Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007813

Physical Location & Seq

Genomic Coordinates Reverse (-)

3269353 .. 3272388

Gene Structure 3036 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1047600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGCAATTGCAACCCCACCTCCGTCCCAAATCCATTTCCCTCCTCACCAATTCTCCACACCCACTATCTCTCTTCTCTCTCTTCGCCTTCTCTTCTCTTTCCACTCCCCTCTCTCACTCCTCTCCCATCTCCCCGTCTTCTTCTTCCTCCTCTCAATGGTTCTCCGTCCTCCGCGCTGCCATTGCCGCCGCAGACTTGCCGCTCGGGAAGCGCGTTCACGCGCTGATTATAACCTCCGGCGACGACCCGGGTCATTTCTTGACCAACAACCTCATCACCATGTATTCCAAATGCCGGTGTCTGTCAACCGCACGCCGCGTGTTCGATAAAATGCCCGGCCGAGACCTTGTCTCATGGAACTCCATTCTAGCGGCCTATGCGCAGGCTGCGGGCTCTGATGCTGACAATGTTCAAGTGGGTCTTGGCCTTTTCCGGCGTCTTCGTGAATCTGTTGTTTTTACCAGTCGGCTCACTTTGGCGCCGGTTTTGAAGCTGTGTTTGCTCTCAGGGCATGTTTGGGCTTCGGAGGCGGTTCATGGGTATGCTGTTAAGATTGGATTGGAGTGGGATGAGTTTGTTTCTGGTGCTCTTGTGAATATATATTCTAAACTCGGACGAATTAAGGCTGCCCGTGTGTTGTTTGATGGGATGATGGAAAGGGATGTGGTGTTGTGGAATACAATGCTTAAGGCATATGTGGAAATGGGTCTTGAGGAAGGACTTTCTTTCTTCTCTGCTTTTCATCGAAGTGGATTCCGTCCTGATGATGTTAGTGTGCGCTCTGTTCTCAGTGGGATTGACAGAATTGATTATTTTGAAGGCAAGAGGCACATGGAGCAGGTTCAAGCATATGCAATGAAATTGTTTTTGTATGATACTAAGTCGGAATCTTTGGACATATATTCGTGGAACAAGACATTGTCCGAGTATGTTAAAGCTGGTGAAAATTGGGCTGCTGTAGAATGTTTTATAGATATTGTAAGATCAAAAGTAGAGCTTGACAGTGTAACATTAGTTGTTATTCTGTCTGCGGTTGCAGGTGTAAATGACTTGGACTTGGGGAAACAGATTCATGGCATTGCTTTGAAATCACATTTTGATTCTGTTGTTTCTGTTGCAAATAGCCTTATCAACATGTATTCAAAGGCTGGTTCTGTTTATTCTTCAAGAAAAGTGTTCAATCGCATGAAAGAAGTGGACCTAATTTCCTGGAACTCGATGATATCATGTTGTGCACAAAGTGGTTTAGGAGAGGAAGCGGTAAACTTGTTTATGGGCTTATTACATGATGGTTTGAGACCTGATCAATTTACTGTTGCAAGTGTTTTAAGGGCTTGCTCCTCGCTTGAAGAAGGGTTGTCAGCCAGCAAACAGATTCATGTTCATGCTACAAAATCCGGCATTGTTGCTGACAGTTTTGTTTCGACGGCTCTTATTGATGTTTATTCTAGAAGTGGAAACATGGAGGATGCAGAGGTCCTCCTTGGAAACAAACTTAAATTCAATCTGGCGTCTTGGAATGCAATGATGTTCGGATACATAATGAGTAATGACTGTCACAAGGCATTGGACCTTATGAGGATGATGCACGAAGGTGGACACAGGCCGGATGAGATATCTTTAACAACTGCGGGTAAGGCTGCTAGTAGCCTGGTAGCTTTGGGACCGGGAAAGCAAATTCATGCTCATGCCATAAAAACAGGGTTTGTTTTGGATTTATGTGTTAACAGCGGAATTCTAGATATGTATATCAAATGTGGAGACATGGGAAGTGCACACACAGTTTTCAATTACATCCCTGCACCCGATGGTGTTGCATGGACAACTATGATCTCAGGATGTGTAGAAAATGGAGATGAAGTGCTCTCTCTTTCTATATACCATCAGATGAGGCAGTCACGAGTGGAGCCTGATGAATATACCTTTGCCACCCTTGTCAAAGCCAGCTCATGTTTGACAGCATTAGAACAGGGAAAACAGATACACGCTGATGCTATAAAGTTGGATTTTTCCTCGGACCCTTTTGTCGCGACATCACTTGTTGACATGTATGCCAAGTGTGGCAACATAGAAGATGCTTATCGTTTATTCAGGAGGATGGATGTTAGGAACGTCGCCCTGTGGAATGCCATGTTGGTCGGTTTAGCTCAGCACGGAAATGCTGAAGAAGCACTAAGCCTTTTCAGAGTCATGAAAATGAAGAACATCGAGCCTGATAGAGTTACTTTCATTGGAGTCCTTTCTGCTTGCAGCCATTCTGGTTTAGTTTCCGAAGCTTATGAGTATTTTTCTACAATGCAGAAAGATTACGGTGTTGAACCTGAGATTGAACACTACTCTTGCCTAGTTGATGCCCTTGGCCGTGCAGGTCGAGTCCAAGAGGCAGAAAAGCTGATAGCATCAATGCCCTTTGAAGCTTCTGCCTCAATGTATAGAGCCCTTCTAGGTGCTTGTAGAGTTAAAGGGGACACCGAAACTGGAAAACGAGTTGCAGCACAGCTCCTGGCCATGGAGCCATCTGACTCCTCCGCCTACGTGCTTTTATCCAACATCTATGCGGCTGCCAACCAATGGAATGTCGTAAATGATGCCAGAGCAATGATGCAGAAACAGAATGTAAAGAAAGAACCCGGTTTCAGCTGGATAGACGTGAAGAACAAGGTACATTTGTTTGTGGTAGACGATAAATCCCATCCACAAGCAGATGTCATACACGACAAGGTGGAGGAGATGATAAAGCGAATCGGGGAAGAAGGGTACGTCCCGGATACGGAGTTTGCACTGGTGGATGTGGAAGAAGAGGAGAAAGAGCGGTCTCTGTACTACCACAGTGAGAAGCTAGCAATAGCGTATGGACTCATAAGCACTCCTCCGTCGGCCGCCATTCGGGTGATAAAAAACCTGAGAGTTTGTGGAGATTGCCATAATGCAGTGAAATACATATCAAAGGTTTATCAGAGAGAGATTGTTTTGAGAGATGCAAATCGCTTCCATCGGTTCAAGGATGGAACTTGTTCTTGCGGTGATTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

1012

Amino Acids

111.74

Weight (kDa)

6.19

Isoelectric Point (pI)

41.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	400 - 447	8.8e-07	PPR repeat family
PPR	PF01535	402 - 431	8.5e-06	PPR repeat
PPR_2	PF13041	602 - 648	1.2e-08	PPR repeat family
PPR_long	PF17177	610 - 762	1.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	704 - 751	1.8e-10	PPR repeat family
PPR	PF01535	708 - 736	4.9e-06	PPR repeat
E_motif	PF20431	823 - 883	8.1e-18	E motif
DYW_deaminase	PF14432	919 - 1011	5.5e-44	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33170
fragaria_vesca	FvH4_2g30790
malus_domestica	MD08G1046200.v1.1 MD15G1047600.v1.1
prunus_persica	Prupe.1G402000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0312061 RchiOBHm_Chr6g0312101 RchiOBHm_Chr7g0217531
rosa_laevigata	RLG00000010354 RLG00000010356
rosa_multiflora	Rmu_sc0001501.1_g000037
rosa_roxburghii	Rroxscaffold_7G00157070 Rroxscaffold_7G00157080
rosa_rugosa	Rorug06G0392600
rosa_samantha	Rh6AG509100 Rh6AG509400 Rh6BG520400 Rh6BG520500 Rh6CG526300 Rh6CG526400 Rh6DG511800 Rh6DG511900
rosa_wichuraiana	Rw6G044330 Rw6G044340

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	230
AarI	CACCTGC	1 cut(s)	1028
AasI	GACNNNNNNGTC	1 cut(s)	2024
Acc16I	TGCGCA	1 cut(s)	381
Acc36I	ACCTGC	3 cut(s)	829, 1028, 2357
AccB1I	GGYRCC	1 cut(s)	478
AccB7I	CCANNNNNTGG	1 cut(s)	2571
