Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008661

Physical Location & Seq

Genomic Coordinates Forward (+)

14879299 .. 14888926

Gene Structure 9628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00344750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3186 bp

ATGCCTAAGCTTTCCTCTACACTCTTCTACACCAAACCCAGCCGATCCTTGTCTTCATCTTCTTCTTCTCCTCGTAAAAGTCTTCCAAAACTCCCACCTCTACAAACTTTACTCAAACGTGGCTTCACTCCCACTCTCAATTCCATCATCCAATTCCTTCTCTTTCTTTCACATACCCGCAGATTCAACACAGTCATCCACTTCCTTTCTCAAATGGAGTCGAACCAAATCCAAGGCAACTCCCAAACTCGCTCAATTGTCACATGGGCTCTCCTAAAACTGCACAAATATGAAGATGCAGAGCATTTCATGAGGACCCAGATGGCCAAAGCTTCAAATTTTCCGAGGAATCGCATGTGGGACACTCTCATTCAAGGTCTCTGTATCAACCAGAAAGACCCAGATAAGGCTTTGTTGGTATTACGGGATTGCTTGAGAAAATATGGTACCTTTCCTTCTTCTTTCACTTTTTGCTCGTTGATTTATAGATTTAGCTATAAGGGAGATATGAGTAAGGCAATTGAGGTTGTGGAGTTGATGACAGATGAGAAAATCAACTACCCATTTAACAATTTTGTTTGTAGTTCAGTGATTTCTGGGTTTTGTAAAATTGGAAAGCCAGAAATTGCTGTTGAGTTTTTTGAGAATGCCCTAAAGTCTGGAGCTTTGCAGCCTAATGTTGTGGTCTACACAGCACTTGTAGGTGCTCTTTGTAAATTAGGAAGAGTTAGTGAGGTTTGTGATTTAGTTTGTAAAATGGAGAAGGAAGGATTGGTATTCGATGTTGTTTTCTATAATAGTTGGATTTGTGGCTATATTTCGGAAGGGAGTCTAATGGAGGTGTTTCGGAAGAAGAGACAGATGGTAGATAAAGGCATAAGACCAGATACAGTTAGCTATACTATACTCGTAGATGGGTTTTCGAAGCTTGGAGATGTGGAAAAGACAATTGGTTTATTGAAAAAGATGAGGAATAGTGGGCTAGAACCCAATTTTATTACTTATACCGCCATCATGTTAGGATTCTGCAAGAAAGGAAAATTGGAGGAGGCATTTGCTATTTTCAAGATGGTTGAAGATTTGGGAATTGAAGTCGATGAATTTATGTATGCAACTTTAATTCACGGGTTTTGCATGAGAGGGGATCTTGATGGTGTTTTTCGTCTGCTAGATGAAATGGAAATGAAGGGGATTAATCCAAGCATTGTTACATATAATACGGTAATTAATGGATTATGCAAATTTGGGAGGACTGCCGAGGCTGAGAAGATCTCAAAGGGCATATCTGGAGATACCATTACATATAGTACATTGCTACATGGGTATATTGAGGAAGAAAATACCTCTGGGATTTTGGAAACCAAGAGGAGATTGGAGGAAGCTGGAGTCTTTATGGATGTTGTTATGTGTAATATACTTATTAAAGCACTATTCATGGTGGGGGCATTTGAGGATGCTTACATACTGTACAAGGGAATGCCAGAGAAGGGTTTGACTGCAAATTCCATTACATACTGTACAATGATTGATGGGTACTGTAAAGTTGGTCGAATTGATGAGGCACTTGAGATATTTGATGAGTTCAGAAGGACACCACTTTCCTCAGTTGCATGTTATAATTGTATAATAAATTGGCTCTGTAAGCAGGGCATGGTAGATATGGCCACGGAGGTGTTTATTGAACTTAATCAGAAAAGCTTAACTTTGGATGATGGTATCTGCAAAATGCTTTTGAAGGCAACTTTTAAACAAAAAAGTGCAACTGGAGTTTTAAATTTGGTTCACAGAGTGGAAAATTTGAGGCCAGGTATGTATGATGTTATGAGCAATGTTGCGATCAGTTTCTTGTGCAAGAGAGGGTTTCATGAGTCTGCATTTGAAGTGTATGCAGTGATGAGGAGGGAAGGGTCGGTTGCAACGAGAAAAACTTATTATTCAATTTTAGAAGGTCTAATTAATGATGGAAAAGAGTGGCTAACTCTGCCTCTTTTAAACATCTTTCTAAAAGAATATGGACTAGTTGAACCTAAAGTAAGCCAGATTCTTGCTTACCACATGTGCCTGAAGGATGTCAATGATGCTTTCTGCTTTTTAGAAAAGATCAAGGACAGACCTACAGCCATTACCTTGCCTATTTCTCTTTTCAAGACACTTATGAAGAAGGGTAAAGTTTTGGTTGCATATCAGCTTTTCATGGAGGCTGAAGATAGTGTACCCGTTCTGGATGCATTTGATTATTCGCACATGGTTGATGGCCTTTGCAAGGGAGGATATATCAGTGAGGCACTAGATCTCTGTGGTTTTGCTAAATCTAAGGGAATCACCCTGAATATTATTGCTTATAATTCTGTTATAAATGGATTGTGCCGCCAAGGTCATCTTGTTGATGCATTTCGGCTATTTGATTCATTAGAGAGAATTAATTTGGTACCCTCAGAAATCACTTATGCTACATTGATTGATTCCCTACGTAGAGAGGGGTTTCTGCTTGATGCAAAGCAATTATTTGAAAGCATGGTCCTCAAGGGGTTTAAACTGAATACACATGTATATAACTCTATTATTGATGGTTTTTGCAAAATTGGACATATGGAGGATGCCTTGAAGCTTCTTTGTGAATTGGACATGAAATCTCTTAGGCCTGATGAATTCACTGTTACTATTGTCATCAATGGCTTTTGCCAAAAGGGAAGTATCCAAGAAGCAGTGACTTGCCTTTCTCCATACTCAATTGAAAACATGTTAAAGGATTTCACTTGCTATTTAAATTCTGGAAAATGCTTGTATGGAAAATGCATGTCCGTCAGGTCCAGAAGTATAATCTTCCAATTTGTACAATATCTCTCAGTGGTGGCGAGCCATGAAGAGAAAAAGTTTAGAAACTTAATGATGGATCGTCGCCACCGATTTCTGGTATTCTTGAAACAAAGAGGAAAATTAGATGAGTTTAATAAGTTGACTGAAACTTGTGCATCCTTTTTATTTCCATCATCTTCTTCGACCAAATCTCCCAAACGAAGGTCTTGGAGGGACAGAAGGCAACCTATCAGCTATATTTTAAAGAGACAAGCCCGTTCTAAAGCGGACTTGCGGACTTTGCATTGTTCCAACGGCTCCTGCATTTCAGCAAATCTTCTATGTAACCTTATCATCATGAATGTGCTGGCAGTTGGCAGTAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1061

Amino Acids

120.19

Weight (kDa)

8.77

Isoelectric Point (pI)

38.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRP-S27	PF10037	127 - 189	1e-06	Mitochondrial 28S ribosomal protein S27
PPR_2	PF13041	225 - 261	6.7e-06	PPR repeat family
PPR_2	PF13041	261 - 309	2.7e-10	PPR repeat family
PPR_long	PF17177	277 - 374	1.7e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	285 - 339	4.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	291 - 324	3.9e-07	PPR repeat
PPR_2	PF13041	297 - 344	3.1e-11	PPR repeat family
PPR_1	PF12854	327 - 356	1.4e-08	PPR repeat
PPR_2	PF13041	330 - 378	2.3e-10	PPR repeat family
TPR_24	PF23276	346 - 491	1.8e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	354 - 410	1.4e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	362 - 394	5.4e-08	PPR repeat
