MD14G1144600.v1.1

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004359
Physical Location & Seq
Forward (+)
23787200 .. 23793412
6213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1144600.v1.1.491

Sequence Viewer

Length: 2985 bp
ATGGCTTCATCAACAGCTTCCACAGGATGGTACAGAGGGAGAGTGAAGGCTGTGCCTTCAGGGGACTCCTTGGTCGTCATGGCCCTCACAGCCAACAAGGCCGGACCTCCACCCGAAAGAACCATCACTCTGTCGTCACTTATGGCTCCGAAACTGGCTCGTAGAGATGGCCAGGATGAACCATTTGCATGGGGCAGCAGGGAGTTTCTGAGAAAACTCTGCATAGGAAAGGAGGTGGCTTTCAGAGTGGACTATGTTGTACAACAGATAGGGCGTGAATTCGGCTCGGTTTTCCTTGGTGACAAGAATCTTGCTATGCTGGTTGTTGCTGAAGGCTGGGCAAAGGTCAAGGAGGGTAAGCAAGGTCCACAGAAAGCAGAAGCGAGTCCTTACATTGCAGAGCTGCTACGTCTCCAAGAGCAAGCTAGGACTGAGGGCCTTGGTCTTCATAGCAAGGTTCCTGGTGCTGGTGAAGCATCCAAAAGGAATCTACCTCCCTCTGCTATTGGGAATGCCAGCAACTTAGATGCTGTGAGTCTGTTGGCTGAAAATAAGGGCAGGTCAATGGAATGTATTGTCGAGCAGGTTCGTGATGGAAGCACAGTTAGGGCCTACTTGCTTCCGGATTTTCAGTTTGTCCAAGTATTCGTTGCAGGAATTCAGGCCCCATCGGTGGGAAGGAGACCTGTTACATCAGACATTGTTGCTGAACCAGAAACAACTTCCAATAAAACAAATGGAGATGTTTCTTCTGAACCTCGAGGACCTCTAACTTCTGCTCAAAGGATTTTAGCTTCAACAGCATCGTCTGTTGAAGTTTCTGCTGATCCATTTGCATTGGAAGCTAAACATTTTACAGAAATTCGTGTGTTGCATAGAGATGTCCGCATCGTTCTGGAAGGCAATGACAAGTTCAGTAATTTGATTGGGTCAGTATATTACTCCGATGGGGACTCGGCAAAAGACTTGGCCCTCGAGCTTGTTGAAAATGGTTATGCAAAGTACGTTGAATGGAGTGCAACCACGTTGGAAGATGATGTGAAGCGGCGGCTGAAGACTGCAGAGCTTCAAGCGAAAAAAAGCAAGTTGAGGATTTGGACAAACTACACACCCCCTCCTACAAATTCAAAAGCAATTCATAACCAGAATTTCACTGGAAAGGTTGTGGAGGTTGTAAGCGGGGACTGCGTGATTGTTGCTGATGATTCTGTTCCATTTGGCAGTCCACTAGCAGAGAGGCGAGTTAATCTTTCAAGTATTAGGTGTCCGAAAATGGGCAATCCTCGTAGGGAAGAAAAGCCGGCTCCATATGCCCGTGAAGCCAAAGAGTTCCTGAGAACACGCCTTATTGGACGTCAAGTAAATGTTCAAATGGAGTATTCAAGGAAGGTCGTCCCTGGAGAGGGAGCTGCACCTACTTCTGGACCTGCCGATTCAAGGGTAATGGATTTTGGAACAGTTTTCCTTGCATCTCCTACTAAGGCTGAGGGTGATGACGCCCCAGCACCTGCTTCATCTGCGCCTGCCAGTCAGCAGGCTGGTGTGAATGTTGCTGAGTTGGTGGTTTCACGTGGCTTTGGCACTGTTATTAGACATCGAGATTTTGAGGAGAGATCAAACTATTATGATGCCCTTATGTCAGCAGAAACACGTGCTACTGCTGGAAAGAAAGGAATACATTCTACCAAGGAACCTCCAGTCATGCACATTACAGACTTGACAGTGGCATCAGCAAAGAAAGCCAGGGACTTCTTTCCATTCTTACAGAAAAGGAGGAAAATTCCTGCTGTTGTGGAATATGTCCTCAGTGGGCATCGTTTTAAATTATTGATTCCCAAGGAAACATGCAGCATTGCCTTCGCATTCTCTGGTGTCAGATGTCCTGGTCGTGAGGAGCCATATTCAGACGAAGCAATTGCGCTCATGAGGCGAAGAATTATGCAGAGAGATGTTGAGATTGAAGTCGAGACTGTTGATAGAACCGGGACCTTCTTGGGATCTTTATGGGAGTCAAAGACCAATGTGGCGGTTGCCCTTGTTGAAGCTGGCCTTGCAAAATTTCAAAATTCTTTTGGCGGTGAAATCCCTGATGGGCACCTTCTTGAAAAAGCTGAGGAATCTGCGAAAAAGAAGAAACTGAAAATCTGGGAGAACTATGTTGAAGGAGAGGAGGTTCCCAACGGTTCAGCCGTTGACAACACGAAACAGAAAGAAGTGCTAAAGGTAGCGGTGACAGAGGTTTTGGGAAGCGGTAAATTTTATGTTCAGACAGTTGGGGATCAGAAAATTGCCTCAATTCAGCAACAGCTTGCATCTTTGAATCTTCAAGAAGCTCCCGTAATTGGTGCTTATAATCCTAAGAAGGGTGACATTGTCCTTGCTCAGTTTAGTGCAGATAATTCTTGGAACCGCGCAATGATTGTCAATGCACCTCGAGGAGCTGTGGAATCCCCTAAAGACAAGTTTGAAGTTTTCTACATGGATTATGGTAACCAAGAGGTTGTCCCTTACAGTCAGATACGGCCTCTTGACCCTTCGGTTTCCTCTGCACCCGGTCTTGCTCAATTATGCAGCCTTGCATACGTTAAAGTCCCGGGCTTGGAGGAGGACTTTGGTCAAGAAGCAGCAGAGTATTTAAGTGAGCACGCATTAAACAGTGCCAAAGAATTTAGGGCCATGATTGAGGAAAGGGATCTTTCAGGGGGAAAAGTTAAGGGTCAGGGAACCGGGCCAGTGCTTCTTGTGACTCTTGTCGCTGTGGATACTGAGATCAGCTTGAATGCCGCCATGCTTCAGGAAGGACTTGCAAGGCTAGAGAAAATGAAGAAACGTGAAACCAAGGAGAGGAAGACTGCAATCGAGAACTTGGAAAAGTTCCAGGAAGAAGCAAGGACTGATAGGCGGGGGATGTGGCGCTACGGAGACATCCAGTCCGATGATGAGGACGTTCCTCCGGTTAGGAAGGCCGCTGCTGGCAAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

995

Amino Acids

108.77

Weight (kDa)

8.15

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 41 - 151 3.3e-09 Staphylococcal nuclease homologue
SNase PF00565 273 - 367 2.7e-14 Staphylococcal nuclease homologue
SNase PF00565 622 - 718 7.3e-15 Staphylococcal nuclease homologue
TUDOR PF00567 737 - 860 4.9e-22 Tudor domain
SNase PF00565 867 - 972 4.3e-08 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2352
AarI CACCTGC 1 cut(s) 1516
AasI GACNNNNNNGTC 1 cut(s) 71
AatII GACGTC 1 cut(s) 1357
AbsI CCTCGAGG 2 cut(s) 759, 2433
Acc36I ACCTGC 4 cut(s) 549, 574, 1435, 1516
AccB1I GGYRCC 1 cut(s) 2094
AccB7I CCANNNNNTGG 1 cut(s) 27
AccII CGCG 1 cut(s) 2412
AccIII TCCGGA 1 cut(s) 622
AclWI GGATC 4 cut(s) 821, 2005, 2286, 2700
AcoI YGGCCR 1 cut(s) 169
AcuI CTGAAG 4 cut(s) 42, 351, 1073, 2777
AcvI CACGTG 2 cut(s) 1571, 1652
AcyI GRCGYC 2 cut(s) 1354, 1497
AfaI GTAC 3 cut(s) 32, 261, 1004
AflIII ACRYGT 1 cut(s) 1649
AhdI GACNNNNNGTC 1 cut(s) 2929