AccBSI	CCGCTC	2 cut(s)	202, 2812
AccI	GTMKAC	1 cut(s)	2679
AccII	CGCG	5 cut(s)	174, 213, 221, 318, 2030
AcoI	YGGCCR	4 cut(s)	337, 2359, 2505, 2877
AcsI	RAATTY	3 cut(s)	1499, 1677, 1734
AcuI	CTGAAG	1 cut(s)	2184
AcyI	GRCGYC	3 cut(s)	436, 479, 1511
AdeI	CACNNNGTG	1 cut(s)	1241
AfaI	GTAC	3 cut(s)	2664, 2759, 2822
AfiI	CCNNNNNNNGG	6 cut(s)	156, 294, 2509, 2571, 2770, 2886
AflII	CTTAAG	1 cut(s)	686
AflIII	ACRYGT	2 cut(s)	1134, 2046
AjiI	CACGTC	1 cut(s)	2650
AjnI	CCWGG	3 cut(s)	1208, 1650, 2502
AjuI	GAANNNNNNNTTGG	2 cut(s)	2117, 2149
AleI	CACNNNNGTG	1 cut(s)	1593
Alw21I	GWGCWC	4 cut(s)	589, 1237, 1777, 1866
Alw26I	GTCTC	5 cut(s)	336, 355, 1291, 1755, 2820
Alw44I	GTGCAC	2 cut(s)	1233, 1773
AlwNI	CAGNNNCTG	1 cut(s)	2503
Ama87I	CYCGRG	2 cut(s)	203, 247
AoxI	GGCC	7 cut(s)	337, 372, 424, 1604, 2359, 2505, 2877
ApaLI	GTGCAC	2 cut(s)	1233, 1773
ApeKI	GCWGC	8 cut(s)	176, 386, 626, 953, 1640, 2250, 2492, 2561
ApoI	RAATTY	3 cut(s)	1499, 1677, 1734
Asp700I	GAANNNNTTC	1 cut(s)	3013
AspLEI	GCGC	6 cut(s)	176, 213, 223, 382, 481, 780
AspS9I	GGNCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
AsuC2I	CCSGG	6 cut(s)	248, 249, 336, 1668, 2631, 2765
AsuHPI	GGTGA	5 cut(s)	37, 268, 953, 2902, 3035
AsuNHI	GCTAGC	1 cut(s)	2839
AvaI	CYCGRG	2 cut(s)	203, 247
AvaII	GGWCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
BaeGI	GKGCMC	2 cut(s)	1237, 1777
BaeI	ACNNNNGTAYC	2 cut(s)	2654, 2687
BalI	TGGCCA	1 cut(s)	2507
BanI	GGYRCC	1 cut(s)	478
BanII	GRGCYC	2 cut(s)	395, 2440
BauI	CACGAG	1 cut(s)	1899
BbsI	GAAGAC	2 cut(s)	129, 431
Bbv12I	GWGCWC	4 cut(s)	589, 1237, 1777, 1866
BbvI	GCAGC	8 cut(s)	163, 373, 613, 940, 1627, 2262, 2504, 2548
BceAI	ACGGC	2 cut(s)	1443, 2346
BcgI	CGANNNNNNTGC	2 cut(s)	1796, 1830
BciT130I	CCWGG	3 cut(s)	1210, 1652, 2504
BciVI	GTATCC	2 cut(s)	1529, 2761
BclI	TGATCA	1 cut(s)	1303
BcnI	CCSGG	6 cut(s)	248, 249, 336, 1668, 2631, 2765
BcoDI	GTCTC	5 cut(s)	336, 355, 1291, 1755, 2820
BfaI	CTAG	7 cut(s)	368, 1449, 1644, 1739, 2345, 2444, 2840
BfmI	CTRYAG	1 cut(s)	957
BfoI	RGCGCY	1 cut(s)	482
BfrI	CTTAAG	1 cut(s)	686
BfuAI	ACCTGC	3 cut(s)	829, 1028, 2357
BfuI	GTATCC	2 cut(s)	1529, 2761
BlpI	GCTNAGC	1 cut(s)	2148
Bme1390I	CCNGG	9 cut(s)	248, 249, 336, 1210, 1652, 1668, 2504, 2631, 2765
Bme18I	GGWCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
BmeT110I	CYCGRG	2 cut(s)	203, 247
BmgBI	CACGTC	1 cut(s)	2650
