MD13G1220700.v1.1

CCT motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005795
Physical Location & Seq
Reverse (-)
21352361 .. 21357007
4647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1220700.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGTTGCAGCATGTGATTCAGCAGCAGCAGCAGCAGCAGGAGCAGAATGAAATTTCCAGCCAGATTAGCTCTCAAATATTCGAATTTTGTGACCCGGAACTATTCCAAGAGACCCTACAAAATTCCGAGGTCACTTCTTCTCCAAATTGCTGCTACGAAGAAAATTCATCCTTCGTAACAAACCTTTCGCTGCCCCCAGATATAGAAACCAAGTTCAGTACCTACCAGGATAACAATGGCCATACTGACACAAGCATTTCCCAAACCCCCACAACTACTAGCACCACCACCACAACAACCACCACAAGCACCAACACCACCACCACTGCCGCCAGTGTTAATAACACCACAACCACTATCACCGATGCCATCAACAACAATAATGGCAACCTTTCCATAATATTTGATTCCCAAGAGGAAATTGATAATGATATATCAGCTTCCATCGATTTTTCTCCATCACCATCCTTTTCTGTACCTCCCTTTCTCATAACCCCGCAAGATCAATTTGATTTCTCTCAATTGCCACCTCAAATGCCATTAGCAGATTCTGTTGTTGAGGGATTTTCACAGTACCCTGACGAACCTGTACCACCTCTTATGACAGCTCCATTAGCATCAGTTTTTGAAGAGGATTGCTTGTCTTCAGTGCCTTCTTATGTTCCTTTGAACCCTTCTGCTCCTCCTTGTTCATTTCTCGGTCCTCCTATGGGACCATACATGCCTACTGGGCCTATAAATCCTGCTTCACTATCTGCTGACAACACTGGAATTTTCACCAGCAGCATTCTTATGGCTTCTGAATTGCAACAGCAAGACTTGGAATATCAAGGAGACAATGGTGGACTTTTCTGTCTAGATTCATTATCGAGGGTTTTTAACCCCGAAGAGCTTCAGGCACTTGGTGCTGAGAATCAGCAACTGGTGAGTGGGGCTGGGAACTCTACTTCTTTACCAACTGAGATTTCGATAGCCGAAGACCCAAATTTTAAAGTAGGAAAGCTCTCTGTTGAAGAGAGGAAAGAGAAGATCCACAGGTACATGAAGAAAAGGAATGAGAGGAACTTCACCAAGAAAATCAAGTATGCCTGCCGCAAAACACTTGCTGACAGCAGACCTCGAGTCCGAGGAAGATTTGCAAAGAACGATGACTTTGGAGAGACCCATCGACCTGTGGGAAGCAGTCATGAAGACGATGAAGTAGTTGTGAAAGAGGAAGAGGATTTGGTTGATTCCTCAGATATCTTTGCTCATATCAGCGGAGTGAACTCTTTCAAATGCAGCTACCCAATCCAGTCCTGGATTTGA

Protein Analysis

436

Amino Acids

47.89

Weight (kDa)

4.31

Isoelectric Point (pI)