AjnI CCWGG 6 cut(s) 171, 460, 1396, 1742, 1882, 2877
AloI GAACNNNNNNTCC 2 cut(s) 2157, 2189
Alw21I GWGCWC 1 cut(s) 2646
Alw26I GTCTC 4 cut(s) 416, 676, 1961, 2916
AlwI GGATC 4 cut(s) 821, 2005, 2286, 2700
AlwNI CAGNNNCTG 1 cut(s) 1508
Ama87I CYCGRG 4 cut(s) 759, 974, 2433, 2593
Aor13HI TCCGGA 1 cut(s) 622
ApeKI GCWGC 7 cut(s) 195, 403, 1409, 1848, 2571, 2624, 2969
ArsI GACNNNNNNTTYG 2 cut(s) 2224, 2256
Asp700I GAANNNNTTC 1 cut(s) 1678
AspLEI GCGC 4 cut(s) 1522, 1921, 2414, 2916
AsuC2I CCSGG 5 cut(s) 1984, 2553, 2594, 2595, 2728
AsuHPI GGTGA 6 cut(s) 311, 482, 1502, 2090, 2242, 2378
AvaI CYCGRG 4 cut(s) 759, 974, 2433, 2593
AvaII GGWCC 5 cut(s) 104, 365, 764, 1424, 1986
BaeGI GKGCMC 1 cut(s) 2097
BalI TGGCCA 1 cut(s) 171
BanI GGYRCC 1 cut(s) 2094
BbrPI CACGTG 2 cut(s) 1571, 1652
BbsI GAAGAC 3 cut(s) 437, 1061, 2855
Bbv12I GWGCWC 1 cut(s) 2646
BbvCI CCTCAGC 2 cut(s) 1485, 2112
BbvI GCAGC 7 cut(s) 207, 390, 1396, 1860, 2583, 2636, 2956
BccI CCATC 7 cut(s) 21, 131, 161, 587, 676, 941, 2084
BceAI ACGGC 2 cut(s) 2174, 2537
BcgI CGANNNNNNTGC 4 cut(s) 1840, 1874, 1898, 1932
BciT130I CCWGG 6 cut(s) 173, 462, 1398, 1744, 1884, 2879
BciVI GTATCC 1 cut(s) 2755
BcnI CCSGG 5 cut(s) 1984, 2553, 2594, 2595, 2728
BcoDI GTCTC 4 cut(s) 416, 676, 1961, 2916
BfaI CTAG 3 cut(s) 426, 1229, 2813
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 1 cut(s) 2917
BfuAI ACCTGC 4 cut(s) 549, 574, 1435, 1516
BfuI GTATCC 1 cut(s) 2755
BglI GCCNNNNNGGC 1 cut(s) 98
Bme18I GGWCC 5 cut(s) 104, 365, 764, 1424, 1986
BmeRI GACNNNNNGTC 1 cut(s) 2929
BmeT110I CYCGRG 4 cut(s) 759, 974, 2433, 2593
BmsI GCATC 9 cut(s) 485, 517, 812, 897, 1478, 1618, 1736, 1822, 2321
BoxI GACNNNNGTC 2 cut(s) 2613, 2750
BpiI GAAGAC 3 cut(s) 437, 1061, 2855
BpmI CTGGAG 2 cut(s) 1419, 1680
Bpu10I CCTNAGC 2 cut(s) 1485, 2112
BpuMI CCSGG 5 cut(s) 1984, 2553, 2594, 2595, 2728
BsaAI YACGTR 2 cut(s) 1571, 1652
BsaHI GRCGYC 2 cut(s) 1354, 1497
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 9 cut(s) 69, 295, 439, 1396, 1686, 1743, 1836, 2593, 2838
BsaWI WCCGGW 2 cut(s) 622, 2953
BsaXI ACNNNNNCTCC 4 cut(s) 1099, 1129, 2157, 2187
Bse118I RCCGGY 1 cut(s) 1300
