RchiOBHm_Chr4g0399881

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008661
Physical Location & Seq
Forward (+)
17111331 .. 17118345
7015 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37198

Sequence Viewer

Length: 3288 bp
ATGCCTAAGCTTTCCTCTACACTCTTCTACACCAAACCCAGCCGAGCCTTGTCTTCATCTTCTTCTTCTCCTCGTAGCAGTCTTCCAAAACTCCCACCTCTTCAAACTTTACTCAAACGTGGCTTCACTCCCACTCTCAATTCCATCATCCAATTCCTTCTCTTTCTTTCACATACCCACAGATTCAACACAGTCATCCACTTCTTCTCTCAAATGGAGTCGAACCAAATCAAAGGCAACTCCCAAACTCGCTCAATTCTCACATGGGCTCTCCTTAAACTGCACAAATATGAAGATGCAGAGCATTTCATGAGGACCCAGATGGCCAAAGCTTCAAATTTTCCGAGGAATCGCATGTGGGACACTCTCATTCAAGGTCTCTGTATCAACCAGAAAGACCCAGATAAGGCTTTGCTGGTATTACGGGATTGCTTGAGAAAATATGGTACCTTTCCTTCTTCTTTCACTTTTTGCTCGTTAATTTATAGATTTAGCTCTATGGGGGATATGAGTAAGGCAATTGAGGTTGTGGAGTTGATGACAGATGAGAAAATCAACTACCCATTTAACAATTTTGTTTGTAGTTCAGTGATTTCTGGGTTTTGTAAAATTGGAAAGCCAGAAATTGCTGCTGAGTTTTTTGAGAATGCCCTAAAGTCTGGAGCTTTGCAGCCTAATGTTGTGGTATACACAGCACTTGTAGGTGCTCTTTGTAAATTAGGAAGAGTTAGTGAGGTTTGTGATTTAGTTTGTAAAATGGCGAAGGAAGGATTGGCATTCGATGTTGTTTTCTATAGTAGTTGGATTTGTGGTTATATTTCAGAAGGGAGTCTAATGGAGGTGTTTCGGAAGAAGAGACAGATGGTAGATAAAGGCATAAGACCAGATACAGTTAGCTATACTATACTTGTAGATGGGTTTTCGAAGCTTGGAGATGTGGAAAAGGCAATTGGCTTTTTGAAAAAGATGAGGAAAGGTGGGCTAGAACCCAGTTTGATTACTTATACCACCATCATGTTGGGATTCTGCGAGAAAGGAAAACTGGAGGAGGCATTTGCTATTTACAAGATGGTCAAAGATTTGGAAATTGAAGTTGATGAATTTATGTATGCTACTTTAATTCACGGGTTTTGTATGAGAGGGGATCTTGATGGTGTTTTTCGTCTGCTAGATGAAATGGAAAAGAAGGGGATTAATCCAAGCATTGTTACATATAATACGGTAATTAATGGATTATGCAAATTTGGGAGGACTGCGGAGGCTGAGAAGATCTCAAAGGGCATATCTGGAGATACCATTACATATAGTACATTGCTACATGGGTATATTGAGGAAGAAAATACCTCTGGGATTTTGGAAACCAAGAGGAGATTGGAGGAAGCTGGAGTCTTTATGGATGTTGTTATGTGTAATATACTTATTAAAGCACTATTCATGGTGGGGGCATTTGAGGATGCTTACATACTGTACAAGGGAATGCCAGAGAAGGGTTTGACTGCAAATTCCATTACATACTGTACAATGATTGATGGGTACTGTAAAGTTGGTAGAATTGATGAGGCACTTGAGATATTTGATGAGTTCAGAAGGACACCACTTTCCTCAGTTGCATGTTATAATTGTATAATAAATTGGCTCTGTAAGCAGGGCATGGTAGATATGGCCACAGAGGTGTTTATTGAACTTAATCAGAAAAGCTTAACTTTGGATGATGGTATCTGCAAAATGCTTTTGAAGGCAACTTTTAAACAAAAAAGTGCAACTGGAGTTTTAAATTTGGTTCACAGAGTGGAAAATTTGAGGCCAGGTATGTATGATGTTATGAGCAATGTTGCGATCAGTTTCTTGTGCAAGAGAGGGTTTCATGAGTCTGCATTTGAAGTGTATGCAGTGATGAGGAGGGAAGGGTCGGTTGCAACGAGAAAAACTTATTATTCAATTTTAGATGGTCTAATTAATGATGGAAAAGAGTGGCTAACTCTGCCTCTTTTAAACATCTTTCTAAAAGAATATGGACTAGTTGAACCAAAAGTAAGCCAGATTCTTGCTTACCACATGTGCCTGAAGGATGTCAATGATGCTTTCTGCTTTTTAGAAAAGATCAAGGACAGACCTACAGCCATTACCTTGCCTATTTCTCTTTTCAAGACACTTATGAAGAAGGGTAAAGTTTTGGTTGCGTATCAGCTTTTCATGGAGGCTGAAGATAGTGTACCCGTTCTGGATGCATTTGATTATTCGCACATGGTTGATGGCCTTTGCAAGGGTGGATATATCAGTGAGGCACTAGATCTCTGTGGTTTTGCTAAATCTAAGGGAATCACCCTGAATATTATTGCTTATAATTCAGTTATAAATGGATTGTGCCGCCAAGGTCATCTTGTTGATGCATTTCGGCTATTTGATTCATTAGAGAGAATTAATTTGGTACCCTCAGAAATCACTTATGCTACATTGATTGATTCCCTACGTAGAGAGGGGTTTCTACTTGATGCAAAGCAATTATTTGAAAGCATGGTCCTCAAGGGGTTTAAACCGAATACACATGTATATAACTCTATTATTGATGGTTTTTGCAAAATTGGACATATGGAGGATGCCTTGAAGCTTCTTTGTGAATTGGACATGAAATCTCTTAGGCCTGATGAATTCACTGTTACTATTGTTATCAATGGCTTTTGCCAAAAGGGTGATATGGAAGGGGCTCTAAATTTCTTCACTGAGTTCAAAAGAAATGGTACATCACCTGATTTTTTGGGTTTCTTAAATTTGATAAGAGGTTTATGTGCCAAGGGAAGGATGGAAGAAGCCAGAAGTATCTTGAGAGAAATGCTTCAGTCTCAGTCTGTCCTGGAGCTAATAAATAGAGTTGATGTTGAGCTGGAAACAGAGTCATTGGAAAGCTTGCTTGTTTCACTGTGCGAGCAAGGAAGTATCCAAGAAGCAGTGACTGTCCTTAATGAAATCGCGTCCATGTTTTTCCCTGTTCGGAGTTCATCTATTGATCGCCATCAATCTCATAAACTACAGAAGCCTTGTGACAGCGAGGATCCTAAAACAGTTGCTTCAAAATCTGTAACTTCCATCAATGCAGATTCGGGTATTCAGTTTTCTGGTCGGAAGAAAGTAGAGAAGGTGGCAAAAACTTGTGATGTTATAGGGAGTAGGTCTCAGTTTCATGACTTTGATTATTACTATAAGCAAATTGCTTCACTGTGTTCGATAGGGGAGCTACAAAAGGCTAGTGAATTAGTAAAGGAAATAGTTTCTAATTTGGGCAAGAGTTGA

Protein Analysis

1095

Amino Acids

123.09

Weight (kDa)

7.16

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S27 PF10037 127 - 189 2.4e-06 Mitochondrial 28S ribosomal protein S27
PPR_2 PF13041 225 - 266 3e-06 PPR repeat family
PPR_2 PF13041 261 - 309 9.4e-09 PPR repeat family
PPR_long PF17177 277 - 374 5.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 285 - 339 1.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 291 - 324 1.6e-08 PPR repeat
PPR PF01535 298 - 327 1.7e-06 PPR repeat
PPR_2 PF13041 305 - 343 3.1e-08 PPR repeat family
PPR_1 PF12854 327 - 354 8.4e-07 PPR repeat
PPR_2 PF13041 330 - 378 1.6e-09 PPR repeat family
TPR_24 PF23276 351 - 491 3.7e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 356 - 410 1.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 363 - 394 2.4e-07 PPR repeat
PPR_2 PF13041 366 - 414 5e-16 PPR repeat family
PPR PF01535 369 - 398 7.7e-06 PPR repeat
PPR_1 PF12854 396 - 423 1.1e-09 PPR repeat
PPR_2 PF13041 466 - 514 9.2e-13 PPR repeat family
PPR_1 PF12854 496 - 527 1.9e-11 PPR repeat
PPR_2 PF13041 501 - 529 7e-06 PPR repeat family
PPR PF01535 503 - 530 3.7e-08 PPR repeat
PPR_2 PF13041 535 - 570 1.7e-06 PPR repeat family
PPR_2 PF13041 746 - 790 7.3e-07 PPR repeat family
PPR_1 PF12854 772 - 803 2e-09 PPR repeat
PPR_2 PF13041 777 - 823 5.4e-12 PPR repeat family
