Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0008661

Physical Location & Seq

Genomic Coordinates Forward (+)

16091636 .. 16098376

Gene Structure 6741 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG094300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3291 bp

ATGCCTAAGCTTTCCTCTACACTCTTCTACACCAAACCCAGCCGATCCTTGTCTTCATCTTCTTCTTCTTCTTCTCGTAACAGTCTTCCAAAACTCCCACCTCTTCAAACTTTACTCAAACGTGGCTTCACTCCCACTCTCAATTCCATCATCCAATTCCTTCTCTTTCTTTCACATACCCGCAGATTCAACACAGTCATCCACTTCTTCTCTCAAATGGAGTCGAACCAAATCAAAGGCAACTCCCAAACTCACTCAATTCTCACATGGGCTCTCCTTAAACTGCACAAATATGAAGATGCAGCGCATTTCATGAGGACCCAGATGGCCAAAGCTTCAAATTTTCCGAGGAATCGCATGTGGGACACTCTCATTCAAGGTCTCTGTATCAACCAGAAAGACCCAGATAAGGCTTTGTTGGTATTACGGGATTGCTTGAGAATATATGGTACCTTTCCTTCTTCTTTCACTTTTTGCTCGTTAATTTATAGATTTAGCTCTATGGGGGATATGAGTAAGGCAATTGAGGTTGTGGAGTTGATGACAGATGAGAAAATCAACTACCCATTTAACAATTTTGTTTGTAGTTCAGTGATTTCTGGGTTTTGTAAAATTGGAAAGCCAGAAATAGCTGTTGAGTTTTTTGAGAATGCCCTAAAGTCTGGATCTTTGCAGCCTAATGTTGTGGTATACACAGCACTTGTAGGTGCTCTTTGTAAATTAGGAAGAGTTAGTGAGGATTGTGATTTAGTTTGTAAAATGGAGAAGGAAGGATTGGCATTCGATGTTGTTTTCTATAGTAGTTGGATTTGTGGTTATATTTCGGAAGGGAGTCTAATGGAGGTGTTTCGGAAGAAGAGACAGATGGTAGATAAAGGCATAAGACCAGATACAGTTAGCTATACTATACTCGTAGATGGGTTTTCGAAGCTTGGAGATGTGGAAAAGGCAATTGGCTTTTTGAAAAAGATGAGGAAAGGTGGGCTAGAACCCAGTTTGATTACTTATACCACCATCATGTTGGGATTCTGCGAGAAAGGAAAACTGGAGGAGGCATTTGCTATTTACAAGATGGTCGAAGATTTGGAAATTGAAGTTGATGAATTTATGTATGCTACTTTAATTCACGGGTTTTGTATGAGAGGGGATCTTGATGGTGTTTTTCGTCTGCTAGATGAAATGGAAAAGAAGGGGATTAATCCAAGCATTGTTACATATAATACGGTAATTAATGGATTATGCAAATTTGGGAGGACTGCGGAGGCTGAGAAGATCTCAAAGGGCATATCTGGAGATACCATTACATATAGTACATTGCTACATGCGTATATTGAGGAAGAAAATACCTCTGGGATTTTGGAAACCAAGAGGAGATTGGAGGAAGCTGGAGTCTTTATGGATGTTGTTATGTGTAATATACTTATTAAAGCACTATTCATGGTGGGGGCATTTGAGGATGCTTACATACTGTACAAGGGAATGCCAGAGAAGGGTTTGACTGCAAATTCCATTACATACTGTACAATGATTGATGGGTACTGTAAAGTTGGTAGAATTGATGAGGCACTTGAGATATTTGATGAGTTCAGAAGGACACCACTTTCCTCAGTTGCATGTTATAATTGTATAATAAATTGGCTCTGTAAGCAGGGCATGGTAGATATGGCCACGGAGGTGTTTATTGAACTTAATCAGAAAAGCTTAACTTTGGATGATGGTATCTGCAAAATGCTTTTGAAGGCAACTTTTAAACAAAAAAGTGCAACTGGAGTTTTAAATTTGGTTCACAGAGTGGAAAATTTGAGGCCAGGTATGTATGATGTTATGAGCAATGTTGCAATCAGTTTCTTGTGCAAGAGAGGGTTTCATGAGTCTGCATTTGAAGTGTATGCAGTGATGAGGAGGGAAGGGTCGGTTGCAACGAGAAAAACTTATTATTCAATTTTAGAAGGTCTGATTAATGATGGAAAAGAGTGGCTAACTCTGCCTCTTTTAAACATCTTTCTAAAAGAATATGGACTAGTTGAACCTAAAGTAAGCCAGATTCTTGCTTACCACATGTGCCTGAACGATGTCAATGATGCTTTCTGCTTTTTAGAAAAGATCAAGGACAGACCTACCACCATTACCTTGCCTATTTCTCTTTTCAAGACACTTATGAAGAAGGGTAAAATTTTGGTTGCATATCAGCTTTTCATGGAGGCTGAAGATAGTGTACCCGTTCTGGATGCATTTGATTATTCGCACATGGTTGATGGCCTTTGCAAGGGAGGATATATCAGTGAGGCACTAGATCTCTGTGGTTTTGCTAAATCTAAGGGAATCACCCTGAATATTGTTGCTTATAATTCAGTTATAAATGGATTGTGCCGCCAAGGTCATCTTGTTGATGCATTTCGGCTATTTGATTCATTAGAGAGAATTAATTTGGTACCCTCAGAAATCACTTATGCTACATTGATTGATTCCCTACGTAGAGAGGGGTTTCTGCTTGATGCAAAGCAATTATTTGAAAGCATGGTCCTCAAGGGGTTTAAACCGAATACACATGTATATAACTCTATTATTGATGGTTTTTGCAAAATTGGACATATGGAGGATGCCTTGAAGCTTCTTTGTGAATTGGACATGAAATCTCTTAGGCCTGATGAATTCACTGTTACTATTGTCATCAATGGCTTTTGCCAAAAGGGTGATATGGAAGGGGCTCTAAATTTCTTCACTGAGTTCAAAAGAAATGGTACATCACCTGATTTTTTGGGTTTCTTAAATTTGATAAGAGGTTTATGTGCCAAGGGAAGGATGGAAGAAGCTAGAAGTATCTTGAGAGAAATGCTTCAGTCTCAGTCTGTCCTGGAGCTAATAAATAGAGTTGATGTTGAGCTGGAAACGGAGTCATTGGAAAGCTTGCTTGTTTCACTGTGCGAGCAAGGAAGTATCCAAGAAGCAGTGACTGTCCTTAATGAAATCGCGTCCATGTTTTTCCCTGTTCAGAGTTCATCTATTGATTGCCATCAATCTCATAAACTACAGAAGCCTTGTGACAGCGAGGATCCTAAAGCAGTTACTTCAAAATCTGTAACTTCCATCAATGCAGATTCGGGTATTCAGTTTTCTGGTCTGAAGAAAGTAGAGAAGGTGGCAAAAACTTGTGATGTTATAGGGAGTAGGTCTCAGTTTCATGACTTTGATTATTACTATAAGCAAATTGCTTCACTGTGTTCGATAGGGGAGCTACAAAAGGCTAGTGAATTAGTAAAGGAAATAGTTTCTAATTTGGGCAAGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1096

Amino Acids

123.17

Weight (kDa)

6.19

Isoelectric Point (pI)

38.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRP-S27	PF10037	128 - 190	6.7e-06	Mitochondrial 28S ribosomal protein S27
PPR_2	PF13041	262 - 310	9.5e-09	PPR repeat family
PPR_long	PF17177	278 - 394	5e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	286 - 340	1.3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	292 - 325	1.6e-08	PPR repeat
PPR_2	PF13041	297 - 344	1.3e-12	PPR repeat family
PPR	PF01535	299 - 328	1.7e-06	PPR repeat
PPR_1	PF12854	328 - 355	8.4e-07	PPR repeat
TPR_24	PF23276	352 - 492	5.3e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	358 - 411	1.4e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	364 - 395	2.4e-07	PPR repeat
PPR_2	PF13041	367 - 415	5e-16	PPR repeat family
PPR	PF01535	370 - 399	7.7e-06	PPR repeat
PPR_1	PF12854	397 - 424	1.1e-09	PPR repeat
PPR_2	PF13041	467 - 515	9.3e-13	PPR repeat family
PPR_1	PF12854	497 - 528	1.9e-11	PPR repeat
PPR_2	PF13041	502 - 530	7e-06	PPR repeat family
PPR	PF01535	504 - 531	3.7e-08	PPR repeat
PPR_2	PF13041	536 - 571	1.7e-06	PPR repeat family
PPR_1	PF12854	773 - 804	8.8e-10	PPR repeat
PPR_2	PF13041	778 - 824	1.4e-12	PPR repeat family
PPR_2	PF13041	812 - 861	2.1e-10	PPR repeat family
PPR_1	PF12854	843 - 874	5.5e-10	PPR repeat
PPR_2	PF13041	847 - 896	1.5e-15	PPR repeat family
PPR_1	PF12854	879 - 909	6.4e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57250
fragaria_vesca	FvH4_4g07420
malus_domestica	MD13G1243700.v1.1 MD16G1250300.v1.1 MD16G1250400.v1.1 MD16G1250500.v1.1 MD16G1250600.v1.1
prunus_persica	Prupe.1G077100_v2.0.a1
pyrus_communis	pycom13g21510 pycom16g21000
rosa_chinensis	RchiOBHm_Chr2g0131261 RchiOBHm_Chr4g0399881
rosa_laevigata	RLG00000009236
rosa_multiflora	Rmu_sc0014676.1_g000003
rosa_roxburghii	Rroxscaffold_5G00344750
rosa_rugosa	Rorug04G0019700
rosa_samantha	Rh2BG356200 Rh4AG096800 Rh4BG094300 Rh4CG105900 Rh4DG089200 Rh4DG091000
rosa_wichuraiana	Rw4G008000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1620, 2346, 2357
Acc65I	GGTACC	2 cut(s)	449, 2431
AccB1I	GGYRCC	2 cut(s)	449, 2431
AccI	GTMKAC	1 cut(s)	690
AccII	CGCG	1 cut(s)	2972
AciI	CCGC	3 cut(s)	181, 1259, 2371
AclWI	GGATC	5 cut(s)	39, 673, 1155, 3047, 3060
AcoI	YGGCCR	2 cut(s)	327, 1665
AcuI	CTGAAG	3 cut(s)	2226, 2822, 3143
AdeI	CACNNNGTG	1 cut(s)	1792
AfaI	GTAC	8 cut(s)	451, 1312, 1472, 1522, 1538, 2217, 2433, 2743
AfiI	CCNNNNNNNGG	4 cut(s)	409, 1490, 2266, 2799
AflIII	ACRYGT	2 cut(s)	2058, 2548
AhlI	ACTAGT	1 cut(s)	2020
AjnI	CCWGG	2 cut(s)	1807, 2853
AjuI	GAANNNNNNNTTGG	4 cut(s)	758, 790, 936, 968
AleI	CACNNNNGTG	1 cut(s)	1673
Alw21I	GWGCWC	1 cut(s)	712
Alw26I	GTCTC	4 cut(s)	386, 853, 2847, 3177
AlwI	GGATC	5 cut(s)	39, 673, 1155, 3047, 3060
AlwNI	CAGNNNCTG	1 cut(s)	2954
AoxI	GGCC	5 cut(s)	327, 1665, 1805, 2257, 2642
ApeKI	GCWGC	2 cut(s)	302, 673
AseI	ATTAAT	4 cut(s)	1197, 1230, 1959, 2424
Asp700I	GAANNNNTTC	1 cut(s)	2835
Asp718I	GGTACC	2 cut(s)	449, 2431
AspLEI	GCGC	1 cut(s)	307
AspS9I	GGNCC	2 cut(s)	318, 2521
AsuHPI	GGTGA	3 cut(s)	2317, 2705, 2739