Bse1I ACTGG 6 cut(s) 159, 1159, 1527, 1697, 2732, 2929
Bse3DI GCAATG 4 cut(s) 393, 910, 1851, 2421
BseAI TCCGGA 1 cut(s) 622
BseBI CCWGG 6 cut(s) 173, 462, 1398, 1744, 1884, 2879
BseDI CCNNGG 9 cut(s) 69, 295, 439, 1396, 1686, 1743, 1836, 2593, 2838
BseGI GGATG 5 cut(s) 32, 181, 476, 2913, 2925
BseMI GCAATG 4 cut(s) 393, 910, 1851, 2421
BseMII CTCAG 9 cut(s) 200, 423, 1325, 1476, 1545, 1819, 2103, 2396, 2757
BseNI ACTGG 6 cut(s) 159, 1159, 1527, 1697, 2732, 2929
BseRI GAGGAG 5 cut(s) 1622, 1907, 2183, 2451, 2618
BseSI GKGCMC 1 cut(s) 2097
BseXI GCAGC 7 cut(s) 207, 390, 1396, 1860, 2583, 2636, 2956
BseYI CCCAGC 2 cut(s) 336, 1501
BsgI GTGCAG 3 cut(s) 1395, 2412, 2532
Bsh1236I CGCG 1 cut(s) 2412
BshNI GGYRCC 1 cut(s) 2094
BsiHKAI GWGCWC 1 cut(s) 2646
BsiHKCI CYCGRG 4 cut(s) 759, 974, 2433, 2593
BsiSI CCGG 8 cut(s) 102, 623, 1301, 1983, 2553, 2594, 2727, 2954
BslFI GGGAC 8 cut(s) 77, 965, 1196, 1379, 1760, 1999, 2489, 2576
BsmAI GTCTC 4 cut(s) 416, 676, 1961, 2916
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 8 cut(s) 77, 965, 1196, 1379, 1760, 1999, 2489, 2576
BsmI GAATGC 3 cut(s) 517, 1862, 2785
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 4 cut(s) 759, 974, 2433, 2593
Bsp1286I GDGCHC 2 cut(s) 2097, 2646
Bsp13I TCCGGA 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 259
Bsp143I GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
BspCNI CTCAG 9 cut(s) 201, 424, 1326, 1477, 1546, 1818, 2104, 2395, 2758
BspEI TCCGGA 1 cut(s) 622
BspFNI CGCG 1 cut(s) 2412
BspHI TCATGA 1 cut(s) 1923
BspMAI CTGCAG 1 cut(s) 1063
BspMI ACCTGC 4 cut(s) 549, 574, 1435, 1516
BspPI GGATC 4 cut(s) 821, 2005, 2286, 2700
BspT107I GGYRCC 1 cut(s) 2094
BspTNI GGTCTC 1 cut(s) 676
BsrDI GCAATG 4 cut(s) 393, 910, 1851, 2421
BsrFI RCCGGY 1 cut(s) 1300
BsrGI TGTACA 1 cut(s) 259
BsrI ACTGG 6 cut(s) 159, 1159, 1527, 1697, 2732, 2929
BssAI RCCGGY 1 cut(s) 1300
BssECI CCNNGG 9 cut(s) 69, 295, 439, 1396, 1686, 1743, 1836, 2593, 2838
BssMI GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
BssNI GRCGYC 2 cut(s) 1354, 1497
BssT1I CCWWGG 6 cut(s) 69, 295, 439, 1686, 1836, 2838