PPR_2 PF13041 811 - 860 2.1e-10 PPR repeat family
PPR_1 PF12854 842 - 873 5.5e-10 PPR repeat
PPR_2 PF13041 846 - 895 1.5e-15 PPR repeat family
PPR_1 PF12854 878 - 908 6.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1617, 2343, 2354
Acc65I GGTACC 2 cut(s) 446, 2428
AccB1I GGYRCC 2 cut(s) 446, 2428
AccI GTMKAC 1 cut(s) 687
AccII CGCG 1 cut(s) 2969
AciI CCGC 2 cut(s) 1256, 2368
AclWI GGATC 3 cut(s) 1152, 3044, 3057
AcoI YGGCCR 2 cut(s) 324, 1662
AcsI RAATTY 9 cut(s) 337, 1100, 1241, 1501, 1774, 1795, 2648, 2710, 2767
AcuI CTGAAG 3 cut(s) 2084, 2223, 2819
AdeI CACNNNGTG 1 cut(s) 1789
AfaI GTAC 8 cut(s) 448, 1309, 1469, 1519, 1535, 2214, 2430, 2740
AfiI CCNNNNNNNGG 4 cut(s) 406, 1487, 2263, 2796
AflIII ACRYGT 2 cut(s) 2055, 2545
AhlI ACTAGT 1 cut(s) 2017
AjnI CCWGG 2 cut(s) 1804, 2850
AjuI GAANNNNNNNTTGG 4 cut(s) 755, 787, 933, 965
AleI CACNNNNGTG 1 cut(s) 1670
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 4 cut(s) 383, 850, 2844, 3174
AlwI GGATC 3 cut(s) 1152, 3044, 3057
AlwNI CAGNNNCTG 1 cut(s) 2951
AoxI GGCC 5 cut(s) 324, 1662, 1802, 2254, 2639
ApeKI GCWGC 2 cut(s) 629, 670
ApoI RAATTY 9 cut(s) 337, 1100, 1241, 1501, 1774, 1795, 2648, 2710, 2767
AseI ATTAAT 4 cut(s) 1194, 1227, 1956, 2421
Asp700I GAANNNNTTC 1 cut(s) 2832
Asp718I GGTACC 2 cut(s) 446, 2428
AspS9I GGNCC 2 cut(s) 315, 2518
AsuHPI GGTGA 3 cut(s) 2314, 2702, 2736
AsuII TTCGAA 1 cut(s) 923
AvaII GGWCC 2 cut(s) 315, 2518
BalI TGGCCA 2 cut(s) 326, 1664
BamHI GGATCC 1 cut(s) 3049
BanI GGYRCC 2 cut(s) 446, 2428
BanII GRGCYC 2 cut(s) 271, 2707
BarI GAAGNNNNNNTAC 4 cut(s) 439, 471, 2196, 2228
BbsI GAAGAC 2 cut(s) 45, 74
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 2 cut(s) 616, 682
BciT130I CCWGG 2 cut(s) 1806, 2852
BciVI GTATCC 1 cut(s) 2945
BcoDI GTCTC 4 cut(s) 383, 850, 2844, 3174
BcuI ACTAGT 1 cut(s) 2017
BfaI CTAG 5 cut(s) 983, 1169, 2018, 2288, 3243
BfmI CTRYAG 3 cut(s) 793, 2115, 3026
BfuI GTATCC 1 cut(s) 2945
BglII AGATCT 2 cut(s) 1269, 2290
BisI GCNGC 3 cut(s) 630, 671, 2368
BlsI GCNGC 3 cut(s) 631, 672, 2369
Bme1390I CCNGG 2 cut(s) 1806, 2852
Bme18I GGWCC 2 cut(s) 315, 2518
BmgT120I GGNCC 2 cut(s) 315, 2518
BmiI GGNNCC 4 cut(s) 317, 448, 2430, 3051
BmrFI CCNGG 2 cut(s) 1806, 2852
BmrI ACTGGG 1 cut(s) 984
BmsI GCATC 7 cut(s) 286, 1444, 2068, 2215, 2377, 2482, 2587
BmuI ACTGGG 1 cut(s) 984
BpiI GAAGAC 2 cut(s) 45, 74
BplI GAGNNNNNCTC 4 cut(s) 1256, 1288, 3154, 3186
BpmI CTGGAG 6 cut(s) 681, 1064, 1308, 1404, 1785, 2873
