 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 3 cut(s) 332, 489, 648
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 151, 160
RsaNI GTAC 2 cut(s) 150, 159
RseI CAYNNNNRTG 2 cut(s) 375, 699
SaqAI TTAA 1 cut(s) 653
SatI GCNGC 2 cut(s) 60, 496
Sau3AI GATC 6 cut(s) 64, 332, 489, 538, 648, 670
Sau96I GGNCC 3 cut(s) 456, 553, 714
SchI GAGTC 3 cut(s) 130, 174, 201
ScrFI CCNGG 1 cut(s) 524
SfaNI GCATC 2 cut(s) 240, 618
SinI GGWCC 3 cut(s) 456, 553, 714
SmiMI CAYNNNNRTG 2 cut(s) 375, 699
SmlI CTYRAG 2 cut(s) 182, 542
SmoI CTYRAG 2 cut(s) 182, 542
SphI GCATGC 1 cut(s) 239
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 3 cut(s) 493, 496, 556
SspMI CTAG 1 cut(s) 474
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 2 cut(s) 30, 717
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 120, 439
TaqII GACCGA 1 cut(s) 521
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 2 cut(s) 24, 423
Tru1I TTAA 1 cut(s) 653
Tru9I TTAA 1 cut(s) 653
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 5 cut(s) 323, 553, 622, 683, 717
TspRI CASTG 1 cut(s) 408
VpaK11BI GGWCC 3 cut(s) 456, 553, 714
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 1 cut(s) 474
ZraI GACGTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.