MD14G1223300.v1.1

ATP-dependent Clp protease adapter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004724
Physical Location & Seq
Forward (+)
30453281 .. 30455452
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1223300.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTGCCATCTGCGGTCGAGTCCCTCTTTCGCCAAACCATGGATTCAATCTCAACAAATCAGGTGATAAACATTATTTCCACAGACAAAGTCCAAATCGGAGCATCTTGATGACAGCATCGAGCACCAGGCTTGGCACAGGTGGTGGGGTGTTGGACAAGCCAACCATAGAGAAAACAACTCCTGGTCGTGACTCTGAATTTGACGTGAGGAAATCAAGGAAAATGTCTCCACCATACCGAGTGATGCTGCACAATGACAACTATAACAAGAGGGAGTACGTTGTCCAAGTTCTGATGAAAGTCATCCCAGGAATGACAGTCGACATTGCGGTGAACGTGATGCAGGAGGCGCATCACAACGGCCTGGCTGTCGTAATTGTGTGTGCTCAGGTGGATGCAGAAGATCACTGCATGCAGCTCAGAGGCAACGGGCTTTTAAGCTCCGTTGAGCCCGCCAGTGATGGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ClpS

Protein Analysis

158

Amino Acids

17.23

Weight (kDa)

8.73

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpS PF02617 77 - 143 1.3e-17 ATP-dependent Clp protease adaptor protein ClpS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 41
AccI GTMKAC 1 cut(s) 324
AciI CCGC 3 cut(s) 15, 332, 456
AcsI RAATTY 1 cut(s) 200
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 1 cut(s) 49
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 4 cut(s) 128, 184, 310, 366
AluBI AGCT 2 cut(s) 421, 444
AluI AGCT 2 cut(s) 421, 444
Alw21I GWGCWC 2 cut(s) 128, 391
Alw26I GTCTC 1 cut(s) 234
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 5, 250, 418
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 77, 346
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 2 cut(s) 128, 391
BbvI GCAGC 2 cut(s) 237, 430
BccI CCATC 2 cut(s) 17, 458
BceAI ACGGC 1 cut(s) 379
BciT130I CCWGG 4 cut(s) 130, 186, 312, 368
BcoDI GTCTC 1 cut(s) 234
BisI GCNGC 3 cut(s) 6, 251, 419
BlsI GCNGC 3 cut(s) 7, 252, 420
Bme1390I CCNGG 4 cut(s) 130, 186, 312, 368
BmgBI CACGTC 1 cut(s) 208
BmrFI CCNGG 4 cut(s) 130, 186, 312, 368
BmsI GCATC 6 cut(s) 114, 128, 237, 333, 364, 388
Bpu10I CCTNAGC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 40, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 459
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 4 cut(s) 130, 186, 312, 368
BseDI CCNNGG 2 cut(s) 40, 310
BseGI GGATG 3 cut(s) 306, 403, 473
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 404, 436
BseNI ACTGG 1 cut(s) 459
BseXI GCAGC 2 cut(s) 237, 430
BsgI GTGCAG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 128, 391
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 1 cut(s) 366
Bsp1286I GDGCHC 3 cut(s) 128, 391, 456
Bsp143I GATC 1 cut(s) 406
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 3 cut(s) 15, 332, 456
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 2 cut(s) 403, 435
BsrDI GCAATG 1 cut(s) 327
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 2 cut(s) 40, 310
BssMI GATC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 4 cut(s) 130, 186, 312, 368
Bst4CI ACNGT 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 416, 456
BstDEI CTNAG 2 cut(s) 390, 422
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 306, 403, 473
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 406
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNI CCWGG 4 cut(s) 130, 186, 312, 368
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 128, 184, 310, 366
BstV1I GCAGC 2 cut(s) 237, 430
BsuRI GGCC 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 40
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 3 cut(s) 306, 403, 473
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 409, 466
