Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008661

Physical Location & Seq

Genomic Coordinates Forward (+)

28044242 .. 28045079

Gene Structure 838 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1250300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 762 bp

ATGATAATCTTCTCTCCAATCTTCCACACCAGACTAATTCGATCCTTTTCTTCTTCTTCTTGCCGAACCAGTCTTCCGGAACCCCTACCCCTTCAAACTCTACTCAAAGGCGGCTTCACTCCCACGCTCAAATCCATCGTCCAATTCCTCTCTCGAACCCGCCGGTTCAACACCCTCCTCCACTTCTTCTCCCAAATGGAGTCCAACCAAATCAAAGGCAACTCCCAGGCGCACTCCATCGTGACCTATTCTGCAGAAATACGAAGAAGCAGAGCATTTTATGAGGACCCAGATGACCGAAGCTTCGAAGTTGCGGCGGAATCGGATGTGGGACTCTCTCATTCAAGGCCTGTGTATCAACCGGAAAGACCCCGAGAAGGCTTTGTTGGTGTTGCGGGATTCTTGGGGACCCACGGTATTTTTCCTTCTTCTTTCACGTTTTGCTCGTTAATTCATTCGTTTAGCAATGAGGGAGATATGAGCAAGGCAATTGAGGTGCTGGACTTGATGACTGATGAGAAAGTTAAGTACCCTTTTGATAATTTTGTTTCTAGTTCAGTAATTTCCGGGTTTTGTAAGATTGGGAAGCCGGAAATCGCAGTCAAATTTCTTGAGAATGCCGTGGATTCAGCAGCTTTGGAGCCTAATATTGTGACTTATGCAGCACTAGTTGCTGCTCTTTGTAAATTGGGCAGAGTTAATGAGGTTTGTGATTTGGTTTGTAGGATTGAGAAGCAAGGTTTGGCATTTTGTTTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

254

Amino Acids

28.13

Weight (kDa)

7.0

Isoelectric Point (pI)

