Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002316

Physical Location & Seq

Genomic Coordinates Reverse (-)

34816649 .. 34824000

Gene Structure 7352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1267100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGTCTGGTAAAGGAAGGGGTTGGTCTGATTCAGCACCACTACTTGCCCACCAACAATCCCAATCACAGAAGCAGCACCAGCAACAACAGCATAATCTCCGTCAGTTTCAGGGTCGCTCCTCTTCAAATTCTCACTCCTCAGTCTCTCTCGAGATGACCTCCTCCGCCGTCCCAACCGCCACCGCCCGCTCCGTCTTCCTCGGCATAGATGTTGGCACCGGTAGCGCCCGCGCAGGTCTGTTCGACGAGGACGGGAAACTTCTAGGTTCTTCGAGCAGTTCGATTCAGATTTGGAAAGATGGCGATTCTGTTGAGCAATCGTCCACAGATATTTGGCATGCAATAAGTGCGGCTGTGAAAGCAGCGTGCTCCCTTGCAAAAGTTTCCAAGGAAGAAGTGAAGGGTATGGGATTTGCGGCTACTTGTTCACTTGTTGCAGTGGATGCTGACGGCTCTCCTGTTACGGTTTCTTGGAGTGGTGATTCAAGAAGAAACATAATAGTATGGATGGACCATAGAGCTGTTGAACAAGCTGAAAGGATTAATTCACGTAACTCAACCGTATTGCAGTACTGTGGTGGAGCTCTTTCCCCTGAGATGCAGCCTCCAAAGCTTCTATGGGTGAAAGAAAATCTTCCTGAGTCTTGGTCAATGGTCTTCAGGTGGATGGACCTGAGTGATTGGTTATCATACAGGGCCACAGGAGATGATACTCGGAGTCTCTGTACGACAGTGTGCAAGTGGACATATCTTGGTCATGCACATATGCAGCTAAACAATGAGAAAGATTCTCGAGATATGGAAGCTTGTGGGTGGGATGATGATTTCTTGGAGGAGATTGGATTGGGTGATCTTGTAGATGGTCATCATGCTAAGATAGGACGAAGTGTAGCCTTCCCTGGCCATCCTCTGGGTTCTGGTCTCACACCAACTGCTGCGAAGGAATTAGATCTTGTAGCAGGAATCCCTGTTGGAACTTCCCTAATTGATGCTCATGCCGGTGGTGTGGGGGTAATGGAAAGTGTGCCTGTGACATATTCTGAGCCTAAAGAGTGTGATGGTGAAGCTCTCTGCCATCGTATGGTGTTAGTCTGTGGAACTTCTACATGCCATATGGTTGTATCTCGGGACAAACTTTTCATTCCAGGGGTCTGGGGCCCATACTGGTCAGCTATGGTGCCCGAATATTGGCTCACAGAAGGTGGACAGAGTGCTACAGGTGCATTATTGGATCACATAATTGAAAGCCATCTTGCGGCTCCTTACCTTGCAAATCATGCTGCTTCTCAGTGCATTTCTTTGTTTGAACTACTGAAGAAGTTACTGAACATAATGATGCATGATCTGAAGCGTCCATTTGTTGCTTCTTTGACTGAAGACATGCATGTTCTTCCAGATTTTCATGGGAACAGGTCTCCCAATGCAGACCCAAAGGCAAAAGGTATGGTATGTGGCTTAACACTTGATACAAGTCAGAATCAGTTGGCTCTTCAGTATCTTGCCACAGTACAAGGTATTGCATATGGCACACGTCACATTGTAGAGCACTGCAACGCCAATGGTCACAAAATTGACACAATACTAGCATGTGGAGGCCTTGCTAAGAATGCACTATTCATTCAAGAACATGCAGATATAATAGGTTGCCCTATAATTCTTCCACGAGAAAGTGAACCTGTGCTCTTGGGTGCTGCCATTCTTGGTGCTGTTGCTGCAAGGAAATATTCTAGTCTGCATGAGGCCATGAAGGCTCTGAATGCAGCGGGCCAGGTCATCCATCCATCCAAAGATCCTAGGGTGAAGAAGTACCATGATGCCAAGTACCGCATTTTTCGCGAGCTTTATGAGCAGCAGCTATCTCATCGTTCTCTGATGGCTCAAGCCTTGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FGGY_C FGGY_N

