MD13G1243700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008661
Physical Location & Seq
Forward (+)
25407403 .. 25411398
3996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1243700.v1.1.491

Sequence Viewer

Length: 3234 bp
ATGATAATCTTCTTCTCCTCAATCTTCCACACCAAACTAATCCGACCCTTCTCTTCTTCTTCATCTCCTTGCCGAACCAACCTCCCGGAACCCCCACCTCTTCAGACTCTGCTCAAAAGCGGCTTCTCTCCCACTCTCAAATCCATCGTCCAATTCCTTCTCTTCCTCTCTCGAACCCGCCGGTTCGACACCCTCGTCCACTTCTTCTCCCAAATGGAGTCCAACCAAATCAAAGGCAGCGCCCAAACGCACGTAATTCTCACCTGGGCTCTCCTAAATTTGCAGAAATATGAAGAAGCAGAGCATTTTATGAGGACCCGGATGGTCGAAGCTTCGAGTTTGCGGCGGAATCGGATGTGGGACTCTCTGATTCAAGGCCTATGTGTCAACCGGAAAGACCCCGAGAAGGCTTTGTTGGTGTTGCGGGATTGCTTGGGGAGCTACGGTATTTTTCCTTCTTCTTTCACTTTTTGCTCGTTAATTCGTTCGTTTAGCAATCAGGGGGATATGAGCAAGGCAATTGAGGTGCTGGAATTGATGACTGATGAGAAAATTGGGAAGCCGGAAATCGCAGTCAAGTTTTTCAAGAATGCTGTGGCTTCGGGAGCTTTAGAGCCTAATGTTGTGACTTATACAGCACTTGCAGGTGCTCTTTGTAAATTGGGTAGAGTTAATGAGGTTTGTGATTTGGTTTGTAGGGTTGAGAAGAGAGGTTGGGCATTTGATGTTGTTTTCTTCAGTATTTGGATTTGTGGTTATATTTCGGAAGGGGTTCTAATGGAGGTATTTCGGAAGAATAGAAAGATGGTGAACAAAGGCATAAGACCTGATACAATTAGCCATAGTATTATGATAGATGGGTTTTCCAAGCTAGGAGATGTGGAAAAAGCGCTCGGTATTGTAATAAAGATGAGGAAAGATGGGCTTGAACCCAATTTGATCACGTATACTGCCATCCTGTTGGGGTTTTGCAAGAAAGGGAAAATGGAGGAGGCATTTGCTATTTTCAAGATGGTTGAAGATTTGGGAATTGTAGTTGATGAATTCATGTATGCAACTTTAATTCACGGGTCCTGCATGAGAGGAGATCTCGATGATGTTTTTGATTTGCTACATAAAATGGAGGAGAGGGGGATTAATCCAAGCATTGTCACATACAATACTGTAATTAATGGATTATGCAAATTTGGGAGGACATGCGAGGCTGATGAGATCTCAAAGGGTATACTTGGAGATACAATTACATATAGTACGCTGCTACATGGATATATTGAGGAAGAAAATACCACGGGGATTCTTGAAACGAAGAGAAGATTGGAGGAAGCCGGAGTCTACATGGATGTTGTTATGTGTAACATACTTATCAAAGCACTGTTCATGGTGGGGGCATTTGACGATGCTTATATACTATACAAGGGAATGCCAGATAAGGGTTTGGTTGCAGATTCCAGTACATATTGTACAATGATTGACGGGTACTGTAAAGCTGGTAGAATGGATGGGGCACTTGAGATATTTGATGAGTTCAGAAGGACACTAGGTTCCTCCGTTGCATGTTATAATTGTATTATCAGTTCGCTCTGTAAGCAGGGAATGGTAGATATGGCCGCAGAGGTATTTATTGAACTCAGTGGGAAAGGTTTAGGTTTGGATGTAGGTATCTATAATATTCTGTTGAAGGCAATTTTTGAAGACAAAAGTGCAGTTGGAGTTATAAATTTGGTTCGACGGATTGATAGTTTGAAGACAGAAGTGTATGATATTGTTTGCAATGATGCTATCAGCTTCTTGTGCAGGAGAGGTTTTCCTGAGTCTGCATGTGAAGTGTATTTGGTGATGAGGAGGAAAGGGTCAGTTGCCACAAGCAAAACTTACTGTTCAATTTTAGAAGGTCTTATCAGTGATGGGAAAGAGTGGCTAACTCAATCCTTCTTGACCATCTTTGTGAAAGAATATGGCCTAGAAGAACCCACGGTAAGCAAGATTCTAGCTTACTACATAAGTCTGAAGAATGTTGATGATGCTTTCTGGTTTCTACACAAAATGAAGGATAAGCCTGCAGCCGTCACTTTGCCTGTTAGTCTTTATAAGACACTTATAAAGACTGGTAGAGTTTTGGATGCATATAAACTTGTCATGGTGGCCGGAGATGGTCTACCTATCCTGGATGCATTTGATTATTCACTTATGGTTGATAGTCTTTGTAAACGAGGACATATCAGTGAGGCATTAGATTTGTGTAGTATTGCTAAAAATAAAGGAGTGGCCCTTGATATCATCACTTATAATTCAGTTATAAATGCATTATGCCGCCAAGGCCACCTTGTTGAAGCATTTCGGCTATTTGATTCATTAGAGAGAATTAATTTGGTACCCACGGAAATCACATATGCTACATTGATTGATGCCTTACGTAGACAGGGTTTTCTTTTGGATGCCAAGGAGTTATTTGAGAGGATGGTTCTCAAGGGCTTTAAGCCAAATACACATGTTTACAACTTGATTATTGATGGTTATTGCAAAATTGGAGATATGGATGATGCCTTGAAGCTTCTTTATGAATTGGATTTAAAATCCCTCAGGCCTGATGAATTCACTGTTAGTATTATAATCAATGGCTTTTGTCTAAAGGGTGATATGGAAGGAGCTCTTGAATTCTTTGTTGAGCTCAAGGAAAAAGGTACATTACCTGATTTTTTGGGTTTCTTATATTTACTAAGAGGTCTATGCGCTAAGGGAAGGATGGAAGAAGCCAGAACCATCTTGAGAGAAATGCTCAACTCCCAGTCTGTTTTGGAGCTAATAAATAGAGTTGATGTTGAGGTTGAAACAGATTCATTAGAAGGTTTTCTTGCATCTTTATGTGAGCAAGGCCGTATCGAAGAATCACTTACTGTCCTTAATGAAATTGGGTGCATGTTTTTCCCTGTTAGAGGTTCACCTAATAATCACCAACAATTTCTTAAATTAGACAAGCCTTATGACAGGGAGCCTTCTGGTATAGTTGTTTCAAATTCTGTAACCTCCACCGGTGCAGATTTGGATATTCAGCTTTGTGAAATGAAGAAAGTAGAGAAGGTGGCGGAAAATTATGACGGTGGAGGGAGATGGTCTCAGTTTAAAGATTTTGACGATTGCTATAAGCAAGTTGCCACACTCTGTTCATGTGGGGAGATACAAAAGGCTAGTCAATTAGCAAAGGAGATGGTTTCTAATTTTAGAAGGGCCAGTTAG

Protein Analysis

1078

Amino Acids

120.86

Weight (kDa)

5.69

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S27 PF10037 107 - 188 3.1e-07 Mitochondrial 28S ribosomal protein S27
PPR_2 PF13041 242 - 290 1.2e-06 PPR repeat family
PPR_long PF17177 259 - 387 2e-13 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 267 - 320 2e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 284 - 325 2.8e-09 PPR repeat family
PPR_1 PF12854 307 - 337 9.3e-09 PPR repeat
PPR_2 PF13041 312 - 359 1.6e-08 PPR repeat family
TPR_24 PF23276 326 - 472 3.8e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 335 - 391 7.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 343 - 375 4.8e-06 PPR repeat
PPR_2 PF13041 347 - 395 2.8e-14 PPR repeat family
PPR_1 PF12854 377 - 402 1.3e-07 PPR repeat
PPR_2 PF13041 447 - 495 6.7e-12 PPR repeat family
PPR_1 PF12854 477 - 508 1e-10 PPR repeat
PPR PF01535 484 - 510 5e-08 PPR repeat
PPR_2 PF13041 516 - 561 1.4e-07 PPR repeat family
PPR_2 PF13041 727 - 770 3.5e-08 PPR repeat family
PPR_long PF17177 727 - 871 5.1e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 744 - 851 9e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 750 - 803 2.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 753 - 784 2.8e-10 PPR repeat
