pycom06g08450
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007506
Physical Location & Seq
Reverse (-)
13620866 .. 13622499
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08450.1

Sequence Viewer

Length: 1113 bp
ATGGCAATGGCTCAATTTTGCTTCTCTGCTTGCCTGTGTTACAATTCTTGCTTTCCAAGAAATTCAAATCTTTCTCTCACACTTCTGTCGGGTTCACCTAAACGTAAGCCACTAGAAAATGGGGTGCTTAGTTTCAACACAATCCAATGCCGTGTGAACCATAATGGGACAGTTGAGCATTCTTATAATGGGTTGTCTAGCTTTCCCGAGAAGCATTTGCATGGAAGGTCAGCTAGTGGTTCGAAATCTCCACCCCCTAGGCCTCGCCGGATAATCTTGGTTCGACATGGGCAGAGTGAAGGAAATGTAGATGAGAGTGCTTATACAAGTGCCAACGAAATTTCTGATCCAAAGATTGGACTGACAGGGAAAGGGTTGGCTGATGCTGAGGATTGTGGGAGGAGGATAAGGGAGATGATTGAGGGGGACAAAGCGCATAATTGGAAGGTCTACTTTTATGTATCTCCTTATAGAAGGACAGTCGAAACATTGAAGGGTTTGGGTAGAGCATTTGAGCGCTCAAGAATCGCCGGCATGAGAGTAGAGCCTCGGCTTAGAGAGCAGGATTTCGGGAATTTTCAGGATAGGGAGAAGATGAGAGTAGAGAAAGCGATTCGCATGCTTTATGGGCGTTTCTTTTATCGGTTTCCTAATGGGGAATCTGCAGCTGATGTTTATGATAGGATTACAGGATTTAGAGAAACACTAAGATCAGATATCGATATAGGACGATTTCAGCCACCGGGCGAGAGAAATCCAAACATCAACTTGGTGCTAGTTTCACATGGTCTAACGCTTAGAGTGTTCCTCATGAGATGGTACAAATGGACAGTGGAGCAATTTGAGAGGCTGAATAACCTTGGTAATGGAGGCATGGTTGTCATGGAAAGAGGCTATGGTGGAAGGTACAGTTTGTTAATGCACCACACTGAAGAGGAGCTAAGACAGTTTGGAATGACAGATGAAATGCTCGTTGATCAAGAATGGCAAAAATATGCAAAACCTGGGGAGCTGAACTACGATTGCCCGATGCTAAATTCCTTCTTCACCCACTTCGCTAATGGTGACGATGATGGTAATCGCGCAGCCGTTAGCCTTGGAGCTGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

371

Amino Acids

42.25

Weight (kDa)