AsuII	TTCGAA	1 cut(s)	926
AvaII	GGWCC	2 cut(s)	318, 2521
BalI	TGGCCA	2 cut(s)	329, 1667
BamHI	GGATCC	1 cut(s)	3052
BanI	GGYRCC	2 cut(s)	449, 2431
BanII	GRGCYC	2 cut(s)	274, 2710
BarI	GAAGNNNNNNTAC	4 cut(s)	442, 474, 2199, 2231
BbsI	GAAGAC	2 cut(s)	45, 77
Bbv12I	GWGCWC	1 cut(s)	712
BbvI	GCAGC	2 cut(s)	314, 685
BciT130I	CCWGG	2 cut(s)	1809, 2855
BciVI	GTATCC	1 cut(s)	2948
BcoDI	GTCTC	4 cut(s)	386, 853, 2847, 3177
BcuI	ACTAGT	1 cut(s)	2020
BfaI	CTAG	6 cut(s)	986, 1172, 2021, 2291, 2814, 3246
BfmI	CTRYAG	2 cut(s)	796, 3029
BfuI	GTATCC	1 cut(s)	2948
BglII	AGATCT	2 cut(s)	1272, 2293
BisI	GCNGC	3 cut(s)	303, 674, 2371
BlsI	GCNGC	3 cut(s)	304, 675, 2372
Bme1390I	CCNGG	2 cut(s)	1809, 2855
Bme18I	GGWCC	2 cut(s)	318, 2521
BmgT120I	GGNCC	2 cut(s)	318, 2521
BmiI	GGNNCC	4 cut(s)	320, 451, 2433, 3054
BmrFI	CCNGG	2 cut(s)	1809, 2855
BmrI	ACTGGG	1 cut(s)	987
BmsI	GCATC	7 cut(s)	289, 1447, 2071, 2218, 2380, 2485, 2590
BmuI	ACTGGG	1 cut(s)	987
BpiI	GAAGAC	2 cut(s)	45, 77
BplI	GAGNNNNNCTC	4 cut(s)	1259, 1291, 3157, 3189
BpmI	CTGGAG	5 cut(s)	1067, 1311, 1407, 1788, 2876
Bpu10I	CCTNAGC	1 cut(s)	6
Bpu14I	TTCGAA	1 cut(s)	926
BpuEI	CTTGAG	4 cut(s)	457, 1589, 2510, 2845
BsaAI	YACGTR	1 cut(s)	2474
BsaBI	GATNNNNATC	1 cut(s)	3012
BsaI	GGTCTC	2 cut(s)	386, 3177
BsaJI	CCNNGG	4 cut(s)	347, 1668, 2374, 2793
Bsc4I	CCNNNNNNNGG	4 cut(s)	409, 1490, 2266, 2799
Bse1I	ACTGG	3 cut(s)	993, 1050, 1771
Bse3DI	GCAATG	2 cut(s)	1313, 1837
Bse8I	GATNNNNATC	1 cut(s)	3012
BseBI	CCWGG	2 cut(s)	1809, 2855
BseDI	CCNNGG	4 cut(s)	347, 1668, 2374, 2793
BseGI	GGATG	8 cut(s)	150, 198, 1405, 1462, 1717, 2233, 2605, 2808
BseJI	GATNNNNATC	1 cut(s)	3012
BseLI	CCNNNNNNNGG	4 cut(s)	409, 1490, 2266, 2799
BseMI	GCAATG	2 cut(s)	1313, 1837
BseMII	CTCAG	6 cut(s)	1257, 1620, 2451, 2715, 2858, 3188
BseNI	ACTGG	3 cut(s)	993, 1050, 1771
BseRI	GAGGAG	3 cut(s)	1064, 1384, 1915
BseXI	GCAGC	2 cut(s)	314, 685
BseYI	CCCAGC	1 cut(s)	38
BsgI	GTGCAG	1 cut(s)	269
Bsh1236I	CGCG	1 cut(s)	2972
BshFI	GGCC	5 cut(s)	329, 1667, 1807, 2259, 2644
BshNI	GGYRCC	2 cut(s)	449, 2431
BsiHKAI	GWGCWC	1 cut(s)	712
BslFI	GGGAC	1 cut(s)	377
BslI	CCNNNNNNNGG	4 cut(s)	409, 1490, 2266, 2799
BsmAI	GTCTC	4 cut(s)	386, 853, 2847, 3177
BsmFI	GGGAC	1 cut(s)	377
BsmI	GAATGC	3 cut(s)	655, 779, 1485