Protein Analysis

631

Amino Acids

68.39

Weight (kDa)

6.15

Isoelectric Point (pI)

49.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGGY_N	PF00370	66 - 336	6.9e-23	FGGY family of carbohydrate kinases, N-terminal domain
FGGY_C	PF02782	362 - 572	4.4e-55	FGGY family of carbohydrate kinases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002316)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30310 AT4G30310 AT4G30310 AT4G30310
fragaria_vesca	FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080
malus_domestica	MD13G1267100.v1.1
prunus_persica	Prupe.1G089400_v2.0.a1 Prupe.1G089400_v2.0.a1
pyrus_communis	pycom13g22860
rosa_chinensis	RchiOBHm_Chr4g0403081 RchiOBHm_Chr4g0405011 RchiOBHm_Chr4g0405021
rosa_laevigata	RLG00000009027
rosa_multiflora	Rmu_co8073462.1_g000001 Rmu_co8189736.1_g000001 Rmu_sc0000225.1_g000012 Rmu_sc0001023.1_g000030 Rmu_ssc0000127.1_g000034
rosa_roxburghii	Rroxscaffold_5G00347760
rosa_rugosa	Rorug04G0040500 Rorug04G0052000 Rorug04G0052100
rosa_samantha	Rh4AG117900 Rh4AG127000 Rh4AG127200 Rh4BG110300 Rh4BG121100 Rh4CG124700 Rh4CG135100 Rh4CG135200 Rh4DG109800 Rh4DG110300 Rh4DG121400
rosa_wichuraiana	Rw4G009430 Rw4G009520 Rw4G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	224
AccB1I	GGYRCC	2 cut(s)	215, 1177
AccB7I	CCANNNNNTGG	2 cut(s)	910, 1081
AccBSI	CCGCTC	1 cut(s)	189
AccII	CGCG	2 cut(s)	231, 1836
AclWI	GGATC	2 cut(s)	1239, 1784
AcoI	YGGCCR	1 cut(s)	901
AcsI	RAATTY	1 cut(s)	127
AcuI	CTGAAG	5 cut(s)	643, 1334, 1367, 1395, 1475
AfaI	GTAC	5 cut(s)	572, 727, 1509, 1808, 1823
AfiI	CCNNNNNNNGG	4 cut(s)	910, 1081, 1188, 1255
AflIII	ACRYGT	1 cut(s)	1529
AgeI	ACCGGT	1 cut(s)	218
AgsI	TTSAA	6 cut(s)	126, 486, 527, 1244, 1307, 1622
AjiI	CACGTC	1 cut(s)	1532
AjnI	CCWGG	3 cut(s)	898, 1144, 1767
Alw21I	GWGCWC	4 cut(s)	371, 586, 1548, 1683
Alw26I	GTCTC	4 cut(s)	148, 725, 926, 1419
AlwI	GGATC	2 cut(s)	1239, 1784
Ama87I	CYCGRG	3 cut(s)	149, 792, 1125
AoxI	GGCC	6 cut(s)	696, 901, 1156, 1594, 1740, 1765
ApaI	GGGCCC	1 cut(s)	1160
ApoI	RAATTY	1 cut(s)	127
AseI	ATTAAT	1 cut(s)	543
AsiGI	ACCGGT	1 cut(s)	218
Asp700I	GAANNNNTTC	1 cut(s)	633
AspA2I	CCTAGG	1 cut(s)	1793
AspLEI	GCGC	2 cut(s)	227, 233
AspS9I	GGNCC	6 cut(s)	511, 670, 696, 1156, 1157, 1765
