Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009440

Physical Location & Seq

Genomic Coordinates Reverse (-)

27337031 .. 27340649

Gene Structure 3619 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1253800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 726 bp

ATGGCGAATCCCTCCCAAGCTGTCGGCGGCAGAGGACCAACCACTCATGTCATCTTCGATATGGACGGCCTCTTACTCGACACAGAGAAGTTTTATACTGAAGTCCAGGAAAAAATACTTGCTAGGTATAATAAAACTTTTGATTGGTCTCTCAAAGCGAAAATGATGGGAAAGAAAGCAATCGAAGCTGCTCGGGTGTTTGTTGAAGAATCTGGAATTAGTGACACCCTAACTGCTGAGGGTTTTCTAGTTGAGAGAGAGGCGGTGTTGCAGAGTTTGTTTCCAACAAGTGAGCTCATGCCAGGGGCTAGCCGCTTGATCAGACATCTTCATGCAAAAGGAATACCATGTGCCTTGGCAACGGGTTCTCAGAAGACACATTTTGAATTGAAAACACAAAAACATGGCAAACTTTTTTCATTGATGCATCATCTAGTCCTTGGCGATGATCCAGAAGTTAAACAAGGGAAGCCTGCACCAGATGTATTCCTTGCTGCAGCCAAAAGATTTGAGGGTTCCCCAATTGATCCGAAGAAAATTCTAGTGTTTGAAGATGCACCAGCTGGAGTTCTTGCAGCCAAGAATGCTGGAATGAAGGTTGTAATGGTTCCAGATCCAAGACTGGACAGCTGTTTTTATGATATTGCAGACCAAGTTTTGAAATCCCTATTGGATTTAAACCCAAGCGATTGGGGTCTGCCGCCGTTTGACAATCCGGCGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006066 GO:0006071 GO:0006114 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019400 GO:0019401 GO:0019751 GO:0034637 GO:0042578 GO:0043136 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046173 GO:0071704 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

242

Amino Acids

26.37

Weight (kDa)

6.17

Isoelectric Point (pI)

