Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003603

Physical Location & Seq

Genomic Coordinates Forward (+)

25757188 .. 25761457

Gene Structure 4270 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1245600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGGAAGATTACACTCTCGTTGCGAGGAAGTCTTGTTTCGGCTTGCCCACTGCTTGCCCTTTATGCTTGCCCGTCTACTTCTACCTCAAACTTACCAACGTTCCCTTCCGATTAGATTTCAATCTCACCTACCCTGACTCTGATAAAATCCCTTATATTGAATCTGGCGAGTATGTGGCCTATAATAACGAGAAGGGTGGCATTATCGAAAGACTAAAAGAAGACCGTATTGCTGATTTGGAAACTGAATTTCACTCAATACCAGAATGGATATCTACAAAGGCAATGATTAGCTCCTGGGTTTCTGATGCTCTTACGTATGAACTCTGGGTTGGAACACATGGTAATTCTGCCAAAGAGATATATTATTCTGATCTCCCATGGCCAATTGGAAAGATTCTATATTATAAGAAATCTTACACCGTAAAGCAGCAGCTTGGGATTACTAATGACAACGCTGAGAAAATAGAAGAACAGACATACAGGAGAGCCAGCCTTGCATTTGGAGCTCTATCAAGTCGGTTGGAGGAACATAAGTTTTTATTTGAAGACAGGCCGTCAAGTTTGGATGCTCTCTTTCTTGGGCAGGCACTTGTCATTCTTCAAGCATTACCAGAAACATCGGTACTGCGGAGCAAGCTTTTAGAACATGAAAACCTTGTTAAGTATGCTGATAGGCTTAAGGTGGAGTTCATGGACGCAGGTTCTTCATCTTCAGTCCCAAATTTTCATGCAAACCCTTCATCAGCAGCTCCTAGAAGAGGTCCTTCAAATTCGAGTTCTAAGCCCAAGAGCAAACCCAAAAGGGAAAAGACAAAGGAGGAGAAAAGTTTTAAGAGCAGGGCCAGATATTTTATAATAGCACAGCTAGTTTCAGTTGTACTTTTCGTCACTCTTCTAAGCCGAGTTGATGAGGCTGATGCCGAGGTTGATGATGATGATGGCTATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0009536 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0019866 GO:0019867 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GST_C_6 GST_N_4

Protein Analysis

318

Amino Acids

36.02

Weight (kDa)

5.59

Isoelectric Point (pI)

41.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_4	PF17172	20 - 112	5.5e-16	Glutathione S-transferase N-terminal domain
GST_C_6	PF17171	166 - 227	3e-16	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19080 AT2G19080
fragaria_vesca	FvH4_4g07590 FvH4_4g07590 FvH4_4g07590 FvH4_4g07590 FvH4_4g07590 FvH4_4g07590 FvH4_4g07590
malus_domestica	MD13G1245600.v1.1
prunus_persica	Prupe.1G079200_v2.0.a1
pyrus_communis	pycom13g21680 pycom16g21400
rosa_chinensis	RchiOBHm_Chr4g0400191 RchiOBHm_Chr7g0191431 RchiOBHm_Chr7g0191541
rosa_laevigata	RLG00000004440 RLG00000009205
rosa_multiflora	Rmu_co8142490.1_g000001 Rmu_sc0000037.1_g000022 Rmu_sc0002661.1_g000008 Rmu_sc0003634.1_g000010 Rmu_sc0010599.1_g000007
rosa_roxburghii	Rroxscaffold_5G00345140
rosa_rugosa	Rorug04G0023500 Rorug04G0023600 Rorug04G0023700
rosa_samantha	Rh4AG098800 Rh4BG097100 Rh4CG108300 Rh4DG093800 Rh7AG119100 Rh7CG124100 Rh7DG122200
rosa_wichuraiana	Rw4G008210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	408, 857
Acc36I	ACCTGC	1 cut(s)	692
AccI	GTMKAC	1 cut(s)	75
AciI	CCGC	1 cut(s)	631
AclI	AACGTT	1 cut(s)	99
AcoI	YGGCCR	1 cut(s)	383
AcsI	RAATTY	3 cut(s)	248, 724, 772
AcuI	CTGAAG	1 cut(s)	699
AfaI	GTAC	2 cut(s)	627, 882
AfiI	CCNNNNNNNGG	1 cut(s)	761
AflII	CTTAAG	1 cut(s)	680
AgsI	TTSAA	5 cut(s)	121, 161, 548, 605, 771
AhdI	GACNNNNNGTC	1 cut(s)	556
AjnI	CCWGG	1 cut(s)	296
AjuI	GAANNNNNNNTTGG	4 cut(s)	89, 121, 315, 347
AluBI	AGCT	6 cut(s)	294, 436, 509, 640, 752, 868
AluI	AGCT	6 cut(s)	294, 436, 509, 640, 752, 868
Alw21I	GWGCWC	1 cut(s)	511
AoxI	GGCC	4 cut(s)	177, 383, 554, 843
ApeKI	GCWGC	3 cut(s)	430, 433, 749
ApoI	RAATTY	3 cut(s)	248, 724, 772
AspS9I	GGNCC	2 cut(s)	764, 843
AsuHPI	GGTGA	1 cut(s)	118
AvaII	GGWCC	1 cut(s)	764
BalI	TGGCCA	1 cut(s)	385
BanII	GRGCYC	1 cut(s)	511
BbsI	GAAGAC	2 cut(s)	228, 555
Bbv12I	GWGCWC	1 cut(s)	511
BbvI	GCAGC	3 cut(s)	442, 445, 761
BccI	CCATC	1 cut(s)	935
BceAI	ACGGC	1 cut(s)	541
BciT130I	CCWGG	1 cut(s)	298
BfaI	CTAG	2 cut(s)	756, 869
BfrI	CTTAAG	1 cut(s)	680
BfuAI	ACCTGC	1 cut(s)	692
BisI	GCNGC	3 cut(s)	431, 434, 750
BlsI	GCNGC	3 cut(s)	432, 435, 751
Bme1390I	CCNGG	1 cut(s)	298
Bme18I	GGWCC	1 cut(s)	764
BmeRI	GACNNNNNGTC	1 cut(s)	556
BmgT120I	GGNCC	2 cut(s)	764, 843
BmrFI	CCNGG	1 cut(s)	298
BmsI	GCATC	3 cut(s)	298, 559, 910
BpiI	GAAGAC	2 cut(s)	228, 555
BsaAI	YACGTR	1 cut(s)	318
BsaBI	GATNNNNATC	1 cut(s)	120
BsaJI	CCNNGG	3 cut(s)	297, 380, 924
Bsc4I	CCNNNNNNNGG	1 cut(s)	761
Bse3DI	GCAATG	1 cut(s)	291
Bse8I	GATNNNNATC	1 cut(s)	120
BseBI	CCWGG	1 cut(s)	298
BseDI	CCNNGG	3 cut(s)	297, 380, 924
BseGI	GGATG	1 cut(s)	574
BseJI	GATNNNNATC	1 cut(s)	120
BseLI	CCNNNNNNNGG	1 cut(s)	761
BseMI	GCAATG	1 cut(s)	291
BseMII	CTCAG	1 cut(s)	450
BseRI	GAGGAG	1 cut(s)	836
BseXI	GCAGC	3 cut(s)	442, 445, 761
BshFI	GGCC	4 cut(s)	179, 385, 556, 845
BsiHKAI	GWGCWC	1 cut(s)	511