AsuHPI	GGTGA	5 cut(s)	491, 634, 860, 1073, 1810
AvaI	CYCGRG	3 cut(s)	149, 792, 1125
AvaII	GGWCC	2 cut(s)	511, 670
AvrII	CCTAGG	1 cut(s)	1793
BaeGI	GKGCMC	2 cut(s)	1160, 1182
BalI	TGGCCA	1 cut(s)	903
BanI	GGYRCC	2 cut(s)	215, 1177
BanII	GRGCYC	2 cut(s)	586, 1160
BauI	CACGAG	1 cut(s)	1662
BbsI	GAAGAC	3 cut(s)	187, 649, 1383
Bbv12I	GWGCWC	4 cut(s)	371, 586, 1548, 1683
BceAI	ACGGC	2 cut(s)	152, 466
BciT130I	CCWGG	3 cut(s)	900, 1146, 1769
BcoDI	GTCTC	4 cut(s)	148, 725, 926, 1419
BfaI	CTAG	4 cut(s)	263, 1583, 1728, 1794
BfmI	CTRYAG	1 cut(s)	1215
BfoI	RGCGCY	1 cut(s)	228
BfuAI	ACCTGC	1 cut(s)	224
BglI	GCCNNNNNGGC	1 cut(s)	1748
BglII	AGATCT	1 cut(s)	949
BlnI	CCTAGG	1 cut(s)	1793
BmcAI	AGTACT	1 cut(s)	572
Bme1390I	CCNGG	3 cut(s)	900, 1146, 1769
Bme18I	GGWCC	2 cut(s)	511, 670
BmeT110I	CYCGRG	3 cut(s)	149, 792, 1125
BmgBI	CACGTC	1 cut(s)	1532
BmgT120I	GGNCC	6 cut(s)	511, 670, 696, 1156, 1157, 1765
BmiI	GGNNCC	5 cut(s)	217, 1157, 1158, 1179, 1260
BmrFI	CCNGG	3 cut(s)	900, 1146, 1769
BmsI	GCATC	5 cut(s)	433, 588, 979, 1326, 1804
BpiI	GAAGAC	3 cut(s)	187, 649, 1383
BplI	GAGNNNNNCTC	2 cut(s)	143, 175
BpuEI	CTTGAG	1 cut(s)	1863
BsaAI	YACGTR	1 cut(s)	551
BsaBI	GATNNNNATC	1 cut(s)	864
BsaI	GGTCTC	2 cut(s)	926, 1419
BsaJI	CCNNGG	6 cut(s)	199, 387, 898, 1145, 1793, 1884
BsaWI	WCCGGW	1 cut(s)	218
BsaXI	ACNNNNNCTCC	2 cut(s)	709, 739
Bsc4I	CCNNNNNNNGG	4 cut(s)	910, 1081, 1188, 1255
Bse118I	RCCGGY	2 cut(s)	218, 998
Bse1I	ACTGG	1 cut(s)	1169
Bse8I	GATNNNNATC	1 cut(s)	864
BseBI	CCWGG	3 cut(s)	900, 1146, 1769
BseDI	CCNNGG	6 cut(s)	199, 387, 898, 1145, 1793, 1884
BseGI	GGATG	8 cut(s)	448, 513, 672, 823, 904, 1773, 1777, 1781
BseJI	GATNNNNATC	1 cut(s)	864
BseLI	CCNNNNNNNGG	4 cut(s)	910, 1081, 1188, 1255
BseMII	CTCAG	6 cut(s)	153, 585, 630, 665, 1032, 1301
BseNI	ACTGG	1 cut(s)	1169
BseRI	GAGGAG	4 cut(s)	109, 127, 151, 848
BseSI	GKGCMC	2 cut(s)	1160, 1182
Bsh1236I	CGCG	2 cut(s)	231, 1836
BshFI	GGCC	6 cut(s)	698, 903, 1158, 1596, 1742, 1767
BshNI	GGYRCC	2 cut(s)	215, 1177
BshTI	ACCGGT	1 cut(s)	218
BsiHKAI	GWGCWC	4 cut(s)	371, 586, 1548, 1683
BsiHKCI	CYCGRG	3 cut(s)	149, 792, 1125
BsiSI	CCGG	2 cut(s)	219, 999
BslFI	GGGAC	2 cut(s)	155, 1142
BslI	CCNNNNNNNGG	4 cut(s)	910, 1081, 1188, 1255
