Rorug05G0169700

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009440
Physical Location & Seq
Reverse (-)
15767236 .. 15782176
14941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0169700.1

Sequence Viewer

Length: 3783 bp
ATGGCGCAGCAGCAGCAGCAGCAGCAGCCTCCGTCGGTCCCGCCGCAATTGCCGACCGCCGGCGCCAGCGGGCCCGTCGGAGGCGTCCAGGTGCGGTGCGCCGGCTGCCACAAGATCCTCACCATCGCGGCCGGGGTCACCGAATTCGTCTGCGGCACGTGTCAATTGCCGCAGATGCTTCCGCCGGAGCTGATGAGCCGGGCCCAGGCCCATAACCAGCCCAACAAGGGCCCGCTTCCCCTGCCCCCGCCGCGGCCGCAGCCGCAGCACGTGGCCGCCCACGGCGTCGATCCTTCGAAGATCCAGCTCCCCTGCGCCAACTGCAAGGCGATTCTCAATGTGCCTCATGGACTCTCTCGGTTTCAGTGCCCTCAGTGCCATGTTGACCTCGCCGTCGATGTGTCCAAGCTCAAAGAGTTCTTGCTGCCACCACTGCCTTCCGAGGAGGTCAACGAGGTTGCCATTGAAGTGGAGCGAGAAGAAGATGAAGGTGGCCTGGCCGGGGAAACATTTACAGATTATCGCCCTCCAAAGCTGTCCATTGGACCTCCTCATCCAGATCCCGTTGTGGAAACATCATCCCTGTCGGCTGTGCAGCCACCTGAGCCCACTTACGACCTAACAATCAAGGATGATTTGGAAAATTCAAAGGCTTTATCATGTTTGCAGATTGAGACATTGGTTTATGCCTGTCAGAGACACCTCCAGCACCTTCCAAGTGGTGTTAGGGCTGGATTCTTTGTTGGAGATGGAGCTGGTGTGGGTAAAGGTCGAACAATTGCAGGGTTAATTTGGGAAAATTGGCACCATGGGAGGAGAAAGGCAGTGTGGATTTCTGTTGGTTCAGACTTGAAGTTTGATGCAAGAAGAGACTTAGATGATGTAGGCGCAACTTGTATAGAAGTTCATGCTTTGAACAAGCTGCCATATTCTAAGCTTGATTCCAAGTCTGTTGGCATCAAGGAGGGAGTTATATTCCTCACATACAGCAGCCTTATAGCATCTTCTGAGAAAGGTCGTTCACGCATGCAGCAGCTTGTGCAGTGGTGTGGATCAGGATATGATGGTCTTATCGTCTTTGACGAGTGTCACAAAGCCAAAAATTTGATACCTGAAGCTGGAAGTCAGCCAACACGAACCGGTGAAGCAGTTCTTGACATTCAGGCTCGACTGCCTGAAGCTCGGGTTATTTATTGCTCGGCAACTGGTGCATCAGAACCACGCAACTTGGGTTACATGGTTCGGCTTGGTCTTTGGGGCCCTGGAACATCTTTTTCTGAGTTCCGTGAATTTCTAGCTGCTCTTGAGAAAGGAGGTGTTGGTGCACTTGAACTTGTTGCCATGGACATGAAAGCAAGGGGGATGTATGTCTGTCGTACTCTTAGCTATAAAGGAGCAGAGTTTGACGTTGTTGAGGCACCACTAGAAGATGAAATGATGGAAATGTACAAAAAAGCAGCAGAATTTTGGACAGAGCTGCGAGTCGAAATACTAACAGCTACTGCCTATCTCACTAATGAAAAGCCCATTTCTAGTCAAGTCTGGCGATTGTATTGGGCTAGTCATCAGCGTTTCTTTAGACATATGTGTATGTCGGCTAAGGTTCCTGCTGCTGTAAGACTAGCGAAGAAAGCATTGATGGATGACAAGTGTGTCGTAATTGGCCTTCAGAGTACTGGAGAGGCACGAACTGAAGAAGCTGTGACTAAATATGGTCTTGAGCTTGATGATTTTATTTCGGGACCTCGAGAACTGTTGCTGAAATTTGTTGAAGAAAACTATCCTTTGCCTGAAAAGCCTGATCCTGTCACAGGAGAAGAAAGTGTAAAGGAGCTTCAACGGAAGAGGCACTCAGCAAGTCCTGGTGTTTCGATGAAAGGGAGAGTGAGGAAAGTTGCAAAAGTGCAACCGCCAATTGATAACGAAAGTGACGAAGAATCTGAAACTGATTCTGCTGTTGAATCTACTGAATCAGATGACGAGTTTCAAATCTGTGAAATCTGCAATGCCGAAACGGAAAGAAAGAAATTGCTCCACTGTTCTTGTTGTGGCCAACTGGTCCATGCTGAATGCCTTATCCCACCTGTGATAGATGAGGTATCAGAAGATTGGTCATGTCATTCGTGCAAGGAAAAAACAGATGAATATCTTCAACGGAGAGAGCAGTATATAGCTGAATTGAAGAAGAGGTATGAAGCAGCTTTGGAGCGTAAGTCAAAAATTCTGGGGATAATTCGTTCCTTGGATCTTCCAAACAATCCTTTGGACGATATCATTGATCAGCTTGGAGGCCCTGATAAAGTAGCTGAAATGACAGGAAGGCGAGGTATGCTTGTGAGGGCATCTAATGGTAAAGGTGTTACCTATCAGGCTCGAAACACGAAGGAGGTTTCCATGGAGATGGTCAACATGCATGAGAAACAGCTTTTCATGGATGGCAAAAAGTTTGTTGCTATAATTTCTGAGGCTGGGTCAGCTGGTGTTTCTTTGCAGGCAGATAGAAGAGCTGTAAATCAGAAAAGAAGGGTACATCTAACCCTGGAACTTCCTTGGAGTGCCGATCGAGCGATTCAACAGTTTGGAAGAACTCACCGGTCAAATCAAGCTTCTGCTCCTGAATACAGGCTACTCTTTACTAACCTCGGTGGAGAACGTCGGTTTGCATCCATTGTTGCAAAGAGATTACAATCTCTTGGAGCTCTAACTCAGGGTGATCGCAGGGCAGGCCCATCATTGAGTGCCTATAATTATGATAGTGCTTATGGAAAGAAGGCATTAATATTGATGTATAGAGGAATATTGGAGCAGGAAGCTCTGCCTGTTGTACCACCAGGATGTTCATCTGAAGACCCGGAGACAATCCAAGATTTTATTGAGGAGGCAAGAGCTGCTCTTGTGTTTGTAGGAATAATTAGGGATGCTAAAGATTCTGGCAAGCTCTCTGGCCGTGTAGCTGATTCAGACATGCATGACGTCGGACGTTTCCTCAATCGGATTTTAGGATTGCCACCTAAAGTACAAAATAGGCTGTTCGAGTTATTTGTTAGTATTTTGGATCTTCTTGTCCATAATGCACGCATTGAAGGAAATCTTGACTCTGGGATTGTTGATATGAAAGCTAATGTTATAGAGCTACAAGGAACTCCAAAGACTGTCCATGTTGACGAAATGTCTGGAGCTTCAACTGTGCTCTTCACTTTTACCCTGGATCGTGGCATCACATGGGAGTCTGCAAGTGCCATGCTTGAAGGTAAACAAGAGGAGGGGCTTAGTTGTGCTCATGATGGCTTCTATGAATCTAGAAGGGAGTGGATGGGGAGGCGCCATACCATATTAGCCTTTGAAAGTTCTACTTCTGGGTCCTATAAGATAGTCCGTCCGGCAGTTGGAGAGTCGGTGAGAGAAATGTCTCTGTCTGAGCTAAAAAGCAAATACAGAAGAACATCATCGTTGGAGAAGGCTCGCAGCGGTTGGAATGATGAATATGAAGTTTCATCCAAACAGTGCATGCATGGTCCCAAGTGTAAGGTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGTCTCATTCTTCCTGTATGGGGCACTATTGAGAAAGCTCTTTCTAAGCAGTCCCGGCAAAGCCACAGGCGGCTACGTGTTGTGCGTATAGAAACCACAACAGATAACCAGAGAATCGTTGGTCTGTTTGTCCCCAATGCAGCTGTTGAAGCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATTGAAGATTCACAGTAG

Protein Analysis

1260

Amino Acids

139.55

Weight (kDa)

6.15

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_FGT1_1 PF23547 29 - 63 3.5e-18 FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1 PF23547 101 - 130 6.4e-10 FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2 PF23548 101 - 134 3.8e-17 FORGETTER1 second zinc ribbon domain
AAA_34 PF13872 184 - 484 1e-134 P-loop containing NTP hydrolase pore-1
Helicase_C_4 PF13871 753 - 1037 2.5e-104 C-terminal domain on Strawberry notch homologue
SBNO PF25373 1074 - 1186 2.6e-18 SBNO alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 392, 1652
AatII GACGTC 1 cut(s) 2976
AccB1I GGYRCC 4 cut(s) 62, 804, 1417, 3321