48.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	139 - 170	1.4e-06	PPR repeat
PPR_2	PF13041	186 - 229	3.1e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57250
fragaria_vesca	FvH4_4g07420
malus_domestica	MD13G1243700.v1.1 MD16G1250300.v1.1 MD16G1250400.v1.1 MD16G1250500.v1.1 MD16G1250600.v1.1
prunus_persica	Prupe.1G077100_v2.0.a1
pyrus_communis	pycom13g21510 pycom16g21000
rosa_chinensis	RchiOBHm_Chr2g0131261 RchiOBHm_Chr4g0399881
rosa_laevigata	RLG00000009236
rosa_multiflora	Rmu_sc0014676.1_g000003
rosa_roxburghii	Rroxscaffold_5G00344750
rosa_rugosa	Rorug04G0019700
rosa_samantha	Rh2BG356200 Rh4AG096800 Rh4BG094300 Rh4CG105900 Rh4DG089200 Rh4DG091000
rosa_wichuraiana	Rw4G008000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	76
AciI	CCGC	5 cut(s)	111, 160, 314, 317, 395
AclWI	GGATC	1 cut(s)	36
AcsI	RAATTY	1 cut(s)	605
AfaI	GTAC	1 cut(s)	530
AfiI	CCNNNNNNNGG	1 cut(s)	377
AgsI	TTSAA	3 cut(s)	95, 169, 345
AhlI	ACTAGT	1 cut(s)	667
AjnI	CCWGG	1 cut(s)	225
AjuI	GAANNNNNNNTTGG	4 cut(s)	369, 401, 725, 757
AloI	GAACNNNNNNTCC	2 cut(s)	58, 90
AluBI	AGCT	2 cut(s)	303, 635
AluI	AGCT	2 cut(s)	303, 635
AlwI	GGATC	1 cut(s)	36
Ama87I	CYCGRG	1 cut(s)	372
Aor13HI	TCCGGA	1 cut(s)	76
AoxI	GGCC	1 cut(s)	347
ApeKI	GCWGC	3 cut(s)	632, 662, 674
ApoI	RAATTY	1 cut(s)	605
ArsI	GACNNNNNNTTYG	4 cut(s)	123, 155, 287, 319
AspLEI	GCGC	1 cut(s)	232
AspS9I	GGNCC	2 cut(s)	286, 408
AsuC2I	CCSGG	1 cut(s)	568
AsuII	TTCGAA	1 cut(s)	306
AvaI	CYCGRG	1 cut(s)	372
AvaII	GGWCC	2 cut(s)	286, 408
BarI	GAAGNNNNNNTAC	2 cut(s)	409, 441
BbsI	GAAGAC	1 cut(s)	65
BbvI	GCAGC	3 cut(s)	644, 661, 674
BccI	CCATC	2 cut(s)	143, 245
BceAI	ACGGC	1 cut(s)	605
BciT130I	CCWGG	1 cut(s)	227
BcnI	CCSGG	1 cut(s)	568
BcuI	ACTAGT	1 cut(s)	667
BfaI	CTAG	2 cut(s)	552, 668
BfmI	CTRYAG	1 cut(s)	252
BisI	GCNGC	5 cut(s)	112, 315, 633, 663, 675
BlsI	GCNGC	5 cut(s)	113, 316, 634, 664, 676
Bme1390I	CCNGG	2 cut(s)	227, 568
Bme18I	GGWCC	2 cut(s)	286, 408
BmeT110I	CYCGRG	1 cut(s)	372
BmgT120I	GGNCC	2 cut(s)	286, 408
BmiI	GGNNCC	5 cut(s)	81, 288, 409, 410, 642
BmrFI	CCNGG	2 cut(s)	227, 568
BpiI	GAAGAC	1 cut(s)	65
Bpu14I	TTCGAA	1 cut(s)	306
BpuEI	CTTGAG	1 cut(s)	632
BpuMI	CCSGG	1 cut(s)	568
BsaJI	CCNNGG	3 cut(s)	225, 412, 621
BsaWI	WCCGGW	2 cut(s)	76, 361
BsaXI	ACNNNNNCTCC	2 cut(s)	173, 203
Bsc4I	CCNNNNNNNGG	1 cut(s)	377
Bse118I	RCCGGY	1 cut(s)	162
Bse1I	ACTGG	1 cut(s)	69
Bse3DI	GCAATG	1 cut(s)	472
BseAI	TCCGGA	1 cut(s)	76
BseBI	CCWGG	1 cut(s)	227
BseDI	CCNNGG	3 cut(s)	225, 412, 621
BseGI	GGATG	1 cut(s)	331
BseLI	CCNNNNNNNGG	1 cut(s)	377
BseMI	GCAATG	1 cut(s)	472
BseNI	ACTGG	1 cut(s)	69
BseRI	GAGGAG	1 cut(s)	167