BsmAI	GTCTC	4 cut(s)	148, 725, 926, 1419
BsmFI	GGGAC	2 cut(s)	155, 1142
BsmI	GAATGC	2 cut(s)	1612, 1762
BsnI	GGCC	6 cut(s)	698, 903, 1158, 1596, 1742, 1767
Bso31I	GGTCTC	2 cut(s)	926, 1419
BsoBI	CYCGRG	3 cut(s)	149, 792, 1125
Bsp120I	GGGCCC	1 cut(s)	1156
Bsp1286I	GDGCHC	6 cut(s)	371, 586, 1160, 1182, 1548, 1683
Bsp143I	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
Bsp68I	TCGCGA	1 cut(s)	1836
BspANI	GGCC	6 cut(s)	698, 903, 1158, 1596, 1742, 1767
BspCNI	CTCAG	6 cut(s)	152, 586, 631, 666, 1033, 1300
BspFNI	CGCG	2 cut(s)	231, 1836
BspLI	GGNNCC	5 cut(s)	217, 1157, 1158, 1179, 1260
BspMI	ACCTGC	1 cut(s)	224
BspPI	GGATC	2 cut(s)	1239, 1784
BspQI	GCTCTTC	1 cut(s)	1494
BspT107I	GGYRCC	2 cut(s)	215, 1177
BspTNI	GGTCTC	2 cut(s)	926, 1419
BsrBI	CCGCTC	1 cut(s)	189
BsrFI	RCCGGY	2 cut(s)	218, 998
BsrI	ACTGG	1 cut(s)	1169
BssAI	RCCGGY	2 cut(s)	218, 998
BssECI	CCNNGG	6 cut(s)	199, 387, 898, 1145, 1793, 1884
BssMI	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
BssSI	CACGAG	1 cut(s)	1662
BssT1I	CCWWGG	3 cut(s)	387, 1793, 1884
Bst2BI	CACGAG	1 cut(s)	1662
Bst2UI	CCWGG	3 cut(s)	900, 1146, 1769
Bst4CI	ACNGT	5 cut(s)	466, 562, 575, 733, 1507
Bst6I	CTCTTC	2 cut(s)	127, 1494
BstAPI	GCANNNNNTGC	3 cut(s)	347, 443, 1277
BstBAI	YACGTR	1 cut(s)	551
BstC8I	GCNNGC	6 cut(s)	187, 229, 339, 367, 1765, 1838
BstDEI	CTNAG	8 cut(s)	139, 594, 639, 674, 873, 1041, 1287, 1602
BstF5I	GGATG	8 cut(s)	448, 513, 672, 823, 904, 1773, 1777, 1781
BstFNI	CGCG	2 cut(s)	231, 1836
BstH2I	RGCGCY	1 cut(s)	228
BstHHI	GCGC	2 cut(s)	227, 233
BstKTI	GATC	5 cut(s)	853, 952, 1234, 1345, 1792
BstMAI	GTCTC	4 cut(s)	148, 725, 926, 1419
BstMBI	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
BstNI	CCWGG	3 cut(s)	900, 1146, 1769
BstNSI	RCATGY	6 cut(s)	341, 1110, 1384, 1388, 1590, 1631
BstSCI	CCNGG	3 cut(s)	898, 1144, 1767
BstSFI	CTRYAG	1 cut(s)	1215
BstSLI	GKGCMC	2 cut(s)	1160, 1182
BstUI	CGCG	2 cut(s)	231, 1836
BstV2I	GAAGAC	3 cut(s)	187, 649, 1383
BstX2I	RGATCY	2 cut(s)	949, 1789
BstXI	CCANNNNNNTGG	1 cut(s)	1152
BstYI	RGATCY	2 cut(s)	949, 1789
BsuRI	GGCC	6 cut(s)	698, 903, 1158, 1596, 1742, 1767
BtrI	CACGTC	1 cut(s)	1532
BtsCI	GGATG	8 cut(s)	448, 513, 672, 823, 904, 1773, 1777, 1781
BtsI	GCAGTG	2 cut(s)	444, 1546
