Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002534

Physical Location & Seq

Genomic Coordinates Reverse (-)

21839676 .. 21842862

Gene Structure 3187 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1223700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGGCTTCCACGATCTTTAGAAGCCTTCACACGAACCCTTGTTTGCTCTATCGAACTGCATTGCTTAGAAGAAAAAATGCCTTTTTGTATTGCACAATGAGGAGCGCACAAACCCAGACGGCCACCACACAAGAAAAGGTTAAGTCACAAGTGAAAGTAGCACCGAATGTTCAGAACAAAACACTTGAGAAAGCCTCAAAGGACTATGAAGCTGTCATAGCTGTAGAAGCTCATGTCCAGCTCAACACTCTTACCAAGGCCTTTTGTAGTTGTTCTTATAATTATGGTTCTCCACCAAACACCAGCGTCTGCCCCATATGTATGGGTCTGCCTGGCGCTTTACCGGTTTTGAACATGAAGGTCATTGGGTCTTCAGTTAAAGTGGGACTTTCACTCAATTGCAAAATGTCATTCAACTCAACGTTTGATAGGGAACAGTACTTTTACCTGGACCTTCCGAAGGGTTACCAAATATCACAGTTTGATGTTCCAATTGCCACTGGTGGCTACATTGATGTGGATCTTCCACTAGAGTTTGGTGGTGGGCATAGAAAGTTTGGCATTACAAGAGTTCACATGGAAGAGGATGTAGGGAAGCTGCTTCATTCAGAAGGTGGACGTCACTCACAGGAAATGAACTACATTCTGGTTGATTTAAACAGAGCCGGGGTGCCCTTGCTTGAGATTGTTTCTGAAACTGATATGAGAACTGGTATTGAAGCTGCAGAATTACAGAGGTTGGTTCGGTACTTGGGAGTGAGTAATGGGAATATGCAAGAGGGTTCACTTCGTTGTGATGTGAATGTCTCTGTTCGACCAATTGGGCAAATACAGTTTGGGACAAAGTTAAGTGGAGATAAAAAATTTGAATGTTTTTCATCTGTGAGCAGGGCAATAGATTATGAAATCTCCAGGAGGTGCTTCTTCATAGTCAAGGCCAGAACAAAGAAATTGTACAGGAAACTCAAAACAGTTACAATGAGAAAGAAAGAAGGACTTGCTGACTACCGATATTTCCCAGAACCAGACCTTCCAGGAGTCTTCCTCACCCATGACTATGTTGATGCTATTCGTAATACATTGCCTGAACTTCCAGAAATGAAGCGTCGGAGATATGAAAAGATGGGTCTGAGCATGCACGATGTTCTTTTTCTTGCAAACGACGTAAACGTTGCAGAGTTTTTTGATACTACTATTACAAAGGGTGCCGATGTGAAGCTAGCTACCAACTGGATAATGAGTGATGTTGCTGCCTATATGAAAAATGAAAAATTGACCATCAATGAGATTAAGCTTAGCCCCCAAGAGTTGGCTGAGTTAATAGCTTCCATTAAAGATGGGACTATTAGTGGGAAAATAGGAAAAGAGGTAACTGATACTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006139 GO:0006399 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016070 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0034641 GO:0034660 GO:0043038 GO:0043039 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050567 GO:0070681 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00970 ko01100 map00970 map01100

Pfam Domains

Protein Families

GatB_N GatB_Yqey

Protein Analysis

462

Amino Acids

51.69

Weight (kDa)

8.88

Isoelectric Point (pI)

