Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002316

Physical Location & Seq

Genomic Coordinates Forward (+)

25354027 .. 25362065

Gene Structure 8039 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG117900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGATCAAAGTCCAAATCCTTGAAAGAGAAGAAGAAAACAAAGAAAGCGAAATGTCTGGTAAATGGACTCGCTCCGATTCAACACCACTCCTTCGCCCCCAACACCATCCATCACAACGCTCCTCCCCCCCAAACGCTTATTCCTCATACTCTTCCATCTCTCTCGAAATGACCTCCGCCGTCTCCGCCACCCCCACCGCCACCGCCCGCTCCGTCTTCCTCGGCGTCGACGTCGGCACCGGCAGCGCCCGCGCAGGTGTGTTTGATGAGAATGGGAAGCTTCTAGGTTCGTCTAGCAGTCCCATTCAGATTTGGAAGGACGGTGATTGCGTTGAGCAATCTTCGACGGATATCTGGCATGCAATAAGTGCGGCTGTAAAAGCAGCATGCTCCCTTGCAGAAGTTTCCGGGGATGAAGTGAAGGGTCTGGGATTCGCGGCTACTTGTTCACTAGTTGCAGTGGATGCCGATGGCTCTCCTGTTACAGTTTCCTGGAGTGGTGATTCAAGGAGAAACATAATAGTATGGATGGACCATAGAGCTGTAGAACAAGCTGAAAGGATCAATTCACGCAACTCACCCGTCCTGCAGTACTGTGGTGGAGCTCTTTCCCCTGAGATGCAGCCTCCAAAGCTTTTATGGGTGAAAGAAAATCTGCCAGAATCTTGGTCAATGGTCTTCAGGTGGATGGACTTGAGTGATTGGTTATCATACAGGGCCACAGGAGATGATACGCGTAGTCTCTGTACAACAGTTTGTAAATGGACATATCTTGGTCATGCACATATGCAGCAAAACAATGAGAAAGATTCTCGAGACATGGAAGCTTGTGGATGGGATGATGATTTCTGGGAGGAGATTGGATTAGGTGATCTTGTAGAAGGGCATCATGCTAAGATAGGACGAAGTGTAGCCTTCCCTGGCCATCCTCTGGGTTCTGGTCTCACATCTACTGCTGCGAAGGAACTAGGTCTTGTACCAGGGATTCCTGTTGGGACTTCGCTAATTGATGCTCATGCCGGTGGTGTGGGGGTATTGGAAGGTGTGCCTTCATCAGATTGTCACGCTAAAGAGTCTGATATTGAAGCTCTATGCCATCGTATGGTGTTAGTCTGCGGAACTTCTACTTGCCATATGGTTGTATCACAGAAGAAGCTTTTCATTCCAGGTGTCTGGGGTCCGTATTGGTCAGCTATGGTACCTGAACAGTGGCTTACAGAAGGTGGTCAGAGTGCTACTGGTGCATTATTGGATTACATAATTGAAAACCATGTTGCTTCTCCTCACCTTGCCAATCGTGCTGCTTCTCAACGCATTTCTGTGTTTGAGCTTCTGAACAAGATATTGGAAACAATGATGCATGATCTGAAGCGTCCGTTCCTTGCTTCTTTGACCGAAGATATTCATGTTCTTCCCGATTTTCATGGGAACAGGTCGCCAATTGCCGATCCAAAAGCAAAAGGAATTATCTGTGGTTTAACACTTGATACAAGTGAGAAGCAATTGGCACTTCAGTACCTCGCCACACTGCAAGGTATTGCATATGGCACTCGCCATATTGTAGAGCACTGCAATGCCCAGGGTCACAAAATTGACACTATACTAGCATGTGGAGGCCTTGTTAAGAACCCACTATTCATACAAGAACACTCGGATATAATCGGTTGCCCTATTATTCTTCCACGGGAGAGTGAGCCTGTGCTATTGGGTGCTGCCATTCTTGGTGCTGTAGCTGCAAGGAAATATTCTAGTATGCATGATGCCATGAAGGCCCTGAACGCGTCGGGGCAGGTATGTTTTTTCGAACGTTGCTCTAGTACTTCTAATTGTTTTCTTTACTCTATTGGCACGCAAATTGGAACCTCTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FGGY_C FGGY_N

Protein Analysis

623

Amino Acids

67.43

Weight (kDa)

5.58

