Rorug06G0489900

Staphylococcal nuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004359
Physical Location & Seq
Reverse (-)
60822125 .. 60824087
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0489900.1

Sequence Viewer

Length: 657 bp
ATGGAATTCGAGTGTGCTTCTCTCAGTCTCTGGAAGGAAGCCCTCTCTTCCTATTCATCTCGCATAGAATCTCTCAACAAACCCGATTTGATTTCCCTGGACGACTTTTACCGCAACCAGCTCCCTCCTCTCCTCCGCCACCGGAACCCTAACCCCCACCTCACCACCCCCGAGCTCTCCGATCTCATGCGCTGGAAGCTCACACGTGGCAAGTGGCGGCCGAGGCTGTTGGATTTCGTTTCGGCATTGGACGAATCGGTGGTGAAATTGGCTTCAGAGAAGGCGTTTCGGTCGCTTCCTGATCTCTCAAAGGCGGTCTCTGAGCTCACTGTGCTGAAAGGAGTGGGACCGGCCACGGCCTCGGCTGTTCTGGCTGCTTATGCGCCGGATGTGGCGCCTTTCATGTCCGACGAGGCTATGGAGGCAGCTCTTGGTAATAGCAAGGACTATTCACTTAAGCAGTATCTGTTGTTTGCAGATAAGTTGCAGAAAAAAGCAAAGGAATTGAGTGCAGAAGGGGAAACCTTCACACCATCAGATGTAGAAAGGGCTCTATGGAGTTGTGCTGTGGGGGCCAAATTGCAATCTTCACAGTCAGATCCAGACCCCAAAGTCACTGATAAAACTAAAAACCCCAAGAGAAAGAGAAAGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000932 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010371 GO:0010372 GO:0010467 GO:0010468 GO:0010494 GO:0010565 GO:0010605 GO:0010629 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016070 GO:0016071 GO:0016101 GO:0016102 GO:0016114 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019439 GO:0019747 GO:0019752 GO:0030054 GO:0030312 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043455 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046394 GO:0046483 GO:0046686 GO:0046700 GO:0046885 GO:0046886 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090305 GO:0090351 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05169 ko05203 map05169 map05203
Pfam Domains
Protein Families
SNase TUDOR

Protein Analysis

218

Amino Acids

24.36

Weight (kDa)