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 923
BpuEI CTTGAG 4 cut(s) 454, 1586, 2507, 2842
BsaAI YACGTR 1 cut(s) 2471
BsaBI GATNNNNATC 1 cut(s) 3009
BsaI GGTCTC 2 cut(s) 383, 3174
BsaJI CCNNGG 3 cut(s) 344, 2371, 2790
Bsc4I CCNNNNNNNGG 4 cut(s) 406, 1487, 2263, 2796
Bse1I ACTGG 3 cut(s) 990, 1047, 1768
Bse3DI GCAATG 2 cut(s) 1310, 1834
Bse8I GATNNNNATC 1 cut(s) 3009
BseBI CCWGG 2 cut(s) 1806, 2852
BseDI CCNNGG 3 cut(s) 344, 2371, 2790
BseGI GGATG 9 cut(s) 147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2805
BseJI GATNNNNATC 1 cut(s) 3009
BseLI CCNNNNNNNGG 4 cut(s) 406, 1487, 2263, 2796
BseMI GCAATG 2 cut(s) 1310, 1834
BseMII CTCAG 7 cut(s) 624, 1254, 1617, 2448, 2712, 2855, 3185
BseNI ACTGG 3 cut(s) 990, 1047, 1768
BseRI GAGGAG 4 cut(s) 60, 1061, 1381, 1912
BseXI GCAGC 2 cut(s) 616, 682
BseYI CCCAGC 1 cut(s) 38
BsgI GTGCAG 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 2969
BshFI GGCC 5 cut(s) 326, 1664, 1804, 2256, 2641
BshNI GGYRCC 2 cut(s) 446, 2428
BsiHKAI GWGCWC 1 cut(s) 709
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 4 cut(s) 406, 1487, 2263, 2796
BsmAI GTCTC 4 cut(s) 383, 850, 2844, 3174
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 3 cut(s) 652, 776, 1482
BsnI GGCC 5 cut(s) 326, 1664, 1804, 2256, 2641
Bso31I GGTCTC 2 cut(s) 383, 3174
Bsp119I TTCGAA 1 cut(s) 923
Bsp1286I GDGCHC 3 cut(s) 271, 709, 2707
Bsp1407I TGTACA 2 cut(s) 1467, 1517
Bsp143I GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
BspACI CCGC 2 cut(s) 1256, 2368
BspANI GGCC 5 cut(s) 326, 1664, 1804, 2256, 2641
BspCNI CTCAG 7 cut(s) 625, 1255, 1616, 2447, 2713, 2854, 3184
BspFNI CGCG 1 cut(s) 2969
BspHI TCATGA 3 cut(s) 309, 1864, 3178
BspLI GGNNCC 4 cut(s) 317, 448, 2430, 3051
BspPI GGATC 3 cut(s) 1152, 3044, 3057
BspT104I TTCGAA 1 cut(s) 923
BspT107I GGYRCC 2 cut(s) 446, 2428
BspTNI GGTCTC 2 cut(s) 383, 3174
BsrDI GCAATG 2 cut(s) 1310, 1834
BsrGI TGTACA 2 cut(s) 1467, 1517
BsrI ACTGG 3 cut(s) 990, 1047, 1768
BssECI CCNNGG 3 cut(s) 344, 2371, 2790
BssMI GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
BssNAI GTATAC 1 cut(s) 688
BssT1I CCWWGG 2 cut(s) 2371, 2790
Bst1107I GTATAC 1 cut(s) 688
Bst2UI CCWGG 2 cut(s) 1806, 2852
Bst6I CTCTTC 4 cut(s) 29, 105, 718, 848
BstAUI TGTACA 2 cut(s) 1467, 1517
BstBAI YACGTR 1 cut(s) 2471
BstBI TTCGAA 1 cut(s) 923
BstC8I GCNNGC 2 cut(s) 2906, 2924
BstENI CCTNNNNNAGG 1 cut(s) 2261
BstF5I GGATG 9 cut(s) 147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2805
