MD14G1109700.v1.1
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007506
Physical Location & Seq
Reverse (-)
17301066 .. 17303044
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1109700.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCCATGACTCAATTGTGCTTGTCCGCTTCCTTGTGTTACAATTCTTGCTTTCCAAAAAATTCAAATCTTTCTCTCACACTTCCCTCAGCTTCACCTAAATGTAAGCCACTAGAAAATGGGGTGTTTAGCTTCAACACAATCCAATGCTCTGTGAGCCAGAATGGGACAATTGAGTATTCTGATAATGGGTTGGCTAGTTTTCCCGAAAAGCATTTGCATCGAAAGTCCGCTACTAGTTTGAAATCTCCGCCTCCTAGGCCTCGCCGGATAATCTTGGTTCGACATGGGCAGAGTGAAGGAAATGTAGATGAGAGTGCTTATACAAGAATTTCTGATCCAAAGATTGGACTAACAGAGAAAGGGTTGGCTGATGCTGAGCAATGTGGGAGGAGGATAAGGGAGATGATTGAGAGGGACAAAGTGCATAATTGGAAGGTGTACTTTTATGTGTCTCCTTATAGAAGGACAGTTCAAACACTGAAGGGTTTGGGTAGAGCGTTTGAGCGCTCAAGAATCGCCGGCATGAGAGAAGAGCCTCGGCTTAGAGAGCAGGATTTCGGGAATTTTCAGGATAGGGAGAAGATGAGAGTAGAGAAAGCGATTCGCATGCGTTATGGTCGTTTCTTTTATCGGTTTCCTAATGGAGAATCTGCAGCTGATGTTTATGATAGGATTACAGGATTTAGGGAAACACTGAGAGCAGATATCGATATAGGACGATTTCAACCACCGGGCGAGAGAAATCCAAACATCAACTTGGTGCTAGTTTCACACGGTCTAACGCTTAGAGTGTTCCTCATGAGATGGTACAAATGGACTGTGGAGCAATTTGAGAGGCTGAATAACTTTGGCAATGGAAGCATGGTTGTTATGGAAAGAGGCTATGGTGGAAGGTACAGCTTGTTAGTGCATCACACTGAAGAGGAGCTAAGACAGTTTGGATTGACAGATGAAATGCTAGTTGATCAAGAATGGCAAAAATATGCAAAACCCGGGGAGCTGAACTACGATTGCCCGATGCTAAATTCCTTCTTCACCCACTTCGGTGACGATGACGATGATGGTGATCGTGCAGCCGTTAGCACACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

365

Amino Acids

42.0

Weight (kDa)