PPR_2	PF13041	366 - 414	6.5e-15	PPR repeat family
PPR_1	PF12854	396 - 423	1.1e-09	PPR repeat
PPR_2	PF13041	466 - 514	8.9e-13	PPR repeat family
PPR_1	PF12854	496 - 527	1.8e-11	PPR repeat
PPR_2	PF13041	501 - 529	6.8e-06	PPR repeat family
PPR	PF01535	503 - 530	3.6e-08	PPR repeat
PPR_2	PF13041	535 - 570	1.6e-06	PPR repeat family
PPR_1	PF12854	772 - 803	1.9e-09	PPR repeat
PPR_2	PF13041	777 - 823	5.2e-12	PPR repeat family
PPR_2	PF13041	818 - 860	2e-06	PPR repeat family
PPR_1	PF12854	842 - 873	1.2e-08	PPR repeat
PPR_2	PF13041	847 - 895	7.7e-15	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57250
fragaria_vesca	FvH4_4g07420
malus_domestica	MD13G1243700.v1.1 MD16G1250300.v1.1 MD16G1250400.v1.1 MD16G1250500.v1.1 MD16G1250600.v1.1
prunus_persica	Prupe.1G077100_v2.0.a1
pyrus_communis	pycom13g21510 pycom16g21000
rosa_chinensis	RchiOBHm_Chr2g0131261 RchiOBHm_Chr4g0399881
rosa_laevigata	RLG00000009236
rosa_multiflora	Rmu_sc0014676.1_g000003
rosa_roxburghii	Rroxscaffold_5G00344750
rosa_rugosa	Rorug04G0019700
rosa_samantha	Rh2BG356200 Rh4AG096800 Rh4BG094300 Rh4CG105900 Rh4DG089200 Rh4DG091000
rosa_wichuraiana	Rw4G008000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1617, 2343, 2354
Acc65I	GGTACC	2 cut(s)	446, 2428
AccB1I	GGYRCC	2 cut(s)	446, 2428
AccI	GTMKAC	1 cut(s)	687
AciI	CCGC	5 cut(s)	178, 1008, 2368, 3083, 3091
AclWI	GGATC	3 cut(s)	39, 1152, 2901
AcoI	YGGCCR	2 cut(s)	324, 1662
AcsI	RAATTY	8 cut(s)	337, 1100, 1241, 1501, 1774, 1795, 2648, 2767
AcuI	CTGAAG	2 cut(s)	2084, 2223
AdeI	CACNNNGTG	1 cut(s)	1789
AfaI	GTAC	8 cut(s)	448, 1309, 1469, 1519, 1535, 2214, 2430, 2835
AfiI	CCNNNNNNNGG	5 cut(s)	406, 1487, 2263, 2911, 3018
AflIII	ACRYGT	3 cut(s)	2055, 2545, 2739
AhlI	ACTAGT	1 cut(s)	2017
AjnI	CCWGG	1 cut(s)	1804
AjuI	GAANNNNNNNTTGG	6 cut(s)	755, 787, 933, 965, 1025, 1057
AleI	CACNNNNGTG	1 cut(s)	1670
Alw21I	GWGCWC	1 cut(s)	709
Alw26I	GTCTC	3 cut(s)	383, 850, 3058
AlwI	GGATC	3 cut(s)	39, 1152, 2901
AoxI	GGCC	5 cut(s)	324, 1662, 1802, 2254, 2639
ApeKI	GCWGC	1 cut(s)	670
ApoI	RAATTY	8 cut(s)	337, 1100, 1241, 1501, 1774, 1795, 2648, 2767