AccII CGCG 2 cut(s) 128, 253
AclWI GGATC 9 cut(s) 109, 284, 295, 554, 1060, 1796, 2253, 3063, 3216
AcoI YGGCCR 6 cut(s) 129, 254, 273, 498, 2050, 2944
AcsI RAATTY 7 cut(s) 143, 643, 1102, 1289, 1463, 1763, 2220
AcuI CTGAAG 5 cut(s) 1134, 1197, 1652, 1713, 2865
AcvI CACGTG 2 cut(s) 159, 271
AcyI GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
AfaI GTAC 8 cut(s) 1378, 1448, 1675, 2529, 2826, 3018, 3544, 3738
AflIII ACRYGT 2 cut(s) 158, 3658
AgeI ACCGGT 2 cut(s) 1139, 2592
AhdI GACNNNNNGTC 1 cut(s) 3169
AjnI CCWGG 8 cut(s) 87, 204, 495, 1261, 1861, 2538, 2830, 3204
AloI GAACNNNNNNTCC 2 cut(s) 2221, 2253
Alw21I GWGCWC 4 cut(s) 1327, 2701, 3192, 3280
Alw26I GTCTC 6 cut(s) 668, 691, 864, 2849, 3414, 3590
Alw44I GTGCAC 1 cut(s) 1323
AlwI GGATC 9 cut(s) 109, 284, 295, 554, 1060, 1796, 2253, 3063, 3216
AlwNI CAGNNNCTG 1 cut(s) 1502
Ama87I CYCGRG 2 cut(s) 1182, 1746
ApaI GGGCCC 4 cut(s) 75, 205, 233, 1262
ApaLI GTGCAC 1 cut(s) 1323
ApoI RAATTY 7 cut(s) 143, 643, 1102, 1289, 1463, 1763, 2220
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
AseI ATTAAT 1 cut(s) 2777
AsiGI ACCGGT 2 cut(s) 1139, 2592
Asp700I GAANNNNTTC 2 cut(s) 1149, 2148
AspLEI GCGC 6 cut(s) 7, 65, 101, 317, 890, 3324
AsuC2I CCSGG 5 cut(s) 133, 200, 502, 2852, 3637
AsuHPI GGTGA 6 cut(s) 112, 130, 1154, 2582, 2723, 3409
AsuII TTCGAA 1 cut(s) 296
AvaI CYCGRG 2 cut(s) 1182, 1746
AvaII GGWCC 6 cut(s) 37, 545, 1742, 2059, 3360, 3515
BaeGI GKGCMC 7 cut(s) 75, 205, 233, 371, 1262, 1327, 3608
BalI TGGCCA 1 cut(s) 2052
BanI GGYRCC 4 cut(s) 62, 804, 1417, 3321
BanII GRGCYC 6 cut(s) 75, 205, 233, 609, 1262, 2701
BarI GAAGNNNNNNTAC 4 cut(s) 1650, 1682, 1818, 1850
BbrPI CACGTG 2 cut(s) 159, 271
BbsI GAAGAC 2 cut(s) 2853, 3562
Bbv12I GWGCWC 4 cut(s) 1327, 2701, 3192, 3280
BceAI ACGGC 4 cut(s) 298, 377, 2931, 3719
BciT130I CCWGG 8 cut(s) 89, 206, 497, 1263, 1863, 2540, 2832, 3206
BclI TGATCA 1 cut(s) 2278
BcnI CCSGG 5 cut(s) 133, 200, 502, 2852, 3637
BcoDI GTCTC 6 cut(s) 668, 691, 864, 2849, 3414, 3590
BfaI CTAG 6 cut(s) 1295, 1424, 1533, 1560, 1622, 3300
BfmI CTRYAG 1 cut(s) 3560
BfoI RGCGCY 2 cut(s) 66, 3325
BmcAI AGTACT 1 cut(s) 1675
Bme18I GGWCC 6 cut(s) 37, 545, 1742, 2059, 3360, 3515
BmeRI GACNNNNNGTC 1 cut(s) 3169
BmeT110I CYCGRG 2 cut(s) 1182, 1746
BmsI GCATC 9 cut(s) 165, 850, 966, 1010, 1220, 2351, 2672, 2908, 3225
BoxI GACNNNNGTC 1 cut(s) 1086
BpiI GAAGAC 2 cut(s) 2853, 3562