36.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GatB_N	PF02934	72 - 354	8.2e-90	GatB/GatE catalytic domain
GatB_Yqey	PF02637	393 - 459	8.2e-17	GatB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48520 AT1G48520 AT1G48520
fragaria_vesca	FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030 FvH4_4g05030
malus_domestica	MD13G1223700.v1.1 MD16G1228500.v1.1
prunus_persica	Prupe.1G050600_v2.0.a1
pyrus_communis	pycom13g19770 pycom16g19100
rosa_chinensis	RchiOBHm_Chr4g0395911 RchiOBHm_Chr7g0203561
rosa_laevigata	RLG00000003518 RLG00000009588
rosa_multiflora	Rmu_sc0001103.1_g000011 Rmu_sc0007757.1_g000003
rosa_roxburghii	Rroxscaffold_3G00253680 Rroxscaffold_5G00340680
rosa_rugosa	Rorug03G0353500.1 Rorug03G0353600 Rorug07G0077000
rosa_samantha	Rh4AG062900 Rh4AG063900 Rh4AG066700 Rh4BG062600 Rh4CG068900 Rh4DG058900 Rh7AG206600 Rh7BG206100 Rh7BG337200 Rh7CG219200 Rh7DG213800
rosa_wichuraiana	Rw4G005170 Rw7G018030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	279
AatII	GACGTC	1 cut(s)	622
AccB1I	GGYRCC	2 cut(s)	670, 1205
AccB7I	CCANNNNNTGG	1 cut(s)	1309
AclI	AACGTT	2 cut(s)	422, 1170
AclWI	GGATC	1 cut(s)	528
AcoI	YGGCCR	1 cut(s)	120
AcsI	RAATTY	1 cut(s)	863
AcuI	CTGAAG	1 cut(s)	357
AcyI	GRCGYC	1 cut(s)	619
AfaI	GTAC	3 cut(s)	440, 749, 956
AfiI	CCNNNNNNNGG	2 cut(s)	460, 1309
AgeI	ACCGGT	1 cut(s)	343
AgsI	TTSAA	4 cut(s)	352, 415, 719, 869
AjnI	CCWGG	4 cut(s)	331, 447, 911, 1033
Alw26I	GTCTC	1 cut(s)	811
AlwI	GGATC	1 cut(s)	528
AlwNI	CAGNNNCTG	1 cut(s)	309
AoxI	GGCC	3 cut(s)	120, 258, 936
ApeKI	GCWGC	3 cut(s)	598, 722, 1250
ApoI	RAATTY	1 cut(s)	863
AsiGI	ACCGGT	1 cut(s)	343
AspLEI	GCGC	2 cut(s)	107, 338
AspS9I	GGNCC	1 cut(s)	451
AsuC2I	CCSGG	1 cut(s)	667
AsuHPI	GGTGA	1 cut(s)	1039
AsuNHI	GCTAGC	1 cut(s)	1219
AvaII	GGWCC	1 cut(s)	451
BaeGI	GKGCMC	1 cut(s)	675
BanI	GGYRCC	2 cut(s)	670, 1205
BbsI	GAAGAC	2 cut(s)	363, 1033
BbvI	GCAGC	3 cut(s)	585, 709, 1237
BccI	CCATC	3 cut(s)	1117, 1286, 1331
BceAI	ACGGC	1 cut(s)	135
BciT130I	CCWGG	4 cut(s)	333, 449, 913, 1035
BcnI	CCSGG	1 cut(s)	667
BcoDI	GTCTC	1 cut(s)	811
BfaI	CTAG	2 cut(s)	530, 1220
BfmI	CTRYAG	2 cut(s)	222, 723
