Rw6G044330

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007813
Physical Location & Seq
Forward (+)
67443455 .. 67450664
7210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044330.1

Sequence Viewer

Length: 4710 bp
ATGAGCTTGTCCAAGAAAGGATCCTTTACGAATGACACAAAGCTAGGCGCTGCTGCAAGCAAGGCAACTACTTTAAATCCAAATGCAGCAGAGTTTGTTCCCTTTTCCCTCAGATCTCCTTCATCTGGAAGCACCAGCACACCTCAGGCATTTTCTGCATCTGGAACCTTTGGAAAAGCAGTATTAGATCGAACAGAGTCTTCCACCTCGAATAATTCTGATGACGAGGCCCACCAATTTTGGCGCCACCAGCTCCCTGATGATATCACCCCTGACTTTAAGGTCATGGGAGAAGATGAGTCACCAGGTCTTGGAAACATCTCTTTGGCGGGATTGTCCTTGCATGATGACAGTGAAGCAACACAGTTTCCTGCTTCTACAGGCAGTGGATACTTATATAATGACATTAATGGTAATAGCTTTTCTGAAAAGTTAAGAGTTGCTACATCCCCCTATGGGGATGATCTTTCCTCGCCCAGTTTCATGCATATGTCAACCAAGCCTTGGGATAAGCAGATTCTGAATAGCAATCAGCTTGTTAGCAATGGTCATGAGGGGTCTCTTTATGATGGCATTTCTAGACATGGATTCATGAATGACATATTGGGTGAGCAACCAGTTGTTGATGATAGCGAAATTAACCCTGCCGAATTTTTGGGTTCACAGTTCCCCGGGTTTTCCTCTCAGAGCCTTGCAGAAGTTTACTTTGCCAGTGGTCTTCAAGTTGATGGTGGTTTTAATGAAAATATGAATCCAAAGACCTTGTCAGCTCCTGATCTTAGTTCAATGGACTTTCCTGCACTAACTCCAACAGATGGTCTGCATGGTCATCCAAAATATGTTGGAGACGATGATTTCCAGCAAAGTGGCAATCCATATCGAACTTCTGACAAGGACAGAATGCTGATGTTTAAGTCTAGCTCATCCATTCCGTCTAGAGGTTCAATAGATTTTGCTTCAGCTGTCAGGAAATTGGCGACTCAGGATTCTGGTGGATGGAAGTATGAGAGGAATGGGTCTGCTGATGCCGCTATTGGCTCAAGTAGAGCTTCCCATCTTGTAAATAGCACTTACAATACTGGGCATGGAAGAGCCATCTACAGTGATAGGTCGCAGACTCGTGGTTCAGCTCGGACTGCTCCTGTTTGGCTTGAAACTGGAGATGCTGTTGCAAATATGTATACCGATCTGCGGGAGGAAGCTCGTGATCATGCACGCCTACGGAATGCATACTTTGAGCAGGCACGACAAGCATACCTTGTTGGAAACAAGGCTCTAGCAAAGGAACTGAGTGTTAAAGGGCAGCTGCATAACATGCATATGAAGGCAGCTCATGGAAAAGCTCAAGAATCTATATATCGCCAGAGGAACCCAGAAATGCAAGGCAATGGAAGACAGGAGAGGATGATAGACCTGCATGGACTTCACGTAAGTGAAGCTCTTCATAGGCTCAAGCATGAGCTGAGCATATTGAGGAACACTGCTAGAGGAGCAGAGACGGGTCTTCAGGTTTATATATGTGTTGGAACAGGCCACCACACCCGGGGTTCACGCACTCCGGCAAGGCTTCCAGTTGCTGTACAGCGTTACCTACTGGAAGAAGAGGGGCTCAACTACAATGAGCCACAGCCAGGGCTGCTTCGAGTTCTGATGGAAACCAGACAATGCACCCAAGAAATAATGCAGTTGCAAACCAATCTCCGACCCAAATCAATTTTCTTCTCCACCAAAAACCCCAAAACCCTTCTCCCTCTCTTCAATTTCTCTTCTGTCTCCAACCCCATCTCTCACTCGCAACCCTCTTCTTCATCTTCTCCATGGTTCTCTATCCTCCGCTCCGCCCGCGCCCATGCCCTTATGTTAACCTCAGGGGAATGCCAAGACCTTTTTCTAATCAACAATCTCATCACCATGTATTCGAAATGCAGGTCGCTCACGTATGCACGCCATCTGTTCGATAAAAGTCCTGACCGAGACCTGGTCACCTGGAATGCCATTCTGGCAGCCTATGCGCAAGCTGGGGGCTCTGTGGTTGAGAATGTTCAAGAGGGGCTTGGCCTTTTCCGGCGGCTTCGCGAGTCTGTTGTGTTCACCAGTCGGCACACTTTGGCTTCCGTTTTGAAGCTGTGTTTGCTGTCGGGGCGTGTTTGGATTTCGGAGGCTGTTCAGGGTTACTCTGTGAAAGTTGGGTTGGAGTGGGATGTGTTTGTTTCGGGTACTCTGGTGAATGTGTATTGTAAACTCAGAAGAGTTAAGGAGGCTCGGGCTTTGTTTGATGGGATGCTGGAAAGGGATGTTGTGTTGTGGAACACAATGCTGAAGGCATATGTGGAAATGGGTCTTCATGAAGAAGCACTCGCTCTTTTCTCCAAGTTTCATCGGAGTGGATTGGGTCCTGATGATGTCAGTGTGCGTTGTGTTCTTAGTGGGGTTCACAAAGTTGATTATGATGAAGGCAGGAGGCACATGGAGGAGGTTCAAGCATATGCAGCAAAGCTGGGGGAGTTTATAACTAATGTGGATGATGAAGCCCATCCACCAAGCTTATGTGTTCCCACTACTAATTCCGACATATTTTTGTGGAACAAGACATTGTCCAAGTATATCCGAGCTGGTGAAAATTGGGCTGCGATAGAGTTCTTTAGAAATATGATTAGATCAGAAGTGGGATATGACAATGTGACATTAGTAATTATTCTATCTGCGATTGTAGGCACAAATAATTTGGAGGTGGGGAAACAGATTCATGGTGCTGCTTTGAAATCAGATTTTGGTTCTGTTCTTTTGGTAGCAAATAGCCTTATCAGCATGTATTCAAAGGCTCGCTGTGTTCACTTTGCGAGACAAGTATTCAATCACATGAAAGAAGTGGACCTAGTTTCATGGAACTCGATGATTTCATGTTATGCTCAAAGCGGTTCAGGAGAGGAATCAATAAATCTATTTATAGGTTTATTACGGGATGGTCTAAAACCTGATCAATTTACAATTGCAAGTATTTTAAGGGCTTGCTCCTCTCTTCAAGAAGGGTTGCATCTCAGTAAACAGGTTCATGTTCATGCAATAAAGAACGGTATCGCTGCTGACAGATTTGTTTCAACAGCTCTTATTGATGTTTATTCTAGAAGTGGAAAGATGGACGAGGCAGAGGCCCTTTTAGATAACAAAGTTAAATTCGATCTGGCATCTTGGAATGCGTTGATGTTTGGATACATAAAGAGTAATGACAGTCACAAAGCACTACAACTTATGAGGATGATTCATGAAGGTGGAAAGAGAGTAGATGAGATTACTTTGGCAACTGTTGCTAAGGCCACTAGTTGCCTGGTAGCGTTGGTGCCGGGGAAGCAAATTCATGCTCATGTCATAAAAACAGGGTATTCGAAGGACTTATATGTTAACAGCAGTATTCTAGATATGTACATAAAATGTGGAGACATGAAAAGTGCACACACAGTTTTCAATGACATTCCTGCACCTGATGATGTTGCGTGGACAACTCTAATCTCAGGATGTTTAGAAAATGGAGATGAAGGGCGCTCTGTGTCTATATACCATCAGATGAGGCAGTCAGGAGTTCAGCCTGATGAATACACCTTAGCTACCCTAGTCAAAGCCGCCTCTTGTCTGACAGCATTGGAACAAGGGAAACAGATACATGCAGATGCAATAAAGTTGGAATATTCCTCAGACCCTTATGTTGCGACATCACTTGTTGACATGTACGCCAAGTGCGGTAACATAGAAGGTGCATATCGTTTATTCAGAAGGATGGATGTCGGGAGCATTGTCCTGTGGAATGCCATGTTGGTGGGTTTAGCTCAACATGGAGACGCTGAAGAAGCTCTAAATCTTTTCAGAGTCATGAAATCTAATAACATTTTGCCTGATAGAGTTACTTTCATTGGAGTTCTTTCTGCTTGCAGCCACTCTGGTTTAGTTTCTGAAGCTCACGAGCATTTTTCTTCAATGCAAAGAGATTACGGCATTGAACCTGAGATCGAGCACTACTCTTGTCTAGTTGATGCCCTTGGCCGCGCAGGGCGTGTTCAAGAGGCAGAAAAGCTGATAGCATCAATACCATTTGAAGCTTCTGCTTCAATGTACAGAGCCCTGCTTGGTGCTTGCAGGGTTCAAGGGGACACAGAAACAGGAAAACGAGTCGCAGAACAGCTCTTGGCTATTGAGCCAACTGACTCATCCGCGTATGTTCTCATATCCAATCTGAATGCTGCTGCCAACCAGTGGGATGTTGTGAACGATGCTCGTCAAATGATGAAACAGAAGAATGTAAAGAAAGAACCAGGTTTCAGTTGGATGGATGTGAAGAACAAGGTCCATTTGTTTGTAGTAGATGACAAATCCCATCCTGAAGCCGATTTGATATACGACAAGTTGGAGGACTTGATGAAACGGATCGGTGAAGAAGGGTATGTCCCTGACACAGAGTTTGTGCTTGCTGATGTTGAAGAAGAGGAAAAAGAGCGAGCCCTATATTACCATAGTGAGAGGCTAGCAATAGCTTATGGACTGATAAGCACTCCCTCATCTGCCATTATTCGGGTCATTAAGAACCTTAGAGTTTGTGGAGACTGCCATAATGCAATCAAATACATATCAAAGGTTTCTCAAAGAGAAATTGTGGTAAGAGATGCAAATCGCTTCCATCGTTTCAGAAATGGGAGCTGCTCCTGTGGTGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 PAM2 PPR PPR_2 Smr