Bst2UI CCWGG 6 cut(s) 173, 462, 1398, 1744, 1884, 2879
Bst4CI ACNGT 9 cut(s) 604, 1459, 1585, 1723, 1972, 2183, 2272, 2513, 2657
BstACI GRCGYC 2 cut(s) 1354, 1497
BstAUI TGTACA 1 cut(s) 259
BstBAI YACGTR 2 cut(s) 1571, 1652
BstEII GGTNACC 1 cut(s) 2489
BstF5I GGATG 5 cut(s) 32, 181, 476, 2913, 2925
BstFNI CGCG 1 cut(s) 2412
BstH2I RGCGCY 1 cut(s) 2917
BstHHI GCGC 4 cut(s) 1522, 1921, 2414, 2916
BstKTI GATC 6 cut(s) 829, 1616, 2000, 2281, 2695, 2772
BstMAI GTCTC 4 cut(s) 416, 676, 1961, 2916
BstMBI GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
BstNI CCWGG 6 cut(s) 173, 462, 1398, 1744, 1884, 2879
BstNSI RCATGY 1 cut(s) 1848
BstPAI GACNNNNGTC 2 cut(s) 2613, 2750
BstPI GGTNACC 1 cut(s) 2489
BstSFI CTRYAG 1 cut(s) 1059
BstSLI GKGCMC 1 cut(s) 2097
BstUI CGCG 1 cut(s) 2412
BstV1I GCAGC 7 cut(s) 207, 390, 1396, 1860, 2583, 2636, 2956
BstV2I GAAGAC 3 cut(s) 437, 1061, 2855
BstX2I RGATCY 2 cut(s) 1997, 2692
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 2 cut(s) 1997, 2692
BsuI GTATCC 1 cut(s) 2755
BtsCI GGATG 5 cut(s) 32, 181, 476, 2913, 2925
BtsIMutI CAGTG 6 cut(s) 1152, 1581, 1728, 1813, 2662, 2739
BveI ACCTGC 4 cut(s) 549, 574, 1435, 1516
CaiI CAGNNNCTG 1 cut(s) 1508
CciI TCATGA 1 cut(s) 1923
CfoI GCGC 4 cut(s) 1522, 1921, 2414, 2916
Cfr10I RCCGGY 1 cut(s) 1300
Cfr9I CCCGGG 1 cut(s) 2593
CseI GACGC 1 cut(s) 1505
Csp6I GTAC 3 cut(s) 31, 260, 1003
CviAII CATG 8 cut(s) 79, 189, 1702, 1845, 1924, 2479, 2677, 2788
CviQI GTAC 3 cut(s) 31, 260, 1003
DpnI GATC 6 cut(s) 828, 1615, 1999, 2280, 2694, 2771
DpnII GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
DraI TTTAAA 1 cut(s) 1822
DrdI GACNNNNNNGTC 1 cut(s) 71
DriI GACNNNNNGTC 1 cut(s) 2929
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 169
Eam1105I GACNNNNNGTC 1 cut(s) 2929
Eco130I CCWWGG 6 cut(s) 69, 295, 439, 1686, 1836, 2838
Eco31I GGTCTC 1 cut(s) 676
Eco47I GGWCC 5 cut(s) 104, 365, 764, 1424, 1986
Eco57I CTGAAG 4 cut(s) 42, 351, 1073, 2777
Eco72I CACGTG 2 cut(s) 1571, 1652
Eco88I CYCGRG 4 cut(s) 759, 974, 2433, 2593
Eco91I GGTNACC 1 cut(s) 2489
EcoO109I RGGNCCY 5 cut(s) 436, 609, 664, 764, 1986
EcoO65I GGTNACC 1 cut(s) 2489