BmgT120I	GGNCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
BmiI	GGNNCC	5 cut(s)	480, 1665, 1908, 2019, 2514
BmrFI	CCNGG	9 cut(s)	248, 249, 336, 1210, 1652, 1668, 2504, 2631, 2765
BmtI	GCTAGC	1 cut(s)	2843
BoxI	GACNNNNGTC	1 cut(s)	249
BpiI	GAAGAC	2 cut(s)	129, 431
Bpu1102I	GCTNAGC	1 cut(s)	2148
BpuEI	CTTGAG	1 cut(s)	731
BpuMI	CCSGG	6 cut(s)	248, 249, 336, 1668, 2631, 2765
BsaAI	YACGTR	1 cut(s)	2536
BsaBI	GATNNNNATC	2 cut(s)	2079, 2982
BsaHI	GRCGYC	3 cut(s)	436, 479, 1511
BsaI	GGTCTC	3 cut(s)	336, 1291, 2820
BsaJI	CCNNGG	5 cut(s)	247, 1483, 2013, 2356, 2508
BsaXI	ACNNNNNCTCC	5 cut(s)	33, 37, 67, 1845, 1875
Bsc4I	CCNNNNNNNGG	6 cut(s)	156, 294, 2509, 2571, 2770, 2886
Bse118I	RCCGGY	2 cut(s)	294, 481
Bse1I	ACTGG	4 cut(s)	462, 2482, 2787, 3035
Bse3DI	GCAATG	5 cut(s)	180, 861, 1077, 1530, 2604
Bse8I	GATNNNNATC	2 cut(s)	2079, 2982
BseBI	CCWGG	3 cut(s)	1210, 1652, 2504
BseDI	CCNNGG	5 cut(s)	247, 1483, 2013, 2356, 2508
BseJI	GATNNNNATC	2 cut(s)	2079, 2982
BseLI	CCNNNNNNNGG	6 cut(s)	156, 294, 2509, 2571, 2770, 2886
BseMI	GCAATG	5 cut(s)	180, 861, 1077, 1530, 2604
BseMII	CTCAG	6 cut(s)	519, 802, 1848, 2162, 2313, 2894
BseNI	ACTGG	4 cut(s)	462, 2482, 2787, 3035
BseRI	GAGGAG	8 cut(s)	32, 109, 139, 1330, 2515, 2741, 2816, 2859
BseSI	GKGCMC	2 cut(s)	1237, 1777
BseX3I	CGGCCG	2 cut(s)	337, 2877
BseXI	GCAGC	8 cut(s)	163, 373, 613, 940, 1627, 2262, 2504, 2548
BsgI	GTGCAG	2 cut(s)	1785, 2385
Bsh1236I	CGCG	5 cut(s)	174, 213, 221, 318, 2030
Bsh1285I	CGRYCG	2 cut(s)	340, 2880
BshFI	GGCC	7 cut(s)	339, 374, 426, 1606, 2361, 2507, 2879
BshNI	GGYRCC	1 cut(s)	478
BsiEI	CGRYCG	2 cut(s)	340, 2880
BsiHKAI	GWGCWC	4 cut(s)	589, 1237, 1777, 1866
BsiHKCI	CYCGRG	2 cut(s)	203, 247
BslFI	GGGAC	4 cut(s)	9, 1677, 2480, 2747
BslI	CCNNNNNNNGG	6 cut(s)	156, 294, 2509, 2571, 2770, 2886
BsmAI	GTCTC	5 cut(s)	336, 355, 1291, 1755, 2820
BsmFI	GGGAC	4 cut(s)	9, 1677, 2480, 2747
BsmI	GAATGC	2 cut(s)	1525, 2131
BsnI	GGCC	7 cut(s)	339, 374, 426, 1606, 2361, 2507, 2879
Bso31I	GGTCTC	3 cut(s)	336, 1291, 2820
BsoBI	CYCGRG	2 cut(s)	203, 247
Bsp1286I	GDGCHC	6 cut(s)	395, 589, 1237, 1777, 1866, 2440
Bsp143I	GATC	3 cut(s)	983, 1303, 1830
Bsp1720I	GCTNAGC	1 cut(s)	2148
Bsp19I	CCATGG	1 cut(s)	2508
Bsp68I	TCGCGA	1 cut(s)	2030
BspANI	GGCC	7 cut(s)	339, 374, 426, 1606, 2361, 2507, 2879
BspCNI	CTCAG	6 cut(s)	518, 801, 1847, 2161, 2314, 2895
BspFNI	CGCG	5 cut(s)	174, 213, 221, 318, 2030