Protein Analysis

1569

Amino Acids

173.57

Weight (kDa)

6.22

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 394 - 457 3.4e-18 Domain of unknown function (DUF1771)
PPR_2 PF13041 764 - 812 5.6e-08 PPR repeat family
PPR_2 PF13041 958 - 1005 5.6e-07 PPR repeat family
PPR_2 PF13041 1160 - 1206 9.4e-09 PPR repeat family
PPR_2 PF13041 1262 - 1309 5.4e-10 PPR repeat family
PPR PF01535 1264 - 1294 4.2e-06 PPR repeat
E_motif PF20431 1381 - 1440 1.1e-14 E motif
Eplus_motif PF20430 1446 - 1473 1.9e-09 E+ motif
DYW_deaminase PF14432 1477 - 1569 6.1e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2510
AasI GACNNNNNNGTC 1 cut(s) 281
Acc16I TGCGCA 1 cut(s) 2013
Acc36I ACCTGC 2 cut(s) 1422, 1917
AccB1I GGYRCC 2 cut(s) 243, 3337
AccB7I CCANNNNNTGG 4 cut(s) 311, 504, 815, 4245
AccBSI CCGCTC 1 cut(s) 1836
AccI GTMKAC 1 cut(s) 1181
AccII CGCG 4 cut(s) 1845, 2076, 4038, 4205
AclWI GGATC 3 cut(s) 15, 28, 4424
AcoI YGGCCR 1 cut(s) 4033
AcsI RAATTY 3 cut(s) 650, 3173, 3351
AcuI CTGAAG 6 cut(s) 942, 1490, 2339, 3858, 3966, 4392
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 5 cut(s) 1581, 2218, 3422, 3725, 4106
AflIII ACRYGT 1 cut(s) 3720
AhlI ACTAGT 1 cut(s) 3317
AjnI CCWGG 6 cut(s) 304, 1630, 1977, 1985, 3324, 4303
AleI CACNNNNGTG 1 cut(s) 1431
Alw21I GWGCWC 2 cut(s) 3451, 4008
Alw26I GTCTC 9 cut(s) 564, 840, 1490, 1777, 1968, 2836, 3429, 3825, 4584
Alw44I GTGCAC 1 cut(s) 3447
AlwI GGATC 3 cut(s) 15, 28, 4424
AlwNI CAGNNNCTG 3 cut(s) 520, 773, 1577
Ama87I CYCGRG 3 cut(s) 671, 1542, 2262
AoxI GGCC 6 cut(s) 228, 1531, 2056, 3150, 3312, 4033
ApaLI GTGCAC 1 cut(s) 3447
ApoI RAATTY 3 cut(s) 650, 3173, 3351
ArsI GACNNNNNNTTYG 6 cut(s) 749, 781, 1913, 1945, 4433, 4465
AseI ATTAAT 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 1440
AspLEI GCGC 6 cut(s) 50, 246, 1847, 2014, 3540, 4040
AspS9I GGNCC 5 cut(s) 229, 2393, 2872, 3151, 4336
AsuC2I CCSGG 5 cut(s) 672, 673, 1543, 1544, 3342
AsuII TTCGAA 2 cut(s) 1919, 3383
AsuNHI GCTAGC 1 cut(s) 4513
AvaI CYCGRG 3 cut(s) 671, 1542, 2262
AvaII GGWCC 3 cut(s) 2393, 2872, 4336
AxyI CCTNAGG 2 cut(s) 144, 1867
BaeGI GKGCMC 1 cut(s) 3451
BaeI ACNNNNGTAYC 2 cut(s) 2208, 2241