EcoRI GAATTC 2 cut(s) 278, 657
EcoRII CCWGG 6 cut(s) 171, 460, 1396, 1742, 1882, 2877
EcoT14I CCWWGG 6 cut(s) 69, 295, 439, 1686, 1836, 2838
ErhI CCWWGG 6 cut(s) 69, 295, 439, 1686, 1836, 2838
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 8 cut(s) 82, 192, 1705, 1848, 1927, 2482, 2680, 2791
FalI AAGNNNNNCTT 2 cut(s) 2034, 2066
FaqI GGGAC 8 cut(s) 77, 965, 1196, 1379, 1760, 1999, 2489, 2576
FatI CATG 8 cut(s) 78, 188, 1701, 1844, 1923, 2478, 2676, 2787
FauI CCCGC 2 cut(s) 1172, 2895
FauNDI CATATG 1 cut(s) 1309
FokI GGATG 5 cut(s) 39, 188, 463, 2912, 2920
FspBI CTAG 3 cut(s) 426, 1229, 2813
GlaI GCGC 4 cut(s) 1521, 1920, 2413, 2915
GsaI CCCAGC 2 cut(s) 340, 1505
GsuI CTGGAG 2 cut(s) 1419, 1680
HaeII RGCGCY 1 cut(s) 2917
HapII CCGG 8 cut(s) 102, 623, 1301, 1983, 2553, 2594, 2727, 2954
HgaI GACGC 1 cut(s) 1505
HhaI GCGC 4 cut(s) 1522, 1921, 2414, 2916
Hin1I GRCGYC 2 cut(s) 1354, 1497
Hin1II CATG 8 cut(s) 82, 192, 1705, 1848, 1927, 2482, 2680, 2791
Hin6I GCGC 4 cut(s) 1520, 1919, 2412, 2914
HinP1I GCGC 4 cut(s) 1520, 1919, 2412, 2914
HincII GTYRAC 1 cut(s) 2194
HindII GTYRAC 1 cut(s) 2194
HpaII CCGG 8 cut(s) 102, 623, 1301, 1983, 2553, 2594, 2727, 2954
HphI GGTGA 6 cut(s) 311, 482, 1502, 2090, 2242, 2378
Hpy166II GTNNAC 4 cut(s) 250, 368, 1226, 2194
Hpy8I GTNNAC 4 cut(s) 250, 368, 1226, 2194
HpyCH4III ACNGT 9 cut(s) 604, 1459, 1585, 1723, 1972, 2183, 2272, 2513, 2657
HpyCH4IV ACGT 9 cut(s) 409, 1005, 1025, 1354, 1570, 1651, 2583, 2830, 2946
HpySE526I ACGT 9 cut(s) 409, 1005, 1025, 1354, 1570, 1651, 2583, 2830, 2946
Hsp92I GRCGYC 2 cut(s) 1354, 1497
Hsp92II CATG 8 cut(s) 82, 192, 1705, 1848, 1927, 2482, 2680, 2791
HspAI GCGC 4 cut(s) 1520, 1919, 2412, 2914
Kpn2I TCCGGA 1 cut(s) 622
KroI GCCGGC 1 cut(s) 1300
KroNI GCCGGC 1 cut(s) 1302
Kzo9I GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
LmnI GCTCC 6 cut(s) 151, 1309, 1406, 1894, 2338, 2438
Lsp1109I GCAGC 7 cut(s) 207, 390, 1396, 1860, 2583, 2636, 2956
LweI GCATC 9 cut(s) 485, 517, 812, 897, 1478, 1618, 1736, 1822, 2321
MaeI CTAG 3 cut(s) 426, 1229, 2813
MaeII ACGT 9 cut(s) 409, 1005, 1025, 1354, 1570, 1651, 2583, 2830, 2946