BspHI	TCATGA	1 cut(s)	2190
BspLI	GGNNCC	5 cut(s)	480, 1665, 1908, 2019, 2514
BspMI	ACCTGC	3 cut(s)	829, 1028, 2357
BspOI	GCTAGC	1 cut(s)	2843
BspT107I	GGYRCC	1 cut(s)	478
BspTI	CTTAAG	1 cut(s)	686
BspTNI	GGTCTC	3 cut(s)	336, 1291, 2820
BsrBI	CCGCTC	2 cut(s)	202, 2812
BsrDI	GCAATG	5 cut(s)	180, 861, 1077, 1530, 2604
BsrFI	RCCGGY	2 cut(s)	294, 481
BsrI	ACTGG	4 cut(s)	462, 2482, 2787, 3035
BssAI	RCCGGY	2 cut(s)	294, 481
BssECI	CCNNGG	5 cut(s)	247, 1483, 2013, 2356, 2508
BssMI	GATC	3 cut(s)	983, 1303, 1830
BssNI	GRCGYC	3 cut(s)	436, 479, 1511
BssSI	CACGAG	1 cut(s)	1899
BssT1I	CCWWGG	3 cut(s)	1483, 2356, 2508
Bst2BI	CACGAG	1 cut(s)	1899
Bst2UI	CCWGG	3 cut(s)	1210, 1652, 2504
Bst4CI	ACNGT	7 cut(s)	1004, 1315, 1415, 1556, 1783, 2312, 2831
Bst6I	CTCTTC	4 cut(s)	77, 86, 97, 2793
BstACI	GRCGYC	3 cut(s)	436, 479, 1511
BstAFI	CTTAAG	1 cut(s)	686
BstBAI	YACGTR	1 cut(s)	2536
BstC8I	GCNNGC	8 cut(s)	313, 384, 391, 1336, 1366, 1945, 2248, 2841
BstDEI	CTNAG	8 cut(s)	505, 788, 879, 1834, 2148, 2174, 2322, 2903
BstDSI	CCRYGG	1 cut(s)	2508
BstFNI	CGCG	5 cut(s)	174, 213, 221, 318, 2030
BstH2I	RGCGCY	1 cut(s)	482
BstHHI	GCGC	6 cut(s)	176, 213, 223, 382, 481, 780
BstKTI	GATC	3 cut(s)	986, 1306, 1833
BstMAI	GTCTC	5 cut(s)	336, 355, 1291, 1755, 2820
BstMBI	GATC	3 cut(s)	983, 1303, 1830
BstMCI	CGRYCG	2 cut(s)	340, 2880
BstNI	CCWGG	3 cut(s)	1210, 1652, 2504
BstNSI	RCATGY	3 cut(s)	515, 1138, 2050
BstPAI	GACNNNNGTC	1 cut(s)	249
BstSCI	CCNGG	9 cut(s)	246, 247, 334, 1208, 1650, 1666, 2502, 2629, 2763
BstSFI	CTRYAG	1 cut(s)	957
BstSLI	GKGCMC	2 cut(s)	1237, 1777
BstUI	CGCG	5 cut(s)	174, 213, 221, 318, 2030
BstV1I	GCAGC	8 cut(s)	163, 373, 613, 940, 1627, 2262, 2504, 2548
BstV2I	GAAGAC	2 cut(s)	129, 431
BstZI	CGGCCG	2 cut(s)	337, 2877
BsuI	GTATCC	2 cut(s)	1529, 2761
BsuRI	GGCC	7 cut(s)	339, 374, 426, 1606, 2361, 2507, 2879
BtgI	CCRYGG	1 cut(s)	2508
BtrI	CACGTC	1 cut(s)	2650
BtsI	GCAGTG	1 cut(s)	2937
BtsIMutI	CAGTG	5 cut(s)	796, 1009, 2780, 2836, 2937
BtuMI	TCGCGA	1 cut(s)	2030
BveI	ACCTGC	3 cut(s)	829, 1028, 2357
Cac8I	GCNNGC	8 cut(s)	313, 384, 391, 1336, 1366, 1945, 2248, 2841
CaiI	CAGNNNCTG	1 cut(s)	2503
CciI	TCATGA	1 cut(s)	2190
CfoI	GCGC	6 cut(s)	176, 213, 223, 382, 481, 780
Cfr10I	RCCGGY	2 cut(s)	294, 481
Cfr13I	GGNCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
Cfr9I	CCCGGG	1 cut(s)	247
CseI	GACGC	2 cut(s)	425, 1500