8.75

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 91 - 303 2.1e-25 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 356
AccI GTMKAC 1 cut(s) 450
AccII CGCG 1 cut(s) 1083
AclWI GGATC 1 cut(s) 341
AcsI RAATTY 4 cut(s) 61, 339, 574, 1036
AcuI CTGAAG 1 cut(s) 951
AfaI GTAC 2 cut(s) 821, 908
AfeI AGCGCT 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 3 cut(s) 66, 136, 493
AjnI CCWGG 1 cut(s) 1003
AluBI AGCT 6 cut(s) 201, 233, 668, 940, 1012, 1103
AluI AGCT 6 cut(s) 201, 233, 668, 940, 1012, 1103
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 206
Aor51HI AGCGCT 1 cut(s) 518
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 665, 1085
ApoI RAATTY 4 cut(s) 61, 339, 574, 1036
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 3 cut(s) 436, 519, 1085
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 3 cut(s) 87, 1039, 1076
AsuII TTCGAA 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 206
AvrII CCTAGG 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 677, 1097
BccI CCATC 2 cut(s) 810, 1067
BceAI ACGGC 2 cut(s) 135, 1073
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 1 cut(s) 1005
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 1 cut(s) 744
BfaI CTAG 5 cut(s) 113, 198, 234, 258, 776
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 520
BisI GCNGC 2 cut(s) 666, 1086
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 2 cut(s) 667, 1087
Bme1390I CCNGG 2 cut(s) 744, 1005
BmeT110I CYCGRG 1 cut(s) 206
BmrFI CCNGG 2 cut(s) 744, 1005
BmsI GCATC 2 cut(s) 373, 1020
BplI GAGNNNNNCTC 2 cut(s) 792, 824
Bpu10I CCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 744
Bsa29I ATCGAT 1 cut(s) 720
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 5 cut(s) 257, 548, 859, 1004, 1096
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 1 cut(s) 1005
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 5 cut(s) 257, 548, 859, 1004, 1096
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 415, 950
BseXI GCAGC 2 cut(s) 677, 1097
Bsh1236I CGCG 1 cut(s) 1083
BshFI GGCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 268, 531, 743
BslFI GGGAC 2 cut(s) 181, 440
BslI CCNNNNNNNGG 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 181, 440
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 242
Bsp143I GATC 3 cut(s) 346, 710, 976
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 379
BspDI ATCGAT 1 cut(s) 720
BspFNI CGCG 1 cut(s) 1083
BspHI TCATGA 1 cut(s) 810
BspMAI CTGCAG 1 cut(s) 667
BspPI GGATC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 530
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 5 cut(s) 257, 548, 859, 1004, 1096
BssMI GATC 3 cut(s) 346, 710, 976
BssT1I CCWWGG 3 cut(s) 257, 859, 1096
Bst2UI CCWGG 1 cut(s) 1005
Bst4CI ACNGT 5 cut(s) 172, 481, 832, 911, 948
Bst6I CTCTTC 1 cut(s) 927
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 31, 532, 620
BstDEI CTNAG 6 cut(s) 128, 387, 554, 707, 797, 941
BstFNI CGCG 1 cut(s) 1083
BstH2I RGCGCY 1 cut(s) 520
BstHHI GCGC 3 cut(s) 436, 519, 1085
BstKTI GATC 3 cut(s) 349, 713, 979
BstMBI GATC 3 cut(s) 346, 710, 976
BstMWI GCNNNNNNNGC 2 cut(s) 559, 628
BstNI CCWGG 1 cut(s) 1005
BstNSI RCATGY 1 cut(s) 622
BstSCI CCNGG 2 cut(s) 742, 1003
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 1 cut(s) 1083
BstV1I GCAGC 2 cut(s) 677, 1097
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 837, 927
Cac8I GCNNGC 3 cut(s) 31, 532, 620
CciI TCATGA 1 cut(s) 810
CfoI GCGC 3 cut(s) 436, 519, 1085
Cfr10I RCCGGY 1 cut(s) 530
ClaI ATCGAT 1 cut(s) 720
Csp6I GTAC 2 cut(s) 820, 907
CviAII CATG 9 cut(s) 221, 287, 535, 619, 785, 811, 874, 883, 1110
CviQI GTAC 2 cut(s) 820, 907
DdeI CTNAG 6 cut(s) 128, 387, 554, 707, 797, 941
DpnI GATC 3 cut(s) 348, 712, 978
DpnII GATC 3 cut(s) 346, 710, 976
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
Eco130I CCWWGG 3 cut(s) 257, 859, 1096
Eco147I AGGCCT 1 cut(s) 262
Eco32I GATATC 1 cut(s) 718
Eco47III AGCGCT 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 951
Eco88I CYCGRG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 1003
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 3 cut(s) 257, 859, 1096