BtsIMutI	CAGTG	4 cut(s)	444, 738, 1295, 1546
BtuMI	TCGCGA	1 cut(s)	1836
BveI	ACCTGC	1 cut(s)	224
Cac8I	GCNNGC	6 cut(s)	187, 229, 339, 367, 1765, 1838
CfoI	GCGC	2 cut(s)	227, 233
Cfr10I	RCCGGY	2 cut(s)	218, 998
Cfr13I	GGNCC	6 cut(s)	511, 670, 696, 1156, 1157, 1765
CseI	GACGC	1 cut(s)	1340
Csp6I	GTAC	5 cut(s)	571, 726, 1508, 1807, 1822
CspAI	ACCGGT	1 cut(s)	218
CviQI	GTAC	5 cut(s)	571, 726, 1508, 1807, 1822
DdeI	CTNAG	8 cut(s)	139, 594, 639, 674, 873, 1041, 1287, 1602
DpnI	GATC	5 cut(s)	852, 951, 1233, 1344, 1791
DpnII	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
EaeI	YGGCCR	1 cut(s)	901
Eam1104I	CTCTTC	2 cut(s)	127, 1494
EarI	CTCTTC	2 cut(s)	127, 1494
EciI	GGCGGA	1 cut(s)	154
Ecl136II	GAGCTC	1 cut(s)	584
Eco130I	CCWWGG	3 cut(s)	387, 1793, 1884
Eco147I	AGGCCT	1 cut(s)	1596
Eco24I	GRGCYC	2 cut(s)	586, 1160
Eco31I	GGTCTC	2 cut(s)	926, 1419
Eco47I	GGWCC	2 cut(s)	511, 670
Eco53kI	GAGCTC	1 cut(s)	584
Eco57I	CTGAAG	5 cut(s)	643, 1334, 1367, 1395, 1475
Eco88I	CYCGRG	3 cut(s)	149, 792, 1125
EcoICRI	GAGCTC	1 cut(s)	584
EcoO109I	RGGNCCY	1 cut(s)	1156
EcoRII	CCWGG	3 cut(s)	898, 1144, 1767
EcoT14I	CCWWGG	3 cut(s)	387, 1793, 1884
EcoT22I	ATGCAT	2 cut(s)	1341, 1386
EcoT38I	GRGCYC	2 cut(s)	586, 1160
ErhI	CCWWGG	3 cut(s)	387, 1793, 1884
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	2 cut(s)	155, 1142
FauI	CCCGC	3 cut(s)	194, 236, 1756
FauNDI	CATATG	3 cut(s)	765, 1113, 1522
FokI	GGATG	8 cut(s)	455, 520, 679, 830, 891, 1760, 1764, 1768
FriOI	GRGCYC	2 cut(s)	586, 1160
FspBI	CTAG	4 cut(s)	263, 1583, 1728, 1794
GlaI	GCGC	2 cut(s)	226, 232
HaeII	RGCGCY	1 cut(s)	228
HaeIII	GGCC	6 cut(s)	698, 903, 1158, 1596, 1742, 1767
HapII	CCGG	2 cut(s)	219, 999
HgaI	GACGC	1 cut(s)	1340
HhaI	GCGC	2 cut(s)	227, 233
Hin6I	GCGC	2 cut(s)	225, 231
HinP1I	GCGC	2 cut(s)	225, 231
HindIII	AAGCTT	2 cut(s)	611, 804
HinfI	GANTC	9 cut(s)	29, 283, 305, 482, 641, 718, 788, 963, 1477
HpaII	CCGG	2 cut(s)	219, 999
HphI	GGTGA	5 cut(s)	491, 634, 860, 1073, 1810
Hpy166II	GTNNAC	5 cut(s)	324, 428, 744, 1205, 1673
Hpy188I	TCNGA	8 cut(s)	28, 288, 717, 1042, 1347, 1476, 1755, 1872
Hpy8I	GTNNAC	5 cut(s)	324, 428, 744, 1205, 1673
Hpy99I	CGWCG	1 cut(s)	248
HpyAV	CCTTC	6 cut(s)	9, 394, 904, 934, 1193, 1741