BfoI	RGCGCY	1 cut(s)	339
BisI	GCNGC	3 cut(s)	599, 723, 1251
BlpI	GCTNAGC	1 cut(s)	1295
BlsI	GCNGC	3 cut(s)	600, 724, 1252
BmcAI	AGTACT	1 cut(s)	440
Bme1390I	CCNGG	5 cut(s)	333, 449, 667, 913, 1035
Bme18I	GGWCC	1 cut(s)	451
BmgT120I	GGNCC	1 cut(s)	451
BmiI	GGNNCC	2 cut(s)	672, 1207
BmrFI	CCNGG	5 cut(s)	333, 449, 667, 913, 1035
BmsI	GCATC	1 cut(s)	1054
BmtI	GCTAGC	1 cut(s)	1223
BpiI	GAAGAC	2 cut(s)	363, 1033
BplI	GAGNNNNNCTC	4 cut(s)	179, 211, 1029, 1061
BpmI	CTGGAG	1 cut(s)	895
Bpu1102I	GCTNAGC	1 cut(s)	1295
BpuEI	CTTGAG	2 cut(s)	206, 701
BpuMI	CCSGG	1 cut(s)	667
BsaBI	GATNNNNATC	1 cut(s)	519
BsaHI	GRCGYC	1 cut(s)	619
BsaJI	CCNNGG	2 cut(s)	255, 666
BsaWI	WCCGGW	1 cut(s)	343
Bsc4I	CCNNNNNNNGG	2 cut(s)	460, 1309
Bse118I	RCCGGY	1 cut(s)	343
Bse1I	ACTGG	3 cut(s)	505, 715, 1235
Bse3DI	GCAATG	2 cut(s)	59, 1079
Bse8I	GATNNNNATC	1 cut(s)	519
BseBI	CCWGG	4 cut(s)	333, 449, 913, 1035
BseDI	CCNNGG	2 cut(s)	255, 666
BseGI	GGATG	1 cut(s)	592
BseJI	GATNNNNATC	1 cut(s)	519
BseLI	CCNNNNNNNGG	2 cut(s)	460, 1309
BseMI	GCAATG	2 cut(s)	59, 1079
BseMII	CTCAG	2 cut(s)	1121, 1305
BseNI	ACTGG	3 cut(s)	505, 715, 1235
BseRI	GAGGAG	1 cut(s)	115
BseSI	GKGCMC	1 cut(s)	675
BseXI	GCAGC	3 cut(s)	585, 709, 1237
BshFI	GGCC	3 cut(s)	122, 260, 938
BshNI	GGYRCC	2 cut(s)	670, 1205
BshTI	ACCGGT	1 cut(s)	343
BsiSI	CCGG	2 cut(s)	344, 666
BslFI	GGGAC	3 cut(s)	399, 853, 1354
BslI	CCNNNNNNNGG	2 cut(s)	460, 1309
BsmAI	GTCTC	1 cut(s)	811
BsmFI	GGGAC	3 cut(s)	399, 853, 1354
BsnI	GGCC	3 cut(s)	122, 260, 938
Bsp1286I	GDGCHC	1 cut(s)	675
Bsp1407I	TGTACA	1 cut(s)	954
Bsp143I	GATC	2 cut(s)	12, 520
Bsp1720I	GCTNAGC	1 cut(s)	1295
BspANI	GGCC	3 cut(s)	122, 260, 938
BspCNI	CTCAG	2 cut(s)	1122, 1306
BspLI	GGNNCC	2 cut(s)	672, 1207
BspMAI	CTGCAG	1 cut(s)	727
BspOI	GCTAGC	1 cut(s)	1223
BspPI	GGATC	1 cut(s)	528
BspT107I	GGYRCC	2 cut(s)	670, 1205
BsrDI	GCAATG	2 cut(s)	59, 1079
BsrFI	RCCGGY	1 cut(s)	343
BsrGI	TGTACA	1 cut(s)	954
BsrI	ACTGG	3 cut(s)	505, 715, 1235
BssAI	RCCGGY	1 cut(s)	343
BssECI	CCNNGG	2 cut(s)	255, 666
BssMI	GATC	2 cut(s)	12, 520
BssNI	GRCGYC	1 cut(s)	619
BssT1I	CCWWGG	1 cut(s)	255
Bst2UI	CCWGG	4 cut(s)	333, 449, 913, 1035