BseXI	GCAGC	3 cut(s)	644, 661, 674
BshFI	GGCC	1 cut(s)	349
BsiHKCI	CYCGRG	1 cut(s)	372
BsiSI	CCGG	5 cut(s)	77, 163, 362, 567, 590
BslFI	GGGAC	2 cut(s)	345, 421
BslI	CCNNNNNNNGG	1 cut(s)	377
BsmFI	GGGAC	2 cut(s)	345, 421
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	1 cut(s)	349
BsoBI	CYCGRG	1 cut(s)	372
Bsp119I	TTCGAA	1 cut(s)	306
Bsp13I	TCCGGA	1 cut(s)	76
Bsp143I	GATC	1 cut(s)	41
BspACI	CCGC	5 cut(s)	111, 160, 314, 317, 395
BspANI	GGCC	1 cut(s)	349
BspEI	TCCGGA	1 cut(s)	76
BspLI	GGNNCC	5 cut(s)	81, 288, 409, 410, 642
BspMAI	CTGCAG	1 cut(s)	256
BspPI	GGATC	1 cut(s)	36
BspT104I	TTCGAA	1 cut(s)	306
BsrDI	GCAATG	1 cut(s)	472
BsrFI	RCCGGY	1 cut(s)	162
BsrI	ACTGG	1 cut(s)	69
BssAI	RCCGGY	1 cut(s)	162
BssECI	CCNNGG	3 cut(s)	225, 412, 621
BssMI	GATC	1 cut(s)	41
Bst2UI	CCWGG	1 cut(s)	227
Bst4CI	ACNGT	1 cut(s)	416
BstAPI	GCANNNNNTGC	1 cut(s)	671
BstBI	TTCGAA	1 cut(s)	306
BstDSI	CCRYGG	2 cut(s)	412, 621
BstF5I	GGATG	1 cut(s)	331
BstHHI	GCGC	1 cut(s)	232
BstKTI	GATC	1 cut(s)	44
BstMBI	GATC	1 cut(s)	41
BstMWI	GCNNNNNNNGC	1 cut(s)	671
BstNI	CCWGG	1 cut(s)	227
BstSCI	CCNGG	2 cut(s)	225, 566
BstSFI	CTRYAG	1 cut(s)	252
BstV1I	GCAGC	3 cut(s)	644, 661, 674
BstV2I	GAAGAC	1 cut(s)	65
BsuRI	GGCC	1 cut(s)	349
BtgI	CCRYGG	2 cut(s)	412, 621
BtsCI	GGATG	1 cut(s)	331
CfoI	GCGC	1 cut(s)	232
Cfr10I	RCCGGY	1 cut(s)	162
Cfr13I	GGNCC	2 cut(s)	286, 408
Csp6I	GTAC	1 cut(s)	529
CviJI	RGCY	7 cut(s)	114, 303, 349, 381, 589, 635, 643
CviKI_1	RGCY	7 cut(s)	114, 303, 349, 381, 589, 635, 643
CviQI	GTAC	1 cut(s)	529
DpnI	GATC	1 cut(s)	43
DpnII	GATC	1 cut(s)	41
EciI	GGCGGA	1 cut(s)	332
Eco147I	AGGCCT	1 cut(s)	349
Eco47I	GGWCC	2 cut(s)	286, 408
Eco88I	CYCGRG	1 cut(s)	372
EcoO109I	RGGNCCY	2 cut(s)	286, 408
EcoRII	CCWGG	1 cut(s)	225
FaiI	YATR	3 cut(s)	282, 479, 660
FaqI	GGGAC	2 cut(s)	345, 421
FauI	CCCGC	2 cut(s)	167, 388
Fnu4HI	GCNGC	5 cut(s)	112, 315, 633, 663, 675
FokI	GGATG	1 cut(s)	338
Fsp4HI	GCNGC	5 cut(s)	112, 315, 633, 663, 675
FspBI	CTAG	2 cut(s)	552, 668
GlaI	GCGC	1 cut(s)	231
GluI	GCNGC	5 cut(s)	112, 315, 633, 663, 675
HaeIII	GGCC	1 cut(s)	349
HapII	CCGG	5 cut(s)	77, 163, 362, 567, 590
HhaI	GCGC	1 cut(s)	232
Hin6I	GCGC	1 cut(s)	230
HinP1I	GCGC	1 cut(s)	230
HindIII	AAGCTT	1 cut(s)	301
HinfI	GANTC	5 cut(s)	200, 320, 333, 399, 626
HpaII	CCGG	5 cut(s)	77, 163, 362, 567, 590
Hpy188I	TCNGA	1 cut(s)	325
Hpy188III	TCNNGA	4 cut(s)	77, 153, 241, 611
HpyAV	CCTTC	3 cut(s)	101, 371, 435
HpyCH4III	ACNGT	1 cut(s)	416
HpyCH4IV	ACGT	1 cut(s)	437
HpyCH4V	TGCA	2 cut(s)	254, 662
HpyF10VI	GCNNNNNNNGC	1 cut(s)	671
HpySE526I	ACGT	1 cut(s)	437
HspAI	GCGC	1 cut(s)	230
KflI	GGGWCCC	1 cut(s)	408