8.74

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 91 - 300 2.1e-29 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 347
AciI CCGC 3 cut(s) 27, 231, 251
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 61, 330, 565, 1027
AcuI CTGAAG 2 cut(s) 503, 942
AfaI GTAC 3 cut(s) 443, 812, 899
AfeI AGCGCT 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 5 cut(s) 66, 136, 244, 476, 728
AhlI ACTAGT 1 cut(s) 236
AleI CACNNNNGTG 1 cut(s) 1047
AluBI AGCT 6 cut(s) 92, 132, 659, 903, 931, 1003
AluI AGCT 6 cut(s) 92, 132, 659, 903, 931, 1003
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 995
Aor51HI AGCGCT 1 cut(s) 509
AoxI GGCC 2 cut(s) 3, 260
ApeKI GCWGC 2 cut(s) 656, 1076
ApoI RAATTY 4 cut(s) 61, 330, 565, 1027
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 1 cut(s) 510
AsuC2I CCSGG 3 cut(s) 735, 996, 997
AsuHPI GGTGA 4 cut(s) 87, 1030, 1061, 1079
AvaI CYCGRG 1 cut(s) 995
AvrII CCTAGG 1 cut(s) 257
BalI TGGCCA 1 cut(s) 5
BbvCI CCTCAGC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 668, 1088
BccI CCATC 2 cut(s) 801, 1058
BceAI ACGGC 1 cut(s) 1064
BcgI CGANNNNNNTGC 4 cut(s) 203, 237, 592, 626
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 3 cut(s) 735, 996, 997
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 7 cut(s) 113, 198, 237, 258, 767, 962, 1093
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 511
BglI GCCNNNNNGGC 1 cut(s) 259
BisI GCNGC 2 cut(s) 657, 1077
BlnI CCTAGG 1 cut(s) 257
BlpI GCTNAGC 1 cut(s) 378
BlsI GCNGC 2 cut(s) 658, 1078
Bme1390I CCNGG 3 cut(s) 735, 996, 997
BmeT110I CYCGRG 1 cut(s) 995
BmrFI CCNGG 3 cut(s) 735, 996, 997
BmsI GCATC 4 cut(s) 229, 364, 922, 1011
BplI GAGNNNNNCTC 2 cut(s) 783, 815
Bpu10I CCTNAGC 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 496
BpuMI CCSGG 3 cut(s) 735, 996, 997
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 1068
BsaJI CCNNGG 4 cut(s) 257, 539, 995, 996
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 521
Bse3DI GCAATG 2 cut(s) 389, 862
Bse8I GATNNNNATC 1 cut(s) 1068
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 4 cut(s) 257, 539, 995, 996
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 2 cut(s) 389, 862
BseMII CTCAG 3 cut(s) 102, 369, 689
BseRI GAGGAG 2 cut(s) 406, 941
BseXI GCAGC 2 cut(s) 668, 1088
BsgI GTGCAG 1 cut(s) 1095
BshFI GGCC 2 cut(s) 5, 262
BshVI ATCGAT 1 cut(s) 711
BsiHKCI CYCGRG 1 cut(s) 995
BsiSI CCGG 4 cut(s) 268, 522, 734, 996
BslFI GGGAC 2 cut(s) 181, 431
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 2 cut(s) 181, 431
BsnI GGCC 2 cut(s) 5, 262
BsoBI CYCGRG 1 cut(s) 995
Bsp143I GATC 3 cut(s) 337, 967, 1069
Bsp1720I GCTNAGC 1 cut(s) 378
BspACI CCGC 3 cut(s) 27, 231, 251
BspANI GGCC 2 cut(s) 5, 262
BspCNI CTCAG 3 cut(s) 101, 370, 690
BspDI ATCGAT 1 cut(s) 711
BspHI TCATGA 1 cut(s) 801
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 528
BsrDI GCAATG 2 cut(s) 389, 862
BsrFI RCCGGY 1 cut(s) 521
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 4 cut(s) 257, 539, 995, 996
BssMI GATC 3 cut(s) 337, 967, 1069
BssT1I CCWWGG 1 cut(s) 257
Bst4CI ACNGT 4 cut(s) 472, 779, 823, 939
Bst6I CTCTTC 2 cut(s) 528, 918
BstC8I GCNNGC 2 cut(s) 523, 611
BstDEI CTNAG 6 cut(s) 88, 378, 545, 698, 788, 932
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 3 cut(s) 340, 970, 1072
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 337, 967, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 156, 259, 550, 861
BstNSI RCATGY 1 cut(s) 613
BstSCI CCNGG 3 cut(s) 733, 994, 995
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 2 cut(s) 668, 1088
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 2 cut(s) 5, 262
BsuTUI ATCGAT 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 479, 695, 918
Cac8I GCNNGC 2 cut(s) 523, 611
CciI TCATGA 1 cut(s) 801
CfoI GCGC 1 cut(s) 510
Cfr10I RCCGGY 1 cut(s) 521
Cfr9I CCCGGG 1 cut(s) 995
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 3 cut(s) 442, 811, 898
CviAII CATG 6 cut(s) 7, 287, 526, 610, 802, 865
CviQI GTAC 3 cut(s) 442, 811, 898
DdeI CTNAG 6 cut(s) 88, 378, 545, 698, 788, 932
DpnI GATC 3 cut(s) 339, 969, 1071
DpnII GATC 3 cut(s) 337, 967, 1069
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 528, 918
EarI CTCTTC 2 cut(s) 528, 918
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 262
Eco32I GATATC 1 cut(s) 709
Eco47III AGCGCT 1 cut(s) 509
Eco57I CTGAAG 2 cut(s) 503, 942
Eco88I CYCGRG 1 cut(s) 995