43.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	16 - 197	3.5e-26	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	17 - 203	1.4e-20	Haloacid dehalogenase-like hydrolase
HAD	PF12710	17 - 158	4.5e-06	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009440)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25840 AT5G57440
fragaria_vesca	FvH4_4g08210
malus_domestica	MD13G1253800.v1.1
prunus_persica	Prupe.1G086000_v2.0.a1
pyrus_communis	pycom13g22440
rosa_chinensis	RchiOBHm_Chr4g0402461 RchiOBHm_Chr6g0266651 RchiOBHm_Chr6g0273661
rosa_laevigata	RLG00000009082
rosa_multiflora	Rmu_co8208732.1_g000001 Rmu_ssc0000330.1_g000027
rosa_roxburghii	Rroxscaffold_4G00321760 Rroxscaffold_5G00347150
rosa_rugosa	Rorug02G0591300 Rorug04G0035600 Rorug05G0169700
rosa_samantha	Rh4AG112900 Rh4BG105400 Rh4CG119800 Rh4DG104700
rosa_wichuraiana	Rw4G009050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	27, 263, 313, 701
AclWI	GGATC	3 cut(s)	443, 521, 608
AcsI	RAATTY	1 cut(s)	537
AcuI	CTGAAG	1 cut(s)	120
AgsI	TTSAA	5 cut(s)	206, 386, 391, 551, 661
AjnI	CCWGG	2 cut(s)	105, 301
AjuI	GAANNNNNNNTTGG	2 cut(s)	653, 685
AluBI	AGCT	6 cut(s)	20, 188, 295, 563, 630, 723
AluI	AGCT	6 cut(s)	20, 188, 295, 563, 630, 723
Alw21I	GWGCWC	1 cut(s)	297
Alw26I	GTCTC	1 cut(s)	153
AlwI	GGATC	3 cut(s)	443, 521, 608
Ama87I	CYCGRG	1 cut(s)	192
AoxI	GGCC	1 cut(s)	67
ApeKI	GCWGC	4 cut(s)	188, 494, 497, 575
ApoI	RAATTY	1 cut(s)	537
AspS9I	GGNCC	1 cut(s)	35
AsuNHI	GCTAGC	1 cut(s)	308
AvaI	CYCGRG	1 cut(s)	192
AvaII	GGWCC	1 cut(s)	35
BanII	GRGCYC	1 cut(s)	297
BbsI	GAAGAC	1 cut(s)	380
Bbv12I	GWGCWC	1 cut(s)	297
BbvCI	CCTCAGC	1 cut(s)	237
BbvI	GCAGC	4 cut(s)	175, 481, 509, 587
BccI	CCATC	1 cut(s)	160
BceAI	ACGGC	2 cut(s)	82, 688
BciT130I	CCWGG	2 cut(s)	107, 303
BclI	TGATCA	1 cut(s)	318
BcoDI	GTCTC	1 cut(s)	153
BfaI	CTAG	5 cut(s)	123, 248, 309, 434, 542
BfmI	CTRYAG	1 cut(s)	495
BisI	GCNGC	7 cut(s)	28, 189, 313, 495, 498, 576, 701
BlsI	GCNGC	7 cut(s)	29, 190, 314, 496, 499, 577, 702
Bme1390I	CCNGG	2 cut(s)	107, 303
Bme18I	GGWCC	1 cut(s)	35
BmeT110I	CYCGRG	1 cut(s)	192
BmgT120I	GGNCC	1 cut(s)	35
BmiI	GGNNCC	2 cut(s)	517, 609
BmrFI	CCNGG	2 cut(s)	107, 303
BmsI	GCATC	3 cut(s)	414, 436, 544
BmtI	GCTAGC	1 cut(s)	312
BpiI	GAAGAC	1 cut(s)	380
BpmI	CTGGAG	1 cut(s)	585
Bpu10I	CCTNAGC	1 cut(s)	237
BsaI	GGTCTC	1 cut(s)	153
BsaJI	CCNNGG	3 cut(s)	302, 354, 439
Bse1I	ACTGG	1 cut(s)	627
BseBI	CCWGG	2 cut(s)	107, 303
BseDI	CCNNGG	3 cut(s)	302, 354, 439
BseMII	CTCAG	2 cut(s)	228, 383
BseNI	ACTGG	1 cut(s)	627
BseXI	GCAGC	4 cut(s)	175, 481, 509, 587
BsgI	GTGCAG	1 cut(s)	459
BshFI	GGCC	1 cut(s)	69