PPR_2 PF13041 758 - 804 5.5e-13 PPR repeat family
PPR PF01535 760 - 788 2.8e-06 PPR repeat
PPR_1 PF12854 789 - 821 9.6e-06 PPR repeat
PPR_1 PF12854 823 - 852 1.6e-09 PPR repeat
PPR_2 PF13041 827 - 875 3.6e-14 PPR repeat family
PPR_1 PF12854 859 - 889 2.2e-07 PPR repeat
PPR_2 PF13041 869 - 910 4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 7 cut(s) 1560, 1715, 2088, 2099, 2286, 2297, 2609
AarI CACCTGC 1 cut(s) 635
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 2371
AccB1I GGYRCC 1 cut(s) 2371
AccI GTMKAC 5 cut(s) 947, 1225, 1332, 2155, 2416
AciI CCGC 8 cut(s) 120, 178, 343, 346, 424, 1608, 2311, 3083
AcoI YGGCCR 2 cut(s) 1605, 2142
AcsI RAATTY 7 cut(s) 277, 1043, 1184, 1717, 2591, 2654, 3013
AcuI CTGAAG 3 cut(s) 86, 721, 2027
AfaI GTAC 6 cut(s) 1252, 1453, 1462, 1478, 2373, 2683
AfeI AGCGCT 1 cut(s) 891
AfiI CCNNNNNNNGG 3 cut(s) 406, 1430, 2933
AflIII ACRYGT 1 cut(s) 2488
AgeI ACCGGT 1 cut(s) 3029
AjnI CCWGG 2 cut(s) 263, 2163
AjuI GAANNNNNNNTTGG 4 cut(s) 398, 430, 1298, 1330
AloI GAACNNNNNNTCC 2 cut(s) 1525, 1557
Alw21I GWGCWC 3 cut(s) 652, 2650, 2670
Alw26I GTCTC 1 cut(s) 3117
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 401
Aor51HI AGCGCT 1 cut(s) 891
AoxI GGCC 9 cut(s) 376, 1605, 1957, 2142, 2265, 2317, 2582, 2872, 3225
ApeKI GCWGC 3 cut(s) 237, 1255, 2060
ApoI RAATTY 7 cut(s) 277, 1043, 1184, 1717, 2591, 2654, 3013
ArsI GACNNNNNNTTYG 4 cut(s) 132, 164, 3172, 3204
AseI ATTAAT 3 cut(s) 1137, 1170, 2364
AsiGI ACCGGT 1 cut(s) 3029
Asp700I GAANNNNTTC 3 cut(s) 771, 2334, 2847
Asp718I GGTACC 1 cut(s) 2371
AspLEI GCGC 3 cut(s) 242, 892, 2732
AspS9I GGNCC 4 cut(s) 315, 1071, 2266, 3225
AsuC2I CCSGG 2 cut(s) 86, 319
AsuHPI GGTGA 6 cut(s) 253, 820, 1846, 2645, 2931, 2942
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 2 cut(s) 315, 1071
AxyI CCTNAGG 1 cut(s) 2579
BaeGI GKGCMC 1 cut(s) 1507
BanI GGYRCC 1 cut(s) 2371
BanII GRGCYC 3 cut(s) 271, 2650, 2670
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BbsI GAAGAC 2 cut(s) 1698, 1751
Bbv12I GWGCWC 3 cut(s) 652, 2650, 2670
BbvI GCAGC 3 cut(s) 249, 1242, 2072
BceAI ACGGC 2 cut(s) 2048, 2859
BciT130I CCWGG 2 cut(s) 265, 2165
BclI TGATCA 1 cut(s) 939
BcnI CCSGG 2 cut(s) 86, 319
