Rorug06G0272500

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004359
Physical Location & Seq
Forward (+)
42010640 .. 42013933
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0272500.1

Sequence Viewer

Length: 1005 bp
ATGTCACCGAGTGAGTTCACTTCATCGAATTTGATGGAATTAACCATGGGAAGTTCAAGTCAGAAACGCTCGAGATCAAGCTTTGGACGCAGTGTCAATCTTGCTCCACTTCCTGGATCGGTTGACAACAAAACTGCTGTTCAAGTTTCTACCATTGCAAAACCTGATTGTATGCGCCCCTTGCTTTTCACTGATTTGAATCAACAACCAGTGAAGCAATTTACTGCTACAAAATATTCCCCTGTGAATACCAGCATGTTGAGGGATCCGGCTGCTGCTGAGAGGGTTTTGCTTGACCTGCTTGGCCCTGTTGATGCCAGCATTATGCAGGCGTATACTGACAGTGATCTAAGGCAGAACTTAGCCCACCATGCCCTTGCTTCCTCCCTCCACTTAGCCATGTGGCTGAGGAAAACTGGAGATGTTTCGAGGCAGCGCGATGAAGCCATAGCTCGTGTTGCAGAGCTGGAGGAAAGAGTTAGAAGGTTTGAAAAGCTCTTTGTGAAACAGGAGGCTGAAAATGAGATTCTGAGCCTAGAGGTCGACGAGTACTGTAACATGAAGGAGGAATATGGGTCTAAGATCAAGAGTTTAGCAAATGAGGTTAACCAGCAGAAGCAGGTGACTGAGGTCTTGGAGAGCAAGTTCAAGGATATGGAGGCCATGCACCAGGAGAAGGCTACTCGTCTGGAAAAGGCCTTGAAGCAGCTTAACGACGCCAACATGCTGCTTGCTGAGGTGAGTGAAGGTCTTGGTGTGGCCAGGGAACCAGGGGCCAGGGATCCTAGACATTTCTGGACGGTTTGCATTCACTGCAAAGTCCAGTATCAGTATATCAGGATCTATCAGAATCGCAGGCTTCGGTGCCCGAATTGTCGAAATGCTTTTCTTGCTATAGAAACTGTTTCATCTCCGAAGTCCTATCCATGGAGTAGCTCTTCTTGGGTGAAGCCTGAGGAAGATGGACAGGGTGCAGTCAAAGTGGAGGCTCAGGATGCCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

334

Amino Acids

37.7

Weight (kDa)