BsnI	GGCC	5 cut(s)	329, 1667, 1807, 2259, 2644
Bso31I	GGTCTC	2 cut(s)	386, 3177
Bsp119I	TTCGAA	1 cut(s)	926
Bsp1286I	GDGCHC	3 cut(s)	274, 712, 2710
Bsp1407I	TGTACA	2 cut(s)	1470, 1520
Bsp143I	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
BspACI	CCGC	3 cut(s)	181, 1259, 2371
BspANI	GGCC	5 cut(s)	329, 1667, 1807, 2259, 2644
BspCNI	CTCAG	6 cut(s)	1258, 1619, 2450, 2716, 2857, 3187
BspFNI	CGCG	1 cut(s)	2972
BspHI	TCATGA	3 cut(s)	312, 1867, 3181
BspLI	GGNNCC	4 cut(s)	320, 451, 2433, 3054
BspPI	GGATC	5 cut(s)	39, 673, 1155, 3047, 3060
BspT104I	TTCGAA	1 cut(s)	926
BspT107I	GGYRCC	2 cut(s)	449, 2431
BspTNI	GGTCTC	2 cut(s)	386, 3177
BsrDI	GCAATG	2 cut(s)	1313, 1837
BsrGI	TGTACA	2 cut(s)	1470, 1520
BsrI	ACTGG	3 cut(s)	993, 1050, 1771
BssECI	CCNNGG	4 cut(s)	347, 1668, 2374, 2793
BssMI	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
BssNAI	GTATAC	1 cut(s)	691
BssT1I	CCWWGG	2 cut(s)	2374, 2793
Bst1107I	GTATAC	1 cut(s)	691
Bst2UI	CCWGG	2 cut(s)	1809, 2855
Bst6I	CTCTTC	4 cut(s)	29, 108, 721, 851
BstAUI	TGTACA	2 cut(s)	1470, 1520
BstBAI	YACGTR	1 cut(s)	2474
BstBI	TTCGAA	1 cut(s)	926
BstC8I	GCNNGC	2 cut(s)	2909, 2927
BstDEI	CTNAG	9 cut(s)	6, 1266, 1606, 2316, 2437, 2639, 2724, 2844, 3174
BstDSI	CCRYGG	1 cut(s)	1668
BstENI	CCTNNNNNAGG	1 cut(s)	2264
BstF5I	GGATG	8 cut(s)	150, 198, 1405, 1462, 1717, 2233, 2605, 2808
BstFNI	CGCG	1 cut(s)	2972
BstHHI	GCGC	1 cut(s)	307
BstKTI	GATC	7 cut(s)	47, 668, 1150, 1275, 2106, 2296, 3055
BstMAI	GTCTC	4 cut(s)	386, 853, 2847, 3177
BstMBI	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
BstMWI	GCNNNNNNNGC	2 cut(s)	1645, 1984
BstNI	CCWGG	2 cut(s)	1809, 2855
BstNSI	RCATGY	5 cut(s)	361, 1325, 1617, 2062, 2552
BstSCI	CCNGG	2 cut(s)	1807, 2853
BstSFI	CTRYAG	2 cut(s)	796, 3029
BstSNI	TACGTA	1 cut(s)	2474
BstUI	CGCG	1 cut(s)	2972
BstV1I	GCAGC	2 cut(s)	314, 685
BstV2I	GAAGAC	2 cut(s)	45, 77
BstX2I	RGATCY	5 cut(s)	665, 1147, 1272, 2293, 3052
BstXI	CCANNNNNNTGG	1 cut(s)	1021
BstYI	RGATCY	5 cut(s)	665, 1147, 1272, 2293, 3052
BstZ17I	GTATAC	1 cut(s)	691
BsuI	GTATCC	1 cut(s)	2948
BsuRI	GGCC	5 cut(s)	329, 1667, 1807, 2259, 2644
BtgI	CCRYGG	1 cut(s)	1668
BtsCI	GGATG	8 cut(s)	150, 198, 1405, 1462, 1717, 2233, 2605, 2808
BtsI	GCAGTG	2 cut(s)	1899, 2955