BcoDI GTCTC 1 cut(s) 3117
BfaI CTAG 5 cut(s) 872, 1538, 1961, 1988, 3186
BfmI CTRYAG 1 cut(s) 2058
BfoI RGCGCY 2 cut(s) 243, 893
BfuAI ACCTGC 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 2316
BglII AGATCT 2 cut(s) 1087, 1212
BisI GCNGC 7 cut(s) 121, 238, 344, 1256, 1608, 2061, 2311
BlsI GCNGC 7 cut(s) 122, 239, 345, 1257, 1609, 2062, 2312
Bme1390I CCNGG 4 cut(s) 86, 265, 319, 2165
Bme18I GGWCC 2 cut(s) 315, 1071
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 4 cut(s) 315, 1071, 2266, 3225
BmiI GGNNCC 6 cut(s) 90, 317, 1072, 1543, 2373, 2991
BmrFI CCNGG 4 cut(s) 86, 265, 319, 2165
BmrI ACTGGG 1 cut(s) 2779
BmsI GCATC 9 cut(s) 1387, 1765, 2011, 2110, 2158, 2395, 2425, 2530, 2864
BmuI ACTGGG 1 cut(s) 2779
BpiI GAAGAC 2 cut(s) 1698, 1751
BplI GAGNNNNNCTC 6 cut(s) 1074, 1106, 2760, 2792, 3097, 3129
Bpu10I CCTNAGC 1 cut(s) 2733
BpuEI CTTGAG 4 cut(s) 1529, 2450, 2654, 2785
BpuMI CCSGG 2 cut(s) 86, 319
BsaAI YACGTR 3 cut(s) 253, 945, 2414
BsaI GGTCTC 1 cut(s) 3117
BsaJI CCNNGG 6 cut(s) 264, 1287, 1971, 2314, 2376, 2439
BsaWI WCCGGW 2 cut(s) 390, 3029
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 430, 460
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 1430, 2933
Bse118I RCCGGY 2 cut(s) 180, 3029
Bse1I ACTGG 4 cut(s) 1449, 2110, 2785, 3228
Bse21I CCTNAGG 1 cut(s) 2579
Bse3DI GCAATG 1 cut(s) 1777
BseBI CCWGG 2 cut(s) 265, 2165
BseDI CCNNGG 6 cut(s) 264, 1287, 1971, 2314, 2376, 2439
BseLI CCNNNNNNNGG 3 cut(s) 406, 1430, 2933
BseMI GCAATG 1 cut(s) 1777
BseMII CTCAG 4 cut(s) 1642, 1800, 2593, 3128
BseNI ACTGG 4 cut(s) 1449, 2110, 2785, 3228
BseRI GAGGAG 5 cut(s) 7, 1004, 1098, 1139, 1855
BseSI GKGCMC 1 cut(s) 1507
BseXI GCAGC 3 cut(s) 249, 1242, 2072
BsgI GTGCAG 3 cut(s) 1722, 1813, 3054
BshFI GGCC 9 cut(s) 378, 1607, 1959, 2144, 2267, 2319, 2584, 2874, 3227
BshNI GGYRCC 1 cut(s) 2371
BshTI ACCGGT 1 cut(s) 3029
BsiHKAI GWGCWC 3 cut(s) 652, 2650, 2670
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 8 cut(s) 86, 181, 319, 391, 563, 1326, 2145, 3030
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 3 cut(s) 406, 1430, 2933
BsmAI GTCTC 1 cut(s) 3117
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 2 cut(s) 595, 1425
BsnI GGCC 9 cut(s) 378, 1607, 1959, 2144, 2267, 2319, 2584, 2874, 3227
Bso31I GGTCTC 1 cut(s) 3117