Bst4CI	ACNGT	5 cut(s)	438, 480, 834, 973, 1381
Bst6I	CTCTTC	1 cut(s)	576
BstACI	GRCGYC	1 cut(s)	619
BstAUI	TGTACA	1 cut(s)	954
BstC8I	GCNNGC	2 cut(s)	1136, 1221
BstDEI	CTNAG	4 cut(s)	65, 1130, 1295, 1314
BstEII	GGTNACC	1 cut(s)	464
BstENI	CCTNNNNNAGG	1 cut(s)	458
BstF5I	GGATG	1 cut(s)	592
BstH2I	RGCGCY	1 cut(s)	339
BstHHI	GCGC	2 cut(s)	107, 338
BstKTI	GATC	2 cut(s)	15, 523
BstMAI	GTCTC	1 cut(s)	811
BstMBI	GATC	2 cut(s)	12, 520
BstMWI	GCNNNNNNNGC	2 cut(s)	218, 227
BstNI	CCWGG	4 cut(s)	333, 449, 913, 1035
BstNSI	RCATGY	1 cut(s)	1138
BstPI	GGTNACC	1 cut(s)	464
BstSCI	CCNGG	5 cut(s)	331, 447, 665, 911, 1033
BstSFI	CTRYAG	2 cut(s)	222, 723
BstSLI	GKGCMC	1 cut(s)	675
BstV1I	GCAGC	3 cut(s)	585, 709, 1237
BstV2I	GAAGAC	2 cut(s)	363, 1033
BstX2I	RGATCY	1 cut(s)	520
BstXI	CCANNNNNNTGG	1 cut(s)	322
BstYI	RGATCY	1 cut(s)	520
BsuRI	GGCC	3 cut(s)	122, 260, 938
BtsCI	GGATG	1 cut(s)	592
BtsIMutI	CAGTG	1 cut(s)	498
Cac8I	GCNNGC	2 cut(s)	1136, 1221
CaiI	CAGNNNCTG	1 cut(s)	309
CfoI	GCGC	2 cut(s)	107, 338
Cfr10I	RCCGGY	1 cut(s)	343
Cfr13I	GGNCC	1 cut(s)	451
CseI	GACGC	2 cut(s)	295, 1094
Csp6I	GTAC	3 cut(s)	439, 748, 955
CspAI	ACCGGT	1 cut(s)	343
CviAII	CATG	5 cut(s)	233, 355, 577, 1052, 1135
CviQI	GTAC	3 cut(s)	439, 748, 955
DdeI	CTNAG	4 cut(s)	65, 1130, 1295, 1314
DpnI	GATC	2 cut(s)	14, 522
DpnII	GATC	2 cut(s)	12, 520
DraI	TTTAAA	1 cut(s)	657
EaeI	YGGCCR	1 cut(s)	120
Eam1104I	CTCTTC	1 cut(s)	576
EarI	CTCTTC	1 cut(s)	576
Eco130I	CCWWGG	1 cut(s)	255
Eco147I	AGGCCT	1 cut(s)	260
Eco47I	GGWCC	1 cut(s)	451
Eco57I	CTGAAG	1 cut(s)	357
Eco91I	GGTNACC	1 cut(s)	464
EcoNI	CCTNNNNNAGG	1 cut(s)	458
EcoO65I	GGTNACC	1 cut(s)	464
EcoRII	CCWGG	4 cut(s)	331, 447, 911, 1033
EcoT14I	CCWWGG	1 cut(s)	255
ErhI	CCWWGG	1 cut(s)	255
FaeI	CATG	5 cut(s)	236, 358, 580, 1055, 1138
FalI	AAGNNNNNCTT	4 cut(s)	372, 404, 981, 1013
FaqI	GGGAC	3 cut(s)	399, 853, 1354
FatI	CATG	5 cut(s)	232, 354, 576, 1051, 1134
FauNDI	CATATG	1 cut(s)	317
Fnu4HI	GCNGC	3 cut(s)	599, 723, 1251
FokI	GGATG	1 cut(s)	599
Fsp4HI	GCNGC	3 cut(s)	599, 723, 1251
FspBI	CTAG	2 cut(s)	530, 1220
GlaI	GCGC	2 cut(s)	106, 337
GluI	GCNGC	3 cut(s)	599, 723, 1251
GsuI	CTGGAG	1 cut(s)	895