BstFNI CGCG 1 cut(s) 2969
BstKTI GATC 7 cut(s) 1147, 1272, 1839, 2103, 2293, 3007, 3052
BstMAI GTCTC 4 cut(s) 383, 850, 2844, 3174
BstMBI GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
BstMWI GCNNNNNNNGC 2 cut(s) 1642, 1981
BstNI CCWGG 2 cut(s) 1806, 2852
BstNSI RCATGY 4 cut(s) 358, 1614, 2059, 2549
BstSCI CCNGG 2 cut(s) 1804, 2850
BstSFI CTRYAG 3 cut(s) 793, 2115, 3026
BstSNI TACGTA 1 cut(s) 2471
BstUI CGCG 1 cut(s) 2969
BstV1I GCAGC 2 cut(s) 616, 682
BstV2I GAAGAC 2 cut(s) 45, 74
BstX2I RGATCY 4 cut(s) 1144, 1269, 2290, 3049
BstXI CCANNNNNNTGG 1 cut(s) 1018
BstYI RGATCY 4 cut(s) 1144, 1269, 2290, 3049
BstZ17I GTATAC 1 cut(s) 688
BsuI GTATCC 1 cut(s) 2945
BsuRI GGCC 5 cut(s) 326, 1664, 1804, 2256, 2641
BtsCI GGATG 9 cut(s) 147, 195, 1402, 1459, 1714, 2074, 2230, 2602, 2805
BtsI GCAGTG 2 cut(s) 1896, 2952
BtsIMutI CAGTG 8 cut(s) 594, 1896, 2284, 2652, 2718, 2915, 2952, 3212
Cac8I GCNNGC 2 cut(s) 2906, 2924
CaiI CAGNNNCTG 1 cut(s) 2951
CciI TCATGA 3 cut(s) 309, 1864, 3178
Cfr13I GGNCC 2 cut(s) 315, 2518
CseI GACGC 1 cut(s) 2958
Csp6I GTAC 8 cut(s) 447, 1308, 1468, 1518, 1534, 2213, 2429, 2739
CviQI GTAC 8 cut(s) 447, 1308, 1468, 1518, 1534, 2213, 2429, 2739
DpnI GATC 7 cut(s) 1146, 1271, 1838, 2102, 2292, 3006, 3051
DpnII GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
DraI TTTAAA 4 cut(s) 1747, 1773, 1992, 2533
DraIII CACNNNGTG 1 cut(s) 1789
EaeI YGGCCR 2 cut(s) 324, 1662
Eam1104I CTCTTC 4 cut(s) 29, 105, 718, 848
EarI CTCTTC 4 cut(s) 29, 105, 718, 848
Eco105I TACGTA 1 cut(s) 2471
Eco130I CCWWGG 2 cut(s) 2371, 2790
Eco147I AGGCCT 1 cut(s) 2641
Eco24I GRGCYC 2 cut(s) 271, 2707
Eco31I GGTCTC 2 cut(s) 383, 3174
Eco47I GGWCC 2 cut(s) 315, 2518
Eco57I CTGAAG 3 cut(s) 2084, 2223, 2819
EcoNI CCTNNNNNAGG 1 cut(s) 2261
EcoO109I RGGNCCY 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 2648
EcoRII CCWGG 2 cut(s) 1804, 2850
EcoT14I CCWWGG 2 cut(s) 2371, 2790
EcoT22I ATGCAT 2 cut(s) 2230, 2392
EcoT38I GRGCYC 2 cut(s) 271, 2707
ErhI CCWWGG 2 cut(s) 2371, 2790
FalI AAGNNNNNCTT 4 cut(s) 1687, 1719, 2364, 2396
FaqI GGGAC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 2589
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 630, 671, 2368
FokI GGATG 9 cut(s) 134, 182, 1409, 1466, 1721, 2081, 2237, 2609, 2812
FriOI GRGCYC 2 cut(s) 271, 2707
Fsp4HI GCNGC 3 cut(s) 630, 671, 2368
FspBI CTAG 5 cut(s) 983, 1169, 2018, 2288, 3243
GluI GCNGC 3 cut(s) 630, 671, 2368
