MD16G1250500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008661
Physical Location & Seq
Forward (+)
28046071 .. 28046782
712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1250500.v1.1.491

Sequence Viewer

Length: 501 bp
ATGAAGGACAAGCCTGCAACCGTCACTTTGCCTGTTACTCTTTTTAACACACTTATAAAGAATGGTAGAGTTTTGGATGCATATAAACTTGTCATGGTGGCTGGAGATGGTCTATCTATTCTGGATGCATTTGATTATTCACTTATAGTTGATAGTCTTTGTAAATTAGGATATATCAATGAGGCATTAGATCTGTGTAGTTTTGCTAAAAATAAATGGGTGACCCTTGATATCATCATTTATAATTCAGTTATAAATGGATTATGCCGCCAAGGCCACCTTGTTGAAGCATTTTGGCTATTTGATTCATTAGAGAGAATTAATTTGGTACCCACGGAAATCACTTATGCTACATTGATTGATGCCTTACGTAGAGAGGGGTTTCTTCTGGATGCCAAGGAGTTTTTTGAGAGGATGGTTCTCAAGGGCTTTAAACCAAATACACATGTATACAACTCGATTATTGATGGTTATTACAAAATTGGAGATATGATGCCTTGA

Protein Analysis

167

Amino Acids

18.82

Weight (kDa)

5.35

Isoelectric Point (pI)

21.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 76 - 103 3.1e-08 PPR repeat
PPR_2 PF13041 77 - 123 2.2e-12 PPR repeat family
PPR PF01535 80 - 107 9e-07 PPR repeat
PPR_3 PF13812 100 - 157 5.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 108 - 140 9.9e-07 PPR repeat
PPR_2 PF13041 111 - 160 1.2e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 56, 243, 254
Acc65I GGTACC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 450
AciI CCGC 1 cut(s) 268
AfaI GTAC 1 cut(s) 330
AflIII ACRYGT 1 cut(s) 445
AgsI TTSAA 1 cut(s) 287
AoxI GGCC 1 cut(s) 274
AseI ATTAAT 1 cut(s) 321
Asp718I GGTACC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 232
BanI GGYRCC 1 cut(s) 328
BccI CCATC 3 cut(s) 101, 409, 461
BglI GCCNNNNNGGC 1 cut(s) 273
BglII AGATCT 1 cut(s) 190
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 5 cut(s) 67, 115, 352, 382, 483
BpmI CTGGAG 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 407
BsaAI YACGTR 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 271, 333, 396
BseDI CCNNGG 3 cut(s) 271, 333, 396
BseGI GGATG 4 cut(s) 82, 130, 397, 420
BshFI GGCC 1 cut(s) 276
BshNI GGYRCC 1 cut(s) 328
BsnI GGCC 1 cut(s) 276
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 268
BspANI GGCC 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 271, 333, 396
BssMI GATC 1 cut(s) 190
BssNAI GTATAC 1 cut(s) 451
BssT1I CCWWGG 2 cut(s) 271, 396
Bst1107I GTATAC 1 cut(s) 451
Bst4CI ACNGT 1 cut(s) 22
BstBAI YACGTR 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 15
BstDSI CCRYGG 1 cut(s) 333
BstEII GGTNACC 1 cut(s) 220
BstF5I GGATG 4 cut(s) 82, 130, 397, 420
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNSI RCATGY 1 cut(s) 449
BstPI GGTNACC 1 cut(s) 220
BstSNI TACGTA 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BstZ17I GTATAC 1 cut(s) 451
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 82, 130, 397, 420
Cac8I GCNNGC 1 cut(s) 15
Csp6I GTAC 1 cut(s) 329
CviAII CATG 2 cut(s) 94, 446
CviJI RGCY 5 cut(s) 13, 101, 276, 298, 429
CviKI_1 RGCY 5 cut(s) 13, 101, 276, 298, 429
CviQI GTAC 1 cut(s) 329
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DraI TTTAAA 1 cut(s) 433
Eco105I TACGTA 1 cut(s) 371
Eco130I CCWWGG 2 cut(s) 271, 396
Eco32I GATATC 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 220
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 271, 396
EcoT22I ATGCAT 2 cut(s) 82, 130
ErhI CCWWGG 2 cut(s) 271, 396
FaeI CATG 2 cut(s) 97, 449
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 2 cut(s) 93, 445
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 4 cut(s) 89, 137, 404, 427
Fsp4HI GCNGC 1 cut(s) 268
GluI GCNGC 1 cut(s) 268
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 276
Hin1II CATG 2 cut(s) 97, 449
HinfI GANTC 1 cut(s) 305
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 451
Hpy188III TCNNGA 2 cut(s) 122, 389
Hpy8I GTNNAC 1 cut(s) 451
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 3 cut(s) 17, 80, 128
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 2 cut(s) 97, 449
KpnI GGTACC 1 cut(s) 332
Kzo9I GATC 1 cut(s) 190
LpnPI CCDG 5 cut(s) 27, 45, 87, 107, 374
LweI GCATC 5 cut(s) 67, 115, 352, 382, 483
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 3 cut(s) 22, 34, 220
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 1 cut(s) 377
MflI RGATCY 1 cut(s) 190
MluCI AATT 5 cut(s) 164, 244, 318, 322, 480
MnlI CCTC 3 cut(s) 175, 370, 405
Mph1103I ATGCAT 2 cut(s) 82, 130
MseI TTAA 3 cut(s) 45, 321, 432
MwoI GCNNNNNNNGC 1 cut(s) 273
NdeII GATC 1 cut(s) 190
NlaIII CATG 2 cut(s) 97, 449
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 2 cut(s) 22, 220
NsiI ATGCAT 2 cut(s) 82, 130
NspI RCATGY 1 cut(s) 449
PciI ACATGT 1 cut(s) 445
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 1 cut(s) 269
Ppu21I YACGTR 1 cut(s) 371
PscI ACATGT 1 cut(s) 445
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 3 cut(s) 56, 243, 254
PspEI GGTNACC 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 330
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 45, 321, 432
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 1 cut(s) 190
SetI ASST 2 cut(s) 282, 373
SfaNI GCATC 5 cut(s) 67, 115, 352, 382, 483
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SnaBI TACGTA 1 cut(s) 371
Sse9I AATT 5 cut(s) 164, 244, 318, 322, 480
SsiI CCGC 1 cut(s) 268
StyI CCWWGG 2 cut(s) 271, 396
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 458
TasI AATT 5 cut(s) 164, 244, 318, 322, 480
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 3 cut(s) 45, 321, 432
Tru9I TTAA 3 cut(s) 45, 321, 432
TseFI GTSAC 2 cut(s) 22, 220
Tsp45I GTSAC 2 cut(s) 22, 220
TspDTI ATGAA 2 cut(s) 17, 297
TspGWI ACGGA 1 cut(s) 350
VspI ATTAAT 1 cut(s) 321
XceI RCATGY 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 450
Zsp2I ATGCAT 2 cut(s) 82, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.