BpmI CTGGAG 3 cut(s) 689, 1698, 3195
Bpu10I CCTNAGC 2 cut(s) 603, 1599
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 1325, 1739
BpuMI CCSGG 5 cut(s) 133, 200, 502, 2852, 3637
BsaAI YACGTR 3 cut(s) 159, 271, 3659
BsaBI GATNNNNATC 3 cut(s) 2145, 2686, 2839
BsaHI GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
BsaI GGTCTC 1 cut(s) 3590
BsaWI WCCGGW 2 cut(s) 1139, 2592
BsaXI ACNNNNNCTCC 6 cut(s) 744, 774, 2198, 2228, 3381, 3411
Bse118I RCCGGY 4 cut(s) 59, 101, 1139, 2592
Bse1I ACTGG 3 cut(s) 1210, 1681, 2061
Bse3DI GCAATG 1 cut(s) 2011
Bse8I GATNNNNATC 3 cut(s) 2145, 2686, 2839
BseBI CCWGG 8 cut(s) 89, 206, 497, 1263, 1863, 2540, 2832, 3206
BseJI GATNNNNATC 3 cut(s) 2145, 2686, 2839
BseMI GCAATG 1 cut(s) 2011
BseMII CTCAG 8 cut(s) 386, 594, 999, 1269, 1866, 2454, 2720, 3408
BseNI ACTGG 3 cut(s) 1210, 1681, 2061
BseRI GAGGAG 5 cut(s) 458, 540, 829, 2891, 3275
BseSI GKGCMC 7 cut(s) 75, 205, 233, 371, 1262, 1327, 3608
BseX3I CGGCCG 2 cut(s) 129, 254
BseYI CCCAGC 1 cut(s) 2468
BsgI GTGCAG 2 cut(s) 614, 1061
Bsh1236I CGCG 2 cut(s) 128, 253
Bsh1285I CGRYCG 4 cut(s) 57, 132, 257, 2563
BshNI GGYRCC 4 cut(s) 62, 804, 1417, 3321
BshTI ACCGGT 2 cut(s) 1139, 2592
BsiEI CGRYCG 4 cut(s) 57, 132, 257, 2563
BsiHKAI GWGCWC 4 cut(s) 1327, 2701, 3192, 3280
BsiHKCI CYCGRG 2 cut(s) 1182, 1746
BslFI GGGAC 5 cut(s) 23, 1755, 3501, 3619, 3698
BsmAI GTCTC 6 cut(s) 668, 691, 864, 2849, 3414, 3590
BsmFI GGGAC 5 cut(s) 23, 1755, 3501, 3619, 3698
BsmI GAATGC 1 cut(s) 2075
Bso31I GGTCTC 1 cut(s) 3590
BsoBI CYCGRG 2 cut(s) 1182, 1746
Bsp119I TTCGAA 1 cut(s) 296
Bsp120I GGGCCC 4 cut(s) 71, 201, 229, 1258
Bsp1407I TGTACA 1 cut(s) 1446
Bsp19I CCATGG 3 cut(s) 808, 1341, 2394
BspCNI CTCAG 8 cut(s) 385, 595, 1000, 1270, 1865, 2455, 2719, 3409
BspFNI CGCG 2 cut(s) 128, 253
BspHI TCATGA 1 cut(s) 3280
BspMAI CTGCAG 1 cut(s) 3564
BspPI GGATC 9 cut(s) 109, 284, 295, 554, 1060, 1796, 2253, 3063, 3216
BspQI GCTCTTC 2 cut(s) 2497, 3197
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 4 cut(s) 62, 804, 1417, 3321
BspTNI GGTCTC 1 cut(s) 3590
BsrDI GCAATG 1 cut(s) 2011
BsrFI RCCGGY 4 cut(s) 59, 101, 1139, 2592
BsrGI TGTACA 1 cut(s) 1446
BsrI ACTGG 3 cut(s) 1210, 1681, 2061
BssAI RCCGGY 4 cut(s) 59, 101, 1139, 2592
BssNI GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
BssT1I CCWWGG 5 cut(s) 808, 1341, 2241, 2394, 2549
Bst2UI CCWGG 8 cut(s) 89, 206, 497, 1263, 1863, 2540, 2832, 3206