Isoelectric Point (pI)

50.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGGY_N	PF00370	73 - 344	4.1e-23	FGGY family of carbohydrate kinases, N-terminal domain
FGGY_C	PF02782	369 - 579	2.5e-56	FGGY family of carbohydrate kinases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002316)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30310 AT4G30310 AT4G30310 AT4G30310
fragaria_vesca	FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080 FvH4_4g09080
malus_domestica	MD13G1267100.v1.1
prunus_persica	Prupe.1G089400_v2.0.a1 Prupe.1G089400_v2.0.a1
pyrus_communis	pycom13g22860
rosa_chinensis	RchiOBHm_Chr4g0403081 RchiOBHm_Chr4g0405011 RchiOBHm_Chr4g0405021
rosa_laevigata	RLG00000009027
rosa_multiflora	Rmu_co8073462.1_g000001 Rmu_co8189736.1_g000001 Rmu_sc0000225.1_g000012 Rmu_sc0001023.1_g000030 Rmu_ssc0000127.1_g000034
rosa_roxburghii	Rroxscaffold_5G00347760
rosa_rugosa	Rorug04G0040500 Rorug04G0052000 Rorug04G0052100
rosa_samantha	Rh4AG117900 Rh4AG127000 Rh4AG127200 Rh4BG110300 Rh4BG121100 Rh4CG124700 Rh4CG135100 Rh4CG135200 Rh4DG109800 Rh4DG110300 Rh4DG121400
rosa_wichuraiana	Rw4G009430 Rw4G009520 Rw4G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	245
AatII	GACGTC	1 cut(s)	234
Acc36I	ACCTGC	2 cut(s)	245, 1780
Acc65I	GGTACC	1 cut(s)	1198
AccB1I	GGYRCC	2 cut(s)	236, 1198
AccB7I	CCANNNNNTGG	2 cut(s)	931, 1102
AccBSI	CCGCTC	1 cut(s)	210
AccI	GTMKAC	1 cut(s)	228
AccII	CGCG	4 cut(s)	252, 437, 736, 1781
AciI	CCGC	9 cut(s)	177, 186, 198, 204, 208, 250, 371, 437, 1116
AclI	AACGTT	1 cut(s)	1807
AclWI	GGATC	2 cut(s)	569, 1442
AcoI	YGGCCR	1 cut(s)	922
AcuI	CTGAAG	3 cut(s)	664, 1388, 1496
AcyI	GRCGYC	2 cut(s)	225, 231
AfaI	GTAC	6 cut(s)	593, 748, 978, 1200, 1517, 1819
AfiI	CCNNNNNNNGG	2 cut(s)	931, 1102
AflIII	ACRYGT	2 cut(s)	734, 1779
AgsI	TTSAA	5 cut(s)	23, 81, 507, 1085, 1265
AhlI	ACTAGT	1 cut(s)	451
AjnI	CCWGG	5 cut(s)	491, 919, 979, 1165, 1578
AjuI	GAANNNNNNNTTGG	4 cut(s)	124, 156, 1328, 1360
Alw21I	GWGCWC	2 cut(s)	607, 1569
Alw26I	GTCTC	4 cut(s)	187, 746, 810, 947
AlwI	GGATC	2 cut(s)	569, 1442
Ama87I	CYCGRG	1 cut(s)	813
AoxI	GGCC	4 cut(s)	717, 922, 1615, 1770
ApeKI	GCWGC	8 cut(s)	243, 383, 622, 790, 956, 1301, 1712, 1733
Asp700I	GAANNNNTTC	2 cut(s)	1157, 1401
Asp718I	GGTACC	1 cut(s)	1198
AspLEI	GCGC	2 cut(s)	248, 254