MaeIII GTNAC 7 cut(s) 135, 299, 688, 2230, 2366, 2489, 2743
MalI GATC 6 cut(s) 828, 1615, 1999, 2280, 2694, 2771
MboI GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
MfeI CAATTG 1 cut(s) 1914
MflI RGATCY 2 cut(s) 1997, 2692
MhlI GDGCHC 2 cut(s) 2097, 2646
MlsI TGGCCA 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 6 cut(s) 59, 394, 544, 947, 2018, 2740
MmeI TCCRAC 1 cut(s) 1008
Mox20I TGGCCA 1 cut(s) 171
MroI TCCGGA 1 cut(s) 622
MroNI GCCGGC 1 cut(s) 1300
MroXI GAANNNNTTC 1 cut(s) 1678
MscI TGGCCA 1 cut(s) 171
MseI TTAA 6 cut(s) 1245, 1821, 2586, 2636, 2651, 2712
MslI CAYNNNNRTG 2 cut(s) 187, 879
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 1 cut(s) 2969
MspI CCGG 8 cut(s) 102, 623, 1301, 1983, 2553, 2594, 2727, 2954
MunI CAATTG 1 cut(s) 1914
Mva1269I GAATGC 3 cut(s) 517, 1862, 2785
MvaI CCWGG 6 cut(s) 173, 462, 1398, 1744, 1884, 2879
MvnI CGCG 1 cut(s) 2412
NaeI GCCGGC 1 cut(s) 1302
NciI CCSGG 5 cut(s) 1984, 2553, 2594, 2595, 2728
NdeI CATATG 1 cut(s) 1309
NdeII GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
NgoMIV GCCGGC 1 cut(s) 1300
NlaIII CATG 8 cut(s) 82, 192, 1705, 1848, 1927, 2482, 2680, 2791
NmeAIII GCCGAG 1 cut(s) 935
NmuCI GTSAC 5 cut(s) 135, 299, 2230, 2366, 2743
NspI RCATGY 1 cut(s) 1848
PaeR7I CTCGAG 3 cut(s) 759, 974, 2433
PagI TCATGA 1 cut(s) 1923
PaqCI CACCTGC 1 cut(s) 1516
PcsI WCGNNNNNNNCGW 1 cut(s) 2187
PctI GAATGC 3 cut(s) 517, 1862, 2785
PdiI GCCGGC 1 cut(s) 1302
PdmI GAANNNNTTC 1 cut(s) 1678
PfeI GAWTC 8 cut(s) 307, 487, 1205, 1433, 1831, 2117, 2320, 2447
PflFI GACNNNGTC 1 cut(s) 2372
PflMI CCANNNNNTGG 1 cut(s) 27
PfoI TCCNGGA 1 cut(s) 2877
PleI GAGTC 6 cut(s) 59, 393, 543, 947, 2017, 2740
PmaCI CACGTG 2 cut(s) 1571, 1652
PmlI CACGTG 2 cut(s) 1571, 1652
PpsI GAGTC 6 cut(s) 59, 393, 543, 947, 2017, 2740
Ppu21I YACGTR 2 cut(s) 1571, 1652
PpuMI RGGWCCY 2 cut(s) 764, 1986
PshAI GACNNNNGTC 2 cut(s) 2613, 2750
PsiI TTATAA 1 cut(s) 2352
Psp5II RGGWCCY 2 cut(s) 764, 1986
Psp6I CCWGG 6 cut(s) 171, 460, 1396, 1742, 1882, 2877
PspCI CACGTG 2 cut(s) 1571, 1652
PspEI GGTNACC 1 cut(s) 2489
PspFI CCCAGC 2 cut(s) 336, 1501
PspGI CCWGG 6 cut(s) 171, 460, 1396, 1742, 1882, 2877