Bst4CI ACNGT 8 cut(s) 1755, 2039, 2577, 3154, 3187, 3504, 3547, 3780
Bst6I CTCTTC 5 cut(s) 862, 1838, 2180, 2497, 3197
BstACI GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
BstAPI GCANNNNNTGC 3 cut(s) 1039, 1208, 2888
BstAUI TGTACA 1 cut(s) 1446
BstBAI YACGTR 3 cut(s) 159, 271, 3659
BstBI TTCGAA 1 cut(s) 296
BstDSI CCRYGG 5 cut(s) 251, 280, 808, 1341, 2394
BstEII GGTNACC 1 cut(s) 136
BstENI CCTNNNNNAGG 1 cut(s) 1809
BstFNI CGCG 2 cut(s) 128, 253
BstH2I RGCGCY 2 cut(s) 66, 3325
BstHHI GCGC 6 cut(s) 7, 65, 101, 317, 890, 3324
BstMAI GTCTC 6 cut(s) 668, 691, 864, 2849, 3414, 3590
BstMCI CGRYCG 4 cut(s) 57, 132, 257, 2563
BstNI CCWGG 8 cut(s) 89, 206, 497, 1263, 1863, 2540, 2832, 3206
BstNSI RCATGY 4 cut(s) 1030, 2413, 2968, 3511
BstPAI GACNNNNGTC 1 cut(s) 1086
BstPI GGTNACC 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 3560
BstSLI GKGCMC 7 cut(s) 75, 205, 233, 371, 1262, 1327, 3608
BstUI CGCG 2 cut(s) 128, 253
BstV2I GAAGAC 2 cut(s) 2853, 3562
BstX2I RGATCY 5 cut(s) 114, 300, 559, 2245, 3055
BstXI CCANNNNNNTGG 2 cut(s) 469, 2401
BstYI RGATCY 5 cut(s) 114, 300, 559, 2245, 3055
BstZI CGGCCG 2 cut(s) 129, 254
BtgI CCRYGG 5 cut(s) 251, 280, 808, 1341, 2394
BtgZI GCGATG 1 cut(s) 109
BtsI GCAGTG 3 cut(s) 431, 831, 1049
BtsIMutI CAGTG 7 cut(s) 371, 380, 431, 831, 1049, 2035, 3509
CaiI CAGNNNCTG 1 cut(s) 1502
CciI TCATGA 1 cut(s) 3280
CciNI GCGGCCGC 1 cut(s) 254
CfoI GCGC 6 cut(s) 7, 65, 101, 317, 890, 3324
Cfr10I RCCGGY 4 cut(s) 59, 101, 1139, 2592
Cfr42I CCGCGG 1 cut(s) 254
CseI GACGC 2 cut(s) 73, 274
Csp6I GTAC 8 cut(s) 1377, 1447, 1674, 2528, 2825, 3017, 3543, 3737
CspAI ACCGGT 2 cut(s) 1139, 2592
CspCI CAANNNNNGTGG 4 cut(s) 1209, 1244, 2024, 2059
CviQI GTAC 8 cut(s) 1377, 1447, 1674, 2528, 2825, 3017, 3543, 3737
DinI GGCGCC 2 cut(s) 64, 3323
DrdI GACNNNNNNGTC 2 cut(s) 392, 1652
DriI GACNNNNNGTC 1 cut(s) 3169
DseDI GACNNNNNNGTC 2 cut(s) 392, 1652
EaeI YGGCCR 6 cut(s) 129, 254, 273, 498, 2050, 2944
EagI CGGCCG 2 cut(s) 129, 254
Eam1104I CTCTTC 5 cut(s) 862, 1838, 2180, 2497, 3197
Eam1105I GACNNNNNGTC 1 cut(s) 3169
EarI CTCTTC 5 cut(s) 862, 1838, 2180, 2497, 3197
EciI GGCGGA 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 2699
EclXI CGGCCG 2 cut(s) 129, 254
Eco130I CCWWGG 5 cut(s) 808, 1341, 2241, 2394, 2549
Eco24I GRGCYC 6 cut(s) 75, 205, 233, 609, 1262, 2701
Eco31I GGTCTC 1 cut(s) 3590