7.02

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 265 - 299 1.7e-14 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 610
Acc36I ACCTGC 2 cut(s) 306, 610
AccB1I GGYRCC 1 cut(s) 864
AccB7I CCANNNNNTGG 1 cut(s) 113
AccI GTMKAC 2 cut(s) 335, 543
AccII CGCG 1 cut(s) 438
AclWI GGATC 6 cut(s) 124, 260, 273, 776, 789, 848
AcoI YGGCCR 1 cut(s) 759
AcsI RAATTY 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 717
AdeI CACNNNGTG 1 cut(s) 11
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 3 cut(s) 113, 676, 927
AgsI TTSAA 6 cut(s) 57, 143, 199, 491, 649, 703
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 5 cut(s) 112, 669, 761, 769, 776
AloI GAACNNNNNNTCC 2 cut(s) 629, 661
AluBI AGCT 6 cut(s) 81, 452, 466, 496, 709, 936
AluI AGCT 6 cut(s) 81, 452, 466, 496, 709, 936
AlwI GGATC 6 cut(s) 124, 260, 273, 776, 789, 848
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 5 cut(s) 304, 660, 696, 759, 774
ApeKI GCWGC 5 cut(s) 272, 275, 433, 706, 727
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 2 cut(s) 177, 438
AspS9I GGNCC 2 cut(s) 305, 774
AsuHPI GGTGA 3 cut(s) 634, 751, 958
AvaI CYCGRG 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 954
BaeGI GKGCMC 1 cut(s) 869
BalI TGGCCA 1 cut(s) 761
BamHI GGATCC 2 cut(s) 265, 781
BanI GGYRCC 1 cut(s) 864
BauI CACGAG 1 cut(s) 453
BbvCI CCTCAGC 2 cut(s) 407, 735
BbvI GCAGC 4 cut(s) 151, 172, 877, 961
BccI CCATC 2 cut(s) 28, 956
BciT130I CCWGG 5 cut(s) 114, 671, 763, 771, 778
BfaI CTAG 2 cut(s) 536, 786
BfmI CTRYAG 1 cut(s) 894
BfuAI ACCTGC 2 cut(s) 306, 610
BisI GCNGC 5 cut(s) 273, 276, 434, 707, 728
BlsI GCNGC 5 cut(s) 274, 277, 435, 708, 729
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 5 cut(s) 114, 671, 763, 771, 778
BmeRI GACNNNNNGTC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 305, 774
BmiI GGNNCC 5 cut(s) 267, 768, 775, 783, 866
BmrFI CCNGG 5 cut(s) 114, 671, 763, 771, 778
BmsI GCATC 2 cut(s) 304, 985
BoxI GACNNNNGTC 1 cut(s) 629
BpmI CTGGAG 2 cut(s) 438, 488
Bpu10I CCTNAGC 3 cut(s) 407, 735, 990
BsaBI GATNNNNATC 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 5 cut(s) 45, 762, 770, 777, 926
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 676, 927
Bse1I ACTGG 3 cut(s) 209, 421, 823
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 5 cut(s) 114, 671, 763, 771, 778
BseDI CCNNGG 5 cut(s) 45, 762, 770, 777, 926
BseGI GGATG 1 cut(s) 1000
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 113, 676, 927
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 7 cut(s) 270, 398, 521, 618, 726, 945, 1004
BseNI ACTGG 3 cut(s) 209, 421, 823
BseSI GKGCMC 1 cut(s) 869
BseXI GCAGC 5 cut(s) 259, 262, 445, 714, 718
BsgI GTGCAG 1 cut(s) 993
Bsh1236I CGCG 1 cut(s) 438
BshFI GGCC 5 cut(s) 306, 662, 698, 761, 776
BshNI GGYRCC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 113, 676, 927
BsmI GAATGC 1 cut(s) 807
BsnI GGCC 5 cut(s) 306, 662, 698, 761, 776
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 869
Bsp143I GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
Bsp19I CCATGG 2 cut(s) 45, 926
BspANI GGCC 5 cut(s) 306, 662, 698, 761, 776
BspCNI CTCAG 7 cut(s) 271, 399, 522, 619, 727, 946, 1003
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 5 cut(s) 267, 768, 775, 783, 866
BspMI ACCTGC 2 cut(s) 306, 610
BspPI GGATC 6 cut(s) 124, 260, 273, 776, 789, 848
BspQI GCTCTTC 1 cut(s) 943
BspT107I GGYRCC 1 cut(s) 864
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 3 cut(s) 209, 421, 823
