MD16G1046400.v1.1

ATP-dependent Clp protease adapter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004724
Physical Location & Seq
Reverse (-)
3229375 .. 3231742
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1046400.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGAGACTGCGATTTGCGGGAGAATCCCTCTCTCCCCCAACCACGTCTTCAACCCCAAACCAGGAGACAGACACCCTCTTTGTAAAGGAAAATGCACCAATATTAGCATTCCTGTGGCTGTATCAGCAGCAGTACCTGGTAAAGGAGGAGGGTTATTGGAAAGGCCAGTTAGAGAGAAAACAACTCCTGCCCGTGATTCTGAGTTTGATTTGAGGAAATCAAGGAAGCCAGCTCCTCCATATCGTGTAATACTGCACAACGACAACTTCAACAAACGGGAATATGTTGTGCAAGTACTGATGAAGGTTATTCCGGGGATGACCCTTGACAATGCTGTTAATATTATGCAAGAAGCACACCACAATGGCTTGTCGGTAGTGATCATATGTGCACAGGCCGATGCTGAAGAACACTGCATGCAACTGAGACACAATGGACTTCTGAGTTCAATTGAACCTGCAGGTGGCGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ClpS

Protein Analysis

159

Amino Acids

17.18

Weight (kDa)

8.81

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpS PF02617 77 - 144 4.6e-19 ATP-dependent Clp protease adaptor protein ClpS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 2 cut(s) 452, 466
AciI CCGC 2 cut(s) 18, 468
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 2 cut(s) 135, 297
AfiI CCNNNNNNNGG 3 cut(s) 62, 143, 464
AgsI TTSAA 4 cut(s) 52, 271, 450, 455
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 61, 136
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 60, 421
Alw44I GTGCAC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 2 cut(s) 164, 396
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AsuC2I CCSGG 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 140
BciT130I CCWGG 2 cut(s) 63, 138
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 60, 421
BfmI CTRYAG 1 cut(s) 459
BfuAI ACCTGC 2 cut(s) 452, 466
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 3 cut(s) 63, 138, 315
BmgBI CACGTC 1 cut(s) 46
BmrFI CCNGG 3 cut(s) 63, 138, 315
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpuMI CCSGG 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 143, 464
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 2 cut(s) 63, 138
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 2 cut(s) 324, 476
BseLI CCNNNNNNNGG 3 cut(s) 62, 143, 464
BseMII CTCAG 3 cut(s) 192, 416, 434
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 162, 225
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 239
BshFI GGCC 2 cut(s) 166, 398
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 314
BslI CCNNNNNNNGG 3 cut(s) 62, 143, 464
BsmAI GTCTC 2 cut(s) 60, 421
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 2 cut(s) 166, 398
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 1 cut(s) 381
BspACI CCGC 2 cut(s) 18, 468
BspANI GGCC 2 cut(s) 166, 398
BspCNI CTCAG 3 cut(s) 193, 417, 435
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 2 cut(s) 452, 466
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 63, 138
BstC8I GCNNGC 2 cut(s) 231, 419
BstDEI CTNAG 3 cut(s) 201, 425, 443
BstENI CCTNNNNNAGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 324, 476
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 2 cut(s) 60, 421
BstMBI GATC 1 cut(s) 381
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 2 cut(s) 63, 138
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 3 cut(s) 61, 136, 313
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 40
BsuRI GGCC 2 cut(s) 166, 398
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 2 cut(s) 324, 476
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 2 cut(s) 452, 466
Cac8I GCNNGC 2 cut(s) 231, 419
CaiI CAGNNNCTG 1 cut(s) 137
CsiI ACCWGGT 1 cut(s) 136
Csp6I GTAC 2 cut(s) 134, 296
CviAII CATG 1 cut(s) 418
CviJI RGCY 6 cut(s) 119, 166, 229, 233, 369, 398
CviKI_1 RGCY 6 cut(s) 119, 166, 229, 233, 369, 398
CviQI GTAC 2 cut(s) 134, 296
DdeI CTNAG 3 cut(s) 201, 425, 443
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 61, 136
FaeI CATG 1 cut(s) 421
FaiI YATR 6 cut(s) 241, 285, 347, 386, 388, 419
FatI CATG 1 cut(s) 417
FauI CCCGC 1 cut(s) 11
FauNDI CATATG 1 cut(s) 386
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 1 cut(s) 331
Fsp4HI GCNGC 1 cut(s) 129
GluI GCNGC 1 cut(s) 129
HaeIII GGCC 2 cut(s) 166, 398
HapII CCGG 1 cut(s) 314
Hin1II CATG 1 cut(s) 421
HinfI GANTC 2 cut(s) 24, 197
HpaII CCGG 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 2 cut(s) 202, 444
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 8 cut(s) 96, 256, 292, 349, 392, 417, 421, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 3 cut(s) 201, 425, 443
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 1 cut(s) 381
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 391
MabI ACCWGGT 1 cut(s) 136
MaeII ACGT 1 cut(s) 45
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 2 cut(s) 40, 419
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 1 cut(s) 450
MnlI CCTC 6 cut(s) 39, 87, 140, 143, 207, 246
MseI TTAA 2 cut(s) 339, 475
MslI CAYNNNNRTG 2 cut(s) 113, 363
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 3 cut(s) 63, 138, 315
MunI CAATTG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 2 cut(s) 63, 138
MwoI GCNNNNNNNGC 1 cut(s) 125
NciI CCSGG 1 cut(s) 315
NdeI CATATG 1 cut(s) 386
NdeII GATC 1 cut(s) 381
NlaIII CATG 1 cut(s) 421
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 452
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 24, 197
PkrI GCNGC 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 61, 136
PspGI CCWGG 2 cut(s) 61, 136
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 135, 297
RsaNI GTAC 2 cut(s) 134, 296
RseI CAYNNNNRTG 2 cut(s) 113, 363
SaqAI TTAA 2 cut(s) 339, 475
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 1 cut(s) 381
SbfI CCTGCAGG 1 cut(s) 463
ScaI AGTACT 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 63, 138, 315
SdaI CCTGCAGG 1 cut(s) 463
SduI GDGCHC 1 cut(s) 394
SetI ASST 6 cut(s) 48, 139, 235, 309, 460, 466
SexAI ACCWGGT 1 cut(s) 136
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 459
SmiMI CAYNNNNRTG 2 cut(s) 113, 363
SphI GCATGC 1 cut(s) 421
Sse8387I CCTGCAGG 1 cut(s) 463
Sse9I AATT 1 cut(s) 450
SsiI CCGC 2 cut(s) 18, 468
SspI AATATT 2 cut(s) 103, 343
StyD4I CCNGG 3 cut(s) 61, 136, 313
TaiI ACGT 1 cut(s) 48
TasI AATT 1 cut(s) 450
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 2 cut(s) 24, 197
Tru1I TTAA 2 cut(s) 339, 475
Tru9I TTAA 2 cut(s) 339, 475
TscAI CASTG 1 cut(s) 419
TseI GCWGC 1 cut(s) 128
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 1 cut(s) 419
VneI GTGCAC 1 cut(s) 390
XagI CCTNNNNNAGG 1 cut(s) 141
XceI RCATGY 1 cut(s) 421
ZrmI AGTACT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.