AspS9I	GGNCC	4 cut(s)	532, 717, 1178, 1771
AsuC2I	CCSGG	1 cut(s)	409
AsuHPI	GGTGA	6 cut(s)	335, 512, 570, 655, 881, 1277
AsuII	TTCGAA	1 cut(s)	1803
AvaI	CYCGRG	1 cut(s)	813
AvaII	GGWCC	2 cut(s)	532, 1178
BaeI	ACNNNNGTAYC	2 cut(s)	1499, 1532
BalI	TGGCCA	1 cut(s)	924
BanI	GGYRCC	2 cut(s)	236, 1198
BanII	GRGCYC	1 cut(s)	607
BbsI	GAAGAC	2 cut(s)	208, 670
Bbv12I	GWGCWC	2 cut(s)	607, 1569
BbvI	GCAGC	8 cut(s)	255, 395, 634, 802, 943, 1288, 1699, 1720
BccI	CCATC	9 cut(s)	114, 118, 164, 464, 523, 682, 828, 933, 1104
BceAI	ACGGC	1 cut(s)	164
BciT130I	CCWGG	5 cut(s)	493, 921, 981, 1167, 1580
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	1 cut(s)	409
BcoDI	GTCTC	4 cut(s)	187, 746, 810, 947
BcuI	ACTAGT	1 cut(s)	451
BfaI	CTAG	8 cut(s)	284, 294, 452, 968, 1604, 1749, 1815, 1870
BfmI	CTRYAG	3 cut(s)	543, 587, 1728
BfoI	RGCGCY	1 cut(s)	249
BfuAI	ACCTGC	2 cut(s)	245, 1780
BglI	GCCNNNNNGGC	1 cut(s)	1769
BmcAI	AGTACT	2 cut(s)	593, 1819
Bme1390I	CCNGG	6 cut(s)	409, 493, 921, 981, 1167, 1580
Bme18I	GGWCC	2 cut(s)	532, 1178
BmeT110I	CYCGRG	1 cut(s)	813
BmgT120I	GGNCC	4 cut(s)	532, 717, 1178, 1771
BmiI	GGNNCC	4 cut(s)	238, 1179, 1200, 1861
BmrFI	CCNGG	6 cut(s)	409, 493, 921, 981, 1167, 1580
BmsI	GCATC	6 cut(s)	454, 609, 895, 1000, 1347, 1750
BpiI	GAAGAC	2 cut(s)	208, 670
BpmI	CTGGAG	1 cut(s)	514
Bpu14I	TTCGAA	1 cut(s)	1803
BpuEI	CTTGAG	1 cut(s)	715
BpuMI	CCSGG	1 cut(s)	409
BsaHI	GRCGYC	2 cut(s)	225, 231
BsaI	GGTCTC	1 cut(s)	947
BsaJI	CCNNGG	7 cut(s)	220, 408, 919, 980, 1578, 1579, 1682
BsaXI	ACNNNNNCTCC	2 cut(s)	1605, 1635
Bsc4I	CCNNNNNNNGG	2 cut(s)	931, 1102
Bse118I	RCCGGY	2 cut(s)	239, 1019
Bse1I	ACTGG	1 cut(s)	1243
Bse3DI	GCAATG	1 cut(s)	1579
BseBI	CCWGG	5 cut(s)	493, 921, 981, 1167, 1580
BseDI	CCNNGG	7 cut(s)	220, 408, 919, 980, 1578, 1579, 1682
BseGI	GGATG	8 cut(s)	106, 418, 469, 534, 693, 839, 844, 925
BseLI	CCNNNNNNNGG	2 cut(s)	931, 1102
BseMI	GCAATG	1 cut(s)	1579
BseMII	CTCAG	1 cut(s)	606
BseNI	ACTGG	1 cut(s)	1243
BseRI	GAGGAG	3 cut(s)	112, 869, 1272
BseXI	GCAGC	8 cut(s)	255, 395, 634, 802, 943, 1288, 1699, 1720
Bsh1236I	CGCG	4 cut(s)	252, 437, 736, 1781
BshFI	GGCC	4 cut(s)	719, 924, 1617, 1772
BshNI	GGYRCC	2 cut(s)	236, 1198
BsiHKAI	GWGCWC	2 cut(s)	607, 1569
BsiHKCI	CYCGRG	1 cut(s)	813
BsiSI	CCGG	3 cut(s)	240, 408, 1020
BslFI	GGGAC	2 cut(s)	285, 1009