Csp6I	GTAC	3 cut(s)	2663, 2758, 2821
CviQI	GTAC	3 cut(s)	2663, 2758, 2821
DdeI	CTNAG	8 cut(s)	505, 788, 879, 1834, 2148, 2174, 2322, 2903
DinI	GGCGCC	1 cut(s)	480
DpnI	GATC	3 cut(s)	985, 1305, 1832
DpnII	GATC	3 cut(s)	983, 1303, 1830
DraIII	CACNNNGTG	1 cut(s)	1241
DrdI	GACNNNNNNGTC	1 cut(s)	2024
DseDI	GACNNNNNNGTC	1 cut(s)	2024
EaeI	YGGCCR	4 cut(s)	337, 2359, 2505, 2877
EagI	CGGCCG	2 cut(s)	337, 2877
Eam1104I	CTCTTC	4 cut(s)	77, 86, 97, 2793
EarI	CTCTTC	4 cut(s)	77, 86, 97, 2793
EciI	GGCGGA	1 cut(s)	2518
EclXI	CGGCCG	2 cut(s)	337, 2877
Eco130I	CCWWGG	3 cut(s)	1483, 2356, 2508
Eco24I	GRGCYC	2 cut(s)	395, 2440
Eco31I	GGTCTC	3 cut(s)	336, 1291, 2820
Eco32I	GATATC	2 cut(s)	1224, 1617
Eco47I	GGWCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
Eco52I	CGGCCG	2 cut(s)	337, 2877
Eco57I	CTGAAG	1 cut(s)	2184
Eco88I	CYCGRG	2 cut(s)	203, 247
EcoO109I	RGGNCCY	1 cut(s)	1477
EcoRI	GAATTC	1 cut(s)	1734
EcoRII	CCWGG	3 cut(s)	1208, 1650, 2502
EcoRV	GATATC	2 cut(s)	1224, 1617
EcoT14I	CCWWGG	3 cut(s)	1483, 2356, 2508
EcoT38I	GRGCYC	2 cut(s)	395, 2440
EgeI	GGCGCC	1 cut(s)	480
EheI	GGCGCC	1 cut(s)	480
ErhI	CCWWGG	3 cut(s)	1483, 2356, 2508
FalI	AAGNNNNNCTT	4 cut(s)	405, 437, 981, 1013
FaqI	GGGAC	4 cut(s)	9, 1677, 2480, 2747
FauI	CCCGC	2 cut(s)	382, 1624
FauNDI	CATATG	2 cut(s)	694, 850
FbaI	TGATCA	1 cut(s)	1303
FblI	GTMKAC	1 cut(s)	2679
FriOI	GRGCYC	2 cut(s)	395, 2440
FspBI	CTAG	7 cut(s)	368, 1449, 1644, 1739, 2345, 2444, 2840
FspI	TGCGCA	1 cut(s)	381
GlaI	GCGC	6 cut(s)	175, 212, 222, 381, 480, 779
HaeII	RGCGCY	1 cut(s)	482
HaeIII	GGCC	7 cut(s)	339, 374, 426, 1606, 2361, 2507, 2879
HgaI	GACGC	2 cut(s)	425, 1500
HhaI	GCGC	6 cut(s)	176, 213, 223, 382, 481, 780
Hin1I	GRCGYC	3 cut(s)	436, 479, 1511
Hin6I	GCGC	6 cut(s)	174, 211, 221, 380, 479, 778
HinP1I	GCGC	6 cut(s)	174, 211, 221, 380, 479, 778
HincII	GTYRAC	3 cut(s)	306, 1726, 2044
HindII	GTYRAC	3 cut(s)	306, 1726, 2044
HindIII	AAGCTT	2 cut(s)	2274, 2415
HpaI	GTTAAC	1 cut(s)	1726
HphI	GGTGA	5 cut(s)	37, 268, 953, 2902, 3035
Hpy99I	CGWCG	4 cut(s)	245, 1429, 2117, 2878
HpyAV	CCTTC	7 cut(s)	97, 710, 812, 1346, 1586, 2450, 2747
HpyCH4III	ACNGT	7 cut(s)	1004, 1315, 1415, 1556, 1783, 2312, 2831
HpyCH4IV	ACGT	4 cut(s)	2112, 2535, 2649, 2760
HpyF3I	CTNAG	8 cut(s)	505, 788, 879, 1834, 2148, 2174, 2322, 2903
HpySE526I	ACGT	4 cut(s)	2112, 2535, 2649, 2760