Eco32I GATATC 1 cut(s) 2272
Eco47I GGWCC 6 cut(s) 37, 545, 1742, 2059, 3360, 3515
Eco52I CGGCCG 2 cut(s) 129, 254
Eco53kI GAGCTC 1 cut(s) 2699
Eco57I CTGAAG 5 cut(s) 1134, 1197, 1652, 1713, 2865
Eco72I CACGTG 2 cut(s) 159, 271
Eco88I CYCGRG 2 cut(s) 1182, 1746
Eco91I GGTNACC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 2699
EcoNI CCTNNNNNAGG 1 cut(s) 1809
EcoO109I RGGNCCY 6 cut(s) 229, 1258, 1259, 1742, 2291, 3360
EcoO65I GGTNACC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 143
EcoRII CCWGG 8 cut(s) 87, 204, 495, 1261, 1861, 2538, 2830, 3204
EcoRV GATATC 1 cut(s) 2272
EcoT14I CCWWGG 5 cut(s) 808, 1341, 2241, 2394, 2549
EcoT22I ATGCAT 3 cut(s) 2415, 2970, 3513
EcoT38I GRGCYC 6 cut(s) 75, 205, 233, 609, 1262, 2701
EgeI GGCGCC 2 cut(s) 64, 3323
EheI GGCGCC 2 cut(s) 64, 3323
ErhI CCWWGG 5 cut(s) 808, 1341, 2241, 2394, 2549
FalI AAGNNNNNCTT 8 cut(s) 1137, 1169, 3075, 3107, 3337, 3369, 3723, 3755
FaqI GGGAC 5 cut(s) 23, 1755, 3501, 3619, 3698
FauI CCCGC 4 cut(s) 48, 62, 240, 255
FauNDI CATATG 1 cut(s) 1584
FbaI TGATCA 1 cut(s) 2278
FriOI GRGCYC 6 cut(s) 75, 205, 233, 609, 1262, 2701
FspBI CTAG 6 cut(s) 1295, 1424, 1533, 1560, 1622, 3300
GlaI GCGC 6 cut(s) 6, 64, 100, 316, 889, 3323
GsaI CCCAGC 1 cut(s) 2472
GsuI CTGGAG 3 cut(s) 689, 1698, 3195
HaeII RGCGCY 2 cut(s) 66, 3325
HgaI GACGC 2 cut(s) 73, 274
HhaI GCGC 6 cut(s) 7, 65, 101, 317, 890, 3324
Hin1I GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
Hin6I GCGC 6 cut(s) 5, 63, 99, 315, 888, 3322
HinP1I GCGC 6 cut(s) 5, 63, 99, 315, 888, 3322
HincII GTYRAC 4 cut(s) 385, 451, 2407, 3163
HindII GTYRAC 4 cut(s) 385, 451, 2407, 3163
HindIII AAGCTT 2 cut(s) 935, 2604
HphI GGTGA 6 cut(s) 112, 130, 1154, 2582, 2723, 3409
Hpy166II GTNNAC 7 cut(s) 385, 451, 1022, 1325, 2407, 3163, 3254
Hpy8I GTNNAC 7 cut(s) 385, 451, 1022, 1325, 2407, 3163, 3254
Hpy99I CGWCG 6 cut(s) 37, 80, 290, 398, 2658, 2978
HpyCH4III ACNGT 8 cut(s) 1755, 2039, 2577, 3154, 3187, 3504, 3547, 3780
HpyCH4IV ACGT 7 cut(s) 158, 270, 1407, 2653, 2973, 2980, 3658
HpySE526I ACGT 7 cut(s) 158, 270, 1407, 2653, 2973, 2980, 3658
Hsp92I GRCGYC 5 cut(s) 63, 84, 285, 2973, 3322
HspAI GCGC 6 cut(s) 5, 63, 99, 315, 888, 3322
KasI GGCGCC 2 cut(s) 62, 3321
KroI GCCGGC 2 cut(s) 59, 101
KroNI GCCGGC 2 cut(s) 61, 103
Ksp22I TGATCA 1 cut(s) 2278
KspI CCGCGG 1 cut(s) 254
LguI GCTCTTC 2 cut(s) 2497, 3197