BslI	CCNNNNNNNGG	2 cut(s)	931, 1102
BsmAI	GTCTC	4 cut(s)	187, 746, 810, 947
BsmBI	CGTCTC	1 cut(s)	187
BsmFI	GGGAC	2 cut(s)	285, 1009
BsnI	GGCC	4 cut(s)	719, 924, 1617, 1772
Bso31I	GGTCTC	1 cut(s)	947
BsoBI	CYCGRG	1 cut(s)	813
Bsp119I	TTCGAA	1 cut(s)	1803
Bsp1286I	GDGCHC	2 cut(s)	607, 1569
Bsp1407I	TGTACA	1 cut(s)	746
Bsp143I	GATC	5 cut(s)	3, 561, 871, 1363, 1447
BspACI	CCGC	9 cut(s)	177, 186, 198, 204, 208, 250, 371, 437, 1116
BspANI	GGCC	4 cut(s)	719, 924, 1617, 1772
BspCNI	CTCAG	1 cut(s)	607
BspFNI	CGCG	4 cut(s)	252, 437, 736, 1781
BspLI	GGNNCC	4 cut(s)	238, 1179, 1200, 1861
BspMAI	CTGCAG	1 cut(s)	591
BspMI	ACCTGC	2 cut(s)	245, 1780
BspPI	GGATC	2 cut(s)	569, 1442
BspT104I	TTCGAA	1 cut(s)	1803
BspT107I	GGYRCC	2 cut(s)	236, 1198
BspTNI	GGTCTC	1 cut(s)	947
BsrBI	CCGCTC	1 cut(s)	210
BsrDI	GCAATG	1 cut(s)	1579
BsrFI	RCCGGY	2 cut(s)	239, 1019
BsrGI	TGTACA	1 cut(s)	746
BsrI	ACTGG	1 cut(s)	1243
BssAI	RCCGGY	2 cut(s)	239, 1019
BssECI	CCNNGG	7 cut(s)	220, 408, 919, 980, 1578, 1579, 1682
BssMI	GATC	5 cut(s)	3, 561, 871, 1363, 1447
BssNI	GRCGYC	2 cut(s)	225, 231
Bst2UI	CCWGG	5 cut(s)	493, 921, 981, 1167, 1580
Bst4CI	ACNGT	5 cut(s)	323, 487, 596, 754, 1209
Bst6I	CTCTTC	1 cut(s)	157
BstACI	GRCGYC	2 cut(s)	225, 231
BstAPI	GCANNNNNTGC	2 cut(s)	368, 464
BstAUI	TGTACA	1 cut(s)	746
BstBI	TTCGAA	1 cut(s)	1803
BstC8I	GCNNGC	5 cut(s)	208, 250, 360, 388, 1850
BstDEI	CTNAG	2 cut(s)	615, 894
BstDSI	CCRYGG	1 cut(s)	1682
BstF5I	GGATG	8 cut(s)	106, 418, 469, 534, 693, 839, 844, 925
BstFNI	CGCG	4 cut(s)	252, 437, 736, 1781
BstH2I	RGCGCY	1 cut(s)	249
BstHHI	GCGC	2 cut(s)	248, 254
BstKTI	GATC	5 cut(s)	6, 564, 874, 1366, 1450
BstMAI	GTCTC	4 cut(s)	187, 746, 810, 947
BstMBI	GATC	5 cut(s)	3, 561, 871, 1363, 1447
BstNI	CCWGG	5 cut(s)	493, 921, 981, 1167, 1580
BstNSI	RCATGY	3 cut(s)	362, 390, 1611
BstSCI	CCNGG	6 cut(s)	407, 491, 919, 979, 1165, 1578
BstSFI	CTRYAG	3 cut(s)	543, 587, 1728
BstUI	CGCG	4 cut(s)	252, 437, 736, 1781
BstV1I	GCAGC	8 cut(s)	255, 395, 634, 802, 943, 1288, 1699, 1720
BstV2I	GAAGAC	2 cut(s)	208, 670
BstXI	CCANNNNNNTGG	2 cut(s)	666, 1173
BsuRI	GGCC	4 cut(s)	719, 924, 1617, 1772
BtgI	CCRYGG	1 cut(s)	1682
BtsCI	GGATG	8 cut(s)	106, 418, 469, 534, 693, 839, 844, 925
BtsI	GCAGTG	3 cut(s)	465, 1526, 1567
BtsIMutI	CAGTG	4 cut(s)	465, 1214, 1526, 1567