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 5 cut(s) 271, 652, 1507, 2650, 2670
Bsp1407I TGTACA 1 cut(s) 1460
Bsp143I GATC 3 cut(s) 939, 1087, 1212
BspACI CCGC 8 cut(s) 120, 178, 343, 346, 424, 1608, 2311, 3083
BspANI GGCC 9 cut(s) 378, 1607, 1959, 2144, 2267, 2319, 2584, 2874, 3227
BspCNI CTCAG 4 cut(s) 1641, 1801, 2592, 3127
BspLI GGNNCC 6 cut(s) 90, 317, 1072, 1543, 2373, 2991
BspMAI CTGCAG 1 cut(s) 2062
BspMI ACCTGC 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 2371
BspTNI GGTCTC 1 cut(s) 3117
BsrDI GCAATG 1 cut(s) 1777
BsrFI RCCGGY 2 cut(s) 180, 3029
BsrGI TGTACA 1 cut(s) 1460
BsrI ACTGG 4 cut(s) 1449, 2110, 2785, 3228
BssAI RCCGGY 2 cut(s) 180, 3029
BssECI CCNNGG 6 cut(s) 264, 1287, 1971, 2314, 2376, 2439
BssMI GATC 3 cut(s) 939, 1087, 1212
BssNAI GTATAC 2 cut(s) 948, 1226
BssT1I CCWWGG 2 cut(s) 2314, 2439
Bst1107I GTATAC 2 cut(s) 948, 1226
Bst2UI CCWGG 2 cut(s) 265, 2165
Bst4CI ACNGT 9 cut(s) 446, 1165, 1374, 1481, 1877, 1975, 2599, 2896, 3098
Bst6I CTCTTC 5 cut(s) 58, 105, 167, 701, 1301
BstAUI TGTACA 1 cut(s) 1460
BstBAI YACGTR 3 cut(s) 253, 945, 2414
BstC8I GCNNGC 1 cut(s) 2058
BstDEI CTNAG 6 cut(s) 1628, 1809, 2579, 2717, 2733, 3114
BstDSI CCRYGG 3 cut(s) 1287, 1971, 2376
BstENI CCTNNNNNAGG 1 cut(s) 2931
BstH2I RGCGCY 2 cut(s) 243, 893
BstHHI GCGC 3 cut(s) 242, 892, 2732
BstKTI GATC 3 cut(s) 942, 1090, 1215
BstMAI GTCTC 1 cut(s) 3117
BstMBI GATC 3 cut(s) 939, 1087, 1212
BstMWI GCNNNNNNNGC 5 cut(s) 438, 605, 1585, 1791, 2316
BstNI CCWGG 2 cut(s) 265, 2165
BstNSI RCATGY 5 cut(s) 1200, 1557, 1821, 2492, 2920
BstSCI CCNGG 4 cut(s) 84, 263, 317, 2163
BstSFI CTRYAG 1 cut(s) 2058
BstSLI GKGCMC 1 cut(s) 1507
BstSNI TACGTA 1 cut(s) 2414
BstV1I GCAGC 3 cut(s) 249, 1242, 2072
BstV2I GAAGAC 2 cut(s) 1698, 1751
BstX2I RGATCY 2 cut(s) 1087, 1212
BstXI CCANNNNNNTGG 1 cut(s) 961
BstYI RGATCY 2 cut(s) 1087, 1212
BstZ17I GTATAC 2 cut(s) 948, 1226
Bsu36I CCTNAGG 1 cut(s) 2579
BsuRI GGCC 9 cut(s) 378, 1607, 1959, 2144, 2267, 2319, 2584, 2874, 3227
BtgI CCRYGG 3 cut(s) 1287, 1971, 2376
BtsIMutI CAGTG 5 cut(s) 1370, 1636, 1906, 2227, 2595
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 2058
CaiI CAGNNNCTG 1 cut(s) 109
CfoI GCGC 3 cut(s) 242, 892, 2732
Cfr10I RCCGGY 2 cut(s) 180, 3029
Cfr13I GGNCC 4 cut(s) 315, 1071, 2266, 3225
Csp6I GTAC 6 cut(s) 1251, 1452, 1461, 1477, 2372, 2682
CspAI ACCGGT 1 cut(s) 3029