AseI	ATTAAT	4 cut(s)	1194, 1227, 1956, 2421
Asp718I	GGTACC	2 cut(s)	446, 2428
AspS9I	GGNCC	3 cut(s)	315, 2518, 2808
AsuHPI	GGTGA	1 cut(s)	2314
AsuII	TTCGAA	1 cut(s)	923
AvaII	GGWCC	3 cut(s)	315, 2518, 2808
BalI	TGGCCA	2 cut(s)	326, 1664
BanI	GGYRCC	2 cut(s)	446, 2428
BanII	GRGCYC	1 cut(s)	271
BarI	GAAGNNNNNNTAC	4 cut(s)	439, 471, 2196, 2228
BbsI	GAAGAC	2 cut(s)	45, 74
Bbv12I	GWGCWC	1 cut(s)	709
BbvI	GCAGC	1 cut(s)	682
BceAI	ACGGC	1 cut(s)	3127
BciT130I	CCWGG	1 cut(s)	1806
BciVI	GTATCC	1 cut(s)	2705
BcoDI	GTCTC	3 cut(s)	383, 850, 3058
BcuI	ACTAGT	1 cut(s)	2017
BfaI	CTAG	4 cut(s)	983, 1169, 2018, 2288
BfmI	CTRYAG	1 cut(s)	2115
BfuI	GTATCC	1 cut(s)	2705
BglII	AGATCT	2 cut(s)	1269, 2290
BisI	GCNGC	2 cut(s)	671, 2368
BlsI	GCNGC	2 cut(s)	672, 2369
Bme1390I	CCNGG	1 cut(s)	1806
Bme18I	GGWCC	3 cut(s)	315, 2518, 2808
BmgT120I	GGNCC	3 cut(s)	315, 2518, 2808
BmiI	GGNNCC	4 cut(s)	317, 448, 2430, 3115
BmrFI	CCNGG	1 cut(s)	1806
BmsI	GCATC	8 cut(s)	286, 1444, 2068, 2215, 2377, 2482, 2587, 2981
BpiI	GAAGAC	2 cut(s)	45, 74
BplI	GAGNNNNNCTC	2 cut(s)	1256, 1288
BpmI	CTGGAG	4 cut(s)	681, 1308, 1404, 1785
Bpu10I	CCTNAGC	1 cut(s)	6
Bpu14I	TTCGAA	1 cut(s)	923
BpuEI	CTTGAG	3 cut(s)	454, 1586, 2507
BsaAI	YACGTR	1 cut(s)	2471
BsaI	GGTCTC	1 cut(s)	383
BsaJI	CCNNGG	5 cut(s)	232, 344, 1257, 1665, 2371
BsaXI	ACNNNNNCTCC	2 cut(s)	2992, 3022
Bsc4I	CCNNNNNNNGG	5 cut(s)	406, 1487, 2263, 2911, 3018
Bse1I	ACTGG	1 cut(s)	1768
Bse3DI	GCAATG	2 cut(s)	1310, 1834
BseBI	CCWGG	1 cut(s)	1806
BseDI	CCNNGG	5 cut(s)	232, 344, 1257, 1665, 2371
BseGI	GGATG	9 cut(s)	147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2972
BseLI	CCNNNNNNNGG	5 cut(s)	406, 1487, 2263, 2911, 3018
BseMI	GCAATG	2 cut(s)	1310, 1834
BseMII	CTCAG	4 cut(s)	1254, 1617, 2448, 2859
BseNI	ACTGG	1 cut(s)	1768
BseRI	GAGGAG	4 cut(s)	60, 1061, 1381, 1912
BseXI	GCAGC	1 cut(s)	682
BseYI	CCCAGC	1 cut(s)	38
BsgI	GTGCAG	1 cut(s)	266
BshFI	GGCC	5 cut(s)	326, 1664, 1804, 2256, 2641
BshNI	GGYRCC	2 cut(s)	446, 2428
BsiHKAI	GWGCWC	1 cut(s)	709
BslFI	GGGAC	2 cut(s)	374, 3044
BslI	CCNNNNNNNGG	5 cut(s)	406, 1487, 2263, 2911, 3018
BsmAI	GTCTC	3 cut(s)	383, 850, 3058