LweI GCATC 9 cut(s) 165, 850, 966, 1010, 1220, 2351, 2672, 2908, 3225
MaeI CTAG 6 cut(s) 1295, 1424, 1533, 1560, 1622, 3300
MaeII ACGT 7 cut(s) 158, 270, 1407, 2653, 2973, 2980, 3658
MaeIII GTNAC 7 cut(s) 136, 1088, 1232, 1702, 1807, 1928, 2359
MfeI CAATTG 4 cut(s) 47, 164, 777, 1914
MflI RGATCY 5 cut(s) 114, 300, 559, 2245, 3055
MlsI TGGCCA 1 cut(s) 2052
MluNI TGGCCA 1 cut(s) 2052
Mly113I GGCGCC 2 cut(s) 63, 3322
MlyI GAGTC 5 cut(s) 345, 1491, 3089, 3236, 3401
MmeI TCCRAC 6 cut(s) 58, 724, 2956, 3367, 3432, 3452
Mox20I TGGCCA 1 cut(s) 2052
Mph1103I ATGCAT 3 cut(s) 2415, 2970, 3513
MreI CGCCGGCG 1 cut(s) 59
MroNI GCCGGC 2 cut(s) 59, 101
MroXI GAANNNNTTC 2 cut(s) 1149, 2148
MscI TGGCCA 1 cut(s) 2052
MseI TTAA 2 cut(s) 788, 2777
MslI CAYNNNNRTG 4 cut(s) 467, 1346, 2399, 2833
Msp20I TGGCCA 1 cut(s) 2052
MspA1I CMGCKG 5 cut(s) 69, 253, 2477, 3468, 3725
MunI CAATTG 4 cut(s) 47, 164, 777, 1914
Mva1269I GAATGC 1 cut(s) 2075
MvaI CCWGG 8 cut(s) 89, 206, 497, 1263, 1863, 2540, 2832, 3206
MvnI CGCG 2 cut(s) 128, 253
NaeI GCCGGC 2 cut(s) 61, 103
NarI GGCGCC 2 cut(s) 63, 3322
NciI CCSGG 5 cut(s) 133, 200, 502, 2852, 3637
NcoI CCATGG 3 cut(s) 808, 1341, 2394
NdeI CATATG 1 cut(s) 1584
NgoMIV GCCGGC 2 cut(s) 59, 101
NmeAIII GCCGAG 1 cut(s) 1178
NmuCI GTSAC 5 cut(s) 136, 1088, 1702, 1807, 1928
NotI GCGGCCGC 1 cut(s) 254
NsiI ATGCAT 3 cut(s) 2415, 2970, 3513
NspI RCATGY 4 cut(s) 1030, 2413, 2968, 3511
NspV TTCGAA 1 cut(s) 296
PaeI GCATGC 2 cut(s) 1030, 3511
PaeR7I CTCGAG 1 cut(s) 1746
PagI TCATGA 1 cut(s) 3280
PciSI GCTCTTC 2 cut(s) 2497, 3197
PcsI WCGNNNNNNNCGW 3 cut(s) 1080, 1929, 3664
PctI GAATGC 1 cut(s) 2075
PdiI GCCGGC 2 cut(s) 61, 103
PdmI GAANNNNTTC 2 cut(s) 1149, 2148
PinAI ACCGGT 2 cut(s) 1139, 2592
Ple19I CGATCG 1 cut(s) 2563
PleI GAGTC 5 cut(s) 345, 1490, 3089, 3235, 3400
PluTI GGCGCC 2 cut(s) 66, 3325
PmaCI CACGTG 2 cut(s) 159, 271
PmlI CACGTG 2 cut(s) 159, 271
PpsI GAGTC 5 cut(s) 345, 1490, 3089, 3235, 3400
Ppu21I YACGTR 3 cut(s) 159, 271, 3659
PpuMI RGGWCCY 2 cut(s) 1742, 3360
PshAI GACNNNNGTC 1 cut(s) 1086
PshBI ATTAAT 1 cut(s) 2777
Psp124BI GAGCTC 1 cut(s) 2701
Psp5II RGGWCCY 2 cut(s) 1742, 3360
Psp6I CCWGG 8 cut(s) 87, 204, 495, 1261, 1861, 2538, 2830, 3204
PspCI CACGTG 2 cut(s) 159, 271
PspEI GGTNACC 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 2468
PspGI CCWGG 8 cut(s) 87, 204, 495, 1261, 1861, 2538, 2830, 3204
PspOMI GGGCCC 4 cut(s) 71, 201, 229, 1258