BsiHKAI	GWGCWC	1 cut(s)	297
BsiHKCI	CYCGRG	1 cut(s)	192
BsiSI	CCGG	1 cut(s)	716
BsmAI	GTCTC	1 cut(s)	153
BsmI	GAATGC	1 cut(s)	589
BsnI	GGCC	1 cut(s)	69
Bso31I	GGTCTC	1 cut(s)	153
BsoBI	CYCGRG	1 cut(s)	192
Bsp1286I	GDGCHC	1 cut(s)	297
Bsp143I	GATC	4 cut(s)	318, 448, 526, 613
BspACI	CCGC	4 cut(s)	27, 263, 313, 701
BspANI	GGCC	1 cut(s)	69
BspCNI	CTCAG	2 cut(s)	229, 382
BspLI	GGNNCC	2 cut(s)	517, 609
BspMAI	CTGCAG	1 cut(s)	499
BspOI	GCTAGC	1 cut(s)	312
BspPI	GGATC	3 cut(s)	443, 521, 608
BspTNI	GGTCTC	1 cut(s)	153
BsrI	ACTGG	1 cut(s)	627
BssECI	CCNNGG	3 cut(s)	302, 354, 439
BssMI	GATC	4 cut(s)	318, 448, 526, 613
BssT1I	CCWWGG	2 cut(s)	354, 439
Bst2UI	CCWGG	2 cut(s)	107, 303
BstC8I	GCNNGC	3 cut(s)	310, 474, 721
BstDEI	CTNAG	2 cut(s)	237, 369
BstKTI	GATC	4 cut(s)	321, 451, 529, 616
BstMAI	GTCTC	1 cut(s)	153
BstMBI	GATC	4 cut(s)	318, 448, 526, 613
BstMWI	GCNNNNNNNGC	2 cut(s)	185, 584
BstNI	CCWGG	2 cut(s)	107, 303
BstSCI	CCNGG	2 cut(s)	105, 301
BstSFI	CTRYAG	1 cut(s)	495
BstV1I	GCAGC	4 cut(s)	175, 481, 509, 587
BstV2I	GAAGAC	1 cut(s)	380
BstX2I	RGATCY	1 cut(s)	613
BstXI	CCANNNNNNTGG	1 cut(s)	690
BstYI	RGATCY	1 cut(s)	613
BsuRI	GGCC	1 cut(s)	69
BtgZI	GCGATG	1 cut(s)	459
Cac8I	GCNNGC	3 cut(s)	310, 474, 721
Cfr13I	GGNCC	1 cut(s)	35
CviAII	CATG	5 cut(s)	47, 298, 332, 348, 404
DdeI	CTNAG	2 cut(s)	237, 369
DpnI	GATC	4 cut(s)	320, 450, 528, 615
DpnII	GATC	4 cut(s)	318, 448, 526, 613
DraI	TTTAAA	1 cut(s)	678
Ecl136II	GAGCTC	1 cut(s)	295
Eco130I	CCWWGG	2 cut(s)	354, 439
Eco24I	GRGCYC	1 cut(s)	297
Eco31I	GGTCTC	1 cut(s)	153
Eco47I	GGWCC	1 cut(s)	35
Eco53kI	GAGCTC	1 cut(s)	295
Eco57I	CTGAAG	1 cut(s)	120
Eco88I	CYCGRG	1 cut(s)	192
EcoICRI	GAGCTC	1 cut(s)	295
EcoRII	CCWGG	2 cut(s)	105, 301
EcoT14I	CCWWGG	2 cut(s)	354, 439
EcoT22I	ATGCAT	1 cut(s)	429
EcoT38I	GRGCYC	1 cut(s)	297
ErhI	CCWWGG	2 cut(s)	354, 439
FaeI	CATG	5 cut(s)	50, 301, 335, 351, 407
FaiI	YATR	9 cut(s)	48, 62, 96, 129, 299, 333, 349, 405, 639
FatI	CATG	5 cut(s)	46, 297, 331, 347, 403
FbaI	TGATCA	1 cut(s)	318
Fnu4HI	GCNGC	7 cut(s)	28, 189, 313, 495, 498, 576, 701
FriOI	GRGCYC	1 cut(s)	297
Fsp4HI	GCNGC	7 cut(s)	28, 189, 313, 495, 498, 576, 701
FspBI	CTAG	5 cut(s)	123, 248, 309, 434, 542
GluI	GCNGC	7 cut(s)	28, 189, 313, 495, 498, 576, 701
GsuI	CTGGAG	1 cut(s)	585
HaeIII	GGCC	1 cut(s)	69
HapII	CCGG	1 cut(s)	716
Hin1II	CATG	5 cut(s)	50, 301, 335, 351, 407
HinfI	GANTC	2 cut(s)	7, 209
HpaII	CCGG	1 cut(s)	716
Hpy188I	TCNGA	3 cut(s)	323, 372, 531
Hpy188III	TCNNGA	3 cut(s)	213, 452, 611
HpyAV	CCTTC	1 cut(s)	589
HpyCH4V	TGCA	8 cut(s)	271, 335, 427, 476, 497, 557, 575, 647