HpyCH4III	ACNGT	5 cut(s)	466, 562, 575, 733, 1507
HpyCH4IV	ACGT	2 cut(s)	550, 1531
HpyF3I	CTNAG	8 cut(s)	139, 594, 639, 674, 873, 1041, 1287, 1602
HpySE526I	ACGT	2 cut(s)	550, 1531
HspAI	GCGC	2 cut(s)	225, 231
Kzo9I	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	5 cut(s)	122, 194, 374, 581, 1264
LweI	GCATC	5 cut(s)	433, 588, 979, 1326, 1804
MaeI	CTAG	4 cut(s)	263, 1583, 1728, 1794
MaeII	ACGT	2 cut(s)	550, 1531
MaeIII	GTNAC	6 cut(s)	460, 551, 1030, 1320, 1532, 1562
MalI	GATC	5 cut(s)	852, 951, 1233, 1344, 1791
MbiI	CCGCTC	1 cut(s)	189
MboI	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
MflI	RGATCY	2 cut(s)	949, 1789
MhlI	GDGCHC	6 cut(s)	371, 586, 1160, 1182, 1548, 1683
MlsI	TGGCCA	1 cut(s)	903
MluCI	AATT	7 cut(s)	127, 544, 944, 984, 1239, 1569, 1653
MluNI	TGGCCA	1 cut(s)	903
MlyI	GAGTC	2 cut(s)	650, 727
MmeI	TCCRAC	1 cut(s)	952
Mox20I	TGGCCA	1 cut(s)	903
Mph1103I	ATGCAT	2 cut(s)	1341, 1386
MroXI	GAANNNNTTC	1 cut(s)	633
MscI	TGGCCA	1 cut(s)	903
MseI	TTAA	3 cut(s)	543, 1457, 1891
MslI	CAYNNNNRTG	2 cut(s)	999, 1334
Msp20I	TGGCCA	1 cut(s)	903
MspA1I	CMGCKG	1 cut(s)	1763
MspI	CCGG	2 cut(s)	219, 999
MspR9I	CCNGG	3 cut(s)	900, 1146, 1769
Mva1269I	GAATGC	2 cut(s)	1612, 1762
MvaI	CCWGG	3 cut(s)	900, 1146, 1769
MvnI	CGCG	2 cut(s)	231, 1836
NdeI	CATATG	3 cut(s)	765, 1113, 1522
NdeII	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
NlaIV	GGNNCC	5 cut(s)	217, 1157, 1158, 1179, 1260
NmeAIII	GCCGAG	1 cut(s)	180
NmuCI	GTSAC	3 cut(s)	1030, 1532, 1562
NruI	TCGCGA	1 cut(s)	1836
NsiI	ATGCAT	2 cut(s)	1341, 1386
NspI	RCATGY	6 cut(s)	341, 1110, 1384, 1388, 1590, 1631
PaeI	GCATGC	1 cut(s)	341
PaeR7I	CTCGAG	2 cut(s)	149, 792
PceI	AGGCCT	1 cut(s)	1596
PciSI	GCTCTTC	1 cut(s)	1494
PcsI	WCGNNNNNNNCGW	1 cut(s)	278
PctI	GAATGC	2 cut(s)	1612, 1762
PdmI	GAANNNNTTC	1 cut(s)	633
PfeI	GAWTC	7 cut(s)	29, 283, 305, 482, 788, 963, 1477
PflMI	CCANNNNNTGG	2 cut(s)	910, 1081
PinAI	ACCGGT	1 cut(s)	218
PleI	GAGTC	2 cut(s)	649, 726
PpsI	GAGTC	2 cut(s)	649, 726
Ppu21I	YACGTR	1 cut(s)	551
PshBI	ATTAAT	1 cut(s)	543
Psp124BI	GAGCTC	1 cut(s)	586
Psp6I	CCWGG	3 cut(s)	898, 1144, 1767
PspGI	CCWGG	3 cut(s)	898, 1144, 1767
PspN4I	GGNNCC	5 cut(s)	217, 1157, 1158, 1179, 1260
PspOMI	GGGCCC	1 cut(s)	1156