PspPPI RGGWCCY 2 cut(s) 1742, 3360
PstI CTGCAG 1 cut(s) 3564
PstNI CAGNNNCTG 1 cut(s) 1502
PsuI RGATCY 5 cut(s) 114, 300, 559, 2245, 3055
PvuI CGATCG 1 cut(s) 2563
PvuII CAGCTG 2 cut(s) 2477, 3725
RsaI GTAC 8 cut(s) 1378, 1448, 1675, 2529, 2826, 3018, 3544, 3738
RsaNI GTAC 8 cut(s) 1377, 1447, 1674, 2528, 2825, 3017, 3543, 3737
RseI CAYNNNNRTG 4 cut(s) 467, 1346, 2399, 2833
SacI GAGCTC 1 cut(s) 2701
SacII CCGCGG 1 cut(s) 254
SapI GCTCTTC 2 cut(s) 2497, 3197
SaqAI TTAA 2 cut(s) 788, 2777
ScaI AGTACT 1 cut(s) 1675
SchI GAGTC 5 cut(s) 345, 1491, 3089, 3236, 3401
SfaNI GCATC 9 cut(s) 165, 850, 966, 1010, 1220, 2351, 2672, 2908, 3225
SfcI CTRYAG 1 cut(s) 3560
SfoI GGCGCC 2 cut(s) 64, 3323
Sfr274I CTCGAG 1 cut(s) 1746
Sfr303I CCGCGG 1 cut(s) 254
SfuI TTCGAA 1 cut(s) 296
SgrAI CRCCGGYG 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 254
SinI GGWCC 6 cut(s) 37, 545, 1742, 2059, 3360, 3515
SlaI CTCGAG 1 cut(s) 1746
SmiMI CAYNNNNRTG 4 cut(s) 467, 1346, 2399, 2833
SmlI CTYRAG 3 cut(s) 1304, 1718, 1746
SmoI CTYRAG 3 cut(s) 1304, 1718, 1746
SphI GCATGC 2 cut(s) 1030, 3511
SspDI GGCGCC 2 cut(s) 62, 3321
SspI AATATT 2 cut(s) 2781, 2799
SspMI CTAG 6 cut(s) 1295, 1424, 1533, 1560, 1622, 3300
SstI GAGCTC 1 cut(s) 2701
StyI CCWWGG 5 cut(s) 808, 1341, 2241, 2394, 2549
TaaI ACNGT 8 cut(s) 1755, 2039, 2577, 3154, 3187, 3504, 3547, 3780
TaiI ACGT 7 cut(s) 161, 273, 1410, 2656, 2976, 2983, 3661
TaqII GACCGA 1 cut(s) 25
TatI WGTACW 4 cut(s) 1446, 1673, 3016, 3542
Tru1I TTAA 2 cut(s) 788, 2777
Tru9I TTAA 2 cut(s) 788, 2777
TscAI CASTG 7 cut(s) 371, 380, 438, 831, 1049, 2042, 3509
TseFI GTSAC 5 cut(s) 136, 1088, 1702, 1807, 1928
Tsp45I GTSAC 5 cut(s) 136, 1088, 1702, 1807, 1928
TspGWI ACGGA 6 cut(s) 21, 1274, 1855, 2030, 2170, 3365
TspRI CASTG 7 cut(s) 371, 380, 438, 831, 1049, 2042, 3509
VneI GTGCAC 1 cut(s) 1323
VpaK11BI GGWCC 6 cut(s) 37, 545, 1742, 2059, 3360, 3515
VspI ATTAAT 1 cut(s) 2777
XagI CCTNNNNNAGG 1 cut(s) 1809
XapI RAATTY 7 cut(s) 143, 643, 1102, 1289, 1463, 1763, 2220
XbaI TCTAGA 1 cut(s) 3299
XceI RCATGY 4 cut(s) 1030, 2413, 2968, 3511
XcmI CCANNNNNNNNNTGG 1 cut(s) 3698
XhoI CTCGAG 1 cut(s) 1746
XmnI GAANNNNTTC 2 cut(s) 1149, 2148
XspI CTAG 6 cut(s) 1295, 1424, 1533, 1560, 1622, 3300
ZraI GACGTC 1 cut(s) 2974
ZrmI AGTACT 1 cut(s) 1675
Zsp2I ATGCAT 3 cut(s) 2415, 2970, 3513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.