9.1

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 394
AciI CCGC 4 cut(s) 112, 136, 217, 314
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 2 cut(s) 218, 351
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 1 cut(s) 258
AcvI CACGTG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 395
AfeI AGCGCT 1 cut(s) 653
AflII CTTAAG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 203
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 5 cut(s) 121, 175, 199, 325, 428
AluI AGCT 5 cut(s) 121, 175, 199, 325, 428
Alw21I GWGCWC 2 cut(s) 177, 327
Alw26I GTCTC 2 cut(s) 32, 322
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 2 cut(s) 30, 466
Ama87I CYCGRG 1 cut(s) 170
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 4 cut(s) 218, 351, 357, 573
ApeKI GCWGC 2 cut(s) 374, 425
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 4 cut(s) 192, 385, 397, 654
AspS9I GGNCC 2 cut(s) 347, 573
AsuHPI GGTGA 2 cut(s) 154, 274
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 347
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 177, 327, 553
BbrPI CACGTG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 177, 327
BbvI GCAGC 2 cut(s) 361, 437
BccI CCATC 1 cut(s) 541
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 32, 322
BfoI RGCGCY 2 cut(s) 398, 655
BfrI CTTAAG 1 cut(s) 455
BisI GCNGC 3 cut(s) 218, 375, 426
BlsI GCNGC 3 cut(s) 219, 376, 427
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 347, 573
BmiI GGNNCC 4 cut(s) 146, 348, 396, 574
BmrFI CCNGG 1 cut(s) 98
BsaAI YACGTR 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 4 cut(s) 96, 221, 354, 360
BsaWI WCCGGW 1 cut(s) 141
Bse118I RCCGGY 1 cut(s) 349
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 4 cut(s) 96, 221, 354, 360
BseGI GGATG 1 cut(s) 394
BseMII CTCAG 2 cut(s) 37, 312
BseRI GAGGAG 2 cut(s) 117, 122
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 2 cut(s) 361, 437
BsgI GTGCAG 1 cut(s) 531
Bsh1285I CGRYCG 2 cut(s) 221, 293
BshFI GGCC 4 cut(s) 220, 353, 359, 575
BshNI GGYRCC 1 cut(s) 394
BsiEI CGRYCG 2 cut(s) 221, 293
BsiHKAI GWGCWC 2 cut(s) 177, 327
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 3 cut(s) 142, 350, 386
BslFI GGGAC 1 cut(s) 360
BsmAI GTCTC 2 cut(s) 32, 322
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 4 cut(s) 220, 353, 359, 575
Bso31I GGTCTC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 177, 327, 553
Bsp143I GATC 3 cut(s) 181, 301, 598
BspACI CCGC 4 cut(s) 112, 136, 217, 314
BspANI GGCC 4 cut(s) 220, 353, 359, 575
BspCNI CTCAG 2 cut(s) 36, 313
BspLI GGNNCC 4 cut(s) 146, 348, 396, 574
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 394
BspTI CTTAAG 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 4 cut(s) 96, 221, 354, 360
BssMI GATC 3 cut(s) 181, 301, 598
BssNI GRCGYC 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 331, 594
Bst6I CTCTTC 1 cut(s) 52
BstACI GRCGYC 1 cut(s) 395
BstAFI CTTAAG 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 206
BstDEI CTNAG 2 cut(s) 23, 321
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 2 cut(s) 398, 655
BstHHI GCGC 4 cut(s) 192, 385, 397, 654
BstKTI GATC 3 cut(s) 184, 304, 601
BstMAI GTCTC 2 cut(s) 32, 322
BstMBI GATC 3 cut(s) 181, 301, 598
BstMCI CGRYCG 2 cut(s) 221, 293
BstMWI GCNNNNNNNGC 7 cut(s) 196, 223, 331, 371, 380, 422, 572
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 361, 437
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 4 cut(s) 220, 353, 359, 575
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 327, 615
CaiI CAGNNNCTG 2 cut(s) 30, 466
CfoI GCGC 4 cut(s) 192, 385, 397, 654
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 347, 573
CviAII CATG 2 cut(s) 187, 403
DdeI CTNAG 2 cut(s) 23, 321
DinI GGCGCC 1 cut(s) 396
DpnI GATC 3 cut(s) 183, 303, 600
DpnII GATC 3 cut(s) 181, 301, 598
DrdI GACNNNNNNGTC 1 cut(s) 611
DseDI GACNNNNNNGTC 1 cut(s) 611
EaeI YGGCCR 2 cut(s) 218, 351
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 125
Ecl136II GAGCTC 2 cut(s) 175, 325
EclXI CGGCCG 1 cut(s) 218
Eco24I GRGCYC 3 cut(s) 177, 327, 553