BsmFI	GGGAC	2 cut(s)	374, 3044
BsmI	GAATGC	2 cut(s)	652, 1482
BsnI	GGCC	5 cut(s)	326, 1664, 1804, 2256, 2641
Bso31I	GGTCTC	1 cut(s)	383
Bsp119I	TTCGAA	1 cut(s)	923
Bsp1286I	GDGCHC	2 cut(s)	271, 709
Bsp1407I	TGTACA	3 cut(s)	1467, 1517, 2833
Bsp143I	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
BspACI	CCGC	5 cut(s)	178, 1008, 2368, 3083, 3091
BspANI	GGCC	5 cut(s)	326, 1664, 1804, 2256, 2641
BspCNI	CTCAG	4 cut(s)	1255, 1616, 2447, 2858
BspHI	TCATGA	3 cut(s)	309, 1864, 3153
BspLI	GGNNCC	4 cut(s)	317, 448, 2430, 3115
BspPI	GGATC	3 cut(s)	39, 1152, 2901
BspT104I	TTCGAA	1 cut(s)	923
BspT107I	GGYRCC	2 cut(s)	446, 2428
BspTNI	GGTCTC	1 cut(s)	383
BsrDI	GCAATG	2 cut(s)	1310, 1834
BsrGI	TGTACA	3 cut(s)	1467, 1517, 2833
BsrI	ACTGG	1 cut(s)	1768
BssECI	CCNNGG	5 cut(s)	232, 344, 1257, 1665, 2371
BssMI	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
BssT1I	CCWWGG	2 cut(s)	232, 2371
Bst2UI	CCWGG	1 cut(s)	1806
Bst4CI	ACNGT	7 cut(s)	193, 892, 1222, 1467, 1517, 1538, 2656
Bst6I	CTCTTC	4 cut(s)	29, 718, 848, 2859
BstAUI	TGTACA	3 cut(s)	1467, 1517, 2833
BstBAI	YACGTR	1 cut(s)	2471
BstBI	TTCGAA	1 cut(s)	923
BstC8I	GCNNGC	2 cut(s)	2857, 3165
BstDEI	CTNAG	7 cut(s)	6, 1263, 1603, 2313, 2434, 2636, 2845
BstDSI	CCRYGG	1 cut(s)	1665
BstENI	CCTNNNNNAGG	1 cut(s)	2261
BstF5I	GGATG	9 cut(s)	147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2972
BstKTI	GATC	7 cut(s)	47, 1147, 1272, 1839, 2103, 2293, 2896
BstMAI	GTCTC	3 cut(s)	383, 850, 3058
BstMBI	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
BstMWI	GCNNNNNNNGC	3 cut(s)	1642, 1981, 3097
BstNI	CCWGG	1 cut(s)	1806
BstNSI	RCATGY	6 cut(s)	358, 1614, 2059, 2549, 2743, 2800
BstSCI	CCNGG	1 cut(s)	1804
BstSFI	CTRYAG	1 cut(s)	2115
BstSNI	TACGTA	1 cut(s)	2471
BstV1I	GCAGC	1 cut(s)	682
BstV2I	GAAGAC	2 cut(s)	45, 74
BstX2I	RGATCY	3 cut(s)	1144, 1269, 2290
BstYI	RGATCY	3 cut(s)	1144, 1269, 2290
BsuI	GTATCC	1 cut(s)	2705
BsuRI	GGCC	5 cut(s)	326, 1664, 1804, 2256, 2641
BtgI	CCRYGG	1 cut(s)	1665
BtsCI	GGATG	9 cut(s)	147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2972
BtsI	GCAGTG	2 cut(s)	1896, 2712
BtsIMutI	CAGTG	6 cut(s)	594, 1896, 2284, 2652, 2712, 2853