Kpn2I	TCCGGA	1 cut(s)	76
Kzo9I	GATC	1 cut(s)	41
LmnI	GCTCC	1 cut(s)	640
Lsp1109I	GCAGC	3 cut(s)	644, 661, 674
MaeI	CTAG	2 cut(s)	552, 668
MaeII	ACGT	1 cut(s)	437
MaeIII	GTNAC	2 cut(s)	241, 652
MalI	GATC	1 cut(s)	43
MboI	GATC	1 cut(s)	41
MboII	GAAGA	8 cut(s)	13, 42, 45, 48, 65, 178, 276, 420
MfeI	CAATTG	1 cut(s)	489
MluCI	AATT	8 cut(s)	36, 143, 450, 489, 541, 561, 605, 686
MlyI	GAGTC	2 cut(s)	209, 327
MmeI	TCCRAC	1 cut(s)	228
MnlI	CCTC	7 cut(s)	158, 185, 188, 277, 463, 487, 697
MroI	TCCGGA	1 cut(s)	76
MseI	TTAA	3 cut(s)	449, 525, 699
MspI	CCGG	5 cut(s)	77, 163, 362, 567, 590
MspR9I	CCNGG	2 cut(s)	227, 568
MunI	CAATTG	1 cut(s)	489
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	1 cut(s)	227
MwoI	GCNNNNNNNGC	1 cut(s)	671
NciI	CCSGG	1 cut(s)	568
NdeII	GATC	1 cut(s)	41
NlaIV	GGNNCC	5 cut(s)	81, 288, 409, 410, 642
NmuCI	GTSAC	2 cut(s)	241, 652
NspV	TTCGAA	1 cut(s)	306
PceI	AGGCCT	1 cut(s)	349
PcsI	WCGNNNNNNNCGW	1 cut(s)	443
PctI	GAATGC	1 cut(s)	622
PfeI	GAWTC	3 cut(s)	320, 399, 626
PkrI	GCNGC	5 cut(s)	113, 316, 634, 664, 676
PleI	GAGTC	2 cut(s)	208, 327
PpsI	GAGTC	2 cut(s)	208, 327
PpuMI	RGGWCCY	2 cut(s)	286, 408
Psp5II	RGGWCCY	2 cut(s)	286, 408
Psp6I	CCWGG	1 cut(s)	225
PspGI	CCWGG	1 cut(s)	225
PspN4I	GGNNCC	5 cut(s)	81, 288, 409, 410, 642
PspPI	GGNCC	2 cut(s)	286, 408
PspPPI	RGGWCCY	2 cut(s)	286, 408
PstI	CTGCAG	1 cut(s)	256
RsaI	GTAC	1 cut(s)	530
RsaNI	GTAC	1 cut(s)	529
SaqAI	TTAA	3 cut(s)	449, 525, 699
SatI	GCNGC	5 cut(s)	112, 315, 633, 663, 675
Sau3AI	GATC	1 cut(s)	41
Sau96I	GGNCC	2 cut(s)	286, 408
SchI	GAGTC	2 cut(s)	209, 327
ScrFI	CCNGG	2 cut(s)	227, 568
SetI	ASST	7 cut(s)	248, 305, 440, 498, 637, 708, 742
SfcI	CTRYAG	1 cut(s)	252
SfuI	TTCGAA	1 cut(s)	306
SinI	GGWCC	2 cut(s)	286, 408
SmlI	CTYRAG	1 cut(s)	611
SmoI	CTYRAG	1 cut(s)	611
SpeI	ACTAGT	1 cut(s)	667
Sse9I	AATT	8 cut(s)	36, 143, 450, 489, 541, 561, 605, 686
SseBI	AGGCCT	1 cut(s)	349
SsiI	CCGC	5 cut(s)	111, 160, 314, 317, 395
SspI	AATATT	1 cut(s)	649
SspMI	CTAG	2 cut(s)	552, 668
StuI	AGGCCT	1 cut(s)	349
StyD4I	CCNGG	2 cut(s)	225, 566
TaaI	ACNGT	1 cut(s)	416
TaiI	ACGT	1 cut(s)	440
TaqI	TCGA	3 cut(s)	40, 154, 306
TaqII	GACCGA	1 cut(s)	312
TasI	AATT	8 cut(s)	36, 143, 450, 489, 541, 561, 605, 686
TauI	GCSGC	2 cut(s)	114, 317
TfiI	GAWTC	3 cut(s)	320, 399, 626
Tru1I	TTAA	3 cut(s)	449, 525, 699
Tru9I	TTAA	3 cut(s)	449, 525, 699
TseFI	GTSAC	2 cut(s)	241, 652
TseI	GCWGC	3 cut(s)	632, 662, 674
Tsp45I	GTSAC	2 cut(s)	241, 652
TspDTI	ATGAA	1 cut(s)	443
VpaK11BI	GGWCC	2 cut(s)	286, 408
XapI	RAATTY	1 cut(s)	605
XspI	CTAG	2 cut(s)	552, 668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.