Cac8I GCNNGC 2 cut(s) 416, 456
CfoI GCGC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 41, 415
CviJI RGCY 9 cut(s) 5, 133, 163, 366, 371, 421, 436, 444, 454
CviKI_1 RGCY 9 cut(s) 5, 133, 163, 366, 371, 421, 436, 444, 454
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 390, 422
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 456
EcoRII CCWGG 4 cut(s) 128, 184, 310, 366
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 44, 418
FaiI YATR 5 cut(s) 42, 170, 238, 267, 416
FaqI GGGAC 1 cut(s) 8
FatI CATG 2 cut(s) 40, 414
FauI CCCGC 1 cut(s) 463
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 3 cut(s) 6, 251, 419
FokI GGATG 2 cut(s) 293, 410
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 6, 251, 419
GlaI GCGC 1 cut(s) 354
GluI GCNGC 3 cut(s) 6, 251, 419
HaeIII GGCC 1 cut(s) 366
HhaI GCGC 1 cut(s) 355
Hin1II CATG 2 cut(s) 44, 418
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 3 cut(s) 21, 45, 194
HphI GGTGA 2 cut(s) 77, 346
Hpy166II GTNNAC 2 cut(s) 325, 337
Hpy188I TCNGA 4 cut(s) 102, 199, 297, 425
Hpy188III TCNNGA 2 cut(s) 109, 191
Hpy8I GTNNAC 2 cut(s) 325, 337
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 3 cut(s) 207, 282, 339
HpyCH4V TGCA 5 cut(s) 253, 346, 401, 414, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 2 cut(s) 390, 422
HpySE526I ACGT 3 cut(s) 207, 282, 339
Hsp92II CATG 2 cut(s) 44, 418
HspAI GCGC 1 cut(s) 353
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 102, 449
Lsp1109I GCAGC 2 cut(s) 237, 430
LweI GCATC 6 cut(s) 114, 128, 237, 333, 364, 388
MaeII ACGT 3 cut(s) 207, 282, 339
MaeIII GTNAC 1 cut(s) 191
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 1 cut(s) 416
MhlI GDGCHC 3 cut(s) 128, 391, 456
MluCI AATT 2 cut(s) 200, 378
MlyI GAGTC 2 cut(s) 30, 188
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 5 cut(s) 36, 204, 267, 343, 419
MseI TTAA 1 cut(s) 440
MslI CAYNNNNRTG 2 cut(s) 110, 332
MspR9I CCNGG 4 cut(s) 130, 186, 312, 368
MvaI CCWGG 4 cut(s) 130, 186, 312, 368
MwoI GCNNNNNNNGC 1 cut(s) 352
NcoI CCATGG 1 cut(s) 40
NdeII GATC 1 cut(s) 406
NlaIII CATG 2 cut(s) 44, 418
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 418
PaeI GCATGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 45
PflFI GACNNNGTC 1 cut(s) 90
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 3 cut(s) 7, 252, 420
PleI GAGTC 2 cut(s) 29, 188
PpsI GAGTC 2 cut(s) 29, 188
Psp6I CCWGG 4 cut(s) 128, 184, 310, 366
PspGI CCWGG 4 cut(s) 128, 184, 310, 366
PsyI GACNNNGTC 1 cut(s) 90
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 2 cut(s) 110, 332
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 3 cut(s) 6, 251, 419
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 2 cut(s) 30, 188
ScrFI CCNGG 4 cut(s) 130, 186, 312, 368
SduI GDGCHC 3 cut(s) 128, 391, 456
SetI ASST 8 cut(s) 67, 145, 210, 285, 342, 396, 423, 446
SfaNI GCATC 6 cut(s) 114, 128, 237, 333, 364, 388
SmiMI CAYNNNNRTG 2 cut(s) 110, 332
SphI GCATGC 1 cut(s) 418
Sse9I AATT 2 cut(s) 200, 378
SsiI CCGC 3 cut(s) 15, 332, 456
StyD4I CCNGG 4 cut(s) 128, 184, 310, 366
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 3 cut(s) 210, 285, 342
TaqI TCGA 3 cut(s) 19, 122, 324
TasI AATT 2 cut(s) 200, 378
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 2 cut(s) 416, 466
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 5, 250, 418
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 2 cut(s) 416, 466
Tth111I GACNNNGTC 1 cut(s) 90
Van91I CCANNNNNTGG 1 cut(s) 41
XapI RAATTY 1 cut(s) 200
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.