Cac8I	GCNNGC	2 cut(s)	2857, 3165
CciI	TCATGA	3 cut(s)	309, 1864, 3153
Cfr13I	GGNCC	3 cut(s)	315, 2518, 2808
Csp6I	GTAC	8 cut(s)	447, 1308, 1468, 1518, 1534, 2213, 2429, 2834
CviQI	GTAC	8 cut(s)	447, 1308, 1468, 1518, 1534, 2213, 2429, 2834
DdeI	CTNAG	7 cut(s)	6, 1263, 1603, 2313, 2434, 2636, 2845
DpnI	GATC	7 cut(s)	46, 1146, 1271, 1838, 2102, 2292, 2895
DpnII	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
DraI	TTTAAA	6 cut(s)	1747, 1773, 1992, 2533, 2766, 3060
DraIII	CACNNNGTG	1 cut(s)	1789
EaeI	YGGCCR	2 cut(s)	324, 1662
Eam1104I	CTCTTC	4 cut(s)	29, 718, 848, 2859
EarI	CTCTTC	4 cut(s)	29, 718, 848, 2859
Eco105I	TACGTA	1 cut(s)	2471
Eco130I	CCWWGG	2 cut(s)	232, 2371
Eco147I	AGGCCT	1 cut(s)	2641
Eco24I	GRGCYC	1 cut(s)	271
Eco31I	GGTCTC	1 cut(s)	383
Eco47I	GGWCC	3 cut(s)	315, 2518, 2808
Eco57I	CTGAAG	2 cut(s)	2084, 2223
EcoNI	CCTNNNNNAGG	1 cut(s)	2261
EcoO109I	RGGNCCY	1 cut(s)	315
EcoRI	GAATTC	1 cut(s)	2648
EcoRII	CCWGG	1 cut(s)	1804
EcoT14I	CCWWGG	2 cut(s)	232, 2371
EcoT22I	ATGCAT	3 cut(s)	2230, 2392, 2798
EcoT38I	GRGCYC	1 cut(s)	271
ErhI	CCWWGG	2 cut(s)	232, 2371
FalI	AAGNNNNNCTT	4 cut(s)	1687, 1719, 2364, 2396
FaqI	GGGAC	2 cut(s)	374, 3044
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	1 cut(s)	2589
FblI	GTMKAC	1 cut(s)	687
Fnu4HI	GCNGC	2 cut(s)	671, 2368
FokI	GGATG	9 cut(s)	134, 182, 1409, 1466, 1721, 2081, 2237, 2609, 2959
FriOI	GRGCYC	1 cut(s)	271
Fsp4HI	GCNGC	2 cut(s)	671, 2368
FspBI	CTAG	4 cut(s)	983, 1169, 2018, 2288
GluI	GCNGC	2 cut(s)	671, 2368
GsaI	CCCAGC	1 cut(s)	42
GsuI	CTGGAG	4 cut(s)	681, 1308, 1404, 1785
HaeIII	GGCC	5 cut(s)	326, 1664, 1804, 2256, 2641
HincII	GTYRAC	1 cut(s)	2958
HindII	GTYRAC	1 cut(s)	2958
HindIII	AAGCTT	5 cut(s)	8, 330, 926, 1696, 2606
HphI	GGTGA	1 cut(s)	2314
Hpy166II	GTNNAC	4 cut(s)	688, 1783, 2213, 2958
Hpy188I	TCNGA	6 cut(s)	345, 823, 849, 1586, 1692, 2437
Hpy8I	GTNNAC	4 cut(s)	688, 1783, 2213, 2958
Hpy99I	CGWCG	1 cut(s)	2901
HpyCH4III	ACNGT	7 cut(s)	193, 892, 1222, 1467, 1517, 1538, 2656
HpyCH4IV	ACGT	2 cut(s)	118, 2470
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1642, 1981, 3097