Hsp92I	GRCGYC	3 cut(s)	436, 479, 1511
HspAI	GCGC	6 cut(s)	174, 211, 221, 380, 479, 778
KasI	GGCGCC	1 cut(s)	478
Ksp22I	TGATCA	1 cut(s)	1303
KspAI	GTTAAC	1 cut(s)	1726
Kzo9I	GATC	3 cut(s)	983, 1303, 1830
LmnI	GCTCC	5 cut(s)	835, 1343, 1906, 2505, 2512
Lsp1109I	GCAGC	8 cut(s)	163, 373, 613, 940, 1627, 2262, 2504, 2548
MaeI	CTAG	7 cut(s)	368, 1449, 1644, 1739, 2345, 2444, 2840
MaeII	ACGT	4 cut(s)	2112, 2535, 2649, 2760
MaeIII	GTNAC	4 cut(s)	1006, 1556, 1896, 2221
MalI	GATC	3 cut(s)	985, 1305, 1832
MbiI	CCGCTC	2 cut(s)	202, 2812
MboI	GATC	3 cut(s)	983, 1303, 1830
MfeI	CAATTG	1 cut(s)	5
MhlI	GDGCHC	6 cut(s)	395, 589, 1237, 1777, 1866, 2440
MlsI	TGGCCA	1 cut(s)	2507
MluNI	TGGCCA	1 cut(s)	2507
Mly113I	GGCGCC	1 cut(s)	479
MlyI	GAGTC	5 cut(s)	2196, 2244, 2382, 2516, 2850
MmeI	TCCRAC	3 cut(s)	864, 1983, 2571
Mox20I	TGGCCA	1 cut(s)	2507
MroXI	GAANNNNTTC	1 cut(s)	3013
MscI	TGGCCA	1 cut(s)	2507
MseI	TTAA	9 cut(s)	549, 621, 687, 933, 1328, 1497, 1622, 1725, 2460
Msp20I	TGGCCA	1 cut(s)	2507
MspA1I	CMGCKG	2 cut(s)	1731, 2640
MspCI	CTTAAG	1 cut(s)	686
MspR9I	CCNGG	9 cut(s)	248, 249, 336, 1210, 1652, 1668, 2504, 2631, 2765
MunI	CAATTG	1 cut(s)	5
Mva1269I	GAATGC	2 cut(s)	1525, 2131
MvaI	CCWGG	3 cut(s)	1210, 1652, 2504
MvnI	CGCG	5 cut(s)	174, 213, 221, 318, 2030
NarI	GGCGCC	1 cut(s)	479
NciI	CCSGG	6 cut(s)	248, 249, 336, 1668, 2631, 2765
NcoI	CCATGG	1 cut(s)	2508
NdeI	CATATG	2 cut(s)	694, 850
NdeII	GATC	3 cut(s)	983, 1303, 1830
NheI	GCTAGC	1 cut(s)	2839
NlaIV	GGNNCC	5 cut(s)	480, 1665, 1908, 2019, 2514
NmeAIII	GCCGAG	1 cut(s)	365
NmuCI	GTSAC	2 cut(s)	1556, 1896
NruI	TCGCGA	1 cut(s)	2030
NsbI	TGCGCA	1 cut(s)	381
NspI	RCATGY	3 cut(s)	515, 1138, 2050
OliI	CACNNNNGTG	1 cut(s)	1593
PagI	TCATGA	1 cut(s)	2190
PaqCI	CACCTGC	1 cut(s)	1028
PciI	ACATGT	2 cut(s)	1134, 2046
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PctI	GAATGC	2 cut(s)	1525, 2131
PdmI	GAANNNNTTC	1 cut(s)	3013
PfeI	GAWTC	7 cut(s)	446, 753, 887, 1069, 1100, 1375, 2742
PflFI	GACNNNGTC	2 cut(s)	347, 919
PflMI	CCANNNNNTGG	1 cut(s)	2571
PfoI	TCCNGGA	2 cut(s)	1208, 2763
PleI	GAGTC	5 cut(s)	2195, 2243, 2381, 2516, 2850
PluTI	GGCGCC	1 cut(s)	482
PpsI	GAGTC	5 cut(s)	2195, 2243, 2381, 2516, 2850
Ppu21I	YACGTR	1 cut(s)	2536
PpuMI	RGGWCCY	1 cut(s)	1477
PscI	ACATGT	2 cut(s)	1134, 2046
PshAI	GACNNNNGTC	1 cut(s)	249
PsiI	TTATAA	1 cut(s)	230
Psp5II	RGGWCCY	1 cut(s)	1477
Psp6I	CCWGG	3 cut(s)	1208, 1650, 2502