PspPPI RGGWCCY 2 cut(s) 764, 1986
PspXI VCTCGAGB 3 cut(s) 759, 974, 2433
PstI CTGCAG 1 cut(s) 1063
PstNI CAGNNNCTG 1 cut(s) 1508
PsuI RGATCY 2 cut(s) 1997, 2692
PsyI GACNNNGTC 1 cut(s) 2372
RsaI GTAC 3 cut(s) 32, 261, 1004
RsaNI GTAC 3 cut(s) 31, 260, 1003
RseI CAYNNNNRTG 2 cut(s) 187, 879
SaqAI TTAA 6 cut(s) 1245, 1821, 2586, 2636, 2651, 2712
Sau3AI GATC 6 cut(s) 826, 1613, 1997, 2278, 2692, 2769
SchI GAGTC 6 cut(s) 59, 394, 544, 947, 2018, 2740
SduI GDGCHC 2 cut(s) 2097, 2646
SfaNI GCATC 9 cut(s) 485, 517, 812, 897, 1478, 1618, 1736, 1822, 2321
SfcI CTRYAG 1 cut(s) 1059
Sfr274I CTCGAG 3 cut(s) 759, 974, 2433
SinI GGWCC 5 cut(s) 104, 365, 764, 1424, 1986
SlaI CTCGAG 3 cut(s) 759, 974, 2433
SmaI CCCGGG 1 cut(s) 2595
SmiMI CAYNNNNRTG 2 cut(s) 187, 879
SmlI CTYRAG 3 cut(s) 759, 974, 2433
SmoI CTYRAG 3 cut(s) 759, 974, 2433
SspMI CTAG 3 cut(s) 426, 1229, 2813
StyI CCWWGG 6 cut(s) 69, 295, 439, 1686, 1836, 2838
TaaI ACNGT 9 cut(s) 604, 1459, 1585, 1723, 1972, 2183, 2272, 2513, 2657
TaiI ACGT 9 cut(s) 412, 1008, 1028, 1357, 1573, 1654, 2586, 2833, 2949
TaqI TCGA 7 cut(s) 579, 760, 975, 1597, 1965, 2434, 2859
TatI WGTACW 1 cut(s) 259
TauI GCSGC 4 cut(s) 1048, 1051, 2786, 2969
TfiI GAWTC 8 cut(s) 307, 487, 1205, 1433, 1831, 2117, 2320, 2447
Tru1I TTAA 6 cut(s) 1245, 1821, 2586, 2636, 2651, 2712
Tru9I TTAA 6 cut(s) 1245, 1821, 2586, 2636, 2651, 2712
TscAI CASTG 6 cut(s) 1159, 1588, 1728, 1813, 2662, 2739
TseFI GTSAC 5 cut(s) 135, 299, 2230, 2366, 2743
TseI GCWGC 7 cut(s) 195, 403, 1409, 1848, 2571, 2624, 2969
Tsp45I GTSAC 5 cut(s) 135, 299, 2230, 2366, 2743
TspDTI ATGAA 5 cut(s) 192, 437, 1127, 1503, 2837
TspGWI ACGGA 1 cut(s) 2934
TspMI CCCGGG 1 cut(s) 2593
TspRI CASTG 6 cut(s) 1159, 1588, 1728, 1813, 2662, 2739
Tth111I GACNNNGTC 1 cut(s) 2372
Van91I CCANNNNNTGG 1 cut(s) 27
VpaK11BI GGWCC 5 cut(s) 104, 365, 764, 1424, 1986
XceI RCATGY 1 cut(s) 1848
XcmI CCANNNNNNNNNTGG 1 cut(s) 1151
XhoI CTCGAG 3 cut(s) 759, 974, 2433
XmaI CCCGGG 1 cut(s) 2593
XmnI GAANNNNTTC 1 cut(s) 1678
XspI CTAG 3 cut(s) 426, 1229, 2813
ZraI GACGTC 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.