CviQI GTAC 6 cut(s) 1251, 1452, 1461, 1477, 2372, 2682
DdeI CTNAG 6 cut(s) 1628, 1809, 2579, 2717, 2733, 3114
DpnI GATC 3 cut(s) 941, 1089, 1214
DpnII GATC 3 cut(s) 939, 1087, 1212
DraI TTTAAA 2 cut(s) 2571, 3121
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 2 cut(s) 1605, 2142
Eam1104I CTCTTC 5 cut(s) 58, 105, 167, 701, 1301
EarI CTCTTC 5 cut(s) 58, 105, 167, 701, 1301
EciI GGCGGA 2 cut(s) 361, 3098
Ecl136II GAGCTC 2 cut(s) 2648, 2668
Eco105I TACGTA 1 cut(s) 2414
Eco130I CCWWGG 2 cut(s) 2314, 2439
Eco147I AGGCCT 2 cut(s) 378, 2584
Eco24I GRGCYC 3 cut(s) 271, 2650, 2670
Eco31I GGTCTC 1 cut(s) 3117
Eco32I GATATC 1 cut(s) 2275
Eco47I GGWCC 2 cut(s) 315, 1071
Eco47III AGCGCT 1 cut(s) 891
Eco53kI GAGCTC 2 cut(s) 2648, 2668
Eco57I CTGAAG 3 cut(s) 86, 721, 2027
Eco81I CCTNAGG 1 cut(s) 2579
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 2648, 2668
EcoNI CCTNNNNNAGG 1 cut(s) 2931
EcoO109I RGGNCCY 2 cut(s) 315, 1071
EcoRI GAATTC 3 cut(s) 1043, 2591, 2654
EcoRII CCWGG 2 cut(s) 263, 2163
EcoRV GATATC 1 cut(s) 2275
EcoT14I CCWWGG 2 cut(s) 2314, 2439
EcoT22I ATGCAT 3 cut(s) 2125, 2173, 2305
EcoT38I GRGCYC 3 cut(s) 271, 2650, 2670
ErhI CCWWGG 2 cut(s) 2314, 2439
FaqI GGGAC 1 cut(s) 374
FauI CCCGC 2 cut(s) 185, 417
FauNDI CATATG 1 cut(s) 2389
FbaI TGATCA 1 cut(s) 939
FblI GTMKAC 5 cut(s) 947, 1225, 1332, 2155, 2416
Fnu4HI GCNGC 7 cut(s) 121, 238, 344, 1256, 1608, 2061, 2311
FriOI GRGCYC 3 cut(s) 271, 2650, 2670
Fsp4HI GCNGC 7 cut(s) 121, 238, 344, 1256, 1608, 2061, 2311
FspBI CTAG 5 cut(s) 872, 1538, 1961, 1988, 3186
GlaI GCGC 3 cut(s) 241, 891, 2731
GluI GCNGC 7 cut(s) 121, 238, 344, 1256, 1608, 2061, 2311
HaeII RGCGCY 2 cut(s) 243, 893
HaeIII GGCC 9 cut(s) 378, 1607, 1959, 2144, 2267, 2319, 2584, 2874, 3227
HapII CCGG 8 cut(s) 86, 181, 319, 391, 563, 1326, 2145, 3030
HhaI GCGC 3 cut(s) 242, 892, 2732
Hin6I GCGC 3 cut(s) 240, 890, 2730
HinP1I GCGC 3 cut(s) 240, 890, 2730
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 2 cut(s) 330, 2549
HpaII CCGG 8 cut(s) 86, 181, 319, 391, 563, 1326, 2145, 3030
HphI GGTGA 6 cut(s) 253, 820, 1846, 2645, 2931, 2942
Hpy188I TCNGA 8 cut(s) 44, 105, 354, 369, 766, 792, 1529, 2007
Hpy99I CGWCG 1 cut(s) 1731
HpyCH4III ACNGT 9 cut(s) 446, 1165, 1374, 1481, 1877, 1975, 2599, 2896, 3098
HpyCH4IV ACGT 3 cut(s) 252, 944, 2413