PspGI	CCWGG	3 cut(s)	1208, 1650, 2502
PspN4I	GGNNCC	5 cut(s)	480, 1665, 1908, 2019, 2514
PspPI	GGNCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
PspPPI	RGGWCCY	1 cut(s)	1477
PstNI	CAGNNNCTG	1 cut(s)	2503
PsyI	GACNNNGTC	2 cut(s)	347, 919
PvuII	CAGCTG	1 cut(s)	2640
RruI	TCGCGA	1 cut(s)	2030
RsaI	GTAC	3 cut(s)	2664, 2759, 2822
RsaNI	GTAC	3 cut(s)	2663, 2758, 2821
SaqAI	TTAA	9 cut(s)	549, 621, 687, 933, 1328, 1497, 1622, 1725, 2460
Sau3AI	GATC	3 cut(s)	983, 1303, 1830
Sau96I	GGNCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
SchI	GAGTC	5 cut(s)	2196, 2244, 2382, 2516, 2850
ScrFI	CCNGG	9 cut(s)	248, 249, 336, 1210, 1652, 1668, 2504, 2631, 2765
SduI	GDGCHC	6 cut(s)	395, 589, 1237, 1777, 1866, 2440
SfcI	CTRYAG	1 cut(s)	957
SfoI	GGCGCC	1 cut(s)	480
SinI	GGWCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
SmaI	CCCGGG	1 cut(s)	249
SmlI	CTYRAG	2 cut(s)	686, 710
SmoI	CTYRAG	2 cut(s)	686, 710
SspDI	GGCGCC	1 cut(s)	478
SspMI	CTAG	7 cut(s)	368, 1449, 1644, 1739, 2345, 2444, 2840
StyD4I	CCNGG	9 cut(s)	246, 247, 334, 1208, 1650, 1666, 2502, 2629, 2763
StyI	CCWWGG	3 cut(s)	1483, 2356, 2508
TaaI	ACNGT	7 cut(s)	1004, 1315, 1415, 1556, 1783, 2312, 2831
TaiI	ACGT	4 cut(s)	2115, 2538, 2652, 2763
TaqI	TCGA	6 cut(s)	324, 745, 1217, 1424, 2208, 2371
TatI	WGTACW	1 cut(s)	2820
TauI	GCSGC	7 cut(s)	188, 191, 202, 318, 374, 2561, 2882
TfiI	GAWTC	7 cut(s)	446, 753, 887, 1069, 1100, 1375, 2742
Tru1I	TTAA	9 cut(s)	549, 621, 687, 933, 1328, 1497, 1622, 1725, 2460
Tru9I	TTAA	9 cut(s)	549, 621, 687, 933, 1328, 1497, 1622, 1725, 2460
TscAI	CASTG	5 cut(s)	796, 1009, 2787, 2836, 2937
TseFI	GTSAC	2 cut(s)	1556, 1896
TseI	GCWGC	8 cut(s)	176, 386, 626, 953, 1640, 2250, 2492, 2561
Tsp45I	GTSAC	2 cut(s)	1556, 1896
TspGWI	ACGGA	6 cut(s)	11, 154, 746, 2169, 2786, 2862
TspMI	CCCGGG	1 cut(s)	247
TspRI	CASTG	5 cut(s)	796, 1009, 2787, 2836, 2937
Tth111I	GACNNNGTC	2 cut(s)	347, 919
Van91I	CCANNNNNTGG	1 cut(s)	2571
Vha464I	CTTAAG	1 cut(s)	686
VneI	GTGCAC	2 cut(s)	1233, 1773
VpaK11BI	GGWCC	5 cut(s)	1198, 1477, 1570, 1664, 2017
XapI	RAATTY	3 cut(s)	1499, 1677, 1734
XbaI	TCTAGA	2 cut(s)	1448, 1738
XceI	RCATGY	3 cut(s)	515, 1138, 2050
XmaI	CCCGGG	1 cut(s)	247
XmiI	GTMKAC	1 cut(s)	2679
XmnI	GAANNNNTTC	1 cut(s)	3013
XspI	CTAG	7 cut(s)	368, 1449, 1644, 1739, 2345, 2444, 2840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.