57.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 340 - 382 9.9e-19 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 852
AciI CCGC 4 cut(s) 330, 497, 1093, 1260
AclWI GGATC 1 cut(s) 1024
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 6 cut(s) 51, 83, 121, 163, 771, 985
AcuI CTGAAG 2 cut(s) 630, 878
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 5 cut(s) 220, 477, 575, 591, 1040
AfiI CCNNNNNNNGG 1 cut(s) 710
AgsI TTSAA 4 cut(s) 629, 670, 1013, 1276
AhdI GACNNNNNGTC 1 cut(s) 1121
AjnI CCWGG 2 cut(s) 225, 1298
AjuI GAANNNNNNNTTGG 2 cut(s) 949, 981
AluBI AGCT 6 cut(s) 69, 440, 608, 892, 1003, 1284
AluI AGCT 6 cut(s) 69, 440, 608, 892, 1003, 1284
Alw26I GTCTC 3 cut(s) 104, 828, 1154
AlwI GGATC 1 cut(s) 1024
AlwNI CAGNNNCTG 2 cut(s) 551, 922
Ama87I CYCGRG 1 cut(s) 1119
AoxI GGCC 2 cut(s) 238, 731
ApoI RAATTY 6 cut(s) 51, 83, 121, 163, 771, 985
Asp700I GAANNNNTTC 2 cut(s) 891, 1271
AspS9I GGNCC 3 cut(s) 701, 713, 731
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 5 cut(s) 352, 453, 769, 937, 1060
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 1119
AvaII GGWCC 2 cut(s) 701, 713
BalI TGGCCA 1 cut(s) 240
BbsI GAAGAC 3 cut(s) 636, 984, 1197
BccI CCATC 5 cut(s) 377, 452, 466, 472, 1173
BciT130I CCWGG 2 cut(s) 227, 1300
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 3 cut(s) 104, 828, 1154
BfaI CTAG 2 cut(s) 279, 857
BglI GCCNNNNNGGC 1 cut(s) 730
Bme1390I CCNGG 3 cut(s) 95, 227, 1300
Bme18I GGWCC 2 cut(s) 701, 713
BmeRI GACNNNNNGTC 1 cut(s) 1121
BmeT110I CYCGRG 1 cut(s) 1119
BmgT120I GGNCC 3 cut(s) 701, 713, 731
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 3 cut(s) 95, 227, 1300
BmrI ACTGGG 1 cut(s) 738
BmsI GCATC 2 cut(s) 355, 626
BmuI ACTGGG 1 cut(s) 738
BpiI GAAGAC 3 cut(s) 636, 984, 1197
Bpu14I TTCGAA 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 447
BsaI GGTCTC 2 cut(s) 104, 1154
BsaJI CCNNGG 2 cut(s) 126, 1126
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 1 cut(s) 710
Bse1I ACTGG 5 cut(s) 333, 733, 772, 927, 1294
BseBI CCWGG 2 cut(s) 227, 1300
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 126, 1126
BseGI GGATG 2 cut(s) 167, 464
BseLI CCNNNNNNNGG 1 cut(s) 710
BseMII CTCAG 3 cut(s) 900, 951, 1251
BseNI ACTGG 5 cut(s) 333, 733, 772, 927, 1294
BseRI GAGGAG 1 cut(s) 672
BseYI CCCAGC 1 cut(s) 935
BshFI GGCC 2 cut(s) 240, 733
BshVI ATCGAT 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 1119
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 710
BsmAI GTCTC 3 cut(s) 104, 828, 1154
BsmFI GGGAC 1 cut(s) 726
BsmI GAATGC 1 cut(s) 786
BsnI GGCC 2 cut(s) 240, 733
Bso31I GGTCTC 2 cut(s) 104, 1154
BsoBI CYCGRG 1 cut(s) 1119
Bsp119I TTCGAA 1 cut(s) 81
Bsp143I GATC 2 cut(s) 502, 1029
BspACI CCGC 4 cut(s) 330, 497, 1093, 1260
BspANI GGCC 2 cut(s) 240, 733
BspCNI CTCAG 3 cut(s) 901, 952, 1250
BspDI ATCGAT 1 cut(s) 447
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 1 cut(s) 714
BspPI GGATC 1 cut(s) 1024