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 2 cut(s) 243, 3337
BanII GRGCYC 4 cut(s) 1611, 2027, 4114, 4492
BauI CACGAG 3 cut(s) 1119, 1203, 3953
BbsI GAAGAC 5 cut(s) 192, 710, 1399, 1496, 2333
Bbv12I GWGCWC 2 cut(s) 3451, 4008
BceAI ACGGC 1 cut(s) 4000
BciT130I CCWGG 6 cut(s) 306, 1632, 1979, 1987, 3326, 4305
BciVI GTATCC 2 cut(s) 383, 3203
BclI TGATCA 2 cut(s) 1207, 2977
BcnI CCSGG 5 cut(s) 672, 673, 1543, 1544, 3342
BcoDI GTCTC 9 cut(s) 564, 840, 1490, 1777, 1968, 2836, 3429, 3825, 4584
BcuI ACTAGT 1 cut(s) 3317
BfmI CTRYAG 2 cut(s) 378, 1099
BfoI RGCGCY 3 cut(s) 51, 247, 3541
BfuAI ACCTGC 2 cut(s) 1422, 1917
BfuI GTATCC 2 cut(s) 383, 3203
BglI GCCNNNNNGGC 1 cut(s) 2000
BglII AGATCT 1 cut(s) 113
BlpI GCTNAGC 1 cut(s) 1463
Bme18I GGWCC 3 cut(s) 2393, 2872, 4336
BmeT110I CYCGRG 3 cut(s) 671, 1542, 2262
BmgT120I GGNCC 5 cut(s) 229, 2393, 2872, 3151, 4336
BmiI GGNNCC 6 cut(s) 22, 166, 245, 1370, 2394, 3339
BmrI ACTGGG 2 cut(s) 471, 1089
BmtI GCTAGC 1 cut(s) 4517
BmuI ACTGGG 2 cut(s) 471, 1089
BpiI GAAGAC 5 cut(s) 192, 710, 1399, 1496, 2333
BplI GAGNNNNNCTC 2 cut(s) 3995, 4027
BpmI CTGGAG 1 cut(s) 1179
Bpu10I CCTNAGC 2 cut(s) 3309, 3598
Bpu1102I GCTNAGC 1 cut(s) 1463
Bpu14I TTCGAA 2 cut(s) 1919, 3383
BpuEI CTTGAG 3 cut(s) 1024, 1329, 1436
BpuMI CCSGG 5 cut(s) 672, 673, 1543, 1544, 3342
BsaAI YACGTR 2 cut(s) 1429, 1938
BsaBI GATNNNNATC 1 cut(s) 4656
BsaHI GRCGYC 1 cut(s) 244
BsaI GGTCTC 2 cut(s) 564, 1968
BsaJI CCNNGG 9 cut(s) 503, 670, 671, 1542, 1543, 1631, 1817, 3341, 4030
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bse21I CCTNAGG 2 cut(s) 144, 1867
Bse3DI GCAATG 2 cut(s) 550, 1393
Bse8I GATNNNNATC 1 cut(s) 4656
BseBI CCWGG 6 cut(s) 306, 1632, 1979, 1987, 3326, 4305
BseDI CCNNGG 9 cut(s) 503, 670, 671, 1542, 1543, 1631, 1817, 3341, 4030
BseJI GATNNNNATC 1 cut(s) 4656
BseMI GCAATG 2 cut(s) 550, 1393
BseRI GAGGAG 3 cut(s) 1503, 2486, 3004
BseSI GKGCMC 1 cut(s) 3451
BseYI CCCAGC 2 cut(s) 2018, 2497
BsgI GTGCAG 2 cut(s) 783, 3459
Bsh1236I CGCG 4 cut(s) 1845, 2076, 4038, 4205
BshFI GGCC 6 cut(s) 230, 1533, 2058, 3152, 3314, 4035
BshNI GGYRCC 2 cut(s) 243, 3337
BsiHKAI GWGCWC 2 cut(s) 3451, 4008
BsiHKCI CYCGRG 3 cut(s) 671, 1542, 2262
BsiSI CCGG 5 cut(s) 672, 1543, 1559, 2065, 3341
BslFI GGGAC 2 cut(s) 4154, 4421
BsmAI GTCTC 9 cut(s) 564, 840, 1490, 1777, 1968, 2836, 3429, 3825, 4584
BsmBI CGTCTC 3 cut(s) 840, 1490, 3825
BsmFI GGGAC 2 cut(s) 4154, 4421