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 6 cut(s) 10, 290, 529, 613, 805, 868
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 2 cut(s) 181, 431
FatI CATG 6 cut(s) 6, 286, 525, 609, 801, 864
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 2 cut(s) 657, 1077
Fsp4HI GCNGC 2 cut(s) 657, 1077
FspBI CTAG 7 cut(s) 113, 198, 237, 258, 767, 962, 1093
GlaI GCGC 1 cut(s) 509
GluI GCNGC 2 cut(s) 657, 1077
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 2 cut(s) 5, 262
HapII CCGG 4 cut(s) 268, 522, 734, 996
HhaI GCGC 1 cut(s) 510
Hin1II CATG 6 cut(s) 10, 290, 529, 613, 805, 868
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HinfI GANTC 4 cut(s) 10, 516, 604, 650
HpaII CCGG 4 cut(s) 268, 522, 734, 996
HphI GGTGA 4 cut(s) 87, 1030, 1061, 1079
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 2 cut(s) 184, 337
Hpy188III TCNNGA 6 cut(s) 206, 513, 562, 572, 802, 971
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 6 cut(s) 293, 430, 459, 478, 888, 1042
HpyCH4III ACNGT 4 cut(s) 472, 779, 823, 939
HpyCH4V TGCA 6 cut(s) 220, 427, 656, 913, 989, 1076
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 259, 550, 861
HpyF3I CTNAG 6 cut(s) 88, 378, 545, 698, 788, 932
Hsp92II CATG 6 cut(s) 10, 290, 529, 613, 805, 868
HspAI GCGC 1 cut(s) 508
KroI GCCGGC 1 cut(s) 521
KroNI GCCGGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 3 cut(s) 337, 967, 1069
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 3 cut(s) 826, 928, 1000
LpnPI CCDG 8 cut(s) 173, 281, 535, 539, 557, 666, 747, 1009
Lsp1109I GCAGC 2 cut(s) 668, 1088
LweI GCATC 4 cut(s) 229, 364, 922, 1011
MaeI CTAG 7 cut(s) 113, 198, 237, 258, 767, 962, 1093
MaeIII GTNAC 2 cut(s) 38, 1049
MalI GATC 3 cut(s) 339, 969, 1071
MboI GATC 3 cut(s) 337, 967, 1069
MboII GAAGA 4 cut(s) 545, 595, 935, 1027
MfeI CAATTG 2 cut(s) 14, 171
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 14, 43, 61, 171, 330, 430, 565, 830, 1027
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 4
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 521
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 100, 1047
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 4 cut(s) 268, 522, 734, 996
MspR9I CCNGG 3 cut(s) 735, 996, 997
MunI CAATTG 2 cut(s) 14, 171
MwoI GCNNNNNNNGC 4 cut(s) 156, 259, 550, 861
NaeI GCCGGC 1 cut(s) 523
NciI CCSGG 3 cut(s) 735, 996, 997
NdeII GATC 3 cut(s) 337, 967, 1069
NgoMIV GCCGGC 1 cut(s) 521
NlaIII CATG 6 cut(s) 10, 290, 529, 613, 805, 868
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 1049
NspI RCATGY 1 cut(s) 613
OliI CACNNNNGTG 1 cut(s) 1047
PaeI GCATGC 1 cut(s) 613
PagI TCATGA 1 cut(s) 801
PceI AGGCCT 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 528
PdiI GCCGGC 1 cut(s) 523
PfeI GAWTC 3 cut(s) 516, 604, 650
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 2 cut(s) 658, 1078
PleI GAGTC 1 cut(s) 4
PpsI GAGTC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 3 cut(s) 443, 812, 899
RsaNI GTAC 3 cut(s) 442, 811, 898
RseI CAYNNNNRTG 2 cut(s) 100, 1047
SapI GCTCTTC 1 cut(s) 528
SatI GCNGC 2 cut(s) 657, 1077
Sau3AI GATC 3 cut(s) 337, 967, 1069
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 3 cut(s) 735, 996, 997
SetI ASST 9 cut(s) 94, 100, 134, 441, 661, 899, 905, 933, 1005
SfaNI GCATC 4 cut(s) 229, 364, 922, 1011
SfcI CTRYAG 1 cut(s) 654
SmaI CCCGGG 1 cut(s) 997
SmiMI CAYNNNNRTG 2 cut(s) 100, 1047
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SpeI ACTAGT 1 cut(s) 236
SphI GCATGC 1 cut(s) 613
Sse9I AATT 9 cut(s) 14, 43, 61, 171, 330, 430, 565, 830, 1027
SseBI AGGCCT 1 cut(s) 262
SsiI CCGC 3 cut(s) 27, 231, 251
SspMI CTAG 7 cut(s) 113, 198, 237, 258, 767, 962, 1093
StuI AGGCCT 1 cut(s) 262
StyD4I CCNGG 3 cut(s) 733, 994, 995
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 4 cut(s) 472, 779, 823, 939
TaqI TCGA 3 cut(s) 223, 283, 711
TasI AATT 9 cut(s) 14, 43, 61, 171, 330, 430, 565, 830, 1027
TatI WGTACW 1 cut(s) 441
TfiI GAWTC 3 cut(s) 516, 604, 650
TscAI CASTG 3 cut(s) 486, 702, 925
TseFI GTSAC 1 cut(s) 1049
TseI GCWGC 2 cut(s) 656, 1076
Tsp45I GTSAC 1 cut(s) 1049
TspDTI ATGAA 1 cut(s) 969
TspMI CCCGGG 1 cut(s) 995
TspRI CASTG 3 cut(s) 486, 702, 925
Van91I CCANNNNNTGG 1 cut(s) 347
XapI RAATTY 4 cut(s) 61, 330, 565, 1027
XceI RCATGY 1 cut(s) 613
XmaI CCCGGG 1 cut(s) 995
XmaJI CCTAGG 1 cut(s) 257
XspI CTAG 7 cut(s) 113, 198, 237, 258, 767, 962, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.