BslFI	GGGAC	1 cut(s)	704
BslI	CCNNNNNNNGG	1 cut(s)	761
BsmFI	GGGAC	1 cut(s)	704
BsnI	GGCC	4 cut(s)	179, 385, 556, 845
Bsp1286I	GDGCHC	1 cut(s)	511
Bsp143I	GATC	1 cut(s)	373
Bsp19I	CCATGG	1 cut(s)	380
BspACI	CCGC	1 cut(s)	631
BspANI	GGCC	4 cut(s)	179, 385, 556, 845
BspCNI	CTCAG	1 cut(s)	451
BspMI	ACCTGC	1 cut(s)	692
BspTI	CTTAAG	1 cut(s)	680
BsrDI	GCAATG	1 cut(s)	291
BssECI	CCNNGG	3 cut(s)	297, 380, 924
BssMI	GATC	1 cut(s)	373
BssT1I	CCWWGG	1 cut(s)	380
Bst2UI	CCWGG	1 cut(s)	298
Bst4CI	ACNGT	2 cut(s)	227, 424
Bst6I	CTCTTC	2 cut(s)	754, 900
BstAFI	CTTAAG	1 cut(s)	680
BstBAI	YACGTR	1 cut(s)	318
BstC8I	GCNNGC	6 cut(s)	44, 55, 68, 493, 588, 638
BstDEI	CTNAG	3 cut(s)	459, 783, 899
BstDSI	CCRYGG	1 cut(s)	380
BstENI	CCTNNNNNAGG	1 cut(s)	759
BstF5I	GGATG	1 cut(s)	574
BstKTI	GATC	1 cut(s)	376
BstMBI	GATC	1 cut(s)	373
BstMWI	GCNNNNNNNGC	4 cut(s)	63, 497, 506, 637
BstNI	CCWGG	1 cut(s)	298
BstSCI	CCNGG	1 cut(s)	296
BstSNI	TACGTA	1 cut(s)	318
BstV1I	GCAGC	3 cut(s)	442, 445, 761
BstV2I	GAAGAC	2 cut(s)	228, 555
BsuRI	GGCC	4 cut(s)	179, 385, 556, 845
BtgI	CCRYGG	1 cut(s)	380
BtsCI	GGATG	1 cut(s)	574
BtsI	GCAGTG	1 cut(s)	48
BtsIMutI	CAGTG	1 cut(s)	48
BveI	ACCTGC	1 cut(s)	692
Cac8I	GCNNGC	6 cut(s)	44, 55, 68, 493, 588, 638
Cfr13I	GGNCC	2 cut(s)	764, 843
CseI	GACGC	1 cut(s)	707
Csp6I	GTAC	2 cut(s)	626, 881
CviAII	CATG	5 cut(s)	341, 381, 650, 694, 731
CviQI	GTAC	2 cut(s)	626, 881
DdeI	CTNAG	3 cut(s)	459, 783, 899
DpnI	GATC	1 cut(s)	375
DpnII	GATC	1 cut(s)	373
DriI	GACNNNNNGTC	1 cut(s)	556
EaeI	YGGCCR	1 cut(s)	383
Eam1104I	CTCTTC	2 cut(s)	754, 900
Eam1105I	GACNNNNNGTC	1 cut(s)	556
EarI	CTCTTC	2 cut(s)	754, 900
Ecl136II	GAGCTC	1 cut(s)	509
Eco105I	TACGTA	1 cut(s)	318
Eco130I	CCWWGG	1 cut(s)	380
Eco24I	GRGCYC	1 cut(s)	511
Eco32I	GATATC	1 cut(s)	273
Eco47I	GGWCC	1 cut(s)	764
Eco53kI	GAGCTC	1 cut(s)	509
Eco57I	CTGAAG	1 cut(s)	699
EcoICRI	GAGCTC	1 cut(s)	509
EcoNI	CCTNNNNNAGG	1 cut(s)	759
EcoO109I	RGGNCCY	1 cut(s)	764
EcoRII	CCWGG	1 cut(s)	296
EcoRV	GATATC	1 cut(s)	273
EcoT14I	CCWWGG	1 cut(s)	380
EcoT38I	GRGCYC	1 cut(s)	511
ErhI	CCWWGG	1 cut(s)	380
FaeI	CATG	5 cut(s)	344, 384, 653, 697, 734
FalI	AAGNNNNNCTT	2 cut(s)	751, 783
FaqI	GGGAC	1 cut(s)	704
FatI	CATG	5 cut(s)	340, 380, 649, 693, 730
FblI	GTMKAC	1 cut(s)	75
Fnu4HI	GCNGC	3 cut(s)	431, 434, 750
FokI	GGATG	1 cut(s)	581
FriOI	GRGCYC	1 cut(s)	511
Fsp4HI	GCNGC	3 cut(s)	431, 434, 750
FspBI	CTAG	2 cut(s)	756, 869
GluI	GCNGC	3 cut(s)	431, 434, 750
HaeIII	GGCC	4 cut(s)	179, 385, 556, 845
HgaI	GACGC	1 cut(s)	707
Hin1II	CATG	5 cut(s)	344, 384, 653, 697, 734
HindIII	AAGCTT	1 cut(s)	638
HinfI	GANTC	3 cut(s)	137, 161, 397
HphI	GGTGA	1 cut(s)	118
Hpy166II	GTNNAC	1 cut(s)	76
Hpy188I	TCNGA	4 cut(s)	110, 142, 307, 373
Hpy8I	GTNNAC	1 cut(s)	76
HpyAV	CCTTC	4 cut(s)	115, 187, 750, 777
HpyCH4III	ACNGT	2 cut(s)	227, 424
HpyCH4IV	ACGT	2 cut(s)	99, 317