HaeII	RGCGCY	1 cut(s)	339
HaeIII	GGCC	3 cut(s)	122, 260, 938
HapII	CCGG	2 cut(s)	344, 666
HgaI	GACGC	2 cut(s)	295, 1094
HhaI	GCGC	2 cut(s)	107, 338
Hin1I	GRCGYC	1 cut(s)	619
Hin1II	CATG	5 cut(s)	236, 358, 580, 1055, 1138
Hin6I	GCGC	2 cut(s)	105, 336
HinP1I	GCGC	2 cut(s)	105, 336
HindIII	AAGCTT	1 cut(s)	1292
HinfI	GANTC	1 cut(s)	1038
HpaII	CCGG	2 cut(s)	344, 666
HphI	GGTGA	1 cut(s)	1039
Hpy166II	GTNNAC	4 cut(s)	574, 617, 785, 1168
Hpy188I	TCNGA	6 cut(s)	174, 459, 610, 694, 1110, 1131
Hpy188III	TCNNGA	1 cut(s)	1094
Hpy8I	GTNNAC	4 cut(s)	574, 617, 785, 1168
Hpy99I	CGWCG	2 cut(s)	1110, 1166
HpyAV	CCTTC	7 cut(s)	35, 352, 454, 464, 605, 986, 1040
HpyCH4III	ACNGT	5 cut(s)	438, 480, 834, 973, 1381
HpyCH4IV	ACGT	4 cut(s)	422, 619, 1164, 1170
HpyCH4V	TGCA	8 cut(s)	59, 93, 402, 725, 775, 1138, 1157, 1175
HpyF10VI	GCNNNNNNNGC	2 cut(s)	218, 227
HpyF3I	CTNAG	4 cut(s)	65, 1130, 1295, 1314
HpySE526I	ACGT	4 cut(s)	422, 619, 1164, 1170
Hsp92I	GRCGYC	1 cut(s)	619
Hsp92II	CATG	5 cut(s)	236, 358, 580, 1055, 1138
HspAI	GCGC	2 cut(s)	105, 336
Kzo9I	GATC	2 cut(s)	12, 520
LmnI	GCTCC	1 cut(s)	102
Lsp1109I	GCAGC	3 cut(s)	585, 709, 1237
LweI	GCATC	1 cut(s)	1054
MaeI	CTAG	2 cut(s)	530, 1220
MaeII	ACGT	4 cut(s)	422, 619, 1164, 1170
MaeIII	GTNAC	5 cut(s)	144, 464, 620, 973, 1369
MalI	GATC	2 cut(s)	14, 522
MboI	GATC	2 cut(s)	12, 520
MboII	GAAGA	6 cut(s)	81, 363, 515, 593, 916, 1033
MfeI	CAATTG	3 cut(s)	397, 492, 819
MflI	RGATCY	1 cut(s)	520
MhlI	GDGCHC	1 cut(s)	675
MluCI	AATT	8 cut(s)	280, 397, 492, 728, 819, 863, 950, 1271
MlyI	GAGTC	1 cut(s)	1047
MmeI	TCCRAC	1 cut(s)	1088
MnlI	CCTC	8 cut(s)	93, 205, 577, 729, 772, 909, 1055, 1360
MseI	TTAA	7 cut(s)	141, 378, 656, 848, 1290, 1319, 1332
MslI	CAYNNNNRTG	3 cut(s)	320, 515, 1056
MspI	CCGG	2 cut(s)	344, 666
MspR9I	CCNGG	5 cut(s)	333, 449, 667, 913, 1035
MunI	CAATTG	3 cut(s)	397, 492, 819
MvaI	CCWGG	4 cut(s)	333, 449, 913, 1035
MwoI	GCNNNNNNNGC	2 cut(s)	218, 227
NciI	CCSGG	1 cut(s)	667
NdeI	CATATG	1 cut(s)	317
NdeII	GATC	2 cut(s)	12, 520
NheI	GCTAGC	1 cut(s)	1219
NlaIII	CATG	5 cut(s)	236, 358, 580, 1055, 1138
NlaIV	GGNNCC	2 cut(s)	672, 1207
NmuCI	GTSAC	2 cut(s)	144, 620