BveI	ACCTGC	2 cut(s)	245, 1780
Cac8I	GCNNGC	5 cut(s)	208, 250, 360, 388, 1850
CfoI	GCGC	2 cut(s)	248, 254
Cfr10I	RCCGGY	2 cut(s)	239, 1019
Cfr13I	GGNCC	4 cut(s)	532, 717, 1178, 1771
CseI	GACGC	3 cut(s)	214, 1361, 1770
Csp6I	GTAC	6 cut(s)	592, 747, 977, 1199, 1516, 1818
CviQI	GTAC	6 cut(s)	592, 747, 977, 1199, 1516, 1818
DdeI	CTNAG	2 cut(s)	615, 894
DpnI	GATC	5 cut(s)	5, 563, 873, 1365, 1449
DpnII	GATC	5 cut(s)	3, 561, 871, 1363, 1447
EaeI	YGGCCR	1 cut(s)	922
Eam1104I	CTCTTC	1 cut(s)	157
EarI	CTCTTC	1 cut(s)	157
EciI	GGCGGA	2 cut(s)	166, 175
Ecl136II	GAGCTC	1 cut(s)	605
Eco147I	AGGCCT	1 cut(s)	1617
Eco24I	GRGCYC	1 cut(s)	607
Eco31I	GGTCTC	1 cut(s)	947
Eco32I	GATATC	1 cut(s)	352
Eco47I	GGWCC	2 cut(s)	532, 1178
Eco53kI	GAGCTC	1 cut(s)	605
Eco57I	CTGAAG	3 cut(s)	664, 1388, 1496
Eco88I	CYCGRG	1 cut(s)	813
EcoICRI	GAGCTC	1 cut(s)	605
EcoO109I	RGGNCCY	1 cut(s)	1771
EcoRII	CCWGG	5 cut(s)	491, 919, 979, 1165, 1578
EcoRV	GATATC	1 cut(s)	352
EcoT22I	ATGCAT	2 cut(s)	1362, 1758
EcoT38I	GRGCYC	1 cut(s)	607
Esp3I	CGTCTC	1 cut(s)	187
FaqI	GGGAC	2 cut(s)	285, 1009
FauI	CCCGC	2 cut(s)	215, 257
FauNDI	CATATG	3 cut(s)	786, 1134, 1543
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	1 cut(s)	228
FokI	GGATG	8 cut(s)	93, 425, 476, 541, 700, 846, 851, 912
FriOI	GRGCYC	1 cut(s)	607
FspBI	CTAG	8 cut(s)	284, 294, 452, 968, 1604, 1749, 1815, 1870
GlaI	GCGC	2 cut(s)	247, 253
GsuI	CTGGAG	1 cut(s)	514
HaeII	RGCGCY	1 cut(s)	249
HaeIII	GGCC	4 cut(s)	719, 924, 1617, 1772
HapII	CCGG	3 cut(s)	240, 408, 1020
HgaI	GACGC	3 cut(s)	214, 1361, 1770
HhaI	GCGC	2 cut(s)	248, 254
Hin1I	GRCGYC	2 cut(s)	225, 231
Hin6I	GCGC	2 cut(s)	246, 252
HinP1I	GCGC	2 cut(s)	246, 252
HincII	GTYRAC	1 cut(s)	229
HindII	GTYRAC	1 cut(s)	229
HindIII	AAGCTT	4 cut(s)	278, 632, 825, 1154
HinfI	GANTC	8 cut(s)	67, 77, 432, 503, 662, 809, 985, 1073
HpaII	CCGG	3 cut(s)	240, 408, 1020
HphI	GGTGA	6 cut(s)	335, 512, 570, 655, 881, 1277
Hpy166II	GTNNAC	2 cut(s)	229, 449
Hpy188I	TCNGA	8 cut(s)	76, 309, 1057, 1078, 1230, 1335, 1368, 1654
Hpy188III	TCNNGA	4 cut(s)	164, 813, 815, 1415
Hpy8I	GTNNAC	2 cut(s)	229, 449
Hpy99I	CGWCG	5 cut(s)	230, 233, 236, 349, 1786
HpyCH4III	ACNGT	5 cut(s)	323, 487, 596, 754, 1209
HpyCH4IV	ACGT	2 cut(s)	231, 1807
HpyF3I	CTNAG	2 cut(s)	615, 894
HpySE526I	ACGT	2 cut(s)	231, 1807