GsaI CCCAGC 1 cut(s) 42
GsuI CTGGAG 6 cut(s) 681, 1064, 1308, 1404, 1785, 2873
HaeIII GGCC 5 cut(s) 326, 1664, 1804, 2256, 2641
HgaI GACGC 1 cut(s) 2958
HindIII AAGCTT 6 cut(s) 8, 330, 926, 1696, 2606, 2902
HphI GGTGA 3 cut(s) 2314, 2702, 2736
Hpy166II GTNNAC 3 cut(s) 688, 1783, 2213
Hpy188I TCNGA 8 cut(s) 345, 823, 849, 1586, 1692, 2437, 2991, 3120
Hpy188III TCNNGA 9 cut(s) 310, 660, 1148, 1287, 1865, 2146, 2222, 2821, 3179
Hpy8I GTNNAC 3 cut(s) 688, 1783, 2213
HpyCH4IV ACGT 2 cut(s) 118, 2470
HpyF10VI GCNNNNNNNGC 2 cut(s) 1642, 1981
HpySE526I ACGT 2 cut(s) 118, 2470
KpnI GGTACC 2 cut(s) 450, 2432
Kzo9I GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
LmnI GCTCC 3 cut(s) 662, 2854, 3229
Lsp1109I GCAGC 2 cut(s) 616, 682
LweI GCATC 7 cut(s) 286, 1444, 2068, 2215, 2377, 2482, 2587
MaeI CTAG 5 cut(s) 983, 1169, 2018, 2288, 3243
MaeII ACGT 2 cut(s) 118, 2470
MaeIII GTNAC 5 cut(s) 1207, 2656, 2947, 3038, 3076
MalI GATC 7 cut(s) 1146, 1271, 1838, 2102, 2292, 3006, 3051
MboI GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
MfeI CAATTG 2 cut(s) 519, 948
MflI RGATCY 4 cut(s) 1144, 1269, 2290, 3049
MhlI GDGCHC 3 cut(s) 271, 709, 2707
MlsI TGGCCA 2 cut(s) 326, 1664
MluNI TGGCCA 2 cut(s) 326, 1664
MlyI GAGTC 5 cut(s) 227, 838, 1395, 1877, 2900
MmeI TCCRAC 2 cut(s) 782, 3098
Mox20I TGGCCA 2 cut(s) 326, 1664
Mph1103I ATGCAT 2 cut(s) 2230, 2392
MroXI GAANNNNTTC 1 cut(s) 2832
MscI TGGCCA 2 cut(s) 326, 1664
MslI CAYNNNNRTG 3 cut(s) 288, 1013, 1670
Msp20I TGGCCA 2 cut(s) 326, 1664
MspR9I CCNGG 2 cut(s) 1806, 2852
MssI GTTTAAAC 1 cut(s) 2533
MunI CAATTG 2 cut(s) 519, 948
Mva1269I GAATGC 3 cut(s) 652, 776, 1482
MvaI CCWGG 2 cut(s) 1806, 2852
MvnI CGCG 1 cut(s) 2969
MwoI GCNNNNNNNGC 2 cut(s) 1642, 1981
NdeI CATATG 1 cut(s) 2589
NdeII GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
NlaIV GGNNCC 4 cut(s) 317, 448, 2430, 3051
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 2 cut(s) 2947, 3038
NsiI ATGCAT 2 cut(s) 2230, 2392
NspI RCATGY 4 cut(s) 358, 1614, 2059, 2549
NspV TTCGAA 1 cut(s) 923
OliI CACNNNNGTG 1 cut(s) 1670
PagI TCATGA 3 cut(s) 309, 1864, 3178
PceI AGGCCT 1 cut(s) 2641
PciI ACATGT 2 cut(s) 2055, 2545
PcsI WCGNNNNNNNCGW 1 cut(s) 1916
PctI GAATGC 3 cut(s) 652, 776, 1482
PdmI GAANNNNTTC 1 cut(s) 2832
PfeI GAWTC 8 cut(s) 183, 349, 1023, 2041, 2319, 2405, 2462, 3095
PfoI TCCNGGA 1 cut(s) 2850
PkrI GCNGC 3 cut(s) 631, 672, 2369
PleI GAGTC 5 cut(s) 226, 837, 1394, 1876, 2899