HpyF10VI	GCNNNNNNNGC	2 cut(s)	185, 584
HpyF3I	CTNAG	2 cut(s)	237, 369
Hsp92II	CATG	5 cut(s)	50, 301, 335, 351, 407
Ksp22I	TGATCA	1 cut(s)	318
Kzo9I	GATC	4 cut(s)	318, 448, 526, 613
Lsp1109I	GCAGC	4 cut(s)	175, 481, 509, 587
LweI	GCATC	3 cut(s)	414, 436, 544
MaeI	CTAG	5 cut(s)	123, 248, 309, 434, 542
MaeIII	GTNAC	1 cut(s)	221
MalI	GATC	4 cut(s)	320, 450, 528, 615
MboI	GATC	4 cut(s)	318, 448, 526, 613
MboII	GAAGA	6 cut(s)	46, 218, 320, 385, 544, 563
MfeI	CAATTG	1 cut(s)	522
MflI	RGATCY	1 cut(s)	613
MhlI	GDGCHC	1 cut(s)	297
MluCI	AATT	4 cut(s)	216, 386, 522, 537
MmeI	TCCRAC	1 cut(s)	308
MnlI	CCTC	6 cut(s)	22, 26, 80, 232, 253, 505
Mph1103I	ATGCAT	1 cut(s)	429
MseI	TTAA	2 cut(s)	459, 677
MslI	CAYNNNNRTG	1 cut(s)	330
MspA1I	CMGCKG	2 cut(s)	563, 630
MspI	CCGG	1 cut(s)	716
MspR9I	CCNGG	2 cut(s)	107, 303
MunI	CAATTG	1 cut(s)	522
Mva1269I	GAATGC	1 cut(s)	589
MvaI	CCWGG	2 cut(s)	107, 303
MwoI	GCNNNNNNNGC	2 cut(s)	185, 584
NdeII	GATC	4 cut(s)	318, 448, 526, 613
NheI	GCTAGC	1 cut(s)	308
NlaIII	CATG	5 cut(s)	50, 301, 335, 351, 407
NlaIV	GGNNCC	2 cut(s)	517, 609
NmuCI	GTSAC	1 cut(s)	221
NsiI	ATGCAT	1 cut(s)	429
PctI	GAATGC	1 cut(s)	589
PfeI	GAWTC	2 cut(s)	7, 209
PfoI	TCCNGGA	1 cut(s)	105
PkrI	GCNGC	7 cut(s)	29, 190, 314, 496, 499, 577, 702
Psp124BI	GAGCTC	1 cut(s)	297
Psp6I	CCWGG	2 cut(s)	105, 301
PspGI	CCWGG	2 cut(s)	105, 301
PspN4I	GGNNCC	2 cut(s)	517, 609
PspPI	GGNCC	1 cut(s)	35
PstI	CTGCAG	1 cut(s)	499
PsuI	RGATCY	1 cut(s)	613
PvuII	CAGCTG	2 cut(s)	563, 630
RseI	CAYNNNNRTG	1 cut(s)	330
SacI	GAGCTC	1 cut(s)	297
SaqAI	TTAA	2 cut(s)	459, 677
SatI	GCNGC	7 cut(s)	28, 189, 313, 495, 498, 576, 701
Sau3AI	GATC	4 cut(s)	318, 448, 526, 613
Sau96I	GGNCC	1 cut(s)	35
ScrFI	CCNGG	2 cut(s)	107, 303
SduI	GDGCHC	1 cut(s)	297
SetI	ASST	8 cut(s)	22, 128, 190, 297, 565, 600, 632, 725
SfaNI	GCATC	3 cut(s)	414, 436, 544
SfcI	CTRYAG	1 cut(s)	495
SinI	GGWCC	1 cut(s)	35
SmiMI	CAYNNNNRTG	1 cut(s)	330
Sse9I	AATT	4 cut(s)	216, 386, 522, 537
SsiI	CCGC	4 cut(s)	27, 263, 313, 701
SspMI	CTAG	5 cut(s)	123, 248, 309, 434, 542
SstI	GAGCTC	1 cut(s)	297
StyD4I	CCNGG	2 cut(s)	105, 301
StyI	CCWWGG	2 cut(s)	354, 439
TaqI	TCGA	3 cut(s)	57, 78, 183
TasI	AATT	4 cut(s)	216, 386, 522, 537
TauI	GCSGC	3 cut(s)	30, 315, 703
TfiI	GAWTC	2 cut(s)	7, 209
Tru1I	TTAA	2 cut(s)	459, 677
Tru9I	TTAA	2 cut(s)	459, 677
TseFI	GTSAC	1 cut(s)	221
TseI	GCWGC	4 cut(s)	188, 494, 497, 575
Tsp45I	GTSAC	1 cut(s)	221
TspDTI	ATGAA	3 cut(s)	320, 408, 608
VpaK11BI	GGWCC	1 cut(s)	35
XapI	RAATTY	1 cut(s)	537
XspI	CTAG	5 cut(s)	123, 248, 309, 434, 542
Zsp2I	ATGCAT	1 cut(s)	429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.