Hsp92I	GRCGYC	2 cut(s)	225, 231
HspAI	GCGC	2 cut(s)	246, 252
KpnI	GGTACC	1 cut(s)	1202
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	5 cut(s)	3, 561, 871, 1363, 1447
LmnI	GCTCC	5 cut(s)	77, 125, 215, 395, 602
Lsp1109I	GCAGC	8 cut(s)	255, 395, 634, 802, 943, 1288, 1699, 1720
LweI	GCATC	6 cut(s)	454, 609, 895, 1000, 1347, 1750
MaeI	CTAG	8 cut(s)	284, 294, 452, 968, 1604, 1749, 1815, 1870
MaeII	ACGT	2 cut(s)	231, 1807
MaeIII	GTNAC	3 cut(s)	481, 1061, 1583
MalI	GATC	5 cut(s)	5, 563, 873, 1365, 1449
MbiI	CCGCTC	1 cut(s)	210
MboI	GATC	5 cut(s)	3, 561, 871, 1363, 1447
MfeI	CAATTG	2 cut(s)	1440, 1502
MhlI	GDGCHC	2 cut(s)	607, 1569
MlsI	TGGCCA	1 cut(s)	924
MluCI	AATT	9 cut(s)	565, 1005, 1260, 1440, 1464, 1502, 1590, 1825, 1854
MluI	ACGCGT	2 cut(s)	734, 1779
MluNI	TGGCCA	1 cut(s)	924
MlyI	GAGTC	2 cut(s)	61, 1082
Mox20I	TGGCCA	1 cut(s)	924
Mph1103I	ATGCAT	2 cut(s)	1362, 1758
MroXI	GAANNNNTTC	2 cut(s)	1157, 1401
MscI	TGGCCA	1 cut(s)	924
MseI	TTAA	2 cut(s)	1478, 1623
MslI	CAYNNNNRTG	3 cut(s)	1020, 1319, 1572
Msp20I	TGGCCA	1 cut(s)	924
MspI	CCGG	3 cut(s)	240, 408, 1020
MspR9I	CCNGG	6 cut(s)	409, 493, 921, 981, 1167, 1580
MunI	CAATTG	2 cut(s)	1440, 1502
MvaI	CCWGG	5 cut(s)	493, 921, 981, 1167, 1580
MvnI	CGCG	4 cut(s)	252, 437, 736, 1781
NciI	CCSGG	1 cut(s)	409
NdeI	CATATG	3 cut(s)	786, 1134, 1543
NdeII	GATC	5 cut(s)	3, 561, 871, 1363, 1447
NlaIV	GGNNCC	4 cut(s)	238, 1179, 1200, 1861
NmeAIII	GCCGAG	1 cut(s)	201
NmuCI	GTSAC	2 cut(s)	1061, 1583
NsiI	ATGCAT	2 cut(s)	1362, 1758
NspI	RCATGY	3 cut(s)	362, 390, 1611
NspV	TTCGAA	1 cut(s)	1803
PaeI	GCATGC	2 cut(s)	362, 390
PaeR7I	CTCGAG	1 cut(s)	813
PaqCI	CACCTGC	1 cut(s)	245
PasI	CCCWGGG	1 cut(s)	1579
PceI	AGGCCT	1 cut(s)	1617
PcsI	WCGNNNNNNNCGW	2 cut(s)	228, 327
PdmI	GAANNNNTTC	2 cut(s)	1157, 1401
PfeI	GAWTC	6 cut(s)	77, 432, 503, 662, 809, 985
PflMI	CCANNNNNTGG	2 cut(s)	931, 1102
PfoI	TCCNGGA	1 cut(s)	491
PleI	GAGTC	2 cut(s)	61, 1081
PpsI	GAGTC	2 cut(s)	61, 1081
Psp124BI	GAGCTC	1 cut(s)	607
Psp1406I	AACGTT	1 cut(s)	1807
Psp6I	CCWGG	5 cut(s)	491, 919, 979, 1165, 1578
PspGI	CCWGG	5 cut(s)	491, 919, 979, 1165, 1578
PspN4I	GGNNCC	4 cut(s)	238, 1179, 1200, 1861
PspPI	GGNCC	4 cut(s)	532, 717, 1178, 1771
PstI	CTGCAG	1 cut(s)	591
RsaI	GTAC	6 cut(s)	593, 748, 978, 1200, 1517, 1819