BssECI CCNNGG 5 cut(s) 45, 762, 770, 777, 926
BssMI GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
BssNAI GTATAC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 717
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 45, 926
Bst1107I GTATAC 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 5 cut(s) 114, 671, 763, 771, 778
Bst4CI ACNGT 4 cut(s) 344, 554, 802, 904
Bst6I CTCTTC 1 cut(s) 943
BstACI GRCGYC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 813
BstC8I GCNNGC 4 cut(s) 319, 330, 732, 857
BstDSI CCRYGG 2 cut(s) 45, 926
BstF5I GGATG 1 cut(s) 1000
BstFNI CGCG 1 cut(s) 438
BstHHI GCGC 2 cut(s) 177, 438
BstKTI GATC 7 cut(s) 77, 119, 268, 349, 585, 784, 843
BstMBI GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
BstMWI GCNNNNNNNGC 8 cut(s) 87, 181, 298, 371, 458, 813, 890, 995
BstNI CCWGG 5 cut(s) 114, 671, 763, 771, 778
BstNSI RCATGY 2 cut(s) 259, 727
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 112, 669, 761, 769, 776
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 1 cut(s) 869
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 5 cut(s) 259, 262, 445, 714, 718
BstX2I RGATCY 3 cut(s) 265, 781, 840
BstYI RGATCY 3 cut(s) 265, 781, 840
BstZ17I GTATAC 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 954
BsuRI GGCC 5 cut(s) 306, 662, 698, 761, 776
BtgI CCRYGG 2 cut(s) 45, 926
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 1000
BtsI GCAGTG 2 cut(s) 97, 811
BtsIMutI CAGTG 5 cut(s) 97, 189, 216, 349, 811
BveI ACCTGC 2 cut(s) 306, 610
Cac8I GCNNGC 4 cut(s) 319, 330, 732, 857
CfoI GCGC 2 cut(s) 177, 438
Cfr13I GGNCC 2 cut(s) 305, 774
CseI GACGC 2 cut(s) 96, 725
Csp6I GTAC 1 cut(s) 550
CviAII CATG 8 cut(s) 46, 256, 371, 400, 559, 664, 724, 927
CviQI GTAC 1 cut(s) 550
DpnI GATC 7 cut(s) 76, 118, 267, 348, 584, 783, 842
DpnII GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
DraIII CACNNNGTG 1 cut(s) 11
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 943
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 943
Eco130I CCWWGG 2 cut(s) 45, 926
Eco147I AGGCCT 1 cut(s) 698
Eco81I CCTNAGG 1 cut(s) 954
Eco88I CYCGRG 1 cut(s) 70
EcoRII CCWGG 5 cut(s) 112, 669, 761, 769, 776
EcoT14I CCWWGG 2 cut(s) 45, 926
ErhI CCWWGG 2 cut(s) 45, 926
FaeI CATG 8 cut(s) 49, 259, 374, 403, 562, 667, 727, 930
FatI CATG 8 cut(s) 45, 255, 370, 399, 558, 663, 723, 926
FblI GTMKAC 2 cut(s) 335, 543
Fnu4HI GCNGC 5 cut(s) 273, 276, 434, 707, 728
Fsp4HI GCNGC 5 cut(s) 273, 276, 434, 707, 728
FspBI CTAG 2 cut(s) 536, 786
GlaI GCGC 2 cut(s) 176, 437
GluI GCNGC 5 cut(s) 273, 276, 434, 707, 728
GsuI CTGGAG 2 cut(s) 438, 488
HaeIII GGCC 5 cut(s) 306, 662, 698, 761, 776
HapII CCGG 1 cut(s) 269
HgaI GACGC 2 cut(s) 96, 725
HhaI GCGC 2 cut(s) 177, 438
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 8 cut(s) 49, 259, 374, 403, 562, 667, 727, 930
Hin6I GCGC 2 cut(s) 175, 436
HinP1I GCGC 2 cut(s) 175, 436
HincII GTYRAC 3 cut(s) 124, 544, 607
HindII GTYRAC 3 cut(s) 124, 544, 607
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 3 cut(s) 199, 526, 850
HpaI GTTAAC 1 cut(s) 607
HpaII CCGG 1 cut(s) 269
HphI GGTGA 3 cut(s) 634, 751, 958
Hpy166II GTNNAC 5 cut(s) 18, 124, 336, 544, 607
Hpy188I TCNGA 4 cut(s) 63, 531, 849, 915
Hpy188III TCNNGA 6 cut(s) 72, 586, 689, 796, 838, 992
Hpy8I GTNNAC 5 cut(s) 18, 124, 336, 544, 607
Hpy99I CGWCG 2 cut(s) 548, 719
HpyAV CCTTC 4 cut(s) 477, 556, 670, 740
HpyCH4III ACNGT 4 cut(s) 344, 554, 802, 904
HpyCH4V TGCA 7 cut(s) 158, 328, 461, 667, 807, 816, 974