BsmI GAATGC 7 cut(s) 906, 1231, 1880, 1996, 3199, 3805, 4234
BsnI GGCC 6 cut(s) 230, 1533, 2058, 3152, 3314, 4035
Bso31I GGTCTC 2 cut(s) 564, 1968
BsoBI CYCGRG 3 cut(s) 671, 1542, 2262
Bsp119I TTCGAA 2 cut(s) 1919, 3383
Bsp1286I GDGCHC 6 cut(s) 1611, 2027, 3451, 4008, 4114, 4492
Bsp1407I TGTACA 3 cut(s) 1579, 3420, 4104
Bsp1720I GCTNAGC 1 cut(s) 1463
Bsp19I CCATGG 1 cut(s) 1817
Bsp68I TCGCGA 1 cut(s) 2076
BspANI GGCC 6 cut(s) 230, 1533, 2058, 3152, 3314, 4035
BspFNI CGCG 4 cut(s) 1845, 2076, 4038, 4205
BspHI TCATGA 5 cut(s) 550, 591, 2344, 3262, 3864
BspLI GGNNCC 6 cut(s) 22, 166, 245, 1370, 2394, 3339
BspMI ACCTGC 2 cut(s) 1422, 1917
BspOI GCTAGC 1 cut(s) 4517
BspPI GGATC 3 cut(s) 15, 28, 4424
BspQI GCTCTTC 2 cut(s) 1084, 1446
BspT104I TTCGAA 2 cut(s) 1919, 3383
BspT107I GGYRCC 2 cut(s) 243, 3337
BspTNI GGTCTC 2 cut(s) 564, 1968
BsrBI CCGCTC 1 cut(s) 1836
BsrDI GCAATG 2 cut(s) 550, 1393
BsrGI TGTACA 3 cut(s) 1579, 3420, 4104
BssECI CCNNGG 9 cut(s) 503, 670, 671, 1542, 1543, 1631, 1817, 3341, 4030
BssNAI GTATAC 1 cut(s) 1182
BssNI GRCGYC 1 cut(s) 244
BssSI CACGAG 3 cut(s) 1119, 1203, 3953
BssT1I CCWWGG 3 cut(s) 503, 1817, 4030
Bst1107I GTATAC 1 cut(s) 1182
Bst2BI CACGAG 3 cut(s) 1119, 1203, 3953
Bst2UI CCWGG 6 cut(s) 306, 1632, 1979, 1987, 3326, 4305
Bst4CI ACNGT 8 cut(s) 353, 366, 666, 1103, 3074, 3230, 3304, 3457
Bst6I CTCTTC 9 cut(s) 1084, 1446, 1596, 1760, 1771, 1807, 2242, 3024, 4467
BstACI GRCGYC 1 cut(s) 244
BstAPI GCANNNNNTGC 4 cut(s) 155, 1315, 2009, 3305
BstAUI TGTACA 3 cut(s) 1579, 3420, 4104
BstBAI YACGTR 2 cut(s) 1429, 1938
BstBI TTCGAA 2 cut(s) 1919, 3383
BstDSI CCRYGG 1 cut(s) 1817
BstEII GGTNACC 1 cut(s) 1981
BstFNI CGCG 4 cut(s) 1845, 2076, 4038, 4205
BstH2I RGCGCY 3 cut(s) 51, 247, 3541
BstHHI GCGC 6 cut(s) 50, 246, 1847, 2014, 3540, 4040
BstMAI GTCTC 9 cut(s) 564, 840, 1490, 1777, 1968, 2836, 3429, 3825, 4584
BstNI CCWGG 6 cut(s) 306, 1632, 1979, 1987, 3326, 4305
BstNSI RCATGY 4 cut(s) 1318, 2812, 3662, 3724
BstPI GGTNACC 1 cut(s) 1981
BstSFI CTRYAG 2 cut(s) 378, 1099
BstSLI GKGCMC 1 cut(s) 3451
BstUI CGCG 4 cut(s) 1845, 2076, 4038, 4205
BstV2I GAAGAC 5 cut(s) 192, 710, 1399, 1496, 2333
BstX2I RGATCY 2 cut(s) 20, 113
BstXI CCANNNNNNTGG 2 cut(s) 866, 3811
BstYI RGATCY 2 cut(s) 20, 113
BstZ17I GTATAC 1 cut(s) 1182
Bsu36I CCTNAGG 2 cut(s) 144, 1867
BsuI GTATCC 2 cut(s) 383, 3203
BsuRI GGCC 6 cut(s) 230, 1533, 2058, 3152, 3314, 4035