HpyCH4V	TGCA	2 cut(s)	500, 734
HpyF10VI	GCNNNNNNNGC	4 cut(s)	63, 497, 506, 637
HpyF3I	CTNAG	3 cut(s)	459, 783, 899
HpySE526I	ACGT	2 cut(s)	99, 317
Hsp92II	CATG	5 cut(s)	344, 384, 653, 697, 734
Kzo9I	GATC	1 cut(s)	373
LmnI	GCTCC	4 cut(s)	299, 506, 633, 757
Lsp1109I	GCAGC	3 cut(s)	442, 445, 761
LweI	GCATC	3 cut(s)	298, 559, 910
MaeI	CTAG	2 cut(s)	756, 869
MaeII	ACGT	2 cut(s)	99, 317
MaeIII	GTNAC	1 cut(s)	889
MalI	GATC	1 cut(s)	375
MboI	GATC	1 cut(s)	373
MboII	GAAGA	9 cut(s)	17, 233, 482, 560, 593, 699, 705, 771, 887
MfeI	CAATTG	1 cut(s)	387
MhlI	GDGCHC	1 cut(s)	511
MlsI	TGGCCA	1 cut(s)	385
MluCI	AATT	5 cut(s)	248, 346, 387, 724, 772
MluNI	TGGCCA	1 cut(s)	385
MlyI	GAGTC	1 cut(s)	131
MmeI	TCCRAC	2 cut(s)	313, 504
MnlI	CCTC	7 cut(s)	18, 95, 520, 755, 814, 907, 919
Mox20I	TGGCCA	1 cut(s)	385
MscI	TGGCCA	1 cut(s)	385
MseI	TTAA	3 cut(s)	663, 681, 834
Msp20I	TGGCCA	1 cut(s)	385
MspCI	CTTAAG	1 cut(s)	680
MspR9I	CCNGG	1 cut(s)	298
MunI	CAATTG	1 cut(s)	387
MvaI	CCWGG	1 cut(s)	298
MwoI	GCNNNNNNNGC	4 cut(s)	63, 497, 506, 637
NcoI	CCATGG	1 cut(s)	380
NdeII	GATC	1 cut(s)	373
NlaIII	CATG	5 cut(s)	344, 384, 653, 697, 734
NmeAIII	GCCGAG	2 cut(s)	929, 949
NmuCI	GTSAC	1 cut(s)	889
PfeI	GAWTC	2 cut(s)	161, 397
PkrI	GCNGC	3 cut(s)	432, 435, 751
PleI	GAGTC	1 cut(s)	131
PpsI	GAGTC	1 cut(s)	131
Ppu21I	YACGTR	1 cut(s)	318
PpuMI	RGGWCCY	1 cut(s)	764
PsiI	TTATAA	2 cut(s)	408, 857
Psp124BI	GAGCTC	1 cut(s)	511
Psp1406I	AACGTT	1 cut(s)	99
Psp5II	RGGWCCY	1 cut(s)	764
Psp6I	CCWGG	1 cut(s)	296
PspGI	CCWGG	1 cut(s)	296
PspPI	GGNCC	2 cut(s)	764, 843
PspPPI	RGGWCCY	1 cut(s)	764
RsaI	GTAC	2 cut(s)	627, 882
RsaNI	GTAC	2 cut(s)	626, 881
SacI	GAGCTC	1 cut(s)	511
SaqAI	TTAA	3 cut(s)	663, 681, 834
SatI	GCNGC	3 cut(s)	431, 434, 750
Sau3AI	GATC	1 cut(s)	373
Sau96I	GGNCC	2 cut(s)	764, 843
SchI	GAGTC	1 cut(s)	131
ScrFI	CCNGG	1 cut(s)	298
SduI	GDGCHC	1 cut(s)	511
SfaNI	GCATC	3 cut(s)	298, 559, 910
SinI	GGWCC	1 cut(s)	764
SmlI	CTYRAG	1 cut(s)	680
SmoI	CTYRAG	1 cut(s)	680
SnaBI	TACGTA	1 cut(s)	318
Sse9I	AATT	5 cut(s)	248, 346, 387, 724, 772
SsiI	CCGC	1 cut(s)	631
SspMI	CTAG	2 cut(s)	756, 869
SstI	GAGCTC	1 cut(s)	511
StyD4I	CCNGG	1 cut(s)	296
StyI	CCWWGG	1 cut(s)	380
TaaI	ACNGT	2 cut(s)	227, 424
TaiI	ACGT	2 cut(s)	102, 320
TaqI	TCGA	2 cut(s)	207, 776
TasI	AATT	5 cut(s)	248, 346, 387, 724, 772
TatI	WGTACW	1 cut(s)	880
TfiI	GAWTC	2 cut(s)	161, 397
Tru1I	TTAA	3 cut(s)	663, 681, 834
Tru9I	TTAA	3 cut(s)	663, 681, 834
TscAI	CASTG	1 cut(s)	55
TseFI	GTSAC	1 cut(s)	889
TseI	GCWGC	3 cut(s)	430, 433, 749
Tsp45I	GTSAC	1 cut(s)	889
TspDTI	ATGAA	6 cut(s)	336, 666, 682, 699, 719, 732
TspRI	CASTG	1 cut(s)	55
Vha464I	CTTAAG	1 cut(s)	680
VpaK11BI	GGWCC	1 cut(s)	764
XagI	CCTNNNNNAGG	1 cut(s)	759
XapI	RAATTY	3 cut(s)	248, 724, 772
XmiI	GTMKAC	1 cut(s)	75
XspI	CTAG	2 cut(s)	756, 869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.