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 181, 298, 371, 458, 813, 890, 995
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 8 cut(s) 49, 259, 374, 403, 562, 667, 727, 930
HspAI GCGC 2 cut(s) 175, 436
KspAI GTTAAC 1 cut(s) 607
Kzo9I GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
LguI GCTCTTC 1 cut(s) 943
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 5 cut(s) 259, 262, 445, 714, 718
LweI GCATC 2 cut(s) 304, 985
MaeI CTAG 2 cut(s) 536, 786
MaeIII GTNAC 3 cut(s) 3, 554, 622
MalI GATC 7 cut(s) 76, 118, 267, 348, 584, 783, 842
MboI GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
MboII GAAGA 2 cut(s) 930, 971
MflI RGATCY 3 cut(s) 265, 781, 840
MhlI GDGCHC 1 cut(s) 869
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 4 cut(s) 28, 38, 218, 871
MluNI TGGCCA 1 cut(s) 761
Mox20I TGGCCA 1 cut(s) 761
MscI TGGCCA 1 cut(s) 761
MseI TTAA 3 cut(s) 41, 606, 711
Msp20I TGGCCA 1 cut(s) 761
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 5 cut(s) 114, 671, 763, 771, 778
Mva1269I GAATGC 1 cut(s) 807
MvaI CCWGG 5 cut(s) 114, 671, 763, 771, 778
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 8 cut(s) 87, 181, 298, 371, 458, 813, 890, 995
NcoI CCATGG 2 cut(s) 45, 926
NdeII GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
NlaIII CATG 8 cut(s) 49, 259, 374, 403, 562, 667, 727, 930
NlaIV GGNNCC 5 cut(s) 267, 768, 775, 783, 866
NmuCI GTSAC 2 cut(s) 3, 622
NspI RCATGY 2 cut(s) 259, 727
PaeR7I CTCGAG 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 610
PceI AGGCCT 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 943
PctI GAATGC 1 cut(s) 807
PfeI GAWTC 3 cut(s) 199, 526, 850
PflMI CCANNNNNTGG 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 5 cut(s) 274, 277, 435, 708, 729
PshAI GACNNNNGTC 1 cut(s) 629
Psp6I CCWGG 5 cut(s) 112, 669, 761, 769, 776
PspGI CCWGG 5 cut(s) 112, 669, 761, 769, 776
PspN4I GGNNCC 5 cut(s) 267, 768, 775, 783, 866
PspPI GGNCC 2 cut(s) 305, 774
PsuI RGATCY 3 cut(s) 265, 781, 840
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
SalI GTCGAC 1 cut(s) 542
SapI GCTCTTC 1 cut(s) 943
SaqAI TTAA 3 cut(s) 41, 606, 711
SatI GCNGC 5 cut(s) 273, 276, 434, 707, 728
Sau3AI GATC 7 cut(s) 74, 116, 265, 346, 582, 781, 840
Sau96I GGNCC 2 cut(s) 305, 774
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 5 cut(s) 114, 671, 763, 771, 778
SduI GDGCHC 1 cut(s) 869
SfaNI GCATC 2 cut(s) 304, 985
SfcI CTRYAG 1 cut(s) 894
Sfr274I CTCGAG 1 cut(s) 70
SlaI CTCGAG 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
Sse9I AATT 4 cut(s) 28, 38, 218, 871
SseBI AGGCCT 1 cut(s) 698
SspI AATATT 1 cut(s) 236
SspMI CTAG 2 cut(s) 536, 786
StuI AGGCCT 1 cut(s) 698
StyD4I CCNGG 5 cut(s) 112, 669, 761, 769, 776
StyI CCWWGG 2 cut(s) 45, 926
TaaI ACNGT 4 cut(s) 344, 554, 802, 904
TaqI TCGA 5 cut(s) 26, 71, 428, 543, 877
TasI AATT 4 cut(s) 28, 38, 218, 871
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 3 cut(s) 199, 526, 850
Tru1I TTAA 3 cut(s) 41, 606, 711
Tru9I TTAA 3 cut(s) 41, 606, 711
TscAI CASTG 5 cut(s) 97, 196, 216, 349, 818
TseFI GTSAC 2 cut(s) 3, 622
TseI GCWGC 5 cut(s) 272, 275, 433, 706, 727
Tsp45I GTSAC 2 cut(s) 3, 622
TspDTI ATGAA 4 cut(s) 12, 456, 575, 897
TspRI CASTG 5 cut(s) 97, 196, 216, 349, 818
Van91I CCANNNNNTGG 1 cut(s) 113
XapI RAATTY 1 cut(s) 28
XceI RCATGY 2 cut(s) 259, 727
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 2 cut(s) 335, 543
XspI CTAG 2 cut(s) 536, 786
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.