PspPI	GGNCC	6 cut(s)	511, 670, 696, 1156, 1157, 1765
PsuI	RGATCY	2 cut(s)	949, 1789
RruI	TCGCGA	1 cut(s)	1836
RsaI	GTAC	5 cut(s)	572, 727, 1509, 1808, 1823
RsaNI	GTAC	5 cut(s)	571, 726, 1508, 1807, 1822
RseI	CAYNNNNRTG	2 cut(s)	999, 1334
SacI	GAGCTC	1 cut(s)	586
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	3 cut(s)	543, 1457, 1891
Sau3AI	GATC	5 cut(s)	850, 949, 1231, 1342, 1789
Sau96I	GGNCC	6 cut(s)	511, 670, 696, 1156, 1157, 1765
ScaI	AGTACT	1 cut(s)	572
SchI	GAGTC	2 cut(s)	650, 727
ScrFI	CCNGG	3 cut(s)	900, 1146, 1769
SduI	GDGCHC	6 cut(s)	371, 586, 1160, 1182, 1548, 1683
SfaNI	GCATC	5 cut(s)	433, 588, 979, 1326, 1804
SfcI	CTRYAG	1 cut(s)	1215
Sfr274I	CTCGAG	2 cut(s)	149, 792
SinI	GGWCC	2 cut(s)	511, 670
SlaI	CTCGAG	2 cut(s)	149, 792
SmiMI	CAYNNNNRTG	2 cut(s)	999, 1334
SmlI	CTYRAG	3 cut(s)	149, 792, 1878
SmoI	CTYRAG	3 cut(s)	149, 792, 1878
SphI	GCATGC	1 cut(s)	341
Sse9I	AATT	7 cut(s)	127, 544, 944, 984, 1239, 1569, 1653
SseBI	AGGCCT	1 cut(s)	1596
SspI	AATATT	2 cut(s)	1187, 1724
SspMI	CTAG	4 cut(s)	263, 1583, 1728, 1794
SstI	GAGCTC	1 cut(s)	586
StuI	AGGCCT	1 cut(s)	1596
StyD4I	CCNGG	3 cut(s)	898, 1144, 1767
StyI	CCWWGG	3 cut(s)	387, 1793, 1884
TaaI	ACNGT	5 cut(s)	466, 562, 575, 733, 1507
TaiI	ACGT	2 cut(s)	553, 1534
TaqI	TCGA	5 cut(s)	150, 243, 272, 281, 793
TasI	AATT	7 cut(s)	127, 544, 944, 984, 1239, 1569, 1653
TatI	WGTACW	2 cut(s)	570, 1507
TauI	GCSGC	3 cut(s)	353, 419, 1259
TfiI	GAWTC	7 cut(s)	29, 283, 305, 482, 788, 963, 1477
Tru1I	TTAA	3 cut(s)	543, 1457, 1891
Tru9I	TTAA	3 cut(s)	543, 1457, 1891
TscAI	CASTG	4 cut(s)	444, 738, 1295, 1553
TseFI	GTSAC	3 cut(s)	1030, 1532, 1562
Tsp45I	GTSAC	3 cut(s)	1030, 1532, 1562
TspDTI	ATGAA	4 cut(s)	1129, 1391, 1606, 1760
TspGWI	ACGGA	2 cut(s)	89, 181
TspRI	CASTG	4 cut(s)	444, 738, 1295, 1553
Van91I	CCANNNNNTGG	2 cut(s)	910, 1081
VpaK11BI	GGWCC	2 cut(s)	511, 670
VspI	ATTAAT	1 cut(s)	543
XapI	RAATTY	1 cut(s)	127
XceI	RCATGY	6 cut(s)	341, 1110, 1384, 1388, 1590, 1631
XcmI	CCANNNNNNNNNTGG	1 cut(s)	615
XhoI	CTCGAG	2 cut(s)	149, 792
XmaJI	CCTAGG	1 cut(s)	1793
XmnI	GAANNNNTTC	1 cut(s)	633
XspI	CTAG	4 cut(s)	263, 1583, 1728, 1794
ZrmI	AGTACT	1 cut(s)	572
Zsp2I	ATGCAT	2 cut(s)	1341, 1386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.