RsaNI	GTAC	6 cut(s)	592, 747, 977, 1199, 1516, 1818
RseI	CAYNNNNRTG	3 cut(s)	1020, 1319, 1572
SacI	GAGCTC	1 cut(s)	607
SalI	GTCGAC	1 cut(s)	227
SaqAI	TTAA	2 cut(s)	1478, 1623
Sau3AI	GATC	5 cut(s)	3, 561, 871, 1363, 1447
Sau96I	GGNCC	4 cut(s)	532, 717, 1178, 1771
ScaI	AGTACT	2 cut(s)	593, 1819
SchI	GAGTC	2 cut(s)	61, 1082
ScrFI	CCNGG	6 cut(s)	409, 493, 921, 981, 1167, 1580
SduI	GDGCHC	2 cut(s)	607, 1569
SfaNI	GCATC	6 cut(s)	454, 609, 895, 1000, 1347, 1750
SfcI	CTRYAG	3 cut(s)	543, 587, 1728
Sfr274I	CTCGAG	1 cut(s)	813
SfuI	TTCGAA	1 cut(s)	1803
SgrDI	CGTCGACG	1 cut(s)	227
SinI	GGWCC	2 cut(s)	532, 1178
SlaI	CTCGAG	1 cut(s)	813
SmiMI	CAYNNNNRTG	3 cut(s)	1020, 1319, 1572
SmlI	CTYRAG	2 cut(s)	694, 813
SmoI	CTYRAG	2 cut(s)	694, 813
SpeI	ACTAGT	1 cut(s)	451
SphI	GCATGC	2 cut(s)	362, 390
Sse9I	AATT	9 cut(s)	565, 1005, 1260, 1440, 1464, 1502, 1590, 1825, 1854
SseBI	AGGCCT	1 cut(s)	1617
SsiI	CCGC	9 cut(s)	177, 186, 198, 204, 208, 250, 371, 437, 1116
SspI	AATATT	1 cut(s)	1745
SspMI	CTAG	8 cut(s)	284, 294, 452, 968, 1604, 1749, 1815, 1870
SstI	GAGCTC	1 cut(s)	607
StuI	AGGCCT	1 cut(s)	1617
StyD4I	CCNGG	6 cut(s)	407, 491, 919, 979, 1165, 1578
TaaI	ACNGT	5 cut(s)	323, 487, 596, 754, 1209
TaiI	ACGT	2 cut(s)	234, 1810
TaqI	TCGA	5 cut(s)	165, 228, 344, 814, 1803
TaqII	GACCGA	1 cut(s)	1409
TasI	AATT	9 cut(s)	565, 1005, 1260, 1440, 1464, 1502, 1590, 1825, 1854
TatI	WGTACW	3 cut(s)	591, 746, 1817
TauI	GCSGC	2 cut(s)	374, 440
TfiI	GAWTC	6 cut(s)	77, 432, 503, 662, 809, 985
Tru1I	TTAA	2 cut(s)	1478, 1623
Tru9I	TTAA	2 cut(s)	1478, 1623
TscAI	CASTG	4 cut(s)	465, 1214, 1533, 1574
TseFI	GTSAC	2 cut(s)	1061, 1583
TseI	GCWGC	8 cut(s)	243, 383, 622, 790, 956, 1301, 1712, 1733
Tsp45I	GTSAC	2 cut(s)	1061, 1583
TspDTI	ATGAA	7 cut(s)	429, 1041, 1150, 1394, 1412, 1627, 1781
TspGWI	ACGGA	4 cut(s)	202, 362, 1170, 1365
TspRI	CASTG	4 cut(s)	465, 1214, 1533, 1574
Van91I	CCANNNNNTGG	2 cut(s)	931, 1102
VpaK11BI	GGWCC	2 cut(s)	532, 1178
XceI	RCATGY	3 cut(s)	362, 390, 1611
XcmI	CCANNNNNNNNNTGG	1 cut(s)	636
XhoI	CTCGAG	1 cut(s)	813
XmiI	GTMKAC	1 cut(s)	228
XmnI	GAANNNNTTC	2 cut(s)	1157, 1401
XspI	CTAG	8 cut(s)	284, 294, 452, 968, 1604, 1749, 1815, 1870
ZraI	GACGTC	1 cut(s)	232
ZrmI	AGTACT	2 cut(s)	593, 1819
Zsp2I	ATGCAT	2 cut(s)	1362, 1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.