HpyF10VI GCNNNNNNNGC 5 cut(s) 438, 605, 1585, 1791, 2316
HpyF3I CTNAG 6 cut(s) 1628, 1809, 2579, 2717, 2733, 3114
HpySE526I ACGT 3 cut(s) 252, 944, 2413
HspAI GCGC 3 cut(s) 240, 890, 2730
KpnI GGTACC 1 cut(s) 2375
Ksp22I TGATCA 1 cut(s) 939
Kzo9I GATC 3 cut(s) 939, 1087, 1212
LmnI GCTCC 5 cut(s) 438, 605, 2645, 2797, 2989
Lsp1109I GCAGC 3 cut(s) 249, 1242, 2072
LweI GCATC 9 cut(s) 1387, 1765, 2011, 2110, 2158, 2395, 2425, 2530, 2864
MaeI CTAG 5 cut(s) 872, 1538, 1961, 1988, 3186
MaeII ACGT 3 cut(s) 252, 944, 2413
MaeIII GTNAC 5 cut(s) 625, 1150, 1352, 2065, 3019
MalI GATC 3 cut(s) 941, 1089, 1214
MboI GATC 3 cut(s) 939, 1087, 1212
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 2 cut(s) 1087, 1212
MhlI GDGCHC 5 cut(s) 271, 652, 1507, 2650, 2670
MlyI GAGTC 5 cut(s) 100, 227, 356, 1338, 1820
MmeI TCCRAC 3 cut(s) 67, 246, 1687
Mph1103I ATGCAT 3 cut(s) 2125, 2173, 2305
MroXI GAANNNNTTC 3 cut(s) 771, 2334, 2847
MslI CAYNNNNRTG 3 cut(s) 1943, 2220, 2860
MspI CCGG 8 cut(s) 86, 181, 319, 391, 563, 1326, 2145, 3030
MspR9I CCNGG 4 cut(s) 86, 265, 319, 2165
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 2 cut(s) 595, 1425
MvaI CCWGG 2 cut(s) 265, 2165
MwoI GCNNNNNNNGC 5 cut(s) 438, 605, 1585, 1791, 2316
NciI CCSGG 2 cut(s) 86, 319
NdeI CATATG 1 cut(s) 2389
NdeII GATC 3 cut(s) 939, 1087, 1212
NlaIV GGNNCC 6 cut(s) 90, 317, 1072, 1543, 2373, 2991
NmuCI GTSAC 3 cut(s) 625, 1150, 2065
NsiI ATGCAT 3 cut(s) 2125, 2173, 2305
NspI RCATGY 5 cut(s) 1200, 1557, 1821, 2492, 2920
PaqCI CACCTGC 1 cut(s) 635
PceI AGGCCT 2 cut(s) 378, 2584
PciI ACATGT 1 cut(s) 2488
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 2 cut(s) 595, 1425
PdmI GAANNNNTTC 3 cut(s) 771, 2334, 2847
PfeI GAWTC 8 cut(s) 349, 370, 1294, 1445, 1984, 2348, 2834, 2885
PfoI TCCNGGA 2 cut(s) 84, 2163
PinAI ACCGGT 1 cut(s) 3029
PkrI GCNGC 7 cut(s) 122, 239, 345, 1257, 1609, 2062, 2312
PleI GAGTC 5 cut(s) 100, 226, 356, 1337, 1819
PpsI GAGTC 5 cut(s) 100, 226, 356, 1337, 1819
Ppu21I YACGTR 3 cut(s) 253, 945, 2414
PpuMI RGGWCCY 2 cut(s) 315, 1071
PscI ACATGT 1 cut(s) 2488
PshBI ATTAAT 3 cut(s) 1137, 1170, 2364
PsiI TTATAA 7 cut(s) 1560, 1715, 2088, 2099, 2286, 2297, 2609
Psp124BI GAGCTC 2 cut(s) 2650, 2670
Psp5II RGGWCCY 2 cut(s) 315, 1071
Psp6I CCWGG 2 cut(s) 263, 2163
PspGI CCWGG 2 cut(s) 263, 2163