NspI	RCATGY	1 cut(s)	1138
PaeI	GCATGC	1 cut(s)	1138
PceI	AGGCCT	1 cut(s)	260
PcsI	WCGNNNNNNNCGW	1 cut(s)	1167
PflMI	CCANNNNNTGG	1 cut(s)	1309
PfoI	TCCNGGA	2 cut(s)	911, 1033
PinAI	ACCGGT	1 cut(s)	343
PkrI	GCNGC	3 cut(s)	600, 724, 1252
PleI	GAGTC	1 cut(s)	1046
PpsI	GAGTC	1 cut(s)	1046
PsiI	TTATAA	1 cut(s)	279
Psp1406I	AACGTT	2 cut(s)	422, 1170
Psp6I	CCWGG	4 cut(s)	331, 447, 911, 1033
PspEI	GGTNACC	1 cut(s)	464
PspGI	CCWGG	4 cut(s)	331, 447, 911, 1033
PspN4I	GGNNCC	2 cut(s)	672, 1207
PspPI	GGNCC	1 cut(s)	451
PstI	CTGCAG	1 cut(s)	727
PstNI	CAGNNNCTG	1 cut(s)	309
PsuI	RGATCY	1 cut(s)	520
RsaI	GTAC	3 cut(s)	440, 749, 956
RsaNI	GTAC	3 cut(s)	439, 748, 955
RseI	CAYNNNNRTG	3 cut(s)	320, 515, 1056
SaqAI	TTAA	7 cut(s)	141, 378, 656, 848, 1290, 1319, 1332
SatI	GCNGC	3 cut(s)	599, 723, 1251
Sau3AI	GATC	2 cut(s)	12, 520
Sau96I	GGNCC	1 cut(s)	451
ScaI	AGTACT	1 cut(s)	440
SchI	GAGTC	1 cut(s)	1047
ScrFI	CCNGG	5 cut(s)	333, 449, 667, 913, 1035
SduI	GDGCHC	1 cut(s)	675
SfaNI	GCATC	1 cut(s)	1054
SfcI	CTRYAG	2 cut(s)	222, 723
SinI	GGWCC	1 cut(s)	451
SmiMI	CAYNNNNRTG	3 cut(s)	320, 515, 1056
SmlI	CTYRAG	2 cut(s)	185, 680
SmoI	CTYRAG	2 cut(s)	185, 680
SphI	GCATGC	1 cut(s)	1138
Sse9I	AATT	8 cut(s)	280, 397, 492, 728, 819, 863, 950, 1271
SseBI	AGGCCT	1 cut(s)	260
SspMI	CTAG	2 cut(s)	530, 1220
StuI	AGGCCT	1 cut(s)	260
StyD4I	CCNGG	5 cut(s)	331, 447, 665, 911, 1033
StyI	CCWWGG	1 cut(s)	255
TaaI	ACNGT	5 cut(s)	438, 480, 834, 973, 1381
TaiI	ACGT	4 cut(s)	425, 622, 1167, 1173
TaqI	TCGA	2 cut(s)	52, 814
TasI	AATT	8 cut(s)	280, 397, 492, 728, 819, 863, 950, 1271
TatI	WGTACW	2 cut(s)	438, 954
Tru1I	TTAA	7 cut(s)	141, 378, 656, 848, 1290, 1319, 1332
Tru9I	TTAA	7 cut(s)	141, 378, 656, 848, 1290, 1319, 1332
TscAI	CASTG	1 cut(s)	505
TseFI	GTSAC	2 cut(s)	144, 620
TseI	GCWGC	3 cut(s)	598, 722, 1250
Tsp45I	GTSAC	2 cut(s)	144, 620
TspRI	CASTG	1 cut(s)	505
Van91I	CCANNNNNTGG	1 cut(s)	1309
VpaK11BI	GGWCC	1 cut(s)	451
XagI	CCTNNNNNAGG	1 cut(s)	458
XapI	RAATTY	1 cut(s)	863
XceI	RCATGY	1 cut(s)	1138
XspI	CTAG	2 cut(s)	530, 1220
ZraI	GACGTC	1 cut(s)	620
ZrmI	AGTACT	1 cut(s)	440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.