BtsIMutI	CAGTG	8 cut(s)	597, 1899, 2287, 2655, 2721, 2918, 2955, 3215
Cac8I	GCNNGC	2 cut(s)	2909, 2927
CaiI	CAGNNNCTG	1 cut(s)	2954
CciI	TCATGA	3 cut(s)	312, 1867, 3181
CfoI	GCGC	1 cut(s)	307
Cfr13I	GGNCC	2 cut(s)	318, 2521
CseI	GACGC	1 cut(s)	2961
Csp6I	GTAC	8 cut(s)	450, 1311, 1471, 1521, 1537, 2216, 2432, 2742
CviQI	GTAC	8 cut(s)	450, 1311, 1471, 1521, 1537, 2216, 2432, 2742
DdeI	CTNAG	9 cut(s)	6, 1266, 1606, 2316, 2437, 2639, 2724, 2844, 3174
DpnI	GATC	7 cut(s)	46, 667, 1149, 1274, 2105, 2295, 3054
DpnII	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
DraI	TTTAAA	4 cut(s)	1750, 1776, 1995, 2536
DraIII	CACNNNGTG	1 cut(s)	1792
EaeI	YGGCCR	2 cut(s)	327, 1665
Eam1104I	CTCTTC	4 cut(s)	29, 108, 721, 851
EarI	CTCTTC	4 cut(s)	29, 108, 721, 851
Eco105I	TACGTA	1 cut(s)	2474
Eco130I	CCWWGG	2 cut(s)	2374, 2793
Eco147I	AGGCCT	1 cut(s)	2644
Eco24I	GRGCYC	2 cut(s)	274, 2710
Eco31I	GGTCTC	2 cut(s)	386, 3177
Eco47I	GGWCC	2 cut(s)	318, 2521
Eco57I	CTGAAG	3 cut(s)	2226, 2822, 3143
EcoNI	CCTNNNNNAGG	1 cut(s)	2264
EcoO109I	RGGNCCY	1 cut(s)	318
EcoRI	GAATTC	1 cut(s)	2651
EcoRII	CCWGG	2 cut(s)	1807, 2853
EcoT14I	CCWWGG	2 cut(s)	2374, 2793
EcoT22I	ATGCAT	2 cut(s)	2233, 2395
EcoT38I	GRGCYC	2 cut(s)	274, 2710
ErhI	CCWWGG	2 cut(s)	2374, 2793
FalI	AAGNNNNNCTT	4 cut(s)	1690, 1722, 2367, 2399
FaqI	GGGAC	1 cut(s)	377
FauI	CCCGC	1 cut(s)	188
FauNDI	CATATG	1 cut(s)	2592
FblI	GTMKAC	1 cut(s)	690
Fnu4HI	GCNGC	3 cut(s)	303, 674, 2371
FokI	GGATG	8 cut(s)	137, 185, 1412, 1469, 1724, 2240, 2612, 2815
FriOI	GRGCYC	2 cut(s)	274, 2710
Fsp4HI	GCNGC	3 cut(s)	303, 674, 2371
FspBI	CTAG	6 cut(s)	986, 1172, 2021, 2291, 2814, 3246
GlaI	GCGC	1 cut(s)	306
GluI	GCNGC	3 cut(s)	303, 674, 2371
GsaI	CCCAGC	1 cut(s)	42
GsuI	CTGGAG	5 cut(s)	1067, 1311, 1407, 1788, 2876
HaeIII	GGCC	5 cut(s)	329, 1667, 1807, 2259, 2644
HgaI	GACGC	1 cut(s)	2961
HhaI	GCGC	1 cut(s)	307
Hin6I	GCGC	1 cut(s)	305
HinP1I	GCGC	1 cut(s)	305
HindIII	AAGCTT	6 cut(s)	8, 333, 929, 1699, 2609, 2905
HphI	GGTGA	3 cut(s)	2317, 2705, 2739
Hpy166II	GTNNAC	3 cut(s)	691, 1786, 2216
Hpy188I	TCNGA	9 cut(s)	348, 826, 852, 1589, 1695, 1956, 2440, 2994, 3123
Hpy188III	TCNNGA	9 cut(s)	313, 663, 1151, 1290, 1868, 2149, 2225, 2824, 3182
Hpy8I	GTNNAC	3 cut(s)	691, 1786, 2216
HpyCH4IV	ACGT	2 cut(s)	121, 2473