HpyF3I	CTNAG	7 cut(s)	6, 1263, 1603, 2313, 2434, 2636, 2845
HpySE526I	ACGT	2 cut(s)	118, 2470
KpnI	GGTACC	2 cut(s)	450, 2432
Kzo9I	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
LmnI	GCTCC	2 cut(s)	662, 3119
Lsp1109I	GCAGC	1 cut(s)	682
LweI	GCATC	8 cut(s)	286, 1444, 2068, 2215, 2377, 2482, 2587, 2981
MaeI	CTAG	4 cut(s)	983, 1169, 2018, 2288
MaeII	ACGT	2 cut(s)	118, 2470
MaeIII	GTNAC	5 cut(s)	259, 1207, 2656, 2707, 3140
MalI	GATC	7 cut(s)	46, 1146, 1271, 1838, 2102, 2292, 2895
MboI	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
MfeI	CAATTG	4 cut(s)	255, 519, 948, 2730
MflI	RGATCY	3 cut(s)	1144, 1269, 2290
MhlI	GDGCHC	2 cut(s)	271, 709
MlsI	TGGCCA	2 cut(s)	326, 1664
MluNI	TGGCCA	2 cut(s)	326, 1664
MlyI	GAGTC	4 cut(s)	227, 838, 1395, 1877
MmeI	TCCRAC	2 cut(s)	782, 3132
Mox20I	TGGCCA	2 cut(s)	326, 1664
Mph1103I	ATGCAT	3 cut(s)	2230, 2392, 2798
MscI	TGGCCA	2 cut(s)	326, 1664
MslI	CAYNNNNRTG	3 cut(s)	288, 1670, 3158
Msp20I	TGGCCA	2 cut(s)	326, 1664
MspR9I	CCNGG	1 cut(s)	1806
MssI	GTTTAAAC	1 cut(s)	2533
MunI	CAATTG	4 cut(s)	255, 519, 948, 2730
Mva1269I	GAATGC	2 cut(s)	652, 1482
MvaI	CCWGG	1 cut(s)	1806
MwoI	GCNNNNNNNGC	3 cut(s)	1642, 1981, 3097
NdeI	CATATG	1 cut(s)	2589
NdeII	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
NlaIV	GGNNCC	4 cut(s)	317, 448, 2430, 3115
NmeAIII	GCCGAG	1 cut(s)	1282
NmuCI	GTSAC	2 cut(s)	259, 2707
NsiI	ATGCAT	3 cut(s)	2230, 2392, 2798
NspI	RCATGY	6 cut(s)	358, 1614, 2059, 2549, 2743, 2800
NspV	TTCGAA	1 cut(s)	923
OliI	CACNNNNGTG	1 cut(s)	1670
PagI	TCATGA	3 cut(s)	309, 1864, 3153
PceI	AGGCCT	1 cut(s)	2641
PciI	ACATGT	3 cut(s)	2055, 2545, 2739
PcsI	WCGNNNNNNNCGW	2 cut(s)	1916, 2902
PctI	GAATGC	2 cut(s)	652, 1482
PfeI	GAWTC	7 cut(s)	183, 349, 1023, 2041, 2319, 2405, 2462
PkrI	GCNGC	2 cut(s)	672, 2369
PleI	GAGTC	4 cut(s)	226, 837, 1394, 1876
PmeI	GTTTAAAC	1 cut(s)	2533
PpsI	GAGTC	4 cut(s)	226, 837, 1394, 1876
Ppu21I	YACGTR	1 cut(s)	2471
PpuMI	RGGWCCY	1 cut(s)	315
PscI	ACATGT	3 cut(s)	2055, 2545, 2739
PshBI	ATTAAT	4 cut(s)	1194, 1227, 1956, 2421
PsiI	TTATAA	3 cut(s)	1617, 2343, 2354
Psp5II	RGGWCCY	1 cut(s)	315
Psp6I	CCWGG	1 cut(s)	1804
PspFI	CCCAGC	1 cut(s)	38