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 347
Eco47III AGCGCT 1 cut(s) 653
Eco52I CGGCCG 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 175, 325
Eco57I CTGAAG 1 cut(s) 258
Eco72I CACGTG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 2 cut(s) 175, 325
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 3 cut(s) 177, 327, 553
EgeI GGCGCC 1 cut(s) 396
EheI GGCGCC 1 cut(s) 396
FaeI CATG 2 cut(s) 190, 406
FaiI YATR 6 cut(s) 65, 188, 381, 404, 419, 556
FaqI GGGAC 1 cut(s) 360
FatI CATG 2 cut(s) 186, 402
Fnu4HI GCNGC 3 cut(s) 218, 375, 426
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 3 cut(s) 177, 327, 553
Fsp4HI GCNGC 3 cut(s) 218, 375, 426
GlaI GCGC 4 cut(s) 191, 384, 396, 653
GluI GCNGC 3 cut(s) 218, 375, 426
HaeII RGCGCY 2 cut(s) 398, 655
HaeIII GGCC 4 cut(s) 220, 353, 359, 575
HapII CCGG 3 cut(s) 142, 350, 386
HhaI GCGC 4 cut(s) 192, 385, 397, 654
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 2 cut(s) 190, 406
Hin6I GCGC 4 cut(s) 190, 383, 395, 652
HinP1I GCGC 4 cut(s) 190, 383, 395, 652
HinfI GANTC 2 cut(s) 68, 254
HpaII CCGG 3 cut(s) 142, 350, 386
HphI GGTGA 2 cut(s) 154, 274
Hpy188I TCNGA 6 cut(s) 181, 277, 322, 409, 538, 598
Hpy188III TCNNGA 3 cut(s) 31, 299, 602
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 4 cut(s) 28, 274, 509, 535
HpyCH4III ACNGT 2 cut(s) 331, 594
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 476, 487, 512, 583
HpyF10VI GCNNNNNNNGC 7 cut(s) 196, 223, 331, 371, 380, 422, 572
HpyF3I CTNAG 2 cut(s) 23, 321
HpySE526I ACGT 1 cut(s) 205
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 2 cut(s) 190, 406
HspAI GCGC 4 cut(s) 190, 383, 395, 652
KasI GGCGCC 1 cut(s) 394
Kzo9I GATC 3 cut(s) 181, 301, 598
LmnI GCTCC 1 cut(s) 126
Lsp1109I GCAGC 2 cut(s) 361, 437
MaeII ACGT 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 613
MalI GATC 3 cut(s) 183, 303, 600
MboI GATC 3 cut(s) 181, 301, 598
MboII GAAGA 2 cut(s) 39, 579
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 3 cut(s) 177, 327, 553
MluCI AATT 4 cut(s) 5, 266, 503, 578
Mly113I GGCGCC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 210, 432
MnlI CCTC 9 cut(s) 53, 135, 138, 143, 170, 216, 370, 406, 415
MseI TTAA 1 cut(s) 456
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 3 cut(s) 142, 350, 386
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 7 cut(s) 196, 223, 331, 371, 380, 422, 572
NarI GGCGCC 1 cut(s) 395
NdeII GATC 3 cut(s) 181, 301, 598
NlaIII CATG 2 cut(s) 190, 406
NlaIV GGNNCC 4 cut(s) 146, 348, 396, 574
NmeAIII GCCGAG 2 cut(s) 246, 341
NmuCI GTSAC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 68, 254
PkrI GCNGC 3 cut(s) 219, 376, 427
PluTI GGCGCC 1 cut(s) 398
PmaCI CACGTG 1 cut(s) 206
PmlI CACGTG 1 cut(s) 206
Ppu21I YACGTR 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 177, 327
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 4 cut(s) 146, 348, 396, 574
PspPI GGNCC 2 cut(s) 347, 573
PstNI CAGNNNCTG 2 cut(s) 30, 466
PsuI RGATCY 1 cut(s) 598
SacI GAGCTC 2 cut(s) 177, 327
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 3 cut(s) 218, 375, 426
Sau3AI GATC 3 cut(s) 181, 301, 598
Sau96I GGNCC 2 cut(s) 347, 573
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 3 cut(s) 177, 327, 553
SetI ASST 8 cut(s) 123, 162, 177, 201, 208, 327, 430, 527
SfoI GGCGCC 1 cut(s) 396
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 4 cut(s) 5, 266, 503, 578
SsiI CCGC 4 cut(s) 112, 136, 217, 314
SspDI GGCGCC 1 cut(s) 394
SstI GAGCTC 2 cut(s) 177, 327
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 331, 594
TaiI ACGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 9
TaqII GACCGA 1 cut(s) 279
TasI AATT 4 cut(s) 5, 266, 503, 578
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 2 cut(s) 68, 254
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 2 cut(s) 334, 622
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 374, 425
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 45, 391
TspRI CASTG 2 cut(s) 334, 622
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.