BtgI CCRYGG 1 cut(s) 1817
BtsI GCAGTG 2 cut(s) 391, 1479
BtsIMutI CAGTG 7 cut(s) 358, 391, 718, 1108, 1479, 2413, 4250
BtuMI TCGCGA 1 cut(s) 2076
BveI ACCTGC 2 cut(s) 1422, 1917
CaiI CAGNNNCTG 3 cut(s) 520, 773, 1577
CciI TCATGA 5 cut(s) 550, 591, 2344, 3262, 3864
CfoI GCGC 6 cut(s) 50, 246, 1847, 2014, 3540, 4040
Cfr13I GGNCC 5 cut(s) 229, 2393, 2872, 3151, 4336
Cfr9I CCCGGG 2 cut(s) 671, 1542
CseI GACGC 1 cut(s) 3842
CsiI ACCWGGT 3 cut(s) 304, 1977, 4303
Csp6I GTAC 5 cut(s) 1580, 2217, 3421, 3724, 4105
CspCI CAANNNNNGTGG 2 cut(s) 221, 256
CviQI GTAC 5 cut(s) 1580, 2217, 3421, 3724, 4105
DinI GGCGCC 1 cut(s) 245
DraI TTTAAA 1 cut(s) 75
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 4033
Eam1104I CTCTTC 9 cut(s) 1084, 1446, 1596, 1760, 1771, 1807, 2242, 3024, 4467
EarI CTCTTC 9 cut(s) 1084, 1446, 1596, 1760, 1771, 1807, 2242, 3024, 4467
EciI GGCGGA 1 cut(s) 1828
Eco130I CCWWGG 3 cut(s) 503, 1817, 4030
Eco24I GRGCYC 4 cut(s) 1611, 2027, 4114, 4492
Eco31I GGTCTC 2 cut(s) 564, 1968
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 3 cut(s) 2393, 2872, 4336
Eco57I CTGAAG 6 cut(s) 942, 1490, 2339, 3858, 3966, 4392
Eco81I CCTNAGG 2 cut(s) 144, 1867
Eco88I CYCGRG 3 cut(s) 671, 1542, 2262
Eco91I GGTNACC 1 cut(s) 1981
EcoO109I RGGNCCY 2 cut(s) 2393, 3151
EcoO65I GGTNACC 1 cut(s) 1981
EcoRII CCWGG 6 cut(s) 304, 1630, 1977, 1985, 3324, 4303
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 3 cut(s) 503, 1817, 4030
EcoT22I ATGCAT 3 cut(s) 489, 1231, 1320
EcoT38I GRGCYC 4 cut(s) 1611, 2027, 4114, 4492
EgeI GGCGCC 1 cut(s) 245
EheI GGCGCC 1 cut(s) 245
ErhI CCWWGG 3 cut(s) 503, 1817, 4030
Esp3I CGTCTC 3 cut(s) 840, 1490, 3825
FalI AAGNNNNNCTT 6 cut(s) 1033, 1065, 1242, 1274, 2037, 2069
FaqI GGGAC 2 cut(s) 4154, 4421
FauI CCCGC 3 cut(s) 322, 1185, 1850
FauNDI CATATG 4 cut(s) 489, 1320, 2326, 2485
FbaI TGATCA 2 cut(s) 1207, 2977
FblI GTMKAC 1 cut(s) 1181
FriOI GRGCYC 4 cut(s) 1611, 2027, 4114, 4492
FspI TGCGCA 1 cut(s) 2013
GlaI GCGC 6 cut(s) 49, 245, 1846, 2013, 3539, 4039
GsaI CCCAGC 2 cut(s) 2022, 2501
GsuI CTGGAG 1 cut(s) 1179
HaeII RGCGCY 3 cut(s) 51, 247, 3541
HaeIII GGCC 6 cut(s) 230, 1533, 2058, 3152, 3314, 4035
HapII CCGG 5 cut(s) 672, 1543, 1559, 2065, 3341
HgaI GACGC 1 cut(s) 3842
HhaI GCGC 6 cut(s) 50, 246, 1847, 2014, 3540, 4040
Hin1I GRCGYC 1 cut(s) 244
Hin6I GCGC 6 cut(s) 48, 244, 1845, 2012, 3538, 4038