PspN4I GGNNCC 6 cut(s) 90, 317, 1072, 1543, 2373, 2991
PspPI GGNCC 4 cut(s) 315, 1071, 2266, 3225
PspPPI RGGWCCY 2 cut(s) 315, 1071
PsrI GAACNNNNNNTAC 2 cut(s) 1558, 1590
PstI CTGCAG 1 cut(s) 2062
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 2 cut(s) 1087, 1212
RsaI GTAC 6 cut(s) 1252, 1453, 1462, 1478, 2373, 2683
RsaNI GTAC 6 cut(s) 1251, 1452, 1461, 1477, 2372, 2682
RseI CAYNNNNRTG 3 cut(s) 1943, 2220, 2860
SacI GAGCTC 2 cut(s) 2650, 2670
SatI GCNGC 7 cut(s) 121, 238, 344, 1256, 1608, 2061, 2311
Sau3AI GATC 3 cut(s) 939, 1087, 1212
Sau96I GGNCC 4 cut(s) 315, 1071, 2266, 3225
SchI GAGTC 5 cut(s) 100, 227, 356, 1338, 1820
ScrFI CCNGG 4 cut(s) 86, 265, 319, 2165
SduI GDGCHC 5 cut(s) 271, 652, 1507, 2650, 2670
SfaNI GCATC 9 cut(s) 1387, 1765, 2011, 2110, 2158, 2395, 2425, 2530, 2864
SfcI CTRYAG 1 cut(s) 2058
SgrAI CRCCGGYG 1 cut(s) 3029
SinI GGWCC 2 cut(s) 315, 1071
SmiMI CAYNNNNRTG 3 cut(s) 1943, 2220, 2860
SmlI CTYRAG 4 cut(s) 1508, 2465, 2669, 2764
SmoI CTYRAG 4 cut(s) 1508, 2465, 2669, 2764
SnaBI TACGTA 1 cut(s) 2414
SseBI AGGCCT 2 cut(s) 378, 2584
SsiI CCGC 8 cut(s) 120, 178, 343, 346, 424, 1608, 2311, 3083
SspI AATATT 1 cut(s) 1669
SspMI CTAG 5 cut(s) 872, 1538, 1961, 1988, 3186
SstI GAGCTC 2 cut(s) 2650, 2670
StuI AGGCCT 2 cut(s) 378, 2584
StyD4I CCNGG 4 cut(s) 84, 263, 317, 2163
StyI CCWWGG 2 cut(s) 2314, 2439
TaaI ACNGT 9 cut(s) 446, 1165, 1374, 1481, 1877, 1975, 2599, 2896, 3098
TaiI ACGT 3 cut(s) 255, 947, 2416
TaqI TCGA 7 cut(s) 172, 186, 327, 335, 1092, 1726, 2880
TatI WGTACW 2 cut(s) 1451, 1460
TauI GCSGC 4 cut(s) 123, 346, 1610, 2313
TfiI GAWTC 8 cut(s) 349, 370, 1294, 1445, 1984, 2348, 2834, 2885
TscAI CASTG 5 cut(s) 1377, 1636, 1906, 2227, 2602
TseFI GTSAC 3 cut(s) 625, 1150, 2065
TseI GCWGC 3 cut(s) 237, 1255, 2060
Tsp45I GTSAC 3 cut(s) 625, 1150, 2065
TspGWI ACGGA 3 cut(s) 1537, 1744, 2393
TspRI CASTG 5 cut(s) 1377, 1636, 1906, 2227, 2602
VpaK11BI GGWCC 2 cut(s) 315, 1071
VspI ATTAAT 3 cut(s) 1137, 1170, 2364
XagI CCTNNNNNAGG 1 cut(s) 2931
XapI RAATTY 7 cut(s) 277, 1043, 1184, 1717, 2591, 2654, 3013
XceI RCATGY 5 cut(s) 1200, 1557, 1821, 2492, 2920
XmiI GTMKAC 5 cut(s) 947, 1225, 1332, 2155, 2416
XmnI GAANNNNTTC 3 cut(s) 771, 2334, 2847
XspI CTAG 5 cut(s) 872, 1538, 1961, 1988, 3186
Zsp2I ATGCAT 3 cut(s) 2125, 2173, 2305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.