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1645, 1984
HpyF3I	CTNAG	9 cut(s)	6, 1266, 1606, 2316, 2437, 2639, 2724, 2844, 3174
HpySE526I	ACGT	2 cut(s)	121, 2473
HspAI	GCGC	1 cut(s)	305
KpnI	GGTACC	2 cut(s)	453, 2435
Kzo9I	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
LmnI	GCTCC	2 cut(s)	2857, 3232
Lsp1109I	GCAGC	2 cut(s)	314, 685
LweI	GCATC	7 cut(s)	289, 1447, 2071, 2218, 2380, 2485, 2590
MaeI	CTAG	6 cut(s)	986, 1172, 2021, 2291, 2814, 3246
MaeII	ACGT	2 cut(s)	121, 2473
MaeIII	GTNAC	7 cut(s)	77, 1210, 2659, 2950, 3041, 3064, 3079
MalI	GATC	7 cut(s)	46, 667, 1149, 1274, 2105, 2295, 3054
MboI	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
MfeI	CAATTG	2 cut(s)	522, 951
MflI	RGATCY	5 cut(s)	665, 1147, 1272, 2293, 3052
MhlI	GDGCHC	3 cut(s)	274, 712, 2710
MlsI	TGGCCA	2 cut(s)	329, 1667
MluNI	TGGCCA	2 cut(s)	329, 1667
MlyI	GAGTC	5 cut(s)	230, 841, 1398, 1880, 2903
MmeI	TCCRAC	1 cut(s)	785
Mox20I	TGGCCA	2 cut(s)	329, 1667
Mph1103I	ATGCAT	2 cut(s)	2233, 2395
MroXI	GAANNNNTTC	1 cut(s)	2835
MscI	TGGCCA	2 cut(s)	329, 1667
MslI	CAYNNNNRTG	3 cut(s)	291, 1016, 1673
Msp20I	TGGCCA	2 cut(s)	329, 1667
MspR9I	CCNGG	2 cut(s)	1809, 2855
MssI	GTTTAAAC	1 cut(s)	2536
MunI	CAATTG	2 cut(s)	522, 951
Mva1269I	GAATGC	3 cut(s)	655, 779, 1485
MvaI	CCWGG	2 cut(s)	1809, 2855
MvnI	CGCG	1 cut(s)	2972
MwoI	GCNNNNNNNGC	2 cut(s)	1645, 1984
NdeI	CATATG	1 cut(s)	2592
NdeII	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
NlaIV	GGNNCC	4 cut(s)	320, 451, 2433, 3054
NmuCI	GTSAC	2 cut(s)	2950, 3041
NsiI	ATGCAT	2 cut(s)	2233, 2395
NspI	RCATGY	5 cut(s)	361, 1325, 1617, 2062, 2552
NspV	TTCGAA	1 cut(s)	926
OliI	CACNNNNGTG	1 cut(s)	1673
PagI	TCATGA	3 cut(s)	312, 1867, 3181
PceI	AGGCCT	1 cut(s)	2644
PciI	ACATGT	2 cut(s)	2058, 2548
PcsI	WCGNNNNNNNCGW	1 cut(s)	1919
PctI	GAATGC	3 cut(s)	655, 779, 1485
PdmI	GAANNNNTTC	1 cut(s)	2835
PfeI	GAWTC	8 cut(s)	186, 352, 1026, 2044, 2322, 2408, 2465, 3098
PfoI	TCCNGGA	1 cut(s)	2853
PkrI	GCNGC	3 cut(s)	304, 675, 2372
PleI	GAGTC	5 cut(s)	229, 840, 1397, 1879, 2902
PmeI	GTTTAAAC	1 cut(s)	2536
PpsI	GAGTC	5 cut(s)	229, 840, 1397, 1879, 2902
Ppu21I	YACGTR	1 cut(s)	2474
PpuMI	RGGWCCY	1 cut(s)	318
PscI	ACATGT	2 cut(s)	2058, 2548
PshBI	ATTAAT	4 cut(s)	1197, 1230, 1959, 2424