HinP1I GCGC 6 cut(s) 48, 244, 1845, 2012, 3538, 4038
HincII GTYRAC 4 cut(s) 495, 1863, 3400, 3718
HindII GTYRAC 4 cut(s) 495, 1863, 3400, 3718
HindIII AAGCTT 2 cut(s) 2542, 4089
HpaI GTTAAC 2 cut(s) 1863, 3400
HpaII CCGG 5 cut(s) 672, 1543, 1559, 2065, 3341
HpyCH4III ACNGT 8 cut(s) 353, 366, 666, 1103, 3074, 3230, 3304, 3457
HpyCH4IV ACGT 2 cut(s) 1428, 1937
HpySE526I ACGT 2 cut(s) 1428, 1937
Hsp92I GRCGYC 1 cut(s) 244
HspAI GCGC 6 cut(s) 48, 244, 1845, 2012, 3538, 4038
KasI GGCGCC 1 cut(s) 243
Ksp22I TGATCA 2 cut(s) 1207, 2977
KspAI GTTAAC 2 cut(s) 1863, 3400
LguI GCTCTTC 2 cut(s) 1084, 1446
LmnI GCTCC 9 cut(s) 258, 775, 1144, 1490, 1841, 3017, 3783, 4683, 4694
MabI ACCWGGT 3 cut(s) 304, 1977, 4303
MaeII ACGT 2 cut(s) 1428, 1937
MaeIII GTNAC 8 cut(s) 300, 1586, 1981, 2171, 2680, 3230, 3737, 3895
MbiI CCGCTC 1 cut(s) 1836
MfeI CAATTG 1 cut(s) 2988
MflI RGATCY 2 cut(s) 20, 113
MhlI GDGCHC 6 cut(s) 1611, 2027, 3451, 4008, 4114, 4492
Mly113I GGCGCC 1 cut(s) 244
MlyI GAGTC 8 cut(s) 206, 308, 973, 1111, 2087, 3870, 4170, 4190
Mph1103I ATGCAT 3 cut(s) 489, 1231, 1320
MroXI GAANNNNTTC 1 cut(s) 1440
MspA1I CMGCKG 2 cut(s) 962, 1306
MspI CCGG 5 cut(s) 672, 1543, 1559, 2065, 3341
MunI CAATTG 1 cut(s) 2988
Mva1269I GAATGC 7 cut(s) 906, 1231, 1880, 1996, 3199, 3805, 4234
MvaI CCWGG 6 cut(s) 306, 1632, 1979, 1987, 3326, 4305
MvnI CGCG 4 cut(s) 1845, 2076, 4038, 4205
NarI GGCGCC 1 cut(s) 244
NciI CCSGG 5 cut(s) 672, 673, 1543, 1544, 3342
NcoI CCATGG 1 cut(s) 1817
NdeI CATATG 4 cut(s) 489, 1320, 2326, 2485
NheI GCTAGC 1 cut(s) 4513
NlaIV GGNNCC 6 cut(s) 22, 166, 245, 1370, 2394, 3339
NmuCI GTSAC 4 cut(s) 300, 1981, 2680, 3230
NruI TCGCGA 1 cut(s) 2076
NsbI TGCGCA 1 cut(s) 2013
NsiI ATGCAT 3 cut(s) 489, 1231, 1320
NspI RCATGY 4 cut(s) 1318, 2812, 3662, 3724
NspV TTCGAA 2 cut(s) 1919, 3383
OliI CACNNNNGTG 1 cut(s) 1431
PagI TCATGA 5 cut(s) 550, 591, 2344, 3262, 3864
PciI ACATGT 1 cut(s) 3720
PciSI GCTCTTC 2 cut(s) 1084, 1446
PcsI WCGNNNNNNNCGW 1 cut(s) 4666
PctI GAATGC 7 cut(s) 906, 1231, 1880, 1996, 3199, 3805, 4234
PdmI GAANNNNTTC 1 cut(s) 1440
PfeI GAWTC 8 cut(s) 517, 588, 751, 986, 1349, 2743, 2930, 3259
PflFI GACNNNGTC 3 cut(s) 763, 1979, 2593
PflMI CCANNNNNTGG 4 cut(s) 311, 504, 815, 4245
PleI GAGTC 8 cut(s) 205, 307, 973, 1111, 2086, 3869, 4169, 4190
PluTI GGCGCC 1 cut(s) 247
PpsI GAGTC 8 cut(s) 205, 307, 973, 1111, 2086, 3869, 4169, 4190
Ppu21I YACGTR 2 cut(s) 1429, 1938