PspGI	CCWGG	1 cut(s)	1804
PspN4I	GGNNCC	4 cut(s)	317, 448, 2430, 3115
PspPI	GGNCC	3 cut(s)	315, 2518, 2808
PspPPI	RGGWCCY	1 cut(s)	315
PsuI	RGATCY	3 cut(s)	1144, 1269, 2290
RsaI	GTAC	8 cut(s)	448, 1309, 1469, 1519, 1535, 2214, 2430, 2835
RsaNI	GTAC	8 cut(s)	447, 1308, 1468, 1518, 1534, 2213, 2429, 2834
RseI	CAYNNNNRTG	3 cut(s)	288, 1670, 3158
SatI	GCNGC	2 cut(s)	671, 2368
Sau3AI	GATC	7 cut(s)	44, 1144, 1269, 1836, 2100, 2290, 2893
Sau96I	GGNCC	3 cut(s)	315, 2518, 2808
SchI	GAGTC	4 cut(s)	227, 838, 1395, 1877
ScrFI	CCNGG	1 cut(s)	1806
SduI	GDGCHC	2 cut(s)	271, 709
SfaNI	GCATC	8 cut(s)	286, 1444, 2068, 2215, 2377, 2482, 2587, 2981
SfcI	CTRYAG	1 cut(s)	2115
SfuI	TTCGAA	1 cut(s)	923
SinI	GGWCC	3 cut(s)	315, 2518, 2808
SmiI	ATTTAAAT	1 cut(s)	2766
SmiMI	CAYNNNNRTG	3 cut(s)	288, 1670, 3158
SmlI	CTYRAG	3 cut(s)	433, 1565, 2522
SmoI	CTYRAG	3 cut(s)	433, 1565, 2522
SnaBI	TACGTA	1 cut(s)	2471
SpeI	ACTAGT	1 cut(s)	2017
SseBI	AGGCCT	1 cut(s)	2641
SsiI	CCGC	5 cut(s)	178, 1008, 2368, 3083, 3091
SspI	AATATT	1 cut(s)	2332
SspMI	CTAG	4 cut(s)	983, 1169, 2018, 2288
StuI	AGGCCT	1 cut(s)	2641
StyD4I	CCNGG	1 cut(s)	1804
StyI	CCWWGG	2 cut(s)	232, 2371
SwaI	ATTTAAAT	1 cut(s)	2766
TaaI	ACNGT	7 cut(s)	193, 892, 1222, 1467, 1517, 1538, 2656
TaiI	ACGT	2 cut(s)	121, 2473
TaqI	TCGA	6 cut(s)	221, 780, 923, 1095, 1549, 2999
TatI	WGTACW	4 cut(s)	1307, 1467, 1517, 2833
TauI	GCSGC	1 cut(s)	2370
TfiI	GAWTC	7 cut(s)	183, 349, 1023, 2041, 2319, 2405, 2462
TscAI	CASTG	6 cut(s)	594, 1896, 2284, 2659, 2712, 2853
TseFI	GTSAC	2 cut(s)	259, 2707
TseI	GCWGC	1 cut(s)	670
Tsp45I	GTSAC	2 cut(s)	259, 2707
TspGWI	ACGGA	2 cut(s)	1682, 2791
TspRI	CASTG	6 cut(s)	594, 1896, 2284, 2659, 2712, 2853
VpaK11BI	GGWCC	3 cut(s)	315, 2518, 2808
VspI	ATTAAT	4 cut(s)	1194, 1227, 1956, 2421
XagI	CCTNNNNNAGG	1 cut(s)	2261
XapI	RAATTY	8 cut(s)	337, 1100, 1241, 1501, 1774, 1795, 2648, 2767
XceI	RCATGY	6 cut(s)	358, 1614, 2059, 2549, 2743, 2800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1369
XmiI	GTMKAC	1 cut(s)	687
XspI	CTAG	4 cut(s)	983, 1169, 2018, 2288
Zsp2I	ATGCAT	3 cut(s)	2230, 2392, 2798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.