PmeI GTTTAAAC 1 cut(s) 2533
PpsI GAGTC 5 cut(s) 226, 837, 1394, 1876, 2899
Ppu21I YACGTR 1 cut(s) 2471
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 2 cut(s) 2055, 2545
PshBI ATTAAT 4 cut(s) 1194, 1227, 1956, 2421
PsiI TTATAA 3 cut(s) 1617, 2343, 2354
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 1804, 2850
PspFI CCCAGC 1 cut(s) 38
PspGI CCWGG 2 cut(s) 1804, 2850
PspN4I GGNNCC 4 cut(s) 317, 448, 2430, 3051
PspPI GGNCC 2 cut(s) 315, 2518
PspPPI RGGWCCY 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 2951
PsuI RGATCY 4 cut(s) 1144, 1269, 2290, 3049
RsaI GTAC 8 cut(s) 448, 1309, 1469, 1519, 1535, 2214, 2430, 2740
RsaNI GTAC 8 cut(s) 447, 1308, 1468, 1518, 1534, 2213, 2429, 2739
RseI CAYNNNNRTG 3 cut(s) 288, 1013, 1670
SatI GCNGC 3 cut(s) 630, 671, 2368
Sau3AI GATC 7 cut(s) 1144, 1269, 1836, 2100, 2290, 3004, 3049
Sau96I GGNCC 2 cut(s) 315, 2518
SchI GAGTC 5 cut(s) 227, 838, 1395, 1877, 2900
ScrFI CCNGG 2 cut(s) 1806, 2852
SduI GDGCHC 3 cut(s) 271, 709, 2707
SfaNI GCATC 7 cut(s) 286, 1444, 2068, 2215, 2377, 2482, 2587
SfcI CTRYAG 3 cut(s) 793, 2115, 3026
SfuI TTCGAA 1 cut(s) 923
SinI GGWCC 2 cut(s) 315, 2518
SmiMI CAYNNNNRTG 3 cut(s) 288, 1013, 1670
SmlI CTYRAG 4 cut(s) 433, 1565, 2522, 2821
SmoI CTYRAG 4 cut(s) 433, 1565, 2522, 2821
SnaBI TACGTA 1 cut(s) 2471
SpeI ACTAGT 1 cut(s) 2017
SseBI AGGCCT 1 cut(s) 2641
SsiI CCGC 2 cut(s) 1256, 2368
SspI AATATT 1 cut(s) 2332
SspMI CTAG 5 cut(s) 983, 1169, 2018, 2288, 3243
StuI AGGCCT 1 cut(s) 2641
StyD4I CCNGG 2 cut(s) 1804, 2850
StyI CCWWGG 2 cut(s) 2371, 2790
TaiI ACGT 2 cut(s) 121, 2473
TaqI TCGA 4 cut(s) 221, 780, 923, 3221
TatI WGTACW 3 cut(s) 1307, 1467, 1517
TauI GCSGC 1 cut(s) 2370
TfiI GAWTC 8 cut(s) 183, 349, 1023, 2041, 2319, 2405, 2462, 3095
TscAI CASTG 8 cut(s) 594, 1896, 2284, 2659, 2725, 2922, 2952, 3219
TseFI GTSAC 2 cut(s) 2947, 3038
TseI GCWGC 2 cut(s) 629, 670
Tsp45I GTSAC 2 cut(s) 2947, 3038
TspRI CASTG 8 cut(s) 594, 1896, 2284, 2659, 2725, 2922, 2952, 3219
VpaK11BI GGWCC 2 cut(s) 315, 2518
VspI ATTAAT 4 cut(s) 1194, 1227, 1956, 2421
XagI CCTNNNNNAGG 1 cut(s) 2261
XapI RAATTY 9 cut(s) 337, 1100, 1241, 1501, 1774, 1795, 2648, 2710, 2767
XceI RCATGY 4 cut(s) 358, 1614, 2059, 2549
XcmI CCANNNNNNNNNTGG 3 cut(s) 1015, 1369, 2797
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 2832
XspI CTAG 5 cut(s) 983, 1169, 2018, 2288, 3243
Zsp2I ATGCAT 2 cut(s) 2230, 2392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.