PpuMI RGGWCCY 1 cut(s) 2393
PscI ACATGT 1 cut(s) 3720
PshBI ATTAAT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 2510
Psp5II RGGWCCY 1 cut(s) 2393
Psp6I CCWGG 6 cut(s) 304, 1630, 1977, 1985, 3324, 4303
PspEI GGTNACC 1 cut(s) 1981
PspFI CCCAGC 2 cut(s) 2018, 2497
PspGI CCWGG 6 cut(s) 304, 1630, 1977, 1985, 3324, 4303
PspN4I GGNNCC 6 cut(s) 22, 166, 245, 1370, 2394, 3339
PspPI GGNCC 5 cut(s) 229, 2393, 2872, 3151, 4336
PspPPI RGGWCCY 1 cut(s) 2393
PstNI CAGNNNCTG 3 cut(s) 520, 773, 1577
PsuI RGATCY 2 cut(s) 20, 113
PsyI GACNNNGTC 3 cut(s) 763, 1979, 2593
PvuII CAGCTG 2 cut(s) 962, 1306
RruI TCGCGA 1 cut(s) 2076
RsaI GTAC 5 cut(s) 1581, 2218, 3422, 3725, 4106
RsaNI GTAC 5 cut(s) 1580, 2217, 3421, 3724, 4105
SapI GCTCTTC 2 cut(s) 1084, 1446
Sau96I GGNCC 5 cut(s) 229, 2393, 2872, 3151, 4336
SchI GAGTC 8 cut(s) 206, 308, 973, 1111, 2087, 3870, 4170, 4190
SduI GDGCHC 6 cut(s) 1611, 2027, 3451, 4008, 4114, 4492
SexAI ACCWGGT 3 cut(s) 304, 1977, 4303
SfcI CTRYAG 2 cut(s) 378, 1099
SfoI GGCGCC 1 cut(s) 245
SfuI TTCGAA 2 cut(s) 1919, 3383
SinI GGWCC 3 cut(s) 2393, 2872, 4336
SmaI CCCGGG 2 cut(s) 673, 1544
SmlI CTYRAG 3 cut(s) 1039, 1344, 1451
SmoI CTYRAG 3 cut(s) 1039, 1344, 1451
SpeI ACTAGT 1 cut(s) 3317
SspDI GGCGCC 1 cut(s) 243
SspI AATATT 1 cut(s) 3683
StyI CCWWGG 3 cut(s) 503, 1817, 4030
TaaI ACNGT 8 cut(s) 353, 366, 666, 1103, 3074, 3230, 3304, 3457
TaiI ACGT 2 cut(s) 1431, 1940
TaqII GACCGA 1 cut(s) 1986
TatI WGTACW 3 cut(s) 1579, 3420, 4104
TauI GCSGC 4 cut(s) 1031, 2071, 3620, 4038
TfiI GAWTC 8 cut(s) 517, 588, 751, 986, 1349, 2743, 2930, 3259
TscAI CASTG 7 cut(s) 358, 391, 718, 1108, 1486, 2413, 4250
TseFI GTSAC 4 cut(s) 300, 1981, 2680, 3230
Tsp45I GTSAC 4 cut(s) 300, 1981, 2680, 3230
TspGWI ACGGA 4 cut(s) 921, 1237, 2104, 4429
TspMI CCCGGG 2 cut(s) 671, 1542
TspRI CASTG 7 cut(s) 358, 391, 718, 1108, 1486, 2413, 4250
Tth111I GACNNNGTC 3 cut(s) 763, 1979, 2593
Van91I CCANNNNNTGG 4 cut(s) 311, 504, 815, 4245
VneI GTGCAC 1 cut(s) 3447
VpaK11BI GGWCC 3 cut(s) 2393, 2872, 4336
VspI ATTAAT 1 cut(s) 408
XapI RAATTY 3 cut(s) 650, 3173, 3351
XbaI TCTAGA 4 cut(s) 578, 935, 3122, 3412
XceI RCATGY 4 cut(s) 1318, 2812, 3662, 3724
XcmI CCANNNNNNNNNTGG 2 cut(s) 3322, 4311
XmaI CCCGGG 2 cut(s) 671, 1542
XmiI GTMKAC 1 cut(s) 1181
XmnI GAANNNNTTC 1 cut(s) 1440
Zsp2I ATGCAT 3 cut(s) 489, 1231, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.