BspQI GCTCTTC 1 cut(s) 882
BspT104I TTCGAA 1 cut(s) 81
BspTNI GGTCTC 2 cut(s) 104, 1154
BsrI ACTGG 5 cut(s) 333, 733, 772, 927, 1294
BssECI CCNNGG 2 cut(s) 126, 1126
BssMI GATC 2 cut(s) 502, 1029
Bst2UI CCWGG 2 cut(s) 227, 1300
Bst4CI ACNGT 1 cut(s) 573
Bst6I CTCTTC 4 cut(s) 624, 882, 1008, 1212
BstAPI GCANNNNNTGC 1 cut(s) 905
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 1090
BstDEI CTNAG 3 cut(s) 909, 960, 1237
BstF5I GGATG 2 cut(s) 167, 464
BstKTI GATC 2 cut(s) 505, 1032
BstMAI GTCTC 3 cut(s) 104, 828, 1154
BstMBI GATC 2 cut(s) 502, 1029
BstMWI GCNNNNNNNGC 8 cut(s) 28, 31, 34, 40, 66, 614, 730, 905
BstNI CCWGG 2 cut(s) 227, 1300
BstNSI RCATGY 2 cut(s) 14, 724
BstSCI CCNGG 3 cut(s) 93, 225, 1298
BstV2I GAAGAC 3 cut(s) 636, 984, 1197
BstX2I RGATCY 1 cut(s) 1029
BstYI RGATCY 1 cut(s) 1029
Bsu15I ATCGAT 1 cut(s) 447
BsuRI GGCC 2 cut(s) 240, 733
BsuTUI ATCGAT 1 cut(s) 447
BtsCI GGATG 2 cut(s) 167, 464
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 4 cut(s) 324, 340, 654, 765
Cac8I GCNNGC 1 cut(s) 1090
CaiI CAGNNNCTG 2 cut(s) 551, 922
CciI TCATGA 1 cut(s) 1186
Cfr13I GGNCC 3 cut(s) 701, 713, 731
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 5 cut(s) 219, 476, 574, 590, 1039
CviAII CATG 4 cut(s) 11, 721, 1042, 1187
CviQI GTAC 5 cut(s) 219, 476, 574, 590, 1039
DdeI CTNAG 3 cut(s) 909, 960, 1237
DpnI GATC 2 cut(s) 504, 1031
DpnII GATC 2 cut(s) 502, 1029
DraI TTTAAA 1 cut(s) 991
DraIII CACNNNGTG 1 cut(s) 905
DrdI GACNNNNNNGTC 1 cut(s) 852
DriI GACNNNNNGTC 1 cut(s) 1121
DseDI GACNNNNNNGTC 1 cut(s) 852
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 4 cut(s) 624, 882, 1008, 1212
Eam1105I GACNNNNNGTC 1 cut(s) 1121
EarI CTCTTC 4 cut(s) 624, 882, 1008, 1212
Eco31I GGTCTC 2 cut(s) 104, 1154
Eco32I GATATC 1 cut(s) 1243
Eco47I GGWCC 2 cut(s) 701, 713
Eco57I CTGAAG 2 cut(s) 630, 878
Eco88I CYCGRG 1 cut(s) 1119
EcoRII CCWGG 2 cut(s) 225, 1298
EcoRV GATATC 1 cut(s) 1243
FaeI CATG 4 cut(s) 14, 724, 1045, 1190
FaqI GGGAC 1 cut(s) 726
FatI CATG 4 cut(s) 10, 720, 1041, 1186
FauI CCCGC 1 cut(s) 504
FokI GGATG 2 cut(s) 154, 451
FspBI CTAG 2 cut(s) 279, 857
GsaI CCCAGC 1 cut(s) 939
HaeIII GGCC 2 cut(s) 240, 733
HapII CCGG 1 cut(s) 95
Hin1II CATG 4 cut(s) 14, 724, 1045, 1190
HinfI GANTC 7 cut(s) 16, 407, 548, 860, 913, 1122, 1232
HpaII CCGG 1 cut(s) 95
HphI GGTGA 5 cut(s) 352, 453, 769, 937, 1060
Hpy166II GTNNAC 2 cut(s) 845, 1267
Hpy188I TCNGA 4 cut(s) 127, 802, 1127, 1240
Hpy188III TCNNGA 2 cut(s) 857, 1187
Hpy8I GTNNAC 2 cut(s) 845, 1267
HpyAV CCTTC 3 cut(s) 181, 663, 684
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4V TGCA 4 cut(s) 7, 808, 1139, 1281
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 31, 34, 40, 66, 614, 730, 905
HpyF3I CTNAG 3 cut(s) 909, 960, 1237
Hsp92II CATG 4 cut(s) 14, 724, 1045, 1190
Kzo9I GATC 2 cut(s) 502, 1029
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 3 cut(s) 40, 613, 685
LweI GCATC 2 cut(s) 355, 626