PsiI	TTATAA	3 cut(s)	1620, 2346, 2357
Psp5II	RGGWCCY	1 cut(s)	318
Psp6I	CCWGG	2 cut(s)	1807, 2853
PspFI	CCCAGC	1 cut(s)	38
PspGI	CCWGG	2 cut(s)	1807, 2853
PspN4I	GGNNCC	4 cut(s)	320, 451, 2433, 3054
PspPI	GGNCC	2 cut(s)	318, 2521
PspPPI	RGGWCCY	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	2954
PsuI	RGATCY	5 cut(s)	665, 1147, 1272, 2293, 3052
RsaI	GTAC	8 cut(s)	451, 1312, 1472, 1522, 1538, 2217, 2433, 2743
RsaNI	GTAC	8 cut(s)	450, 1311, 1471, 1521, 1537, 2216, 2432, 2742
RseI	CAYNNNNRTG	3 cut(s)	291, 1016, 1673
SatI	GCNGC	3 cut(s)	303, 674, 2371
Sau3AI	GATC	7 cut(s)	44, 665, 1147, 1272, 2103, 2293, 3052
Sau96I	GGNCC	2 cut(s)	318, 2521
SchI	GAGTC	5 cut(s)	230, 841, 1398, 1880, 2903
ScrFI	CCNGG	2 cut(s)	1809, 2855
SduI	GDGCHC	3 cut(s)	274, 712, 2710
SfaNI	GCATC	7 cut(s)	289, 1447, 2071, 2218, 2380, 2485, 2590
SfcI	CTRYAG	2 cut(s)	796, 3029
SfuI	TTCGAA	1 cut(s)	926
SinI	GGWCC	2 cut(s)	318, 2521
SmiMI	CAYNNNNRTG	3 cut(s)	291, 1016, 1673
SmlI	CTYRAG	4 cut(s)	436, 1568, 2525, 2824
SmoI	CTYRAG	4 cut(s)	436, 1568, 2525, 2824
SnaBI	TACGTA	1 cut(s)	2474
SpeI	ACTAGT	1 cut(s)	2020
SseBI	AGGCCT	1 cut(s)	2644
SsiI	CCGC	3 cut(s)	181, 1259, 2371
SspI	AATATT	1 cut(s)	2335
SspMI	CTAG	6 cut(s)	986, 1172, 2021, 2291, 2814, 3246
StuI	AGGCCT	1 cut(s)	2644
StyD4I	CCNGG	2 cut(s)	1807, 2853
StyI	CCWWGG	2 cut(s)	2374, 2793
TaiI	ACGT	2 cut(s)	124, 2476
TaqI	TCGA	5 cut(s)	224, 783, 926, 1077, 3224
TatI	WGTACW	3 cut(s)	1310, 1470, 1520
TauI	GCSGC	1 cut(s)	2373
TfiI	GAWTC	8 cut(s)	186, 352, 1026, 2044, 2322, 2408, 2465, 3098
TscAI	CASTG	8 cut(s)	597, 1899, 2287, 2662, 2728, 2925, 2955, 3222
TseFI	GTSAC	2 cut(s)	2950, 3041
TseI	GCWGC	2 cut(s)	302, 673
Tsp45I	GTSAC	2 cut(s)	2950, 3041
TspGWI	ACGGA	2 cut(s)	1685, 2906
TspRI	CASTG	8 cut(s)	597, 1899, 2287, 2662, 2728, 2925, 2955, 3222
VpaK11BI	GGWCC	2 cut(s)	318, 2521
VspI	ATTAAT	4 cut(s)	1197, 1230, 1959, 2424
XagI	CCTNNNNNAGG	1 cut(s)	2264
XceI	RCATGY	5 cut(s)	361, 1325, 1617, 2062, 2552
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1018, 1372, 2800
XmiI	GTMKAC	1 cut(s)	690
XmnI	GAANNNNTTC	1 cut(s)	2835
XspI	CTAG	6 cut(s)	986, 1172, 2021, 2291, 2814, 3246
Zsp2I	ATGCAT	2 cut(s)	2233, 2395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.