ErhI CCWWGG 3 cut(s) 257, 859, 1096
FaeI CATG 9 cut(s) 224, 290, 538, 622, 788, 814, 877, 886, 1113
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 2 cut(s) 181, 440
FatI CATG 9 cut(s) 220, 286, 534, 618, 784, 810, 873, 882, 1109
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 666, 1086
Fsp4HI GCNGC 2 cut(s) 666, 1086
FspBI CTAG 5 cut(s) 113, 198, 234, 258, 776
GlaI GCGC 3 cut(s) 435, 518, 1084
GluI GCNGC 2 cut(s) 666, 1086
HaeII RGCGCY 1 cut(s) 520
HaeIII GGCC 1 cut(s) 262
HapII CCGG 3 cut(s) 268, 531, 743
HhaI GCGC 3 cut(s) 436, 519, 1085
Hin1II CATG 9 cut(s) 224, 290, 538, 622, 788, 814, 877, 886, 1113
Hin6I GCGC 3 cut(s) 434, 517, 1083
HinP1I GCGC 3 cut(s) 434, 517, 1083
HinfI GANTC 3 cut(s) 525, 613, 659
HpaII CCGG 3 cut(s) 268, 531, 743
HphI GGTGA 3 cut(s) 87, 1039, 1076
Hpy166II GTNNAC 3 cut(s) 95, 157, 451
Hpy188I TCNGA 2 cut(s) 346, 715
Hpy188III TCNNGA 6 cut(s) 206, 522, 571, 581, 811, 980
Hpy8I GTNNAC 3 cut(s) 95, 157, 451
HpyAV CCTTC 7 cut(s) 219, 293, 439, 468, 487, 897, 1051
HpyCH4III ACNGT 5 cut(s) 172, 481, 832, 911, 948
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 4 cut(s) 220, 665, 922, 998
HpyF10VI GCNNNNNNNGC 2 cut(s) 559, 628
HpyF3I CTNAG 6 cut(s) 128, 387, 554, 707, 797, 941
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 9 cut(s) 224, 290, 538, 622, 788, 814, 877, 886, 1113
HspAI GCGC 3 cut(s) 434, 517, 1083
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 3 cut(s) 346, 710, 976
LmnI GCTCC 4 cut(s) 835, 937, 1009, 1100
Lsp1109I GCAGC 2 cut(s) 677, 1097
LweI GCATC 2 cut(s) 373, 1020
MaeI CTAG 5 cut(s) 113, 198, 234, 258, 776
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 2 cut(s) 38, 1064
MalI GATC 3 cut(s) 348, 712, 978
MboI GATC 3 cut(s) 346, 710, 976
MboII GAAGA 3 cut(s) 604, 944, 1036
MluCI AATT 8 cut(s) 14, 43, 61, 339, 439, 574, 839, 1036
MroNI GCCGGC 1 cut(s) 530
MseI TTAA 1 cut(s) 917
MslI CAYNNNNRTG 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 3 cut(s) 268, 531, 743
MspR9I CCNGG 2 cut(s) 744, 1005
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 1005
MvnI CGCG 1 cut(s) 1083
MwoI GCNNNNNNNGC 2 cut(s) 559, 628
NaeI GCCGGC 1 cut(s) 532
NciI CCSGG 1 cut(s) 744
NdeII GATC 3 cut(s) 346, 710, 976
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 9 cut(s) 224, 290, 538, 622, 788, 814, 877, 886, 1113
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 1064
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 622
PagI TCATGA 1 cut(s) 810
PceI AGGCCT 1 cut(s) 262
PctI GAATGC 1 cut(s) 178
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 3 cut(s) 525, 613, 659
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 2 cut(s) 667, 1087
PsiI TTATAA 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 1003
PspGI CCWGG 1 cut(s) 1003
PstI CTGCAG 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 821, 908
RsaNI GTAC 2 cut(s) 820, 907
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 1 cut(s) 917
SatI GCNGC 2 cut(s) 666, 1086
Sau3AI GATC 3 cut(s) 346, 710, 976
ScrFI CCNGG 2 cut(s) 744, 1005
SfaNI GCATC 2 cut(s) 373, 1020
SfcI CTRYAG 1 cut(s) 663
SfuI TTCGAA 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
SphI GCATGC 1 cut(s) 622
Sse9I AATT 8 cut(s) 14, 43, 61, 339, 439, 574, 839, 1036
SseBI AGGCCT 1 cut(s) 262
SspMI CTAG 5 cut(s) 113, 198, 234, 258, 776
StuI AGGCCT 1 cut(s) 262
StyD4I CCNGG 2 cut(s) 742, 1003
StyI CCWWGG 3 cut(s) 257, 859, 1096
TaaI ACNGT 5 cut(s) 172, 481, 832, 911, 948
TaiI ACGT 1 cut(s) 106
TaqI TCGA 4 cut(s) 242, 283, 483, 720
TasI AATT 8 cut(s) 14, 43, 61, 339, 439, 574, 839, 1036
TfiI GAWTC 3 cut(s) 525, 613, 659
Tru1I TTAA 1 cut(s) 917
Tru9I TTAA 1 cut(s) 917
TscAI CASTG 2 cut(s) 837, 934
TseFI GTSAC 1 cut(s) 1064
TseI GCWGC 2 cut(s) 665, 1085
Tsp45I GTSAC 1 cut(s) 1064
TspDTI ATGAA 1 cut(s) 978
TspRI CASTG 2 cut(s) 837, 934
Van91I CCANNNNNTGG 1 cut(s) 356
XapI RAATTY 4 cut(s) 61, 339, 574, 1036
XceI RCATGY 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 1058
XmaJI CCTAGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 5 cut(s) 113, 198, 234, 258, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.