MaeI CTAG 2 cut(s) 279, 857
MaeIII GTNAC 3 cut(s) 89, 130, 175
MalI GATC 2 cut(s) 504, 1031
MboI GATC 2 cut(s) 502, 1029
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 1 cut(s) 1029
MlsI TGGCCA 1 cut(s) 240
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 1 cut(s) 1131
Mox20I TGGCCA 1 cut(s) 240
MroXI GAANNNNTTC 2 cut(s) 891, 1271
MscI TGGCCA 1 cut(s) 240
MseI TTAA 3 cut(s) 339, 879, 990
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 1260
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 3 cut(s) 95, 227, 1300
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 786
MvaI CCWGG 2 cut(s) 227, 1300
MwoI GCNNNNNNNGC 8 cut(s) 28, 31, 34, 40, 66, 614, 730, 905
NciI CCSGG 1 cut(s) 95
NdeII GATC 2 cut(s) 502, 1029
NlaIII CATG 4 cut(s) 14, 724, 1045, 1190
NlaIV GGNNCC 1 cut(s) 714
NmuCI GTSAC 2 cut(s) 89, 130
NspI RCATGY 2 cut(s) 14, 724
NspV TTCGAA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 1119
PagI TCATGA 1 cut(s) 1186
PciSI GCTCTTC 1 cut(s) 882
PctI GAATGC 1 cut(s) 786
PdmI GAANNNNTTC 2 cut(s) 891, 1271
PfeI GAWTC 6 cut(s) 16, 407, 548, 860, 913, 1232
PfoI TCCNGGA 1 cut(s) 1298
PleI GAGTC 1 cut(s) 1130
PpsI GAGTC 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 225, 1298
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 2 cut(s) 225, 1298
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 3 cut(s) 701, 713, 731
PspXI VCTCGAGB 1 cut(s) 1119
PstNI CAGNNNCTG 2 cut(s) 551, 922
PsuI RGATCY 1 cut(s) 1029
RsaI GTAC 5 cut(s) 220, 477, 575, 591, 1040
RsaNI GTAC 5 cut(s) 219, 476, 574, 590, 1039
RseI CAYNNNNRTG 1 cut(s) 791
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 3 cut(s) 339, 879, 990
Sau3AI GATC 2 cut(s) 502, 1029
Sau96I GGNCC 3 cut(s) 701, 713, 731
SchI GAGTC 1 cut(s) 1131
ScrFI CCNGG 3 cut(s) 95, 227, 1300
SfaNI GCATC 2 cut(s) 355, 626
Sfr274I CTCGAG 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 2 cut(s) 701, 713
SlaI CTCGAG 1 cut(s) 1119
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 1119
SmoI CTYRAG 1 cut(s) 1119
SsiI CCGC 4 cut(s) 330, 497, 1093, 1260
SspI AATATT 2 cut(s) 78, 402
SspMI CTAG 2 cut(s) 279, 857
StyD4I CCNGG 3 cut(s) 93, 225, 1298
TaaI ACNGT 1 cut(s) 573
TaqI TCGA 6 cut(s) 81, 447, 869, 968, 1120, 1168
TaqII GACCGA 1 cut(s) 689
TauI GCSGC 2 cut(s) 332, 1095
TfiI GAWTC 6 cut(s) 16, 407, 548, 860, 913, 1232
Tru1I TTAA 3 cut(s) 339, 879, 990
Tru9I TTAA 3 cut(s) 339, 879, 990
TscAI CASTG 4 cut(s) 331, 340, 654, 772
TseFI GTSAC 2 cut(s) 89, 130
Tsp45I GTSAC 2 cut(s) 89, 130
TspDTI ATGAA 7 cut(s) 63, 156, 681, 852, 1058, 1203, 1212
TspRI CASTG 4 cut(s) 331, 340, 654, 772
VpaK11BI GGWCC 2 cut(s) 701, 713
XapI RAATTY 6 cut(s) 51, 83, 121, 163, 771, 985
XbaI TCTAGA 1 cut(s) 856
XceI RCATGY 2 cut(s) 14, 724
XcmI CCANNNNNNNNNTGG 2 cut(s) 233, 1296
XhoI CTCGAG 1 cut(s) 1119
XmnI GAANNNNTTC 2 cut(s) 891, 1271
XspI CTAG 2 cut(s) 279, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.